Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr10

Orthogroup ID

OG0010207

Physical Location & Seq

Genomic Coordinates Forward (+)

27309067 .. 27315201

Gene Structure 6135 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD10G1179600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1269 bp

ATGGTGAGAGGATTACTGAAGCAGCTGACCTTCCGCTCTCTCTCCGTCGCCGGAAAATGGCAGCAGCAACAGCTCCGCCGCCTCAACATCCACGAGTATCAGGGGGCTGAATTGATGAGCAAATACGGGGTCAATGTCCCAAAAGGTGTTGCGGCTGGTTCCGTCGATGAAGTCAAAAAGGCAATTCAGGATGTATTCCCCAAGGAAAGTGAGTTGGTGGTTAAAAGCCAAGTCTTGGCTGGTGGAAGAGGCTTGGGAACGTTTAAAAATGGTCTCAAGGGCGGTGTTCACATTGTCCCGACCAACCAAGCTGAAGATATCGCTGGCAAGATGCTTGGGCAAATTCTTGTTACCAAACAAACAGGTCCTCAGGGAAAAATTGTCAGCAAGGTTTATTTGTGCCAAAAGTTGTCACTTGTCAATGAGATGTACTTTTCTATCATTCTGGATCGCAAATCGGCTGGCCCACTTATAATTGCCTGTGCAAAGGGTGGAACCAGCATTGAAGACCTTGCTGAGAAATTCCCTGATCAGATAATTAAGGTACCTATCGATGTTTTCACCGGAATTACAGATGAAGATGCTGCAAAGGTTGTTGATGGTTTGGCTCCAAAAGGGGCTGATAGGAATAGTTCAATCGATCAAGTGAAAAAATTATATAAGCTATTCAGTGAGACTGACTGTACATTGCTGGAAATCAATCCACTTGCAGAGACTGCTGATAACCAGTTAGTAGCTGCTGATGCCAAGTTGAACTTTGACGACAATGCTGCTTATCGTCAGAAAGAGATATTTGCTCTACGTGATCCATCGCAGGAGGATCCCCGAGAGGTGGCTGCTGCCAAAGCAAATTTGAATTATATTGGCTTAGATGGGGAGATTGGTTGCATGGTGAATGGTGCAGGATTAGCTATGGCTACAATGGATATCATTAAATTACATGGCGGAACTCCTGCCAATTTTCTCGATGTAGGCGGAAATGCTTCGGAAAACCAGGTTGTTGAGGCATTTAAGATTTTGACTTCTGATGAGAAGGTGAAGGCGATTTTGGTCAATATCTTTGGTGGAATAATGAAATGTGATGTGATTGCAAGTGGAATTGTCAATGCTGCTAAACAGGTTAAACTAGAAGTGCCAGTGGTTGTTCGTCTTGAAGGTACCAATGTCGACCAAGGAAAGACGATTCTCAAGGAAAGTGGAATGGCACTAATTACAGCCGAAGATTTGGACGATGCTGCTGATAAAGCGGTTAAAGCACTGAAGAAATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000166 GO:0003674 GO:0003824 GO:0004774 GO:0004775 GO:0005488 GO:0005507 GO:0005524 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0006082 GO:0006091 GO:0006099 GO:0006101 GO:0006104 GO:0006105 GO:0006139 GO:0006163 GO:0006637 GO:0006725 GO:0006732 GO:0006753 GO:0006790 GO:0006793 GO:0006796 GO:0006807 GO:0008144 GO:0008150 GO:0008152 GO:0009060 GO:0009117 GO:0009150 GO:0009259 GO:0009987 GO:0010035 GO:0010038 GO:0015980 GO:0016874 GO:0016877 GO:0016878 GO:0016999 GO:0017076 GO:0017144 GO:0019637 GO:0019693 GO:0019752 GO:0030554 GO:0032553 GO:0032555 GO:0032559 GO:0033865 GO:0033875 GO:0034032 GO:0034641 GO:0035383 GO:0035639 GO:0036094 GO:0042221 GO:0043167 GO:0043168 GO:0043169 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0043603 GO:0043648 GO:0044237 GO:0044238 GO:0044281 GO:0044424 GO:0044444 GO:0044464 GO:0045333 GO:0046483 GO:0046686 GO:0046872 GO:0046914 GO:0050896 GO:0051186 GO:0055086 GO:0055114 GO:0071704 GO:0072350 GO:0072521 GO:0097159 GO:0097367 GO:1901135 GO:1901265 GO:1901360 GO:1901363 GO:1901564

