MD10G1176000.v1.1

Photosystem II reaction center PSB28 protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0010122
Physical Location & Seq
Reverse (-)
26828054 .. 26829507
1454 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1176000.v1.1.491

Sequence Viewer

Length: 543 bp
ATGGCAACCCTGCAGTCACTTGCGTTCTCTTCTCCACTCTCACAATGTCTGCAGCAAACACGCCTTCATTCGGGCTTGTTATGTTGGGGTGTTCGTAGAAGTGCAGAATCCACATTCAATGGCAAATCTTTGCGCGTGTCTCGTCCCCAATGGGCGCCATTAAGACGGTGTACTCAAGCATCCAGGTCTATTAAAATGATGGTCAAACCAAAAATTCAGTTCATCCAAGGGACCGATGAACAGACAATACCAGATGTGAGGCTAACCAAATCAAAAGATGGTACAAATGGTGTGGCAATTTTCAAGTTTGAGCAGCCTTCTGTGTTTGACTCCTCTGGTGAGTTTGGTGACATCACTGGCTTTTACATGATTGACGAAGAAGGCGTCCTTTCATCGGTTGATGTTAACGCCAAATTTGTCAACGGAAAGCCTGCAGGAATTGAAGCAAAGTACATAATGCGAACTCCGAGAGATTGGGACAGATTCATGAGATTCATGGAACGCTATGCAAATGACAATGGCTTGAGTTTCATAAAAAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00195 ko01100 map00195 map01100
Pfam Domains
Protein Families
Psb28

Protein Analysis

181

Amino Acids

20.36

Weight (kDa)

9.63

Isoelectric Point (pI)

