Rorug06G0181000

Photosystem II reaction center PSB28 protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0010122
Physical Location & Seq
Reverse (-)
28619729 .. 28620992
1264 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0181000.1

Sequence Viewer

Length: 522 bp
ATGGCTGAGCCCGATGACTGGATACCTCGATTGGAAAATGGAGGCGAAGAAATTCCAAACTACGTTCACCCTGATTATTTTACCACCAAGGTATACCATGGTGGCTATATAGATCACATTGAAGGTAAGTACATTGGAGGAAGCATCAGTTACTACGATAACGTTGACAAAGACAGAATGTCGTTGACTGAAGTGGATGGCATGGTGAGGGGCATCAACCCTAAATACGCTGGGACAAGGATAGATTACTGGTATTTTATTGGCAGTGAGGAGGATGGAAAGACAAAGCTGAGCTGTGATAGTGATGTGATCAGAATGTGTTGCCTGGTGCCTGAAATAAGGTTGGTGATACTTTACCTTCACCATTTGGAGCTGGACCCTGCTTATGAAGAATCTTTGGATGATTTGTTTATGTATGAAGACCAAATACCTGGTTGGAGTCAAGCTGCGTCAAGCAATGTTGTCATAGAAGAGCTACCAGATTCACCAACAAAGCCAAGAACATGCAAGTGTCAAGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00195 ko01100 map00195 map01100
Pfam Domains
Protein Families
Psb28

Protein Analysis

173

Amino Acids

19.84

Weight (kDa)

4.45

Isoelectric Point (pI)

