Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr05

Orthogroup ID

OG0016576

Physical Location & Seq

Genomic Coordinates Forward (+)

31630218 .. 31632573

Gene Structure 2356 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD05G1188400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2307 bp

ATGGAACTCGTAACACTCAAAACCCGTCTTTTCATTTCCATTCTCTTCATTGTCTTGGTTTCGGTTTGTGCAAACACTCTCCAGACTTACATTGTTCAGCTCAACCCTCAGGGTGTGACCAGCTCTTCTTTTGCTTCTAAGCCGAGTTGGCATCTCTCGTTTCTTGAACAAACAATGTCTTCCGAAGAAGATGCTTCTTCGCGACTTCTTTACTCTTACCATTCTGCCATGGAAGGGTTTGCCGCTCAGCTATCTGAGTCGGAGCTCGAGTCCTTGAAAATGCTGCCCGATGTCATTGCAATTAGGCCTGACCATAGGCTCCAAATTCACACTACTTATTCTTACAAATTCTTGGGACTCGGCCTCAGTGCTTCGAGGGAAGGTGCTTGGTACAAATCTGCCTTTGGTAGAGGCACAATCATTGGGGTGCTCGATACCGGAGTTTGGCCCGAGAGTCCGAGCTTCAATGATCGTGGAATGCCAGCAGTTCCCAAAAAATGGCGAGGGATTTGCCAAGAAGGGCAGAACTTCAATTCTTCAAATTGCAACCGGAAACTCATTGGTGCTAGGTTTTTCACTAAAGGCCATCGCGTAGCTACGTACACACCAGATGGTGGTCGTGAGTATGTGTCACCGCGTGATTCCCACGGGCATGGAACTCACACATCATCAACAGCAGGGGGCGCTACTGTCCCAATGGCAAGTATTTTCGGCACTGCAGCTGGTGTGGCTCGAGGAATGGCTCCCGGAGCACATATAGCGGTGTACAAAGTGTGCTGGCTCAATGGATGTTATAGCTCTGATATCCTTGCAGCGATGGATGTTGCAATTAGAGATGGAGTAGACATTCTCTCCCTCTCTCTAGGTGGCTTCCCCATTCCACTTTACGATGACAGCATTGCAATTGGCAGTTTTCGAGCAGTGGAGCATGGAATTTCAGTTGTGTGCGCAGCAGGCAACAATGGTCCCATCCAAAGCTCAGTTGCCAATGAAGCTCCTTGGATCACTACCATTGGTGCAAGTACACTAGACCGAAGATTTCCAGGAATAGTTCAAATGGGGAACGGAAAATACCTCTATGGAGAATCTTTGTACCCTGGAAACCACCTTAAGAGAGCAGGGAAAGAGCTTGAACTGGTTTACGTGACAAGTGAGAATAGTGGAAGTGAATATTGCTTCAAAGGGTCTCTTCCACGAGCAAAAGTCCGTGGAAAAATTGTGGTTTGTGACCGAGGTATCAATGGAAGAGCGGAGAAAGGTGAAGTGGTGAAGGAAGCTGGGGGTGCTGCAATGATCTTGGCAAACACAGTGATAAACCAAGAGGAAAACTCAGTTGATGTCCATGTCTTGCCTGCAACTCTTATTGGCTTCAAAGAATCAGTTCACTTGAAAGCTTACATAAACTCTACAAGGAGACCGACAGCTAGAATTGCATTCGGAGGTACAGTTATAGGAAAATCCAGAGCACCAGCAGTAGCTCAGTTCTCTGCTAGAGGACCGAGTTATTCTAACCCTTCAATCCTCAAACCGGATGTGATTGCTCCCGGGGTCAACATAATTGCTGCTTGGCCTCAAAACTTAGGCCCCACCGGCCTTCCAGAAGATTCTAGAAGAGTGAATTTTACTGTCATGTCGGGGACTTCAATGGCCTGTCCACATGGCAGTGGCATCGCGGCTCTGATACGCTCAGCTCATCCCAAATGGAGCCCTGCAGCCATTAAATCTGCCGTTATGACAACTGCTGAAGTAGTTGACCATGCAGGAAAACCGATAATGGATGGAGACAAACCAGCTGGTGTTTTTGCAATTGGTGCCGGACATGTCAACCCTGAAAGAGCCATTGATCCAGGGTTGATTTATGACATCAGGCCAGAGGATTATGTCACGCATTTATGCACTCTTGGATACACAAAATCAGAAATTCTTACCATCACTCACAGGAATGTCAGCTGCCGTGAAGTTTTGAAGATGAACAGGGGTTTCAGCCTCAATTACCCCTCCATTTCAGTAATTTTCAAACATGGTACAAGAAGTAAAATGATCAAAAGACGAGTAACAAATGTGGGGAGTCCTAATTCCATTTACAAGGTGGAAGTAATGGCACCTAAGGAAGTAAAAGTGAGAGTTAAACCCCAAAGGTTAATCTTCACACACATCAATCAGAGTTTGAGTTATAAGGTATGGTTTATATCAAGGAAGAGAACAGAAAAAGGGAAGATGAGCTTTGCACAAGGGCAGCTGACATGGGTGAATTCTAACAATAGCTTCAATCGGGTTAAAAGCCCCTTCTCGGTGACTTGGAAGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004175 GO:0004252 GO:0005575 GO:0005623 GO:0005886 GO:0006508 GO:0006807 GO:0007275 GO:0008150 GO:0008152 GO:0008233 GO:0008236 GO:0009653 GO:0009791 GO:0009888 GO:0009897 GO:0009986 GO:0010016 GO:0010103 GO:0010374 GO:0016020 GO:0016787 GO:0017171 GO:0019538 GO:0032501 GO:0032502 GO:0042127 GO:0043170 GO:0044238 GO:0044425 GO:0044459 GO:0044464 GO:0048367 GO:0048731 GO:0048856 GO:0050789 GO:0050794 GO:0065007 GO:0070011 GO:0071704 GO:0071944 GO:0090558 GO:0090626 GO:0090698 GO:0098552 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Inhibitor_I9 PA Peptidase_S8