50.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Psb28 PF03912 72 - 177 1.5e-42 Psb28 protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 154
AccII CGCG 1 cut(s) 135
AcsI RAATTY 2 cut(s) 213, 413
AcyI GRCGYC 2 cut(s) 155, 384
AfaI GTAC 3 cut(s) 172, 283, 452
AfiI CCNNNNNNNGG 2 cut(s) 70, 394
AgsI TTSAA 3 cut(s) 118, 304, 443
AjnI CCWGG 1 cut(s) 182
Alw26I GTCTC 1 cut(s) 144
ApeKI GCWGC 2 cut(s) 52, 313
ApoI RAATTY 2 cut(s) 213, 413
ArsI GACNNNNNNTTYG 2 cut(s) 369, 401
AspLEI GCGC 2 cut(s) 135, 157
AspS9I GGNCC 1 cut(s) 231
AsuHPI GGTGA 2 cut(s) 350, 359
AvaII GGWCC 1 cut(s) 231
BanI GGYRCC 1 cut(s) 154
BbvI GCAGC 2 cut(s) 64, 325
BccI CCATC 2 cut(s) 193, 272
BciT130I CCWGG 1 cut(s) 184
BcoDI GTCTC 1 cut(s) 144
BfmI CTRYAG 3 cut(s) 11, 50, 432
BfoI RGCGCY 1 cut(s) 158
BisI GCNGC 2 cut(s) 53, 314
BlsI GCNGC 2 cut(s) 54, 315
Bme1390I CCNGG 1 cut(s) 184
Bme18I GGWCC 1 cut(s) 231
BmgT120I GGNCC 1 cut(s) 231
BmiI GGNNCC 2 cut(s) 156, 232
BmrFI CCNGG 1 cut(s) 184
BmsI GCATC 1 cut(s) 188
BpuEI CTTGAG 1 cut(s) 159
BsaHI GRCGYC 2 cut(s) 155, 384
BsaJI CCNNGG 1 cut(s) 226
Bsc4I CCNNNNNNNGG 2 cut(s) 70, 394
Bse1I ACTGG 1 cut(s) 361
BseBI CCWGG 1 cut(s) 184
BseDI CCNNGG 1 cut(s) 226
BseGI GGATG 2 cut(s) 179, 222
BseLI CCNNNNNNNGG 2 cut(s) 70, 394
BseNI ACTGG 1 cut(s) 361
BseRI GAGGAG 1 cut(s) 322
BseXI GCAGC 2 cut(s) 64, 325
BsgI GTGCAG 1 cut(s) 123
Bsh1236I CGCG 1 cut(s) 135
BshNI GGYRCC 1 cut(s) 154
BslFI GGGAC 3 cut(s) 129, 244, 491
BslI CCNNNNNNNGG 2 cut(s) 70, 394
BsmAI GTCTC 1 cut(s) 144
BsmFI GGGAC 3 cut(s) 129, 244, 491
BspFNI CGCG 1 cut(s) 135
BspHI TCATGA 1 cut(s) 486
BspLI GGNNCC 2 cut(s) 156, 232
BspMAI CTGCAG 3 cut(s) 15, 54, 436
BspT107I GGYRCC 1 cut(s) 154
BsrI ACTGG 1 cut(s) 361
BssECI CCNNGG 1 cut(s) 226
BssNI GRCGYC 2 cut(s) 155, 384
BssT1I CCWWGG 1 cut(s) 226
Bst2UI CCWGG 1 cut(s) 184
Bst4CI ACNGT 1 cut(s) 168
Bst6I CTCTTC 1 cut(s) 34
BstACI GRCGYC 2 cut(s) 155, 384
BstC8I GCNNGC 1 cut(s) 432
BstF5I GGATG 2 cut(s) 179, 222
BstFNI CGCG 1 cut(s) 135
BstH2I RGCGCY 1 cut(s) 158
BstHHI GCGC 2 cut(s) 135, 157
BstMAI GTCTC 1 cut(s) 144
BstNI CCWGG 1 cut(s) 184
BstSCI CCNGG 1 cut(s) 182
BstSFI CTRYAG 3 cut(s) 11, 50, 432
BstUI CGCG 1 cut(s) 135
BstV1I GCAGC 2 cut(s) 64, 325
BtsCI GGATG 2 cut(s) 179, 222
BtsIMutI CAGTG 1 cut(s) 354
Cac8I GCNNGC 1 cut(s) 432
CciI TCATGA 1 cut(s) 486
CfoI GCGC 2 cut(s) 135, 157
Cfr13I GGNCC 1 cut(s) 231
CseI GACGC 1 cut(s) 373
Csp6I GTAC 3 cut(s) 171, 282, 451
CspCI CAANNNNNGTGG 2 cut(s) 273, 308
CviAII CATG 3 cut(s) 367, 487, 496
CviJI RGCY 6 cut(s) 75, 262, 316, 360, 430, 522
CviKI_1 RGCY 6 cut(s) 75, 262, 316, 360, 430, 522
CviQI GTAC 3 cut(s) 171, 282, 451
DinI GGCGCC 1 cut(s) 156
Eam1104I CTCTTC 1 cut(s) 34
EarI CTCTTC 1 cut(s) 34
Eco130I CCWWGG 1 cut(s) 226
Eco47I GGWCC 1 cut(s) 231
EcoRII CCWGG 1 cut(s) 182
EcoT14I CCWWGG 1 cut(s) 226
EgeI GGCGCC 1 cut(s) 156
EheI GGCGCC 1 cut(s) 156
ErhI CCWWGG 1 cut(s) 226
FaeI CATG 3 cut(s) 370, 490, 499
FaiI YATR 7 cut(s) 82, 368, 455, 488, 497, 507, 533
