AT4G28660

Photosystem II reaction center Psb28 protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0010122
Physical Location & Seq
Forward (+)
14149849 .. 14151163
1315 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G28660.1

Sequence Viewer

Length: 552 bp
ATGGCTTGTGTTCGGTCGATTGGTTCTTTAACTTCTGTCAACCATTCTCAACGAAATGTTCCTCGCTCAGGCATTGTCTTATCTTCATGCTCTGTTCTTCCCATCAAAAGCTCATCTTTCACTGGATCCCCTGTTTCTCTCCCGCGGGCTCAACCATCATCCCGAACAGCTCTAAGACCGATGAAACCCGTCTCTATCACGATGATGGTAAAGCCGGCGCTCCAATTCATCCAAGGGACGGATGAGATGACAATCCCTGATGTTAAGCTGACTCGATCAAGAGACGGAAGCAACGGAATGGCACTGTTCTCTTTCGACCAACCATCCGTTTTCGACTCGAGCGGTGAAGTCGGAGAGATTACAGGATTGTACATGATTGATGAGGAAGGTGTGATTCAGTCGACGGATGTGAACGCGAGATTCGTGAATGGGAAACCGGAAGGGATTGTGGCGAAGCACATAATGCGATCGCCGAAAGAATGGGACAGGTTCATGAGGTTTATGGAGCGATACTCGGACCAGAATGGCTTGCAGTTTGTTAAGAAGCAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00195 ko01100 map00195 map01100
Pfam Domains
Protein Families
Psb28

Protein Analysis

183

Amino Acids

20.19

Weight (kDa)

9.46

Isoelectric Point (pI)