Protein Analysis

769

Amino Acids

83.29

Weight (kDa)

9.56

Isoelectric Point (pI)

35.75

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Inhibitor_I9	PF05922	29 - 110	1.2e-19	Peptidase inhibitor I9
Peptidase_S8	PF00082	136 - 585	1.3e-47	Subtilase family
PA	PF02225	377 - 459	6.8e-13	PA domain
fn3_6	PF17766	663 - 765	9.2e-24	Fibronectin type-III domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0016576)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G04110
fragaria_vesca	FvH4_2g20460
malus_domestica	MD05G1188400.v1.1 MD10G1175900.v1.1
prunus_persica	Prupe.8G214900_v2.0.a1
pyrus_communis	pycom10g15150
rosa_chinensis	RchiOBHm_Chr6g0286551
rosa_laevigata	RLG00000012558
rosa_multiflora	Rmu_sc0004628.1_g000013
rosa_roxburghii	Rroxscaffold_7G00182190
rosa_rugosa	Rorug06G0181100
rosa_samantha	Rh6AG293900 Rh6BG297000
rosa_wichuraiana	Rw6G025300

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	2175
Acc16I	TGCGCA	1 cut(s)	949
AccB1I	GGYRCC	2 cut(s)	1811, 2101
AccB7I	CCANNNNNTGG	2 cut(s)	498, 614
AccBSI	CCGCTC	2 cut(s)	245, 1250
AccI	GTMKAC	1 cut(s)	843
AccII	CGCG	4 cut(s)	202, 591, 637, 1673
AciI	CCGC	5 cut(s)	243, 635, 761, 1250, 1673
AclWI	GGATC	2 cut(s)	1010, 1838
AcsI	RAATTY	6 cut(s)	324, 347, 933, 1618, 1920, 2251
AcuI	CTGAAG	1 cut(s)	1764
AdeI	CACNNNGTG	1 cut(s)	638
AfaI	GTAC	7 cut(s)	392, 602, 767, 1024, 1094, 1444, 2026
AfiI	CCNNNNNNNGG	6 cut(s)	234, 444, 498, 614, 1528, 2290
AflII	CTTAAG	1 cut(s)	1109
AflIII	ACRYGT	1 cut(s)	1819
AjnI	CCWGG	3 cut(s)	1042, 1096, 1846
Alw21I	GWGCWC	4 cut(s)	267, 432, 754, 1468
Alw26I	GTCTC	3 cut(s)	1191, 1408, 1776
AlwI	GGATC	2 cut(s)	1010, 1838
Ama87I	CYCGRG	4 cut(s)	266, 449, 732, 1544
AoxI	GGCC	9 cut(s)	305, 361, 446, 583, 1568, 1582, 1591, 1647, 1868
ApeKI	GCWGC	9 cut(s)	283, 719, 812, 950, 1286, 1562, 1712, 1950, 2236
ApoI	RAATTY	6 cut(s)	324, 347, 933, 1618, 1920, 2251
Asp700I	GAANNNNTTC	1 cut(s)	1380
AspLEI	GCGC	2 cut(s)	686, 950
AspS9I	GGNCC	4 cut(s)	447, 965, 1496, 1583
AsuC2I	CCSGG	3 cut(s)	747, 1545, 1546
AsuHPI	GGTGA	5 cut(s)	624, 1271, 1279, 2260, 2305
AvaI	CYCGRG	4 cut(s)	266, 449, 732, 1544
