Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000007

Orthogroup ID

OG0016576

Physical Location & Seq

Genomic Coordinates Reverse (-)

21189456 .. 21191767

Gene Structure 2312 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_7G00182190.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2259 bp

ATGGAGTATACCAAATCACATTTCTTTGTTTCAACACTCTTTCTTTGCTTGCTTTCTCTTCGGGCAAACACTCTCCAAACTTACATAGTTCAGCTCCACCCACAAGGTGTAACTGGCTCTTCTTTCGCATCCAAAGCCGATTGGCATCTCTCTTTTCTCGAACAAACCATGTCCTCTGTTGACGAAGATCCTTCTTCTCGCCTTCTTTACTCTTACCATTCCGCCATGGAAGGCTTTGCGGCTCAGCTGTCAGAGTCAGAGCTCGAGTCCTTGAAAATGCTGCCTGATGTGGTTGCAATTAGGCCTGACCGAATTCACCAAATTCACACCACTTACTCTTACAAGTTCTTGGGGCTCGACCCTGCTTCCAAAGAAGGCGCTTGGCACAAGTCCTCATTCGGTCGAGGAACCATCATTGGGGTGCTTGATACTGGAGTTTGGCCCGAGAGTCCAAGCTTCAATGATTATGGAATGCCGCGGGTTCCCAAGAAATGGAGGGGGATTTGCCAACAAGGGATAGACTTCAATTCCTCAAATTGCAACAGAAAACTCATTGGTGCCAGGTTCTTCACCAGAGGCCATCGTGTGGCTTCCATGCCAAACTCGCCTGACGGTGTTCAAGATTACGCATCTCCCCGTGATACCCAAGGGCATGGTACTCACACATCATCAACAGCTGGGGGTGCTTCAGTCGGAATGGCAAGTGTTTTGGGCAATGCAGCTGGTGTGGCTCGAGGGATGGCTCCCGGAGCACATGTAGCGGTGTACAAAGTCTGCTGGCTCAATGGTTGTTATAGCTCTGATATCCTTGCTGCAATGGATGTTGCAATTATAGATGGAGTAGACGTTCTCTCCCTCTCTCTAGGTGGCTTCCCCATTCCACTTTTTGATGACACCATTGCCATTGGAAGTTTTCGAGCAATGGAGCATGGAATTTCAGTTGTATGTGCAGCAGGAAACAATGGTCCAATCGAAAGCTCAGTTGCCAATGAAGCTCCTTGGATTGCCACCATCGGCGCGAGCACATTAGACCGGAGATTCCCGGCCATAGTTCAAATGGGTGATGGAAAAGTCCTTTATGGAGAATCCATGTACCCAGGAAACCGTTTGATGAGAAGTACTGGAAAAGAGGGGTCTCTCCCAAGAACAAAAGTACGCGGCAAGATAGTGGTCTGTGACCGTGGTGTCAATGGTAGGGCGGAGAAAGGTGAAGTGGTAAAAGAAGCTGGAGGAGCTGGAATGATTTTGGCCAACACAGAGGTAAACCTGGAGGAGGACTCTGTTGATGTCCATGTCTTGCCCGCGACTCTGATTGGCTACAGAGAGTCCATTCACTTGAAGGCTTACATAAACTCTACCAGGAGACCAATGGCTAGAATTGTGTTTGAAGGCACAGTTATAGGGAAGTCCAGAGCACCAGCAGTAGCTCAGTTCTCAGCTAGAGGACCCAGTTTTTCGAACCCTTCAATCCTCAAACCTGATGTGATTGCTCCGGGAGTCAACATCATTGCTGCTTGGCCTCAAAACATGGGTCCCACCGGCCTTCCAGAAGATTCTAGGAGAGTGAATTTCACTGTCATGTCAGGGACTTCAATGGCATGTCCTCATGCCAGTGGAATTGCTGCACTAGTACGCACAGCTCATCCAAAATGGAGCCCCGCAGCTATCAAATCTGCTATTATGACGACTGCTGATGTAACTGATCATTCAGGGAATCCGATAATGGACGGAGGCAAACCAGCCGGAGTTTTTGCAATTGGTGCTGGACATGTAAACCCTGTGAGAGCTGTTGATCCGGGGTTGATCTACGACATCAGGCCGGAGGAGTATGTCACTCATTTGTGCACTCTTGGATACAAGAAATCGGATATCTTAACAATCACTCACAGAAATGTTAGCTGCCTTGAAATTTTGAAGATGAATAGGGGTTTCAGCCTCAATTACCCCTCCATTTCAGTAACTTTCAAACACGGCATGACAAGTAAAATGATCAGACGACGAGTAACAAACGTAGGGAATCCGCATTCCACTTACTTGCTGGAAGTAATGGCACCTAAGGAAGTTAGAGTGAAAGTTAAGCCTCAGAGGTTAATTTTCACAGGCATCAACCAGAGTTTGAGTTATAGGGTATGGTTTATATCGAGGAAAAGAATCAGAAAAGATAAGATGAGCTTCGCACAAGGACATTTAGCATGGGTGAATTCTAACAATAGTAGCTTTCACAAGGTCAAAAGTCCCATCTCTGTGACTTGGAAGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004175 GO:0004252 GO:0005575 GO:0005623 GO:0005886 GO:0006508 GO:0006807 GO:0007275 GO:0008150 GO:0008152 GO:0008233 GO:0008236 GO:0009653 GO:0009791 GO:0009888 GO:0009897 GO:0009986 GO:0010016 GO:0010103 GO:0010374 GO:0016020 GO:0016787 GO:0017171 GO:0019538 GO:0032501 GO:0032502 GO:0042127 GO:0043170 GO:0044238 GO:0044425 GO:0044459 GO:0044464 GO:0048367 GO:0048731 GO:0048856 GO:0050789 GO:0050794 GO:0065007 GO:0070011 GO:0071704 GO:0071944 GO:0090558 GO:0090626 GO:0090698 GO:0098552 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Inhibitor_I9 PA Peptidase_S8