49.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PB1-like PF26130 25 - 110 2.9e-11 PB1-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 328
AccI GTMKAC 1 cut(s) 93
AclI AACGTT 1 cut(s) 162
AcsI RAATTY 1 cut(s) 51
AcuI CTGAAG 1 cut(s) 210
AfaI GTAC 1 cut(s) 131
AfiI CCNNNNNNNGG 1 cut(s) 18
AgsI TTSAA 1 cut(s) 122
AhdI GACNNNNNGTC 1 cut(s) 178
AjnI CCWGG 2 cut(s) 324, 430
AluBI AGCT 5 cut(s) 289, 294, 373, 446, 475
AluI AGCT 5 cut(s) 289, 294, 373, 446, 475
ApeKI GCWGC 1 cut(s) 446
ApoI RAATTY 1 cut(s) 51
Asp700I GAANNNNTTC 1 cut(s) 51
AspS9I GGNCC 1 cut(s) 376
AsuHPI GGTGA 5 cut(s) 59, 217, 353, 358, 477
AvaII GGWCC 1 cut(s) 376
BanI GGYRCC 1 cut(s) 328
BanII GRGCYC 1 cut(s) 12
BarI GAAGNNNNNNTAC 2 cut(s) 411, 443
BbsI GAAGAC 1 cut(s) 426
BbvI GCAGC 1 cut(s) 433
BccI CCATC 2 cut(s) 191, 269
BciT130I CCWGG 2 cut(s) 326, 432
BciVI GTATCC 1 cut(s) 15
BclI TGATCA 1 cut(s) 309
BfuI GTATCC 1 cut(s) 15
BisI GCNGC 1 cut(s) 447
BlpI GCTNAGC 2 cut(s) 6, 290
BlsI GCNGC 1 cut(s) 448
Bme1390I CCNGG 2 cut(s) 326, 432
Bme18I GGWCC 1 cut(s) 376
BmeRI GACNNNNNGTC 1 cut(s) 178
BmgT120I GGNCC 1 cut(s) 376
BmiI GGNNCC 2 cut(s) 330, 378
BmrFI CCNGG 2 cut(s) 326, 432
BmsI GCATC 2 cut(s) 153, 222
BpiI GAAGAC 1 cut(s) 426
Bpu1102I GCTNAGC 2 cut(s) 6, 290
BsaJI CCNNGG 2 cut(s) 87, 97
Bsc4I CCNNNNNNNGG 1 cut(s) 18
Bse1I ACTGG 2 cut(s) 23, 254
Bse3DI GCAATG 1 cut(s) 463
BseBI CCWGG 2 cut(s) 326, 432
BseDI CCNNGG 2 cut(s) 87, 97
BseGI GGATG 3 cut(s) 202, 280, 406
BseLI CCNNNNNNNGG 1 cut(s) 18
BseMI GCAATG 1 cut(s) 463
BseMII CTCAG 1 cut(s) 281
BseNI ACTGG 2 cut(s) 23, 254
BseRI GAGGAG 1 cut(s) 284
BseXI GCAGC 1 cut(s) 433
BseYI CCCAGC 1 cut(s) 230
BshNI GGYRCC 1 cut(s) 328
BslFI GGGAC 1 cut(s) 247
BslI CCNNNNNNNGG 1 cut(s) 18
BsmFI GGGAC 1 cut(s) 247
Bsp1286I GDGCHC 1 cut(s) 12
Bsp143I GATC 2 cut(s) 112, 309
Bsp1720I GCTNAGC 2 cut(s) 6, 290
Bsp19I CCATGG 1 cut(s) 97
BspCNI CTCAG 1 cut(s) 282
BspLI GGNNCC 2 cut(s) 330, 378
BspQI GCTCTTC 1 cut(s) 465
BspT107I GGYRCC 1 cut(s) 328
BsrDI GCAATG 1 cut(s) 463
BsrI ACTGG 2 cut(s) 23, 254
BssECI CCNNGG 2 cut(s) 87, 97
BssMI GATC 2 cut(s) 112, 309
BssNAI GTATAC 1 cut(s) 94
BssT1I CCWWGG 2 cut(s) 87, 97
Bst1107I GTATAC 1 cut(s) 94
Bst2UI CCWGG 2 cut(s) 326, 432
Bst6I CTCTTC 1 cut(s) 465
BstDEI CTNAG 2 cut(s) 6, 290
BstDSI CCRYGG 1 cut(s) 97
BstF5I GGATG 3 cut(s) 202, 280, 406
BstKTI GATC 2 cut(s) 115, 312
BstMBI GATC 2 cut(s) 112, 309
BstNI CCWGG 2 cut(s) 326, 432
BstNSI RCATGY 1 cut(s) 507
BstSCI CCNGG 2 cut(s) 324, 430
BstV1I GCAGC 1 cut(s) 433
BstV2I GAAGAC 1 cut(s) 426
BstXI CCANNNNNNTGG 1 cut(s) 431
BstZ17I GTATAC 1 cut(s) 94
BsuI GTATCC 1 cut(s) 15
BtgI CCRYGG 1 cut(s) 97
BtsCI GGATG 3 cut(s) 202, 280, 406
BtsI GCAGTG 1 cut(s) 271
BtsIMutI CAGTG 1 cut(s) 271
Cfr13I GGNCC 1 cut(s) 376
CseI GACGC 1 cut(s) 438
CsiI ACCWGGT 1 cut(s) 430
Csp6I GTAC 1 cut(s) 130
CviAII CATG 3 cut(s) 98, 202, 504
CviJI RGCY 9 cut(s) 5, 10, 105, 289, 294, 373, 446, 475, 496
CviKI_1 RGCY 9 cut(s) 5, 10, 105, 289, 294, 373, 446, 475, 496
CviQI GTAC 1 cut(s) 130
DdeI CTNAG 2 cut(s) 6, 290
DpnI GATC 2 cut(s) 114, 311
DpnII GATC 2 cut(s) 112, 309
