Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr05

Orthogroup ID

OG0004287

Physical Location & Seq

Genomic Coordinates Forward (+)

32176846 .. 32182681

Gene Structure 5836 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD05G1194000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1623 bp

ATGGATATAGACGCAGTAGCAGGGGATGTTGGTTCTTTGGATCCTGAGCTCTTACAGCTGCCGGAATTGTCTCCGTTTGCCCTTAAAACCAGTCCCCAGATTGCTGAGGATTTGTTCGCACAGTGGCTTTCGCTTCCGCAAACCGGCCGTTTGGTGAAGTCTCTAGTCGATGATGCAATAGCAGGAATTCCTATCAATGCCCCTGGGAACTCTTCAAGTGCAAATACTGCCGGGAGCAATGCATTACCTTCCATGTTTCCGGCTGGAAGTACACCTCCACTTTCGCCGAGAAGTTCCTCTGGTTCTCCGCGATTTTCAAAGCTGAAGACTAGTCCTTCTTCTCTTCGCTCTCCGCTAAAATTGGTTAGTGAACCAGTGCGAGAATCTATTCCTCAGTTCTATTTTCAAAACGGTCCACCACCACCAAAGGAACTTAAGGAGCAATGTATTTCTAGAATTGATGACCTCTTCAGTGGTCAGATGGATGGATTGCAACTGCACGAGTTTAAGTTGGTTACAAAGGAGTTATGCAAGCTGCCAACGTTTTTCTCTTCTGCCCTCTTTAGAAAGATTGATACTAGCTGCAGCGGGATAGTGACGAGGGATGCATTCATCAAATATTGGGTCGATGGAAATATGCTGACAATGGATACAACAACTCAAATATTTAAAATTCTAAAGCAATCTGATCGTAATTACCTTACTCAGCTAGACTTCAAACCCATTCTTCTAGAGCTTGTGGCAACCCATCCAGGGTTGGAATTTTTACATGGAACTCCTGAATTTCAAGAAAGATATGCTGAAACTGTAATGTACAGAATATTTTACTACATAAACAGATCAGGAAATGGTCGCCTTACTCTCAGGGAGCTAAAACGAGGAAATTTAATTGCTGCCATGCAACATGCAGATGAGGAAGATGACATTAACAAAGTTCTAAGGTACTTCTCTTATGAACATTTTTATGTCATATATTGCAAATTTTGGGAGCTGGACACAGACCACGATTTCTTGATCGACAAAGAGAATCTTGTTAGATATGGTAATCATGCTCTTACCTACAGGATCGTTGATCGAATATTTTCACAGATTCCGAGAAAGTTTACTAGCAAGGTTGAAGGGAAGATGGGTTATGAAGATTTTGTTTACTTCATGTTGTCAGAGGAGGATAAGTCGTGTGAGCCCAGCCTTGAGTATTGGTTCAGATGTATAGATTTGGATGGAAATGGAGTCCTTACGCCCAATGAGTTGCAGTTCTTTTATGAGGAGCAGCTGCATCGTATGGAATGCATGGCCCAAGAGCCTGTTCTATTCGAGGATATTTTGTGCCAAATAGTTGACATGATTGCACCAGAGAGGGAAGATTATCTCACTGTAGCTGACTTGAAAGGTTGCAAACTTTCAGGGAATGTCTTCAATATCCTTTTCAACCTTAATAAATTCATAGCATTTGAAAGTCGCGATCCTTTTCTTATTCGTCAGGAACGTGAGGATCCAACTTTGACTGAGTGGGACCGCTTTGCACACAGAGAATATATACGGTTGTCAATGGAAGAAGATGTTGAGAATGCCTCTAATGGAAGTGTAGAAGTTTGGGATGAATCACTTGAGGCTCCTTTTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464

KEGG Pathways

Metabolic & Signaling

ko03015 ko04071 ko04151 ko04152 ko04261 ko04728 ko05165 map03015 map04071 map04151 map04152 map04261 map04728 map05165

