MD10G1136700.v1.1

At1g04910-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0010779
Physical Location & Seq
Reverse (-)
22131628 .. 22132461
834 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1136700.v1.1.491

Sequence Viewer

Length: 735 bp
ATGCTTAGAGCGTTGGGTTTCGGAAGTGATATTCACTTGTATGTGGCATCCGGTGAAGTATATGGAGGTGAAGAGACATTGGCACCACTAAAAGCACTTTTCCCAAACTTCCACACAAAAGAGACTTTATTAAGTAAGAAGGAGTTGGCACCATTTTCATCCTTCTCCTCTCGAATGGCTGCATTGGACTTCACTGTTTGCGATGAAAGTGATGTTTTCATCACCAACAACAATGGCAATATGGCTAAAATGTTAGCTGGTCGAAGGAGATACTTTGGTCACAAATCAACAATCCGTCCAAATGCTAAGAAACTGTCTCCGTTGTTCATAAATCGTAATAACATGACATGGGAAGAATTTGCATCGAGGCTTCGAACAAGTCAAATTGGATTCATGGGAGAGCCAAATGAGATAAAGCCCGGCAGGGGTGAGTTCCACGAAAACCCAGCAGCCTGCATTTGTAGCGCATCTAACACAAAGGCTAAGGAAGTTCCAATTCCTCAAAATGAAAGTCCTGATATCCTTAAAATAGACAAGGACGATTATACGAACAAGGACAGTGGTTATGCGACAGATGAGCAAGTAACTGAGGATGAGCAAGATTGTTCTGAGGCGGATTATGCTGAAATTACAAATAGGTCTCTAGGGAAAGTGCTGCCTAGTGTAAGAGTTTCAGATCAAAGTCTTCTACTTAAGTCAGACCAACCAGAACTCGAAGAATTCTTTTCAGACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
O-FucT

Protein Analysis

245

Amino Acids

27.25

Weight (kDa)

5.02

Isoelectric Point (pI)

