MD05G1143200.v1.1

Rare lipoprotein A (RlpA)-like double-psi beta-barrel

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0013383
Physical Location & Seq
Forward (+)
27412672 .. 27414950
2279 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1143200.v1.1.491

Sequence Viewer

Length: 768 bp
ATGGGAGGAGCCTTTCAACTTCAAGATGCTGCATTTCTCTTCTGGGTTTTACTGTGCGGCAGCATTGCTGCTGCTCAGAATGAAGAAGAATGGAAGACTGCCACTGCAACATATTCCAACGAAAGTGATGGGTCCATCATTACTGAAGGTGCGTGTGGTTATGGTGACCTTCACAGGATCAGCTATGGGAAGCACAGTGCTGGGGTGAGTGGCATTTTGTTCAACAAAGGGAGAACCTGTGGCGCTTGTTATGAACTGAGATGTGTTGACCACATCTTGTGGTGCCTGCAAGGGAGCCCCTCTGTTATTCTCACTGCTACAGATTTCTGCCCTCCGAATTTCGGGCTCTCAAAAGATTATGGTGGCTGGTGCAATTTCCCCAATGAACACTTTGAGATGTCTGAGGCAGCATTTGGTGAAATTGCAAAGAAAAAAGCCGATATTGTGCCAGTTCAATATAGGAGGGTGAAGTGTGAGAGAATTGGAGGGATAAGGTTCATGGTGGGTGGGAGTTCTCACTTCTACCAAGTCCTAATCACCAATGTAGGTTCAGATGGGGAGGTTCTTGCTGTGAAAGTGAAGGGTTCAAGGACCGGGTGGATACCGATGGCCAGGAACTGGGGACAGAACTGGCAGAGCAATGTCAATCTTAAAGGGCAGCCTCTTTCGTTCGAGGTAACTGTAAGTAGTGGAAGAACACTAACATCTTACAATGTTGCTCCAGCAAACTGGCAGTTTGGCCAGACATTTGAAGGGAAACAGTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005618 GO:0005623 GO:0006949 GO:0008150 GO:0009628 GO:0009653 GO:0009987 GO:0016020 GO:0030312 GO:0032502 GO:0044464 GO:0048646 GO:0048856 GO:0050896 GO:0071944 GO:0080167
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DPBB_1 Pollen_allerg_1

Protein Analysis

256

Amino Acids

27.94

Weight (kDa)

6.93

Isoelectric Point (pI)

35.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DPBB_1 PF03330 68 - 153 7.4e-18 Lytic transglycolase
Expansin_C PF01357 164 - 241 7.7e-28 Expansin C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013383)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G38210
fragaria_vesca FvH4_2g17030 FvH4_2g17030
malus_domestica MD05G1143200.v1.1 MD10G1143000.v1.1
prunus_persica Prupe.8G182000_v2.0.a1
pyrus_communis pycom05g13770 pycom10g12290
rosa_chinensis RchiOBHm_Chr6g0281211
rosa_laevigata RLG00000012985
rosa_multiflora Rmu_sc0027531.1_g000003
rosa_roxburghii Rroxscaffold_7G00187220
rosa_rugosa Rorug06G0139700
rosa_samantha Rh6AG251600 Rh6BG254900 Rh6CG254400 Rh6DG246200
rosa_wichuraiana Rw6G021850

