Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr10

Orthogroup ID

OG0010957

Physical Location & Seq

Genomic Coordinates Forward (+)

24081405 .. 24084235

Gene Structure 2831 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD10G1153300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1683 bp

ATGGAATCTGAACAAGCCGCGACAGCTGCTAGTACCACCCGATTGAGCCCTTTCAAGTACTCGAATCGGGTGCTTTTGAAAACCATCTTGGATAGAAGGGATGGTGGTTTGGAGTTGATTGGTGAGAGGGTTGTGATTGGAGGATGGGTGAAGTCCTCCAAGGAGGTCAGGAAAGAGCCGGTGCCACATATTGCTGGTGATTATGTGTCGGGTGAACCTGAACCAAGGTCAGACGTCTGCTGCTGCATCGAAATTCTTCAGTCTCGGATTCCTTTTTTACGAACAATTGTTAAGGTCTTGGGAGGAGGAAGTGGCGGTCATTATCCACTTACGGATAAGTTGGAAACATCGATTCATACGTCCCCGCCGCCTTCCACTGTCGTCTTGCAAGTCGGCGATGGTTCTTGCGTTGCAAACCTTCAGGTTGTTATTGAATCCTCCCTGACTCCCCCATGCCAGCTCTTGCATACTGGAACTTGCATCATAGTGGAAGGCTTGCTGCAGAAGTCTTGGGTACTACAAGGCAAATACGTTATCGAGCTGAAAGTGGATAAACTTCATCACATCGGAACAGTAGAATATAGCAGTTATCCATTATCGAAGAAACGCGTGCCTCTAGATAAACTAAGGGAGTTTTGCCACATTAGACCGCGGACAACTACGGTGCAATCCGTCATGCGAATCCGCAATGCCCTGGAGTTTGCAGCTCACACATTTTGTCAAAACCATGGTTTTCTTTCGGTGCATGTACCCATTATAACAACTACAGACGGCAAAGGATCCAGTGAAAAATTCCATGTCACTAGCCATTTCGCAGCAGCAGGTGAAAAGGAGGAGCCTAAGAAAGCCGTTGTTGACAATGTTAAATCTTCTGAGGGTTACTTCTCTAGCCCAACTTATCTAACCTCTTCTGGCCGGATGCATCTGGAGAGCTACGCTTGCGCCCTTGGAAATGTGTACTCATTCGGGCCACGATTTCGAGCAGAGAAAGGAGAGTCCCCAAAACATGCTGCAGAAATGTGGATAATTGAAGTTGAATTGGCATTTTCAGAATTAGAGGAAGCAATCAACTGTGCAGGTGACTTTTTCAAGTCCCTCTGCAAATGGGTAGTGGAAAATTGTTCGGAAGATATGAAATTCCTTTCTCAGCGAATCGACAAAACCAGAACCGAACGTCTTCAGTCAATGGTTTTGAGTACATTTGAAAGAGTTTCCTACACTGAGGCTTTGGATGCTTTAAAGAAGGTTACAGAAAAGAAAAAGGACACAATGATCGAGTGGGGTGCTGCACTAACTGAAGCGAATCTAAGTTATTTGGCTGATGAAATCTACAAGAAGCCTGTAATTGTATACAATTATCCAAAAGAAACCAAGCCATTTTATGTCCGGCTTAACGATGATGGAAAAACAGTTGCAGCATTTGATATGGTTGTTCCAAAGGCTGGGAGGTTAATTTCTGGTAGCCAAAATGAGGAGCGCATCAACATTCTAACTACAAGGATTAAAGAATTGGGATTGGCAGCACAGCAGTACGAATGGTACTTGGATCTTCGCCGGCATGGAACCGTGAAGCACTCGGGATTCAATCTTGAGTTTGATCATATGGTTCTGTTTGCCACTGGCCTGAATGATGTAAGGGATGTCATCCCCTTTCCCAGAAGCCGTGGCAAAGCTAGCTACTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004812 GO:0004816 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0006082 GO:0006139 GO:0006399 GO:0006412 GO:0006418 GO:0006421 GO:0006518 GO:0006520 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009507 GO:0009536 GO:0009987 GO:0010467 GO:0016070 GO:0016874 GO:0016875 GO:0019538 GO:0019752 GO:0034641 GO:0034645 GO:0034660 GO:0043038 GO:0043039 GO:0043043 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044281 GO:0044424 GO:0044444 GO:0044464 GO:0046483 GO:0071704 GO:0090304 GO:0140098 GO:0140101 GO:1901360 GO:1901564 GO:1901566 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00970 map00970