KEGG Pathways

Metabolic & Signaling

ko00020 ko00640 ko01100 ko01110 ko01120 ko01130 ko01200 map00020 map00640 map01100 map01110 map01120 map01130 map01200

Pfam Domains

Protein Families

ATP-grasp_2 Ligase_CoA

Protein Analysis

423

Amino Acids

45.19

Weight (kDa)

6.09

Isoelectric Point (pI)

27.69

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
ATP-grasp_2	PF08442	29 - 237	4.6e-60	ATP-grasp domain
Citrate_synth_N	PF24948	30 - 262	5.4e-12	ATP-citrate synthase ATP-grasp domain
Ligase_CoA	PF00549	297 - 417	1.5e-36	CoA-ligase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0010207)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G20420
fragaria_vesca	FvH4_2g20990 FvH4_2g20990 FvH4_2g20990
malus_domestica	MD05G1193100.v1.1 MD10G1179600.v1.1
prunus_persica	Prupe.8G219400_v2.0.a1 Prupe.8G219400_v2.0.a1
pyrus_communis	pycom05g18000 pycom10g15570
rosa_chinensis	RchiOBHm_Chr6g0287371
rosa_laevigata	RLG00000012494
rosa_multiflora	Rmu_sc0007029.1_g000025
rosa_roxburghii	Rroxscaffold_7G00181490
rosa_rugosa	Rorug06G0187700.1
rosa_samantha	Rh6AG298900 Rh6BG304500 Rh6CG305200 Rh6CG313000 Rh6DG296300
rosa_wichuraiana	Rw6G025830

