MD05G1209900.v1.1

JmjC domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0009293
Physical Location & Seq
Forward (+)
34133165 .. 34137367
4203 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1209900.v1.1.491

Sequence Viewer

Length: 1041 bp
ATGGAGGAAATAGAGGCATTGTGGAAAGAAGTAAGGGAGCTAAGTCTAGGCGAGTCGGGAAGAGTGGAGCGGTTGGAGTTCCCACCCACCCCTCTCCAATTCCTTAGAGATTATGTTAACCATAACAAGCCCTGCATTATATCCAATGCCACCCTCCACTGGCCCGCCCTCTCCTCCTGGACCGACGATTCTTATCTCACTGAAGCCCTCTCCTCTGCTGACATTTCCCTTCACCTTACTCCGCACGGCCAGGCTGATGCCCTCGTCCCCTTGGATGGTTCTCTTTGCTTCGCGTCTGCTCACGTGCAGCGCATGCCCTTCCCGAAAGCTCTCAGCCTCATCACCAACAATGAGTCCCAATCGAAACTGGTGGCCTATGCGCAGCAGCAGAACAACTGCTTTCCATCAGAGTACTCGGCTTTAGCTGGGGATTGTGACCCCCATATCCCATGGGCCAGTGAGGCCCTTGGCTGCCTCCCTGATGCAGTCAACATGTGGATTGGCAACCATCTGTCTACAACATCGTTTCATAAGGACCACTATGAGAATCTCTATGCTGTCGTGACTGGGCAGAAGCATTTCCTGCTCCTTCCTCCTACTGATGTGCATCGAATGTACATCCGTGACTACCCATCTGCCCAATACTCATATTCCCATGACACTGGAGAGTTCACGTTGGAACTGGAGAAGCCAGACAGATATGTGCCTTGGTGTAGTGTCGATCCTTACCCTTCTCCCGAGGACAGAGATAAACAGCTCTCTAGCTTTCCTTTGTATTTTGACGGTCCTAAGCCCTTTCACTGTACTGTCAACCCTGGAGAGATTCTATACTTGCCAAGCATGTGGTTTCATCACGTTAGACAAACTCCAGATAGCAGAGGACGCACTATTGCTGTGAACTACTGGTATGATATGCAGTATGACATCAAGTATGCTTACTTCAACTTTTTGCAATCAATTCATTGCCTATCTGTTAAAACTCCAACACTGGTTGAGACAGTGCATGAGGATTCAGGTTCTGATGCATGGGCGTGTATATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cupin_8

Protein Analysis

347

Amino Acids

39.48

Weight (kDa)

5.14

Isoelectric Point (pI)