Pfam Domains

Protein Families

tRNA-synt_2

Protein Analysis

561

Amino Acids

62.65

Weight (kDa)

8.13

Isoelectric Point (pI)

46.86

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
tRNA-synt_2	PF00152	214 - 554	2.2e-45	tRNA synthetases class II (D, K and N)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0010957)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G07420 AT3G07420
fragaria_vesca	FvH4_2g17960
malus_domestica	MD05G1163500.v1.1 MD10G1153300.v1.1
prunus_persica	Prupe.8G192100_v2.0.a1
pyrus_communis	pycom05g15290 pycom10g13220
rosa_chinensis	RchiOBHm_Chr6g0283201
rosa_laevigata	RLG00000012837
rosa_multiflora	Rmu_sc0006459.1_g000001 Rmu_sc0013038.1_g000019
rosa_roxburghii	Rroxscaffold_2G00106680 Rroxscaffold_7G00185650
rosa_rugosa	Rorug06G0152600
rosa_samantha	Rh6AG265000 Rh6BG267900 Rh6CG267100 Rh6DG260300
rosa_wichuraiana	Rw6G022970

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	758
AarI	CACCTGC	2 cut(s)	812, 1067
AatII	GACGTC	1 cut(s)	237
Acc36I	ACCTGC	2 cut(s)	812, 1067
AccB1I	GGYRCC	1 cut(s)	181
AccB7I	CCANNNNNTGG	1 cut(s)	1442
AccI	GTMKAC	1 cut(s)	1350
AccII	CGCG	3 cut(s)	20, 609, 652
AciI	CCGC	7 cut(s)	18, 315, 365, 368, 650, 652, 685
AclWI	GGATC	3 cut(s)	774, 787, 1554
AcoI	YGGCCR	1 cut(s)	913
AcsI	RAATTY	3 cut(s)	252, 791, 1136
AcuI	CTGAAG	4 cut(s)	242, 404, 1163, 1317
AcyI	GRCGYC	1 cut(s)	234
AfaI	GTAC	8 cut(s)	34, 59, 516, 750, 959, 1198, 1532, 1541
AfiI	CCNNNNNNNGG	2 cut(s)	1442, 1471
AflIII	ACRYGT	1 cut(s)	607
AgsI	TTSAA	8 cut(s)	55, 79, 434, 1031, 1037, 1090, 1205, 1585
AjnI	CCWGG	1 cut(s)	693
AjuI	GAANNNNNNNTTGG	2 cut(s)	71, 103
AluBI	AGCT	7 cut(s)	26, 460, 541, 707, 933, 1673, 1677
AluI	AGCT	7 cut(s)	26, 460, 541, 707, 933, 1673, 1677
Alw26I	GTCTC	1 cut(s)	267
AlwI	GGATC	3 cut(s)	774, 787, 1554
Ama87I	CYCGRG	1 cut(s)	1576
AoxI	GGCC	3 cut(s)	913, 968, 1621
ApoI	RAATTY	3 cut(s)	252, 791, 1136
Asp700I	GAANNNNTTC	2 cut(s)	255, 1176
AspLEI	GCGC	2 cut(s)	944, 1479
AspS9I	GGNCC	1 cut(s)	968