48.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
O-FucT PF10250 1 - 91 2.1e-25 GDP-fucose protein O-fucosyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 82, 148
AciI CCGC 1 cut(s) 614
AcsI RAATTY 2 cut(s) 356, 719
AfiI CCNNNNNNNGG 1 cut(s) 425
AflII CTTAAG 1 cut(s) 692
AluBI AGCT 1 cut(s) 257
AluI AGCT 1 cut(s) 257
Alw26I GTCTC 4 cut(s) 68, 116, 321, 645
ApeKI GCWGC 3 cut(s) 179, 449, 655
ApoI RAATTY 2 cut(s) 356, 719
ArsI GACNNNNNNTTYG 2 cut(s) 496, 528
AspLEI GCGC 1 cut(s) 467
AsuC2I CCSGG 1 cut(s) 420
AsuHPI GGTGA 4 cut(s) 65, 80, 214, 440
AsuII TTCGAA 1 cut(s) 373
BaeI ACNNNNGTAYC 2 cut(s) 262, 295
BanI GGYRCC 2 cut(s) 82, 148
BbsI GAAGAC 1 cut(s) 677
BbvI GCAGC 3 cut(s) 166, 461, 642
BcnI CCSGG 1 cut(s) 420
BcoDI GTCTC 4 cut(s) 68, 116, 321, 645
BfaI CTAG 2 cut(s) 644, 660
BfrI CTTAAG 1 cut(s) 692
BisI GCNGC 3 cut(s) 180, 450, 656
BlsI GCNGC 3 cut(s) 181, 451, 657
Bme1390I CCNGG 1 cut(s) 420
BmiI GGNNCC 2 cut(s) 84, 150
BmrFI CCNGG 1 cut(s) 420
BmsI GCATC 3 cut(s) 56, 371, 476
BpiI GAAGAC 1 cut(s) 677
Bpu10I CCTNAGC 1 cut(s) 483
Bpu14I TTCGAA 1 cut(s) 373
BpuMI CCSGG 1 cut(s) 420
BsaI GGTCTC 1 cut(s) 645
BsaWI WCCGGW 1 cut(s) 50
Bsc4I CCNNNNNNNGG 1 cut(s) 425
BseGI GGATG 3 cut(s) 47, 158, 598
BseLI CCNNNNNNNGG 1 cut(s) 425
BseMII CTCAG 2 cut(s) 579, 600
BseRI GAGGAG 1 cut(s) 157
BseXI GCAGC 3 cut(s) 166, 461, 642
BseYI CCCAGC 1 cut(s) 445
BshNI GGYRCC 2 cut(s) 82, 148
BsiSI CCGG 2 cut(s) 51, 420
BslI CCNNNNNNNGG 1 cut(s) 425
BsmAI GTCTC 4 cut(s) 68, 116, 321, 645
Bso31I GGTCTC 1 cut(s) 645
Bsp119I TTCGAA 1 cut(s) 373
Bsp143I GATC 1 cut(s) 676
BspACI CCGC 1 cut(s) 614
BspCNI CTCAG 2 cut(s) 580, 601
BspLI GGNNCC 2 cut(s) 84, 150
BspT104I TTCGAA 1 cut(s) 373
BspT107I GGYRCC 2 cut(s) 82, 148
BspTI CTTAAG 1 cut(s) 692
BspTNI GGTCTC 1 cut(s) 645
BssMI GATC 1 cut(s) 676
Bst4CI ACNGT 3 cut(s) 196, 315, 560
Bst6I CTCTTC 1 cut(s) 66
BstAFI CTTAAG 1 cut(s) 692
BstBI TTCGAA 1 cut(s) 373
BstC8I GCNNGC 1 cut(s) 454
BstDEI CTNAG 5 cut(s) 5, 306, 483, 588, 609
BstF5I GGATG 3 cut(s) 47, 158, 598
BstHHI GCGC 1 cut(s) 467
BstKTI GATC 1 cut(s) 679
BstMAI GTCTC 4 cut(s) 68, 116, 321, 645
BstMBI GATC 1 cut(s) 676
BstMWI GCNNNNNNNGC 2 cut(s) 462, 620
BstSCI CCNGG 1 cut(s) 418
BstV1I GCAGC 3 cut(s) 166, 461, 642
BstV2I GAAGAC 1 cut(s) 677
BtgZI GCGATG 1 cut(s) 216
BtsCI GGATG 3 cut(s) 47, 158, 598
BtsIMutI CAGTG 2 cut(s) 192, 565
Cac8I GCNNGC 1 cut(s) 454
CfoI GCGC 1 cut(s) 467
CspCI CAANNNNNGTGG 2 cut(s) 541, 576
CviAII CATG 3 cut(s) 343, 348, 394
CviJI RGCY 8 cut(s) 179, 245, 257, 370, 403, 418, 452, 482
CviKI_1 RGCY 8 cut(s) 179, 245, 257, 370, 403, 418, 452, 482
DdeI CTNAG 5 cut(s) 5, 306, 483, 588, 609
DpnI GATC 1 cut(s) 678
DpnII GATC 1 cut(s) 676
Eam1104I CTCTTC 1 cut(s) 66
EarI CTCTTC 1 cut(s) 66
EciI GGCGGA 1 cut(s) 629
Eco31I GGTCTC 1 cut(s) 645
