Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr10

Orthogroup ID

OG0003289

Physical Location & Seq

Genomic Coordinates Reverse (-)

26548887 .. 26560721

Gene Structure 11835 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD10G1172900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 4536 bp

ATGGCTGCTGCTATTAGTCTTGTGGTTGGATCTCTTGTTTGGGTCGAGGATCCTGAAGAAGCTTGGCTAGATGGTGAAATTGTGGAGATTAAAAGTGAAAACTACAAGGTTCTCTGCACTTCAGGGAAGACGGTTGTCGTTAAAGCCTCCAGTGTCTATCCTAAAGATGCCGAGGCCCCACCCTGTGGAGTGGATGATATGACAAAGCTGGCTTATTTGCATGAACCAGGTGTCCTGGATAATTTAAGATCAAGATATGATATCAATGAAATATATACTTACACAGGGAGCATTTTGATCGCCGTGAACCCTTTTTGTAGACTACCTCATCTGTATGATAGCCATATGATGGAACAATATAAAGGGGCAGATTTTGGTGAACTGAGCCCACACCCGTTTGCTGTTGCAGACGAGGCATATAGACTTATGATTAATGACGGAATAAGTCAGTCAATATTGGTTAGTGGTGAGAGTGGGGCTGGTAAAACTGAAAGCACCAAATTGCTTATGCGCTATCTTGCCTACATGGGAGGGAGAGGTGTTGCTGAAGGGAGGAGTGTTGAGCAGCAAGTCTTGGAGTCAAATCCTGTTTTAGAAGCATTTGGAAATGCGAAGACCGTTAGAAACAATAACTCCAGTCGTTTTGGTAAGTTTGTGGAGCTTCAGTTTGATCAGAATGGGAGGATCTCTGGAGCTGCAATAAGAACTTATTTGCTTGAAAGATCACGCGTTTGTCAGGTGTCCAATCCTGAGAGAAATTATCATTGTTTCTACATGCTTTGTGCTGCACCACCTGAGGATGTTCAGAGGTTCAAATTGGGACCCGCAAAAACGTTTCATTATTTGAATCAATCAGATTGTATTGAATTGGATGCGCTTGATGATGCTGAAGAATACATTGCTACAAGGAAGGCAATGGAGGTTGTTGGAATAAGTTCTGAAGAACAGGATGCCATATTTCGAGTTGTGGCAGCAATTCTACATCTTGGAAACATTGAATTTGCAAAGGGTAAGGAAATGGACTCTTCCATGCCGAAGGACGACAAATCTTGGTTCCACCTAAAAACTGCTGCCCAGCTTTTCATGTGTGATGTGAAGGCGCTAGAAGATTCTCTTTGCAAACGCATTATCGTTACTCGTGATGAAACAATCACAAAATGGCTGGATCCAGAAGCCGCAGCTGTCAGTAGAGATGCTTTGGCTAAAGTTGTGTACTCGAGGTTGTTTGATTGGCTTGTGGATAAGATTAACAATTCAATTGGTCAAGATCCTGACTCAAAGTTCTTAATTGGAGTTTTGGACATTTATGGATTTGAGAGTTTCAAAACGAACAGTTTTGAGCAATTTTGCATCAATTTGACGAATGAGAAGCTTCAGCAACATTTCAATCAGCACGTTTTCAAAATGGAGCAAGAAGAATATACAAAAGAAGAGATTGATTGGAGTTACATTGAATTTGTTGACAACAAAGATATCCTAGATCTGATTGAAAAGAAACCTGGTGGCATAATTGCTCTTCTCGATGAAGCTTGTATGTTTCCAAGATCGACACATGAAACATTTGCTCAGAAGTTATATCAAACATTCAAAAATCATAAGCGCTTCATGAAACCAAAATTGTCTCAAAGTGACTTCACAATTTGCCATTATGCTGGTGATGTCACTTATCAAACTGAACTGTTTCTGGACAAGAACAAGGACTATGTGGTTGCTGAGCATCAAGCACTTTTGAGTGCTTCTACATGTTCCTTTGTCTCGGGCATGTTTACGTCTTTAATTGAGGATTCGTCCAAGTCGTCAAAGTTTTCCTCAATAGGTTCTCGGTTTAAGCAACAACTGCAACAATTGCTCGAAACTCTCAGTTCTACGGAGCCACATTATATTCGGTGTGTAAAACCCAACAATGTTCTTAAGCCAGCAATCTTTGAGAACAAAAATGTTTTACAACAACTTCGGTGCGGGGGGGTCATGGAAGCAATCAGGATAAGCTGTGCTGGGTATCCTACTAGAAAAGCTTTTGCTGAATTTATAGACCGATTTGGCCTCCTTGCACCTGAAGTTTTGGATGGAAGCACTGATGAGGTCAATGCTTGCAAGAGACTTTTAGAGAAGGTGGGCCTAGAAGGCTATCAGATTGGTAAAACAAAGGTCTTCCTTCGGGCTGGTCAGATGGCGGAACTGGATGCTCGTAGAAGTGAGGTCTTAGGAAAATCAGCTAGTATTATTCAAAGGAAAGTGCGTTCTTATTTGGCTAAAAGAAGTTTTATCGTGCTCCGTCTTTCTGCAATACGACTTCAAGCTGCATGCAGAGGACATCTTGCTCGACATGTCTATCAGGGCATGAGGAGAGAAGCTTCCAGTCTAATGATCCAAAGGCATTTGCGCATGTATCTTGCTAGGAAAGCTTACAAAGAATTGTATTGCTCTGCTGTATCTATTCAGACCGGTATGCGAGGGTTAACTGCCCGTAATGAGCTACGCTTCAGAAGACAGACCAAAGCAGCAATTATCATCCAGAGCCACTGTCGCAAATTCTTGGCATGGTCGCATTATAAGAAGACAAAGAAAGCTGCAATTGCCACACAATGTGCTTGGAGGGGAAAGGTTGCTCGTATGGAACTACGAAAGCTTAAGATGGCTGCAAGGGAGACGGGAGCTCTCCAAGCTGCCAAAAATAAATTAGAAAAGCAAGTTGAAGAATTGACATGGAGATTACAGCTGGAGAAACGAATGAGGGCTGACATGGAAGAAGCCAAAACACAAGAAAATGAAAAACTACAGTCTGCATTGCAAGAGATGCAAGTTCAATTTAAAGAAACTAAGGCGATGCTTGAGAAGGAGTGTGAAGCCTTAGAAAGAGCTGAAAAAGTAGTCCCATTAATACAGGAGGTTCCAGTTATTGACCATGGCTTGATGGAGAAGCTAACCAACGAAAACGAACAACTTAAGGCTTTAGTGAATTCATTGGAGATAAAAATTGATGAAACAGAAAAGAAATATGAAGAGGCAAACAAAATGAGTGAAGAAAGGTTGAAGCAAGCTTTGGAGGCAGAATCACAGATTATTAAGTTAAAGACAAACATGCAAAGGTTATTTGAGCTTGAAGAGAAATTTGCTGACATTGAGTCTGAAAACAAAATTCTTCGGCGACAGCAAATAAGTACACCTGTTAAAAGGGCACATGAGCACCCACCAATTCCACCAATTCCAGAAACTCAGAGAGTGGAAAATGGTCACCATGTGAACGAAGAGAACAGAGCCAATGAACCACAAAGTGCAACGCCAAAGAAGTTTGGCACCGAATCTGATAGCAAGCTGAGGAGATCATTTGTTGAACGTCAACATGAGAGTGTTGATGCTCTTATCAACTGTGTCGTGAAAAACATTGGGTTCAGTCAAGGAAAGCCTGTTGCGGCCTTTACCATCTACAAATGTCTTCTCCACTGGAAATCTTTTGAGGCTGAAAGGACTAGTGTGTTTGATCGCCTCATCCAGATGATTGGTTCGGAAATTGAGAACCAAGACAACAATGATCACATGGCTTACTGGCTGTCAAATACATCTGCGTTATTGTTCTTACTCCAACGAAGTCTGAAGGGTTCTGGAGCAGGTGCAAAGAAGCCATCCGCTCCAACATCTCTCTTTGGGAGGATGACCATGGGTTTTCGCTCATCCCCTTCTTCTGCAAACCTTGCACCTGCACTTGAGGTAGTACGCCAAGTGGAGGCCAAGTACCCTGCATTGCTATTCAAGCAGCAGCTTACAGCATATGTGGAGAAAATATATGGCATTATTCGAGACAACTTGAAAAAGGAGTTGAATCCATTGCTTTCTTTGTGCATCCAGGCACCGAGAATGTCAAAGGGTGTACTAAGATCTGGGCGGTCCTTTGGGAAAGATTCTCCAGCGAGTCACTGGCTGAGCATTATTGACGGCCTTAGCACTTTCCTTAGCACATTGAAAGAAAATTTTGTGCCTCCGGTTCTTGTCAAGGAGATCTTCACTCAGACTTTCTCATATATTAATGTACAACTGTTCAATAGTCTTCTCCTACGCCGTGAATGCTGTACATTCAGCAATGGGGAGTATGTGAAATCTGGATTGGCTGAGTTGGAGTTGTGGTGTTGTCAAGCAAAAGAAGAGTATGCGGGCTCATCGTGGGATGAACTTAAGCACATAAGACAAGCTGTTGGTTTCCTGGTCATACATCAAAAGTATAGAATTTCTTATGATGAAATCACCAATGATCTGTGCCCCATCCTGAGTGTTCAACAGCTGTATAGAATATGTACTCTCTATTGGGATGACAACTATAATACCCGAAGTGTATCTCCAGATGTAATTTCCAGCATGAGAGTACTTATGACTGAGGACTCCAACAATGCTGTCAGCAACTCATTTTTGTTGGATGACAATTCCAGCATCCCCTTCTCGGTTGATGACCTCTCTACGTCCCTACAAGAGAAGGAGTTTTCAGATGTCCAACCAGCAGATGAACTTCTTGAGCATCCCGCCTTCGAGTTTTTACATGAGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000146 GO:0000166 GO:0000331 GO:0003674 GO:0003774 GO:0003779 GO:0003824 GO:0005488 GO:0005515 GO:0005524 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005856 GO:0006928 GO:0008092 GO:0008144 GO:0008150 GO:0009987 GO:0015629 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017076 GO:0017111 GO:0030029 GO:0030048 GO:0030554 GO:0030898 GO:0031410 GO:0031982 GO:0032553 GO:0032555 GO:0032559 GO:0033275 GO:0035639 GO:0036094 GO:0042623 GO:0042641 GO:0043167 GO:0043168 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043531 GO:0044422 GO:0044424 GO:0044430 GO:0044444 GO:0044446 GO:0044464 GO:0044877 GO:0051015 GO:0051179 GO:0051234 GO:0051640 GO:0051641 GO:0051656 GO:0070252 GO:0097159 GO:0097367 GO:0097708 GO:0140027 GO:1901265 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DIL IQ Myosin_N Myosin_head

