Rroxscaffold_7G00182180

Photosystem II reaction center PSB28 protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0010122
Physical Location & Seq
Forward (+)
21187605 .. 21189061
1457 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00182180.1

Sequence Viewer

Length: 546 bp
ATGGCAACCTTGCAGTCTTCACTTGCATTCTCCTCTCCAGCCTCCCACTGTCTCCAGCAATCACGCTCTCATTCTGGGTTGTTATCTTGGGCGGTTCACAGAAGTGCAGGGTCCACATTCAATGGCCAGTCTTTGCGCATTTCTCGGCCCCAATGGGCGCCATTAAGGCAGTCTGCTCAAGCTTCTAGATCCATCACAATGATGGCGAAACCAAAAATTCAGTTTATCCAAGGAACTGATGAGCAGACAATACCAGATGTGAGGCTCACCAAATCGAAAGATGGTACCAATGGCGTGGCTATATTCAGGTTTGAGCAGCCTTCTGTTTTCGACTCATCTGACGAATTTGGTGATATCACTGGCTTTTACATGATTGATGAAGAAGGGGTTCTTTCATCTGTTGATGTTAATGCAAAATTTGTCAACGGAAAGCCTGCAGGAATTGAAGCAAAGTATACAATGCGGACACCGAGAGAATGGGACAGATTCATGAGATTCATGGAGCGCTATGCTAATGACAATGGCTTAAGTTTCATTAAAAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00195 ko01100 map00195 map01100
Pfam Domains
Protein Families
Psb28

Protein Analysis

181

Amino Acids

20.25

Weight (kDa)

9.37

Isoelectric Point (pI)

