MD10G1138000.v1.1

At1g04910-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0010779
Physical Location & Seq
Reverse (-)
22252865 .. 22260794
7930 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1138000.v1.1.491

Sequence Viewer

Length: 1851 bp
ATGAAGAAGAAGAAGAGAGACGCAATCAACGGCCACAGCTGCATCAACATCACCGCCACCGCCCTCGGCGGCGTCGTTTTCAAAACCTCTGTTCTCCGGCGACACTTCCGCCGTATCCGCCACCACGTGTTCCCGCTAATCTCCGCCGTTTCCGGTTGCCTGCTCCTCTTCCTCGCCGCCTTCTCTGTCCTCGCTCCGTCGCCGGTTCTGCATCCTCATGATCACCTCTCGCTCTTGCGGCACCTCACCTCGAATGACAATGGCGACGTTGAGGTCTGGCCGAATTCGGATGAGACAACGAAGTTTCGCGTGACGGAGAGTGGGGGAAGTTCAGGTCGAGATTTATGGAGTACAAGGGAATCGGAGCTTTTCTATAGCTGCAGCAATGCCGCCGATAACTTTGCAAGTAAACCGTCTGCTGATGCGAAAACTCATTCGAATCGCTACTTGTTGATTGCTACTAGTGGAGGATTGAACCAACAAAGAACAGGGATAACAGATGCTGTCGTTGCAGCTTATATCTTGAACGCTACTCTTGTTGTTCCCAAGCTGGACCAGAAATCGTATTGGAAGGATTCCAGCAACTTCGATGAAATCTTTGATGTAGACTGGTTTATATCATCTCTCTCGAAGGATGTTAAAATCATTAAAGAGCTTCCAACAAAGGGAGGGAAACCAATGAGTGTGCATACCACGCGAGTCCCAAGGAAGTGTAATGAAAAATGTTACCAGAACCGCTTGGTGCCCATTCTTAATAAAAAGCATGCTGTTCAGCTCACAAAATTTGATTACAGACTTTCAAATAGGCTGGATTCAGATTTACAAAAGCTGAGATGTAGAGCCAATTATCAAGCCTTGAAATTTACCGACACGATTAGTGAAATGGGTAAAAAGTTGGTTGAGAGAATGAGAATGAAAAGCAAGCATTTCATTGCCCTGCATCTGAGGTTTGAGCCTGATATGCTGGCGTTTTCTGGATGCGATTATGGTGGGGGAGAAAAGGAAAGGAAAGAACTTGGCAAACTTCGGAAAAGGTGGAAAACTTTACATGCAAGCAACCCTGACAAGGTACGAAGACATGGCAGATGCCCGCTAACTCCAGAGGAAGTTGGTCTCATGCTTAGAGCGCTGGGTTTCGGAAGTGATATTCACTTGTATGTGGCATCCGGTGAAGTATATGGAGGTGAAGAGACAATGGCACCACTAAAAGCACTTTTCCCAAACTTCCACACAAAAGAGACTTTATTAAGTAAGAAGGAGTTGGCACCATTTTCATCCTTCTCTTCTCGAATGGCTGCACTGGACTTCGTTGTTTGCGATGAAAGTGATGTTTTCATCACCAACAACAATGGCAATATGGCTAAAATGTTAGCTGGTCGAAGGAGATACTTTGGTCACAAACCAACAATCCGTCCAAATGCTAAGAAATTGTCTCCGTTGTTCATAAATCGTAATAACATGACATGGGAAGAATTTGCATCGAGGCTTCGAACAAGTCAAATTGGATTCATGGGAGAGCCAAATGAGATAAAGCTCGGCAGGGGTGAGTTCCACGAAAACCCAGCAGCTTGCATTTGTAGCGCATCTAACACAAAGGCTAAGGAAGTTCCAATTCCTCAAAATGAAAGTCCCGATATCCTTAAAATAGATAAGGAGGATTATACGAACAAGGACAGTGGTTATGTGACAGATGAGCAAGTAACTGAGGACGAGCAAGATTGGTCTGAGGCGGATTATACTGAAATTGCAAATCGGTCTCTAGGGAAAGTGCTGCCTAGCGTAAGAGTTTCAGATCCAAGTCTTCTACTTAAGTCCGACCAACCAGAACTCGACGAATTCTTTTCAGACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
O-FucT