Pfam Domains

Protein Families

EF-hand_7 EF-hand_8

Protein Analysis

541

Amino Acids

61.95

Weight (kDa)

4.83

Isoelectric Point (pI)

39.94

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
EF-hand_13	PF17958	217 - 306	1.3e-32	EF-hand domain
EF-hand_7	PF13499	322 - 421	6.3e-09	EF-hand domain pair

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0004287)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G03960 AT1G03960 AT1G03960 AT1G03960 AT1G03960 AT1G03960 AT1G03960 AT5G44090 AT5G44090
fragaria_vesca	FvH4_2g21130 FvH4_2g21130 FvH4_2g21130 FvH4_2g21130 FvH4_2g21130
malus_domestica	MD05G1194000.v1.1 MD10G1180700.v1.1
prunus_persica	Prupe.8G220600_v2.0.a1 Prupe.8G220600_v2.0.a1 Prupe.8G220600_v2.0.a1
pyrus_communis	pycom05g18130
rosa_chinensis	RchiOBHm_Chr6g0287621
rosa_laevigata	RLG00000012479
rosa_multiflora	Rmu_sc0004974.1_g000003 Rmu_sc0007029.1_g000005
rosa_roxburghii	Rroxscaffold_7G00181000 Rroxscaffold_7G00181340
rosa_rugosa	Rorug06G0190100 Rorug06G0190200
rosa_samantha	Rh6AG300700 Rh6BG306200 Rh6DG298100
rosa_wichuraiana	Rw6G025940