Protein Analysis

1512

Amino Acids

171.82

Weight (kDa)

6.75

Isoelectric Point (pI)

48.1

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Myosin_N	PF02736	9 - 52	2.9e-14	Myosin N-terminal SH3-like domain
Myosin_head	PF00063	64 - 720	1.9e-254	Myosin head (motor domain)
DIL	PF01843	1333 - 1437	1.3e-29	DIL domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0003289)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G04160 AT1G04160 AT2G20290 AT2G20290 AT2G20290 AT2G20290 AT4G28710 AT5G43900 AT5G43900 AT5G43900
fragaria_vesca	FvH4_2g20160 FvH4_2g20160 FvH4_2g20160 FvH4_2g20160 FvH4_2g20160 FvH4_2g20160 FvH4_2g20160 FvH4_2g20160
malus_domestica	MD05G1184900.v1.1 MD10G1172900.v1.1
prunus_persica	Prupe.8G211100_v2.0.a1 Prupe.8G211100_v2.0.a1
pyrus_communis	pycom05g16970 pycom10g14900
rosa_chinensis	RchiOBHm_Chr6g0285891
rosa_laevigata	RLG00000012601
rosa_multiflora	Rmu_sc0004808.1_g000001
rosa_roxburghii	Rroxscaffold_7G00182630 Rroxscaffold_7G00183900 Rroxscaffold_7G00184180
rosa_rugosa	Rorug06G0177400
rosa_samantha	Rh6AG289400 Rh6BG291200 Rh6CG292200 Rh6DG284300
rosa_wichuraiana	Rw6G024930