Protein Analysis

617

Amino Acids

69.46

Weight (kDa)

9.04

Isoelectric Point (pI)

40.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
O-FucT PF10250 149 - 463 1.4e-103 GDP-fucose protein O-fucosyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 272
AccB1I GGYRCC 4 cut(s) 240, 742, 1198, 1264
AccI GTMKAC 1 cut(s) 606
AccII CGCG 2 cut(s) 309, 697
AclWI GGATC 1 cut(s) 1787
AcoI YGGCCR 2 cut(s) 31, 278
AcsI RAATTY 5 cut(s) 283, 782, 860, 1472, 1835
AcvI CACGTG 1 cut(s) 127
AcyI GRCGYC 1 cut(s) 72
AdeI CACNNNGTG 1 cut(s) 127
AfaI GTAC 2 cut(s) 352, 1071
AfeI AGCGCT 1 cut(s) 1128
AfiI CCNNNNNNNGG 2 cut(s) 665, 1066
AflII CTTAAG 1 cut(s) 1808
AflIII ACRYGT 1 cut(s) 126
AgsI TTSAA 5 cut(s) 82, 475, 526, 801, 859
AhlI ACTAGT 1 cut(s) 461
Alw26I GTCTC 7 cut(s) 12, 287, 1118, 1184, 1232, 1437, 1761
AlwI GGATC 1 cut(s) 1787
AlwNI CAGNNNCTG 1 cut(s) 503
Aor51HI AGCGCT 1 cut(s) 1128
AoxI GGCC 2 cut(s) 31, 278
ApeKI GCWGC 7 cut(s) 39, 378, 381, 512, 1295, 1565, 1771
ApoI RAATTY 5 cut(s) 283, 782, 860, 1472, 1835
ArsI GACNNNNNNTTYG 2 cut(s) 1612, 1644
AspLEI GCGC 2 cut(s) 1129, 1583
AspS9I GGNCC 1 cut(s) 553
AsuHPI GGTGA 7 cut(s) 43, 215, 238, 1181, 1196, 1330, 1556
AsuII TTCGAA 2 cut(s) 437, 1489
AvaII GGWCC 1 cut(s) 553
BaeGI GKGCMC 1 cut(s) 747
BaeI ACNNNNGTAYC 2 cut(s) 1378, 1411
BanI GGYRCC 4 cut(s) 240, 742, 1198, 1264
BbrPI CACGTG 1 cut(s) 127
BbsI GAAGAC 2 cut(s) 1081, 1793
BbvI GCAGC 7 cut(s) 26, 365, 393, 524, 1282, 1577, 1758
BceAI ACGGC 3 cut(s) 46, 96, 131
BcgI CGANNNNNNTGC 2 cut(s) 383, 417
BciVI GTATCC 1 cut(s) 125
BclI TGATCA 1 cut(s) 220
BcoDI GTCTC 7 cut(s) 12, 287, 1118, 1184, 1232, 1437, 1761
BcuI ACTAGT 1 cut(s) 461
BfaI CTAG 3 cut(s) 462, 1760, 1776
BfmI CTRYAG 2 cut(s) 373, 379
BfoI RGCGCY 1 cut(s) 1130
BfrI CTTAAG 1 cut(s) 1808
BfuI GTATCC 1 cut(s) 125
Bme18I GGWCC 1 cut(s) 553
BmgT120I GGNCC 1 cut(s) 553
BmiI GGNNCC 4 cut(s) 242, 744, 1200, 1266
BpiI GAAGAC 2 cut(s) 1081, 1793
BpmI CTGGAG 1 cut(s) 1083
Bpu10I CCTNAGC 1 cut(s) 1599
Bpu14I TTCGAA 2 cut(s) 437, 1489
