Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr05

Orthogroup ID

OG0010957

Physical Location & Seq

Genomic Coordinates Forward (+)

29266159 .. 29269225

Gene Structure 3067 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD05G1163500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1875 bp

ATGGAATCCAAACAAACAGCGGCAGCAGCTAGTACCACCCGATTGAGCCCCTTCAAGTACTCGAAACGGGTGCTCTTGAAAACCATCTTGGAGAGAAGGGATGGTGGTTTGGAGTTAATTGGTGAGAGGGTTGTGATTGGAGGGTGGGTGAAGTCCTCCAAGGAGGTCAGGAAAGAGCCTGTGCCACGTATAGATGGTGATCATGTGCCGGGTGAACCCGAACCAAGGTCAGACGTCAGCTGCGTCGAAATTCTTCAGTCTCGGATACCTTTTTTACGAACAATTGTCAAGGTTTTGGGAGGAGGAAGTGGTGGCAATTATCCTCTTAAGGACAAGTTGGAGGCGGCGCTTCATAAGCCTCCGCCACCTTCCACTGTCTTCTTGAAAGTTGGGGATGGTTCTTGCGTTGCAAGCCTTCAGGTTGTCGTTGAATCCTCCCTGGCTCCCCCAGGCCAGCTCTTGCATACTGGAACTTGCGTCATAGTGGAAGGCTTGCTGCAGAAACCTTCGGTACTACAGGGCAAACATGTTATCGAGCTGAAAGTGGATCATGTCCATCACATCGGAATAGTAGAATATAGCACGTATCCATTATCCAAAAAACGTGTGCCTCTAGATAAACTAAGGGAATTTTGCCACATTAGGCAGCGGACAACTACGGTGCAATCTGTTATGCGAATCCGCAATGCCTTGGAGTTTGCCGCTCACACATTTTGTCAAAACAATGGTTTTCTTTCGGTGCAAGTACCCATTATAACAACTACAGACGGCAAAGGATCCAGCGAAAAGTTCCATGTCACTAGCCTTTTCACCGAAGCAGGTGAAAAGGAGGAGCCTAAGAAAGCTGTTGCTGAAATTGATGGTGTTAGTCTAGATGTTATCAAGGCTGCAGTAAAAGAGAAAAGTAACTTACTAGAGGAACTCAAGAGGACAGACAGCAATAAGGAAGCAGTAGCTGCTGCAATGCAGGACTTAAACAAAACAAATGAGTTAGCATCACAACTGGAAGCAAGAGAGAAATCAAAGCCGAAAACTTCTCAGAAGGTTGACAATGTTAAATCTTCTGAGGGTTACTTCTCTAGCCCAACTTATCTAACCGCTTCTGGCCGGATGCATCTGGAGAGCTACGCTTGTGCCCTTGGAAATGTGTACTCATTTGGGCCAAGATTTCGAGCAGAGAAAGGAGAGTCCCCAAAACATGCTGCAGAAATGTGGATGCTTGAAGTTGAATTGGCCTTTTCAGAATTAGAGGAAGCAATCAATTGTGCTGATGACTTTTTCAAGTTCCTCTGCAAATGGGTAGTGGAAAATTGTTCCGAAGATATGAAATTTGTTTCTCAACGAATCGACAAAACCAGAATTGATCGCCTTCAGTCGATCATTTCAAGTTCATTTGAAAGAGTTTCCTACAGTGAAGCATTAGATGCATTAAAGAAGGTTACAGACAAGAAAATTGAGACAAAAATTGAGTGGGGTGCTTCACTAACTGAAGCAGATCTAAGTTATTTGGCTGATGAGATATACAAGAAGCCTGTAATTATATACAATTATCCAAAAGAAACCAAGCCATTTTATGTCCGGTTGAACGATGATGGAAAAACAGTTGCAGCATTTGATATGGTTGTTCCAAAGGCTGGGAGATTATTTTCTGGTAGCCAAAATGAGGAGCGCATCAACATTCTAACTGCAAGGATTAAGGAGTTGGGATTGGCAGCGCAGCAGTATGAATGGTACTTGGATCTTCGCCGGCATGGAACGGTTAAGCACTCGGGATTCAATCTTGAGTTTGATCATATCGTTCTGTTTGCTACTGGCCTGAATGATGTAAGGGATGTCATCCCCTTTCCCAGAAGCCATGGCAAAGCCAACTACTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004812 GO:0004816 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0006082 GO:0006139 GO:0006399 GO:0006412 GO:0006418 GO:0006421 GO:0006518 GO:0006520 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009507 GO:0009536 GO:0009987 GO:0010467 GO:0016070 GO:0016874 GO:0016875 GO:0019538 GO:0019752 GO:0034641 GO:0034645 GO:0034660 GO:0043038 GO:0043039 GO:0043043 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044281 GO:0044424 GO:0044444 GO:0044464 GO:0046483 GO:0071704 GO:0090304 GO:0140098 GO:0140101 GO:1901360 GO:1901564 GO:1901566 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00970 map00970

