Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr05

Orthogroup ID

OG0010207

Physical Location & Seq

Genomic Coordinates Forward (+)

32087850 .. 32092147

Gene Structure 4298 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD05G1193100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1269 bp

ATGGTGAGAGGATTACTGAAGCAGCTGACCTCCCGCTCTCTCTCCGTCGCCGGAAAATGGCAGCAGCAACAGCTCCGCCGCCTCAACATCCACGAATATCAGGGGGCTGAATTGATGAGCAAATACGGGGTCAATGTCCCGAAAGGTGTCGCAGCTGGTTCCGTCGATGAAGTCAAAAAGGCAATTCAGGATGCATTTCCCAAGGAAAGTGAGTTGGTGGTTAAAAGCCAAGTCTTGGCTGGTGGAAGAGGCTTGGGAACTTTTAAAAATGGTCTCAAGGGTGGTGTTCACATTGTCCCGACTAACCAAGTTGAAGATATCGCTGGCAAGATGCTTGGGCAAATTCTTGTTACCAAGCAAACAGGTCCTCAGGGAAAAATTGTCAGCAAGGTTTATTTGTGCCAAAAGTTGTCACTTGTCAATGAGATGTACTTTTCTATCATTCTGGATCGCAAATCTGCTGGCCCACTTATAATTGCTTGTGCAAAGGGTGGAACCAGCATTGAAGACCTTGCTGAGAAATTCCCCGATCAGATAATTAAGGTACCTATCGATGTTTTCACCGGAATTACAGATGAAGATGCTGCAAAGGTTGTTGATGGTTTGGCTCCAAAAGGAGCTGATAGGAATAGTTCAATCGATCAAGTGAAGAAATTATATAAGCTATTCAGTGAGACTGACTGTACACTGCTGGAAATCAATCCACTTGCAGAAACTGCTGATAACCAACTAGTAGCTGCTGATGCCAAGTTGAACTTTGACGACAATGCTGCTTATCGTCAGAAAGAGATATTTGCTCTTCGCGATCCATCGCAGGAGGATCCCCGAGAGGTGGCTGCTGCCAAAGCAGATTTGAATTATATTGGCTTAGATGGGGAGATTGGTTGCATGGTGAATGGTGCAGGATTAGCTATGGCTACTATGGATATCATTAAGTTACACGGGGGAACTCCTGCCAATTTTCTAGATGTTGGCGGAAATGCTTCTGAAAACCAGGTTGTTGAGGCGTTTAAGATATTGACTTCTGATGAGAAGGTGAAAGCCATTTTGGTCAATATATTTGGTGGAATAATGAAATGTGATGTGATTGCAAGTGGAATTGTCAATGCTGCTAAACTGGTTAAACTAGAAGTGCCGGTGATTGTTCGCCTTGAAGGTACCAATGTTGACCAGGGAAAGAGGATTCTCAAGGAAAGTGGAATGGCGCTAATTACAGCAGAAGATCTGGACGATGCCGCTGATAAAGCGGTTAAAGCGCTGAAGAAATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000166 GO:0003674 GO:0003824 GO:0004774 GO:0004775 GO:0005488 GO:0005507 GO:0005524 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0006082 GO:0006091 GO:0006099 GO:0006101 GO:0006104 GO:0006105 GO:0006139 GO:0006163 GO:0006637 GO:0006725 GO:0006732 GO:0006753 GO:0006790 GO:0006793 GO:0006796 GO:0006807 GO:0008144 GO:0008150 GO:0008152 GO:0009060 GO:0009117 GO:0009150 GO:0009259 GO:0009987 GO:0010035 GO:0010038 GO:0015980 GO:0016874 GO:0016877 GO:0016878 GO:0016999 GO:0017076 GO:0017144 GO:0019637 GO:0019693 GO:0019752 GO:0030554 GO:0032553 GO:0032555 GO:0032559 GO:0033865 GO:0033875 GO:0034032 GO:0034641 GO:0035383 GO:0035639 GO:0036094 GO:0042221 GO:0043167 GO:0043168 GO:0043169 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0043603 GO:0043648 GO:0044237 GO:0044238 GO:0044281 GO:0044424 GO:0044444 GO:0044464 GO:0045333 GO:0046483 GO:0046686 GO:0046872 GO:0046914 GO:0050896 GO:0051186 GO:0055086 GO:0055114 GO:0071704 GO:0072350 GO:0072521 GO:0097159 GO:0097367 GO:1901135 GO:1901265 GO:1901360 GO:1901363 GO:1901564