BsaAI YACGTR 1 cut(s) 127
BsaHI GRCGYC 1 cut(s) 72
BsaI GGTCTC 2 cut(s) 1118, 1761
BsaJI CCNNGG 2 cut(s) 64, 704
BsaWI WCCGGW 2 cut(s) 152, 1166
Bsc4I CCNNNNNNNGG 2 cut(s) 665, 1066
Bse118I RCCGGY 1 cut(s) 202
Bse1I ACTGG 2 cut(s) 614, 1305
Bse3DI GCAATG 2 cut(s) 391, 930
BseDI CCNNGG 2 cut(s) 64, 704
BseGI GGATG 6 cut(s) 211, 295, 640, 983, 1163, 1274
BseLI CCNNNNNNNGG 2 cut(s) 665, 1066
BseMI GCAATG 2 cut(s) 391, 930
BseMII CTCAG 4 cut(s) 821, 935, 1695, 1716
BseNI ACTGG 2 cut(s) 614, 1305
BseRI GAGGAG 1 cut(s) 155
BseSI GKGCMC 1 cut(s) 747
BseXI GCAGC 7 cut(s) 26, 365, 393, 524, 1282, 1577, 1758
BseYI CCCAGC 2 cut(s) 1129, 1561
BsgI GTGCAG 1 cut(s) 1281
Bsh1236I CGCG 2 cut(s) 309, 697
BshFI GGCC 2 cut(s) 33, 280
BshNI GGYRCC 4 cut(s) 240, 742, 1198, 1264
BsiSI CCGG 4 cut(s) 97, 153, 203, 1167
BslFI GGGAC 2 cut(s) 686, 1614
BslI CCNNNNNNNGG 2 cut(s) 665, 1066
BsmAI GTCTC 7 cut(s) 12, 287, 1118, 1184, 1232, 1437, 1761
BsmBI CGTCTC 1 cut(s) 12
BsmFI GGGAC 2 cut(s) 686, 1614
BsnI GGCC 2 cut(s) 33, 280
Bso31I GGTCTC 2 cut(s) 1118, 1761
Bsp119I TTCGAA 2 cut(s) 437, 1489
Bsp1286I GDGCHC 1 cut(s) 747
Bsp143I GATC 2 cut(s) 220, 1792
BspANI GGCC 2 cut(s) 33, 280
BspCNI CTCAG 4 cut(s) 822, 936, 1696, 1717
BspFNI CGCG 2 cut(s) 309, 697
BspHI TCATGA 1 cut(s) 217
BspLI GGNNCC 4 cut(s) 242, 744, 1200, 1266
BspMAI CTGCAG 1 cut(s) 383
BspPI GGATC 1 cut(s) 1787
BspT104I TTCGAA 2 cut(s) 437, 1489
BspT107I GGYRCC 4 cut(s) 240, 742, 1198, 1264
BspTI CTTAAG 1 cut(s) 1808
BspTNI GGTCTC 2 cut(s) 1118, 1761
BsrDI GCAATG 2 cut(s) 391, 930
BsrFI RCCGGY 1 cut(s) 202
BsrI ACTGG 2 cut(s) 614, 1305
BssAI RCCGGY 1 cut(s) 202
BssECI CCNNGG 2 cut(s) 64, 704
BssMI GATC 2 cut(s) 220, 1792
BssNI GRCGYC 1 cut(s) 72
BssT1I CCWWGG 1 cut(s) 704
Bst4CI ACNGT 2 cut(s) 414, 1676
Bst6I CTCTTC 4 cut(s) 8, 173, 1182, 1288
BstACI GRCGYC 1 cut(s) 72
BstAFI CTTAAG 1 cut(s) 1808
BstBAI YACGTR 1 cut(s) 127
BstBI TTCGAA 2 cut(s) 437, 1489
BstC8I GCNNGC 7 cut(s) 161, 765, 923, 966, 1054, 1091, 1570
BstDEI CTNAG 7 cut(s) 830, 944, 1121, 1422, 1599, 1704, 1725