Protein Analysis

752

Amino Acids

81.54

Weight (kDa)

9.42

Isoelectric Point (pI)

35.74

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Inhibitor_I9	PF05922	27 - 109	1e-17	Peptidase inhibitor I9
Peptidase_S8	PF00082	134 - 568	5.2e-48	Subtilase family
PA	PF02225	381 - 442	4.8e-12	PA domain
fn3_6	PF17766	646 - 749	1.2e-23	Fibronectin type-III domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0016576)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G04110
fragaria_vesca	FvH4_2g20460
malus_domestica	MD05G1188400.v1.1 MD10G1175900.v1.1
prunus_persica	Prupe.8G214900_v2.0.a1
pyrus_communis	pycom10g15150
rosa_chinensis	RchiOBHm_Chr6g0286551
rosa_laevigata	RLG00000012558
rosa_multiflora	Rmu_sc0004628.1_g000013
rosa_roxburghii	Rroxscaffold_7G00182190
rosa_rugosa	Rorug06G0181100
rosa_samantha	Rh6AG293900 Rh6BG297000
rosa_wichuraiana	Rw6G025300

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	1184
AccB1I	GGYRCC	2 cut(s)	557, 2050
AccB7I	CCANNNNNTGG	2 cut(s)	492, 586
AccI	GTMKAC	2 cut(s)	8, 843
AccII	CGCG	4 cut(s)	478, 1019, 1158, 1304
AclWI	GGATC	2 cut(s)	182, 1787
AcoI	YGGCCR	2 cut(s)	1044, 1248
AcsI	RAATTY	6 cut(s)	312, 321, 933, 1567, 1908, 2200
AcuI	CTGAAG	1 cut(s)	672
AdeI	CACNNNGTG	1 cut(s)	107
AfaI	GTAC	6 cut(s)	658, 767, 1094, 1120, 1155, 1632
AfiI	CCNNNNNNNGG	4 cut(s)	417, 492, 586, 1273
AflIII	ACRYGT	2 cut(s)	754, 1768
AhlI	ACTAGT	1 cut(s)	1627
AjnI	CCWGG	4 cut(s)	560, 1096, 1266, 1358
AjuI	GAANNNNNNNTTGG	1 cut(s)	37
AloI	GAACNNNNNNTCC	2 cut(s)	831, 863
Alw21I	GWGCWC	5 cut(s)	264, 754, 1025, 1417, 1847
Alw26I	GTCTC	2 cut(s)	1140, 1357
Alw44I	GTGCAC	1 cut(s)	1843
AlwI	GGATC	2 cut(s)	182, 1787
Ama87I	CYCGRG	3 cut(s)	263, 443, 732
AoxI	GGCC	8 cut(s)	302, 440, 577, 1044, 1248, 1517, 1540, 1817
ApaLI	GTGCAC	1 cut(s)	1843
ApeKI	GCWGC	8 cut(s)	280, 719, 812, 950, 1511, 1622, 1661, 1899
ApoI	RAATTY	6 cut(s)	312, 321, 933, 1567, 1908, 2200
ArsI	GACNNNNNNTTYG	2 cut(s)	1516, 1548
AspLEI	GCGC	2 cut(s)	380, 1019
AspS9I	GGNCC	4 cut(s)	441, 965, 1445, 1532
AsuC2I	CCSGG	4 cut(s)	747, 1043, 1494, 1797
AsuHPI	GGTGA	5 cut(s)	308, 562, 1073, 1220, 2209
AsuII	TTCGAA	1 cut(s)	1457
AvaI	CYCGRG	3 cut(s)	263, 443, 732
AvaII	GGWCC	3 cut(s)	965, 1445, 1532