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 282
AccB7I CCANNNNNTGG 1 cut(s) 618
AciI CCGC 1 cut(s) 57
AclWI GGATC 1 cut(s) 185
AcoI YGGCCR 2 cut(s) 609, 739
AcsI RAATTY 1 cut(s) 337
AcuI CTGAAG 1 cut(s) 165
AfiI CCNNNNNNNGG 2 cut(s) 341, 618
AgsI TTSAA 6 cut(s) 17, 23, 223, 455, 588, 752
AjnI CCWGG 1 cut(s) 611
AluBI AGCT 1 cut(s) 183
AluI AGCT 1 cut(s) 183
AlwI GGATC 1 cut(s) 185
AlwNI CAGNNNCTG 1 cut(s) 618
AoxI GGCC 2 cut(s) 609, 739
ApeKI GCWGC 6 cut(s) 29, 60, 68, 71, 407, 658
ApoI RAATTY 1 cut(s) 337
Asp700I GAANNNNTTC 1 cut(s) 761
AspLEI GCGC 1 cut(s) 245
AspS9I GGNCC 2 cut(s) 132, 591
AsuC2I CCSGG 1 cut(s) 595
AsuHPI GGTGA 5 cut(s) 176, 217, 428, 478, 529
AvaII GGWCC 2 cut(s) 132, 591
BalI TGGCCA 2 cut(s) 611, 741
BanI GGYRCC 1 cut(s) 282
BanII GRGCYC 2 cut(s) 299, 348
BbsI GAAGAC 1 cut(s) 101
BbvI GCAGC 6 cut(s) 16, 55, 58, 72, 419, 670
BccI CCATC 4 cut(s) 122, 143, 548, 601
BciT130I CCWGG 1 cut(s) 613
BciVI GTATCC 1 cut(s) 594
BcnI CCSGG 1 cut(s) 595
BfmI CTRYAG 1 cut(s) 318
BfoI RGCGCY 1 cut(s) 246
BfuI GTATCC 1 cut(s) 594
BisI GCNGC 7 cut(s) 30, 58, 61, 69, 72, 408, 659
BlsI GCNGC 7 cut(s) 31, 59, 62, 70, 73, 409, 660
Bme1390I CCNGG 2 cut(s) 595, 613
Bme18I GGWCC 2 cut(s) 132, 591
BmgT120I GGNCC 2 cut(s) 132, 591
BmiI GGNNCC 4 cut(s) 10, 133, 284, 296
BmrFI CCNGG 2 cut(s) 595, 613
BmrI ACTGGG 1 cut(s) 628
BmsI GCATC 1 cut(s) 16
BmuI ACTGGG 1 cut(s) 628
BpiI GAAGAC 1 cut(s) 101
BpmI CTGGAG 1 cut(s) 705
BpuMI CCSGG 1 cut(s) 595
BsaXI ACNNNNNCTCC 2 cut(s) 223, 253
Bsc4I CCNNNNNNNGG 2 cut(s) 341, 618
Bse1I ACTGG 4 cut(s) 449, 623, 635, 734
Bse3DI GCAATG 2 cut(s) 63, 646
BseBI CCWGG 1 cut(s) 613
BseLI CCNNNNNNNGG 2 cut(s) 341, 618
BseMI GCAATG 2 cut(s) 63, 646
BseMII CTCAG 3 cut(s) 89, 248, 393
BseNI ACTGG 4 cut(s) 449, 623, 635, 734
BseRI GAGGAG 1 cut(s) 21
BseXI GCAGC 6 cut(s) 16, 55, 58, 72, 419, 670
BseYI CCCAGC 1 cut(s) 200
BshFI GGCC 2 cut(s) 611, 741
BshNI GGYRCC 1 cut(s) 282
BsiSI CCGG 1 cut(s) 594
BslFI GGGAC 1 cut(s) 636
BslI CCNNNNNNNGG 2 cut(s) 341, 618
BsmFI GGGAC 1 cut(s) 636
BsnI GGCC 2 cut(s) 611, 741
Bsp1286I GDGCHC 2 cut(s) 299, 348
Bsp143I GATC 1 cut(s) 177
BspACI CCGC 1 cut(s) 57
BspANI GGCC 2 cut(s) 611, 741
BspCNI CTCAG 3 cut(s) 88, 249, 394
BspLI GGNNCC 4 cut(s) 10, 133, 284, 296