BstF5I GGATG 6 cut(s) 211, 295, 640, 983, 1163, 1274
BstFNI CGCG 2 cut(s) 309, 697
BstH2I RGCGCY 1 cut(s) 1130
BstHHI GCGC 2 cut(s) 1129, 1583
BstKTI GATC 2 cut(s) 223, 1795
BstMAI GTCTC 7 cut(s) 12, 287, 1118, 1184, 1232, 1437, 1761
BstMBI GATC 2 cut(s) 220, 1792
BstMWI GCNNNNNNNGC 9 cut(s) 39, 117, 208, 238, 509, 694, 961, 1126, 1578
BstNSI RCATGY 2 cut(s) 767, 1052
BstSFI CTRYAG 2 cut(s) 373, 379
BstSLI GKGCMC 1 cut(s) 747
BstUI CGCG 2 cut(s) 309, 697
BstV1I GCAGC 7 cut(s) 26, 365, 393, 524, 1282, 1577, 1758
BstV2I GAAGAC 2 cut(s) 1081, 1793
BstX2I RGATCY 1 cut(s) 1792
BstYI RGATCY 1 cut(s) 1792
BsuI GTATCC 1 cut(s) 125
BsuRI GGCC 2 cut(s) 33, 280
BtgZI GCGATG 1 cut(s) 1332
BtsCI GGATG 6 cut(s) 211, 295, 640, 983, 1163, 1274
BtsIMutI CAGTG 2 cut(s) 1298, 1681
Cac8I GCNNGC 7 cut(s) 161, 765, 923, 966, 1054, 1091, 1570
CaiI CAGNNNCTG 1 cut(s) 503
CciI TCATGA 1 cut(s) 217
CfoI GCGC 2 cut(s) 1129, 1583
Cfr10I RCCGGY 1 cut(s) 202
Cfr13I GGNCC 1 cut(s) 553
CseI GACGC 2 cut(s) 29, 61
Csp6I GTAC 2 cut(s) 351, 1070
CspCI CAANNNNNGTGG 2 cut(s) 1657, 1692
CviAII CATG 8 cut(s) 218, 764, 1049, 1079, 1117, 1459, 1464, 1510
CviQI GTAC 2 cut(s) 351, 1070
DdeI CTNAG 7 cut(s) 830, 944, 1121, 1422, 1599, 1704, 1725
DpnI GATC 2 cut(s) 222, 1794
DpnII GATC 2 cut(s) 220, 1792
DraIII CACNNNGTG 1 cut(s) 127
DrdI GACNNNNNNGTC 1 cut(s) 272
DseDI GACNNNNNNGTC 1 cut(s) 272
EaeI YGGCCR 2 cut(s) 31, 278
Eam1104I CTCTTC 4 cut(s) 8, 173, 1182, 1288
EarI CTCTTC 4 cut(s) 8, 173, 1182, 1288
EciI GGCGGA 4 cut(s) 98, 107, 133, 1745
Eco130I CCWWGG 1 cut(s) 704
Eco31I GGTCTC 2 cut(s) 1118, 1761
Eco32I GATATC 1 cut(s) 1636
Eco47I GGWCC 1 cut(s) 553
Eco47III AGCGCT 1 cut(s) 1128
Eco72I CACGTG 1 cut(s) 127
EcoRI GAATTC 2 cut(s) 283, 1835
EcoRV GATATC 1 cut(s) 1636
EcoT14I CCWWGG 1 cut(s) 704
ErhI CCWWGG 1 cut(s) 704
Esp3I CGTCTC 1 cut(s) 12
FaeI CATG 8 cut(s) 221, 767, 1052, 1082, 1120, 1462, 1467, 1513
FaqI GGGAC 2 cut(s) 686, 1614
FatI CATG 8 cut(s) 217, 763, 1048, 1078, 1116, 1458, 1463, 1509
FauI CCCGC 2 cut(s) 141, 1098