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccII	CGCG	2 cut(s)	310, 1461
AciI	CCGC	5 cut(s)	137, 308, 353, 588, 1516
AclI	AACGTT	1 cut(s)	542
AclWI	GGATC	6 cut(s)	35, 48, 1073, 1457, 1487, 1500
AcoI	YGGCCR	1 cut(s)	145
AcsI	RAATTY	7 cut(s)	186, 672, 761, 782, 883, 980, 1439
AcuI	CTGAAG	2 cut(s)	344, 454
AfaI	GTAC	3 cut(s)	271, 815, 944
AfiI	CCNNNNNNNGG	2 cut(s)	143, 753
AflII	CTTAAG	1 cut(s)	434
AhlI	ACTAGT	1 cut(s)	329
AjnI	CCWGG	2 cut(s)	202, 751
AjuI	GAANNNNNNNTTGG	2 cut(s)	1290, 1322
Alw21I	GWGCWC	1 cut(s)	51
Alw26I	GTCTC	2 cut(s)	75, 165
AlwI	GGATC	6 cut(s)	35, 48, 1073, 1457, 1487, 1500
AoxI	GGCC	2 cut(s)	145, 1293
ApeKI	GCWGC	7 cut(s)	58, 535, 582, 585, 893, 1270, 1273
ApoI	RAATTY	7 cut(s)	186, 672, 761, 782, 883, 980, 1439
ArsI	GACNNNNNNTTYG	2 cut(s)	609, 641
Asp700I	GAANNNNTTC	3 cut(s)	387, 1081, 1412
AspS9I	GGNCC	3 cut(s)	413, 1294, 1513
AsuC2I	CCSGG	1 cut(s)	232
AsuHPI	GGTGA	1 cut(s)	166
AvaII	GGWCC	2 cut(s)	413, 1513
BamHI	GGATCC	2 cut(s)	40, 1492
BanII	GRGCYC	2 cut(s)	51, 1185
BauI	CACGAG	1 cut(s)	500
BbsI	GAAGAC	2 cut(s)	332, 1405
Bbv12I	GWGCWC	1 cut(s)	51
BbvCI	CCTCAGC	1 cut(s)	105
BbvI	GCAGC	7 cut(s)	45, 522, 569, 597, 880, 1260, 1282
BccI	CCATC	6 cut(s)	475, 479, 623, 756, 1120, 1214
BceAI	ACGGC	1 cut(s)	132
BcgI	CGANNNNNNTGC	4 cut(s)	671, 705, 1259, 1293
BciT130I	CCWGG	2 cut(s)	204, 753
BciVI	GTATCC	1 cut(s)	643
BcnI	CCSGG	1 cut(s)	232
BcoDI	GTCTC	2 cut(s)	75, 165
BcuI	ACTAGT	1 cut(s)	329
BfaI	CTAG	7 cut(s)	164, 330, 453, 579, 710, 731, 1107
BfmI	CTRYAG	3 cut(s)	583, 1060, 1374
BfrI	CTTAAG	1 cut(s)	434
BfuI	GTATCC	1 cut(s)	643
BisI	GCNGC	7 cut(s)	59, 536, 583, 586, 894, 1271, 1274
BlsI	GCNGC	7 cut(s)	60, 537, 584, 587, 895, 1272, 1275
Bme1390I	CCNGG	3 cut(s)	204, 232, 753
Bme18I	GGWCC	2 cut(s)	413, 1513
BmgT120I	GGNCC	3 cut(s)	413, 1294, 1513
BmiI	GGNNCC	4 cut(s)	42, 1494, 1514, 1614
BmrFI	CCNGG	3 cut(s)	204, 232, 753
BmsI	GCATC	3 cut(s)	163, 595, 1285
BpiI	GAAGAC	2 cut(s)	332, 1405
BplI	GAGNNNNNCTC	2 cut(s)	1556, 1588
Bpu10I	CCTNAGC	2 cut(s)	45, 105
BpuEI	CTTGAG	1 cut(s)	1211
BpuMI	CCSGG	1 cut(s)	232
BsaJI	CCNNGG	3 cut(s)	202, 203, 752
BsaXI	ACNNNNNCTCC	2 cut(s)	1157, 1187
Bsc4I	CCNNNNNNNGG	2 cut(s)	143, 753
Bse118I	RCCGGY	1 cut(s)	143
