MD05G1134800.v1.1

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0014273
Physical Location & Seq
Reverse (-)
26317056 .. 26318337
1282 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1134800.v1.1.491

Sequence Viewer

Length: 933 bp
ATGATTGGTGGCTTTGAGTGTTGTGAGGAAGCTCGAATTGGGATGCAGATGTTCCGAAAGATGGTATCTGAGCTTGGCATTGGACCGGATGGTGTTACTACGGTCAGCGTTCTTAAAGCATGCACTAATTTGAAGGGCTTGATCATGGGAATGGCGGTCCACGGCGATGCAGATTCTGCACTAATAGTTTTCGAGGCAATGCTGCGAAGGAATAAGTCAGTGCACTGCATAACAATCAGATCAGGAGACGAATCAAACGAATTGCTCCTAAATTCTCTCATGGATGCTTATGCAAAGTGCAACGATGTTGAACTTGCATGGAAACTTTTTAGAGGGAAGAAGATAAGGGATGTGGTTTCATGGAGCAATATGATTGGAGGTTTTGCCAGTTCTTCAGTTGAATTGAAGAGGTCGAATTGGGCTCATGGAATTGCTATTAGAAGAGGTTTGGCAGCACAAGTAGCCATCAGAACTGCAATTGTTGACATGTACTCGAAATGTGGGGCAATAGGAGAATCAAGAAAGGCATTTGGACAAATTTGTGAAGAAAAAAAAAACATTGTGTCATTGAGTGCCATGATAGCTGGGTATGGAATGAATGGTCTTGGCCATGAAGCGCTATCTTTGCTTGCAGAAATGAAGCTGTATGATTTGAAACCAAATGCAGTGACCATTCTTTCTCTGCTATCTGCATGTAGCCATGGAGGTTTAATCGAAGAGGACCTTTCCTTGTTCGCTTCAATGGTTCAAAATCACGAAGTTGAACGAAGATTGAAACATTACACTTGTGTAGTTGACATGTTGAGTCGAGCAGGAAAGCTTGTAACAGCAATGGAATTTATAAGAAAAGTCCCGGAAAGTTTGATGGCCTCCGGAACAAGTAATGCTTGGGGTGCATTATTGAGTGCTTGCATGTGGAATTTTTCTGGTTGA

Protein Analysis

311

Amino Acids

33.7

Weight (kDa)

8.29

Isoelectric Point (pI)

35.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 185 - 233 1.8e-08 PPR repeat family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014273)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G17210
fragaria_vesca FvH4_2g09980 FvH4_2g09980
malus_domestica MD05G1134800.v1.1 MD10G1139900.v1.1
prunus_persica Prupe.8G178700_v2.0.a1
pyrus_communis pycom10g11880
rosa_chinensis RchiOBHm_Chr6g0266861
rosa_laevigata RLG00000014068
rosa_multiflora Rmu_sc0013959.1_g000001
rosa_roxburghii Rroxscaffold_7G00201400
rosa_rugosa Rorug06G0027700
rosa_samantha Rh6AG148700 Rh6BG149800 Rh6CG144700 Rh6DG133900
rosa_wichuraiana Rw6G012870