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	2553
AarI	CACCTGC	2 cut(s)	3599, 3706
Acc16I	TGCGCA	1 cut(s)	2384
Acc36I	ACCTGC	2 cut(s)	3599, 3706
AccB1I	GGYRCC	2 cut(s)	3296, 3847
AccB7I	CCANNNNNTGG	2 cut(s)	185, 349
AccBSI	CCGCTC	1 cut(s)	3629
AccI	GTMKAC	1 cut(s)	319
AccII	CGCG	1 cut(s)	729
AciI	CCGC	9 cut(s)	825, 1176, 1959, 2174, 3413, 3627, 3883, 4148, 4512
AclI	AACGTT	1 cut(s)	833
AclWI	GGATC	8 cut(s)	37, 44, 57, 692, 1160, 1173, 1262, 2362
AcsI	RAATTY	9 cut(s)	998, 1454, 2024, 2531, 2959, 3110, 3138, 3967, 4221
AdeI	CACNNNGTG	4 cut(s)	185, 2588, 3241, 3275
AfaI	GTAC	9 cut(s)	1214, 3163, 3714, 3734, 3870, 4029, 4069, 4291, 4359
AfeI	AGCGCT	1 cut(s)	1601
AfiI	CCNNNNNNNGG	4 cut(s)	185, 349, 3724, 4432
AflII	CTTAAG	4 cut(s)	1910, 2630, 2945, 4169
AflIII	ACRYGT	3 cut(s)	727, 1742, 2326
AgeI	ACCGGT	1 cut(s)	2444
AhdI	GACNNNNNGTC	1 cut(s)	3124
AhlI	ACTAGT	1 cut(s)	3470
AjnI	CCWGG	5 cut(s)	226, 234, 1498, 3843, 4197
AjuI	GAANNNNNNNTTGG	4 cut(s)	3026, 3058, 4085, 4117
AloI	GAACNNNNNNTCC	4 cut(s)	216, 248, 2224, 2256
Alw21I	GWGCWC	3 cut(s)	2274, 2659, 3189
Alw26I	GTCTC	5 cut(s)	1626, 1759, 2092, 2642, 3792
AlwI	GGATC	8 cut(s)	37, 44, 57, 692, 1160, 1173, 1262, 2362
Ama87I	CYCGRG	2 cut(s)	1216, 1756
Aor51HI	AGCGCT	1 cut(s)	1601
AoxI	GGCC	6 cut(s)	174, 2041, 2116, 3414, 3726, 3934
ApoI	RAATTY	9 cut(s)	998, 1454, 2024, 2531, 2959, 3110, 3138, 3967, 4221
AseI	ATTAAT	3 cut(s)	432, 2879, 4023
AsiGI	ACCGGT	1 cut(s)	2444
Asp700I	GAANNNNTTC	2 cut(s)	934, 1680
AspLEI	GCGC	5 cut(s)	513, 877, 1102, 1602, 2385
AspS9I	GGNCC	4 cut(s)	175, 821, 2116, 3885
AsuHPI	GGTGA	6 cut(s)	86, 389, 479, 1667, 3227, 4231
AvaI	CYCGRG	2 cut(s)	1216, 1756
AvaII	GGWCC	2 cut(s)	821, 3885
AxyI	CCTNAGG	1 cut(s)	795
BaeGI	GKGCMC	2 cut(s)	3181, 4256
BamHI	GGATCC	2 cut(s)	49, 1165
BanI	GGYRCC	2 cut(s)	3296, 3847
BanII	GRGCYC	3 cut(s)	389, 2659, 4154
BauI	CACGAG	1 cut(s)	1137
BbsI	GAAGAC	7 cut(s)	134, 620, 2143, 2494, 2564, 3428, 4037
Bbv12I	GWGCWC	3 cut(s)	2274, 2659, 3189
BbvCI	CCTCAGC	1 cut(s)	3317
BccI	CCATC	9 cut(s)	65, 343, 2060, 2164, 2629, 2908, 3431, 3631, 4265
BceAI	ACGGC	3 cut(s)	287, 3949, 4041
BcgI	CGANNNNNNTGC	2 cut(s)	280, 314
BciT130I	CCWGG	5 cut(s)	228, 236, 1500, 3845, 4199
BciVI	GTATCC	1 cut(s)	2010
BclI	TGATCA	2 cut(s)	670, 3532
BcoDI	GTCTC	5 cut(s)	1626, 1759, 2092, 2642, 3792
BcuI	ACTAGT	1 cut(s)	3470
BfaI	CTAG	8 cut(s)	68, 1103, 1478, 2007, 2120, 2217, 2397, 3471
BfmI	CTRYAG	1 cut(s)	2777
BfoI	RGCGCY	2 cut(s)	1103, 1603
BfrI	CTTAAG	4 cut(s)	1910, 2630, 2945, 4169
BfuAI	ACCTGC	2 cut(s)	3599, 3706
BfuI	GTATCC	1 cut(s)	2010
BglI	GCCNNNNNGGC	1 cut(s)	2124
BglII	AGATCT	3 cut(s)	1480, 3875, 3996
BlpI	GCTNAGC	2 cut(s)	1713, 3920
BmcAI	AGTACT	1 cut(s)	4359
Bme1390I	CCNGG	5 cut(s)	228, 236, 1500, 3845, 4199
Bme18I	GGWCC	2 cut(s)	821, 3885
BmeRI	GACNNNNNGTC	1 cut(s)	3124
