FvH4_2g20470

Photosystem II reaction center PSB28 protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0010122
Physical Location & Seq
Reverse (-)
17180693 .. 17182268
1576 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g20470.t1

Sequence Viewer

Length: 546 bp
ATGGCAACCTTGCAGTCTTCACTTGCATTCTCCTCTCCAGCCTCCCACTGTCTCCAGCAGTCACGCTCTCATTCTGGGTTGTTATCATGGGCGGTTCATAGAAGTGCCGAGTCCACATTCAATGGCCAGTCTTTGCGCATTTCTCGGCCCCAATGGGTGCCACTAAGGCGGTCTATTCAAGCTTCTAGGTCCATCACAATGATGGCGAAAACAAAAATTCAGTTTATCCAAGGAACTGATGAGCAGACAATACCAGATGTGAGGCTAACCAAATCGAAAGATGGTACCAATGGCGTGGCTACATTCAGGTTTGAGCAGCCTTCTGTTTTCGACTCATCTGGCGAATTTGGTGATATCACTGGCTTTTACATGATTGATGAAGAAGGGGTCCTTTCATCTGTTGATGTTAACGCAAAATTTGTAAACGGAAAGCCTGCAGGAATTGAAGCAAAGTATATCATGCGGACACCAAGAGAATGGGACAGATTTATGAGATTCATGGAGCGCTATGCTAATGACAACGGCTTGAGTTTCATTAAAAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00195 ko01100 map00195 map01100
Pfam Domains
Protein Families
Psb28

Protein Analysis

182

Amino Acids

20.37

Weight (kDa)

9.56

Isoelectric Point (pI)

