Basic Information

Type: gene

Biological Identity

Species

rosa_chinensis

Chromosome

Orthogroup ID

OG0010957

Physical Location & Seq

Genomic Coordinates Forward (+)

46405011 .. 46408096

Gene Structure 3086 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

PRQ25390

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1884 bp

ATGGACTCCGGAAAAGCCATGGCATCTACTCCTGCTACTACCCAATTGACCCCTTTCAAGTACTCCAATCGGGTCCTCTTGAAAACCATCTTGGAACGCAGTGATGGCGGCCTGGAGCTGATTGGTCAGAGAGTTCTCATAGGAGGGTGGGTCAAGTCCTCCAAGGAGGTCATGAAACAAACTCCTCCTCCGCCGCATCTACCGGCAGCCGATCATGAGACGGCTCGAGAACCCGAGGGCAAATCAGGCGTCAGCTGTGTCGAAATCCTTCAATCTCGGATACCATTTCTGAGAACCATTGTGAAGGTCTTGGGTGGAAGCAGCTCCTCCAGCAACACTCTTCGAGAAAGGCTCGATGCGGTGTTTAAGCCGGCGCCGCCCTCCACGGCATTCTTGAAAGTCAGTGATGGTTCTTGTCCTGCAACTCTTCAGGTAATTGTTGAATCGTCTATAGTCCCGCCTTGCCAGCTGTTGCATACCGGGACTTGCATCACGGTGGAAGGTGTGCTGCAGCAATCTTTGGTGCAAAGCAAACATGTCGTTGAGCTGAGAGTGGAGAAAGTGCTTCATATAGGAACAGTTGATTACAGCAAGTATCCATTGTCAAAGAAGAGAGTGCCAATTGAGAAGTTAAGGGAGTGCTTCCACATTCGCGCTCGGACAACCACGGTGGGATCTGTTATGCGAATCAGAAGTGCCCTGACTTTTGGGGCTCATACATTTTGTCACAACCATGGTTTTATTTCTGTGCAAGTACCCATTATAACTACAACAGACTGCAACGGGTTCAGTGAAAAGTACAAGGTCACTGGTCTTTTTGACAAGGCAGGTCAAACGGAGGAACCTAAAGCCATTGCTGAAATTGAAGGTGTTAGCCTTGAAGCTATTAAGGCTGCTGCAAAAGAGAAGAGTAGCTTAGTAGAGGAACTCAAGAGGACTGATAGCAATAAGGAAGCTCTGGCCGCTGCAGTACAGGATTTAAGGAAAACAAACGAATTGGCATCACAATTGGAAGCAAGAGAAAAATCAAAACTGAAAACTTCTCAAAAGCTTGACAATGTTAAGTCTTCCGAAGGTTCCTCCTCCTCCTCCCAAACTTTTCTAACCGTTTCTGGACGCCTGCATCTGGAGAGCTATGCATGTTCCCTTGGCAATGTTTACTCCTTCGGTCCCAGATTTCGAGCAGATAAAGGAGAGTCCCCTAAACATGTCCCAGAAATGTTGATGTTTGAAATTGAAATGGCTTTTTCACAACTAGAGGACGTAATGAATTGTGCAGATGACTTTTTCAAGTTCCTCTGCAAATGGGTGTTGGAAAATTGTCCGGAAGATATGAAATTTGTTACTGAAAGAATAGACAGGAACAGGATTGATCGTCTTCAATCAGTGATTTCAAGTTCATTTGAAAGAATTTCCTACGCTGAGGCATTAAATGTTTTAAACAAGGTTACGGATAAGAAAGTTGAAACAAAATTACAGTGGGGTGCTGCATTAACAGAAGAAGATTTAAGTCATTTGTCTGATGAGATTTACAAGAAGCCTGTAATTGTATACAATTATCCAAAAGAAGCTAAGCCGTTCTATGTACGGCTGAATGATGATGGAAAAACAGTTGCAGCATTTGATATGGTTGTACCAAAGATCGGAAAGTTAATTTCTGGTAGCCAAAATGAGGAGCGCACCCGTATACTAAGTACAAGGATCGAGGATTTGGGGTTGCCAAGAGAGCAGTATGAATGGTACTTGGATCTTCGCAGGCATGGAACTGTCAAGCACTCTGGCTTCAGTCTTGAGTTTGATCTTATGGTTCTCTTTGCCACTGGCCTTACTGATGTAAGAGATGCTATCCCCTTTCCCAGAAGTTATGGCAAAGCAAACTACTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004812 GO:0004816 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0006082 GO:0006139 GO:0006399 GO:0006412 GO:0006418 GO:0006421 GO:0006518 GO:0006520 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009507 GO:0009536 GO:0009987 GO:0010467 GO:0016070 GO:0016874 GO:0016875 GO:0019538 GO:0019752 GO:0034641 GO:0034645 GO:0034660 GO:0043038 GO:0043039 GO:0043043 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044281 GO:0044424 GO:0044444 GO:0044464 GO:0046483 GO:0071704 GO:0090304 GO:0140098 GO:0140101 GO:1901360 GO:1901564 GO:1901566 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00970 map00970