BmeT110I	CYCGRG	2 cut(s)	1216, 1756
BmgT120I	GGNCC	4 cut(s)	175, 821, 2116, 3885
BmrFI	CCNGG	5 cut(s)	228, 236, 1500, 3845, 4199
BoxI	GACNNNNGTC	1 cut(s)	134
BpiI	GAAGAC	7 cut(s)	134, 620, 2143, 2494, 2564, 3428, 4037
BpmI	CTGGAG	7 cut(s)	133, 619, 711, 2741, 3624, 3888, 4317
Bpu10I	CCTNAGC	3 cut(s)	3317, 3938, 3950
Bpu1102I	GCTNAGC	2 cut(s)	1713, 3920
BpuEI	CTTGAG	3 cut(s)	2852, 3725, 4523
BsaJI	CCNNGG	3 cut(s)	171, 2905, 3657
BsaWI	WCCGGW	2 cut(s)	2444, 3979
BsaXI	ACNNNNNCTCC	8 cut(s)	617, 647, 650, 680, 3716, 3746, 4106, 4136
Bsc4I	CCNNNNNNNGG	4 cut(s)	185, 349, 3724, 4432
Bse118I	RCCGGY	1 cut(s)	2444
Bse1I	ACTGG	8 cut(s)	150, 636, 2184, 2358, 2894, 3449, 3551, 3920
Bse21I	CCTNAGG	1 cut(s)	795
Bse3DI	GCAATG	6 cut(s)	897, 921, 2786, 3740, 3824, 4084
BseBI	CCWGG	5 cut(s)	228, 236, 1500, 3845, 4199
BseDI	CCNNGG	3 cut(s)	171, 2905, 3657
BseLI	CCNNNNNNNGG	4 cut(s)	185, 349, 3724, 4432
BseMI	GCAATG	6 cut(s)	897, 921, 2786, 3740, 3824, 4084
BseNI	ACTGG	8 cut(s)	150, 636, 2184, 2358, 2894, 3449, 3551, 3920
BseRI	GAGGAG	3 cut(s)	568, 2359, 3334
BseSI	GKGCMC	2 cut(s)	3181, 4256
BseYI	CCCAGC	2 cut(s)	1074, 1994
BsgI	GTGCAG	3 cut(s)	100, 771, 3684
Bsh1236I	CGCG	1 cut(s)	729
BshFI	GGCC	6 cut(s)	176, 2043, 2118, 3416, 3728, 3936
BshNI	GGYRCC	2 cut(s)	3296, 3847
BshTI	ACCGGT	1 cut(s)	2444
BsiHKAI	GWGCWC	3 cut(s)	2274, 2659, 3189
BsiHKCI	CYCGRG	2 cut(s)	1216, 1756
BsiSI	CCGG	2 cut(s)	2445, 3980
BslFI	GGGAC	3 cut(s)	834, 2858, 4438
BslI	CCNNNNNNNGG	4 cut(s)	185, 349, 3724, 4432
BsmAI	GTCTC	5 cut(s)	1626, 1759, 2092, 2642, 3792
BsmBI	CGTCTC	1 cut(s)	2642
BsmFI	GGGAC	3 cut(s)	834, 2858, 4438
BsmI	GAATGC	1 cut(s)	4067
BsnI	GGCC	6 cut(s)	176, 2043, 2118, 3416, 3728, 3936
BsoBI	CYCGRG	2 cut(s)	1216, 1756
Bsp1286I	GDGCHC	7 cut(s)	389, 2274, 2659, 3181, 3189, 4154, 4256
Bsp1407I	TGTACA	2 cut(s)	4027, 4067
Bsp1720I	GCTNAGC	2 cut(s)	1713, 3920
Bsp19I	CCATGG	2 cut(s)	2905, 3657
BspACI	CCGC	9 cut(s)	825, 1176, 1959, 2174, 3413, 3627, 3883, 4148, 4512
BspANI	GGCC	6 cut(s)	176, 2043, 2118, 3416, 3728, 3936
BspFNI	CGCG	1 cut(s)	729
BspHI	TCATGA	1 cut(s)	1605
BspMI	ACCTGC	2 cut(s)	3599, 3706
BspPI	GGATC	8 cut(s)	37, 44, 57, 692, 1160, 1173, 1262, 2362
BspQI	GCTCTTC	1 cut(s)	1521
BspT107I	GGYRCC	2 cut(s)	3296, 3847
BspTI	CTTAAG	4 cut(s)	1910, 2630, 2945, 4169
BsrBI	CCGCTC	1 cut(s)	3629
BsrDI	GCAATG	6 cut(s)	897, 921, 2786, 3740, 3824, 4084
BsrFI	RCCGGY	1 cut(s)	2444
BsrGI	TGTACA	2 cut(s)	4027, 4067
BsrI	ACTGG	8 cut(s)	150, 636, 2184, 2358, 2894, 3449, 3551, 3920
BssAI	RCCGGY	1 cut(s)	2444
BssECI	CCNNGG	3 cut(s)	171, 2905, 3657
BssSI	CACGAG	1 cut(s)	1137
BssT1I	CCWWGG	2 cut(s)	2905, 3657
Bst2BI	CACGAG	1 cut(s)	1137
Bst2UI	CCWGG	5 cut(s)	228, 236, 1500, 3845, 4199
Bst4CI	ACNGT	8 cut(s)	133, 619, 1334, 1680, 2525, 2781, 3371, 4035
Bst6I	CTCTTC	7 cut(s)	1030, 1425, 1521, 2997, 3099, 3243, 4134