AxyI	CCTNAGG	1 cut(s)	2055
BaeGI	GKGCMC	1 cut(s)	1847
BaeI	ACNNNNGTAYC	2 cut(s)	420, 453
BalI	TGGCCA	1 cut(s)	1250
BanI	GGYRCC	2 cut(s)	557, 2050
BanII	GRGCYC	3 cut(s)	264, 357, 1658
Bbv12I	GWGCWC	5 cut(s)	264, 754, 1025, 1417, 1847
BbvI	GCAGC	8 cut(s)	267, 731, 799, 962, 1498, 1609, 1673, 1886
BccI	CCATC	7 cut(s)	419, 588, 733, 830, 1019, 1058, 2246
BceAI	ACGGC	1 cut(s)	1987
BciT130I	CCWGG	4 cut(s)	562, 1098, 1268, 1360
BciVI	GTATCC	1 cut(s)	1847
BclI	TGATCA	2 cut(s)	1702, 1989
BcnI	CCSGG	4 cut(s)	747, 1043, 1494, 1797
BcoDI	GTCTC	2 cut(s)	1140, 1357
BcuI	ACTAGT	1 cut(s)	1627
BfaI	CTAG	5 cut(s)	863, 1374, 1440, 1557, 1628
BfmI	CTRYAG	1 cut(s)	1318
BfoI	RGCGCY	1 cut(s)	381
BfuI	GTATCC	1 cut(s)	1847
BlpI	GCTNAGC	1 cut(s)	243
BmcAI	AGTACT	1 cut(s)	1120
Bme1390I	CCNGG	8 cut(s)	562, 747, 1043, 1098, 1268, 1360, 1494, 1797
Bme18I	GGWCC	3 cut(s)	965, 1445, 1532
BmeT110I	CYCGRG	3 cut(s)	263, 443, 732
BmgT120I	GGNCC	4 cut(s)	441, 965, 1445, 1532
BmiI	GGNNCC	9 cut(s)	409, 483, 559, 744, 1447, 1533, 1534, 1655, 2052
BmrFI	CCNGG	8 cut(s)	562, 747, 1043, 1098, 1268, 1360, 1494, 1797
BmrI	ACTGGG	1 cut(s)	1443
BmsI	GCATC	4 cut(s)	137, 154, 638, 2112
BmuI	ACTGGG	1 cut(s)	1443
BplI	GAGNNNNNCTC	2 cut(s)	1262, 1294
BpmI	CTGGAG	3 cut(s)	453, 1248, 1289
Bpu1102I	GCTNAGC	1 cut(s)	243
Bpu14I	TTCGAA	1 cut(s)	1457
BpuMI	CCSGG	4 cut(s)	747, 1043, 1494, 1797
BsaBI	GATNNNNATC	1 cut(s)	144
BsaI	GGTCTC	2 cut(s)	1140, 1357
BsaJI	CCNNGG	7 cut(s)	225, 476, 646, 998, 1096, 1180, 1796
BsaWI	WCCGGW	1 cut(s)	1032
BsaXI	ACNNNNNCTCC	6 cut(s)	741, 771, 831, 836, 861, 866
Bsc4I	CCNNNNNNNGG	4 cut(s)	417, 492, 586, 1273
Bse118I	RCCGGY	1 cut(s)	1538
Bse1I	ACTGG	5 cut(s)	118, 436, 1126, 1449, 1611
Bse21I	CCTNAGG	1 cut(s)	2055
Bse3DI	GCAATG	5 cut(s)	721, 822, 897, 927, 1506
Bse8I	GATNNNNATC	1 cut(s)	144
BseBI	CCWGG	4 cut(s)	562, 1098, 1268, 1360
BseDI	CCNNGG	7 cut(s)	225, 476, 646, 998, 1096, 1180, 1796
BseGI	GGATG	4 cut(s)	128, 744, 826, 1642
BseJI	GATNNNNATC	1 cut(s)	144
BseLI	CCNNNNNNNGG	4 cut(s)	417, 492, 586, 1273
BseMI	GCAATG	5 cut(s)	721, 822, 897, 927, 1506
BseMII	CTCAG	5 cut(s)	257, 993, 1442, 1449, 2096
BseNI	ACTGG	5 cut(s)	118, 436, 1126, 1449, 1611