AvaII	GGWCC	2 cut(s)	965, 1496
AxyI	CCTNAGG	2 cut(s)	108, 2106
BaeI	ACNNNNGTAYC	4 cut(s)	426, 459, 1219, 1252
BanI	GGYRCC	2 cut(s)	1811, 2101
BanII	GRGCYC	2 cut(s)	267, 1709
BauI	CACGAG	1 cut(s)	1194
BbsI	GAAGAC	1 cut(s)	171
Bbv12I	GWGCWC	4 cut(s)	267, 432, 754, 1468
BbvI	GCAGC	9 cut(s)	270, 731, 824, 962, 1273, 1549, 1724, 1937, 2248
BccI	CCATC	7 cut(s)	594, 605, 811, 830, 977, 1772, 1937
BceAI	ACGGC	2 cut(s)	1712, 1938
BcgI	CGANNNNNNTGC	8 cut(s)	173, 207, 278, 312, 492, 526, 1651, 1685
BciT130I	CCWGG	3 cut(s)	1044, 1098, 1848
BciVI	GTATCC	1 cut(s)	1898
BclI	TGATCA	1 cut(s)	2040
BcnI	CCSGG	3 cut(s)	747, 1545, 1546
BcoDI	GTCTC	3 cut(s)	1191, 1408, 1776
BfaI	CTAG	6 cut(s)	567, 863, 1028, 1425, 1491, 1608
BfmI	CTRYAG	2 cut(s)	717, 1710
BfoI	RGCGCY	1 cut(s)	687
BfrI	CTTAAG	1 cut(s)	1109
BfuI	GTATCC	1 cut(s)	1898
BglI	GCCNNNNNGGC	2 cut(s)	148, 1590
BlpI	GCTNAGC	2 cut(s)	246, 1687
Bme1390I	CCNGG	6 cut(s)	747, 1044, 1098, 1545, 1546, 1848
Bme18I	GGWCC	2 cut(s)	965, 1496
BmeT110I	CYCGRG	4 cut(s)	266, 449, 732, 1544
BmgT120I	GGNCC	4 cut(s)	447, 965, 1496, 1583
BmiI	GGNNCC	7 cut(s)	320, 744, 967, 1585, 1706, 1813, 2103
BmrFI	CCNGG	6 cut(s)	747, 1044, 1098, 1545, 1546, 1848
BmsI	GCATC	3 cut(s)	160, 181, 1677
BpiI	GAAGAC	1 cut(s)	171
BplI	GAGNNNNNCTC	2 cut(s)	1313, 1345
BpmI	CTGGAG	1 cut(s)	65
Bpu1102I	GCTNAGC	2 cut(s)	246, 1687
BpuMI	CCSGG	3 cut(s)	747, 1545, 1546
BsaAI	YACGTR	2 cut(s)	600, 1144
BsaI	GGTCTC	2 cut(s)	1191, 1408
BsaJI	CCNNGG	9 cut(s)	228, 646, 998, 1096, 1207, 1231, 1544, 1545, 1847
BsaWI	WCCGGW	3 cut(s)	437, 549, 1528
BsaXI	ACNNNNNCTCC	6 cut(s)	254, 284, 836, 866, 1431, 1461
Bsc4I	CCNNNNNNNGG	6 cut(s)	234, 444, 498, 614, 1528, 2290
Bse118I	RCCGGY	1 cut(s)	1589
Bse1I	ACTGG	1 cut(s)	1140
Bse21I	CCTNAGG	2 cut(s)	108, 2106
Bse3DI	GCAATG	3 cut(s)	294, 897, 1296
BseBI	CCWGG	3 cut(s)	1044, 1098, 1848
BseDI	CCNNGG	9 cut(s)	228, 646, 998, 1096, 1207, 1231, 1544, 1545, 1847
BseGI	GGATG	6 cut(s)	794, 826, 969, 1537, 1693, 1783
BseLI	CCNNNNNNNGG	6 cut(s)	234, 444, 498, 614, 1528, 2290
BseMI	GCAATG	3 cut(s)	294, 897, 1296