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 842
AccIII TCCGGA 1 cut(s) 872
AciI CCGC 1 cut(s) 155
AcoI YGGCCR 1 cut(s) 607
AcsI RAATTY 4 cut(s) 271, 537, 836, 919
AcuI CTGAAG 1 cut(s) 378
AfaI GTAC 1 cut(s) 491
AfeI AGCGCT 1 cut(s) 618
AfiI CCNNNNNNNGG 1 cut(s) 61
AflIII ACRYGT 2 cut(s) 486, 798
AgsI TTSAA 9 cut(s) 133, 311, 401, 406, 655, 741, 749, 764, 775
AjuI GAANNNNNNNTTGG 2 cut(s) 21, 53
AluBI AGCT 5 cut(s) 32, 73, 584, 643, 820
AluI AGCT 5 cut(s) 32, 73, 584, 643, 820
Alw21I GWGCWC 1 cut(s) 225
Alw26I GTCTC 1 cut(s) 240
Alw44I GTGCAC 1 cut(s) 221
AlwNI CAGNNNCTG 1 cut(s) 176
Aor13HI TCCGGA 1 cut(s) 872
Aor51HI AGCGCT 1 cut(s) 618
AoxI GGCC 2 cut(s) 607, 867
ApaLI GTGCAC 1 cut(s) 221
ApeKI GCWGC 2 cut(s) 202, 452
ApoI RAATTY 4 cut(s) 271, 537, 836, 919
AspLEI GCGC 1 cut(s) 619
AspS9I GGNCC 3 cut(s) 83, 157, 721
AsuC2I CCSGG 1 cut(s) 854
AvaII GGWCC 3 cut(s) 83, 157, 721
BaeGI GKGCMC 1 cut(s) 225
BalI TGGCCA 1 cut(s) 609
BanII GRGCYC 1 cut(s) 424
Bbv12I GWGCWC 1 cut(s) 225
BbvI GCAGC 2 cut(s) 189, 464
BccI CCATC 4 cut(s) 55, 83, 473, 859
BceAI ACGGC 1 cut(s) 178
BclI TGATCA 1 cut(s) 141
BcnI CCSGG 1 cut(s) 854
BcoDI GTCTC 1 cut(s) 240
BfoI RGCGCY 1 cut(s) 620
BisI GCNGC 2 cut(s) 203, 453
BlsI GCNGC 2 cut(s) 204, 454
Bme1390I CCNGG 1 cut(s) 854
Bme18I GGWCC 3 cut(s) 83, 157, 721
BmgT120I GGNCC 3 cut(s) 83, 157, 721
BmrFI CCNGG 1 cut(s) 854
BmsI GCATC 3 cut(s) 33, 157, 274
BpuMI CCSGG 1 cut(s) 854
BsaJI CCNNGG 2 cut(s) 160, 700
BsaWI WCCGGW 2 cut(s) 85, 872
Bsc4I CCNNNNNNNGG 1 cut(s) 61
Bse1I ACTGG 1 cut(s) 387
Bse3DI GCAATG 2 cut(s) 204, 837
BseAI TCCGGA 1 cut(s) 872
BseDI CCNNGG 2 cut(s) 160, 700
BseGI GGATG 4 cut(s) 48, 94, 289, 355
BseLI CCNNNNNNNGG 1 cut(s) 61
BseMI GCAATG 2 cut(s) 204, 837
BseMII CTCAG 1 cut(s) 60
BseNI ACTGG 1 cut(s) 387
BseSI GKGCMC 1 cut(s) 225
BseXI GCAGC 2 cut(s) 189, 464
BseYI CCCAGC 1 cut(s) 584
BsgI GTGCAG 1 cut(s) 162
BshFI GGCC 2 cut(s) 609, 869
BsiHKAI GWGCWC 1 cut(s) 225
BsiSI CCGG 3 cut(s) 86, 854, 873
BslFI GGGAC 1 cut(s) 836
BslI CCNNNNNNNGG 1 cut(s) 61
BsmAI GTCTC 1 cut(s) 240
BsmBI CGTCTC 1 cut(s) 240
BsmFI GGGAC 1 cut(s) 836
BsnI GGCC 2 cut(s) 609, 869
Bsp1286I GDGCHC 2 cut(s) 225, 424
Bsp13I TCCGGA 1 cut(s) 872
Bsp143I GATC 2 cut(s) 141, 239
Bsp19I CCATGG 1 cut(s) 700
BspACI CCGC 1 cut(s) 155
BspANI GGCC 2 cut(s) 609, 869
BspCNI CTCAG 1 cut(s) 61
BspEI TCCGGA 1 cut(s) 872
BsrDI GCAATG 2 cut(s) 204, 837
BsrI ACTGG 1 cut(s) 387
BssECI CCNNGG 2 cut(s) 160, 700