BseRI	GAGGAG	3 cut(s)	1245, 1286, 1838
BseSI	GKGCMC	1 cut(s)	1847
BseXI	GCAGC	8 cut(s)	267, 731, 799, 962, 1498, 1609, 1673, 1886
BseYI	CCCAGC	1 cut(s)	677
BsgI	GTGCAG	2 cut(s)	969, 1608
Bsh1236I	CGCG	4 cut(s)	478, 1019, 1158, 1304
Bsh1285I	CGRYCG	1 cut(s)	403
BshFI	GGCC	8 cut(s)	304, 442, 579, 1046, 1250, 1519, 1542, 1819
BshNI	GGYRCC	2 cut(s)	557, 2050
BsiEI	CGRYCG	1 cut(s)	403
BsiHKAI	GWGCWC	5 cut(s)	264, 754, 1025, 1417, 1847
BsiHKCI	CYCGRG	3 cut(s)	263, 443, 732
BsiSI	CCGG	8 cut(s)	747, 1033, 1043, 1493, 1539, 1743, 1796, 1820
BslFI	GGGAC	3 cut(s)	1518, 1600, 2220
BslI	CCNNNNNNNGG	4 cut(s)	417, 492, 586, 1273
BsmAI	GTCTC	2 cut(s)	1140, 1357
BsmFI	GGGAC	3 cut(s)	1518, 1600, 2220
BsmI	GAATGC	2 cut(s)	477, 2023
BsnI	GGCC	8 cut(s)	304, 442, 579, 1046, 1250, 1519, 1542, 1819
Bso31I	GGTCTC	2 cut(s)	1140, 1357
BsoBI	CYCGRG	3 cut(s)	263, 443, 732
Bsp119I	TTCGAA	1 cut(s)	1457
Bsp1286I	GDGCHC	7 cut(s)	264, 357, 754, 1025, 1417, 1658, 1847
Bsp1407I	TGTACA	1 cut(s)	765
Bsp143I	GATC	5 cut(s)	187, 1702, 1792, 1803, 1989
Bsp1720I	GCTNAGC	1 cut(s)	243
Bsp19I	CCATGG	1 cut(s)	225
BspANI	GGCC	8 cut(s)	304, 442, 579, 1046, 1250, 1519, 1542, 1819
BspCNI	CTCAG	5 cut(s)	256, 992, 1441, 1448, 2095
BspFNI	CGCG	4 cut(s)	478, 1019, 1158, 1304
BspLI	GGNNCC	9 cut(s)	409, 483, 559, 744, 1447, 1533, 1534, 1655, 2052
BspPI	GGATC	2 cut(s)	182, 1787
BspQI	GCTCTTC	1 cut(s)	124
BspT104I	TTCGAA	1 cut(s)	1457
BspT107I	GGYRCC	2 cut(s)	557, 2050
BspTNI	GGTCTC	2 cut(s)	1140, 1357
BsrDI	GCAATG	5 cut(s)	721, 822, 897, 927, 1506
BsrFI	RCCGGY	1 cut(s)	1538
BsrGI	TGTACA	1 cut(s)	765
BsrI	ACTGG	5 cut(s)	118, 436, 1126, 1449, 1611
BssAI	RCCGGY	1 cut(s)	1538
BssECI	CCNNGG	7 cut(s)	225, 476, 646, 998, 1096, 1180, 1796
BssMI	GATC	5 cut(s)	187, 1702, 1792, 1803, 1989
BssNAI	GTATAC	1 cut(s)	9
BssT1I	CCWWGG	3 cut(s)	225, 646, 998
Bst1107I	GTATAC	1 cut(s)	9
Bst2UI	CCWGG	4 cut(s)	562, 1098, 1268, 1360
Bst4CI	ACNGT	5 cut(s)	614, 1106, 1181, 1396, 1576
Bst6I	CTCTTC	2 cut(s)	63, 124
BstAPI	GCANNNNNTGC	1 cut(s)	1760
BstAUI	TGTACA	1 cut(s)	765
BstBI	TTCGAA	1 cut(s)	1457
BstC8I	GCNNGC	4 cut(s)	50, 779, 1021, 1302
BstDEI	CTNAG	6 cut(s)	243, 979, 1428, 1435, 2055, 2082
BstDSI	CCRYGG	3 cut(s)	225, 476, 1180