BstAFI	CTTAAG	4 cut(s)	1910, 2630, 2945, 4169
BstAPI	GCANNNNNTGC	4 cut(s)	1837, 1846, 2797, 3692
BstAUI	TGTACA	2 cut(s)	4027, 4067
BstC8I	GCNNGC	7 cut(s)	210, 1917, 2092, 2305, 3039, 3314, 4150
BstDSI	CCRYGG	2 cut(s)	2905, 3657
BstEII	GGTNACC	1 cut(s)	3233
BstFNI	CGCG	1 cut(s)	729
BstH2I	RGCGCY	2 cut(s)	1103, 1603
BstHHI	GCGC	5 cut(s)	513, 877, 1102, 1602, 2385
BstMAI	GTCTC	5 cut(s)	1626, 1759, 2092, 2642, 3792
BstNI	CCWGG	5 cut(s)	228, 236, 1500, 3845, 4199
BstNSI	RCATGY	7 cut(s)	778, 1746, 1765, 2307, 2330, 2389, 3085
BstPAI	GACNNNNGTC	1 cut(s)	134
BstPI	GGTNACC	1 cut(s)	3233
BstSCI	CCNGG	5 cut(s)	226, 234, 1498, 3843, 4197
BstSFI	CTRYAG	1 cut(s)	2777
BstSLI	GKGCMC	2 cut(s)	3181, 4256
BstUI	CGCG	1 cut(s)	729
BstV2I	GAAGAC	7 cut(s)	134, 620, 2143, 2494, 2564, 3428, 4037
BstX2I	RGATCY	8 cut(s)	29, 49, 684, 1165, 1267, 1480, 3875, 3996
BstXI	CCANNNNNNTGG	2 cut(s)	1652, 3500
BstYI	RGATCY	8 cut(s)	29, 49, 684, 1165, 1267, 1480, 3875, 3996
Bsu36I	CCTNAGG	1 cut(s)	795
BsuI	GTATCC	1 cut(s)	2010
BsuRI	GGCC	6 cut(s)	176, 2043, 2118, 3416, 3728, 3936
BtgI	CCRYGG	2 cut(s)	2905, 3657
BtgZI	GCGATG	1 cut(s)	2840
BtsIMutI	CAGTG	5 cut(s)	157, 2073, 2521, 3442, 3913
BveI	ACCTGC	2 cut(s)	3599, 3706
Cac8I	GCNNGC	7 cut(s)	210, 1917, 2092, 2305, 3039, 3314, 4150
CciI	TCATGA	1 cut(s)	1605
CfoI	GCGC	5 cut(s)	513, 877, 1102, 1602, 2385
Cfr10I	RCCGGY	1 cut(s)	2444
Cfr13I	GGNCC	4 cut(s)	175, 821, 2116, 3885
CsiI	ACCWGGT	2 cut(s)	226, 1498
Csp6I	GTAC	9 cut(s)	1213, 3162, 3713, 3733, 3869, 4028, 4068, 4290, 4358
CspAI	ACCGGT	1 cut(s)	2444
CviQI	GTAC	9 cut(s)	1213, 3162, 3713, 3733, 3869, 4028, 4068, 4290, 4358
DraI	TTTAAA	1 cut(s)	2812
DraIII	CACNNNGTG	4 cut(s)	185, 2588, 3241, 3275
DriI	GACNNNNNGTC	1 cut(s)	3124
Eam1104I	CTCTTC	7 cut(s)	1030, 1425, 1521, 2997, 3099, 3243, 4134
Eam1105I	GACNNNNNGTC	1 cut(s)	3124
EarI	CTCTTC	7 cut(s)	1030, 1425, 1521, 2997, 3099, 3243, 4134
EciI	GGCGGA	1 cut(s)	2189
Ecl136II	GAGCTC	1 cut(s)	2657
Eco130I	CCWWGG	2 cut(s)	2905, 3657
Eco24I	GRGCYC	3 cut(s)	389, 2659, 4154
Eco32I	GATATC	2 cut(s)	262, 1474
Eco47I	GGWCC	2 cut(s)	821, 3885
Eco47III	AGCGCT	1 cut(s)	1601
Eco53kI	GAGCTC	1 cut(s)	2657
Eco81I	CCTNAGG	1 cut(s)	795
Eco88I	CYCGRG	2 cut(s)	1216, 1756
Eco91I	GGTNACC	1 cut(s)	3233
EcoICRI	GAGCTC	1 cut(s)	2657
EcoO109I	RGGNCCY	2 cut(s)	175, 821
EcoO65I	GGTNACC	1 cut(s)	3233
EcoRI	GAATTC	1 cut(s)	2959
EcoRII	CCWGG	5 cut(s)	226, 234, 1498, 3843, 4197
EcoRV	GATATC	2 cut(s)	262, 1474
EcoT14I	CCWWGG	2 cut(s)	2905, 3657
EcoT38I	GRGCYC	3 cut(s)	389, 2659, 4154
ErhI	CCWWGG	2 cut(s)	2905, 3657
Esp3I	CGTCTC	1 cut(s)	2642
FalI	AAGNNNNNCTT	4 cut(s)	1098, 1130, 3983, 4015
FaqI	GGGAC	3 cut(s)	834, 2858, 4438
FauI	CCCGC	4 cut(s)	832, 1952, 4141, 4519
FauNDI	CATATG	2 cut(s)	345, 3769
FbaI	TGATCA	2 cut(s)	670, 3532
FblI	GTMKAC	1 cut(s)	319
FriOI	GRGCYC	3 cut(s)	389, 2659, 4154