53.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Psb28 PF03912 73 - 178 4.4e-43 Psb28 protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 137
Acc65I GGTACC 1 cut(s) 284
AccB1I GGYRCC 2 cut(s) 157, 284
AccI GTMKAC 1 cut(s) 455
AciI CCGC 2 cut(s) 92, 463
AclWI GGATC 1 cut(s) 183
AcoI YGGCCR 1 cut(s) 124
AcsI RAATTY 3 cut(s) 216, 344, 416
AcyI GRCGYC 1 cut(s) 158
AfaI GTAC 1 cut(s) 286
AfeI AGCGCT 1 cut(s) 506
AflII CTTAAG 1 cut(s) 526
AgsI TTSAA 2 cut(s) 121, 446
AleI CACNNNNGTG 1 cut(s) 102
AluBI AGCT 1 cut(s) 182
AluI AGCT 1 cut(s) 182
Alw26I GTCTC 1 cut(s) 56
AlwI GGATC 1 cut(s) 183
Aor51HI AGCGCT 1 cut(s) 506
AoxI GGCC 2 cut(s) 124, 146
ApeKI GCWGC 1 cut(s) 316
ApoI RAATTY 3 cut(s) 216, 344, 416
Asp700I GAANNNNTTC 1 cut(s) 387
Asp718I GGTACC 1 cut(s) 284
AspLEI GCGC 3 cut(s) 138, 160, 507
AspS9I GGNCC 2 cut(s) 111, 147
AsuHPI GGTGA 2 cut(s) 259, 362
AvaII GGWCC 1 cut(s) 111
BalI TGGCCA 1 cut(s) 126
BanI GGYRCC 2 cut(s) 157, 284
BbsI GAAGAC 1 cut(s) 9
BbvI GCAGC 1 cut(s) 328
BccI CCATC 3 cut(s) 196, 200, 275
BcoDI GTCTC 1 cut(s) 56
BfaI CTAG 1 cut(s) 186
BfmI CTRYAG 1 cut(s) 435
BfoI RGCGCY 2 cut(s) 161, 508
BfrI CTTAAG 1 cut(s) 526
BglI GCCNNNNNGGC 1 cut(s) 166
BisI GCNGC 1 cut(s) 317
BlsI GCNGC 1 cut(s) 318
Bme18I GGWCC 1 cut(s) 111
BmgT120I GGNCC 2 cut(s) 111, 147
BmiI GGNNCC 4 cut(s) 112, 149, 159, 286
BpiI GAAGAC 1 cut(s) 9
BpmI CTGGAG 2 cut(s) 21, 38
BpuEI CTTGAG 1 cut(s) 162
BsaHI GRCGYC 1 cut(s) 158
BsaJI CCNNGG 1 cut(s) 229
Bse1I ACTGG 2 cut(s) 127, 364
BseDI CCNNGG 1 cut(s) 229
BseNI ACTGG 2 cut(s) 127, 364
BseRI GAGGAG 1 cut(s) 22
BseXI GCAGC 1 cut(s) 328
BsgI GTGCAG 1 cut(s) 126
BshFI GGCC 2 cut(s) 126, 148
BshNI GGYRCC 2 cut(s) 157, 284
BslFI GGGAC 1 cut(s) 494
BsmAI GTCTC 1 cut(s) 56
BsmFI GGGAC 1 cut(s) 494
BsmI GAATGC 1 cut(s) 26
BsnI GGCC 2 cut(s) 126, 148
Bsp143I GATC 1 cut(s) 188
BspACI CCGC 2 cut(s) 92, 463
BspANI GGCC 2 cut(s) 126, 148
BspHI TCATGA 1 cut(s) 489
BspLI GGNNCC 4 cut(s) 112, 149, 159, 286
BspMAI CTGCAG 1 cut(s) 439
BspPI GGATC 1 cut(s) 183
BspT107I GGYRCC 2 cut(s) 157, 284
BspTI CTTAAG 1 cut(s) 526
BsrI ACTGG 2 cut(s) 127, 364
BssECI CCNNGG 1 cut(s) 229
BssMI GATC 1 cut(s) 188
BssNAI GTATAC 1 cut(s) 456
BssNI GRCGYC 1 cut(s) 158
BssT1I CCWWGG 1 cut(s) 229
Bst1107I GTATAC 1 cut(s) 456
Bst4CI ACNGT 1 cut(s) 50
BstACI GRCGYC 1 cut(s) 158
BstAFI CTTAAG 1 cut(s) 526
BstC8I GCNNGC 1 cut(s) 435
BstH2I RGCGCY 2 cut(s) 161, 508
BstHHI GCGC 3 cut(s) 138, 160, 507
BstKTI GATC 1 cut(s) 191
BstMAI GTCTC 1 cut(s) 56
BstMBI GATC 1 cut(s) 188
BstMWI GCNNNNNNNGC 1 cut(s) 166
BstSFI CTRYAG 1 cut(s) 435
BstV1I GCAGC 1 cut(s) 328
BstV2I GAAGAC 1 cut(s) 9
BstX2I RGATCY 1 cut(s) 188
BstXI CCANNNNNNTGG 1 cut(s) 295
BstYI RGATCY 1 cut(s) 188
BstZ17I GTATAC 1 cut(s) 456
BsuRI GGCC 2 cut(s) 126, 148
BtsIMutI CAGTG 2 cut(s) 46, 357
Cac8I GCNNGC 1 cut(s) 435
CciI TCATGA 1 cut(s) 489
CfoI GCGC 3 cut(s) 138, 160, 507
Cfr13I GGNCC 2 cut(s) 111, 147
Csp6I GTAC 1 cut(s) 285
CviAII CATG 3 cut(s) 370, 490, 499
CviQI GTAC 1 cut(s) 285
DinI GGCGCC 1 cut(s) 159
DpnI GATC 1 cut(s) 190
DpnII GATC 1 cut(s) 188
EaeI YGGCCR 1 cut(s) 124
Eco130I CCWWGG 1 cut(s) 229
Eco32I GATATC 1 cut(s) 355
Eco47I GGWCC 1 cut(s) 111
Eco47III AGCGCT 1 cut(s) 506
EcoRV GATATC 1 cut(s) 355
EcoT14I CCWWGG 1 cut(s) 229
EgeI GGCGCC 1 cut(s) 159
EheI GGCGCC 1 cut(s) 159
ErhI CCWWGG 1 cut(s) 229
FaeI CATG 3 cut(s) 373, 493, 502
FaiI YATR 6 cut(s) 302, 371, 456, 491, 500, 510
FalI AAGNNNNNCTT 2 cut(s) 375, 407
FaqI GGGAC 1 cut(s) 494