AsuHPI	GGTGA	6 cut(s)	134, 160, 209, 224, 836, 1091
AsuNHI	GCTAGC	1 cut(s)	1673
AvaI	CYCGRG	1 cut(s)	1576
BamHI	GGATCC	1 cut(s)	779
BanI	GGYRCC	1 cut(s)	181
BanII	GRGCYC	1 cut(s)	50
BbsI	GAAGAC	1 cut(s)	1169
BccI	CCATC	5 cut(s)	92, 95, 138, 392, 1394
BceAI	ACGGC	3 cut(s)	787, 833, 1647
BcgI	CGANNNNNNTGC	4 cut(s)	52, 86, 1265, 1299
BciT130I	CCWGG	1 cut(s)	695
BclI	TGATCA	1 cut(s)	1597
BcoDI	GTCTC	1 cut(s)	267
BfaI	CTAG	6 cut(s)	30, 617, 804, 888, 1674, 1681
BfmI	CTRYAG	3 cut(s)	500, 765, 1011
BfuAI	ACCTGC	2 cut(s)	812, 1067
BmcAI	AGTACT	1 cut(s)	59
Bme1390I	CCNGG	1 cut(s)	695
BmeT110I	CYCGRG	1 cut(s)	1576
BmgT120I	GGNCC	1 cut(s)	968
BmiI	GGNNCC	4 cut(s)	183, 781, 837, 1564
BmrFI	CCNGG	1 cut(s)	695
BmsI	GCATC	6 cut(s)	255, 489, 909, 931, 1222, 1488
BmtI	GCTAGC	1 cut(s)	1677
BpiI	GAAGAC	1 cut(s)	1169
BpmI	CTGGAG	2 cut(s)	716, 947
BpuEI	CTTGAG	1 cut(s)	1610
Bsa29I	ATCGAT	1 cut(s)	350
BsaHI	GRCGYC	1 cut(s)	234
BsaJI	CCNNGG	7 cut(s)	159, 224, 650, 693, 727, 946, 1663
BsaXI	ACNNNNNCTCC	2 cut(s)	132, 162
Bsc4I	CCNNNNNNNGG	2 cut(s)	1442, 1471
Bse118I	RCCGGY	2 cut(s)	178, 1554
Bse1I	ACTGG	3 cut(s)	475, 783, 1624
Bse3DI	GCAATG	1 cut(s)	694
BseBI	CCWGG	1 cut(s)	695
BseCI	ATCGAT	1 cut(s)	350
BseDI	CCNNGG	7 cut(s)	159, 224, 650, 693, 727, 946, 1663
BseGI	GGATG	6 cut(s)	106, 149, 924, 1237, 1644, 1645
BseLI	CCNNNNNNNGG	2 cut(s)	1442, 1471
BseMI	GCAATG	1 cut(s)	694
BseMII	CTCAG	3 cut(s)	864, 1160, 1212
BseNI	ACTGG	3 cut(s)	475, 783, 1624
BseRI	GAGGAG	3 cut(s)	318, 848, 1487
BseYI	CCCAGC	1 cut(s)	1442
BsgI	GTGCAG	2 cut(s)	1095, 1272
Bsh1236I	CGCG	3 cut(s)	20, 609, 652
BshFI	GGCC	3 cut(s)	915, 970, 1623
BshNI	GGYRCC	1 cut(s)	181
BshVI	ATCGAT	1 cut(s)	350
BsiHKCI	CYCGRG	1 cut(s)	1576
BsiSI	CCGG	4 cut(s)	179, 916, 1387, 1555
BslFI	GGGAC	3 cut(s)	346, 982, 1078
BslI	CCNNNNNNNGG	2 cut(s)	1442, 1471
BsmAI	GTCTC	1 cut(s)	267
BsmFI	GGGAC	3 cut(s)	346, 982, 1078
BsnI	GGCC	3 cut(s)	915, 970, 1623
BsoBI	CYCGRG	1 cut(s)	1576
Bsp1286I	GDGCHC	1 cut(s)	50
Bsp143I	GATC	4 cut(s)	779, 1272, 1546, 1597
Bsp19I	CCATGG	1 cut(s)	727