BstENI	CCTNNNNNAGG	1 cut(s)	1271
BstF5I	GGATG	4 cut(s)	128, 744, 826, 1642
BstFNI	CGCG	4 cut(s)	478, 1019, 1158, 1304
BstH2I	RGCGCY	1 cut(s)	381
BstHHI	GCGC	2 cut(s)	380, 1019
BstKTI	GATC	5 cut(s)	190, 1705, 1795, 1806, 1992
BstMAI	GTCTC	2 cut(s)	1140, 1357
BstMBI	GATC	5 cut(s)	187, 1702, 1792, 1803, 1989
BstMCI	CGRYCG	1 cut(s)	403
BstMWI	GCNNNNNNNGC	9 cut(s)	134, 604, 683, 728, 749, 758, 992, 1232, 1760
BstNI	CCWGG	4 cut(s)	562, 1098, 1268, 1360
BstNSI	RCATGY	3 cut(s)	758, 1602, 1772
BstSCI	CCNGG	8 cut(s)	560, 745, 1041, 1096, 1266, 1358, 1492, 1795
BstSFI	CTRYAG	1 cut(s)	1318
BstSLI	GKGCMC	1 cut(s)	1847
BstUI	CGCG	4 cut(s)	478, 1019, 1158, 1304
BstV1I	GCAGC	8 cut(s)	267, 731, 799, 962, 1498, 1609, 1673, 1886
BstX2I	RGATCY	1 cut(s)	187
BstXI	CCANNNNNNTGG	1 cut(s)	653
BstYI	RGATCY	1 cut(s)	187
BstZ17I	GTATAC	1 cut(s)	9
Bsu36I	CCTNAGG	1 cut(s)	2055
BsuI	GTATCC	1 cut(s)	1847
BsuRI	GGCC	8 cut(s)	304, 442, 579, 1046, 1250, 1519, 1542, 1819
BtgI	CCRYGG	3 cut(s)	225, 476, 1180
BtsCI	GGATG	4 cut(s)	128, 744, 826, 1642
BtsIMutI	CAGTG	2 cut(s)	1572, 1618
Cac8I	GCNNGC	4 cut(s)	50, 779, 1021, 1302
CfoI	GCGC	2 cut(s)	380, 1019
Cfr10I	RCCGGY	1 cut(s)	1538
Cfr13I	GGNCC	4 cut(s)	441, 965, 1445, 1532
Cfr42I	CCGCGG	1 cut(s)	479
Csp6I	GTAC	6 cut(s)	657, 766, 1093, 1119, 1154, 1631
CviQI	GTAC	6 cut(s)	657, 766, 1093, 1119, 1154, 1631
DdeI	CTNAG	6 cut(s)	243, 979, 1428, 1435, 2055, 2082
DpnI	GATC	5 cut(s)	189, 1704, 1794, 1805, 1991
DpnII	GATC	5 cut(s)	187, 1702, 1792, 1803, 1989
DraIII	CACNNNGTG	1 cut(s)	107
DrdI	GACNNNNNNGTC	1 cut(s)	1184
DseDI	GACNNNNNNGTC	1 cut(s)	1184
EaeI	YGGCCR	2 cut(s)	1044, 1248
Eam1104I	CTCTTC	2 cut(s)	63, 124
EarI	CTCTTC	2 cut(s)	63, 124
EciI	GGCGGA	2 cut(s)	211, 1214
Ecl136II	GAGCTC	1 cut(s)	262
Eco130I	CCWWGG	3 cut(s)	225, 646, 998
Eco147I	AGGCCT	1 cut(s)	304
Eco24I	GRGCYC	3 cut(s)	264, 357, 1658
Eco31I	GGTCTC	2 cut(s)	1140, 1357
Eco32I	GATATC	2 cut(s)	805, 1870
Eco47I	GGWCC	3 cut(s)	965, 1445, 1532
Eco53kI	GAGCTC	1 cut(s)	262
Eco57I	CTGAAG	1 cut(s)	672
Eco81I	CCTNAGG	1 cut(s)	2055
Eco88I	CYCGRG	3 cut(s)	263, 443, 732
EcoICRI	GAGCTC	1 cut(s)	262
EcoNI	CCTNNNNNAGG	1 cut(s)	1271
EcoO109I	RGGNCCY	2 cut(s)	1445, 1532