Pfam Domains

Protein Families

tRNA-synt_2

Protein Analysis

627

Amino Acids

69.85

Weight (kDa)

8.44

Isoelectric Point (pI)

48.01

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
tRNA-synt_2	PF00152	365 - 620	8.5e-39	tRNA synthetases class II (D, K and N)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0010957)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G07420 AT3G07420
fragaria_vesca	FvH4_2g17960
malus_domestica	MD05G1163500.v1.1 MD10G1153300.v1.1
prunus_persica	Prupe.8G192100_v2.0.a1
pyrus_communis	pycom05g15290 pycom10g13220
rosa_chinensis	RchiOBHm_Chr6g0283201
rosa_laevigata	RLG00000012837
rosa_multiflora	Rmu_sc0006459.1_g000001 Rmu_sc0013038.1_g000019
rosa_roxburghii	Rroxscaffold_2G00106680 Rroxscaffold_7G00185650
rosa_rugosa	Rorug06G0152600
rosa_samantha	Rh6AG265000 Rh6BG267900 Rh6CG267100 Rh6DG260300
rosa_wichuraiana	Rw6G022970

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	764
Acc36I	ACCTGC	1 cut(s)	818
AccB1I	GGYRCC	1 cut(s)	373
AccI	GTMKAC	2 cut(s)	1551, 1687
AccII	CGCG	1 cut(s)	654
AccIII	TCCGGA	2 cut(s)	8, 1324
AciI	CCGC	7 cut(s)	108, 191, 194, 359, 377, 458, 963
AclWI	GGATC	3 cut(s)	682, 1709, 1755
AcoI	YGGCCR	1 cut(s)	960
AcsI	RAATTY	2 cut(s)	1337, 1410
AcuI	CTGAAG	2 cut(s)	413, 1768
AcyI	GRCGYC	3 cut(s)	249, 374, 1117
AfaI	GTAC	8 cut(s)	62, 756, 800, 972, 1587, 1635, 1696, 1742
AfiI	CCNNNNNNNGG	3 cut(s)	1126, 1643, 1672
AflIII	ACRYGT	2 cut(s)	535, 1207
AjnI	CCWGG	1 cut(s)	111
AjuI	GAANNNNNNNTTGG	2 cut(s)	74, 106
Alw26I	GTCTC	1 cut(s)	212
AlwI	GGATC	3 cut(s)	682, 1709, 1755
Ama87I	CYCGRG	2 cut(s)	225, 233
Aor13HI	TCCGGA	2 cut(s)	8, 1324
AoxI	GGCC	3 cut(s)	109, 960, 1822
ApeKI	GCWGC	9 cut(s)	206, 321, 508, 511, 893, 896, 965, 1487, 1616
ApoI	RAATTY	2 cut(s)	1337, 1410
ArsI	GACNNNNNNTTYG	2 cut(s)	234, 266
Asp700I	GAANNNNTTC	1 cut(s)	267
AspLEI	GCGC	3 cut(s)	376, 656, 1680
AspS9I	GGNCC	2 cut(s)	73, 1169
AsuC2I	CCSGG	1 cut(s)	481
AvaI	CYCGRG	2 cut(s)	225, 233
AvaII	GGWCC	2 cut(s)	73, 1169
BaeGI	GKGCMC	1 cut(s)	700
BanI	GGYRCC	1 cut(s)	373
BanII	GRGCYC	1 cut(s)	715
BbsI	GAAGAC	2 cut(s)	1059, 1370
BbvCI	CCTCAGC	1 cut(s)	1422
BbvI	GCAGC	9 cut(s)	218, 333, 495, 523, 880, 883, 952, 1474, 1628