Pfam Domains

Protein Families

tRNA-synt_2

Protein Analysis

625

Amino Acids

69.62

Weight (kDa)

8.34

Isoelectric Point (pI)

43.43

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
tRNA-synt_2	PF00152	357 - 618	3e-38	tRNA synthetases class II (D, K and N)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0010957)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G07420 AT3G07420
fragaria_vesca	FvH4_2g17960
malus_domestica	MD05G1163500.v1.1 MD10G1153300.v1.1
prunus_persica	Prupe.8G192100_v2.0.a1
pyrus_communis	pycom05g15290 pycom10g13220
rosa_chinensis	RchiOBHm_Chr6g0283201
rosa_laevigata	RLG00000012837
rosa_multiflora	Rmu_sc0006459.1_g000001 Rmu_sc0013038.1_g000019
rosa_roxburghii	Rroxscaffold_2G00106680 Rroxscaffold_7G00185650
rosa_rugosa	Rorug06G0152600
rosa_samantha	Rh6AG265000 Rh6BG267900 Rh6CG267100 Rh6DG260300
rosa_wichuraiana	Rw6G022970

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	755
AarI	CACCTGC	1 cut(s)	809
AatII	GACGTC	1 cut(s)	237
Acc36I	ACCTGC	1 cut(s)	809
AccB7I	CCANNNNNTGG	1 cut(s)	1634
AccBSI	CCGCTC	1 cut(s)	704
AciI	CCGC	7 cut(s)	20, 344, 362, 649, 682, 702, 1098
AclWI	GGATC	4 cut(s)	555, 771, 784, 1746
AcoI	YGGCCR	1 cut(s)	1105
AcsI	RAATTY	3 cut(s)	249, 629, 1328
AcuI	CTGAAG	4 cut(s)	239, 401, 1355, 1509
AcyI	GRCGYC	1 cut(s)	234
AfaI	GTAC	6 cut(s)	34, 59, 513, 747, 1151, 1733
AfiI	CCNNNNNNNGG	3 cut(s)	225, 1634, 1663
AflII	CTTAAG	1 cut(s)	326
AflIII	ACRYGT	2 cut(s)	526, 604
AjnI	CCWGG	2 cut(s)	438, 448
AjuI	GAANNNNNNNTTGG	2 cut(s)	71, 103
AluBI	AGCT	7 cut(s)	29, 240, 457, 538, 845, 956, 1125
AluI	AGCT	7 cut(s)	29, 240, 457, 538, 845, 956, 1125
Alw21I	GWGCWC	1 cut(s)	75
Alw26I	GTCTC	2 cut(s)	264, 1451
AlwI	GGATC	4 cut(s)	555, 771, 784, 1746
AlwNI	CAGNNNCTG	1 cut(s)	956
Ama87I	CYCGRG	1 cut(s)	1768
AoxI	GGCC	5 cut(s)	451, 1105, 1160, 1233, 1813
ApoI	RAATTY	3 cut(s)	249, 629, 1328
Asp700I	GAANNNNTTC	1 cut(s)	252
AspLEI	GCGC	3 cut(s)	349, 1671, 1717
AspS9I	GGNCC	1 cut(s)	1160
AsuC2I	CCSGG	1 cut(s)	210
AsuHPI	GGTGA	6 cut(s)	134, 160, 209, 224, 802, 833
AvaI	CYCGRG	1 cut(s)	1768