53.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Psb28 PF03912 74 - 179 2.5e-42 Psb28 protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 342
AccI GTMKAC 1 cut(s) 401
AccII CGCG 2 cut(s) 145, 416
AciI CCGC 3 cut(s) 143, 145, 342
AclWI GGATC 2 cut(s) 120, 133
AfaI GTAC 1 cut(s) 371
AfiI CCNNNNNNNGG 2 cut(s) 68, 238
AluBI AGCT 3 cut(s) 111, 170, 268
AluI AGCT 3 cut(s) 111, 170, 268
Alw26I GTCTC 2 cut(s) 196, 276
AlwI GGATC 2 cut(s) 120, 133
Ama87I CYCGRG 1 cut(s) 337
AsiSI GCGATCGC 1 cut(s) 470
AspLEI GCGC 1 cut(s) 220
AspS9I GGNCC 1 cut(s) 517
AsuHPI GGTGA 1 cut(s) 356
AvaI CYCGRG 1 cut(s) 337
AvaII GGWCC 1 cut(s) 517
BamHI GGATCC 1 cut(s) 125
BanII GRGCYC 1 cut(s) 151
BccI CCATC 4 cut(s) 110, 163, 199, 331
BcoDI GTCTC 2 cut(s) 196, 276
BfoI RGCGCY 1 cut(s) 221
Bme18I GGWCC 1 cut(s) 517
BmeT110I CYCGRG 1 cut(s) 337
BmgT120I GGNCC 1 cut(s) 517
BmiI GGNNCC 1 cut(s) 127
BplI GAGNNNNNCTC 2 cut(s) 497, 529
Bpu10I CCTNAGC 1 cut(s) 67
BsaBI GATNNNNATC 1 cut(s) 251
BsaJI CCNNGG 2 cut(s) 143, 232
BsaWI WCCGGW 1 cut(s) 436
Bsc4I CCNNNNNNNGG 2 cut(s) 68, 238
Bse118I RCCGGY 1 cut(s) 214
Bse1I ACTGG 1 cut(s) 127
Bse8I GATNNNNATC 1 cut(s) 251
BseDI CCNNGG 2 cut(s) 143, 232
BseGI GGATG 5 cut(s) 158, 228, 247, 323, 412
BseJI GATNNNNATC 1 cut(s) 251
BseLI CCNNNNNNNGG 2 cut(s) 68, 238
BseMII CTCAG 1 cut(s) 81
BseNI ACTGG 1 cut(s) 127
Bsh1236I CGCG 2 cut(s) 145, 416
Bsh1285I CGRYCG 2 cut(s) 17, 470
BsiEI CGRYCG 2 cut(s) 17, 470
BsiHKCI CYCGRG 1 cut(s) 337
BsiSI CCGG 2 cut(s) 215, 437
BslFI GGGAC 2 cut(s) 250, 497
BslI CCNNNNNNNGG 2 cut(s) 68, 238
BsmAI GTCTC 2 cut(s) 196, 276
BsmBI CGTCTC 2 cut(s) 196, 276
BsmFI GGGAC 2 cut(s) 250, 497
BsoBI CYCGRG 1 cut(s) 337
Bsp1286I GDGCHC 1 cut(s) 151
Bsp1407I TGTACA 1 cut(s) 369
Bsp143I GATC 3 cut(s) 125, 275, 467
BspACI CCGC 3 cut(s) 143, 145, 342
BspCNI CTCAG 1 cut(s) 80
BspFNI CGCG 2 cut(s) 145, 416
BspHI TCATGA 1 cut(s) 492
BspLI GGNNCC 1 cut(s) 127
BspPI GGATC 2 cut(s) 120, 133
BsrBI CCGCTC 1 cut(s) 342
BsrFI RCCGGY 1 cut(s) 214
BsrGI TGTACA 1 cut(s) 369
BsrI ACTGG 1 cut(s) 127
BssAI RCCGGY 1 cut(s) 214
BssECI CCNNGG 2 cut(s) 143, 232
BssMI GATC 3 cut(s) 125, 275, 467
BssT1I CCWWGG 1 cut(s) 232
Bst4CI ACNGT 1 cut(s) 306
BstAPI GCANNNNNTGC 1 cut(s) 463
BstAUI TGTACA 1 cut(s) 369
BstC8I GCNNGC 3 cut(s) 147, 216, 530
BstDEI CTNAG 2 cut(s) 67, 173
BstDSI CCRYGG 1 cut(s) 143
BstENI CCTNNNNNAGG 1 cut(s) 66
BstF5I GGATG 5 cut(s) 158, 228, 247, 323, 412
BstFNI CGCG 2 cut(s) 145, 416
BstH2I RGCGCY 1 cut(s) 221
BstHHI GCGC 1 cut(s) 220
BstKTI GATC 3 cut(s) 128, 278, 470
BstMAI GTCTC 2 cut(s) 196, 276
BstMBI GATC 3 cut(s) 125, 275, 467
BstMCI CGRYCG 2 cut(s) 17, 470
BstMWI GCNNNNNNNGC 1 cut(s) 463
BstUI CGCG 2 cut(s) 145, 416
BstX2I RGATCY 1 cut(s) 125
BstYI RGATCY 1 cut(s) 125
BtgI CCRYGG 1 cut(s) 143
BtsCI GGATG 5 cut(s) 158, 228, 247, 323, 412
BtsIMutI CAGTG 2 cut(s) 120, 302
Cac8I GCNNGC 3 cut(s) 147, 216, 530
CciI TCATGA 1 cut(s) 492
CfoI GCGC 1 cut(s) 220
Cfr10I RCCGGY 1 cut(s) 214
Cfr13I GGNCC 1 cut(s) 517
Cfr42I CCGCGG 1 cut(s) 146
Csp6I GTAC 1 cut(s) 370
CviAII CATG 3 cut(s) 87, 373, 493
CviJI RGCY 7 cut(s) 5, 111, 149, 170, 214, 268, 528
CviKI_1 RGCY 7 cut(s) 5, 111, 149, 170, 214, 268, 528
CviQI GTAC 1 cut(s) 370
DdeI CTNAG 2 cut(s) 67, 173
DpnI GATC 3 cut(s) 127, 277, 469
DpnII GATC 3 cut(s) 125, 275, 467
Eco130I CCWWGG 1 cut(s) 232
Eco24I GRGCYC 1 cut(s) 151
Eco47I GGWCC 1 cut(s) 517
Eco88I CYCGRG 1 cut(s) 337
EcoNI CCTNNNNNAGG 1 cut(s) 66
EcoT14I CCWWGG 1 cut(s) 232
EcoT38I GRGCYC 1 cut(s) 151