KEGG Pathways

Metabolic & Signaling

ko00020 ko00640 ko01100 ko01110 ko01120 ko01130 ko01200 map00020 map00640 map01100 map01110 map01120 map01130 map01200

Pfam Domains

Protein Families

ATP-grasp_2 Ligase_CoA

Protein Analysis

423

Amino Acids

45.19

Weight (kDa)

6.09

Isoelectric Point (pI)

27.46

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
ATP-grasp_2	PF08442	29 - 237	1e-59	ATP-grasp domain
Citrate_synth_N	PF24948	30 - 262	4.3e-12	ATP-citrate synthase ATP-grasp domain
Ligase_CoA	PF00549	297 - 417	3.2e-36	CoA-ligase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0010207)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G20420
fragaria_vesca	FvH4_2g20990 FvH4_2g20990 FvH4_2g20990
malus_domestica	MD05G1193100.v1.1 MD10G1179600.v1.1
prunus_persica	Prupe.8G219400_v2.0.a1 Prupe.8G219400_v2.0.a1
pyrus_communis	pycom05g18000 pycom10g15570
rosa_chinensis	RchiOBHm_Chr6g0287371
rosa_laevigata	RLG00000012494
rosa_multiflora	Rmu_sc0007029.1_g000025
rosa_roxburghii	Rroxscaffold_7G00181490
rosa_rugosa	Rorug06G0187700.1
rosa_samantha	Rh6AG298900 Rh6BG304500 Rh6CG305200 Rh6CG313000 Rh6DG296300
rosa_wichuraiana	Rw6G025830