BaeGI	GKGCMC	1 cut(s)	1138
BamHI	GGATCC	1 cut(s)	776
BanII	GRGCYC	1 cut(s)	50
BbsI	GAAGAC	1 cut(s)	370
Bbv12I	GWGCWC	1 cut(s)	75
BccI	CCATC	7 cut(s)	92, 95, 188, 389, 564, 854, 1586
BceAI	ACGGC	1 cut(s)	784
BcgI	CGANNNNNNTGC	2 cut(s)	52, 86
BciT130I	CCWGG	2 cut(s)	440, 450
BciVI	GTATCC	2 cut(s)	258, 597
BclI	TGATCA	2 cut(s)	199, 1789
BcnI	CCSGG	1 cut(s)	210
BcoDI	GTCTC	2 cut(s)	264, 1451
BfaI	CTAG	7 cut(s)	30, 614, 801, 872, 914, 1080, 1873
BfmI	CTRYAG	6 cut(s)	497, 515, 762, 888, 1203, 1408
BfoI	RGCGCY	1 cut(s)	350
BfrI	CTTAAG	1 cut(s)	326
BfuAI	ACCTGC	1 cut(s)	809
BfuI	GTATCC	2 cut(s)	258, 597
BglII	AGATCT	1 cut(s)	1495
BmcAI	AGTACT	1 cut(s)	59
Bme1390I	CCNGG	3 cut(s)	210, 440, 450
BmeT110I	CYCGRG	1 cut(s)	1768
BmgT120I	GGNCC	1 cut(s)	1160
BmiI	GGNNCC	3 cut(s)	444, 778, 834
BmrFI	CCNGG	3 cut(s)	210, 440, 450
BmsI	GCATC	6 cut(s)	1004, 1101, 1123, 1206, 1414, 1680
BpiI	GAAGAC	1 cut(s)	370
BpmI	CTGGAG	1 cut(s)	1139
BpuEI	CTTGAG	2 cut(s)	908, 1802
BpuMI	CCSGG	1 cut(s)	210
BsaAI	YACGTR	2 cut(s)	188, 585
BsaBI	GATNNNNATC	1 cut(s)	198
BsaHI	GRCGYC	1 cut(s)	234
BsaJI	CCNNGG	7 cut(s)	159, 224, 438, 448, 690, 1138, 1855
BsaWI	WCCGGW	1 cut(s)	1578
BsaXI	ACNNNNNCTCC	2 cut(s)	132, 162
Bsc4I	CCNNNNNNNGG	3 cut(s)	225, 1634, 1663
Bse118I	RCCGGY	1 cut(s)	1746
Bse1I	ACTGG	3 cut(s)	472, 1008, 1816
Bse3DI	GCAATG	2 cut(s)	691, 969
Bse8I	GATNNNNATC	1 cut(s)	198
BseBI	CCWGG	2 cut(s)	440, 450
BseDI	CCNNGG	7 cut(s)	159, 224, 438, 448, 690, 1138, 1855
BseGI	GGATG	6 cut(s)	106, 400, 1116, 1221, 1836, 1837
BseJI	GATNNNNATC	1 cut(s)	198
BseLI	CCNNNNNNNGG	3 cut(s)	225, 1634, 1663
BseMI	GCAATG	2 cut(s)	691, 969
BseMII	CTCAG	2 cut(s)	1052, 1056
BseNI	ACTGG	3 cut(s)	472, 1008, 1816
BseRI	GAGGAG	3 cut(s)	315, 845, 1679
BseSI	GKGCMC	1 cut(s)	1138
BseYI	CCCAGC	1 cut(s)	1634
BshFI	GGCC	5 cut(s)	453, 1107, 1162, 1235, 1815
BsiHKAI	GWGCWC	1 cut(s)	75
BsiHKCI	CYCGRG	1 cut(s)	1768
BsiSI	CCGG	4 cut(s)	209, 1108, 1579, 1747
BslFI	GGGAC	1 cut(s)	1174
BslI	CCNNNNNNNGG	3 cut(s)	225, 1634, 1663
BsmAI	GTCTC	2 cut(s)	264, 1451
BsmFI	GGGAC	1 cut(s)	1174