FatI CATG 3 cut(s) 369, 489, 498
FblI GTMKAC 1 cut(s) 455
Fnu4HI GCNGC 1 cut(s) 317
Fsp4HI GCNGC 1 cut(s) 317
FspBI CTAG 1 cut(s) 186
FspI TGCGCA 1 cut(s) 137
GlaI GCGC 3 cut(s) 137, 159, 506
GluI GCNGC 1 cut(s) 317
GsuI CTGGAG 2 cut(s) 21, 38
HaeII RGCGCY 2 cut(s) 161, 508
HaeIII GGCC 2 cut(s) 126, 148
HhaI GCGC 3 cut(s) 138, 160, 507
Hin1I GRCGYC 1 cut(s) 158
Hin1II CATG 3 cut(s) 373, 493, 502
Hin6I GCGC 3 cut(s) 136, 158, 505
HinP1I GCGC 3 cut(s) 136, 158, 505
HincII GTYRAC 1 cut(s) 424
HindII GTYRAC 1 cut(s) 424
HindIII AAGCTT 1 cut(s) 180
HinfI GANTC 3 cut(s) 332, 486, 495
HphI GGTGA 2 cut(s) 259, 362
Hpy166II GTNNAC 4 cut(s) 97, 114, 424, 456
Hpy188I TCNGA 1 cut(s) 340
Hpy188III TCNNGA 2 cut(s) 186, 490
Hpy8I GTNNAC 4 cut(s) 97, 114, 424, 456
HpyAV CCTTC 2 cut(s) 330, 377
HpyCH4III ACNGT 1 cut(s) 50
HpyCH4V TGCA 5 cut(s) 13, 26, 107, 413, 437
HpyF10VI GCNNNNNNNGC 1 cut(s) 166
Hsp92I GRCGYC 1 cut(s) 158
Hsp92II CATG 3 cut(s) 373, 493, 502
HspAI GCGC 3 cut(s) 136, 158, 505
KasI GGCGCC 1 cut(s) 157
KpnI GGTACC 1 cut(s) 288
Kzo9I GATC 1 cut(s) 188
LmnI GCTCC 1 cut(s) 502
Lsp1109I GCAGC 1 cut(s) 328
MaeI CTAG 1 cut(s) 186
MalI GATC 1 cut(s) 190
MboI GATC 1 cut(s) 188
MboII GAAGA 2 cut(s) 9, 392
MflI RGATCY 1 cut(s) 188
MlsI TGGCCA 1 cut(s) 126
MluCI AATT 4 cut(s) 216, 344, 416, 441
MluNI TGGCCA 1 cut(s) 126
Mly113I GGCGCC 1 cut(s) 158
MlyI GAGTC 1 cut(s) 326
MnlI CCTC 3 cut(s) 43, 52, 255
Mox20I TGGCCA 1 cut(s) 126
MroXI GAANNNNTTC 1 cut(s) 387
MscI TGGCCA 1 cut(s) 126
MseI TTAA 4 cut(s) 164, 408, 527, 537
MslI CAYNNNNRTG 3 cut(s) 102, 197, 200
Msp20I TGGCCA 1 cut(s) 126
MspCI CTTAAG 1 cut(s) 526
Mva1269I GAATGC 1 cut(s) 26
MwoI GCNNNNNNNGC 1 cut(s) 166
NarI GGCGCC 1 cut(s) 158
NdeII GATC 1 cut(s) 188
NlaIII CATG 3 cut(s) 373, 493, 502
NlaIV GGNNCC 4 cut(s) 112, 149, 159, 286
NmeAIII GCCGAG 1 cut(s) 124
NsbI TGCGCA 1 cut(s) 137
OliI CACNNNNGTG 1 cut(s) 102
PagI TCATGA 1 cut(s) 489
PctI GAATGC 1 cut(s) 26
PdmI GAANNNNTTC 1 cut(s) 387
PfeI GAWTC 2 cut(s) 486, 495
PkrI GCNGC 1 cut(s) 318
PleI GAGTC 1 cut(s) 326
PluTI GGCGCC 1 cut(s) 161
PpsI GAGTC 1 cut(s) 326
PspN4I GGNNCC 4 cut(s) 112, 149, 159, 286
PspPI GGNCC 2 cut(s) 111, 147
PstI CTGCAG 1 cut(s) 439
PsuI RGATCY 1 cut(s) 188
RsaI GTAC 1 cut(s) 286
RsaNI GTAC 1 cut(s) 285
RseI CAYNNNNRTG 3 cut(s) 102, 197, 200
SaqAI TTAA 4 cut(s) 164, 408, 527, 537
SatI GCNGC 1 cut(s) 317
Sau3AI GATC 1 cut(s) 188
Sau96I GGNCC 2 cut(s) 111, 147
SbfI CCTGCAGG 1 cut(s) 439
SchI GAGTC 1 cut(s) 326
SdaI CCTGCAGG 1 cut(s) 439
SetI ASST 3 cut(s) 11, 184, 311
SfcI CTRYAG 1 cut(s) 435
SfoI GGCGCC 1 cut(s) 159
SinI GGWCC 1 cut(s) 111
SmiMI CAYNNNNRTG 3 cut(s) 102, 197, 200
SmlI CTYRAG 2 cut(s) 177, 526
SmoI CTYRAG 2 cut(s) 177, 526
Sse8387I CCTGCAGG 1 cut(s) 439
Sse9I AATT 4 cut(s) 216, 344, 416, 441
SsiI CCGC 2 cut(s) 92, 463
SspDI GGCGCC 1 cut(s) 157
SspMI CTAG 1 cut(s) 186
StyI CCWWGG 1 cut(s) 229
TaaI ACNGT 1 cut(s) 50
TaqI TCGA 2 cut(s) 275, 330
TasI AATT 4 cut(s) 216, 344, 416, 441
TfiI GAWTC 2 cut(s) 486, 495
Tru1I TTAA 4 cut(s) 164, 408, 527, 537
Tru9I TTAA 4 cut(s) 164, 408, 527, 537
TscAI CASTG 2 cut(s) 53, 364
TseI GCWGC 1 cut(s) 316
TspDTI ATGAA 5 cut(s) 384, 393, 478, 487, 523
TspGWI ACGGA 1 cut(s) 441
TspRI CASTG 2 cut(s) 53, 364
Vha464I CTTAAG 1 cut(s) 526
VpaK11BI GGWCC 1 cut(s) 111
XapI RAATTY 3 cut(s) 216, 344, 416
XbaI TCTAGA 1 cut(s) 185
XcmI CCANNNNNNNNNTGG 1 cut(s) 199
XmiI GTMKAC 1 cut(s) 455
XmnI GAANNNNTTC 1 cut(s) 387
XspI CTAG 1 cut(s) 186
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.