58.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Psb28 PF03912 73 - 178 5.5e-43 Psb28 protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 137
Acc65I GGTACC 1 cut(s) 284
AccB1I GGYRCC 2 cut(s) 157, 284
AciI CCGC 3 cut(s) 92, 169, 463
AcoI YGGCCR 1 cut(s) 124
AcsI RAATTY 3 cut(s) 216, 344, 416
AfaI GTAC 1 cut(s) 286
AfeI AGCGCT 1 cut(s) 506
AgsI TTSAA 3 cut(s) 121, 179, 446
AluBI AGCT 1 cut(s) 182
AluI AGCT 1 cut(s) 182
Alw26I GTCTC 1 cut(s) 56
Aor51HI AGCGCT 1 cut(s) 506
AoxI GGCC 2 cut(s) 124, 146
ApeKI GCWGC 1 cut(s) 316
ApoI RAATTY 3 cut(s) 216, 344, 416
Asp718I GGTACC 1 cut(s) 284
AspLEI GCGC 2 cut(s) 138, 507
AspS9I GGNCC 3 cut(s) 147, 189, 388
AsuHPI GGTGA 1 cut(s) 362
AvaII GGWCC 2 cut(s) 189, 388
BalI TGGCCA 1 cut(s) 126
BanI GGYRCC 2 cut(s) 157, 284
BbsI GAAGAC 1 cut(s) 9
BbvI GCAGC 1 cut(s) 328
BccI CCATC 3 cut(s) 196, 200, 275
BceAI ACGGC 1 cut(s) 538
BcoDI GTCTC 1 cut(s) 56
BfaI CTAG 1 cut(s) 186
BfmI CTRYAG 1 cut(s) 435
BfoI RGCGCY 1 cut(s) 508
BglI GCCNNNNNGGC 1 cut(s) 166
BisI GCNGC 1 cut(s) 317
BlsI GCNGC 1 cut(s) 318
Bme18I GGWCC 2 cut(s) 189, 388
BmgT120I GGNCC 3 cut(s) 147, 189, 388
BmiI GGNNCC 4 cut(s) 149, 159, 286, 389
BpiI GAAGAC 1 cut(s) 9
BpmI CTGGAG 2 cut(s) 21, 38
BsaJI CCNNGG 1 cut(s) 229
Bse1I ACTGG 2 cut(s) 127, 364
BseDI CCNNGG 1 cut(s) 229
BseNI ACTGG 2 cut(s) 127, 364
BseRI GAGGAG 1 cut(s) 22
BseXI GCAGC 1 cut(s) 328
BshFI GGCC 2 cut(s) 126, 148
BshNI GGYRCC 2 cut(s) 157, 284
BslFI GGGAC 1 cut(s) 494
BsmAI GTCTC 1 cut(s) 56
BsmFI GGGAC 1 cut(s) 494
BsmI GAATGC 1 cut(s) 26
BsnI GGCC 2 cut(s) 126, 148
BspACI CCGC 3 cut(s) 92, 169, 463
BspANI GGCC 2 cut(s) 126, 148
BspLI GGNNCC 4 cut(s) 149, 159, 286, 389
BspMAI CTGCAG 1 cut(s) 439
BspT107I GGYRCC 2 cut(s) 157, 284
BsrI ACTGG 2 cut(s) 127, 364
BssECI CCNNGG 1 cut(s) 229
BssT1I CCWWGG 1 cut(s) 229
Bst4CI ACNGT 1 cut(s) 50
BstC8I GCNNGC 1 cut(s) 435
BstDEI CTNAG 1 cut(s) 164
BstH2I RGCGCY 1 cut(s) 508
BstHHI GCGC 2 cut(s) 138, 507
BstMAI GTCTC 1 cut(s) 56
BstMWI GCNNNNNNNGC 1 cut(s) 166
BstSFI CTRYAG 1 cut(s) 435
BstV1I GCAGC 1 cut(s) 328
BstV2I GAAGAC 1 cut(s) 9
BstXI CCANNNNNNTGG 2 cut(s) 295, 477
BsuRI GGCC 2 cut(s) 126, 148
BtsIMutI CAGTG 2 cut(s) 46, 357
Cac8I GCNNGC 1 cut(s) 435
CfoI GCGC 2 cut(s) 138, 507
Cfr13I GGNCC 3 cut(s) 147, 189, 388
Csp6I GTAC 1 cut(s) 285
CviAII CATG 4 cut(s) 87, 370, 460, 499
CviQI GTAC 1 cut(s) 285
DdeI CTNAG 1 cut(s) 164
EaeI YGGCCR 1 cut(s) 124
Eco130I CCWWGG 1 cut(s) 229
Eco32I GATATC 1 cut(s) 355
Eco47I GGWCC 2 cut(s) 189, 388
Eco47III AGCGCT 1 cut(s) 506
EcoO109I RGGNCCY 1 cut(s) 388
EcoRV GATATC 1 cut(s) 355
EcoT14I CCWWGG 1 cut(s) 229
ErhI CCWWGG 1 cut(s) 229
FaeI CATG 4 cut(s) 90, 373, 463, 502
FaiI YATR 8 cut(s) 88, 99, 371, 456, 461, 491, 500, 510
FalI AAGNNNNNCTT 2 cut(s) 375, 407
FaqI GGGAC 1 cut(s) 494
FatI CATG 4 cut(s) 86, 369, 459, 498
Fnu4HI GCNGC 1 cut(s) 317
Fsp4HI GCNGC 1 cut(s) 317
FspBI CTAG 1 cut(s) 186