BccI	CCATC	4 cut(s)	95, 98, 401, 1595
BceAI	ACGGC	4 cut(s)	237, 402, 1561, 1604
BcgI	CGANNNNNNTGC	2 cut(s)	520, 554
BciT130I	CCWGG	1 cut(s)	113
BciVI	GTATCC	2 cut(s)	273, 606
BcnI	CCSGG	1 cut(s)	481
BcoDI	GTCTC	1 cut(s)	212
BfaI	CTAG	2 cut(s)	1256, 1882
BfmI	CTRYAG	3 cut(s)	450, 509, 966
BfoI	RGCGCY	1 cut(s)	377
BfuAI	ACCTGC	1 cut(s)	818
BfuI	GTATCC	2 cut(s)	273, 606
BlpI	GCTNAGC	1 cut(s)	1572
BmcAI	AGTACT	1 cut(s)	62
Bme1390I	CCNGG	2 cut(s)	113, 481
Bme18I	GGWCC	2 cut(s)	73, 1169
BmeT110I	CYCGRG	2 cut(s)	225, 233
BmgT120I	GGNCC	2 cut(s)	73, 1169
BmiI	GGNNCC	5 cut(s)	74, 375, 843, 1078, 1171
BmrFI	CCNGG	2 cut(s)	113, 481
BmsI	GCATC	7 cut(s)	32, 205, 346, 498, 1010, 1132, 1831
BpiI	GAAGAC	2 cut(s)	1059, 1370
BplI	GAGNNNNNCTC	2 cut(s)	336, 368
BpmI	CTGGAG	3 cut(s)	134, 313, 1148
Bpu10I	CCTNAGC	1 cut(s)	1422
Bpu1102I	GCTNAGC	1 cut(s)	1572
BpuEI	CTTGAG	2 cut(s)	914, 1811
BpuMI	CCSGG	1 cut(s)	481
BsaHI	GRCGYC	3 cut(s)	249, 374, 1117
BsaJI	CCNNGG	7 cut(s)	18, 162, 234, 384, 666, 733, 1147
BsaWI	WCCGGW	2 cut(s)	8, 1324
BsaXI	ACNNNNNCTCC	4 cut(s)	135, 165, 172, 202
Bsc4I	CCNNNNNNNGG	3 cut(s)	1126, 1643, 1672
Bse118I	RCCGGY	2 cut(s)	202, 370
Bse1I	ACTGG	2 cut(s)	814, 1825
Bse3DI	GCAATG	2 cut(s)	852, 1159
BseAI	TCCGGA	2 cut(s)	8, 1324
BseBI	CCWGG	1 cut(s)	113
BseDI	CCNNGG	7 cut(s)	18, 162, 234, 384, 666, 733, 1147
BseLI	CCNNNNNNNGG	3 cut(s)	1126, 1643, 1672
BseMI	GCAATG	2 cut(s)	852, 1159
BseMII	CTCAG	3 cut(s)	281, 539, 1413
BseNI	ACTGG	2 cut(s)	814, 1825
BseRI	GAGGAG	7 cut(s)	174, 177, 316, 1072, 1075, 1078, 1688
BseSI	GKGCMC	1 cut(s)	700
BseXI	GCAGC	9 cut(s)	218, 333, 495, 523, 880, 883, 952, 1474, 1628
BsgI	GTGCAG	1 cut(s)	1296
Bsh1236I	CGCG	1 cut(s)	654
BshFI	GGCC	3 cut(s)	111, 962, 1824
BshNI	GGYRCC	1 cut(s)	373
BsiHKCI	CYCGRG	2 cut(s)	225, 233
BsiSI	CCGG	5 cut(s)	9, 203, 371, 480, 1325
BslFI	GGGAC	5 cut(s)	440, 496, 1155, 1183, 1196
BslI	CCNNNNNNNGG	3 cut(s)	1126, 1643, 1672
BsmAI	GTCTC	1 cut(s)	212
BsmBI	CGTCTC	1 cut(s)	212
BsmFI	GGGAC	5 cut(s)	440, 496, 1155, 1183, 1196
BsmI	GAATGC	1 cut(s)	389
BsnI	GGCC	3 cut(s)	111, 962, 1824
BsoBI	CYCGRG	2 cut(s)	225, 233
Bsp1286I	GDGCHC	2 cut(s)	700, 715
Bsp13I	TCCGGA	2 cut(s)	8, 1324
