MD05G1188500.v1.1

Photosystem II reaction center PSB28 protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0010122
Physical Location & Seq
Reverse (-)
31632863 .. 31634400
1538 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1188500.v1.1.491

Sequence Viewer

Length: 543 bp
ATGGCAACCCTGCAGTCGCTTGCATTCTCCTCTCCAGTCTCACAGTGTCTGCGGCAACCACGCCTCCATTCAGGCTTGTTATGTTGGGGTGTTCATAGAAGTGCAGAATCCACATTCAAAGGGCAGTCTTTGCGCGTGTCTCGTCCCCAATGGGCGCCATTAAGACGGTGTACTCAAGTGTCTAGGTCTGTTACAATGATGGTCAAACCGAAAATTCAGTTCATCCAAGGAACCGATGAACAGACAATACCAGATGTGAGGCTAACCAAATCAAAAGACGGTACAAATGGCGTGGCTATTTTCAGGTTTGAGCAACCTTCTGTGTTTGACTCCTCTGGTGAGTTTGGTGACATCACTGGATTTTACATGATTGACGAAGAAGGGGTCCTTTCGTCAGTTGATGTTAACGCCAAATTTGTCAACGGAAAGCCTGCAGGGATTGAAGCAAAGTATATAATGCGGACTCCAAGAGATTGGGACAGATTCATGAGATTCATGGAACGCTACTCAAATGACAATGGCTTGAGTTTCATAAAAAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00195 ko01100 map00195 map01100
Pfam Domains
Protein Families
Psb28

Protein Analysis

181

Amino Acids

20.4

Weight (kDa)

9.58

Isoelectric Point (pI)