ErhI CCWWGG 1 cut(s) 232
Esp3I CGTCTC 2 cut(s) 196, 276
FaeI CATG 3 cut(s) 90, 376, 496
FaiI YATR 5 cut(s) 88, 374, 461, 494, 503
FalI AAGNNNNNCTT 2 cut(s) 100, 132
FaqI GGGAC 2 cut(s) 250, 497
FatI CATG 3 cut(s) 86, 372, 492
FauI CCCGC 2 cut(s) 138, 150
FblI GTMKAC 1 cut(s) 401
FokI GGATG 5 cut(s) 145, 215, 254, 310, 419
FriOI GRGCYC 1 cut(s) 151
GlaI GCGC 1 cut(s) 219
HaeII RGCGCY 1 cut(s) 221
HapII CCGG 2 cut(s) 215, 437
HhaI GCGC 1 cut(s) 220
Hin1II CATG 3 cut(s) 90, 376, 496
Hin6I GCGC 1 cut(s) 218
HinP1I GCGC 1 cut(s) 218
HincII GTYRAC 2 cut(s) 40, 402
HindII GTYRAC 2 cut(s) 40, 402
HinfI GANTC 4 cut(s) 271, 335, 394, 420
HpaII CCGG 2 cut(s) 215, 437
HphI GGTGA 1 cut(s) 356
Hpy166II GTNNAC 3 cut(s) 40, 402, 412
Hpy188I TCNGA 2 cut(s) 353, 517
Hpy188III TCNNGA 5 cut(s) 162, 199, 279, 424, 493
Hpy8I GTNNAC 3 cut(s) 40, 402, 412
Hpy99I CGWCG 1 cut(s) 406
HpyAV CCTTC 2 cut(s) 380, 434
HpyCH4III ACNGT 1 cut(s) 306
HpyCH4V TGCA 1 cut(s) 532
HpyF10VI GCNNNNNNNGC 1 cut(s) 463
HpyF3I CTNAG 2 cut(s) 67, 173
Hsp92II CATG 3 cut(s) 90, 376, 496
HspAI GCGC 1 cut(s) 218
KroI GCCGGC 1 cut(s) 214
KroNI GCCGGC 1 cut(s) 216
KspI CCGCGG 1 cut(s) 146
Kzo9I GATC 3 cut(s) 125, 275, 467
LmnI GCTCC 2 cut(s) 225, 505
LpnPI CCDG 9 cut(s) 54, 108, 144, 228, 270, 348, 450, 472, 533
MalI GATC 3 cut(s) 127, 277, 469
MbiI CCGCTC 1 cut(s) 342
MboI GATC 3 cut(s) 125, 275, 467
MboII GAAGA 2 cut(s) 75, 89
MflI RGATCY 1 cut(s) 125
MhlI GDGCHC 1 cut(s) 151
MluCI AATT 1 cut(s) 224
MlyI GAGTC 2 cut(s) 265, 329
MmeI TCCRAC 1 cut(s) 331
MnlI CCTC 3 cut(s) 72, 376, 489
MroNI GCCGGC 1 cut(s) 214
MseI TTAA 3 cut(s) 29, 264, 540
MslI CAYNNNNRTG 1 cut(s) 203
MspA1I CMGCKG 1 cut(s) 145
MspI CCGG 2 cut(s) 215, 437
MvnI CGCG 2 cut(s) 145, 416
MwoI GCNNNNNNNGC 1 cut(s) 463
NaeI GCCGGC 1 cut(s) 216
NdeII GATC 3 cut(s) 125, 275, 467
NgoMIV GCCGGC 1 cut(s) 214
NlaIII CATG 3 cut(s) 90, 376, 496
NlaIV GGNNCC 1 cut(s) 127
PaeR7I CTCGAG 1 cut(s) 337
PagI TCATGA 1 cut(s) 492
PcsI WCGNNNNNNNCGW 1 cut(s) 420
PdiI GCCGGC 1 cut(s) 216
PfeI GAWTC 2 cut(s) 394, 420
Ple19I CGATCG 1 cut(s) 470
PleI GAGTC 2 cut(s) 265, 329
PpsI GAGTC 2 cut(s) 265, 329
PspN4I GGNNCC 1 cut(s) 127
PspPI GGNCC 1 cut(s) 517
PspXI VCTCGAGB 1 cut(s) 337
PsuI RGATCY 1 cut(s) 125
PvuI CGATCG 1 cut(s) 470
RgaI GCGATCGC 1 cut(s) 470
RsaI GTAC 1 cut(s) 371
RsaNI GTAC 1 cut(s) 370
RseI CAYNNNNRTG 1 cut(s) 203
SacII CCGCGG 1 cut(s) 146
SalI GTCGAC 1 cut(s) 400
SaqAI TTAA 3 cut(s) 29, 264, 540
Sau3AI GATC 3 cut(s) 125, 275, 467
Sau96I GGNCC 1 cut(s) 517
SchI GAGTC 2 cut(s) 265, 329
SduI GDGCHC 1 cut(s) 151
SetI ASST 6 cut(s) 113, 172, 270, 391, 491, 500
SfaAI GCGATCGC 1 cut(s) 470
Sfr274I CTCGAG 1 cut(s) 337
Sfr303I CCGCGG 1 cut(s) 146
SgfI GCGATCGC 1 cut(s) 470
SgrBI CCGCGG 1 cut(s) 146
SinI GGWCC 1 cut(s) 517
SlaI CTCGAG 1 cut(s) 337
SmiMI CAYNNNNRTG 1 cut(s) 203
SmlI CTYRAG 1 cut(s) 337
SmoI CTYRAG 1 cut(s) 337
Sse9I AATT 1 cut(s) 224
SsiI CCGC 3 cut(s) 143, 145, 342
StyI CCWWGG 1 cut(s) 232
TaaI ACNGT 1 cut(s) 306
TaqI TCGA 6 cut(s) 17, 274, 315, 333, 338, 401
TaqII GACCGA 1 cut(s) 193
TasI AATT 1 cut(s) 224
TatI WGTACW 1 cut(s) 369
TfiI GAWTC 2 cut(s) 394, 420
Tru1I TTAA 3 cut(s) 29, 264, 540
Tru9I TTAA 3 cut(s) 29, 264, 540
TscAI CASTG 2 cut(s) 127, 309
TspDTI ATGAA 4 cut(s) 75, 197, 217, 481
TspGWI ACGGA 5 cut(s) 254, 300, 309, 316, 419
TspRI CASTG 2 cut(s) 127, 309
VpaK11BI GGWCC 1 cut(s) 517
XagI CCTNNNNNAGG 1 cut(s) 66
XhoI CTCGAG 1 cut(s) 337
XmiI GTMKAC 1 cut(s) 401
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.