FalI AAGNNNNNCTT 2 cut(s) 372, 404
FaqI GGGAC 3 cut(s) 129, 244, 491
FatI CATG 3 cut(s) 366, 486, 495
Fnu4HI GCNGC 2 cut(s) 53, 314
FokI GGATG 2 cut(s) 166, 209
Fsp4HI GCNGC 2 cut(s) 53, 314
GlaI GCGC 2 cut(s) 134, 156
GluI GCNGC 2 cut(s) 53, 314
HaeII RGCGCY 1 cut(s) 158
HgaI GACGC 1 cut(s) 373
HhaI GCGC 2 cut(s) 135, 157
Hin1I GRCGYC 2 cut(s) 155, 384
Hin1II CATG 3 cut(s) 370, 490, 499
Hin6I GCGC 2 cut(s) 133, 155
HinP1I GCGC 2 cut(s) 133, 155
HincII GTYRAC 2 cut(s) 406, 421
HindII GTYRAC 2 cut(s) 406, 421
HinfI GANTC 4 cut(s) 107, 329, 483, 492
HpaI GTTAAC 1 cut(s) 406
HphI GGTGA 2 cut(s) 350, 359
Hpy166II GTNNAC 3 cut(s) 171, 406, 421
Hpy188I TCNGA 1 cut(s) 468
Hpy188III TCNNGA 1 cut(s) 487
Hpy8I GTNNAC 3 cut(s) 171, 406, 421
HpyAV CCTTC 3 cut(s) 74, 327, 374
HpyCH4III ACNGT 1 cut(s) 168
HpyCH4V TGCA 5 cut(s) 13, 52, 104, 434, 509
Hsp92I GRCGYC 2 cut(s) 155, 384
Hsp92II CATG 3 cut(s) 370, 490, 499
HspAI GCGC 2 cut(s) 133, 155
KasI GGCGCC 1 cut(s) 154
KspAI GTTAAC 1 cut(s) 406
LpnPI CCDG 8 cut(s) 23, 169, 196, 264, 321, 342, 420, 444
Lsp1109I GCAGC 2 cut(s) 64, 325
LweI GCATC 1 cut(s) 188
MaeIII GTNAC 2 cut(s) 15, 347
MboII GAAGA 2 cut(s) 21, 389
MluCI AATT 4 cut(s) 213, 297, 413, 438
Mly113I GGCGCC 1 cut(s) 155
MlyI GAGTC 1 cut(s) 323
MnlI CCTC 2 cut(s) 252, 343
MseI TTAA 3 cut(s) 161, 192, 405
MspR9I CCNGG 1 cut(s) 184
MvaI CCWGG 1 cut(s) 184
MvnI CGCG 1 cut(s) 135
NarI GGCGCC 1 cut(s) 155
NlaIII CATG 3 cut(s) 370, 490, 499
NlaIV GGNNCC 2 cut(s) 156, 232
NmuCI GTSAC 2 cut(s) 15, 347
PagI TCATGA 1 cut(s) 486
PcsI WCGNNNNNNNCGW 1 cut(s) 381
PfeI GAWTC 3 cut(s) 107, 483, 492
PkrI GCNGC 2 cut(s) 54, 315
PleI GAGTC 1 cut(s) 323
PluTI GGCGCC 1 cut(s) 158
PpsI GAGTC 1 cut(s) 323
Psp6I CCWGG 1 cut(s) 182
PspGI CCWGG 1 cut(s) 182
PspN4I GGNNCC 2 cut(s) 156, 232
PspPI GGNCC 1 cut(s) 231
PsrI GAACNNNNNNTAC 2 cut(s) 231, 263
PstI CTGCAG 3 cut(s) 15, 54, 436
RsaI GTAC 3 cut(s) 172, 283, 452
RsaNI GTAC 3 cut(s) 171, 282, 451
SaqAI TTAA 3 cut(s) 161, 192, 405
SatI GCNGC 2 cut(s) 53, 314
Sau96I GGNCC 1 cut(s) 231
SbfI CCTGCAGG 1 cut(s) 436
SchI GAGTC 1 cut(s) 323
ScrFI CCNGG 1 cut(s) 184
SdaI CCTGCAGG 1 cut(s) 436
SetI ASST 1 cut(s) 188
SfaNI GCATC 1 cut(s) 188
SfcI CTRYAG 3 cut(s) 11, 50, 432
SfoI GGCGCC 1 cut(s) 156
SinI GGWCC 1 cut(s) 231
SmlI CTYRAG 2 cut(s) 174, 523
SmoI CTYRAG 2 cut(s) 174, 523
Sse8387I CCTGCAGG 1 cut(s) 436
Sse9I AATT 4 cut(s) 213, 297, 413, 438
SspDI GGCGCC 1 cut(s) 154
StyD4I CCNGG 1 cut(s) 182
StyI CCWWGG 1 cut(s) 226
TaaI ACNGT 1 cut(s) 168
TaqII GACCGA 1 cut(s) 248
TasI AATT 4 cut(s) 213, 297, 413, 438
TatI WGTACW 2 cut(s) 170, 450
TfiI GAWTC 3 cut(s) 107, 483, 492
Tru1I TTAA 3 cut(s) 161, 192, 405
Tru9I TTAA 3 cut(s) 161, 192, 405
TscAI CASTG 1 cut(s) 361
TseFI GTSAC 2 cut(s) 15, 347
TseI GCWGC 2 cut(s) 52, 313
Tsp45I GTSAC 2 cut(s) 15, 347
TspDTI ATGAA 7 cut(s) 56, 211, 252, 381, 475, 484, 520
TspGWI ACGGA 1 cut(s) 438
TspRI CASTG 1 cut(s) 361
VpaK11BI GGWCC 1 cut(s) 231
XapI RAATTY 2 cut(s) 213, 413
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.