BsnI	GGCC	5 cut(s)	453, 1107, 1162, 1235, 1815
BsoBI	CYCGRG	1 cut(s)	1768
Bsp1286I	GDGCHC	3 cut(s)	50, 75, 1138
Bsp143I	GATC	8 cut(s)	199, 547, 776, 1363, 1377, 1495, 1738, 1789
Bsp19I	CCATGG	1 cut(s)	1855
BspACI	CCGC	7 cut(s)	20, 344, 362, 649, 682, 702, 1098
BspANI	GGCC	5 cut(s)	453, 1107, 1162, 1235, 1815
BspCNI	CTCAG	2 cut(s)	1051, 1057
BspLI	GGNNCC	3 cut(s)	444, 778, 834
BspMAI	CTGCAG	3 cut(s)	501, 892, 1207
BspMI	ACCTGC	1 cut(s)	809
BspPI	GGATC	4 cut(s)	555, 771, 784, 1746
BspTI	CTTAAG	1 cut(s)	326
BsrBI	CCGCTC	1 cut(s)	704
BsrDI	GCAATG	2 cut(s)	691, 969
BsrFI	RCCGGY	1 cut(s)	1746
BsrI	ACTGG	3 cut(s)	472, 1008, 1816
BssAI	RCCGGY	1 cut(s)	1746
BssECI	CCNNGG	7 cut(s)	159, 224, 438, 448, 690, 1138, 1855
BssMI	GATC	8 cut(s)	199, 547, 776, 1363, 1377, 1495, 1738, 1789
BssNI	GRCGYC	1 cut(s)	234
BssT1I	CCWWGG	5 cut(s)	159, 224, 690, 1138, 1855
Bst2UI	CCWGG	2 cut(s)	440, 450
Bst4CI	ACNGT	5 cut(s)	376, 661, 1412, 1603, 1759
BstACI	GRCGYC	1 cut(s)	234
BstAFI	CTTAAG	1 cut(s)	326
BstAPI	GCANNNNNTGC	2 cut(s)	956, 1424
BstBAI	YACGTR	2 cut(s)	188, 585
BstC8I	GCNNGC	4 cut(s)	412, 455, 494, 1748
BstDEI	CTNAG	5 cut(s)	623, 837, 1038, 1065, 1499
BstDSI	CCRYGG	1 cut(s)	1855
BstF5I	GGATG	6 cut(s)	106, 400, 1116, 1221, 1836, 1837
BstH2I	RGCGCY	1 cut(s)	350
BstHHI	GCGC	3 cut(s)	349, 1671, 1717
BstKTI	GATC	8 cut(s)	202, 550, 779, 1366, 1380, 1498, 1741, 1792
BstMAI	GTCTC	2 cut(s)	264, 1451
BstMBI	GATC	8 cut(s)	199, 547, 776, 1363, 1377, 1495, 1738, 1789
BstMWI	GCNNNNNNNGC	5 cut(s)	26, 355, 411, 956, 1424
BstNI	CCWGG	2 cut(s)	440, 450
BstNSI	RCATGY	2 cut(s)	530, 1202
BstSCI	CCNGG	3 cut(s)	208, 438, 448
BstSFI	CTRYAG	6 cut(s)	497, 515, 762, 888, 1203, 1408
BstSLI	GKGCMC	1 cut(s)	1138
BstV2I	GAAGAC	1 cut(s)	370
BstX2I	RGATCY	3 cut(s)	776, 1495, 1738
BstYI	RGATCY	3 cut(s)	776, 1495, 1738
BsuI	GTATCC	2 cut(s)	258, 597
BsuRI	GGCC	5 cut(s)	453, 1107, 1162, 1235, 1815
BtgI	CCRYGG	1 cut(s)	1855
BtsCI	GGATG	6 cut(s)	106, 400, 1116, 1221, 1836, 1837
BtsIMutI	CAGTG	2 cut(s)	372, 1417
BveI	ACCTGC	1 cut(s)	809
Cac8I	GCNNGC	4 cut(s)	412, 455, 494, 1748
CaiI	CAGNNNCTG	1 cut(s)	956