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	473
Acc65I	GGTACC	2 cut(s)	544, 1157
AccB1I	GGYRCC	2 cut(s)	544, 1157
AccB7I	CCANNNNNTGG	1 cut(s)	235
AccBSI	CCGCTC	1 cut(s)	36
AccII	CGCG	1 cut(s)	804
AciI	CCGC	6 cut(s)	34, 76, 79, 975, 1236, 1247
AclWI	GGATC	4 cut(s)	456, 800, 815, 828
AcsI	RAATTY	2 cut(s)	342, 521
AcuI	CTGAAG	1 cut(s)	38
AfaI	GTAC	4 cut(s)	431, 546, 685, 1159
AfeI	AGCGCT	1 cut(s)	1257
AfiI	CCNNNNNNNGG	3 cut(s)	57, 235, 832
AgsI	TTSAA	6 cut(s)	314, 506, 636, 754, 856, 1154
AhlI	ACTAGT	1 cut(s)	730
AjnI	CCWGG	2 cut(s)	993, 1170
AjuI	GAANNNNNNNTTGG	2 cut(s)	1031, 1063
AluBI	AGCT	7 cut(s)	25, 73, 155, 620, 664, 737, 911
AluI	AGCT	7 cut(s)	25, 73, 155, 620, 664, 737, 911
Alw26I	GTCTC	2 cut(s)	278, 668
AlwI	GGATC	4 cut(s)	456, 800, 815, 828
AlwNI	CAGNNNCTG	1 cut(s)	716
Ama87I	CYCGRG	1 cut(s)	825
Aor51HI	AGCGCT	1 cut(s)	1257
AoxI	GGCC	1 cut(s)	463
ApoI	RAATTY	2 cut(s)	342, 521
ArsI	GACNNNNNNTTYG	2 cut(s)	114, 146
Asp700I	GAANNNNTTC	1 cut(s)	982
Asp718I	GGTACC	2 cut(s)	544, 1157
AspLEI	GCGC	2 cut(s)	1207, 1258
AspS9I	GGNCC	2 cut(s)	365, 464
AsuHPI	GGTGA	5 cut(s)	16, 553, 904, 1048, 1150
AvaI	CYCGRG	1 cut(s)	825
AvaII	GGWCC	1 cut(s)	365
AxyI	CCTNAGG	1 cut(s)	369
BaeI	ACNNNNGTAYC	2 cut(s)	1149, 1182
BamHI	GGATCC	1 cut(s)	820
BanI	GGYRCC	2 cut(s)	544, 1157
BbsI	GAAGAC	1 cut(s)	513
BccI	CCATC	3 cut(s)	593, 817, 866
BcgI	CGANNNNNNTGC	2 cut(s)	752, 786
BciT130I	CCWGG	2 cut(s)	995, 1172
BcoDI	GTCTC	2 cut(s)	278, 668
BcuI	ACTAGT	1 cut(s)	730
BfaI	CTAG	3 cut(s)	731, 965, 1127
BfoI	RGCGCY	2 cut(s)	1208, 1259
BglII	AGATCT	1 cut(s)	1222
Bme1390I	CCNGG	2 cut(s)	995, 1172
Bme18I	GGWCC	1 cut(s)	365
BmeT110I	CYCGRG	1 cut(s)	825
BmgT120I	GGNCC	2 cut(s)	365, 464
BmiI	GGNNCC	6 cut(s)	160, 496, 546, 609, 822, 1159
BmrFI	CCNGG	2 cut(s)	995, 1172
BmsI	GCATC	5 cut(s)	181, 321, 571, 733, 1222
BpiI	GAAGAC	1 cut(s)	513
BpuEI	CTTGAG	2 cut(s)	260, 1172
Bsa29I	ATCGAT	2 cut(s)	552, 639
BsaI	GGTCTC	1 cut(s)	278
BsaJI	CCNNGG	2 cut(s)	201, 1171
BsaWI	WCCGGW	1 cut(s)	563
Bsc4I	CCNNNNNNNGG	3 cut(s)	57, 235, 832
Bse118I	RCCGGY	1 cut(s)	1135
Bse1I	ACTGG	1 cut(s)	1122
Bse21I	CCTNAGG	1 cut(s)	369
BseBI	CCWGG	2 cut(s)	995, 1172
BseCI	ATCGAT	2 cut(s)	552, 639
BseDI	CCNNGG	2 cut(s)	201, 1171
BseGI	GGATG	2 cut(s)	87, 196
BseLI	CCNNNNNNNGG	3 cut(s)	57, 235, 832
BseMII	CTCAG	2 cut(s)	383, 507
BseNI	ACTGG	1 cut(s)	1122
BsgI	GTGCAG	1 cut(s)	921