BssMI GATC 2 cut(s) 141, 239
BssT1I CCWWGG 1 cut(s) 700
Bst4CI ACNGT 1 cut(s) 103
Bst6I CTCTTC 3 cut(s) 401, 436, 711
BstAPI GCANNNNNTGC 1 cut(s) 176
BstC8I GCNNGC 3 cut(s) 121, 630, 910
BstDEI CTNAG 1 cut(s) 69
BstDSI CCRYGG 2 cut(s) 160, 700
BstF5I GGATG 4 cut(s) 48, 94, 289, 355
BstH2I RGCGCY 1 cut(s) 620
BstHHI GCGC 1 cut(s) 619
BstKTI GATC 2 cut(s) 144, 242
BstMAI GTCTC 1 cut(s) 240
BstMBI GATC 2 cut(s) 141, 239
BstMWI GCNNNNNNNGC 5 cut(s) 176, 461, 581, 625, 893
BstNSI RCATGY 5 cut(s) 123, 490, 696, 802, 916
BstSCI CCNGG 1 cut(s) 852
BstSLI GKGCMC 1 cut(s) 225
BstV1I GCAGC 2 cut(s) 189, 464
BsuRI GGCC 2 cut(s) 609, 869
BtgI CCRYGG 2 cut(s) 160, 700
BtgZI GCGATG 1 cut(s) 180
BtsCI GGATG 4 cut(s) 48, 94, 289, 355
BtsI GCAGTG 2 cut(s) 223, 672
BtsIMutI CAGTG 3 cut(s) 223, 225, 672
Cac8I GCNNGC 3 cut(s) 121, 630, 910
CaiI CAGNNNCTG 1 cut(s) 176
CfoI GCGC 1 cut(s) 619
Cfr13I GGNCC 3 cut(s) 83, 157, 721
Csp6I GTAC 1 cut(s) 490
CviQI GTAC 1 cut(s) 490
DdeI CTNAG 1 cut(s) 69
DpnI GATC 2 cut(s) 143, 241
DpnII GATC 2 cut(s) 141, 239
EaeI YGGCCR 1 cut(s) 607
Eam1104I CTCTTC 3 cut(s) 401, 436, 711
EarI CTCTTC 3 cut(s) 401, 436, 711
Eco130I CCWWGG 1 cut(s) 700
Eco24I GRGCYC 1 cut(s) 424
Eco47I GGWCC 3 cut(s) 83, 157, 721
Eco47III AGCGCT 1 cut(s) 618
Eco57I CTGAAG 1 cut(s) 378
EcoO109I RGGNCCY 1 cut(s) 721
EcoT14I CCWWGG 1 cut(s) 700
EcoT38I GRGCYC 1 cut(s) 424
ErhI CCWWGG 1 cut(s) 700
Esp3I CGTCTC 1 cut(s) 240
FalI AAGNNNNNCTT 4 cut(s) 708, 740, 871, 903
FaqI GGGAC 1 cut(s) 836
FbaI TGATCA 1 cut(s) 141
Fnu4HI GCNGC 2 cut(s) 203, 453
FokI GGATG 4 cut(s) 55, 101, 296, 362
FriOI GRGCYC 1 cut(s) 424
Fsp4HI GCNGC 2 cut(s) 203, 453
GlaI GCGC 1 cut(s) 618
GluI GCNGC 2 cut(s) 203, 453
GsaI CCCAGC 1 cut(s) 588
HaeII RGCGCY 1 cut(s) 620
HaeIII GGCC 2 cut(s) 609, 869
HapII CCGG 3 cut(s) 86, 854, 873
HhaI GCGC 1 cut(s) 619
Hin6I GCGC 1 cut(s) 617
HinP1I GCGC 1 cut(s) 617
HincII GTYRAC 2 cut(s) 484, 796
HindII GTYRAC 2 cut(s) 484, 796
HindIII AAGCTT 1 cut(s) 818
HinfI GANTC 4 cut(s) 173, 251, 515, 805
HpaII CCGG 3 cut(s) 86, 854, 873
Hpy166II GTNNAC 4 cut(s) 160, 223, 484, 796
Hpy188I TCNGA 4 cut(s) 56, 70, 239, 470
Hpy188III TCNNGA 4 cut(s) 243, 519, 755, 873
Hpy8I GTNNAC 4 cut(s) 160, 223, 484, 796
HpyAV CCTTC 2 cut(s) 127, 201
HpyCH4III ACNGT 1 cut(s) 103
HpyF10VI GCNNNNNNNGC 5 cut(s) 176, 461, 581, 625, 893
HpyF3I CTNAG 1 cut(s) 69
HspAI GCGC 1 cut(s) 617
Kpn2I TCCGGA 1 cut(s) 872
Ksp22I TGATCA 1 cut(s) 141
Kzo9I GATC 2 cut(s) 141, 239