FspI TGCGCA 1 cut(s) 137
GlaI GCGC 2 cut(s) 137, 506
GluI GCNGC 1 cut(s) 317
GsuI CTGGAG 2 cut(s) 21, 38
HaeII RGCGCY 1 cut(s) 508
HaeIII GGCC 2 cut(s) 126, 148
HhaI GCGC 2 cut(s) 138, 507
Hin1II CATG 4 cut(s) 90, 373, 463, 502
Hin6I GCGC 2 cut(s) 136, 505
HinP1I GCGC 2 cut(s) 136, 505
HincII GTYRAC 1 cut(s) 409
HindII GTYRAC 1 cut(s) 409
HindIII AAGCTT 1 cut(s) 180
HinfI GANTC 3 cut(s) 110, 332, 495
HpaI GTTAAC 1 cut(s) 409
HphI GGTGA 1 cut(s) 362
Hpy166II GTNNAC 3 cut(s) 114, 409, 424
Hpy8I GTNNAC 3 cut(s) 114, 409, 424
HpyAV CCTTC 2 cut(s) 330, 377
HpyCH4III ACNGT 1 cut(s) 50
HpyCH4V TGCA 3 cut(s) 13, 26, 437
HpyF10VI GCNNNNNNNGC 1 cut(s) 166
HpyF3I CTNAG 1 cut(s) 164
Hsp92II CATG 4 cut(s) 90, 373, 463, 502
HspAI GCGC 2 cut(s) 136, 505
KpnI GGTACC 1 cut(s) 288
KspAI GTTAAC 1 cut(s) 409
LmnI GCTCC 1 cut(s) 502
Lsp1109I GCAGC 1 cut(s) 328
MaeI CTAG 1 cut(s) 186
MaeIII GTNAC 1 cut(s) 60
MboII GAAGA 2 cut(s) 9, 392
MlsI TGGCCA 1 cut(s) 126
MluCI AATT 4 cut(s) 216, 344, 416, 441
MluNI TGGCCA 1 cut(s) 126
MlyI GAGTC 2 cut(s) 119, 326
MnlI CCTC 3 cut(s) 43, 52, 255
Mox20I TGGCCA 1 cut(s) 126
MscI TGGCCA 1 cut(s) 126
MseI TTAA 2 cut(s) 408, 537
MslI CAYNNNNRTG 3 cut(s) 102, 197, 200
Msp20I TGGCCA 1 cut(s) 126
Mva1269I GAATGC 1 cut(s) 26
MwoI GCNNNNNNNGC 1 cut(s) 166
NlaIII CATG 4 cut(s) 90, 373, 463, 502
NlaIV GGNNCC 4 cut(s) 149, 159, 286, 389
NmeAIII GCCGAG 2 cut(s) 124, 133
NmuCI GTSAC 1 cut(s) 60
NsbI TGCGCA 1 cut(s) 137
PctI GAATGC 1 cut(s) 26
PfeI GAWTC 1 cut(s) 495
PkrI GCNGC 1 cut(s) 318
PleI GAGTC 2 cut(s) 118, 326
PpsI GAGTC 2 cut(s) 118, 326
PpuMI RGGWCCY 1 cut(s) 388
Psp5II RGGWCCY 1 cut(s) 388
PspN4I GGNNCC 4 cut(s) 149, 159, 286, 389
PspPI GGNCC 3 cut(s) 147, 189, 388
PspPPI RGGWCCY 1 cut(s) 388
PstI CTGCAG 1 cut(s) 439
RsaI GTAC 1 cut(s) 286
RsaNI GTAC 1 cut(s) 285
RseI CAYNNNNRTG 3 cut(s) 102, 197, 200
SaqAI TTAA 2 cut(s) 408, 537
SatI GCNGC 1 cut(s) 317
Sau96I GGNCC 3 cut(s) 147, 189, 388
SbfI CCTGCAGG 1 cut(s) 439
SchI GAGTC 2 cut(s) 119, 326
SdaI CCTGCAGG 1 cut(s) 439
SetI ASST 4 cut(s) 11, 184, 191, 311
SfcI CTRYAG 1 cut(s) 435
SinI GGWCC 2 cut(s) 189, 388
SmiMI CAYNNNNRTG 3 cut(s) 102, 197, 200
SmlI CTYRAG 1 cut(s) 526
SmoI CTYRAG 1 cut(s) 526
Sse8387I CCTGCAGG 1 cut(s) 439
Sse9I AATT 4 cut(s) 216, 344, 416, 441
SsiI CCGC 3 cut(s) 92, 169, 463
SspMI CTAG 1 cut(s) 186
StyI CCWWGG 1 cut(s) 229
TaaI ACNGT 1 cut(s) 50
TaqI TCGA 2 cut(s) 275, 330
TasI AATT 4 cut(s) 216, 344, 416, 441
TfiI GAWTC 1 cut(s) 495
Tru1I TTAA 2 cut(s) 408, 537
Tru9I TTAA 2 cut(s) 408, 537
TscAI CASTG 2 cut(s) 53, 364
TseFI GTSAC 1 cut(s) 60
TseI GCWGC 1 cut(s) 316
Tsp45I GTSAC 1 cut(s) 60
TspDTI ATGAA 5 cut(s) 86, 384, 393, 487, 523
TspGWI ACGGA 1 cut(s) 441
TspRI CASTG 2 cut(s) 53, 364
VpaK11BI GGWCC 2 cut(s) 189, 388
XapI RAATTY 3 cut(s) 216, 344, 416
XcmI CCANNNNNNNNNTGG 1 cut(s) 199
XspI CTAG 1 cut(s) 186
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.