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	473
Acc65I	GGTACC	2 cut(s)	544, 1157
AccB1I	GGYRCC	2 cut(s)	544, 1157
AccB7I	CCANNNNNTGG	1 cut(s)	235
AccBSI	CCGCTC	1 cut(s)	36
AccI	GTMKAC	1 cut(s)	1167
AciI	CCGC	8 cut(s)	34, 76, 79, 152, 282, 945, 975, 1247
AclI	AACGTT	1 cut(s)	260
AclWI	GGATC	4 cut(s)	456, 800, 815, 828
AcsI	RAATTY	3 cut(s)	342, 521, 850
AcuI	CTGAAG	2 cut(s)	38, 333
AfaI	GTAC	4 cut(s)	431, 546, 685, 1159
AfiI	CCNNNNNNNGG	3 cut(s)	57, 235, 832
AgsI	TTSAA	5 cut(s)	506, 636, 754, 856, 1154
AjnI	CCWGG	1 cut(s)	993
AjuI	GAANNNNNNNTTGG	2 cut(s)	1031, 1063
AluBI	AGCT	6 cut(s)	25, 73, 311, 664, 737, 911
AluI	AGCT	6 cut(s)	25, 73, 311, 664, 737, 911
Alw26I	GTCTC	3 cut(s)	278, 668, 707
AlwI	GGATC	4 cut(s)	456, 800, 815, 828
AlwNI	CAGNNNCTG	1 cut(s)	716
Ama87I	CYCGRG	1 cut(s)	825
AoxI	GGCC	1 cut(s)	463
ApoI	RAATTY	3 cut(s)	342, 521, 850
ArsI	GACNNNNNNTTYG	2 cut(s)	114, 146
Asp700I	GAANNNNTTC	1 cut(s)	982
Asp718I	GGTACC	2 cut(s)	544, 1157
AspS9I	GGNCC	2 cut(s)	365, 464
AsuHPI	GGTGA	4 cut(s)	16, 553, 904, 1048
AvaI	CYCGRG	1 cut(s)	825
AvaII	GGWCC	1 cut(s)	365
AxyI	CCTNAGG	1 cut(s)	369
BaeI	ACNNNNGTAYC	2 cut(s)	1149, 1182
BamHI	GGATCC	1 cut(s)	820
BanI	GGYRCC	2 cut(s)	544, 1157
BauI	CACGAG	1 cut(s)	92
BbsI	GAAGAC	1 cut(s)	513
BccI	CCATC	3 cut(s)	593, 817, 866
BcgI	CGANNNNNNTGC	2 cut(s)	752, 786
BciT130I	CCWGG	1 cut(s)	995
BclI	TGATCA	1 cut(s)	529
BcoDI	GTCTC	3 cut(s)	278, 668, 707
BfaI	CTAG	1 cut(s)	1127
Bme1390I	CCNGG	1 cut(s)	995
Bme18I	GGWCC	1 cut(s)	365
BmeT110I	CYCGRG	1 cut(s)	825
BmgT120I	GGNCC	2 cut(s)	365, 464
BmiI	GGNNCC	6 cut(s)	160, 496, 546, 609, 822, 1159
BmrFI	CCNGG	1 cut(s)	995
BmsI	GCATC	4 cut(s)	321, 571, 733, 1222
BpiI	GAAGAC	1 cut(s)	513
BpuEI	CTTGAG	2 cut(s)	260, 1172
Bsa29I	ATCGAT	2 cut(s)	552, 639
BsaAI	YACGTR	1 cut(s)	803
BsaI	GGTCTC	1 cut(s)	278
BsaJI	CCNNGG	2 cut(s)	201, 1171
BsaWI	WCCGGW	1 cut(s)	563
Bsc4I	CCNNNNNNNGG	3 cut(s)	57, 235, 832
Bse1I	ACTGG	2 cut(s)	727, 1136
Bse21I	CCTNAGG	1 cut(s)	369
Bse3DI	GCAATG	1 cut(s)	686
BseBI	CCWGG	1 cut(s)	995
BseCI	ATCGAT	2 cut(s)	552, 639
BseDI	CCNNGG	2 cut(s)	201, 1171
BseGI	GGATG	2 cut(s)	87, 196
BseLI	CCNNNNNNNGG	3 cut(s)	57, 235, 832