48.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Psb28 PF03912 72 - 177 4e-42 Psb28 protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 154
AccII CGCG 1 cut(s) 135
AciI CCGC 2 cut(s) 52, 460
AcsI RAATTY 2 cut(s) 213, 413
AcyI GRCGYC 1 cut(s) 155
AfaI GTAC 2 cut(s) 172, 283
AgsI TTSAA 2 cut(s) 118, 443
Alw26I GTCTC 2 cut(s) 43, 144
AlwNI CAGNNNCTG 1 cut(s) 49
ApoI RAATTY 2 cut(s) 213, 413
ArsI GACNNNNNNTTYG 2 cut(s) 369, 401
AspLEI GCGC 2 cut(s) 135, 157
AspS9I GGNCC 1 cut(s) 385
AsuHPI GGTGA 2 cut(s) 350, 359
AvaII GGWCC 1 cut(s) 385
BanI GGYRCC 1 cut(s) 154
BccI CCATC 1 cut(s) 193
BcoDI GTCTC 2 cut(s) 43, 144
BfaI CTAG 1 cut(s) 183
BfmI CTRYAG 2 cut(s) 11, 432
BfoI RGCGCY 1 cut(s) 158
BisI GCNGC 1 cut(s) 53
BlsI GCNGC 1 cut(s) 54
Bme18I GGWCC 1 cut(s) 385
BmgT120I GGNCC 1 cut(s) 385
BmiI GGNNCC 3 cut(s) 156, 232, 386
BpmI CTGGAG 1 cut(s) 18
BpuEI CTTGAG 1 cut(s) 159
BsaHI GRCGYC 1 cut(s) 155
BsaJI CCNNGG 1 cut(s) 226
BsaXI ACNNNNNCTCC 2 cut(s) 48, 78
Bse1I ACTGG 2 cut(s) 35, 361
BseDI CCNNGG 1 cut(s) 226
BseGI GGATG 1 cut(s) 222
BseNI ACTGG 2 cut(s) 35, 361
BseRI GAGGAG 2 cut(s) 19, 322
BsgI GTGCAG 1 cut(s) 123
Bsh1236I CGCG 1 cut(s) 135
BshNI GGYRCC 1 cut(s) 154
BslFI GGGAC 2 cut(s) 129, 491
BsmAI GTCTC 2 cut(s) 43, 144
BsmFI GGGAC 2 cut(s) 129, 491
BsmI GAATGC 1 cut(s) 23
BspACI CCGC 2 cut(s) 52, 460
BspFNI CGCG 1 cut(s) 135
BspHI TCATGA 1 cut(s) 486
BspLI GGNNCC 3 cut(s) 156, 232, 386
BspMAI CTGCAG 2 cut(s) 15, 436
BspT107I GGYRCC 1 cut(s) 154
BsrI ACTGG 2 cut(s) 35, 361
BssECI CCNNGG 1 cut(s) 226
BssNI GRCGYC 1 cut(s) 155
BssT1I CCWWGG 1 cut(s) 226
Bst4CI ACNGT 3 cut(s) 45, 168, 281
BstACI GRCGYC 1 cut(s) 155
BstAPI GCANNNNNTGC 1 cut(s) 130
BstC8I GCNNGC 2 cut(s) 21, 432
BstF5I GGATG 1 cut(s) 222
BstFNI CGCG 1 cut(s) 135
BstH2I RGCGCY 1 cut(s) 158
BstHHI GCGC 2 cut(s) 135, 157
BstMAI GTCTC 2 cut(s) 43, 144
BstMWI GCNNNNNNNGC 1 cut(s) 130
BstSFI CTRYAG 2 cut(s) 11, 432
BstUI CGCG 1 cut(s) 135
BstXI CCANNNNNNTGG 1 cut(s) 474
BtsCI GGATG 1 cut(s) 222
BtsIMutI CAGTG 2 cut(s) 50, 354
Cac8I GCNNGC 2 cut(s) 21, 432
CaiI CAGNNNCTG 1 cut(s) 49
CciI TCATGA 1 cut(s) 486
CfoI GCGC 2 cut(s) 135, 157
Cfr13I GGNCC 1 cut(s) 385
Csp6I GTAC 2 cut(s) 171, 282
CspCI CAANNNNNGTGG 2 cut(s) 273, 308
CviAII CATG 3 cut(s) 367, 487, 496
CviJI RGCY 5 cut(s) 75, 262, 296, 430, 522
CviKI_1 RGCY 5 cut(s) 75, 262, 296, 430, 522
CviQI GTAC 2 cut(s) 171, 282
DinI GGCGCC 1 cut(s) 156
Eco130I CCWWGG 1 cut(s) 226
Eco47I GGWCC 1 cut(s) 385
EcoO109I RGGNCCY 1 cut(s) 385
EcoT14I CCWWGG 1 cut(s) 226
EgeI GGCGCC 1 cut(s) 156
EheI GGCGCC 1 cut(s) 156
ErhI CCWWGG 1 cut(s) 226
FaeI CATG 3 cut(s) 370, 490, 499
FaiI YATR 8 cut(s) 82, 96, 368, 453, 455, 488, 497, 533
FalI AAGNNNNNCTT 2 cut(s) 372, 404
FaqI GGGAC 2 cut(s) 129, 491
FatI CATG 3 cut(s) 366, 486, 495
Fnu4HI GCNGC 1 cut(s) 53
FokI GGATG 1 cut(s) 209
Fsp4HI GCNGC 1 cut(s) 53
FspBI CTAG 1 cut(s) 183