Bsp143I	GATC	7 cut(s)	211, 674, 1372, 1641, 1701, 1747, 1798
Bsp1720I	GCTNAGC	1 cut(s)	1572
Bsp19I	CCATGG	2 cut(s)	18, 733
BspACI	CCGC	7 cut(s)	108, 191, 194, 359, 377, 458, 963
BspANI	GGCC	3 cut(s)	111, 962, 1824
BspCNI	CTCAG	3 cut(s)	282, 540, 1414
BspEI	TCCGGA	2 cut(s)	8, 1324
BspFNI	CGCG	1 cut(s)	654
BspHI	TCATGA	2 cut(s)	171, 214
BspLI	GGNNCC	5 cut(s)	74, 375, 843, 1078, 1171
BspMAI	CTGCAG	2 cut(s)	513, 970
BspMI	ACCTGC	1 cut(s)	818
BspPI	GGATC	3 cut(s)	682, 1709, 1755
BspT107I	GGYRCC	1 cut(s)	373
BsrDI	GCAATG	2 cut(s)	852, 1159
BsrFI	RCCGGY	2 cut(s)	202, 370
BsrI	ACTGG	2 cut(s)	814, 1825
BssAI	RCCGGY	2 cut(s)	202, 370
BssECI	CCNNGG	7 cut(s)	18, 162, 234, 384, 666, 733, 1147
BssMI	GATC	7 cut(s)	211, 674, 1372, 1641, 1701, 1747, 1798
BssNAI	GTATAC	2 cut(s)	1552, 1688
BssNI	GRCGYC	3 cut(s)	249, 374, 1117
BssT1I	CCWWGG	4 cut(s)	18, 162, 733, 1147
Bst1107I	GTATAC	2 cut(s)	1552, 1688
Bst2UI	CCWGG	1 cut(s)	113
Bst4CI	ACNGT	7 cut(s)	496, 580, 670, 1108, 1479, 1612, 1768
Bst6I	CTCTTC	4 cut(s)	345, 432, 605, 902
BstACI	GRCGYC	3 cut(s)	249, 374, 1117
BstC8I	GCNNGC	4 cut(s)	372, 467, 1121, 1757
BstDEI	CTNAG	6 cut(s)	290, 548, 916, 1422, 1572, 1691
BstDSI	CCRYGG	4 cut(s)	18, 384, 666, 733
BstFNI	CGCG	1 cut(s)	654
BstH2I	RGCGCY	1 cut(s)	377
BstHHI	GCGC	3 cut(s)	376, 656, 1680
BstKTI	GATC	7 cut(s)	214, 677, 1375, 1644, 1704, 1750, 1801
BstMAI	GTCTC	1 cut(s)	212
BstMBI	GATC	7 cut(s)	211, 674, 1372, 1641, 1701, 1747, 1798
BstMWI	GCNNNNNNNGC	8 cut(s)	105, 246, 330, 376, 466, 890, 962, 1726
BstNI	CCWGG	1 cut(s)	113
BstNSI	RCATGY	3 cut(s)	539, 1143, 1211
BstSCI	CCNGG	2 cut(s)	111, 479
BstSFI	CTRYAG	3 cut(s)	450, 509, 966
BstSLI	GKGCMC	1 cut(s)	700
BstUI	CGCG	1 cut(s)	654
BstV1I	GCAGC	9 cut(s)	218, 333, 495, 523, 880, 883, 952, 1474, 1628
BstV2I	GAAGAC	2 cut(s)	1059, 1370
BstX2I	RGATCY	2 cut(s)	674, 1747
BstYI	RGATCY	2 cut(s)	674, 1747
BstZ17I	GTATAC	2 cut(s)	1552, 1688
BsuI	GTATCC	2 cut(s)	273, 606
BsuRI	GGCC	3 cut(s)	111, 962, 1824
BtgI	CCRYGG	4 cut(s)	18, 384, 666, 733
BtsI	GCAGTG	1 cut(s)	106
BtsIMutI	CAGTG	7 cut(s)	106, 409, 796, 807, 1392, 1484, 1818
BveI	ACCTGC	1 cut(s)	818
Cac8I	GCNNGC	4 cut(s)	372, 467, 1121, 1757
CciI	TCATGA	2 cut(s)	171, 214