Bsh1236I	CGCG	1 cut(s)	804
BshFI	GGCC	1 cut(s)	465
BshNI	GGYRCC	2 cut(s)	544, 1157
BshVI	ATCGAT	2 cut(s)	552, 639
BsiHKCI	CYCGRG	1 cut(s)	825
BsiSI	CCGG	3 cut(s)	51, 564, 1136
BslFI	GGGAC	2 cut(s)	122, 281
BslI	CCNNNNNNNGG	3 cut(s)	57, 235, 832
BsmAI	GTCTC	2 cut(s)	278, 668
BsmFI	GGGAC	2 cut(s)	122, 281
BsnI	GGCC	1 cut(s)	465
Bso31I	GGTCTC	1 cut(s)	278
BsoBI	CYCGRG	1 cut(s)	825
Bsp1407I	TGTACA	1 cut(s)	683
Bsp143I	GATC	6 cut(s)	448, 529, 640, 805, 820, 1222
Bsp68I	TCGCGA	1 cut(s)	804
BspACI	CCGC	6 cut(s)	34, 76, 79, 975, 1236, 1247
BspANI	GGCC	1 cut(s)	465
BspCNI	CTCAG	2 cut(s)	382, 508
BspDI	ATCGAT	2 cut(s)	552, 639
BspFNI	CGCG	1 cut(s)	804
BspLI	GGNNCC	6 cut(s)	160, 496, 546, 609, 822, 1159
BspPI	GGATC	4 cut(s)	456, 800, 815, 828
BspQI	GCTCTTC	1 cut(s)	804
BspT107I	GGYRCC	2 cut(s)	544, 1157
BspTNI	GGTCTC	1 cut(s)	278
BsrBI	CCGCTC	1 cut(s)	36
BsrFI	RCCGGY	1 cut(s)	1135
BsrGI	TGTACA	1 cut(s)	683
BsrI	ACTGG	1 cut(s)	1122
BssAI	RCCGGY	1 cut(s)	1135
BssECI	CCNNGG	2 cut(s)	201, 1171
BssMI	GATC	6 cut(s)	448, 529, 640, 805, 820, 1222
BssT1I	CCWWGG	1 cut(s)	201
Bst2UI	CCWGG	2 cut(s)	995, 1172
Bst4CI	ACNGT	1 cut(s)	683
Bst6I	CTCTTC	2 cut(s)	241, 804
BstAPI	GCANNNNNTGC	1 cut(s)	716
BstAUI	TGTACA	1 cut(s)	683
BstC8I	GCNNGC	2 cut(s)	325, 463
BstDEI	CTNAG	3 cut(s)	369, 516, 868
BstF5I	GGATG	2 cut(s)	87, 196
BstFNI	CGCG	1 cut(s)	804
BstH2I	RGCGCY	2 cut(s)	1208, 1259
BstHHI	GCGC	2 cut(s)	1207, 1258
BstKTI	GATC	6 cut(s)	451, 532, 643, 808, 823, 1225
BstMAI	GTCTC	2 cut(s)	278, 668
BstMBI	GATC	6 cut(s)	448, 529, 640, 805, 820, 1222
BstMWI	GCNNNNNNNGC	7 cut(s)	70, 716, 743, 845, 908, 1244, 1253
BstNI	CCWGG	2 cut(s)	995, 1172
BstSCI	CCNGG	2 cut(s)	993, 1170
BstUI	CGCG	1 cut(s)	804
BstV2I	GAAGAC	1 cut(s)	513
BstX2I	RGATCY	2 cut(s)	820, 1222
BstYI	RGATCY	2 cut(s)	820, 1222
Bsu15I	ATCGAT	2 cut(s)	552, 639
Bsu36I	CCTNAGG	1 cut(s)	369
BsuRI	GGCC	1 cut(s)	465
BsuTUI	ATCGAT	2 cut(s)	552, 639
BtgZI	GCGATG	1 cut(s)	795
BtsCI	GGATG	2 cut(s)	87, 196
BtsI	GCAGTG	1 cut(s)	686
BtsIMutI	CAGTG	2 cut(s)	676, 686
BtuMI	TCGCGA	1 cut(s)	804
Cac8I	GCNNGC	2 cut(s)	325, 463
CaiI	CAGNNNCTG	1 cut(s)	716
CfoI	GCGC	2 cut(s)	1207, 1258
Cfr10I	RCCGGY	1 cut(s)	1135
Cfr13I	GGNCC	2 cut(s)	365, 464
ClaI	ATCGAT	2 cut(s)	552, 639
CsiI	ACCWGGT	1 cut(s)	993
Csp6I	GTAC	4 cut(s)	430, 545, 684, 1158
CspCI	CAANNNNNGTGG	2 cut(s)	1177, 1212
CviAII	CATG	1 cut(s)	889