FbaI TGATCA 1 cut(s) 220
FblI GTMKAC 1 cut(s) 606
FokI GGATG 6 cut(s) 198, 302, 647, 990, 1150, 1261
FspBI CTAG 3 cut(s) 462, 1760, 1776
GlaI GCGC 2 cut(s) 1128, 1582
GsaI CCCAGC 2 cut(s) 1133, 1565
GsuI CTGGAG 1 cut(s) 1083
HaeII RGCGCY 1 cut(s) 1130
HaeIII GGCC 2 cut(s) 33, 280
HapII CCGG 4 cut(s) 97, 153, 203, 1167
HgaI GACGC 2 cut(s) 29, 61
HhaI GCGC 2 cut(s) 1129, 1583
Hin1I GRCGYC 1 cut(s) 72
Hin1II CATG 8 cut(s) 221, 767, 1052, 1082, 1120, 1462, 1467, 1513
Hin6I GCGC 2 cut(s) 1127, 1581
HinP1I GCGC 2 cut(s) 1127, 1581
HinfI GANTC 6 cut(s) 359, 439, 575, 699, 812, 1506
HpaII CCGG 4 cut(s) 97, 153, 203, 1167
HphI GGTGA 7 cut(s) 43, 215, 238, 1181, 1196, 1330, 1556
Hpy166II GTNNAC 2 cut(s) 410, 607
Hpy188III TCNNGA 8 cut(s) 218, 338, 523, 628, 975, 1100, 1287, 1631
Hpy8I GTNNAC 2 cut(s) 410, 607
Hpy99I CGWCG 4 cut(s) 77, 202, 269, 1835
HpyAV CCTTC 6 cut(s) 190, 565, 625, 1249, 1288, 1374
HpyCH4III ACNGT 2 cut(s) 414, 1676
HpyCH4IV ACGT 2 cut(s) 126, 267
HpyF10VI GCNNNNNNNGC 9 cut(s) 39, 117, 208, 238, 509, 694, 961, 1126, 1578
HpyF3I CTNAG 7 cut(s) 830, 944, 1121, 1422, 1599, 1704, 1725
HpySE526I ACGT 2 cut(s) 126, 267
Hsp92I GRCGYC 1 cut(s) 72
Hsp92II CATG 8 cut(s) 221, 767, 1052, 1082, 1120, 1462, 1467, 1513
HspAI GCGC 2 cut(s) 1127, 1581
Ksp22I TGATCA 1 cut(s) 220
Kzo9I GATC 2 cut(s) 220, 1792
LmnI GCTCC 3 cut(s) 168, 199, 364
Lsp1109I GCAGC 7 cut(s) 26, 365, 393, 524, 1282, 1577, 1758
MaeI CTAG 3 cut(s) 462, 1760, 1776
MaeII ACGT 2 cut(s) 126, 267
MaeIII GTNAC 5 cut(s) 310, 725, 1394, 1684, 1699
MalI GATC 2 cut(s) 222, 1794
MboI GATC 2 cut(s) 220, 1792
MflI RGATCY 1 cut(s) 1792
MhlI GDGCHC 1 cut(s) 747
MlyI GAGTC 1 cut(s) 708
MmeI TCCRAC 2 cut(s) 683, 1839
MseI TTAA 6 cut(s) 639, 648, 753, 1247, 1641, 1809
MslI CAYNNNNRTG 2 cut(s) 216, 1155
MspA1I CMGCKG 1 cut(s) 39
MspCI CTTAAG 1 cut(s) 1808
MspI CCGG 4 cut(s) 97, 153, 203, 1167
MvnI CGCG 2 cut(s) 309, 697
MwoI GCNNNNNNNGC 9 cut(s) 39, 117, 208, 238, 509, 694, 961, 1126, 1578
NdeII GATC 2 cut(s) 220, 1792