CfoI	GCGC	3 cut(s)	349, 1671, 1717
Cfr10I	RCCGGY	1 cut(s)	1746
Cfr13I	GGNCC	1 cut(s)	1160
CseI	GACGC	2 cut(s)	232, 466
Csp6I	GTAC	6 cut(s)	33, 58, 512, 746, 1150, 1732
CviAII	CATG	7 cut(s)	203, 527, 551, 794, 1199, 1751, 1856
CviQI	GTAC	6 cut(s)	33, 58, 512, 746, 1150, 1732
DdeI	CTNAG	5 cut(s)	623, 837, 1038, 1065, 1499
DpnI	GATC	8 cut(s)	201, 549, 778, 1365, 1379, 1497, 1740, 1791
DpnII	GATC	8 cut(s)	199, 547, 776, 1363, 1377, 1495, 1738, 1789
EaeI	YGGCCR	1 cut(s)	1105
EciI	GGCGGA	1 cut(s)	351
Eco130I	CCWWGG	5 cut(s)	159, 224, 690, 1138, 1855
Eco24I	GRGCYC	1 cut(s)	50
Eco57I	CTGAAG	4 cut(s)	239, 401, 1355, 1509
Eco88I	CYCGRG	1 cut(s)	1768
EcoRII	CCWGG	2 cut(s)	438, 448
EcoT14I	CCWWGG	5 cut(s)	159, 224, 690, 1138, 1855
EcoT22I	ATGCAT	2 cut(s)	1116, 1429
EcoT38I	GRGCYC	1 cut(s)	50
ErhI	CCWWGG	5 cut(s)	159, 224, 690, 1138, 1855
FaeI	CATG	7 cut(s)	206, 530, 554, 797, 1202, 1754, 1859
FaqI	GGGAC	1 cut(s)	1174
FatI	CATG	7 cut(s)	202, 526, 550, 793, 1198, 1750, 1855
FbaI	TGATCA	2 cut(s)	199, 1789
FokI	GGATG	6 cut(s)	113, 407, 1123, 1228, 1823, 1844
FriOI	GRGCYC	1 cut(s)	50
FspBI	CTAG	7 cut(s)	30, 614, 801, 872, 914, 1080, 1873
GlaI	GCGC	3 cut(s)	348, 1670, 1716
GsaI	CCCAGC	1 cut(s)	1638
GsuI	CTGGAG	1 cut(s)	1139
HaeII	RGCGCY	1 cut(s)	350
HaeIII	GGCC	5 cut(s)	453, 1107, 1162, 1235, 1815
HapII	CCGG	4 cut(s)	209, 1108, 1579, 1747
HgaI	GACGC	2 cut(s)	232, 466
HhaI	GCGC	3 cut(s)	349, 1671, 1717
Hin1I	GRCGYC	1 cut(s)	234
Hin1II	CATG	7 cut(s)	206, 530, 554, 797, 1202, 1754, 1859
Hin6I	GCGC	3 cut(s)	347, 1669, 1715
HinP1I	GCGC	3 cut(s)	347, 1669, 1715
HincII	GTYRAC	1 cut(s)	1048
HindII	GTYRAC	1 cut(s)	1048
HinfI	GANTC	6 cut(s)	5, 431, 678, 1187, 1344, 1773
HpaII	CCGG	4 cut(s)	209, 1108, 1579, 1747
HphI	GGTGA	6 cut(s)	134, 160, 209, 224, 802, 833
Hpy166II	GTNNAC	3 cut(s)	215, 1048, 1150
Hpy188I	TCNGA	7 cut(s)	232, 264, 566, 1041, 1066, 1243, 1318
Hpy188III	TCNNGA	9 cut(s)	76, 169, 382, 614, 872, 925, 1118, 1770, 1781
Hpy8I	GTNNAC	3 cut(s)	215, 1048, 1150
Hpy99I	CGWCG	1 cut(s)	248
HpyAV	CCTTC	9 cut(s)	61, 90, 378, 425, 482, 516, 1036, 1379, 1429