BseMII	CTCAG	8 cut(s)	122, 246, 260, 379, 993, 1344, 1493, 1701
BseNI	ACTGG	1 cut(s)	1140
BseXI	GCAGC	9 cut(s)	270, 731, 824, 962, 1273, 1549, 1724, 1937, 2248
BseYI	CCCAGC	1 cut(s)	1277
Bsh1236I	CGCG	4 cut(s)	202, 591, 637, 1673
BshFI	GGCC	9 cut(s)	307, 363, 448, 585, 1570, 1584, 1593, 1649, 1870
BshNI	GGYRCC	2 cut(s)	1811, 2101
BsiHKAI	GWGCWC	4 cut(s)	267, 432, 754, 1468
BsiHKCI	CYCGRG	4 cut(s)	266, 449, 732, 1544
BsiSI	CCGG	7 cut(s)	438, 550, 747, 1529, 1545, 1590, 1815
BslFI	GGGAC	4 cut(s)	369, 677, 951, 1651
BslI	CCNNNNNNNGG	6 cut(s)	234, 444, 498, 614, 1528, 2290
BsmAI	GTCTC	3 cut(s)	1191, 1408, 1776
BsmFI	GGGAC	4 cut(s)	369, 677, 951, 1651
BsmI	GAATGC	2 cut(s)	483, 1433
BsnI	GGCC	9 cut(s)	307, 363, 448, 585, 1570, 1584, 1593, 1649, 1870
Bso31I	GGTCTC	2 cut(s)	1191, 1408
BsoBI	CYCGRG	4 cut(s)	266, 449, 732, 1544
Bsp1286I	GDGCHC	5 cut(s)	267, 432, 754, 1468, 1709
Bsp1407I	TGTACA	1 cut(s)	765
Bsp143I	GATC	5 cut(s)	469, 1002, 1293, 1843, 2040
Bsp1720I	GCTNAGC	2 cut(s)	246, 1687
Bsp19I	CCATGG	1 cut(s)	228
Bsp68I	TCGCGA	1 cut(s)	202
BspACI	CCGC	5 cut(s)	243, 635, 761, 1250, 1673
BspANI	GGCC	9 cut(s)	307, 363, 448, 585, 1570, 1584, 1593, 1649, 1870
BspCNI	CTCAG	8 cut(s)	121, 247, 259, 378, 992, 1343, 1492, 1700
BspFNI	CGCG	4 cut(s)	202, 591, 637, 1673
BspLI	GGNNCC	7 cut(s)	320, 744, 967, 1585, 1706, 1813, 2103
BspMAI	CTGCAG	2 cut(s)	721, 1714
BspPI	GGATC	2 cut(s)	1010, 1838
BspQI	GCTCTTC	2 cut(s)	130, 1240
BspT107I	GGYRCC	2 cut(s)	1811, 2101
BspTI	CTTAAG	1 cut(s)	1109
BspTNI	GGTCTC	2 cut(s)	1191, 1408
BsrBI	CCGCTC	2 cut(s)	245, 1250
BsrDI	GCAATG	3 cut(s)	294, 897, 1296
BsrFI	RCCGGY	1 cut(s)	1589
BsrGI	TGTACA	1 cut(s)	765
BsrI	ACTGG	1 cut(s)	1140
BssAI	RCCGGY	1 cut(s)	1589
BssECI	CCNNGG	9 cut(s)	228, 646, 998, 1096, 1207, 1231, 1544, 1545, 1847
BssMI	GATC	5 cut(s)	469, 1002, 1293, 1843, 2040
BssSI	CACGAG	1 cut(s)	1194
BssT1I	CCWWGG	2 cut(s)	228, 998
Bst2BI	CACGAG	1 cut(s)	1194
Bst2UI	CCWGG	3 cut(s)	1044, 1098, 1848
Bst4CI	ACNGT	4 cut(s)	691, 1309, 1447, 1627
Bst6I	CTCTTC	6 cut(s)	50, 130, 1194, 1240, 1606, 2192
BstAFI	CTTAAG	1 cut(s)	1109
