Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr05

Orthogroup ID

OG0003289

Physical Location & Seq

Genomic Coordinates Reverse (-)

31336025 .. 31347813

Gene Structure 11789 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD05G1184900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 4554 bp

ATGGCTGCTGCTATTAGTCTTGTGGTTGGATCTCTTGTTTGGATCGAGGATCCTGAAGAAGCTTGGCTCAATGGTGAAATTGTGGAGATTAAAGGTGAAAACTACAAGGTTCTCTGCACTTCAGGAAAGACGGTTGTCGTTAAAGCCTCAAATGTCTATCCTAAAGATGCTGAGGCCCCACCCTGTGGAGTGGATGATATGACCAAGCTGGCTTATTTGCATGAACCAGGTGTCCTAGATAATTTACGGTCAAGATATGATATCAATGAAATATATACGTACACAGGGAGCATTTTGATTGCTGTGAACCCTTTTTGTAGACTACCTCATCTGTATGATAGCCATATGATGGAACAATATAAAGGGGCTGATTTCGGTGAACTAAGCCCACACCCGTTTGCTGTTGCAGACGCAGCATATAGACTTATGATTAATGACGGAATAAGTCAGTCAATATTGGTTAGTGGTGAGAGTGGGGCTGGTAAAACAGAAAGCACCAAACTGCTTATGCGCTATCTTGCCTACATGGGAGGGAAAGCTGCTGCTGAAGGGAGGAGTGTTGAGCAGCAAGTCTTGGAGTCAAATCCTGTTTTAGAAGCATTTGGGAATGCGAAGACTGTTAGAAACAATAATTCCAGTCGTTTTGGTAAGTTTGTGGAGCTTCAGTTTGATAAGAATGGGAGGATCTCCGGGGCTGCAATAAGAACATACTTGCTTGAAAGATCGCGTGTTTGTCAGGTGTCCAGTCCTGAAAGAAACTATCACTGTTTCTACATGCTTTGCGCTGCACCACCTGAGGATGTTCAAAGGTTCAAATTGGGACACCCAAAAACATTTCACTATTTGAATCAATCAGATTGTATTGAATTGGATGGGCTTGATGATGCCGAAGAATACATTGCTACAAGGAAGGCAATGGAGGTTGTTGGAATAAGTACTGAAGAACAGGATGCCATATTTCGAGTTGTGGCAGCAATTCTACATCTCGGAAACATTGAATTTGCAAAGGGAAAGGAAATGGACTCGTCCATGCCGAAGGACAACAAGTCTTTGTTCCACCTAAAAACTGCTGCTGAGCTTTTCATGTGTGATGCGAAGGCGCTAGAAGATTCTCTTTGCAAACGTGTTATTGTTACGCGTGATGAAACAATCACAAAATGGCTGGATCCAGAAGCCGCAGCTGTCAGTAGAGATGCTTTGGCTAAAGTTGTGTACTCGAGATTGTTTGATTGGCTGGTGGATAAGATTAACAATTCAATTGGTCAGGACCCTGACTCAAAGTTCTTAATTGGAGTTCTCGACATTTATGGATTTGAGAGTTTCAAAACTAACAGTTTTGAGCAATTTTGCATCAATTTGACGAATGAGAAGCTTCAGCAACATTTCAATCAGCACGTTTTCAAAATGGAGCAAGAGGAATATACAAAAGAAGAGATTGATTGGAGTTACATTGAATTTGTTGACAACCAAGATATCCTAGATATGATTGAAAAGAAACCTGGTGGCATAATTGCTCTTCTCGATGAAGCTTGTATGTTTCCAAGATCGACACATGAAACATTTGCTCAGAAGTTATATCAAACATTCAAAAATCATAAGCGCTTCACCAAACCAAAATTGTCACAAAGTGACTTCACAATTTGCCATTATGCTGGTGATGTCACTTATCAAACTGAATTGTTTCTGGACAAGAACAAGGACTATGTGGTTGCTGAGCATCAAGCGCTTTTGAGTGCTTCTACATGTTCCTTTGTCTCGGGCATGTTTACGTCGTTAGTTGAGGATTCATCCAAGTCGTCAAAGTTCTCTTCAATAGGTTCTCGGTTTAAGCAACAACTGCAACAATTGCTTGAAACTCTCAGTTCTACGGAGCCACATTATATTCGGTGTGTAAAACCCAACAATGTTCTTAAGCCAGCAATCTTTGAGAACAAAAATGTTCTGCAACAACTTCGTTGTGGGGGGGTCATGGAAGCAATCAGGATAAGCTGTGCTGGATATCCTACTAGAAAAGCTTTTGTTGAATTTGTAGACCGATTTGGCCTCCTTGCACCCGAAGTTCTGGATGGGAGCACTGATGAGGTCAATGCTTGCAAGAAACTTTTAGAGAAGGTGGGCCTAGAAGGCTATCAGATTGGTAAAACGAAGGTCTTCCTTCGGGCTGGTCAGATGGCGGAATTGGATGCTCGTAGAAGTGAGGTCTTGGGAAGATCAGCTAGTATTATTCAAAGGAAAGTTCGTTCTTATTTGGCTAAAAGAAGTTTTATTTTGCTCCGTCTTTCTGCAATACGACTCCAAGCTGCTTGTAGAGGACATCTTGCTCGACATGTCTATCAGGGCATGAGGAGAGAAGCTTCCAGTCAGATGATCCAAAGGCATTTGCGCATGTATCTTGCTAGGAAAGCTTACAAAGAATTGTATTGCTCTGCTGTATCTATTCAGACCGGTATGCGAGGGTTAACTGCACGTAATGAGCTACGCTTTAGAAGGCAGACCAGAGCAGCAATTATCATCCAGAGTCACACTCGCAAGTTCTTGGCACGGTTGCATTATAGGAAGACAAAGAAAGCTGCAATTACCACACAATGTGCTTGGAGAGGAAAGGTTGCTTGTATGGAACTACGAAAGCTTAAGATGGCTGCAAGGGAAACGGGAGCTCTCCAAGCTGCCAAAAATAAATTAGAAAAGCAAGTTGAAGAATTGACATGGAGATTACAGCTGGAGAAACGCATAAGGGCCGACTTGGAAGAAGCCAAAACACAAGAAAATGAAAAACTACAGTCTGCTTTGCAAGAGATGCAAGTTCAATTTAAGGAAACGAAGGCGATGCTTGAGAAGGAGCGTGAAGCCTTAAAAAGAGCAGAAAAAGTAGTCTCGGTAATACAGGAGGTTCCAGTTGTTGACCATGGCTTGATGGAGAAGCTAACCAATGAAAATGAACAACTTAAGGCTTTAGTGAATTCACTGGAGATAAAAATTGACGAAACAGAAAAGAAATATGAAGAGGCAAACAAAATGAGTGAAGAAAGGTTGAAGCAAGCTTTGGAGGCAGAATCACAGATTATTAAGTTAAAGACGAACATACAAAGGTTATTTGAGCTTGAAGAGAAATTTGCTGACATTGAGTCTGAAAACAAAATTCTTCGGCAACAGCAAATAAGTACACCTGTTAAAAGGGCACATGAGCACCCACCAATTCCACCAACTCCAGAAACTCATGTAGCACTCCTCTCTTATAGAGTAGAAAATGGTCACCATTTGAGCAAAGAGAGCAGAGCCAATGAACCACAAAGTGCAACACCGAAGAAGTTTGGCACTGAATCTGATAGCAAGTTGAGGAGATCACTTGTTGAACGTCAACATGAGAGTGTTGATGCTCTTATCAATTGTGTCGTGAAAAACGTTGGGTTCAGTCAAGGAAAGCCTGTTGCGGCCATTACCATCTACAAGTGTCTTCTCCACTGGAAATCTTTTGAGGCTGAAAGGACTAGTGTGTTTGATCGCCTCATCCAGATGATTGGTTCCGAAATTGAGAACCAAGACAACAATGATCACATGGCATACTGGCTATCAAATACATCTGCATTATTGTTCTTACTCCAACGAAGTCTGAAGGGTGCTGGTGCAAAGAAACCACCCGCTCCAACATCTCTCTTTGGGAGGATGACCATGGGTTTTCGCTCATCCCCTTCTTCTGCAAACCTTGCACCTGCACTTGATGTAGTACGCCAAGTGGAAGCCAAGTACCCAGCATTATTATTCAAGCAGCAGCTTACAGCATATGTGGAGAAAATATATGGCATTATTCGAGACAACTTGAAAAGAGAGTTGAATCCATTGCTTTCTTTGTGTATCCAGGCACCAAGAGCGTCAAAGGGTGTACTAAGATCTGGTCGGTCCTTTGGGAAAGATTCTCCAGCGAGTCACTGGCTGAGCATTATTGACAGCCTTAGCACTTTCCTTAGCACATTGAAAGAAAATTTTGTGCCTCCTGTTCTTGTCAAGGAGATCTTTACTCAGACTTTCTCCTATATTAATGTTCAACTGTTTAATAGTCTTCTTCTACGTCGTGAATGTTGTACATTCAGCAATGGGGAGTATGTGAAATCTGGATTGGCTGAATTGGAGCTGTGGTGTTGTCAAGCAAAAGAAGAGTATGCGGGCTCATCGTGGGATGAACTTAAGCACATAAGACAAGCAGTTGGTTTCCTGGTCATACATCAGAAGTACAGAATTTCATATGATGAAATCACCAATGATCTGTGCCCTATCCTGAGTGTTCAACAGCTGTACAGAATATGTACTCTCTATTGGGATGATAACTACAACACCCGAAGTGTATCTCCAGATGTAATTTCCAGTATGAGAGTACTTATGACTGAGGACTCGAACAATGCTGTCAGCAACTCATTTTTGTTGGATGACAATTCCAGCATCCCCTTCTCGGTTGATGACCTCTCTACGTCCCTGCAAGAGAAGGAGTTTTCAGATGTCCAACCAGCAGAGGAACTTCTTGAGCATCCTGCCTTTGAGTTTTTACATGAGCGAGGCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000146 GO:0000166 GO:0000331 GO:0003674 GO:0003774 GO:0003779 GO:0003824 GO:0005488 GO:0005515 GO:0005524 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005856 GO:0006928 GO:0008092 GO:0008144 GO:0008150 GO:0009987 GO:0015629 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017076 GO:0017111 GO:0030029 GO:0030048 GO:0030554 GO:0030898 GO:0031410 GO:0031982 GO:0032553 GO:0032555 GO:0032559 GO:0033275 GO:0035639 GO:0036094 GO:0042623 GO:0042641 GO:0043167 GO:0043168 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043531 GO:0044422 GO:0044424 GO:0044430 GO:0044444 GO:0044446 GO:0044464 GO:0044877 GO:0051015 GO:0051179 GO:0051234 GO:0051640 GO:0051641 GO:0051656 GO:0070252 GO:0097159 GO:0097367 GO:0097708 GO:0140027 GO:1901265 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DIL IQ Myosin_N Myosin_head