CfoI	GCGC	3 cut(s)	376, 656, 1680
Cfr10I	RCCGGY	2 cut(s)	202, 370
Cfr13I	GGNCC	2 cut(s)	73, 1169
CseI	GACGC	2 cut(s)	238, 1125
Csp6I	GTAC	8 cut(s)	61, 755, 799, 971, 1586, 1634, 1695, 1741
CspCI	CAANNNNNGTGG	2 cut(s)	651, 686
CviAII	CATG	8 cut(s)	19, 172, 215, 536, 734, 1140, 1208, 1760
CviQI	GTAC	8 cut(s)	61, 755, 799, 971, 1586, 1634, 1695, 1741
DdeI	CTNAG	6 cut(s)	290, 548, 916, 1422, 1572, 1691
DinI	GGCGCC	1 cut(s)	375
DpnI	GATC	7 cut(s)	213, 676, 1374, 1643, 1703, 1749, 1800
DpnII	GATC	7 cut(s)	211, 674, 1372, 1641, 1701, 1747, 1798
DraI	TTTAAA	1 cut(s)	1440
EaeI	YGGCCR	1 cut(s)	960
Eam1104I	CTCTTC	4 cut(s)	345, 432, 605, 902
EarI	CTCTTC	4 cut(s)	345, 432, 605, 902
EciI	GGCGGA	1 cut(s)	180
Eco130I	CCWWGG	4 cut(s)	18, 162, 733, 1147
Eco24I	GRGCYC	1 cut(s)	715
Eco47I	GGWCC	2 cut(s)	73, 1169
Eco57I	CTGAAG	2 cut(s)	413, 1768
Eco88I	CYCGRG	2 cut(s)	225, 233
EcoO109I	RGGNCCY	1 cut(s)	73
EcoRII	CCWGG	1 cut(s)	111
EcoT14I	CCWWGG	4 cut(s)	18, 162, 733, 1147
EcoT22I	ATGCAT	1 cut(s)	1141
EcoT38I	GRGCYC	1 cut(s)	715
EgeI	GGCGCC	1 cut(s)	375
EheI	GGCGCC	1 cut(s)	375
ErhI	CCWWGG	4 cut(s)	18, 162, 733, 1147
Esp3I	CGTCTC	1 cut(s)	212
FaeI	CATG	8 cut(s)	22, 175, 218, 539, 737, 1143, 1211, 1763
FalI	AAGNNNNNCTT	2 cut(s)	899, 931
FaqI	GGGAC	5 cut(s)	440, 496, 1155, 1183, 1196
FatI	CATG	8 cut(s)	18, 171, 214, 535, 733, 1139, 1207, 1759
FauI	CCCGC	1 cut(s)	465
FblI	GTMKAC	2 cut(s)	1551, 1687
FriOI	GRGCYC	1 cut(s)	715
FspBI	CTAG	2 cut(s)	1256, 1882
GlaI	GCGC	3 cut(s)	375, 655, 1679
GsuI	CTGGAG	3 cut(s)	134, 313, 1148
HaeII	RGCGCY	1 cut(s)	377
HaeIII	GGCC	3 cut(s)	111, 962, 1824
HapII	CCGG	5 cut(s)	9, 203, 371, 480, 1325
HgaI	GACGC	2 cut(s)	238, 1125
HhaI	GCGC	3 cut(s)	376, 656, 1680
Hin1I	GRCGYC	3 cut(s)	249, 374, 1117
Hin1II	CATG	8 cut(s)	22, 175, 218, 539, 737, 1143, 1211, 1763
Hin6I	GCGC	3 cut(s)	374, 654, 1678
HinP1I	GCGC	3 cut(s)	374, 654, 1678
HindIII	AAGCTT	1 cut(s)	1049
HinfI	GANTC	4 cut(s)	5, 443, 687, 1196
HpaII	CCGG	5 cut(s)	9, 203, 371, 480, 1325
Hpy166II	GTNNAC	3 cut(s)	1159, 1552, 1688
Hpy188I	TCNGA	8 cut(s)	129, 279, 291, 660, 692, 1072, 1522, 1646
Hpy8I	GTNNAC	3 cut(s)	1159, 1552, 1688
HpyAV	CCTTC	6 cut(s)	278, 298, 494, 860, 1067, 1174