GlaI GCGC 2 cut(s) 134, 156
GluI GCNGC 1 cut(s) 53
GsuI CTGGAG 1 cut(s) 18
HaeII RGCGCY 1 cut(s) 158
HhaI GCGC 2 cut(s) 135, 157
Hin1I GRCGYC 1 cut(s) 155
Hin1II CATG 3 cut(s) 370, 490, 499
Hin6I GCGC 2 cut(s) 133, 155
HinP1I GCGC 2 cut(s) 133, 155
HincII GTYRAC 2 cut(s) 406, 421
HindII GTYRAC 2 cut(s) 406, 421
HinfI GANTC 5 cut(s) 107, 329, 463, 483, 492
HpaI GTTAAC 1 cut(s) 406
HphI GGTGA 2 cut(s) 350, 359
Hpy166II GTNNAC 3 cut(s) 171, 406, 421
Hpy188III TCNNGA 1 cut(s) 487
Hpy8I GTNNAC 3 cut(s) 171, 406, 421
HpyAV CCTTC 2 cut(s) 327, 374
HpyCH4III ACNGT 3 cut(s) 45, 168, 281
HpyCH4V TGCA 4 cut(s) 13, 23, 104, 434
HpyF10VI GCNNNNNNNGC 1 cut(s) 130
Hsp92I GRCGYC 1 cut(s) 155
Hsp92II CATG 3 cut(s) 370, 490, 499
HspAI GCGC 2 cut(s) 133, 155
KasI GGCGCC 1 cut(s) 154
KspAI GTTAAC 1 cut(s) 406
LpnPI CCDG 9 cut(s) 23, 48, 57, 264, 289, 321, 342, 420, 444
MaeI CTAG 1 cut(s) 183
MaeIII GTNAC 2 cut(s) 190, 347
MboII GAAGA 1 cut(s) 389
MluCI AATT 2 cut(s) 213, 413
Mly113I GGCGCC 1 cut(s) 155
MlyI GAGTC 2 cut(s) 323, 457
MnlI CCTC 4 cut(s) 40, 74, 252, 343
MseI TTAA 2 cut(s) 161, 405
MslI CAYNNNNRTG 1 cut(s) 99
Mva1269I GAATGC 1 cut(s) 23
MvnI CGCG 1 cut(s) 135
MwoI GCNNNNNNNGC 1 cut(s) 130
NarI GGCGCC 1 cut(s) 155
NlaIII CATG 3 cut(s) 370, 490, 499
NlaIV GGNNCC 3 cut(s) 156, 232, 386
NmuCI GTSAC 1 cut(s) 347
PagI TCATGA 1 cut(s) 486
PctI GAATGC 1 cut(s) 23
PfeI GAWTC 3 cut(s) 107, 483, 492
PkrI GCNGC 1 cut(s) 54
PleI GAGTC 2 cut(s) 323, 457
PluTI GGCGCC 1 cut(s) 158
PpsI GAGTC 2 cut(s) 323, 457
PpuMI RGGWCCY 1 cut(s) 385
Psp5II RGGWCCY 1 cut(s) 385
PspN4I GGNNCC 3 cut(s) 156, 232, 386
PspPI GGNCC 1 cut(s) 385
PspPPI RGGWCCY 1 cut(s) 385
PsrI GAACNNNNNNTAC 2 cut(s) 231, 263
PstI CTGCAG 2 cut(s) 15, 436
PstNI CAGNNNCTG 1 cut(s) 49
RsaI GTAC 2 cut(s) 172, 283
RsaNI GTAC 2 cut(s) 171, 282
RseI CAYNNNNRTG 1 cut(s) 99
SaqAI TTAA 2 cut(s) 161, 405
SatI GCNGC 1 cut(s) 53
Sau96I GGNCC 1 cut(s) 385
SbfI CCTGCAGG 1 cut(s) 436
SchI GAGTC 2 cut(s) 323, 457
SdaI CCTGCAGG 1 cut(s) 436
SetI ASST 3 cut(s) 188, 308, 319
SfcI CTRYAG 2 cut(s) 11, 432
SfoI GGCGCC 1 cut(s) 156
SinI GGWCC 1 cut(s) 385
SmiMI CAYNNNNRTG 1 cut(s) 99
SmlI CTYRAG 2 cut(s) 174, 523
SmoI CTYRAG 2 cut(s) 174, 523
Sse8387I CCTGCAGG 1 cut(s) 436
Sse9I AATT 2 cut(s) 213, 413
SsiI CCGC 2 cut(s) 52, 460
SspDI GGCGCC 1 cut(s) 154
SspMI CTAG 1 cut(s) 183
StyI CCWWGG 1 cut(s) 226
TaaI ACNGT 3 cut(s) 45, 168, 281
TasI AATT 2 cut(s) 213, 413
TatI WGTACW 1 cut(s) 170
TauI GCSGC 1 cut(s) 55
TfiI GAWTC 3 cut(s) 107, 483, 492
Tru1I TTAA 2 cut(s) 161, 405
Tru9I TTAA 2 cut(s) 161, 405
TscAI CASTG 2 cut(s) 50, 361
TseFI GTSAC 1 cut(s) 347
Tsp45I GTSAC 1 cut(s) 347
TspDTI ATGAA 6 cut(s) 83, 211, 252, 475, 484, 520
TspGWI ACGGA 1 cut(s) 438
TspRI CASTG 2 cut(s) 50, 361
VpaK11BI GGWCC 1 cut(s) 385
XapI RAATTY 2 cut(s) 213, 413
XspI CTAG 1 cut(s) 183
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.