LmnI GCTCC 2 cut(s) 270, 363
LpnPI CCDG 8 cut(s) 99, 228, 400, 570, 798, 867, 886, 912
Lsp1109I GCAGC 2 cut(s) 189, 464
LweI GCATC 3 cut(s) 33, 157, 274
MaeIII GTNAC 3 cut(s) 94, 667, 823
MalI GATC 2 cut(s) 143, 241
MboI GATC 2 cut(s) 141, 239
MboII GAAGA 8 cut(s) 349, 352, 384, 418, 453, 557, 728, 780
MfeI CAATTG 1 cut(s) 477
MhlI GDGCHC 2 cut(s) 225, 424
MlsI TGGCCA 1 cut(s) 609
MluNI TGGCCA 1 cut(s) 609
MlyI GAGTC 1 cut(s) 814
MnlI CCTC 9 cut(s) 19, 187, 326, 371, 402, 437, 698, 712, 880
Mox20I TGGCCA 1 cut(s) 609
MroI TCCGGA 1 cut(s) 872
MscI TGGCCA 1 cut(s) 609
MseI TTAA 2 cut(s) 114, 710
MslI CAYNNNNRTG 2 cut(s) 149, 165
Msp20I TGGCCA 1 cut(s) 609
MspI CCGG 3 cut(s) 86, 854, 873
MspR9I CCNGG 1 cut(s) 854
MunI CAATTG 1 cut(s) 477
MwoI GCNNNNNNNGC 5 cut(s) 176, 461, 581, 625, 893
NciI CCSGG 1 cut(s) 854
NcoI CCATGG 1 cut(s) 700
NdeII GATC 2 cut(s) 141, 239
NmuCI GTSAC 1 cut(s) 667
NspI RCATGY 5 cut(s) 123, 490, 696, 802, 916
PaeI GCATGC 1 cut(s) 123
PciI ACATGT 2 cut(s) 486, 798
PcsI WCGNNNNNNNCGW 1 cut(s) 255
PfeI GAWTC 3 cut(s) 173, 251, 515
PfoI TCCNGGA 1 cut(s) 852
PkrI GCNGC 2 cut(s) 204, 454
PleI GAGTC 1 cut(s) 813
PpsI GAGTC 1 cut(s) 813
PpuMI RGGWCCY 1 cut(s) 721
PscI ACATGT 2 cut(s) 486, 798
PsiI TTATAA 1 cut(s) 842
Psp5II RGGWCCY 1 cut(s) 721
PspFI CCCAGC 1 cut(s) 584
PspPI GGNCC 3 cut(s) 83, 157, 721
PspPPI RGGWCCY 1 cut(s) 721
PstNI CAGNNNCTG 1 cut(s) 176
RsaI GTAC 1 cut(s) 491
RsaNI GTAC 1 cut(s) 490
RseI CAYNNNNRTG 2 cut(s) 149, 165
SaqAI TTAA 2 cut(s) 114, 710
SatI GCNGC 2 cut(s) 203, 453
Sau3AI GATC 2 cut(s) 141, 239
Sau96I GGNCC 3 cut(s) 83, 157, 721
SchI GAGTC 1 cut(s) 814
ScrFI CCNGG 1 cut(s) 854
SduI GDGCHC 2 cut(s) 225, 424
SfaNI GCATC 3 cut(s) 33, 157, 274
SinI GGWCC 3 cut(s) 83, 157, 721
SmiMI CAYNNNNRTG 2 cut(s) 149, 165
SphI GCATGC 1 cut(s) 123
SsiI CCGC 1 cut(s) 155
StyD4I CCNGG 1 cut(s) 852
StyI CCWWGG 1 cut(s) 700
TaaI ACNGT 1 cut(s) 103
TaqI TCGA 6 cut(s) 34, 192, 413, 494, 714, 808
TatI WGTACW 1 cut(s) 489
TfiI GAWTC 3 cut(s) 173, 251, 515
Tru1I TTAA 2 cut(s) 114, 710
Tru9I TTAA 2 cut(s) 114, 710
TscAI CASTG 3 cut(s) 225, 230, 672
TseFI GTSAC 1 cut(s) 667
TseI GCWGC 2 cut(s) 202, 452
Tsp45I GTSAC 1 cut(s) 667
TspDTI ATGAA 4 cut(s) 348, 611, 627, 653
TspRI CASTG 3 cut(s) 225, 230, 672
VneI GTGCAC 1 cut(s) 221
VpaK11BI GGWCC 3 cut(s) 83, 157, 721
XapI RAATTY 4 cut(s) 271, 537, 836, 919
XceI RCATGY 5 cut(s) 123, 490, 696, 802, 916
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.