Protein Analysis

1518

Amino Acids

172.23

Weight (kDa)

7.65

Isoelectric Point (pI)

48.26

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Myosin_N	PF02736	9 - 52	1.5e-14	Myosin N-terminal SH3-like domain
Myosin_head	PF00063	64 - 720	5.3e-256	Myosin head (motor domain)
DIL	PF01843	1337 - 1441	1.3e-29	DIL domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003289)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G04160 AT1G04160 AT2G20290 AT2G20290 AT2G20290 AT2G20290 AT4G28710 AT5G43900 AT5G43900 AT5G43900
fragaria_vesca	FvH4_2g20160 FvH4_2g20160 FvH4_2g20160 FvH4_2g20160 FvH4_2g20160 FvH4_2g20160 FvH4_2g20160 FvH4_2g20160
malus_domestica	MD05G1184900.v1.1 MD10G1172900.v1.1
prunus_persica	Prupe.8G211100_v2.0.a1 Prupe.8G211100_v2.0.a1
pyrus_communis	pycom05g16970 pycom10g14900
rosa_chinensis	RchiOBHm_Chr6g0285891
rosa_laevigata	RLG00000012601
rosa_multiflora	Rmu_sc0004808.1_g000001
rosa_roxburghii	Rroxscaffold_7G00182630 Rroxscaffold_7G00183900 Rroxscaffold_7G00184180
rosa_rugosa	Rorug06G0177400
rosa_samantha	Rh6AG289400 Rh6BG291200 Rh6CG292200 Rh6DG284300
rosa_wichuraiana	Rw6G024930