EcoRI	GAATTC	2 cut(s)	312, 2200
EcoRII	CCWGG	4 cut(s)	560, 1096, 1266, 1358
EcoRV	GATATC	2 cut(s)	805, 1870
EcoT14I	CCWWGG	3 cut(s)	225, 646, 998
EcoT38I	GRGCYC	3 cut(s)	264, 357, 1658
ErhI	CCWWGG	3 cut(s)	225, 646, 998
FalI	AAGNNNNNCTT	2 cut(s)	2156, 2188
FaqI	GGGAC	3 cut(s)	1518, 1600, 2220
FauI	CCCGC	3 cut(s)	471, 1309, 1666
FbaI	TGATCA	2 cut(s)	1702, 1989
FblI	GTMKAC	2 cut(s)	8, 843
FokI	GGATG	4 cut(s)	115, 751, 833, 1629
FriOI	GRGCYC	3 cut(s)	264, 357, 1658
FspBI	CTAG	5 cut(s)	863, 1374, 1440, 1557, 1628
GlaI	GCGC	2 cut(s)	379, 1018
GsaI	CCCAGC	1 cut(s)	681
GsuI	CTGGAG	3 cut(s)	453, 1248, 1289
HaeII	RGCGCY	1 cut(s)	381
HaeIII	GGCC	8 cut(s)	304, 442, 579, 1046, 1250, 1519, 1542, 1819
HapII	CCGG	8 cut(s)	747, 1033, 1043, 1493, 1539, 1743, 1796, 1820
HhaI	GCGC	2 cut(s)	380, 1019
Hin6I	GCGC	2 cut(s)	378, 1017
HinP1I	GCGC	2 cut(s)	378, 1017
HincII	GTYRAC	2 cut(s)	181, 1501
HindII	GTYRAC	2 cut(s)	181, 1501
HindIII	AAGCTT	1 cut(s)	454
HpaII	CCGG	8 cut(s)	747, 1033, 1043, 1493, 1539, 1743, 1796, 1820
HphI	GGTGA	5 cut(s)	308, 562, 1073, 1220, 2209
Hpy166II	GTNNAC	8 cut(s)	9, 181, 766, 844, 1264, 1501, 1774, 1845
Hpy188III	TCNNGA	4 cut(s)	158, 620, 1410, 1547
Hpy8I	GTNNAC	8 cut(s)	9, 181, 766, 844, 1264, 1501, 1774, 1845
Hpy99I	CGWCG	1 cut(s)	2001
HpyAV	CCTTC	8 cut(s)	201, 212, 224, 368, 1333, 1382, 1473, 1553
HpyCH4III	ACNGT	5 cut(s)	614, 1106, 1181, 1396, 1576
HpyCH4IV	ACGT	2 cut(s)	846, 2010
HpyCH4V	TGCA	9 cut(s)	296, 540, 719, 815, 827, 950, 1625, 1754, 1845
HpyF10VI	GCNNNNNNNGC	9 cut(s)	134, 604, 683, 728, 749, 758, 992, 1232, 1760
HpyF3I	CTNAG	6 cut(s)	243, 979, 1428, 1435, 2055, 2082
HpySE526I	ACGT	2 cut(s)	846, 2010
HspAI	GCGC	2 cut(s)	378, 1017
KflI	GGGWCCC	1 cut(s)	1532
Ksp22I	TGATCA	2 cut(s)	1702, 1989
KspI	CCGCGG	1 cut(s)	479
Kzo9I	GATC	5 cut(s)	187, 1702, 1792, 1803, 1989
LguI	GCTCTTC	1 cut(s)	124
LmnI	GCTCC	8 cut(s)	99, 748, 749, 925, 1000, 1232, 1495, 1653
Lsp1109I	GCAGC	8 cut(s)	267, 731, 799, 962, 1498, 1609, 1673, 1886
LweI	GCATC	4 cut(s)	137, 154, 638, 2112
MaeI	CTAG	5 cut(s)	863, 1374, 1440, 1557, 1628
MaeII	ACGT	2 cut(s)	846, 2010
MaeIII	GTNAC	7 cut(s)	109, 1175, 1696, 1831, 1957, 2002, 2245
MalI	GATC	5 cut(s)	189, 1704, 1794, 1805, 1991