BspPI GGATC 1 cut(s) 185
BspT107I GGYRCC 1 cut(s) 282
BsrDI GCAATG 2 cut(s) 63, 646
BsrI ACTGG 4 cut(s) 449, 623, 635, 734
BssMI GATC 1 cut(s) 177
Bst2UI CCWGG 1 cut(s) 613
Bst4CI ACNGT 4 cut(s) 54, 197, 682, 762
Bst6I CTCTTC 1 cut(s) 44
BstC8I GCNNGC 1 cut(s) 287
BstDEI CTNAG 3 cut(s) 75, 257, 402
BstEII GGTNACC 1 cut(s) 164
BstH2I RGCGCY 1 cut(s) 246
BstHHI GCGC 1 cut(s) 245
BstKTI GATC 1 cut(s) 180
BstMBI GATC 1 cut(s) 177
BstNI CCWGG 1 cut(s) 613
BstPI GGTNACC 1 cut(s) 164
BstSCI CCNGG 2 cut(s) 593, 611
BstSFI CTRYAG 1 cut(s) 318
BstV1I GCAGC 6 cut(s) 16, 55, 58, 72, 419, 670
BstV2I GAAGAC 1 cut(s) 101
BstXI CCANNNNNNTGG 1 cut(s) 729
BsuI GTATCC 1 cut(s) 594
BsuRI GGCC 2 cut(s) 611, 741
BtsI GCAGTG 2 cut(s) 102, 312
BtsIMutI CAGTG 3 cut(s) 102, 202, 312
Cac8I GCNNGC 1 cut(s) 287
CaiI CAGNNNCTG 1 cut(s) 618
CfoI GCGC 1 cut(s) 245
Cfr13I GGNCC 2 cut(s) 132, 591
CviAII CATG 1 cut(s) 499
CviJI RGCY 9 cut(s) 11, 183, 297, 346, 366, 437, 611, 661, 741
CviKI_1 RGCY 9 cut(s) 11, 183, 297, 346, 366, 437, 611, 661, 741
DdeI CTNAG 3 cut(s) 75, 257, 402
DpnI GATC 1 cut(s) 179
DpnII GATC 1 cut(s) 177
EaeI YGGCCR 2 cut(s) 609, 739
Eam1104I CTCTTC 1 cut(s) 44
EarI CTCTTC 1 cut(s) 44
Eco24I GRGCYC 2 cut(s) 299, 348
Eco47I GGWCC 2 cut(s) 132, 591
Eco57I CTGAAG 1 cut(s) 165
Eco91I GGTNACC 1 cut(s) 164
EcoO65I GGTNACC 1 cut(s) 164
EcoRII CCWGG 1 cut(s) 611
EcoT38I GRGCYC 2 cut(s) 299, 348
FaeI CATG 1 cut(s) 502
FaiI YATR 7 cut(s) 112, 162, 186, 252, 360, 459, 500
FaqI GGGAC 1 cut(s) 636
FatI CATG 1 cut(s) 498
Fnu4HI GCNGC 7 cut(s) 30, 58, 61, 69, 72, 408, 659
FriOI GRGCYC 2 cut(s) 299, 348
Fsp4HI GCNGC 7 cut(s) 30, 58, 61, 69, 72, 408, 659
GlaI GCGC 1 cut(s) 244
GluI GCNGC 7 cut(s) 30, 58, 61, 69, 72, 408, 659
GsaI CCCAGC 1 cut(s) 204
GsuI CTGGAG 1 cut(s) 705
HaeII RGCGCY 1 cut(s) 246
HaeIII GGCC 2 cut(s) 611, 741
HapII CCGG 1 cut(s) 594
HhaI GCGC 1 cut(s) 245
Hin1II CATG 1 cut(s) 502
Hin6I GCGC 1 cut(s) 243
HinP1I GCGC 1 cut(s) 243
HincII GTYRAC 1 cut(s) 268
HindII GTYRAC 1 cut(s) 268
HpaII CCGG 1 cut(s) 594
HphI GGTGA 5 cut(s) 176, 217, 428, 478, 529
Hpy166II GTNNAC 1 cut(s) 268
Hpy188I TCNGA 5 cut(s) 78, 336, 403, 553, 767
Hpy188III TCNNGA 1 cut(s) 23
Hpy8I GTNNAC 1 cut(s) 268
HpyAV CCTTC 4 cut(s) 140, 179, 574, 746