BstAPI	GCANNNNNTGC	1 cut(s)	1811
BstAUI	TGTACA	1 cut(s)	765
BstBAI	YACGTR	2 cut(s)	600, 1144
BstC8I	GCNNGC	4 cut(s)	483, 779, 955, 1353
BstDSI	CCRYGG	3 cut(s)	228, 646, 1207
BstF5I	GGATG	6 cut(s)	794, 826, 969, 1537, 1693, 1783
BstFNI	CGCG	4 cut(s)	202, 591, 637, 1673
BstH2I	RGCGCY	1 cut(s)	687
BstHHI	GCGC	2 cut(s)	686, 950
BstKTI	GATC	5 cut(s)	472, 1005, 1296, 1846, 2043
BstMAI	GTCTC	3 cut(s)	1191, 1408, 1776
BstMBI	GATC	5 cut(s)	469, 1002, 1293, 1843, 2040
BstNI	CCWGG	3 cut(s)	1044, 1098, 1848
BstNSI	RCATGY	1 cut(s)	1823
BstSCI	CCNGG	6 cut(s)	745, 1042, 1096, 1543, 1544, 1846
BstSFI	CTRYAG	2 cut(s)	717, 1710
BstSNI	TACGTA	1 cut(s)	600
BstUI	CGCG	4 cut(s)	202, 591, 637, 1673
BstV1I	GCAGC	9 cut(s)	270, 731, 824, 962, 1273, 1549, 1724, 1937, 2248
BstV2I	GAAGAC	1 cut(s)	171
BstXI	CCANNNNNNTGG	1 cut(s)	653
Bsu36I	CCTNAGG	2 cut(s)	108, 2106
BsuI	GTATCC	1 cut(s)	1898
BsuRI	GGCC	9 cut(s)	307, 363, 448, 585, 1570, 1584, 1593, 1649, 1870
BtgI	CCRYGG	3 cut(s)	228, 646, 1207
BtgZI	GCGATG	3 cut(s)	572, 830, 1654
BtsCI	GGATG	6 cut(s)	794, 826, 969, 1537, 1693, 1783
BtsI	GCAGTG	3 cut(s)	714, 927, 1669
BtsIMutI	CAGTG	5 cut(s)	373, 714, 927, 1314, 1669
BtuMI	TCGCGA	1 cut(s)	202
Cac8I	GCNNGC	4 cut(s)	483, 779, 955, 1353
CfoI	GCGC	2 cut(s)	686, 950
Cfr10I	RCCGGY	1 cut(s)	1589
Cfr13I	GGNCC	4 cut(s)	447, 965, 1496, 1583
Cfr9I	CCCGGG	1 cut(s)	1544
Csp6I	GTAC	7 cut(s)	391, 601, 766, 1023, 1093, 1443, 2025
CspCI	CAANNNNNGTGG	2 cut(s)	454, 489
CviQI	GTAC	7 cut(s)	391, 601, 766, 1023, 1093, 1443, 2025
DpnI	GATC	5 cut(s)	471, 1004, 1295, 1845, 2042
DpnII	GATC	5 cut(s)	469, 1002, 1293, 1843, 2040
DraIII	CACNNNGTG	1 cut(s)	638
Eam1104I	CTCTTC	6 cut(s)	50, 130, 1194, 1240, 1606, 2192
EarI	CTCTTC	6 cut(s)	50, 130, 1194, 1240, 1606, 2192
Ecl136II	GAGCTC	1 cut(s)	265
Eco105I	TACGTA	1 cut(s)	600
Eco130I	CCWWGG	2 cut(s)	228, 998
Eco147I	AGGCCT	1 cut(s)	307
Eco24I	GRGCYC	2 cut(s)	267, 1709
Eco31I	GGTCTC	2 cut(s)	1191, 1408
Eco32I	GATATC	1 cut(s)	805
Eco47I	GGWCC	2 cut(s)	965, 1496
Eco53kI	GAGCTC	1 cut(s)	265
Eco57I	CTGAAG	1 cut(s)	1764
Eco81I	CCTNAGG	2 cut(s)	108, 2106