HpyCH4III	ACNGT	7 cut(s)	496, 580, 670, 1108, 1479, 1612, 1768
HpyCH4IV	ACGT	1 cut(s)	1263
HpyF10VI	GCNNNNNNNGC	8 cut(s)	105, 246, 330, 376, 466, 890, 962, 1726
HpyF3I	CTNAG	6 cut(s)	290, 548, 916, 1422, 1572, 1691
HpySE526I	ACGT	1 cut(s)	1263
Hsp92I	GRCGYC	3 cut(s)	249, 374, 1117
Hsp92II	CATG	8 cut(s)	22, 175, 218, 539, 737, 1143, 1211, 1763
HspAI	GCGC	3 cut(s)	374, 654, 1678
KasI	GGCGCC	1 cut(s)	373
Kpn2I	TCCGGA	2 cut(s)	8, 1324
KroI	GCCGGC	1 cut(s)	370
KroNI	GCCGGC	1 cut(s)	372
Kzo9I	GATC	7 cut(s)	211, 674, 1372, 1641, 1701, 1747, 1798
LmnI	GCTCC	3 cut(s)	115, 329, 1675
Lsp1109I	GCAGC	9 cut(s)	218, 333, 495, 523, 880, 883, 952, 1474, 1628
LweI	GCATC	7 cut(s)	32, 205, 346, 498, 1010, 1132, 1831
MaeI	CTAG	2 cut(s)	1256, 1882
MaeII	ACGT	1 cut(s)	1263
MaeIII	GTNAC	4 cut(s)	725, 805, 1342, 1447
MalI	GATC	7 cut(s)	213, 676, 1374, 1643, 1703, 1749, 1800
MboI	GATC	7 cut(s)	211, 674, 1372, 1641, 1701, 1747, 1798
MfeI	CAATTG	3 cut(s)	44, 621, 1007
MflI	RGATCY	2 cut(s)	674, 1747
MhlI	GDGCHC	2 cut(s)	700, 715
Mly113I	GGCGCC	1 cut(s)	374
MlyI	GAGTC	1 cut(s)	1205
MmeI	TCCRAC	1 cut(s)	1293
Mph1103I	ATGCAT	1 cut(s)	1141
MroI	TCCGGA	2 cut(s)	8, 1324
MroNI	GCCGGC	1 cut(s)	370
MroXI	GAANNNNTTC	1 cut(s)	267
MslI	CAYNNNNRTG	2 cut(s)	494, 732
MspA1I	CMGCKG	3 cut(s)	255, 469, 965
MspI	CCGG	5 cut(s)	9, 203, 371, 480, 1325
MspR9I	CCNGG	2 cut(s)	113, 481
MunI	CAATTG	3 cut(s)	44, 621, 1007
Mva1269I	GAATGC	1 cut(s)	389
MvaI	CCWGG	1 cut(s)	113
MvnI	CGCG	1 cut(s)	654
MwoI	GCNNNNNNNGC	8 cut(s)	105, 246, 330, 376, 466, 890, 962, 1726
NaeI	GCCGGC	1 cut(s)	372
NarI	GGCGCC	1 cut(s)	374
NciI	CCSGG	1 cut(s)	481
NcoI	CCATGG	2 cut(s)	18, 733
NdeII	GATC	7 cut(s)	211, 674, 1372, 1641, 1701, 1747, 1798
NgoMIV	GCCGGC	1 cut(s)	370
NlaIII	CATG	8 cut(s)	22, 175, 218, 539, 737, 1143, 1211, 1763
NlaIV	GGNNCC	5 cut(s)	74, 375, 843, 1078, 1171
NmuCI	GTSAC	2 cut(s)	725, 805
NsiI	ATGCAT	1 cut(s)	1141
NspI	RCATGY	3 cut(s)	539, 1143, 1211
PaeR7I	CTCGAG	1 cut(s)	225
PagI	TCATGA	2 cut(s)	171, 214
PciI	ACATGT	2 cut(s)	535, 1207
PctI	GAATGC	1 cut(s)	389
PdiI	GCCGGC	1 cut(s)	372
PdmI	GAANNNNTTC	1 cut(s)	267
PfeI	GAWTC	2 cut(s)	443, 687
PleI	GAGTC	1 cut(s)	1204