Bse1I	ACTGG	2 cut(s)	90, 374
Bse3DI	GCAATG	2 cut(s)	244, 449
BseBI	CCWGG	2 cut(s)	204, 753
BseDI	CCNNGG	3 cut(s)	202, 203, 752
BseGI	GGATG	6 cut(s)	31, 490, 610, 748, 1225, 1603
BseLI	CCNNNNNNNGG	2 cut(s)	143, 753
BseMI	GCAATG	2 cut(s)	244, 449
BseMII	CTCAG	6 cut(s)	36, 96, 407, 719, 877, 1497
BseNI	ACTGG	2 cut(s)	90, 374
BseRI	GAGGAG	2 cut(s)	1178, 1280
BseX3I	CGGCCG	1 cut(s)	145
BseXI	GCAGC	7 cut(s)	45, 522, 569, 597, 880, 1260, 1282
BseYI	CCCAGC	1 cut(s)	1184
BsgI	GTGCAG	1 cut(s)	482
Bsh1236I	CGCG	2 cut(s)	310, 1461
Bsh1285I	CGRYCG	1 cut(s)	148
BshFI	GGCC	2 cut(s)	147, 1295
BsiEI	CGRYCG	1 cut(s)	148
BsiHKAI	GWGCWC	1 cut(s)	51
BsiSI	CCGG	4 cut(s)	62, 144, 231, 260
BslFI	GGGAC	2 cut(s)	78, 1526
BslI	CCNNNNNNNGG	2 cut(s)	143, 753
BsmAI	GTCTC	2 cut(s)	75, 165
BsmFI	GGGAC	2 cut(s)	78, 1526
BsmI	GAATGC	3 cut(s)	608, 1292, 1573
BsnI	GGCC	2 cut(s)	147, 1295
Bsp1286I	GDGCHC	2 cut(s)	51, 1185
Bsp1407I	TGTACA	1 cut(s)	813
Bsp143I	GATC	8 cut(s)	40, 688, 839, 1014, 1065, 1072, 1462, 1492
Bsp68I	TCGCGA	1 cut(s)	1461
BspACI	CCGC	5 cut(s)	137, 308, 353, 588, 1516
BspANI	GGCC	2 cut(s)	147, 1295
BspCNI	CTCAG	6 cut(s)	37, 97, 406, 718, 876, 1498
BspFNI	CGCG	2 cut(s)	310, 1461
BspLI	GGNNCC	4 cut(s)	42, 1494, 1514, 1614
BspMAI	CTGCAG	1 cut(s)	587
BspPI	GGATC	6 cut(s)	35, 48, 1073, 1457, 1487, 1500
BspTI	CTTAAG	1 cut(s)	434
BsrDI	GCAATG	2 cut(s)	244, 449
BsrFI	RCCGGY	1 cut(s)	143
BsrGI	TGTACA	1 cut(s)	813
BsrI	ACTGG	2 cut(s)	90, 374
BssAI	RCCGGY	1 cut(s)	143
BssECI	CCNNGG	3 cut(s)	202, 203, 752
BssMI	GATC	8 cut(s)	40, 688, 839, 1014, 1065, 1072, 1462, 1492
BssSI	CACGAG	1 cut(s)	500
Bst2BI	CACGAG	1 cut(s)	500
Bst2UI	CCWGG	2 cut(s)	204, 753
Bst4CI	ACNGT	5 cut(s)	123, 413, 808, 1375, 1542
Bst6I	CTCTTC	4 cut(s)	217, 348, 473, 556
BstAFI	CTTAAG	1 cut(s)	434
BstAPI	GCANNNNNTGC	1 cut(s)	227
BstAUI	TGTACA	1 cut(s)	813
BstC8I	GCNNGC	1 cut(s)	533
BstDEI	CTNAG	7 cut(s)	45, 105, 393, 705, 863, 938, 1506
BstF5I	GGATG	6 cut(s)	31, 490, 610, 748, 1225, 1603
BstFNI	CGCG	2 cut(s)	310, 1461
BstKTI	GATC	8 cut(s)	43, 691, 842, 1017, 1068, 1075, 1465, 1495
BstMAI	GTCTC	2 cut(s)	75, 165
BstMBI	GATC	8 cut(s)	40, 688, 839, 1014, 1065, 1072, 1462, 1492
BstMCI	CGRYCG	1 cut(s)	148
BstMWI	GCNNNNNNNGC	2 cut(s)	55, 227
