Rorug06G0181100

Photosystem II reaction center PSB28 protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0010122
Physical Location & Seq
Reverse (-)
28623320 .. 28628641
5322 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0181100.1

Sequence Viewer

Length: 435 bp
ATGTTCTCTGGTCATGAAGGTTTCTGTAGCTTGAAAATAAGTTATAAAGAGGGCTATCTTTTGGGTTTTGCAGAGGAGACTTCGAAATTCGAATTGTGTTCTCTGGTCATGAAGGTTTCCGTGTTTCAGACAAAAGATGTTGGCTACATAGGAAACCAACAGCAGAACATAGCCGATGCTATCGATCTACTCCCTCGACTTGCAACCTATTTCGTTGTTAACATAAGGAAAAGTGATTTTGAGTTTACTCCGGAATGTACAATATTTGATATGTTAGACATCCCACTATATCATGGCTGGATAGTTGATCCCCAGGATAGCTTCCTTGTAGAGGAGGTGTTTGAACAATTGAAGTGTACAACAGAGGGTCTCAAATCTGAGGAGGTGCAAAAGCATCTGGAGCATGTTGTCAAATGTGGATTAGATCGATTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00195 ko01100 map00195 map01100
Pfam Domains
Protein Families
Psb28

Protein Analysis

144

Amino Acids

16.55

Weight (kDa)

4.75

Isoelectric Point (pI)