Eco88I	CYCGRG	4 cut(s)	266, 449, 732, 1544
EcoICRI	GAGCTC	1 cut(s)	265
EcoO109I	RGGNCCY	1 cut(s)	1583
EcoRI	GAATTC	1 cut(s)	2251
EcoRII	CCWGG	3 cut(s)	1042, 1096, 1846
EcoRV	GATATC	1 cut(s)	805
EcoT14I	CCWWGG	2 cut(s)	228, 998
EcoT38I	GRGCYC	2 cut(s)	267, 1709
ErhI	CCWWGG	2 cut(s)	228, 998
FalI	AAGNNNNNCTT	4 cut(s)	1173, 1205, 2207, 2239
FaqI	GGGAC	4 cut(s)	369, 677, 951, 1651
FbaI	TGATCA	1 cut(s)	2040
FblI	GTMKAC	1 cut(s)	843
FokI	GGATG	6 cut(s)	801, 833, 956, 1544, 1680, 1790
FriOI	GRGCYC	2 cut(s)	267, 1709
FspBI	CTAG	6 cut(s)	567, 863, 1028, 1425, 1491, 1608
FspI	TGCGCA	1 cut(s)	949
GlaI	GCGC	2 cut(s)	685, 949
GsaI	CCCAGC	1 cut(s)	1281
GsuI	CTGGAG	1 cut(s)	65
HaeII	RGCGCY	1 cut(s)	687
HaeIII	GGCC	9 cut(s)	307, 363, 448, 585, 1570, 1584, 1593, 1649, 1870
HapII	CCGG	7 cut(s)	438, 550, 747, 1529, 1545, 1590, 1815
HhaI	GCGC	2 cut(s)	686, 950
Hin6I	GCGC	2 cut(s)	684, 948
HinP1I	GCGC	2 cut(s)	684, 948
HincII	GTYRAC	3 cut(s)	1552, 1753, 1825
HindII	GTYRAC	3 cut(s)	1552, 1753, 1825
HindIII	AAGCTT	1 cut(s)	1392
HinfI	GANTC	9 cut(s)	257, 269, 357, 454, 641, 1085, 1376, 1604, 2068
HpaII	CCGG	7 cut(s)	438, 550, 747, 1529, 1545, 1590, 1815
HphI	GGTGA	5 cut(s)	624, 1271, 1279, 2260, 2305
Hpy188I	TCNGA	9 cut(s)	184, 256, 262, 459, 802, 1439, 1680, 1918, 2163
Hpy188III	TCNNGA	7 cut(s)	82, 164, 201, 620, 1461, 1598, 1608
HpyAV	CCTTC	7 cut(s)	227, 374, 512, 1264, 1524, 1604, 2296
HpyCH4III	ACNGT	4 cut(s)	691, 1309, 1447, 1627
HpyCH4IV	ACGT	2 cut(s)	599, 1143
HpySE526I	ACGT	2 cut(s)	599, 1143
HspAI	GCGC	2 cut(s)	684, 948
Ksp22I	TGATCA	1 cut(s)	2040
Kzo9I	GATC	5 cut(s)	469, 1002, 1293, 1843, 2040
LguI	GCTCTTC	2 cut(s)	130, 1240
LmnI	GCTCC	8 cut(s)	262, 324, 748, 749, 925, 1000, 1546, 1704
Lsp1109I	GCAGC	9 cut(s)	270, 731, 824, 962, 1273, 1549, 1724, 1937, 2248
LweI	GCATC	3 cut(s)	160, 181, 1677
MaeI	CTAG	6 cut(s)	567, 863, 1028, 1425, 1491, 1608
MaeII	ACGT	2 cut(s)	599, 1143
MaeIII	GTNAC	8 cut(s)	10, 115, 630, 1144, 1226, 1882, 2053, 2293
MalI	GATC	5 cut(s)	471, 1004, 1295, 1845, 2042
MbiI	CCGCTC	2 cut(s)	245, 1250
MboI	GATC	5 cut(s)	469, 1002, 1293, 1843, 2040