MboI	GATC	5 cut(s)	187, 1702, 1792, 1803, 1989
MboII	GAAGA	7 cut(s)	50, 111, 186, 197, 559, 1562, 1927
MfeI	CAATTG	1 cut(s)	1755
MflI	RGATCY	1 cut(s)	187
MhlI	GDGCHC	7 cut(s)	264, 357, 754, 1025, 1417, 1658, 1847
MlsI	TGGCCA	1 cut(s)	1250
MluNI	TGGCCA	1 cut(s)	1250
MlyI	GAGTC	7 cut(s)	263, 275, 457, 1271, 1300, 1334, 1506
MmeI	TCCRAC	1 cut(s)	673
Mox20I	TGGCCA	1 cut(s)	1250
MscI	TGGCCA	1 cut(s)	1250
MseI	TTAA	3 cut(s)	1874, 2076, 2090
MslI	CAYNNNNRTG	5 cut(s)	419, 1577, 1611, 1890, 2243
Msp20I	TGGCCA	1 cut(s)	1250
MspA1I	CMGCKG	4 cut(s)	247, 478, 677, 722
MspI	CCGG	8 cut(s)	747, 1033, 1043, 1493, 1539, 1743, 1796, 1820
MspR9I	CCNGG	8 cut(s)	562, 747, 1043, 1098, 1268, 1360, 1494, 1797
MunI	CAATTG	1 cut(s)	1755
Mva1269I	GAATGC	2 cut(s)	477, 2023
MvaI	CCWGG	4 cut(s)	562, 1098, 1268, 1360
MvnI	CGCG	4 cut(s)	478, 1019, 1158, 1304
MwoI	GCNNNNNNNGC	9 cut(s)	134, 604, 683, 728, 749, 758, 992, 1232, 1760
NciI	CCSGG	4 cut(s)	747, 1043, 1494, 1797
NcoI	CCATGG	1 cut(s)	225
NdeII	GATC	5 cut(s)	187, 1702, 1792, 1803, 1989
NlaIV	GGNNCC	9 cut(s)	409, 483, 559, 744, 1447, 1533, 1534, 1655, 2052
NmuCI	GTSAC	3 cut(s)	1175, 1831, 2245
NspI	RCATGY	3 cut(s)	758, 1602, 1772
NspV	TTCGAA	1 cut(s)	1457
PaeR7I	CTCGAG	2 cut(s)	263, 732
PceI	AGGCCT	1 cut(s)	304
PciI	ACATGT	2 cut(s)	754, 1768
PciSI	GCTCTTC	1 cut(s)	124
PctI	GAATGC	2 cut(s)	477, 2023
PfeI	GAWTC	6 cut(s)	1038, 1085, 1553, 1714, 2017, 2151
PflMI	CCANNNNNTGG	2 cut(s)	492, 586
PfoI	TCCNGGA	2 cut(s)	745, 1492
PleI	GAGTC	7 cut(s)	262, 274, 456, 1271, 1300, 1333, 1505
PpsI	GAGTC	7 cut(s)	262, 274, 456, 1271, 1300, 1333, 1505
PpuMI	RGGWCCY	2 cut(s)	1445, 1532
PscI	ACATGT	2 cut(s)	754, 1768
Psp124BI	GAGCTC	1 cut(s)	264
Psp5II	RGGWCCY	2 cut(s)	1445, 1532
Psp6I	CCWGG	4 cut(s)	560, 1096, 1266, 1358
PspFI	CCCAGC	1 cut(s)	677
PspGI	CCWGG	4 cut(s)	560, 1096, 1266, 1358
PspN4I	GGNNCC	9 cut(s)	409, 483, 559, 744, 1447, 1533, 1534, 1655, 2052
PspPI	GGNCC	4 cut(s)	441, 965, 1445, 1532
PspPPI	RGGWCCY	2 cut(s)	1445, 1532
PspXI	VCTCGAGB	2 cut(s)	263, 732
PsuI	RGATCY	1 cut(s)	187
PvuII	CAGCTG	3 cut(s)	247, 677, 722
RsaI	GTAC	6 cut(s)	658, 767, 1094, 1120, 1155, 1632
RsaNI	GTAC	6 cut(s)	657, 766, 1093, 1119, 1154, 1631