BspACI	CCGC	7 cut(s)	18, 315, 365, 368, 650, 652, 685
BspANI	GGCC	3 cut(s)	915, 970, 1623
BspCNI	CTCAG	3 cut(s)	865, 1159, 1213
BspDI	ATCGAT	1 cut(s)	350
BspFNI	CGCG	3 cut(s)	20, 609, 652
BspLI	GGNNCC	4 cut(s)	183, 781, 837, 1564
BspMAI	CTGCAG	2 cut(s)	504, 1015
BspMI	ACCTGC	2 cut(s)	812, 1067
BspOI	GCTAGC	1 cut(s)	1677
BspPI	GGATC	3 cut(s)	774, 787, 1554
BspT107I	GGYRCC	1 cut(s)	181
BsrDI	GCAATG	1 cut(s)	694
BsrFI	RCCGGY	2 cut(s)	178, 1554
BsrI	ACTGG	3 cut(s)	475, 783, 1624
BssAI	RCCGGY	2 cut(s)	178, 1554
BssECI	CCNNGG	7 cut(s)	159, 224, 650, 693, 727, 946, 1663
BssMI	GATC	4 cut(s)	779, 1272, 1546, 1597
BssNAI	GTATAC	1 cut(s)	1351
BssNI	GRCGYC	1 cut(s)	234
BssT1I	CCWWGG	4 cut(s)	159, 224, 727, 946
Bst1107I	GTATAC	1 cut(s)	1351
Bst2UI	CCWGG	1 cut(s)	695
Bst4CI	ACNGT	6 cut(s)	379, 574, 664, 1073, 1411, 1567
Bst6I	CTCTTC	1 cut(s)	913
BstACI	GRCGYC	1 cut(s)	234
BstC8I	GCNNGC	6 cut(s)	458, 497, 611, 940, 1556, 1675
BstDEI	CTNAG	6 cut(s)	626, 840, 873, 1146, 1221, 1307
BstDSI	CCRYGG	3 cut(s)	650, 727, 1663
BstF5I	GGATG	6 cut(s)	106, 149, 924, 1237, 1644, 1645
BstFNI	CGCG	3 cut(s)	20, 609, 652
BstHHI	GCGC	2 cut(s)	944, 1479
BstKTI	GATC	4 cut(s)	782, 1275, 1549, 1600
BstMAI	GTCTC	1 cut(s)	267
BstMBI	GATC	4 cut(s)	779, 1272, 1546, 1597
BstMWI	GCNNNNNNNGC	5 cut(s)	23, 26, 939, 1232, 1674
BstNI	CCWGG	1 cut(s)	695
BstNSI	RCATGY	2 cut(s)	749, 1010
BstSCI	CCNGG	1 cut(s)	693
BstSFI	CTRYAG	3 cut(s)	500, 765, 1011
BstUI	CGCG	3 cut(s)	20, 609, 652
BstV2I	GAAGAC	1 cut(s)	1169
BstX2I	RGATCY	2 cut(s)	779, 1546
BstYI	RGATCY	2 cut(s)	779, 1546
BstZ17I	GTATAC	1 cut(s)	1351
Bsu15I	ATCGAT	1 cut(s)	350
BsuRI	GGCC	3 cut(s)	915, 970, 1623
BsuTUI	ATCGAT	1 cut(s)	350
BtgI	CCRYGG	3 cut(s)	650, 727, 1663
BtgZI	GCGATG	1 cut(s)	411
BtsCI	GGATG	6 cut(s)	106, 149, 924, 1237, 1644, 1645
BtsIMutI	CAGTG	4 cut(s)	375, 790, 1218, 1617
BveI	ACCTGC	2 cut(s)	812, 1067
Cac8I	GCNNGC	6 cut(s)	458, 497, 611, 940, 1556, 1675
CfoI	GCGC	2 cut(s)	944, 1479
Cfr10I	RCCGGY	2 cut(s)	178, 1554