DriI GACNNNNNGTC 1 cut(s) 178
Eam1104I CTCTTC 1 cut(s) 465
Eam1105I GACNNNNNGTC 1 cut(s) 178
EarI CTCTTC 1 cut(s) 465
Eco130I CCWWGG 2 cut(s) 87, 97
Eco24I GRGCYC 1 cut(s) 12
Eco47I GGWCC 1 cut(s) 376
Eco57I CTGAAG 1 cut(s) 210
EcoRII CCWGG 2 cut(s) 324, 430
EcoT14I CCWWGG 2 cut(s) 87, 97
EcoT38I GRGCYC 1 cut(s) 12
ErhI CCWWGG 2 cut(s) 87, 97
FaeI CATG 3 cut(s) 101, 205, 507
FaqI GGGAC 1 cut(s) 247
FatI CATG 3 cut(s) 97, 201, 503
FbaI TGATCA 1 cut(s) 309
FblI GTMKAC 1 cut(s) 93
Fnu4HI GCNGC 1 cut(s) 447
FokI GGATG 3 cut(s) 209, 287, 413
FriOI GRGCYC 1 cut(s) 12
Fsp4HI GCNGC 1 cut(s) 447
GluI GCNGC 1 cut(s) 447
GsaI CCCAGC 1 cut(s) 234
HgaI GACGC 1 cut(s) 438
Hin1II CATG 3 cut(s) 101, 205, 507
HincII GTYRAC 2 cut(s) 166, 186
HindII GTYRAC 2 cut(s) 166, 186
HinfI GANTC 3 cut(s) 392, 439, 482
HphI GGTGA 5 cut(s) 59, 217, 353, 358, 477
Hpy166II GTNNAC 4 cut(s) 67, 94, 166, 186
Hpy188I TCNGA 1 cut(s) 314
Hpy188III TCNNGA 1 cut(s) 515
Hpy8I GTNNAC 4 cut(s) 67, 94, 166, 186
HpyAV CCTTC 2 cut(s) 116, 368
HpyCH4IV ACGT 2 cut(s) 63, 162
HpyCH4V TGCA 1 cut(s) 507
HpyF3I CTNAG 2 cut(s) 6, 290
HpySE526I ACGT 2 cut(s) 63, 162
Hsp92II CATG 3 cut(s) 101, 205, 507
Ksp22I TGATCA 1 cut(s) 309
Kzo9I GATC 2 cut(s) 112, 309
LguI GCTCTTC 1 cut(s) 465
LmnI GCTCC 1 cut(s) 370
Lsp1109I GCAGC 1 cut(s) 433
LweI GCATC 2 cut(s) 153, 222
MabI ACCWGGT 1 cut(s) 430
MaeII ACGT 2 cut(s) 63, 162
MaeIII GTNAC 1 cut(s) 149
MalI GATC 2 cut(s) 114, 311
MboI GATC 2 cut(s) 112, 309
MboII GAAGA 4 cut(s) 59, 401, 431, 482
MhlI GDGCHC 1 cut(s) 12
MluCI AATT 1 cut(s) 51
MlyI GAGTC 1 cut(s) 448
MmeI TCCRAC 1 cut(s) 416
MnlI CCTC 6 cut(s) 35, 36, 131, 201, 262, 265
MroXI GAANNNNTTC 1 cut(s) 51
MslI CAYNNNNRTG 1 cut(s) 508
MspR9I CCNGG 2 cut(s) 326, 432
MvaI CCWGG 2 cut(s) 326, 432
NcoI CCATGG 1 cut(s) 97
NdeII GATC 2 cut(s) 112, 309
NlaIII CATG 3 cut(s) 101, 205, 507
NlaIV GGNNCC 2 cut(s) 330, 378
NspI RCATGY 1 cut(s) 507
PciSI GCTCTTC 1 cut(s) 465
PdmI GAANNNNTTC 1 cut(s) 51
PfeI GAWTC 2 cut(s) 392, 482
PkrI GCNGC 1 cut(s) 448
PleI GAGTC 1 cut(s) 447
PpsI GAGTC 1 cut(s) 447
Psp1406I AACGTT 1 cut(s) 162
Psp6I CCWGG 2 cut(s) 324, 430
PspFI CCCAGC 1 cut(s) 230
PspGI CCWGG 2 cut(s) 324, 430
PspN4I GGNNCC 2 cut(s) 330, 378
PspPI GGNCC 1 cut(s) 376
RsaI GTAC 1 cut(s) 131
RsaNI GTAC 1 cut(s) 130
RseI CAYNNNNRTG 1 cut(s) 508
SapI GCTCTTC 1 cut(s) 465
SatI GCNGC 1 cut(s) 447
Sau3AI GATC 2 cut(s) 112, 309
Sau96I GGNCC 1 cut(s) 376
SchI GAGTC 1 cut(s) 448
ScrFI CCNGG 2 cut(s) 326, 432
SduI GDGCHC 1 cut(s) 12
SexAI ACCWGGT 1 cut(s) 430
SfaNI GCATC 2 cut(s) 153, 222
SinI GGWCC 1 cut(s) 376
SmiMI CAYNNNNRTG 1 cut(s) 508
Sse9I AATT 1 cut(s) 51
StyD4I CCNGG 2 cut(s) 324, 430
StyI CCWWGG 2 cut(s) 87, 97
TaiI ACGT 2 cut(s) 66, 165
TaqI TCGA 1 cut(s) 28
TasI AATT 1 cut(s) 51
TatI WGTACW 1 cut(s) 129
TfiI GAWTC 2 cut(s) 392, 482
TscAI CASTG 1 cut(s) 271
TseI GCWGC 1 cut(s) 446
TspDTI ATGAA 2 cut(s) 402, 432
TspRI CASTG 1 cut(s) 271
VpaK11BI GGWCC 1 cut(s) 376
XapI RAATTY 1 cut(s) 51
XceI RCATGY 1 cut(s) 507
XmiI GTMKAC 1 cut(s) 93
XmnI GAANNNNTTC 1 cut(s) 51
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.