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	3718
Acc16I	TGCGCA	1 cut(s)	2384
Acc36I	ACCTGC	1 cut(s)	3718
AccB1I	GGYRCC	1 cut(s)	3859
AccB7I	CCANNNNNTGG	2 cut(s)	185, 349
AccBSI	CCGCTC	1 cut(s)	3641
AccI	GTMKAC	2 cut(s)	319, 2031
AccII	CGCG	2 cut(s)	727, 1138
AciI	CCGC	5 cut(s)	1176, 2174, 3431, 3639, 4160
AclI	AACGTT	1 cut(s)	3402
AclWI	GGATC	8 cut(s)	37, 44, 50, 57, 692, 1160, 1173, 2362
AcoI	YGGCCR	1 cut(s)	3432
AcsI	RAATTY	8 cut(s)	998, 1454, 2024, 2959, 3110, 3138, 3979, 4233
AcuI	CTGAAG	7 cut(s)	75, 105, 567, 647, 960, 1358, 3632
AdeI	CACNNNGTG	4 cut(s)	185, 1628, 2588, 3293
AfeI	AGCGCT	2 cut(s)	1601, 1725
AfiI	CCNNNNNNNGG	3 cut(s)	185, 349, 4444
AflII	CTTAAG	4 cut(s)	1910, 2630, 2945, 4181
AflIII	ACRYGT	4 cut(s)	1123, 1136, 1742, 2326
AgeI	ACCGGT	1 cut(s)	2444
AhdI	GACNNNNNGTC	2 cut(s)	1045, 3124
AhlI	ACTAGT	1 cut(s)	3488
AjnI	CCWGG	4 cut(s)	226, 1498, 3855, 4209
AjuI	GAANNNNNNNTTGG	4 cut(s)	3026, 3058, 4097, 4129
AloI	GAACNNNNNNTCC	4 cut(s)	216, 248, 2224, 2256
Alw21I	GWGCWC	3 cut(s)	2075, 2659, 3189
Alw26I	GTCTC	3 cut(s)	1759, 2878, 3804
AlwI	GGATC	8 cut(s)	37, 44, 50, 57, 692, 1160, 1173, 2362
Ama87I	CYCGRG	2 cut(s)	1216, 1756
Aor51HI	AGCGCT	2 cut(s)	1601, 1725
AoxI	GGCC	5 cut(s)	174, 2041, 2116, 2736, 3432
ApoI	RAATTY	8 cut(s)	998, 1454, 2024, 2959, 3110, 3138, 3979, 4233
AseI	ATTAAT	2 cut(s)	432, 4035
AsiGI	ACCGGT	1 cut(s)	2444
Asp700I	GAANNNNTTC	2 cut(s)	1680, 2150
AspLEI	GCGC	6 cut(s)	513, 785, 1102, 1602, 1726, 2385
AspS9I	GGNCC	5 cut(s)	175, 1267, 2116, 2736, 3897
AsuC2I	CCSGG	1 cut(s)	691
AsuHPI	GGTGA	8 cut(s)	86, 107, 389, 479, 1597, 1667, 3245, 4243
AvaI	CYCGRG	2 cut(s)	1216, 1756
AvaII	GGWCC	2 cut(s)	1267, 3897
AxyI	CCTNAGG	1 cut(s)	795
BaeGI	GKGCMC	2 cut(s)	3181, 4268
BamHI	GGATCC	2 cut(s)	49, 1165
BanI	GGYRCC	1 cut(s)	3859
BanII	GRGCYC	2 cut(s)	2659, 4166
BbsI	GAAGAC	5 cut(s)	620, 2143, 2564, 3446, 4049
Bbv12I	GWGCWC	3 cut(s)	2075, 2659, 3189
BbvCI	CCTCAGC	1 cut(s)	171
BccI	CCATC	7 cut(s)	343, 866, 2060, 2164, 2629, 2908, 3449
BcgI	CGANNNNNNTGC	4 cut(s)	1934, 1968, 2809, 2843
BciT130I	CCWGG	4 cut(s)	228, 1500, 3857, 4211
BciVI	GTATCC	1 cut(s)	3863
BclI	TGATCA	1 cut(s)	3550
BcnI	CCSGG	1 cut(s)	691
BcoDI	GTCTC	3 cut(s)	1759, 2878, 3804
BcuI	ACTAGT	1 cut(s)	3488
BfaI	CTAG	8 cut(s)	236, 1103, 1478, 2007, 2120, 2217, 2397, 3489
BfmI	CTRYAG	1 cut(s)	2777
BfoI	RGCGCY	3 cut(s)	1103, 1603, 1727
BfrI	CTTAAG	4 cut(s)	1910, 2630, 2945, 4181
BfuAI	ACCTGC	1 cut(s)	3718
BfuI	GTATCC	1 cut(s)	3863
BglI	GCCNNNNNGGC	1 cut(s)	2124
BglII	AGATCT	2 cut(s)	3887, 4008
BlpI	GCTNAGC	3 cut(s)	1074, 1713, 3932
BmcAI	AGTACT	2 cut(s)	937, 4371
Bme1390I	CCNGG	5 cut(s)	228, 691, 1500, 3857, 4211
Bme18I	GGWCC	2 cut(s)	1267, 3897
BmeRI	GACNNNNNGTC	2 cut(s)	1045, 3124
BmeT110I	CYCGRG	2 cut(s)	1216, 1756