HpyCH4III ACNGT 4 cut(s) 54, 197, 682, 762
HpyCH4V TGCA 5 cut(s) 32, 107, 289, 372, 425
HpyF3I CTNAG 3 cut(s) 75, 257, 402
Hsp92II CATG 1 cut(s) 502
HspAI GCGC 1 cut(s) 243
Kzo9I GATC 1 cut(s) 177
LmnI GCTCC 3 cut(s) 8, 294, 724
Lsp1109I GCAGC 6 cut(s) 16, 55, 58, 72, 419, 670
LweI GCATC 1 cut(s) 16
MaeIII GTNAC 2 cut(s) 164, 676
MalI GATC 1 cut(s) 179
MboI GATC 1 cut(s) 177
MboII GAAGA 5 cut(s) 31, 95, 98, 106, 705
MhlI GDGCHC 2 cut(s) 299, 348
MlsI TGGCCA 2 cut(s) 611, 741
MluCI AATT 4 cut(s) 337, 373, 420, 480
MluNI TGGCCA 2 cut(s) 611, 741
MmeI TCCRAC 1 cut(s) 141
MnlI CCTC 8 cut(s) 310, 342, 397, 456, 479, 553, 667, 672
Mox20I TGGCCA 2 cut(s) 611, 741
MroXI GAANNNNTTC 1 cut(s) 761
MscI TGGCCA 2 cut(s) 611, 741
MseI TTAA 1 cut(s) 651
Msp20I TGGCCA 2 cut(s) 611, 741
MspI CCGG 1 cut(s) 594
MspR9I CCNGG 2 cut(s) 595, 613
MvaI CCWGG 1 cut(s) 613
NciI CCSGG 1 cut(s) 595
NdeII GATC 1 cut(s) 177
NlaIII CATG 1 cut(s) 502
NlaIV GGNNCC 4 cut(s) 10, 133, 284, 296
NmuCI GTSAC 1 cut(s) 164
PdmI GAANNNNTTC 1 cut(s) 761
PflMI CCANNNNNTGG 1 cut(s) 618
PkrI GCNGC 7 cut(s) 31, 59, 62, 70, 73, 409, 660
Psp6I CCWGG 1 cut(s) 611
PspEI GGTNACC 1 cut(s) 164
PspFI CCCAGC 1 cut(s) 200
PspGI CCWGG 1 cut(s) 611
PspN4I GGNNCC 4 cut(s) 10, 133, 284, 296
PspPI GGNCC 2 cut(s) 132, 591
PstNI CAGNNNCTG 1 cut(s) 618
SaqAI TTAA 1 cut(s) 651
SatI GCNGC 7 cut(s) 30, 58, 61, 69, 72, 408, 659
Sau3AI GATC 1 cut(s) 177
Sau96I GGNCC 2 cut(s) 132, 591
ScrFI CCNGG 2 cut(s) 595, 613
SduI GDGCHC 2 cut(s) 299, 348
SetI ASST 8 cut(s) 151, 171, 185, 239, 497, 550, 564, 678
SfaNI GCATC 1 cut(s) 16
SfcI CTRYAG 1 cut(s) 318
SinI GGWCC 2 cut(s) 132, 591
Sse9I AATT 4 cut(s) 337, 373, 420, 480
SsiI CCGC 1 cut(s) 57
StyD4I CCNGG 2 cut(s) 593, 611
TaaI ACNGT 4 cut(s) 54, 197, 682, 762
TaqI TCGA 1 cut(s) 672
TasI AATT 4 cut(s) 337, 373, 420, 480
TauI GCSGC 1 cut(s) 60
Tru1I TTAA 1 cut(s) 651
Tru9I TTAA 1 cut(s) 651
TscAI CASTG 3 cut(s) 109, 202, 319
TseFI GTSAC 1 cut(s) 164
TseI GCWGC 6 cut(s) 29, 60, 68, 71, 407, 658
Tsp45I GTSAC 1 cut(s) 164
TspDTI ATGAA 4 cut(s) 96, 267, 399, 487
TspRI CASTG 3 cut(s) 109, 202, 319
Van91I CCANNNNNTGG 1 cut(s) 618
VpaK11BI GGWCC 2 cut(s) 132, 591
XapI RAATTY 1 cut(s) 337
XmnI GAANNNNTTC 1 cut(s) 761
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.