MfeI	CAATTG	2 cut(s)	903, 1806
MhlI	GDGCHC	5 cut(s)	267, 432, 754, 1468, 1709
MlyI	GAGTC	5 cut(s)	266, 278, 351, 463, 2077
MmeI	TCCRAC	1 cut(s)	240
MroXI	GAANNNNTTC	1 cut(s)	1380
MseI	TTAA	5 cut(s)	1110, 1719, 2127, 2141, 2277
MslI	CAYNNNNRTG	4 cut(s)	425, 651, 1662, 1941
MspA1I	CMGCKG	4 cut(s)	722, 1793, 1950, 2239
MspCI	CTTAAG	1 cut(s)	1109
MspI	CCGG	7 cut(s)	438, 550, 747, 1529, 1545, 1590, 1815
MspR9I	CCNGG	6 cut(s)	747, 1044, 1098, 1545, 1546, 1848
MunI	CAATTG	2 cut(s)	903, 1806
Mva1269I	GAATGC	2 cut(s)	483, 1433
MvaI	CCWGG	3 cut(s)	1044, 1098, 1848
MvnI	CGCG	4 cut(s)	202, 591, 637, 1673
NciI	CCSGG	3 cut(s)	747, 1545, 1546
NcoI	CCATGG	1 cut(s)	228
NdeII	GATC	5 cut(s)	469, 1002, 1293, 1843, 2040
NlaIV	GGNNCC	7 cut(s)	320, 744, 967, 1585, 1706, 1813, 2103
NmeAIII	GCCGAG	2 cut(s)	168, 339
NmuCI	GTSAC	6 cut(s)	115, 630, 1144, 1226, 1882, 2293
NruI	TCGCGA	1 cut(s)	202
NsbI	TGCGCA	1 cut(s)	949
NspI	RCATGY	1 cut(s)	1823
PaeR7I	CTCGAG	2 cut(s)	266, 732
PceI	AGGCCT	1 cut(s)	307
PciI	ACATGT	1 cut(s)	1819
PciSI	GCTCTTC	2 cut(s)	130, 1240
PctI	GAATGC	2 cut(s)	483, 1433
PdmI	GAANNNNTTC	1 cut(s)	1380
PfeI	GAWTC	4 cut(s)	641, 1085, 1376, 1604
PflMI	CCANNNNNTGG	2 cut(s)	498, 614
PfoI	TCCNGGA	2 cut(s)	745, 1042
PleI	GAGTC	5 cut(s)	265, 277, 351, 462, 2076
PpsI	GAGTC	5 cut(s)	265, 277, 351, 462, 2076
Ppu21I	YACGTR	2 cut(s)	600, 1144
PscI	ACATGT	1 cut(s)	1819
PsiI	TTATAA	1 cut(s)	2175
Psp124BI	GAGCTC	1 cut(s)	267
Psp6I	CCWGG	3 cut(s)	1042, 1096, 1846
PspFI	CCCAGC	1 cut(s)	1277
PspGI	CCWGG	3 cut(s)	1042, 1096, 1846
PspN4I	GGNNCC	7 cut(s)	320, 744, 967, 1585, 1706, 1813, 2103
PspPI	GGNCC	4 cut(s)	447, 965, 1496, 1583
PspXI	VCTCGAGB	2 cut(s)	266, 732
PsrI	GAACNNNNNNTAC	2 cut(s)	1055, 1087
PstI	CTGCAG	2 cut(s)	721, 1714
PvuII	CAGCTG	4 cut(s)	722, 1793, 1950, 2239
RruI	TCGCGA	1 cut(s)	202
RsaI	GTAC	7 cut(s)	392, 602, 767, 1024, 1094, 1444, 2026
RsaNI	GTAC	7 cut(s)	391, 601, 766, 1023, 1093, 1443, 2025
RseI	CAYNNNNRTG	4 cut(s)	425, 651, 1662, 1941
SacI	GAGCTC	1 cut(s)	267
SapI	GCTCTTC	2 cut(s)	130, 1240
SaqAI	TTAA	5 cut(s)	1110, 1719, 2127, 2141, 2277
Sau3AI	GATC	5 cut(s)	469, 1002, 1293, 1843, 2040