BstNI	CCWGG	2 cut(s)	204, 753
BstNSI	RCATGY	1 cut(s)	908
BstSCI	CCNGG	3 cut(s)	202, 230, 751
BstSFI	CTRYAG	3 cut(s)	583, 1060, 1374
BstUI	CGCG	2 cut(s)	310, 1461
BstV1I	GCAGC	7 cut(s)	45, 522, 569, 597, 880, 1260, 1282
BstV2I	GAAGAC	2 cut(s)	332, 1405
BstX2I	RGATCY	2 cut(s)	40, 1492
BstYI	RGATCY	2 cut(s)	40, 1492
BstZI	CGGCCG	1 cut(s)	145
BsuI	GTATCC	1 cut(s)	643
BsuRI	GGCC	2 cut(s)	147, 1295
BtsCI	GGATG	6 cut(s)	31, 490, 610, 748, 1225, 1603
BtsIMutI	CAGTG	4 cut(s)	128, 381, 478, 1371
BtuMI	TCGCGA	1 cut(s)	1461
Cac8I	GCNNGC	1 cut(s)	533
Cfr10I	RCCGGY	1 cut(s)	143
Cfr13I	GGNCC	3 cut(s)	413, 1294, 1513
CseI	GACGC	1 cut(s)	20
Csp6I	GTAC	3 cut(s)	270, 814, 943
CviAII	CATG	8 cut(s)	253, 770, 898, 905, 1049, 1153, 1291, 1342
CviQI	GTAC	3 cut(s)	270, 814, 943
DdeI	CTNAG	7 cut(s)	45, 105, 393, 705, 863, 938, 1506
DpnI	GATC	8 cut(s)	42, 690, 841, 1016, 1067, 1074, 1464, 1494
DpnII	GATC	8 cut(s)	40, 688, 839, 1014, 1065, 1072, 1462, 1492
DraI	TTTAAA	1 cut(s)	670
EaeI	YGGCCR	1 cut(s)	145
EagI	CGGCCG	1 cut(s)	145
Eam1104I	CTCTTC	4 cut(s)	217, 348, 473, 556
EarI	CTCTTC	4 cut(s)	217, 348, 473, 556
Ecl136II	GAGCTC	1 cut(s)	49
EclXI	CGGCCG	1 cut(s)	145
Eco24I	GRGCYC	2 cut(s)	51, 1185
Eco47I	GGWCC	2 cut(s)	413, 1513
Eco52I	CGGCCG	1 cut(s)	145
Eco53kI	GAGCTC	1 cut(s)	49
Eco57I	CTGAAG	2 cut(s)	344, 454
EcoICRI	GAGCTC	1 cut(s)	49
EcoRI	GAATTC	1 cut(s)	186
EcoRII	CCWGG	2 cut(s)	202, 751
EcoT22I	ATGCAT	3 cut(s)	244, 610, 1292
EcoT38I	GRGCYC	2 cut(s)	51, 1185
FaeI	CATG	8 cut(s)	256, 773, 901, 908, 1052, 1156, 1294, 1345
FalI	AAGNNNNNCTT	2 cut(s)	1014, 1046
FaqI	GGGAC	2 cut(s)	78, 1526
FatI	CATG	8 cut(s)	252, 769, 897, 904, 1048, 1152, 1290, 1341
FauI	CCCGC	1 cut(s)	581
Fnu4HI	GCNGC	7 cut(s)	59, 536, 583, 586, 894, 1271, 1274
FokI	GGATG	6 cut(s)	38, 497, 617, 735, 1232, 1610
FriOI	GRGCYC	2 cut(s)	51, 1185
Fsp4HI	GCNGC	7 cut(s)	59, 536, 583, 586, 894, 1271, 1274
FspBI	CTAG	7 cut(s)	164, 330, 453, 579, 710, 731, 1107
GluI	GCNGC	7 cut(s)	59, 536, 583, 586, 894, 1271, 1274
GsaI	CCCAGC	1 cut(s)	1188
HaeIII	GGCC	2 cut(s)	147, 1295
HapII	CCGG	4 cut(s)	62, 144, 231, 260
HgaI	GACGC	1 cut(s)	20
Hin1II	CATG	8 cut(s)	256, 773, 901, 908, 1052, 1156, 1294, 1345
HincII	GTYRAC	1 cut(s)	1339
HindII	GTYRAC	1 cut(s)	1339