BmgT120I	GGNCC	5 cut(s)	175, 1267, 2116, 2736, 3897
BmiI	GGNNCC	8 cut(s)	51, 177, 1167, 1269, 1872, 2892, 3523, 3861
BmrFI	CCNGG	5 cut(s)	228, 691, 1500, 3857, 4211
BoxI	GACNNNNGTC	1 cut(s)	134
BpiI	GAAGAC	5 cut(s)	620, 2143, 2564, 3446, 4049
BplI	GAGNNNNNCTC	2 cut(s)	2509, 2541
BpmI	CTGGAG	5 cut(s)	2741, 2987, 3192, 3900, 4329
Bpu10I	CCTNAGC	3 cut(s)	171, 3950, 3962
Bpu1102I	GCTNAGC	3 cut(s)	1074, 1713, 3932
BpuEI	CTTGAG	2 cut(s)	2852, 4535
BpuMI	CCSGG	1 cut(s)	691
BsaAI	YACGTR	2 cut(s)	279, 2468
BsaJI	CCNNGG	3 cut(s)	690, 2905, 3669
BsaWI	WCCGGW	1 cut(s)	2444
BsaXI	ACNNNNNCTCC	4 cut(s)	650, 680, 4118, 4148
Bsc4I	CCNNNNNNNGG	3 cut(s)	185, 349, 4444
Bse118I	RCCGGY	1 cut(s)	2444
Bse1I	ACTGG	9 cut(s)	636, 744, 2358, 2894, 2970, 3467, 3569, 3932, 4359
Bse21I	CCTNAGG	1 cut(s)	795
Bse3DI	GCAATG	4 cut(s)	897, 921, 3836, 4096
BseBI	CCWGG	4 cut(s)	228, 1500, 3857, 4211
BseDI	CCNNGG	3 cut(s)	690, 2905, 3669
BseLI	CCNNNNNNNGG	3 cut(s)	185, 349, 4444
BseMI	GCAATG	4 cut(s)	897, 921, 3836, 4096
BseNI	ACTGG	9 cut(s)	636, 744, 2358, 2894, 2970, 3467, 3569, 3932, 4359
BseRI	GAGGAG	4 cut(s)	568, 2359, 3218, 3352
BseSI	GKGCMC	2 cut(s)	3181, 4268
BseYI	CCCAGC	1 cut(s)	3748
BsgI	GTGCAG	4 cut(s)	100, 771, 2448, 3696
Bsh1236I	CGCG	2 cut(s)	727, 1138
BshFI	GGCC	5 cut(s)	176, 2043, 2118, 2738, 3434
BshNI	GGYRCC	1 cut(s)	3859
BshTI	ACCGGT	1 cut(s)	2444
BsiHKAI	GWGCWC	3 cut(s)	2075, 2659, 3189
BsiHKCI	CYCGRG	2 cut(s)	1216, 1756
BsiSI	CCGG	2 cut(s)	690, 2445
BslFI	GGGAC	2 cut(s)	834, 4450
BslI	CCNNNNNNNGG	3 cut(s)	185, 349, 4444
BsmAI	GTCTC	3 cut(s)	1759, 2878, 3804
BsmFI	GGGAC	2 cut(s)	834, 4450
BsmI	GAATGC	1 cut(s)	613
BsnI	GGCC	5 cut(s)	176, 2043, 2118, 2738, 3434
BsoBI	CYCGRG	2 cut(s)	1216, 1756
Bsp1286I	GDGCHC	6 cut(s)	2075, 2659, 3181, 3189, 4166, 4268
Bsp1407I	TGTACA	2 cut(s)	4079, 4290
Bsp1720I	GCTNAGC	3 cut(s)	1074, 1713, 3932
Bsp19I	CCATGG	2 cut(s)	2905, 3669
BspACI	CCGC	5 cut(s)	1176, 2174, 3431, 3639, 4160
BspANI	GGCC	5 cut(s)	176, 2043, 2118, 2738, 3434
BspFNI	CGCG	2 cut(s)	727, 1138
BspLI	GGNNCC	8 cut(s)	51, 177, 1167, 1269, 1872, 2892, 3523, 3861
BspMI	ACCTGC	1 cut(s)	3718
BspPI	GGATC	8 cut(s)	37, 44, 50, 57, 692, 1160, 1173, 2362
BspQI	GCTCTTC	1 cut(s)	1521
BspT107I	GGYRCC	1 cut(s)	3859
BspTI	CTTAAG	4 cut(s)	1910, 2630, 2945, 4181
BsrBI	CCGCTC	1 cut(s)	3641
BsrDI	GCAATG	4 cut(s)	897, 921, 3836, 4096
BsrFI	RCCGGY	1 cut(s)	2444
BsrGI	TGTACA	2 cut(s)	4079, 4290
BsrI	ACTGG	9 cut(s)	636, 744, 2358, 2894, 2970, 3467, 3569, 3932, 4359
BssAI	RCCGGY	1 cut(s)	2444
BssECI	CCNNGG	3 cut(s)	690, 2905, 3669
BssT1I	CCWWGG	2 cut(s)	2905, 3669
Bst2UI	CCWGG	4 cut(s)	228, 1500, 3857, 4211
Bst4CI	ACNGT	8 cut(s)	133, 249, 619, 767, 1334, 2544, 2781, 4047
Bst6I	CTCTTC	6 cut(s)	1425, 1521, 1813, 2997, 3099, 4146
BstAFI	CTTAAG	4 cut(s)	1910, 2630, 2945, 4181