Sau96I	GGNCC	4 cut(s)	447, 965, 1496, 1583
SchI	GAGTC	5 cut(s)	266, 278, 351, 463, 2077
ScrFI	CCNGG	6 cut(s)	747, 1044, 1098, 1545, 1546, 1848
SduI	GDGCHC	5 cut(s)	267, 432, 754, 1468, 1709
SfaNI	GCATC	3 cut(s)	160, 181, 1677
SfcI	CTRYAG	2 cut(s)	717, 1710
SfiI	GGCCNNNNNGGCC	1 cut(s)	1590
Sfr274I	CTCGAG	2 cut(s)	266, 732
SinI	GGWCC	2 cut(s)	965, 1496
SlaI	CTCGAG	2 cut(s)	266, 732
SmaI	CCCGGG	1 cut(s)	1546
SmiMI	CAYNNNNRTG	4 cut(s)	425, 651, 1662, 1941
SmlI	CTYRAG	3 cut(s)	266, 732, 1109
SmoI	CTYRAG	3 cut(s)	266, 732, 1109
SnaBI	TACGTA	1 cut(s)	600
SseBI	AGGCCT	1 cut(s)	307
SsiI	CCGC	5 cut(s)	243, 635, 761, 1250, 1673
SspI	AATATT	1 cut(s)	1172
SspMI	CTAG	6 cut(s)	567, 863, 1028, 1425, 1491, 1608
SstI	GAGCTC	1 cut(s)	267
StuI	AGGCCT	1 cut(s)	307
StyD4I	CCNGG	6 cut(s)	745, 1042, 1096, 1543, 1544, 1846
StyI	CCWWGG	2 cut(s)	228, 998
TaaI	ACNGT	4 cut(s)	691, 1309, 1447, 1627
TaiI	ACGT	2 cut(s)	602, 1146
TaqI	TCGA	5 cut(s)	267, 374, 432, 733, 916
TaqII	GACCGA	4 cut(s)	1047, 1245, 1432, 1513
TatI	WGTACW	2 cut(s)	765, 1022
TauI	GCSGC	2 cut(s)	245, 1676
TfiI	GAWTC	4 cut(s)	641, 1085, 1376, 1604
Tru1I	TTAA	5 cut(s)	1110, 1719, 2127, 2141, 2277
Tru9I	TTAA	5 cut(s)	1110, 1719, 2127, 2141, 2277
TscAI	CASTG	5 cut(s)	373, 721, 927, 1314, 1669
TseFI	GTSAC	6 cut(s)	115, 630, 1144, 1226, 1882, 2293
TseI	GCWGC	9 cut(s)	283, 719, 812, 950, 1286, 1562, 1712, 1950, 2236
Tsp45I	GTSAC	6 cut(s)	115, 630, 1144, 1226, 1882, 2293
TspDTI	ATGAA	4 cut(s)	22, 37, 1005, 1985
TspGWI	ACGGA	2 cut(s)	1080, 1196
TspMI	CCCGGG	1 cut(s)	1544
TspRI	CASTG	5 cut(s)	373, 721, 927, 1314, 1669
Van91I	CCANNNNNTGG	2 cut(s)	498, 614
Vha464I	CTTAAG	1 cut(s)	1109
VpaK11BI	GGWCC	2 cut(s)	965, 1496
XapI	RAATTY	6 cut(s)	324, 347, 933, 1618, 1920, 2251
XbaI	TCTAGA	1 cut(s)	1607
XceI	RCATGY	1 cut(s)	1823
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2086
XhoI	CTCGAG	2 cut(s)	266, 732
XmaI	CCCGGG	1 cut(s)	1544
XmiI	GTMKAC	1 cut(s)	843
XmnI	GAANNNNTTC	1 cut(s)	1380
XspI	CTAG	6 cut(s)	567, 863, 1028, 1425, 1491, 1608

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.