FspBI	CTAG	8 cut(s)	68, 1103, 1478, 2007, 2120, 2217, 2397, 3471
FspI	TGCGCA	1 cut(s)	2384
GlaI	GCGC	5 cut(s)	512, 876, 1101, 1601, 2384
GsaI	CCCAGC	2 cut(s)	1078, 1998
GsuI	CTGGAG	7 cut(s)	133, 619, 711, 2741, 3624, 3888, 4317
HaeII	RGCGCY	2 cut(s)	1103, 1603
HaeIII	GGCC	6 cut(s)	176, 2043, 2118, 3416, 3728, 3936
HapII	CCGG	2 cut(s)	2445, 3980
HhaI	GCGC	5 cut(s)	513, 877, 1102, 1602, 2385
Hin6I	GCGC	5 cut(s)	511, 875, 1100, 1600, 2383
HinP1I	GCGC	5 cut(s)	511, 875, 1100, 1600, 2383
HincII	GTYRAC	3 cut(s)	1462, 2460, 3341
HindII	GTYRAC	3 cut(s)	1462, 2460, 3341
HindIII	AAGCTT	8 cut(s)	60, 1370, 1527, 2013, 2352, 2403, 2627, 3039
HpaI	GTTAAC	1 cut(s)	2460
HpaII	CCGG	2 cut(s)	2445, 3980
HphI	GGTGA	6 cut(s)	86, 389, 479, 1667, 3227, 4231
HpyCH4III	ACNGT	8 cut(s)	133, 619, 1334, 1680, 2525, 2781, 3371, 4035
HpyCH4IV	ACGT	5 cut(s)	833, 1395, 1769, 3337, 4451
HpySE526I	ACGT	5 cut(s)	833, 1395, 1769, 3337, 4451
HspAI	GCGC	5 cut(s)	511, 875, 1100, 1600, 2383
KflI	GGGWCCC	1 cut(s)	821
Ksp22I	TGATCA	2 cut(s)	670, 3532
KspAI	GTTAAC	1 cut(s)	2460
LguI	GCTCTTC	1 cut(s)	1521
LmnI	GCTCC	9 cut(s)	288, 658, 692, 1408, 1870, 2277, 2654, 3605, 3634
MabI	ACCWGGT	2 cut(s)	226, 1498
MaeI	CTAG	8 cut(s)	68, 1103, 1478, 2007, 2120, 2217, 2397, 3471
MaeII	ACGT	5 cut(s)	833, 1395, 1769, 3337, 4451
MaeIII	GTNAC	6 cut(s)	1132, 1445, 1628, 1660, 3233, 3911
MbiI	CCGCTC	1 cut(s)	3629
MfeI	CAATTG	3 cut(s)	1257, 1844, 2574
MflI	RGATCY	8 cut(s)	29, 49, 684, 1165, 1267, 1480, 3875, 3996
MhlI	GDGCHC	7 cut(s)	389, 2274, 2659, 3181, 3189, 4154, 4256
MluI	ACGCGT	1 cut(s)	727
MlyI	GAGTC	6 cut(s)	587, 1016, 1268, 3134, 3919, 4367
MmeI	TCCRAC	8 cut(s)	7, 907, 3607, 3656, 4092, 4386, 4401, 4507
MroXI	GAANNNNTTC	2 cut(s)	934, 1680
MslI	CAYNNNNRTG	3 cut(s)	333, 3348, 3494
MspA1I	CMGCKG	3 cut(s)	1181, 2719, 4276
MspCI	CTTAAG	4 cut(s)	1910, 2630, 2945, 4169
MspI	CCGG	2 cut(s)	2445, 3980
MspR9I	CCNGG	5 cut(s)	228, 236, 1500, 3845, 4199
MunI	CAATTG	3 cut(s)	1257, 1844, 2574
Mva1269I	GAATGC	1 cut(s)	4067
MvaI	CCWGG	5 cut(s)	228, 236, 1500, 3845, 4199
MvnI	CGCG	1 cut(s)	729
NcoI	CCATGG	2 cut(s)	2905, 3657
NdeI	CATATG	2 cut(s)	345, 3769
NmeAIII	GCCGAG	1 cut(s)	196
NmuCI	GTSAC	4 cut(s)	1628, 1660, 3233, 3911
NsbI	TGCGCA	1 cut(s)	2384
NspI	RCATGY	7 cut(s)	778, 1746, 1765, 2307, 2330, 2389, 3085
PaeI	GCATGC	1 cut(s)	2307
PaeR7I	CTCGAG	1 cut(s)	1216
PagI	TCATGA	1 cut(s)	1605
PaqCI	CACCTGC	2 cut(s)	3599, 3706
PciI	ACATGT	2 cut(s)	1742, 2326
PciSI	GCTCTTC	1 cut(s)	1521
PcsI	WCGNNNNNNNCGW	1 cut(s)	1793
PctI	GAATGC	1 cut(s)	4067
PdmI	GAANNNNTTC	2 cut(s)	934, 1680
PfeI	GAWTC	7 cut(s)	847, 1109, 1784, 3053, 3302, 3820, 3899
PflMI	CCANNNNNTGG	2 cut(s)	185, 349
PfoI	TCCNGGA	1 cut(s)	234
PinAI	ACCGGT	1 cut(s)	2444
PleI	GAGTC	6 cut(s)	586, 1016, 1268, 3133, 3918, 4367
PpsI	GAGTC	6 cut(s)	586, 1016, 1268, 3133, 3918, 4367
PpuMI	RGGWCCY	1 cut(s)	821