CviQI	GTAC	4 cut(s)	430, 545, 684, 1158
DdeI	CTNAG	3 cut(s)	369, 516, 868
DpnI	GATC	6 cut(s)	450, 531, 642, 807, 822, 1224
DpnII	GATC	6 cut(s)	448, 529, 640, 805, 820, 1222
DraI	TTTAAA	1 cut(s)	265
Eam1104I	CTCTTC	2 cut(s)	241, 804
EarI	CTCTTC	2 cut(s)	241, 804
EciI	GGCGGA	2 cut(s)	65, 990
Eco130I	CCWWGG	1 cut(s)	201
Eco31I	GGTCTC	1 cut(s)	278
Eco32I	GATATC	2 cut(s)	319, 928
Eco47I	GGWCC	1 cut(s)	365
Eco47III	AGCGCT	1 cut(s)	1257
Eco57I	CTGAAG	1 cut(s)	38
Eco81I	CCTNAGG	1 cut(s)	369
Eco88I	CYCGRG	1 cut(s)	825
EcoO109I	RGGNCCY	1 cut(s)	365
EcoRII	CCWGG	2 cut(s)	993, 1170
EcoRV	GATATC	2 cut(s)	319, 928
EcoT14I	CCWWGG	1 cut(s)	201
EcoT22I	ATGCAT	1 cut(s)	196
ErhI	CCWWGG	1 cut(s)	201
FaeI	CATG	1 cut(s)	892
FaiI	YATR	8 cut(s)	473, 658, 660, 861, 890, 914, 923, 1058
FalI	AAGNNNNNCTT	2 cut(s)	740, 772
FaqI	GGGAC	2 cut(s)	122, 281
FatI	CATG	1 cut(s)	888
FauI	CCCGC	1 cut(s)	41
FokI	GGATG	2 cut(s)	74, 203
FspBI	CTAG	3 cut(s)	731, 965, 1127
GlaI	GCGC	2 cut(s)	1206, 1257
HaeII	RGCGCY	2 cut(s)	1208, 1259
HaeIII	GGCC	1 cut(s)	465
HapII	CCGG	3 cut(s)	51, 564, 1136
HhaI	GCGC	2 cut(s)	1207, 1258
Hin1II	CATG	1 cut(s)	892
Hin6I	GCGC	2 cut(s)	1205, 1256
HinP1I	GCGC	2 cut(s)	1205, 1256
HincII	GTYRAC	1 cut(s)	1168
HindII	GTYRAC	1 cut(s)	1168
HinfI	GANTC	1 cut(s)	1183
HpaII	CCGG	3 cut(s)	51, 564, 1136
HphI	GGTGA	5 cut(s)	16, 553, 904, 1048, 1150
Hpy166II	GTNNAC	3 cut(s)	289, 686, 1168
Hpy188I	TCNGA	4 cut(s)	534, 783, 988, 1027
Hpy188III	TCNNGA	7 cut(s)	139, 188, 298, 446, 803, 965, 1226
Hpy8I	GTNNAC	3 cut(s)	289, 686, 1168
Hpy99I	CGWCG	2 cut(s)	50, 167
HpyAV	CCTTC	2 cut(s)	1027, 1148
HpyCH4III	ACNGT	1 cut(s)	683
HpyCH4V	TGCA	7 cut(s)	194, 485, 587, 710, 888, 902, 1091
HpyF10VI	GCNNNNNNNGC	7 cut(s)	70, 716, 743, 845, 908, 1244, 1253
HpyF3I	CTNAG	3 cut(s)	369, 516, 868
Hsp92II	CATG	1 cut(s)	892
HspAI	GCGC	2 cut(s)	1205, 1256
KpnI	GGTACC	2 cut(s)	548, 1161
Kzo9I	GATC	6 cut(s)	448, 529, 640, 805, 820, 1222
LguI	GCTCTTC	1 cut(s)	804
LmnI	GCTCC	3 cut(s)	78, 613, 617
LweI	GCATC	5 cut(s)	181, 321, 571, 733, 1222
MabI	ACCWGGT	1 cut(s)	993
MaeI	CTAG	3 cut(s)	731, 965, 1127
MaeIII	GTNAC	3 cut(s)	349, 411, 936
MalI	GATC	6 cut(s)	450, 531, 642, 807, 822, 1224
MbiI	CCGCTC	1 cut(s)	36
MboI	GATC	6 cut(s)	448, 529, 640, 805, 820, 1222
MboII	GAAGA	7 cut(s)	258, 326, 518, 590, 661, 791, 1232
MflI	RGATCY	2 cut(s)	820, 1222
MnlI	CCTC	8 cut(s)	40, 92, 242, 378, 811, 823, 997, 1173