Cfr13I	GGNCC	1 cut(s)	968
Cfr42I	CCGCGG	1 cut(s)	653
ClaI	ATCGAT	1 cut(s)	350
Csp6I	GTAC	8 cut(s)	33, 58, 515, 749, 958, 1197, 1531, 1540
CviAII	CATG	7 cut(s)	453, 676, 728, 746, 797, 1007, 1559
CviQI	GTAC	8 cut(s)	33, 58, 515, 749, 958, 1197, 1531, 1540
DdeI	CTNAG	6 cut(s)	626, 840, 873, 1146, 1221, 1307
DpnI	GATC	4 cut(s)	781, 1274, 1548, 1599
DpnII	GATC	4 cut(s)	779, 1272, 1546, 1597
DraI	TTTAAA	1 cut(s)	1239
EaeI	YGGCCR	1 cut(s)	913
Eam1104I	CTCTTC	1 cut(s)	913
EarI	CTCTTC	1 cut(s)	913
Eco130I	CCWWGG	4 cut(s)	159, 224, 727, 946
Eco24I	GRGCYC	1 cut(s)	50
Eco57I	CTGAAG	4 cut(s)	242, 404, 1163, 1317
Eco88I	CYCGRG	1 cut(s)	1576
EcoRII	CCWGG	1 cut(s)	693
EcoT14I	CCWWGG	4 cut(s)	159, 224, 727, 946
EcoT22I	ATGCAT	1 cut(s)	924
EcoT38I	GRGCYC	1 cut(s)	50
ErhI	CCWWGG	4 cut(s)	159, 224, 727, 946
FaeI	CATG	7 cut(s)	456, 679, 731, 749, 800, 1010, 1562
FaqI	GGGAC	3 cut(s)	346, 982, 1078
FatI	CATG	7 cut(s)	452, 675, 727, 745, 796, 1006, 1558
FauI	CCCGC	1 cut(s)	372
FauNDI	CATATG	1 cut(s)	1602
FbaI	TGATCA	1 cut(s)	1597
FblI	GTMKAC	1 cut(s)	1350
FokI	GGATG	6 cut(s)	113, 156, 931, 1244, 1631, 1652
FriOI	GRGCYC	1 cut(s)	50
FspBI	CTAG	6 cut(s)	30, 617, 804, 888, 1674, 1681
GlaI	GCGC	2 cut(s)	943, 1478
GsaI	CCCAGC	1 cut(s)	1446
GsuI	CTGGAG	2 cut(s)	716, 947
HaeIII	GGCC	3 cut(s)	915, 970, 1623
HapII	CCGG	4 cut(s)	179, 916, 1387, 1555
HhaI	GCGC	2 cut(s)	944, 1479
Hin1I	GRCGYC	1 cut(s)	234
Hin1II	CATG	7 cut(s)	456, 679, 731, 749, 800, 1010, 1562
Hin6I	GCGC	2 cut(s)	942, 1477
HinP1I	GCGC	2 cut(s)	942, 1477
HincII	GTYRAC	1 cut(s)	856
HindII	GTYRAC	1 cut(s)	856
HpaII	CCGG	4 cut(s)	179, 916, 1387, 1555
HphI	GGTGA	6 cut(s)	134, 160, 209, 224, 836, 1091
Hpy166II	GTNNAC	4 cut(s)	215, 856, 958, 1351
Hpy188I	TCNGA	7 cut(s)	10, 232, 267, 569, 874, 1051, 1126
Hpy188III	TCNNGA	5 cut(s)	169, 617, 926, 1578, 1589
Hpy8I	GTNNAC	4 cut(s)	215, 856, 958, 1351
HpyAV	CCTTC	5 cut(s)	90, 381, 428, 485, 1237
HpyCH4III	ACNGT	6 cut(s)	379, 574, 664, 1073, 1411, 1567
HpyCH4IV	ACGT	4 cut(s)	234, 359, 531, 1174