RseI	CAYNNNNRTG	5 cut(s)	419, 1577, 1611, 1890, 2243
SacI	GAGCTC	1 cut(s)	264
SacII	CCGCGG	1 cut(s)	479
SapI	GCTCTTC	1 cut(s)	124
SaqAI	TTAA	3 cut(s)	1874, 2076, 2090
Sau3AI	GATC	5 cut(s)	187, 1702, 1792, 1803, 1989
Sau96I	GGNCC	4 cut(s)	441, 965, 1445, 1532
ScaI	AGTACT	1 cut(s)	1120
SchI	GAGTC	7 cut(s)	263, 275, 457, 1271, 1300, 1334, 1506
ScrFI	CCNGG	8 cut(s)	562, 747, 1043, 1098, 1268, 1360, 1494, 1797
SduI	GDGCHC	7 cut(s)	264, 357, 754, 1025, 1417, 1658, 1847
SfaNI	GCATC	4 cut(s)	137, 154, 638, 2112
SfcI	CTRYAG	1 cut(s)	1318
Sfr274I	CTCGAG	2 cut(s)	263, 732
Sfr303I	CCGCGG	1 cut(s)	479
SfuI	TTCGAA	1 cut(s)	1457
SgrBI	CCGCGG	1 cut(s)	479
SinI	GGWCC	3 cut(s)	965, 1445, 1532
SlaI	CTCGAG	2 cut(s)	263, 732
SmiMI	CAYNNNNRTG	5 cut(s)	419, 1577, 1611, 1890, 2243
SmlI	CTYRAG	2 cut(s)	263, 732
SmoI	CTYRAG	2 cut(s)	263, 732
SpeI	ACTAGT	1 cut(s)	1627
SseBI	AGGCCT	1 cut(s)	304
SspMI	CTAG	5 cut(s)	863, 1374, 1440, 1557, 1628
SstI	GAGCTC	1 cut(s)	264
StuI	AGGCCT	1 cut(s)	304
StyD4I	CCNGG	8 cut(s)	560, 745, 1041, 1096, 1266, 1358, 1492, 1795
StyI	CCWWGG	3 cut(s)	225, 646, 998
TaaI	ACNGT	5 cut(s)	614, 1106, 1181, 1396, 1576
TaiI	ACGT	2 cut(s)	849, 2013
TaqI	TCGA	9 cut(s)	159, 264, 357, 403, 733, 916, 972, 1457, 2141
TaqII	GACCGA	2 cut(s)	324, 389
TatI	WGTACW	2 cut(s)	765, 1118
TauI	GCSGC	3 cut(s)	242, 478, 1161
TfiI	GAWTC	6 cut(s)	1038, 1085, 1553, 1714, 2017, 2151
Tru1I	TTAA	3 cut(s)	1874, 2076, 2090
Tru9I	TTAA	3 cut(s)	1874, 2076, 2090
TscAI	CASTG	2 cut(s)	1579, 1618
TseFI	GTSAC	3 cut(s)	1175, 1831, 2245
TseI	GCWGC	8 cut(s)	280, 719, 812, 950, 1511, 1622, 1661, 1899
Tsp45I	GTSAC	3 cut(s)	1175, 1831, 2245
TspDTI	ATGAA	2 cut(s)	1005, 1934
TspGWI	ACGGA	1 cut(s)	1743
TspRI	CASTG	2 cut(s)	1579, 1618
Van91I	CCANNNNNTGG	2 cut(s)	492, 586
VneI	GTGCAC	1 cut(s)	1843
VpaK11BI	GGWCC	3 cut(s)	965, 1445, 1532
XagI	CCTNNNNNAGG	1 cut(s)	1271
XapI	RAATTY	6 cut(s)	312, 321, 933, 1567, 1908, 2200
XceI	RCATGY	3 cut(s)	758, 1602, 1772
XcmI	CCANNNNNNNNNTGG	3 cut(s)	1054, 1366, 2035
XhoI	CTCGAG	2 cut(s)	263, 732
XmiI	GTMKAC	2 cut(s)	8, 843
XspI	CTAG	5 cut(s)	863, 1374, 1440, 1557, 1628
ZrmI	AGTACT	1 cut(s)	1120

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.