BstAPI	GCANNNNNTGC	4 cut(s)	1837, 1846, 2797, 3704
BstAUI	TGTACA	2 cut(s)	4079, 4290
BstBAI	YACGTR	2 cut(s)	279, 2468
BstC8I	GCNNGC	5 cut(s)	210, 1917, 2092, 3039, 4162
BstDSI	CCRYGG	2 cut(s)	2905, 3669
BstEII	GGTNACC	1 cut(s)	3251
BstFNI	CGCG	2 cut(s)	727, 1138
BstH2I	RGCGCY	3 cut(s)	1103, 1603, 1727
BstHHI	GCGC	6 cut(s)	513, 785, 1102, 1602, 1726, 2385
BstMAI	GTCTC	3 cut(s)	1759, 2878, 3804
BstNI	CCWGG	4 cut(s)	228, 1500, 3857, 4211
BstNSI	RCATGY	5 cut(s)	778, 1746, 1765, 2330, 2389
BstPAI	GACNNNNGTC	1 cut(s)	134
BstPI	GGTNACC	1 cut(s)	3251
BstSCI	CCNGG	5 cut(s)	226, 689, 1498, 3855, 4209
BstSFI	CTRYAG	1 cut(s)	2777
BstSLI	GKGCMC	2 cut(s)	3181, 4268
BstSNI	TACGTA	1 cut(s)	279
BstUI	CGCG	2 cut(s)	727, 1138
BstV2I	GAAGAC	5 cut(s)	620, 2143, 2564, 3446, 4049
BstX2I	RGATCY	6 cut(s)	29, 49, 684, 1165, 3887, 4008
BstXI	CCANNNNNNTGG	2 cut(s)	1652, 3518
BstYI	RGATCY	6 cut(s)	29, 49, 684, 1165, 3887, 4008
Bsu36I	CCTNAGG	1 cut(s)	795
BsuI	GTATCC	1 cut(s)	3863
BsuRI	GGCC	5 cut(s)	176, 2043, 2118, 2738, 3434
BtgI	CCRYGG	2 cut(s)	2905, 3669
BtgZI	GCGATG	1 cut(s)	2840
BtsIMutI	CAGTG	6 cut(s)	763, 2073, 2963, 3315, 3460, 3925
BveI	ACCTGC	1 cut(s)	3718
Cac8I	GCNNGC	5 cut(s)	210, 1917, 2092, 3039, 4162
CfoI	GCGC	6 cut(s)	513, 785, 1102, 1602, 1726, 2385
Cfr10I	RCCGGY	1 cut(s)	2444
Cfr13I	GGNCC	5 cut(s)	175, 1267, 2116, 2736, 3897
CseI	GACGC	2 cut(s)	419, 3858
CsiI	ACCWGGT	2 cut(s)	226, 1498
CspAI	ACCGGT	1 cut(s)	2444
DraIII	CACNNNGTG	4 cut(s)	185, 1628, 2588, 3293
DriI	GACNNNNNGTC	2 cut(s)	1045, 3124
EaeI	YGGCCR	1 cut(s)	3432
Eam1104I	CTCTTC	6 cut(s)	1425, 1521, 1813, 2997, 3099, 4146
Eam1105I	GACNNNNNGTC	2 cut(s)	1045, 3124
EarI	CTCTTC	6 cut(s)	1425, 1521, 1813, 2997, 3099, 4146
EciI	GGCGGA	1 cut(s)	2189
Ecl136II	GAGCTC	1 cut(s)	2657
Eco105I	TACGTA	1 cut(s)	279
Eco130I	CCWWGG	2 cut(s)	2905, 3669
Eco24I	GRGCYC	2 cut(s)	2659, 4166
Eco32I	GATATC	3 cut(s)	262, 1474, 2000
Eco47I	GGWCC	2 cut(s)	1267, 3897
Eco47III	AGCGCT	2 cut(s)	1601, 1725
Eco53kI	GAGCTC	1 cut(s)	2657
Eco57I	CTGAAG	7 cut(s)	75, 105, 567, 647, 960, 1358, 3632
Eco81I	CCTNAGG	1 cut(s)	795
Eco88I	CYCGRG	2 cut(s)	1216, 1756
Eco91I	GGTNACC	1 cut(s)	3251
EcoICRI	GAGCTC	1 cut(s)	2657
EcoO109I	RGGNCCY	2 cut(s)	175, 1267
EcoO65I	GGTNACC	1 cut(s)	3251
EcoRI	GAATTC	1 cut(s)	2959
EcoRII	CCWGG	4 cut(s)	226, 1498, 3855, 4209
EcoRV	GATATC	3 cut(s)	262, 1474, 2000
EcoT14I	CCWWGG	2 cut(s)	2905, 3669
EcoT38I	GRGCYC	2 cut(s)	2659, 4166
ErhI	CCWWGG	2 cut(s)	2905, 3669
FalI	AAGNNNNNCTT	4 cut(s)	1098, 1130, 3995, 4027
FaqI	GGGAC	2 cut(s)	834, 4450
FauI	CCCGC	2 cut(s)	3646, 4153
FauNDI	CATATG	3 cut(s)	345, 3781, 4240
FbaI	TGATCA	1 cut(s)	3550
FblI	GTMKAC	2 cut(s)	319, 2031
FriOI	GRGCYC	2 cut(s)	2659, 4166
FspBI	CTAG	8 cut(s)	236, 1103, 1478, 2007, 2120, 2217, 2397, 3489
FspI	TGCGCA	1 cut(s)	2384
GlaI	GCGC	6 cut(s)	512, 784, 1101, 1601, 1725, 2384