HpyCH4III	ACNGT	5 cut(s)	376, 661, 1412, 1603, 1759
HpyCH4IV	ACGT	4 cut(s)	187, 234, 584, 604
HpyF10VI	GCNNNNNNNGC	5 cut(s)	26, 355, 411, 956, 1424
HpyF3I	CTNAG	5 cut(s)	623, 837, 1038, 1065, 1499
HpySE526I	ACGT	4 cut(s)	187, 234, 584, 604
Hsp92I	GRCGYC	1 cut(s)	234
Hsp92II	CATG	7 cut(s)	206, 530, 554, 797, 1202, 1754, 1859
HspAI	GCGC	3 cut(s)	347, 1669, 1715
KroI	GCCGGC	1 cut(s)	1746
KroNI	GCCGGC	1 cut(s)	1748
Ksp22I	TGATCA	2 cut(s)	199, 1789
Kzo9I	GATC	8 cut(s)	199, 547, 776, 1363, 1377, 1495, 1738, 1789
LmnI	GCTCC	3 cut(s)	448, 832, 1666
LweI	GCATC	6 cut(s)	1004, 1101, 1123, 1206, 1414, 1680
MaeI	CTAG	7 cut(s)	30, 614, 801, 872, 914, 1080, 1873
MaeII	ACGT	4 cut(s)	187, 234, 584, 604
MaeIII	GTNAC	4 cut(s)	796, 905, 1070, 1438
MalI	GATC	8 cut(s)	201, 549, 778, 1365, 1379, 1497, 1740, 1791
MbiI	CCGCTC	1 cut(s)	704
MboI	GATC	8 cut(s)	199, 547, 776, 1363, 1377, 1495, 1738, 1789
MboII	GAAGA	5 cut(s)	245, 370, 1053, 1331, 1733
MfeI	CAATTG	2 cut(s)	282, 1261
MflI	RGATCY	3 cut(s)	776, 1495, 1738
MhlI	GDGCHC	3 cut(s)	50, 75, 1138
MlyI	GAGTC	1 cut(s)	1196
MmeI	TCCRAC	1 cut(s)	318
Mph1103I	ATGCAT	2 cut(s)	1116, 1429
MroNI	GCCGGC	1 cut(s)	1746
MroXI	GAANNNNTTC	1 cut(s)	252
MseI	TTAA	7 cut(s)	116, 327, 974, 1056, 1430, 1695, 1761
MspA1I	CMGCKG	3 cut(s)	20, 240, 649
MspCI	CTTAAG	1 cut(s)	326
MspI	CCGG	4 cut(s)	209, 1108, 1579, 1747
MspR9I	CCNGG	3 cut(s)	210, 440, 450
MunI	CAATTG	2 cut(s)	282, 1261
MvaI	CCWGG	2 cut(s)	440, 450
MwoI	GCNNNNNNNGC	5 cut(s)	26, 355, 411, 956, 1424
NaeI	GCCGGC	1 cut(s)	1748
NciI	CCSGG	1 cut(s)	210
NcoI	CCATGG	1 cut(s)	1855
NdeII	GATC	8 cut(s)	199, 547, 776, 1363, 1377, 1495, 1738, 1789
NgoMIV	GCCGGC	1 cut(s)	1746
NlaIII	CATG	7 cut(s)	206, 530, 554, 797, 1202, 1754, 1859
NlaIV	GGNNCC	3 cut(s)	444, 778, 834
NmuCI	GTSAC	1 cut(s)	796
NsiI	ATGCAT	2 cut(s)	1116, 1429
NspI	RCATGY	2 cut(s)	530, 1202
PaqCI	CACCTGC	1 cut(s)	809
PciI	ACATGT	1 cut(s)	526
PcsI	WCGNNNNNNNCGW	1 cut(s)	240
PdiI	GCCGGC	1 cut(s)	1748
PdmI	GAANNNNTTC	1 cut(s)	252
PfeI	GAWTC	5 cut(s)	5, 431, 678, 1344, 1773