34.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MINDY_DUB PF04424 27 - 113 2.1e-17 MINDY deubiquitinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 45
AccIII TCCGGA 1 cut(s) 250
AclWI GGATC 1 cut(s) 302
AcsI RAATTY 1 cut(s) 86
AfaI GTAC 2 cut(s) 259, 358
AfiI CCNNNNNNNGG 1 cut(s) 331
AgsI TTSAA 3 cut(s) 34, 344, 352
AjnI CCWGG 1 cut(s) 312
AluBI AGCT 2 cut(s) 30, 321
AluI AGCT 2 cut(s) 30, 321
Alw26I GTCTC 2 cut(s) 71, 374
AlwI GGATC 1 cut(s) 302
Aor13HI TCCGGA 1 cut(s) 250
ApoI RAATTY 1 cut(s) 86
AsuII TTCGAA 2 cut(s) 83, 90
BciT130I CCWGG 1 cut(s) 314
BcoDI GTCTC 2 cut(s) 71, 374
BfmI CTRYAG 1 cut(s) 25
Bme1390I CCNGG 1 cut(s) 314
BmrFI CCNGG 1 cut(s) 314
BmsI GCATC 2 cut(s) 166, 403
BpmI CTGGAG 1 cut(s) 419
Bpu14I TTCGAA 2 cut(s) 83, 90
Bsa29I ATCGAT 2 cut(s) 183, 427
BsaI GGTCTC 1 cut(s) 374
BsaJI CCNNGG 1 cut(s) 312
BsaWI WCCGGW 1 cut(s) 250
Bsc4I CCNNNNNNNGG 1 cut(s) 331
BseAI TCCGGA 1 cut(s) 250
BseBI CCWGG 1 cut(s) 314
BseCI ATCGAT 2 cut(s) 183, 427
BseDI CCNNGG 1 cut(s) 312
BseGI GGATG 1 cut(s) 279
BseLI CCNNNNNNNGG 1 cut(s) 331
BseMII CTCAG 1 cut(s) 369
BseRI GAGGAG 3 cut(s) 89, 347, 395
BshVI ATCGAT 2 cut(s) 183, 427
BsiSI CCGG 1 cut(s) 251
BslI CCNNNNNNNGG 1 cut(s) 331
BsmAI GTCTC 2 cut(s) 71, 374
Bso31I GGTCTC 1 cut(s) 374
Bsp119I TTCGAA 2 cut(s) 83, 90
Bsp13I TCCGGA 1 cut(s) 250
Bsp1407I TGTACA 2 cut(s) 257, 356
Bsp143I GATC 3 cut(s) 184, 307, 424
BspCNI CTCAG 1 cut(s) 370
BspDI ATCGAT 2 cut(s) 183, 427
BspEI TCCGGA 1 cut(s) 250
BspHI TCATGA 2 cut(s) 13, 108
BspPI GGATC 1 cut(s) 302
BspT104I TTCGAA 2 cut(s) 83, 90
BspTNI GGTCTC 1 cut(s) 374
BsrGI TGTACA 2 cut(s) 257, 356
BssECI CCNNGG 1 cut(s) 312
BssMI GATC 3 cut(s) 184, 307, 424
Bst2UI CCWGG 1 cut(s) 314
BstAUI TGTACA 2 cut(s) 257, 356
BstBI TTCGAA 2 cut(s) 83, 90
BstDEI CTNAG 1 cut(s) 378
BstENI CCTNNNNNAGG 1 cut(s) 329
BstF5I GGATG 1 cut(s) 279
BstKTI GATC 3 cut(s) 187, 310, 427
BstMAI GTCTC 2 cut(s) 71, 374
BstMBI GATC 3 cut(s) 184, 307, 424
BstMWI GCNNNNNNNGC 1 cut(s) 400
BstNI CCWGG 1 cut(s) 314
BstNSI RCATGY 1 cut(s) 407
BstSCI CCNGG 1 cut(s) 312
BstSFI CTRYAG 1 cut(s) 25
Bsu15I ATCGAT 2 cut(s) 183, 427
BsuTUI ATCGAT 2 cut(s) 183, 427
BtsCI GGATG 1 cut(s) 279
CciI TCATGA 2 cut(s) 13, 108
ClaI ATCGAT 2 cut(s) 183, 427
Csp6I GTAC 2 cut(s) 258, 357
CviAII CATG 4 cut(s) 14, 109, 293, 404
CviJI RGCY 6 cut(s) 30, 54, 144, 173, 297, 321
CviKI_1 RGCY 6 cut(s) 30, 54, 144, 173, 297, 321
CviQI GTAC 2 cut(s) 258, 357
DdeI CTNAG 1 cut(s) 378
DpnI GATC 3 cut(s) 186, 309, 426
DpnII GATC 3 cut(s) 184, 307, 424
Eco31I GGTCTC 1 cut(s) 374
EcoNI CCTNNNNNAGG 1 cut(s) 329
EcoRII CCWGG 1 cut(s) 312
FaeI CATG 4 cut(s) 17, 112, 296, 407
FatI CATG 4 cut(s) 13, 108, 292, 403
FokI GGATG 1 cut(s) 266
GsuI CTGGAG 1 cut(s) 419
HapII CCGG 1 cut(s) 251
Hin1II CATG 4 cut(s) 17, 112, 296, 407
HincII GTYRAC 1 cut(s) 220
HindII GTYRAC 1 cut(s) 220
HpaI GTTAAC 1 cut(s) 220
HpaII CCGG 1 cut(s) 251
Hpy166II GTNNAC 3 cut(s) 220, 246, 357
Hpy188I TCNGA 2 cut(s) 129, 379
Hpy188III TCNNGA 4 cut(s) 14, 109, 251, 398
Hpy8I GTNNAC 3 cut(s) 220, 246, 357
HpyAV CCTTC 2 cut(s) 11, 106
HpyCH4V TGCA 3 cut(s) 71, 203, 388
HpyF10VI GCNNNNNNNGC 1 cut(s) 400
HpyF3I CTNAG 1 cut(s) 378
Hsp92II CATG 4 cut(s) 17, 112, 296, 407
Kpn2I TCCGGA 1 cut(s) 250
KspAI GTTAAC 1 cut(s) 220
Kzo9I GATC 3 cut(s) 184, 307, 424
LmnI GCTCC 1 cut(s) 400
LpnPI CCDG 6 cut(s) 89, 264, 283, 299, 326, 383
LweI GCATC 2 cut(s) 166, 403
MalI GATC 3 cut(s) 186, 309, 426
MboI GATC 3 cut(s) 184, 307, 424
MfeI CAATTG 1 cut(s) 347
MluCI AATT 3 cut(s) 86, 92, 347
MnlI CCTC 8 cut(s) 43, 67, 204, 325, 328, 358, 373, 376
MroI TCCGGA 1 cut(s) 250
MseI TTAA 1 cut(s) 219
MspI CCGG 1 cut(s) 251
MspR9I CCNGG 1 cut(s) 314
MunI CAATTG 1 cut(s) 347
MvaI CCWGG 1 cut(s) 314
MwoI GCNNNNNNNGC 1 cut(s) 400
NdeII GATC 3 cut(s) 184, 307, 424
NlaIII CATG 4 cut(s) 17, 112, 296, 407
NspI RCATGY 1 cut(s) 407
NspV TTCGAA 2 cut(s) 83, 90
PagI TCATGA 2 cut(s) 13, 108
PsiI TTATAA 1 cut(s) 45
Psp6I CCWGG 1 cut(s) 312
PspGI CCWGG 1 cut(s) 312
RsaI GTAC 2 cut(s) 259, 358
RsaNI GTAC 2 cut(s) 258, 357
SaqAI TTAA 1 cut(s) 219
Sau3AI GATC 3 cut(s) 184, 307, 424
ScrFI CCNGG 1 cut(s) 314
SetI ASST 7 cut(s) 22, 32, 117, 209, 323, 339, 387
SfaNI GCATC 2 cut(s) 166, 403
SfcI CTRYAG 1 cut(s) 25
SfuI TTCGAA 2 cut(s) 83, 90
Sse9I AATT 3 cut(s) 86, 92, 347
SspI AATATT 1 cut(s) 264
StyD4I CCNGG 1 cut(s) 312
TaqI TCGA 5 cut(s) 83, 90, 183, 196, 427
TasI AATT 3 cut(s) 86, 92, 347
TatI WGTACW 2 cut(s) 257, 356
Tru1I TTAA 1 cut(s) 219
Tru9I TTAA 1 cut(s) 219
TspDTI ATGAA 2 cut(s) 30, 125
TspGWI ACGGA 1 cut(s) 109
XagI CCTNNNNNAGG 1 cut(s) 329
XapI RAATTY 1 cut(s) 86
XceI RCATGY 1 cut(s) 407
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.