BseMI	GCAATG	1 cut(s)	686
BseMII	CTCAG	2 cut(s)	383, 507
BseNI	ACTGG	2 cut(s)	727, 1136
BsgI	GTGCAG	1 cut(s)	921
BshFI	GGCC	1 cut(s)	465
BshNI	GGYRCC	2 cut(s)	544, 1157
BshVI	ATCGAT	2 cut(s)	552, 639
BsiHKCI	CYCGRG	1 cut(s)	825
BsiSI	CCGG	2 cut(s)	51, 564
BslFI	GGGAC	2 cut(s)	122, 281
BslI	CCNNNNNNNGG	3 cut(s)	57, 235, 832
BsmAI	GTCTC	3 cut(s)	278, 668, 707
BsmFI	GGGAC	2 cut(s)	122, 281
BsnI	GGCC	1 cut(s)	465
Bso31I	GGTCTC	1 cut(s)	278
BsoBI	CYCGRG	1 cut(s)	825
Bsp1407I	TGTACA	1 cut(s)	683
Bsp143I	GATC	5 cut(s)	448, 529, 640, 805, 820
BspACI	CCGC	8 cut(s)	34, 76, 79, 152, 282, 945, 975, 1247
BspANI	GGCC	1 cut(s)	465
BspCNI	CTCAG	2 cut(s)	382, 508
BspDI	ATCGAT	2 cut(s)	552, 639
BspLI	GGNNCC	6 cut(s)	160, 496, 546, 609, 822, 1159
BspPI	GGATC	4 cut(s)	456, 800, 815, 828
BspT107I	GGYRCC	2 cut(s)	544, 1157
BspTNI	GGTCTC	1 cut(s)	278
BsrBI	CCGCTC	1 cut(s)	36
BsrDI	GCAATG	1 cut(s)	686
BsrGI	TGTACA	1 cut(s)	683
BsrI	ACTGG	2 cut(s)	727, 1136
BssECI	CCNNGG	2 cut(s)	201, 1171
BssMI	GATC	5 cut(s)	448, 529, 640, 805, 820
BssSI	CACGAG	1 cut(s)	92
BssT1I	CCWWGG	2 cut(s)	201, 1171
Bst2BI	CACGAG	1 cut(s)	92
Bst2UI	CCWGG	1 cut(s)	995
Bst4CI	ACNGT	1 cut(s)	683
Bst6I	CTCTTC	1 cut(s)	241
BstAPI	GCANNNNNTGC	1 cut(s)	716
BstAUI	TGTACA	1 cut(s)	683
BstBAI	YACGTR	1 cut(s)	803
BstC8I	GCNNGC	2 cut(s)	325, 463
BstDEI	CTNAG	3 cut(s)	369, 516, 868
BstF5I	GGATG	2 cut(s)	87, 196
BstKTI	GATC	5 cut(s)	451, 532, 643, 808, 823
BstMAI	GTCTC	3 cut(s)	278, 668, 707
BstMBI	GATC	5 cut(s)	448, 529, 640, 805, 820
BstMWI	GCNNNNNNNGC	7 cut(s)	70, 716, 743, 845, 908, 1244, 1253
BstNI	CCWGG	1 cut(s)	995
BstSCI	CCNGG	1 cut(s)	993
BstV2I	GAAGAC	1 cut(s)	513
BstX2I	RGATCY	1 cut(s)	820
BstYI	RGATCY	1 cut(s)	820
Bsu15I	ATCGAT	2 cut(s)	552, 639
Bsu36I	CCTNAGG	1 cut(s)	369
BsuRI	GGCC	1 cut(s)	465
BsuTUI	ATCGAT	2 cut(s)	552, 639
BtgZI	GCGATG	1 cut(s)	795
BtsCI	GGATG	2 cut(s)	87, 196
BtsIMutI	CAGTG	3 cut(s)	676, 1143, 1256
Cac8I	GCNNGC	2 cut(s)	325, 463
CaiI	CAGNNNCTG	1 cut(s)	716
Cfr13I	GGNCC	2 cut(s)	365, 464
ClaI	ATCGAT	2 cut(s)	552, 639
CsiI	ACCWGGT	1 cut(s)	993
Csp6I	GTAC	4 cut(s)	430, 545, 684, 1158
CspCI	CAANNNNNGTGG	2 cut(s)	1177, 1212
CviAII	CATG	2 cut(s)	889, 941