HpyF10VI	GCNNNNNNNGC	5 cut(s)	23, 26, 939, 1232, 1674
HpyF3I	CTNAG	6 cut(s)	626, 840, 873, 1146, 1221, 1307
HpySE526I	ACGT	4 cut(s)	234, 359, 531, 1174
Hsp92I	GRCGYC	1 cut(s)	234
Hsp92II	CATG	7 cut(s)	456, 679, 731, 749, 800, 1010, 1562
HspAI	GCGC	2 cut(s)	942, 1477
KroI	GCCGGC	1 cut(s)	1554
KroNI	GCCGGC	1 cut(s)	1556
Ksp22I	TGATCA	1 cut(s)	1597
KspI	CCGCGG	1 cut(s)	653
Kzo9I	GATC	4 cut(s)	779, 1272, 1546, 1597
LmnI	GCTCC	2 cut(s)	835, 1474
LweI	GCATC	6 cut(s)	255, 489, 909, 931, 1222, 1488
MaeI	CTAG	6 cut(s)	30, 617, 804, 888, 1674, 1681
MaeII	ACGT	4 cut(s)	234, 359, 531, 1174
MaeIII	GTNAC	4 cut(s)	799, 878, 1079, 1246
MalI	GATC	4 cut(s)	781, 1274, 1548, 1599
MboI	GATC	4 cut(s)	779, 1272, 1546, 1597
MboII	GAAGA	7 cut(s)	248, 613, 861, 900, 1139, 1169, 1541
MfeI	CAATTG	1 cut(s)	285
MflI	RGATCY	2 cut(s)	779, 1546
MhlI	GDGCHC	1 cut(s)	50
MluI	ACGCGT	1 cut(s)	607
MlyI	GAGTC	2 cut(s)	439, 1004
MmeI	TCCRAC	1 cut(s)	321
Mph1103I	ATGCAT	1 cut(s)	924
MroNI	GCCGGC	1 cut(s)	1554
MroXI	GAANNNNTTC	2 cut(s)	255, 1176
MseI	TTAA	6 cut(s)	291, 864, 1238, 1392, 1451, 1503
MslI	CAYNNNNRTG	1 cut(s)	485
MspA1I	CMGCKG	2 cut(s)	26, 652
MspI	CCGG	4 cut(s)	179, 916, 1387, 1555
MspR9I	CCNGG	1 cut(s)	695
MunI	CAATTG	1 cut(s)	285
MvaI	CCWGG	1 cut(s)	695
MvnI	CGCG	3 cut(s)	20, 609, 652
MwoI	GCNNNNNNNGC	5 cut(s)	23, 26, 939, 1232, 1674
NaeI	GCCGGC	1 cut(s)	1556
NcoI	CCATGG	1 cut(s)	727
NdeI	CATATG	1 cut(s)	1602
NdeII	GATC	4 cut(s)	779, 1272, 1546, 1597
NgoMIV	GCCGGC	1 cut(s)	1554
NheI	GCTAGC	1 cut(s)	1673
NlaIII	CATG	7 cut(s)	456, 679, 731, 749, 800, 1010, 1562
NlaIV	GGNNCC	4 cut(s)	183, 781, 837, 1564
NmuCI	GTSAC	2 cut(s)	799, 1079
NsiI	ATGCAT	1 cut(s)	924
NspI	RCATGY	2 cut(s)	749, 1010
PaqCI	CACCTGC	2 cut(s)	812, 1067
PcsI	WCGNNNNNNNCGW	1 cut(s)	356
PdiI	GCCGGC	1 cut(s)	1556
PdmI	GAANNNNTTC	2 cut(s)	255, 1176
PfeI	GAWTC	9 cut(s)	5, 64, 268, 352, 434, 681, 1152, 1303, 1581
PflMI	CCANNNNNTGG	1 cut(s)	1442
PleI	GAGTC	2 cut(s)	439, 1003
PpsI	GAGTC	2 cut(s)	439, 1003
PsiI	TTATAA	1 cut(s)	758