Mph1103I	ATGCAT	1 cut(s)	196
MroXI	GAANNNNTTC	1 cut(s)	982
MseI	TTAA	7 cut(s)	222, 264, 540, 933, 1011, 1122, 1251
MspA1I	CMGCKG	3 cut(s)	25, 155, 1238
MspI	CCGG	3 cut(s)	51, 564, 1136
MspR9I	CCNGG	2 cut(s)	995, 1172
MvaI	CCWGG	2 cut(s)	995, 1172
MvnI	CGCG	1 cut(s)	804
MwoI	GCNNNNNNNGC	7 cut(s)	70, 716, 743, 845, 908, 1244, 1253
NdeII	GATC	6 cut(s)	448, 529, 640, 805, 820, 1222
NlaIII	CATG	1 cut(s)	892
NlaIV	GGNNCC	6 cut(s)	160, 496, 546, 609, 822, 1159
NmuCI	GTSAC	1 cut(s)	411
NruI	TCGCGA	1 cut(s)	804
NsiI	ATGCAT	1 cut(s)	196
PciSI	GCTCTTC	1 cut(s)	804
PdmI	GAANNNNTTC	1 cut(s)	982
PfeI	GAWTC	1 cut(s)	1183
PflMI	CCANNNNNTGG	1 cut(s)	235
PpuMI	RGGWCCY	1 cut(s)	365
PsiI	TTATAA	1 cut(s)	473
Psp5II	RGGWCCY	1 cut(s)	365
Psp6I	CCWGG	2 cut(s)	993, 1170
PspGI	CCWGG	2 cut(s)	993, 1170
PspN4I	GGNNCC	6 cut(s)	160, 496, 546, 609, 822, 1159
PspPI	GGNCC	2 cut(s)	365, 464
PspPPI	RGGWCCY	1 cut(s)	365
PstNI	CAGNNNCTG	1 cut(s)	716
PsuI	RGATCY	2 cut(s)	820, 1222
PvuII	CAGCTG	2 cut(s)	25, 155
RruI	TCGCGA	1 cut(s)	804
RsaI	GTAC	4 cut(s)	431, 546, 685, 1159
RsaNI	GTAC	4 cut(s)	430, 545, 684, 1158
SapI	GCTCTTC	1 cut(s)	804
SaqAI	TTAA	7 cut(s)	222, 264, 540, 933, 1011, 1122, 1251
Sau3AI	GATC	6 cut(s)	448, 529, 640, 805, 820, 1222
Sau96I	GGNCC	2 cut(s)	365, 464
ScrFI	CCNGG	2 cut(s)	995, 1172
SexAI	ACCWGGT	1 cut(s)	993
SfaNI	GCATC	5 cut(s)	181, 321, 571, 733, 1222
SinI	GGWCC	1 cut(s)	365
SmlI	CTYRAG	2 cut(s)	275, 1187
SmoI	CTYRAG	2 cut(s)	275, 1187
SpeI	ACTAGT	1 cut(s)	730
SsiI	CCGC	6 cut(s)	34, 76, 79, 975, 1236, 1247
SspMI	CTAG	3 cut(s)	731, 965, 1127
StyD4I	CCNGG	2 cut(s)	993, 1170
StyI	CCWWGG	1 cut(s)	201
TaaI	ACNGT	1 cut(s)	683
TaqI	TCGA	3 cut(s)	165, 552, 639
TatI	WGTACW	2 cut(s)	429, 683
TauI	GCSGC	2 cut(s)	81, 1238
TfiI	GAWTC	1 cut(s)	1183
Tru1I	TTAA	7 cut(s)	222, 264, 540, 933, 1011, 1122, 1251
Tru9I	TTAA	7 cut(s)	222, 264, 540, 933, 1011, 1122, 1251
TscAI	CASTG	2 cut(s)	676, 693
TseFI	GTSAC	1 cut(s)	411
Tsp45I	GTSAC	1 cut(s)	411
TspDTI	ATGAA	3 cut(s)	183, 591, 1088
TspGWI	ACGGA	2 cut(s)	34, 151
TspRI	CASTG	2 cut(s)	676, 693
Van91I	CCANNNNNTGG	1 cut(s)	235
VpaK11BI	GGWCC	1 cut(s)	365
XapI	RAATTY	2 cut(s)	342, 521
XbaI	TCTAGA	1 cut(s)	964
XcmI	CCANNNNNNNNNTGG	1 cut(s)	236
XmnI	GAANNNNTTC	1 cut(s)	982
XspI	CTAG	3 cut(s)	731, 965, 1127
Zsp2I	ATGCAT	1 cut(s)	196

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.