Eco32I GATATC 1 cut(s) 520
EcoRI GAATTC 1 cut(s) 719
EcoRV GATATC 1 cut(s) 520
FaeI CATG 3 cut(s) 346, 351, 397
FatI CATG 3 cut(s) 342, 347, 393
Fnu4HI GCNGC 3 cut(s) 180, 450, 656
FokI GGATG 3 cut(s) 34, 145, 605
Fsp4HI GCNGC 3 cut(s) 180, 450, 656
FspBI CTAG 2 cut(s) 644, 660
GlaI GCGC 1 cut(s) 466
GluI GCNGC 3 cut(s) 180, 450, 656
GsaI CCCAGC 1 cut(s) 449
HapII CCGG 2 cut(s) 51, 420
HhaI GCGC 1 cut(s) 467
Hin1II CATG 3 cut(s) 346, 351, 397
Hin6I GCGC 1 cut(s) 465
HinP1I GCGC 1 cut(s) 465
HinfI GANTC 1 cut(s) 390
HpaII CCGG 2 cut(s) 51, 420
HphI GGTGA 4 cut(s) 65, 80, 214, 440
Hpy188I TCNGA 5 cut(s) 23, 610, 676, 700, 730
Hpy188III TCNNGA 2 cut(s) 171, 515
HpyAV CCTTC 3 cut(s) 133, 172, 258
HpyCH4III ACNGT 3 cut(s) 196, 315, 560
HpyCH4V TGCA 3 cut(s) 182, 362, 456
HpyF10VI GCNNNNNNNGC 2 cut(s) 462, 620
HpyF3I CTNAG 5 cut(s) 5, 306, 483, 588, 609
Hsp92II CATG 3 cut(s) 346, 351, 397
HspAI GCGC 1 cut(s) 465
Kzo9I GATC 1 cut(s) 676
LpnPI CCDG 8 cut(s) 64, 243, 409, 433, 459, 466, 528, 720
Lsp1109I GCAGC 3 cut(s) 166, 461, 642
LweI GCATC 3 cut(s) 56, 371, 476
MaeI CTAG 2 cut(s) 644, 660
MaeIII GTNAC 2 cut(s) 278, 583
MalI GATC 1 cut(s) 678
MboI GATC 1 cut(s) 676
MboII GAAGA 4 cut(s) 83, 365, 677, 728
MluCI AATT 5 cut(s) 356, 384, 495, 627, 719
MnlI CCTC 6 cut(s) 59, 178, 360, 510, 583, 604
MseI TTAA 3 cut(s) 131, 525, 693
MslI CAYNNNNRTG 1 cut(s) 39
MspCI CTTAAG 1 cut(s) 692
MspI CCGG 2 cut(s) 51, 420
MspR9I CCNGG 1 cut(s) 420
MwoI GCNNNNNNNGC 2 cut(s) 462, 620
NciI CCSGG 1 cut(s) 420
NdeII GATC 1 cut(s) 676
NlaIII CATG 3 cut(s) 346, 351, 397
NlaIV GGNNCC 2 cut(s) 84, 150
NmuCI GTSAC 1 cut(s) 278
NspV TTCGAA 1 cut(s) 373
PfeI GAWTC 1 cut(s) 390
PkrI GCNGC 3 cut(s) 181, 451, 657
PspFI CCCAGC 1 cut(s) 445
PspN4I GGNNCC 2 cut(s) 84, 150
RseI CAYNNNNRTG 1 cut(s) 39
SaqAI TTAA 3 cut(s) 131, 525, 693
SatI GCNGC 3 cut(s) 180, 450, 656
Sau3AI GATC 1 cut(s) 676
ScrFI CCNGG 1 cut(s) 420
SetI ASST 3 cut(s) 70, 259, 641
SfaNI GCATC 3 cut(s) 56, 371, 476
SfuI TTCGAA 1 cut(s) 373
SmiMI CAYNNNNRTG 1 cut(s) 39
SmlI CTYRAG 1 cut(s) 692
SmoI CTYRAG 1 cut(s) 692
Sse9I AATT 5 cut(s) 356, 384, 495, 627, 719
SsiI CCGC 1 cut(s) 614
SspMI CTAG 2 cut(s) 644, 660
StyD4I CCNGG 1 cut(s) 418
TaaI ACNGT 3 cut(s) 196, 315, 560
TaqI TCGA 5 cut(s) 172, 262, 365, 373, 714
TasI AATT 5 cut(s) 356, 384, 495, 627, 719
TfiI GAWTC 1 cut(s) 390
Tru1I TTAA 3 cut(s) 131, 525, 693
Tru9I TTAA 3 cut(s) 131, 525, 693
TscAI CASTG 2 cut(s) 199, 565
TseFI GTSAC 1 cut(s) 278
TseI GCWGC 3 cut(s) 179, 449, 655
Tsp45I GTSAC 1 cut(s) 278
TspDTI ATGAA 6 cut(s) 147, 208, 219, 316, 382, 522
TspGWI ACGGA 2 cut(s) 284, 309
TspRI CASTG 2 cut(s) 199, 565
Vha464I CTTAAG 1 cut(s) 692
XapI RAATTY 2 cut(s) 356, 719
XspI CTAG 2 cut(s) 644, 660
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.