Psp6I	CCWGG	1 cut(s)	693
PspFI	CCCAGC	1 cut(s)	1442
PspGI	CCWGG	1 cut(s)	693
PspN4I	GGNNCC	4 cut(s)	183, 781, 837, 1564
PspPI	GGNCC	1 cut(s)	968
PstI	CTGCAG	2 cut(s)	504, 1015
PsuI	RGATCY	2 cut(s)	779, 1546
PvuII	CAGCTG	1 cut(s)	26
RsaI	GTAC	8 cut(s)	34, 59, 516, 750, 959, 1198, 1532, 1541
RsaNI	GTAC	8 cut(s)	33, 58, 515, 749, 958, 1197, 1531, 1540
RseI	CAYNNNNRTG	1 cut(s)	485
SacII	CCGCGG	1 cut(s)	653
SaqAI	TTAA	6 cut(s)	291, 864, 1238, 1392, 1451, 1503
Sau3AI	GATC	4 cut(s)	779, 1272, 1546, 1597
Sau96I	GGNCC	1 cut(s)	968
ScaI	AGTACT	1 cut(s)	59
SchI	GAGTC	2 cut(s)	439, 1004
ScrFI	CCNGG	1 cut(s)	695
SduI	GDGCHC	1 cut(s)	50
SfaNI	GCATC	6 cut(s)	255, 489, 909, 931, 1222, 1488
SfcI	CTRYAG	3 cut(s)	500, 765, 1011
Sfr303I	CCGCGG	1 cut(s)	653
SgrBI	CCGCGG	1 cut(s)	653
SmiMI	CAYNNNNRTG	1 cut(s)	485
SmlI	CTYRAG	1 cut(s)	1589
SmoI	CTYRAG	1 cut(s)	1589
SsiI	CCGC	7 cut(s)	18, 315, 365, 368, 650, 652, 685
SspMI	CTAG	6 cut(s)	30, 617, 804, 888, 1674, 1681
StyD4I	CCNGG	1 cut(s)	693
StyI	CCWWGG	4 cut(s)	159, 224, 727, 946
TaaI	ACNGT	6 cut(s)	379, 574, 664, 1073, 1411, 1567
TaiI	ACGT	4 cut(s)	237, 362, 534, 1177
TaqI	TCGA	8 cut(s)	62, 249, 350, 537, 599, 979, 1155, 1275
TatI	WGTACW	3 cut(s)	57, 957, 1196
TauI	GCSGC	2 cut(s)	20, 370
TfiI	GAWTC	9 cut(s)	5, 64, 268, 352, 434, 681, 1152, 1303, 1581
Tru1I	TTAA	6 cut(s)	291, 864, 1238, 1392, 1451, 1503
Tru9I	TTAA	6 cut(s)	291, 864, 1238, 1392, 1451, 1503
TscAI	CASTG	4 cut(s)	382, 790, 1225, 1624
TseFI	GTSAC	2 cut(s)	799, 1079
Tsp45I	GTSAC	2 cut(s)	799, 1079
TspDTI	ATGAA	4 cut(s)	344, 548, 1148, 1338
TspGWI	ACGGA	2 cut(s)	347, 661
TspRI	CASTG	4 cut(s)	382, 790, 1225, 1624
Van91I	CCANNNNNTGG	1 cut(s)	1442
XapI	RAATTY	3 cut(s)	252, 791, 1136
XbaI	TCTAGA	1 cut(s)	616
XceI	RCATGY	2 cut(s)	749, 1010
XmiI	GTMKAC	1 cut(s)	1350
XmnI	GAANNNNTTC	2 cut(s)	255, 1176
XspI	CTAG	6 cut(s)	30, 617, 804, 888, 1674, 1681
ZraI	GACGTC	1 cut(s)	235
ZrmI	AGTACT	1 cut(s)	59
Zsp2I	ATGCAT	1 cut(s)	924

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.