CviQI	GTAC	4 cut(s)	430, 545, 684, 1158
DdeI	CTNAG	3 cut(s)	369, 516, 868
DpnI	GATC	5 cut(s)	450, 531, 642, 807, 822
DpnII	GATC	5 cut(s)	448, 529, 640, 805, 820
DraI	TTTAAA	1 cut(s)	265
Eam1104I	CTCTTC	1 cut(s)	241
EarI	CTCTTC	1 cut(s)	241
EciI	GGCGGA	3 cut(s)	65, 960, 990
Eco130I	CCWWGG	2 cut(s)	201, 1171
Eco31I	GGTCTC	1 cut(s)	278
Eco32I	GATATC	2 cut(s)	319, 928
Eco47I	GGWCC	1 cut(s)	365
Eco57I	CTGAAG	2 cut(s)	38, 333
Eco81I	CCTNAGG	1 cut(s)	369
Eco88I	CYCGRG	1 cut(s)	825
EcoO109I	RGGNCCY	1 cut(s)	365
EcoRII	CCWGG	1 cut(s)	993
EcoRV	GATATC	2 cut(s)	319, 928
EcoT14I	CCWWGG	2 cut(s)	201, 1171
ErhI	CCWWGG	2 cut(s)	201, 1171
FaeI	CATG	2 cut(s)	892, 944
FaiI	YATR	7 cut(s)	473, 658, 660, 861, 890, 914, 942
FalI	AAGNNNNNCTT	2 cut(s)	740, 772
FaqI	GGGAC	2 cut(s)	122, 281
FatI	CATG	2 cut(s)	888, 940
FbaI	TGATCA	1 cut(s)	529
FblI	GTMKAC	1 cut(s)	1167
FokI	GGATG	2 cut(s)	74, 203
FspBI	CTAG	1 cut(s)	1127
HaeIII	GGCC	1 cut(s)	465
HapII	CCGG	2 cut(s)	51, 564
Hin1II	CATG	2 cut(s)	892, 944
HincII	GTYRAC	1 cut(s)	1168
HindII	GTYRAC	1 cut(s)	1168
HinfI	GANTC	1 cut(s)	1183
HpaII	CCGG	2 cut(s)	51, 564
HphI	GGTGA	4 cut(s)	16, 553, 904, 1048
Hpy166II	GTNNAC	2 cut(s)	289, 1168
Hpy188I	TCNGA	4 cut(s)	534, 783, 988, 1027
Hpy188III	TCNNGA	5 cut(s)	188, 298, 446, 965, 1151
Hpy8I	GTNNAC	2 cut(s)	289, 1168
Hpy99I	CGWCG	2 cut(s)	50, 167
HpyAV	CCTTC	4 cut(s)	40, 1027, 1033, 1148
HpyCH4III	ACNGT	1 cut(s)	683
HpyCH4IV	ACGT	2 cut(s)	260, 802
HpyCH4V	TGCA	6 cut(s)	485, 587, 710, 888, 902, 1091
HpyF10VI	GCNNNNNNNGC	7 cut(s)	70, 716, 743, 845, 908, 1244, 1253
HpyF3I	CTNAG	3 cut(s)	369, 516, 868
HpySE526I	ACGT	2 cut(s)	260, 802
Hsp92II	CATG	2 cut(s)	892, 944
KpnI	GGTACC	2 cut(s)	548, 1161
Ksp22I	TGATCA	1 cut(s)	529
Kzo9I	GATC	5 cut(s)	448, 529, 640, 805, 820
LmnI	GCTCC	2 cut(s)	78, 613
LweI	GCATC	4 cut(s)	321, 571, 733, 1222
MabI	ACCWGGT	1 cut(s)	993
MaeI	CTAG	1 cut(s)	1127
MaeII	ACGT	2 cut(s)	260, 802
MaeIII	GTNAC	2 cut(s)	349, 411
MalI	GATC	5 cut(s)	450, 531, 642, 807, 822
MbiI	CCGCTC	1 cut(s)	36
MboI	GATC	5 cut(s)	448, 529, 640, 805, 820
MboII	GAAGA	5 cut(s)	258, 326, 518, 590, 1232
MflI	RGATCY	1 cut(s)	820
MnlI	CCTC	6 cut(s)	92, 242, 378, 811, 823, 997