PscI	ACATGT	2 cut(s)	1742, 2326
PshAI	GACNNNNGTC	1 cut(s)	134
PshBI	ATTAAT	3 cut(s)	432, 2879, 4023
PsiI	TTATAA	1 cut(s)	2553
Psp124BI	GAGCTC	1 cut(s)	2659
Psp1406I	AACGTT	1 cut(s)	833
Psp5II	RGGWCCY	1 cut(s)	821
Psp6I	CCWGG	5 cut(s)	226, 234, 1498, 3843, 4197
PspEI	GGTNACC	1 cut(s)	3233
PspFI	CCCAGC	2 cut(s)	1074, 1994
PspGI	CCWGG	5 cut(s)	226, 234, 1498, 3843, 4197
PspPI	GGNCC	4 cut(s)	175, 821, 2116, 3885
PspPPI	RGGWCCY	1 cut(s)	821
PspXI	VCTCGAGB	1 cut(s)	1216
PsuI	RGATCY	8 cut(s)	29, 49, 684, 1165, 1267, 1480, 3875, 3996
PvuII	CAGCTG	3 cut(s)	1181, 2719, 4276
RsaI	GTAC	9 cut(s)	1214, 3163, 3714, 3734, 3870, 4029, 4069, 4291, 4359
RsaNI	GTAC	9 cut(s)	1213, 3162, 3713, 3733, 3869, 4028, 4068, 4290, 4358
RseI	CAYNNNNRTG	3 cut(s)	333, 3348, 3494
SacI	GAGCTC	1 cut(s)	2659
SapI	GCTCTTC	1 cut(s)	1521
Sau96I	GGNCC	4 cut(s)	175, 821, 2116, 3885
ScaI	AGTACT	1 cut(s)	4359
SchI	GAGTC	6 cut(s)	587, 1016, 1268, 3134, 3919, 4367
ScrFI	CCNGG	5 cut(s)	228, 236, 1500, 3845, 4199
SduI	GDGCHC	7 cut(s)	389, 2274, 2659, 3181, 3189, 4154, 4256
SexAI	ACCWGGT	2 cut(s)	226, 1498
SfcI	CTRYAG	1 cut(s)	2777
Sfr274I	CTCGAG	1 cut(s)	1216
SinI	GGWCC	2 cut(s)	821, 3885
SlaI	CTCGAG	1 cut(s)	1216
SmiMI	CAYNNNNRTG	3 cut(s)	333, 3348, 3494
SmlI	CTYRAG	8 cut(s)	1216, 1910, 2630, 2831, 2945, 3704, 4169, 4502
SmoI	CTYRAG	8 cut(s)	1216, 1910, 2630, 2831, 2945, 3704, 4169, 4502
SpeI	ACTAGT	1 cut(s)	3470
SphI	GCATGC	1 cut(s)	2307
SsiI	CCGC	9 cut(s)	825, 1176, 1959, 2174, 3413, 3627, 3883, 4148, 4512
SspI	AATATT	1 cut(s)	456
SspMI	CTAG	8 cut(s)	68, 1103, 1478, 2007, 2120, 2217, 2397, 3471
SstI	GAGCTC	1 cut(s)	2659
StyD4I	CCNGG	5 cut(s)	226, 234, 1498, 3843, 4197
StyI	CCWWGG	2 cut(s)	2905, 3657
TaaI	ACNGT	8 cut(s)	133, 619, 1334, 1680, 2525, 2781, 3371, 4035
TaiI	ACGT	5 cut(s)	836, 1398, 1772, 3340, 4454
TaqI	TCGA	9 cut(s)	45, 961, 1217, 1521, 1547, 1851, 2323, 3796, 4518
TaqII	GACCGA	1 cut(s)	2049
TatI	WGTACW	7 cut(s)	1212, 3161, 3868, 4027, 4067, 4289, 4357
TauI	GCSGC	2 cut(s)	1178, 3416
TfiI	GAWTC	7 cut(s)	847, 1109, 1784, 3053, 3302, 3820, 3899
TscAI	CASTG	5 cut(s)	157, 2080, 2528, 3449, 3920
TseFI	GTSAC	4 cut(s)	1628, 1660, 3233, 3911
Tsp45I	GTSAC	4 cut(s)	1628, 1660, 3233, 3911
TspGWI	ACGGA	3 cut(s)	453, 1883, 2264
TspRI	CASTG	5 cut(s)	157, 2080, 2528, 3449, 3920
Van91I	CCANNNNNTGG	2 cut(s)	185, 349
Vha464I	CTTAAG	4 cut(s)	1910, 2630, 2945, 4169
VpaK11BI	GGWCC	2 cut(s)	821, 3885
VspI	ATTAAT	3 cut(s)	432, 2879, 4023
XapI	RAATTY	9 cut(s)	998, 1454, 2024, 2531, 2959, 3110, 3138, 3967, 4221
XceI	RCATGY	7 cut(s)	778, 1746, 1765, 2307, 2330, 2389, 3085
XcmI	CCANNNNNNNNNTGG	1 cut(s)	3912
XhoI	CTCGAG	1 cut(s)	1216
XmiI	GTMKAC	1 cut(s)	319
XmnI	GAANNNNTTC	2 cut(s)	934, 1680
XspI	CTAG	8 cut(s)	68, 1103, 1478, 2007, 2120, 2217, 2397, 3471
ZrmI	AGTACT	1 cut(s)	4359

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.