HinfI	GANTC	5 cut(s)	383, 1027, 1090, 1230, 1601
HpaII	CCGG	4 cut(s)	62, 144, 231, 260
HphI	GGTGA	1 cut(s)	166
Hpy166II	GTNNAC	6 cut(s)	272, 371, 416, 1104, 1147, 1339
Hpy188I	TCNGA	5 cut(s)	480, 688, 1095, 1162, 1205
Hpy188III	TCNNGA	9 cut(s)	44, 453, 731, 779, 788, 843, 1012, 1460, 1481
Hpy8I	GTNNAC	6 cut(s)	272, 371, 416, 1104, 1147, 1339
HpyAV	CCTTC	3 cut(s)	258, 345, 1112
HpyCH4III	ACNGT	5 cut(s)	123, 413, 808, 1375, 1542
HpyCH4IV	ACGT	2 cut(s)	542, 1486
HpyF10VI	GCNNNNNNNGC	2 cut(s)	55, 227
HpyF3I	CTNAG	7 cut(s)	45, 105, 393, 705, 863, 938, 1506
HpySE526I	ACGT	2 cut(s)	542, 1486
Hsp92II	CATG	8 cut(s)	256, 773, 901, 908, 1052, 1156, 1294, 1345
Kzo9I	GATC	8 cut(s)	40, 688, 839, 1014, 1065, 1072, 1462, 1492
LmnI	GCTCC	6 cut(s)	234, 439, 868, 988, 1267, 1618
Lsp1109I	GCAGC	7 cut(s)	45, 522, 569, 597, 880, 1260, 1282
LweI	GCATC	3 cut(s)	163, 595, 1285
MaeI	CTAG	7 cut(s)	164, 330, 453, 579, 710, 731, 1107
MaeII	ACGT	2 cut(s)	542, 1486
MaeIII	GTNAC	2 cut(s)	514, 595
MalI	GATC	8 cut(s)	42, 690, 841, 1016, 1067, 1074, 1464, 1494
MboI	GATC	8 cut(s)	40, 688, 839, 1014, 1065, 1072, 1462, 1492
MflI	RGATCY	2 cut(s)	40, 1492
MhlI	GDGCHC	2 cut(s)	51, 1185
MlyI	GAGTC	1 cut(s)	1239
MmeI	TCCRAC	2 cut(s)	738, 1520
Mph1103I	ATGCAT	3 cut(s)	244, 610, 1292
MroXI	GAANNNNTTC	3 cut(s)	387, 1081, 1412
MseI	TTAA	8 cut(s)	84, 435, 507, 669, 887, 927, 1434, 1621
MslI	CAYNNNNRTG	2 cut(s)	909, 963
MspA1I	CMGCKG	3 cut(s)	58, 588, 1273
MspCI	CTTAAG	1 cut(s)	434
MspI	CCGG	4 cut(s)	62, 144, 231, 260
MspR9I	CCNGG	3 cut(s)	204, 232, 753
Mva1269I	GAATGC	3 cut(s)	608, 1292, 1573
MvaI	CCWGG	2 cut(s)	204, 753
MvnI	CGCG	2 cut(s)	310, 1461
MwoI	GCNNNNNNNGC	2 cut(s)	55, 227
NciI	CCSGG	1 cut(s)	232
NdeII	GATC	8 cut(s)	40, 688, 839, 1014, 1065, 1072, 1462, 1492
NlaIII	CATG	8 cut(s)	256, 773, 901, 908, 1052, 1156, 1294, 1345
NlaIV	GGNNCC	4 cut(s)	42, 1494, 1514, 1614
NmeAIII	GCCGAG	1 cut(s)	312
NmuCI	GTSAC	1 cut(s)	595
NruI	TCGCGA	1 cut(s)	1461
NsiI	ATGCAT	3 cut(s)	244, 610, 1292
NspI	RCATGY	1 cut(s)	908
PasI	CCCWGGG	1 cut(s)	203
PcsI	WCGNNNNNNNCGW	1 cut(s)	1483
PctI	GAATGC	3 cut(s)	608, 1292, 1573
PdmI	GAANNNNTTC	3 cut(s)	387, 1081, 1412
PfeI	GAWTC	4 cut(s)	383, 1027, 1090, 1601
PkrI	GCNGC	7 cut(s)	60, 537, 584, 587, 895, 1272, 1275