GsaI	CCCAGC	1 cut(s)	3752
GsuI	CTGGAG	5 cut(s)	2741, 2987, 3192, 3900, 4329
HaeII	RGCGCY	3 cut(s)	1103, 1603, 1727
HaeIII	GGCC	5 cut(s)	176, 2043, 2118, 2738, 3434
HapII	CCGG	2 cut(s)	690, 2445
HgaI	GACGC	2 cut(s)	419, 3858
HhaI	GCGC	6 cut(s)	513, 785, 1102, 1602, 1726, 2385
Hin6I	GCGC	6 cut(s)	511, 783, 1100, 1600, 1724, 2383
HinP1I	GCGC	6 cut(s)	511, 783, 1100, 1600, 1724, 2383
HincII	GTYRAC	4 cut(s)	1462, 2460, 2902, 3359
HindII	GTYRAC	4 cut(s)	1462, 2460, 2902, 3359
HindIII	AAGCTT	8 cut(s)	60, 1370, 1527, 2013, 2352, 2403, 2627, 3039
HpaI	GTTAAC	1 cut(s)	2460
HpaII	CCGG	2 cut(s)	690, 2445
HphI	GGTGA	8 cut(s)	86, 107, 389, 479, 1597, 1667, 3245, 4243
Hpy99I	CGWCG	2 cut(s)	1774, 4071
HpyCH4III	ACNGT	8 cut(s)	133, 249, 619, 767, 1334, 2544, 2781, 4047
HpyCH4IV	ACGT	9 cut(s)	278, 1123, 1395, 1769, 2467, 3355, 3402, 4066, 4463
HpySE526I	ACGT	9 cut(s)	278, 1123, 1395, 1769, 2467, 3355, 3402, 4066, 4463
HspAI	GCGC	6 cut(s)	511, 783, 1100, 1600, 1724, 2383
Ksp22I	TGATCA	1 cut(s)	3550
KspAI	GTTAAC	1 cut(s)	2460
LguI	GCTCTTC	1 cut(s)	1521
MabI	ACCWGGT	2 cut(s)	226, 1498
MaeI	CTAG	8 cut(s)	236, 1103, 1478, 2007, 2120, 2217, 2397, 3489
MaeII	ACGT	9 cut(s)	278, 1123, 1395, 1769, 2467, 3355, 3402, 4066, 4463
MaeIII	GTNAC	8 cut(s)	1132, 1445, 1620, 1628, 1660, 2519, 3251, 3923
MbiI	CCGCTC	1 cut(s)	3641
MfeI	CAATTG	3 cut(s)	1257, 1844, 3385
MflI	RGATCY	6 cut(s)	29, 49, 684, 1165, 3887, 4008
MhlI	GDGCHC	6 cut(s)	2075, 2659, 3181, 3189, 4166, 4268
MluI	ACGCGT	1 cut(s)	1136
MlyI	GAGTC	8 cut(s)	587, 1016, 1268, 2286, 2527, 3134, 3931, 4379
MmeI	TCCRAC	6 cut(s)	7, 907, 3625, 3668, 4398, 4519
MroXI	GAANNNNTTC	2 cut(s)	1680, 2150
MslI	CAYNNNNRTG	3 cut(s)	333, 3366, 3512
MspA1I	CMGCKG	3 cut(s)	1181, 2719, 4288
MspCI	CTTAAG	4 cut(s)	1910, 2630, 2945, 4181
MspI	CCGG	2 cut(s)	690, 2445
MspR9I	CCNGG	5 cut(s)	228, 691, 1500, 3857, 4211
MunI	CAATTG	3 cut(s)	1257, 1844, 3385
Mva1269I	GAATGC	1 cut(s)	613
MvaI	CCWGG	4 cut(s)	228, 1500, 3857, 4211
MvnI	CGCG	2 cut(s)	727, 1138
NciI	CCSGG	1 cut(s)	691
NcoI	CCATGG	2 cut(s)	2905, 3669
NdeI	CATATG	3 cut(s)	345, 3781, 4240
NlaIV	GGNNCC	8 cut(s)	51, 177, 1167, 1269, 1872, 2892, 3523, 3861
NmuCI	GTSAC	6 cut(s)	1620, 1628, 1660, 2519, 3251, 3923
NsbI	TGCGCA	1 cut(s)	2384
NspI	RCATGY	5 cut(s)	778, 1746, 1765, 2330, 2389
PaeR7I	CTCGAG	1 cut(s)	1216
PaqCI	CACCTGC	1 cut(s)	3718
PciI	ACATGT	2 cut(s)	1742, 2326
PciSI	GCTCTTC	1 cut(s)	1521
PcsI	WCGNNNNNNNCGW	2 cut(s)	1031, 3399
PctI	GAATGC	1 cut(s)	613
PdmI	GAANNNNTTC	2 cut(s)	1680, 2150
PfeI	GAWTC	7 cut(s)	847, 1109, 1784, 3053, 3320, 3832, 3911
PflMI	CCANNNNNTGG	2 cut(s)	185, 349
PinAI	ACCGGT	1 cut(s)	2444
PleI	GAGTC	8 cut(s)	586, 1016, 1268, 2286, 2526, 3133, 3930, 4379
PpsI	GAGTC	8 cut(s)	586, 1016, 1268, 2286, 2526, 3133, 3930, 4379