PluTI	GGCGCC	1 cut(s)	377
PpsI	GAGTC	1 cut(s)	1204
PpuMI	RGGWCCY	1 cut(s)	73
PscI	ACATGT	2 cut(s)	535, 1207
PsiI	TTATAA	1 cut(s)	764
Psp5II	RGGWCCY	1 cut(s)	73
Psp6I	CCWGG	1 cut(s)	111
PspGI	CCWGG	1 cut(s)	111
PspN4I	GGNNCC	5 cut(s)	74, 375, 843, 1078, 1171
PspPI	GGNCC	2 cut(s)	73, 1169
PspPPI	RGGWCCY	1 cut(s)	73
PstI	CTGCAG	2 cut(s)	513, 970
PsuI	RGATCY	2 cut(s)	674, 1747
PvuII	CAGCTG	2 cut(s)	255, 469
RsaI	GTAC	8 cut(s)	62, 756, 800, 972, 1587, 1635, 1696, 1742
RsaNI	GTAC	8 cut(s)	61, 755, 799, 971, 1586, 1634, 1695, 1741
RseI	CAYNNNNRTG	2 cut(s)	494, 732
Sau3AI	GATC	7 cut(s)	211, 674, 1372, 1641, 1701, 1747, 1798
Sau96I	GGNCC	2 cut(s)	73, 1169
ScaI	AGTACT	1 cut(s)	62
SchI	GAGTC	1 cut(s)	1205
ScrFI	CCNGG	2 cut(s)	113, 481
SduI	GDGCHC	2 cut(s)	700, 715
SfaNI	GCATC	7 cut(s)	32, 205, 346, 498, 1010, 1132, 1831
SfcI	CTRYAG	3 cut(s)	450, 509, 966
SfoI	GGCGCC	1 cut(s)	375
Sfr274I	CTCGAG	1 cut(s)	225
SinI	GGWCC	2 cut(s)	73, 1169
SlaI	CTCGAG	1 cut(s)	225
SmiMI	CAYNNNNRTG	2 cut(s)	494, 732
SmlI	CTYRAG	3 cut(s)	225, 929, 1790
SmoI	CTYRAG	3 cut(s)	225, 929, 1790
SsiI	CCGC	7 cut(s)	108, 191, 194, 359, 377, 458, 963
SspDI	GGCGCC	1 cut(s)	373
SspMI	CTAG	2 cut(s)	1256, 1882
StyD4I	CCNGG	2 cut(s)	111, 479
StyI	CCWWGG	4 cut(s)	18, 162, 733, 1147
TaaI	ACNGT	7 cut(s)	496, 580, 670, 1108, 1479, 1612, 1768
TaiI	ACGT	1 cut(s)	1266
TaqI	TCGA	6 cut(s)	226, 261, 343, 354, 1180, 1704
TaqII	GACCGA	1 cut(s)	1157
TatI	WGTACW	4 cut(s)	60, 798, 970, 1694
TauI	GCSGC	4 cut(s)	111, 196, 379, 965
TfiI	GAWTC	2 cut(s)	443, 687
TscAI	CASTG	7 cut(s)	106, 409, 796, 814, 1392, 1484, 1825
TseFI	GTSAC	2 cut(s)	725, 805
TseI	GCWGC	9 cut(s)	206, 321, 508, 511, 893, 896, 965, 1487, 1616
Tsp45I	GTSAC	2 cut(s)	725, 805
TspDTI	ATGAA	6 cut(s)	188, 557, 1283, 1349, 1389, 1749
TspGWI	ACGGA	2 cut(s)	851, 1466
TspRI	CASTG	7 cut(s)	106, 409, 796, 814, 1392, 1484, 1825
VpaK11BI	GGWCC	2 cut(s)	73, 1169
XapI	RAATTY	2 cut(s)	1337, 1410
XceI	RCATGY	3 cut(s)	539, 1143, 1211
XhoI	CTCGAG	1 cut(s)	225
XmiI	GTMKAC	2 cut(s)	1551, 1687
XmnI	GAANNNNTTC	1 cut(s)	267
XspI	CTAG	2 cut(s)	1256, 1882
ZrmI	AGTACT	1 cut(s)	62
Zsp2I	ATGCAT	1 cut(s)	1141

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.