PflMI	CCANNNNNTGG	1 cut(s)	1634
PleI	GAGTC	1 cut(s)	1195
PpsI	GAGTC	1 cut(s)	1195
Ppu21I	YACGTR	2 cut(s)	188, 585
PscI	ACATGT	1 cut(s)	526
PsiI	TTATAA	1 cut(s)	755
Psp6I	CCWGG	2 cut(s)	438, 448
PspFI	CCCAGC	1 cut(s)	1634
PspGI	CCWGG	2 cut(s)	438, 448
PspN4I	GGNNCC	3 cut(s)	444, 778, 834
PspPI	GGNCC	1 cut(s)	1160
PstI	CTGCAG	3 cut(s)	501, 892, 1207
PstNI	CAGNNNCTG	1 cut(s)	956
PsuI	RGATCY	3 cut(s)	776, 1495, 1738
PvuII	CAGCTG	1 cut(s)	240
RsaI	GTAC	6 cut(s)	34, 59, 513, 747, 1151, 1733
RsaNI	GTAC	6 cut(s)	33, 58, 512, 746, 1150, 1732
SaqAI	TTAA	7 cut(s)	116, 327, 974, 1056, 1430, 1695, 1761
Sau3AI	GATC	8 cut(s)	199, 547, 776, 1363, 1377, 1495, 1738, 1789
Sau96I	GGNCC	1 cut(s)	1160
ScaI	AGTACT	1 cut(s)	59
SchI	GAGTC	1 cut(s)	1196
ScrFI	CCNGG	3 cut(s)	210, 440, 450
SduI	GDGCHC	3 cut(s)	50, 75, 1138
SfaNI	GCATC	6 cut(s)	1004, 1101, 1123, 1206, 1414, 1680
SfcI	CTRYAG	6 cut(s)	497, 515, 762, 888, 1203, 1408
SmlI	CTYRAG	3 cut(s)	326, 923, 1781
SmoI	CTYRAG	3 cut(s)	326, 923, 1781
SsiI	CCGC	7 cut(s)	20, 344, 362, 649, 682, 702, 1098
SspMI	CTAG	7 cut(s)	30, 614, 801, 872, 914, 1080, 1873
StyD4I	CCNGG	3 cut(s)	208, 438, 448
StyI	CCWWGG	5 cut(s)	159, 224, 690, 1138, 1855
TaaI	ACNGT	5 cut(s)	376, 661, 1412, 1603, 1759
TaiI	ACGT	4 cut(s)	190, 237, 587, 607
TaqI	TCGA	6 cut(s)	62, 246, 534, 1171, 1347, 1376
TatI	WGTACW	2 cut(s)	57, 1149
TauI	GCSGC	3 cut(s)	23, 347, 704
TfiI	GAWTC	5 cut(s)	5, 431, 678, 1344, 1773
Tru1I	TTAA	7 cut(s)	116, 327, 974, 1056, 1430, 1695, 1761
Tru9I	TTAA	7 cut(s)	116, 327, 974, 1056, 1430, 1695, 1761
TscAI	CASTG	2 cut(s)	379, 1417
TseFI	GTSAC	1 cut(s)	796
Tsp45I	GTSAC	1 cut(s)	796
TspDTI	ATGAA	4 cut(s)	341, 1340, 1380, 1740
TspRI	CASTG	2 cut(s)	379, 1417
Van91I	CCANNNNNTGG	1 cut(s)	1634
Vha464I	CTTAAG	1 cut(s)	326
XapI	RAATTY	3 cut(s)	249, 629, 1328
XbaI	TCTAGA	2 cut(s)	613, 871
XceI	RCATGY	2 cut(s)	530, 1202
XmnI	GAANNNNTTC	1 cut(s)	252
XspI	CTAG	7 cut(s)	30, 614, 801, 872, 914, 1080, 1873
ZraI	GACGTC	1 cut(s)	235
ZrmI	AGTACT	1 cut(s)	59
Zsp2I	ATGCAT	2 cut(s)	1116, 1429

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.