Ppu21I	YACGTR	2 cut(s)	279, 2468
PpuMI	RGGWCCY	1 cut(s)	1267
PscI	ACATGT	2 cut(s)	1742, 2326
PshAI	GACNNNNGTC	1 cut(s)	134
PshBI	ATTAAT	2 cut(s)	432, 4035
Psp124BI	GAGCTC	1 cut(s)	2659
Psp1406I	AACGTT	1 cut(s)	3402
Psp5II	RGGWCCY	1 cut(s)	1267
Psp6I	CCWGG	4 cut(s)	226, 1498, 3855, 4209
PspEI	GGTNACC	1 cut(s)	3251
PspFI	CCCAGC	1 cut(s)	3748
PspGI	CCWGG	4 cut(s)	226, 1498, 3855, 4209
PspN4I	GGNNCC	8 cut(s)	51, 177, 1167, 1269, 1872, 2892, 3523, 3861
PspPI	GGNCC	5 cut(s)	175, 1267, 2116, 2736, 3897
PspPPI	RGGWCCY	1 cut(s)	1267
PsuI	RGATCY	6 cut(s)	29, 49, 684, 1165, 3887, 4008
PvuII	CAGCTG	3 cut(s)	1181, 2719, 4288
RseI	CAYNNNNRTG	3 cut(s)	333, 3366, 3512
SacI	GAGCTC	1 cut(s)	2659
SapI	GCTCTTC	1 cut(s)	1521
Sau96I	GGNCC	5 cut(s)	175, 1267, 2116, 2736, 3897
ScaI	AGTACT	2 cut(s)	937, 4371
SchI	GAGTC	8 cut(s)	587, 1016, 1268, 2286, 2527, 3134, 3931, 4379
ScrFI	CCNGG	5 cut(s)	228, 691, 1500, 3857, 4211
SduI	GDGCHC	6 cut(s)	2075, 2659, 3181, 3189, 4166, 4268
SexAI	ACCWGGT	2 cut(s)	226, 1498
SfcI	CTRYAG	1 cut(s)	2777
Sfr274I	CTCGAG	1 cut(s)	1216
SinI	GGWCC	2 cut(s)	1267, 3897
SlaI	CTCGAG	1 cut(s)	1216
SmiMI	CAYNNNNRTG	3 cut(s)	333, 3366, 3512
SmlI	CTYRAG	7 cut(s)	1216, 1910, 2630, 2831, 2945, 4181, 4514
SmoI	CTYRAG	7 cut(s)	1216, 1910, 2630, 2831, 2945, 4181, 4514
SnaBI	TACGTA	1 cut(s)	279
SpeI	ACTAGT	1 cut(s)	3488
SsiI	CCGC	5 cut(s)	1176, 2174, 3431, 3639, 4160
SspI	AATATT	1 cut(s)	456
SspMI	CTAG	8 cut(s)	236, 1103, 1478, 2007, 2120, 2217, 2397, 3489
SstI	GAGCTC	1 cut(s)	2659
StyD4I	CCNGG	5 cut(s)	226, 689, 1498, 3855, 4209
StyI	CCWWGG	2 cut(s)	2905, 3669
TaaI	ACNGT	8 cut(s)	133, 249, 619, 767, 1334, 2544, 2781, 4047
TaiI	ACGT	9 cut(s)	281, 1126, 1398, 1772, 2470, 3358, 3405, 4069, 4466
TaqI	TCGA	9 cut(s)	45, 961, 1217, 1299, 1521, 1547, 2323, 3808, 4388
TaqII	GACCGA	2 cut(s)	2049, 3885
TatI	WGTACW	9 cut(s)	935, 1212, 3161, 3880, 4079, 4227, 4290, 4301, 4369
TauI	GCSGC	2 cut(s)	1178, 3434
TfiI	GAWTC	7 cut(s)	847, 1109, 1784, 3053, 3320, 3832, 3911
TscAI	CASTG	6 cut(s)	770, 2080, 2970, 3322, 3467, 3932
TseFI	GTSAC	6 cut(s)	1620, 1628, 1660, 2519, 3251, 3923
Tsp45I	GTSAC	6 cut(s)	1620, 1628, 1660, 2519, 3251, 3923
TspGWI	ACGGA	3 cut(s)	453, 1883, 2264
TspRI	CASTG	6 cut(s)	770, 2080, 2970, 3322, 3467, 3932
Van91I	CCANNNNNTGG	2 cut(s)	185, 349
Vha464I	CTTAAG	4 cut(s)	1910, 2630, 2945, 4181
VpaK11BI	GGWCC	2 cut(s)	1267, 3897
VspI	ATTAAT	2 cut(s)	432, 4035
XapI	RAATTY	8 cut(s)	998, 1454, 2024, 2959, 3110, 3138, 3979, 4233
XceI	RCATGY	5 cut(s)	778, 1746, 1765, 2330, 2389
XcmI	CCANNNNNNNNNTGG	1 cut(s)	3924
XhoI	CTCGAG	1 cut(s)	1216
XmiI	GTMKAC	2 cut(s)	319, 2031
XmnI	GAANNNNTTC	2 cut(s)	1680, 2150
XspI	CTAG	8 cut(s)	236, 1103, 1478, 2007, 2120, 2217, 2397, 3489
ZrmI	AGTACT	2 cut(s)	937, 4371

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.