PleI	GAGTC	1 cut(s)	1238
PpsI	GAGTC	1 cut(s)	1238
Psp124BI	GAGCTC	1 cut(s)	51
Psp1406I	AACGTT	1 cut(s)	542
Psp6I	CCWGG	2 cut(s)	202, 751
PspFI	CCCAGC	1 cut(s)	1184
PspGI	CCWGG	2 cut(s)	202, 751
PspN4I	GGNNCC	4 cut(s)	42, 1494, 1514, 1614
PspPI	GGNCC	3 cut(s)	413, 1294, 1513
PstI	CTGCAG	1 cut(s)	587
PsuI	RGATCY	2 cut(s)	40, 1492
PvuII	CAGCTG	2 cut(s)	58, 1273
RruI	TCGCGA	1 cut(s)	1461
RsaI	GTAC	3 cut(s)	271, 815, 944
RsaNI	GTAC	3 cut(s)	270, 814, 943
RseI	CAYNNNNRTG	2 cut(s)	909, 963
SacI	GAGCTC	1 cut(s)	51
SaqAI	TTAA	8 cut(s)	84, 435, 507, 669, 887, 927, 1434, 1621
SatI	GCNGC	7 cut(s)	59, 536, 583, 586, 894, 1271, 1274
Sau3AI	GATC	8 cut(s)	40, 688, 839, 1014, 1065, 1072, 1462, 1492
Sau96I	GGNCC	3 cut(s)	413, 1294, 1513
SchI	GAGTC	1 cut(s)	1239
ScrFI	CCNGG	3 cut(s)	204, 232, 753
SduI	GDGCHC	2 cut(s)	51, 1185
SfaNI	GCATC	3 cut(s)	163, 595, 1285
SfcI	CTRYAG	3 cut(s)	583, 1060, 1374
SinI	GGWCC	2 cut(s)	413, 1513
SmiMI	CAYNNNNRTG	2 cut(s)	909, 963
SmlI	CTYRAG	3 cut(s)	434, 1190, 1607
SmoI	CTYRAG	3 cut(s)	434, 1190, 1607
SpeI	ACTAGT	1 cut(s)	329
SsiI	CCGC	5 cut(s)	137, 308, 353, 588, 1516
SspI	AATATT	4 cut(s)	620, 666, 822, 1080
SspMI	CTAG	7 cut(s)	164, 330, 453, 579, 710, 731, 1107
SstI	GAGCTC	1 cut(s)	51
StyD4I	CCNGG	3 cut(s)	202, 230, 751
TaaI	ACNGT	5 cut(s)	123, 413, 808, 1375, 1542
TaiI	ACGT	2 cut(s)	545, 1489
TaqI	TCGA	5 cut(s)	168, 627, 1017, 1075, 1314
TatI	WGTACW	2 cut(s)	269, 813
TfiI	GAWTC	4 cut(s)	383, 1027, 1090, 1601
Tru1I	TTAA	8 cut(s)	84, 435, 507, 669, 887, 927, 1434, 1621
Tru9I	TTAA	8 cut(s)	84, 435, 507, 669, 887, 927, 1434, 1621
TscAI	CASTG	4 cut(s)	128, 381, 478, 1378
TseFI	GTSAC	1 cut(s)	595
TseI	GCWGC	7 cut(s)	58, 535, 582, 585, 893, 1270, 1273
Tsp45I	GTSAC	1 cut(s)	595
TspDTI	ATGAA	6 cut(s)	601, 969, 1141, 1149, 1432, 1614
TspGWI	ACGGA	1 cut(s)	63
TspRI	CASTG	4 cut(s)	128, 381, 478, 1378
Vha464I	CTTAAG	1 cut(s)	434
VpaK11BI	GGWCC	2 cut(s)	413, 1513
XapI	RAATTY	7 cut(s)	186, 672, 761, 782, 883, 980, 1439
XbaI	TCTAGA	2 cut(s)	452, 730
XceI	RCATGY	1 cut(s)	908
XmnI	GAANNNNTTC	3 cut(s)	387, 1081, 1412
XspI	CTAG	7 cut(s)	164, 330, 453, 579, 710, 731, 1107
Zsp2I	ATGCAT	3 cut(s)	244, 610, 1292

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.