NlaIII CATG 8 cut(s) 221, 767, 1052, 1082, 1120, 1462, 1467, 1513
NlaIV GGNNCC 4 cut(s) 242, 744, 1200, 1266
NmeAIII GCCGAG 2 cut(s) 45, 1515
NmuCI GTSAC 3 cut(s) 310, 1394, 1684
NspI RCATGY 2 cut(s) 767, 1052
NspV TTCGAA 2 cut(s) 437, 1489
PaeI GCATGC 1 cut(s) 767
PagI TCATGA 1 cut(s) 217
PcsI WCGNNNNNNNCGW 2 cut(s) 72, 1314
PfeI GAWTC 5 cut(s) 359, 439, 575, 812, 1506
PleI GAGTC 1 cut(s) 707
PmaCI CACGTG 1 cut(s) 127
PmlI CACGTG 1 cut(s) 127
PpsI GAGTC 1 cut(s) 707
Ppu21I YACGTR 1 cut(s) 127
PspCI CACGTG 1 cut(s) 127
PspFI CCCAGC 2 cut(s) 1129, 1561
PspN4I GGNNCC 4 cut(s) 242, 744, 1200, 1266
PspPI GGNCC 1 cut(s) 553
PstI CTGCAG 1 cut(s) 383
PstNI CAGNNNCTG 1 cut(s) 503
PsuI RGATCY 1 cut(s) 1792
PvuII CAGCTG 1 cut(s) 39
RsaI GTAC 2 cut(s) 352, 1071
RsaNI GTAC 2 cut(s) 351, 1070
RseI CAYNNNNRTG 2 cut(s) 216, 1155
SaqAI TTAA 6 cut(s) 639, 648, 753, 1247, 1641, 1809
Sau3AI GATC 2 cut(s) 220, 1792
Sau96I GGNCC 1 cut(s) 553
SchI GAGTC 1 cut(s) 708
SduI GDGCHC 1 cut(s) 747
SfcI CTRYAG 2 cut(s) 373, 379
SfuI TTCGAA 2 cut(s) 437, 1489
SinI GGWCC 1 cut(s) 553
SmiMI CAYNNNNRTG 2 cut(s) 216, 1155
SmlI CTYRAG 1 cut(s) 1808
SmoI CTYRAG 1 cut(s) 1808
SpeI ACTAGT 1 cut(s) 461
SphI GCATGC 1 cut(s) 767
SspMI CTAG 3 cut(s) 462, 1760, 1776
StyI CCWWGG 1 cut(s) 704
TaaI ACNGT 2 cut(s) 414, 1676
TaiI ACGT 2 cut(s) 129, 270
TaqII GACCGA 1 cut(s) 1743
TatI WGTACW 1 cut(s) 350
TauI GCSGC 4 cut(s) 72, 179, 241, 392
TfiI GAWTC 5 cut(s) 359, 439, 575, 812, 1506
Tru1I TTAA 6 cut(s) 639, 648, 753, 1247, 1641, 1809
Tru9I TTAA 6 cut(s) 639, 648, 753, 1247, 1641, 1809
TscAI CASTG 2 cut(s) 1305, 1681
TseFI GTSAC 3 cut(s) 310, 1394, 1684
TseI GCWGC 7 cut(s) 39, 378, 381, 512, 1295, 1565, 1771
Tsp45I GTSAC 3 cut(s) 310, 1394, 1684
TspGWI ACGGA 4 cut(s) 186, 329, 1400, 1425
TspRI CASTG 2 cut(s) 1305, 1681
Vha464I CTTAAG 1 cut(s) 1808
VpaK11BI GGWCC 1 cut(s) 553
XapI RAATTY 5 cut(s) 283, 782, 860, 1472, 1835
XceI RCATGY 2 cut(s) 767, 1052
XmiI GTMKAC 1 cut(s) 606
XspI CTAG 3 cut(s) 462, 1760, 1776
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.