MroXI	GAANNNNTTC	1 cut(s)	982
MseI	TTAA	7 cut(s)	222, 264, 540, 933, 1011, 1122, 1251
MspA1I	CMGCKG	1 cut(s)	25
MspI	CCGG	2 cut(s)	51, 564
MspR9I	CCNGG	1 cut(s)	995
MvaI	CCWGG	1 cut(s)	995
MwoI	GCNNNNNNNGC	7 cut(s)	70, 716, 743, 845, 908, 1244, 1253
NdeII	GATC	5 cut(s)	448, 529, 640, 805, 820
NlaIII	CATG	2 cut(s)	892, 944
NlaIV	GGNNCC	6 cut(s)	160, 496, 546, 609, 822, 1159
NmuCI	GTSAC	1 cut(s)	411
PdmI	GAANNNNTTC	1 cut(s)	982
PfeI	GAWTC	1 cut(s)	1183
PflMI	CCANNNNNTGG	1 cut(s)	235
Ppu21I	YACGTR	1 cut(s)	803
PpuMI	RGGWCCY	1 cut(s)	365
PsiI	TTATAA	1 cut(s)	473
Psp1406I	AACGTT	1 cut(s)	260
Psp5II	RGGWCCY	1 cut(s)	365
Psp6I	CCWGG	1 cut(s)	993
PspGI	CCWGG	1 cut(s)	993
PspN4I	GGNNCC	6 cut(s)	160, 496, 546, 609, 822, 1159
PspPI	GGNCC	2 cut(s)	365, 464
PspPPI	RGGWCCY	1 cut(s)	365
PstNI	CAGNNNCTG	1 cut(s)	716
PsuI	RGATCY	1 cut(s)	820
PvuII	CAGCTG	1 cut(s)	25
RsaI	GTAC	4 cut(s)	431, 546, 685, 1159
RsaNI	GTAC	4 cut(s)	430, 545, 684, 1158
SalI	GTCGAC	1 cut(s)	1166
SaqAI	TTAA	7 cut(s)	222, 264, 540, 933, 1011, 1122, 1251
Sau3AI	GATC	5 cut(s)	448, 529, 640, 805, 820
Sau96I	GGNCC	2 cut(s)	365, 464
ScrFI	CCNGG	1 cut(s)	995
SexAI	ACCWGGT	1 cut(s)	993
SfaNI	GCATC	4 cut(s)	321, 571, 733, 1222
SinI	GGWCC	1 cut(s)	365
SmlI	CTYRAG	2 cut(s)	275, 1187
SmoI	CTYRAG	2 cut(s)	275, 1187
SsiI	CCGC	8 cut(s)	34, 76, 79, 152, 282, 945, 975, 1247
SspMI	CTAG	1 cut(s)	1127
StyD4I	CCNGG	1 cut(s)	993
StyI	CCWWGG	2 cut(s)	201, 1171
TaaI	ACNGT	1 cut(s)	683
TaiI	ACGT	2 cut(s)	263, 805
TaqI	TCGA	5 cut(s)	165, 552, 639, 966, 1167
TatI	WGTACW	2 cut(s)	429, 683
TauI	GCSGC	2 cut(s)	81, 155
TfiI	GAWTC	1 cut(s)	1183
Tru1I	TTAA	7 cut(s)	222, 264, 540, 933, 1011, 1122, 1251
Tru9I	TTAA	7 cut(s)	222, 264, 540, 933, 1011, 1122, 1251
TscAI	CASTG	3 cut(s)	676, 1143, 1263
TseFI	GTSAC	1 cut(s)	411
Tsp45I	GTSAC	1 cut(s)	411
TspDTI	ATGAA	3 cut(s)	183, 591, 1088
TspGWI	ACGGA	2 cut(s)	34, 151
TspRI	CASTG	3 cut(s)	676, 1143, 1263
Van91I	CCANNNNNTGG	1 cut(s)	235
VpaK11BI	GGWCC	1 cut(s)	365
XapI	RAATTY	3 cut(s)	342, 521, 850
XcmI	CCANNNNNNNNNTGG	1 cut(s)	236
XmiI	GTMKAC	1 cut(s)	1167
XmnI	GAANNNNTTC	1 cut(s)	982
XspI	CTAG	1 cut(s)	1127

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.