46.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cupin_8 PF13621 31 - 308 6.4e-74 Cupin-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 381
AccBSI CCGCTC 1 cut(s) 70
AccI GTMKAC 1 cut(s) 515
AccII CGCG 1 cut(s) 293
AciI CCGC 3 cut(s) 70, 165, 242
AclWI GGATC 1 cut(s) 716
AcoI YGGCCR 1 cut(s) 247
AcuI CTGAAG 1 cut(s) 222
AcvI CACGTG 1 cut(s) 304
AfaI GTAC 3 cut(s) 413, 617, 805
AfiI CCNNNNNNNGG 2 cut(s) 159, 275
AflIII ACRYGT 1 cut(s) 492
AgsI TTSAA 1 cut(s) 943
AjnI CCWGG 3 cut(s) 176, 249, 814
AluBI AGCT 5 cut(s) 40, 329, 425, 757, 765
AluI AGCT 5 cut(s) 40, 329, 425, 757, 765
Alw26I GTCTC 1 cut(s) 989
AlwI GGATC 1 cut(s) 716
AlwNI CAGNNNCTG 1 cut(s) 1019
Ama87I CYCGRG 1 cut(s) 737
AoxI GGCC 5 cut(s) 161, 247, 372, 453, 462
ApeKI GCWGC 4 cut(s) 307, 382, 385, 471
Asp700I GAANNNNTTC 1 cut(s) 578
AspLEI GCGC 2 cut(s) 312, 382
AspS9I GGNCC 6 cut(s) 162, 180, 453, 463, 535, 785
AsuHPI GGTGA 2 cut(s) 224, 334
AvaI CYCGRG 1 cut(s) 737
AvaII GGWCC 3 cut(s) 180, 535, 785
BarI GAAGNNNNNNTAC 2 cut(s) 923, 955
BbrPI CACGTG 1 cut(s) 304
BbvI GCAGC 4 cut(s) 319, 394, 397, 458
BccI CCATC 4 cut(s) 269, 412, 516, 640
BceAI ACGGC 1 cut(s) 262
BciT130I CCWGG 3 cut(s) 178, 251, 816
BcoDI GTCTC 1 cut(s) 989
BfaI CTAG 2 cut(s) 47, 762
BglI GCCNNNNNGGC 1 cut(s) 461
BisI GCNGC 4 cut(s) 308, 383, 386, 472
BlsI GCNGC 4 cut(s) 309, 384, 387, 473
BmcAI AGTACT 1 cut(s) 413
Bme1390I CCNGG 3 cut(s) 178, 251, 816
Bme18I GGWCC 3 cut(s) 180, 535, 785
BmeT110I CYCGRG 1 cut(s) 737
BmgT120I GGNCC 6 cut(s) 162, 180, 453, 463, 535, 785
BmrFI CCNGG 3 cut(s) 178, 251, 816
BmrI ACTGGG 1 cut(s) 576
BmsI GCATC 4 cut(s) 247, 472, 616, 1012
BmuI ACTGGG 1 cut(s) 576
BpmI CTGGAG 4 cut(s) 684, 704, 837, 852
Bpu10I CCTNAGC 1 cut(s) 789
BsaAI YACGTR 1 cut(s) 304
BsaBI GATNNNNATC 2 cut(s) 192, 606
BsaJI CCNNGG 6 cut(s) 270, 449, 466, 707, 738, 814
Bsc4I CCNNNNNNNGG 2 cut(s) 159, 275
Bse1I ACTGG 8 cut(s) 164, 372, 456, 571, 667, 687, 908, 993
Bse3DI GCAATG 1 cut(s) 961
Bse8I GATNNNNATC 2 cut(s) 192, 606
BseBI CCWGG 3 cut(s) 178, 251, 816
BseDI CCNNGG 6 cut(s) 270, 449, 466, 707, 738, 814
BseGI GGATG 2 cut(s) 280, 618
BseJI GATNNNNATC 2 cut(s) 192, 606
BseLI CCNNNNNNNGG 2 cut(s) 159, 275
BseMI GCAATG 1 cut(s) 961
BseMII CTCAG 1 cut(s) 346
BseNI ACTGG 8 cut(s) 164, 372, 456, 571, 667, 687, 908, 993
BseRI GAGGAG 2 cut(s) 163, 202
BseXI GCAGC 4 cut(s) 319, 394, 397, 458
BseYI CCCAGC 1 cut(s) 425
BsgI GTGCAG 1 cut(s) 326
Bsh1236I CGCG 1 cut(s) 293
BshFI GGCC 5 cut(s) 163, 249, 374, 455, 464
BsiHKCI CYCGRG 1 cut(s) 737
BslFI GGGAC 2 cut(s) 251, 340
BslI CCNNNNNNNGG 2 cut(s) 159, 275
BsmAI GTCTC 1 cut(s) 989
BsmFI GGGAC 2 cut(s) 251, 340
BsnI GGCC 5 cut(s) 163, 249, 374, 455, 464
BsoBI CYCGRG 1 cut(s) 737
Bsp1407I TGTACA 1 cut(s) 615
Bsp143I GATC 1 cut(s) 721
Bsp19I CCATGG 1 cut(s) 449
BspACI CCGC 3 cut(s) 70, 165, 242
BspANI GGCC 5 cut(s) 163, 249, 374, 455, 464
BspCNI CTCAG 1 cut(s) 345
BspFNI CGCG 1 cut(s) 293
BspPI GGATC 1 cut(s) 716
BsrBI CCGCTC 1 cut(s) 70
BsrDI GCAATG 1 cut(s) 961
BsrGI TGTACA 1 cut(s) 615
BsrI ACTGG 8 cut(s) 164, 372, 456, 571, 667, 687, 908, 993
BssECI CCNNGG 6 cut(s) 270, 449, 466, 707, 738, 814
BssMI GATC 1 cut(s) 721
BssT1I CCWWGG 4 cut(s) 270, 449, 466, 707
Bst2UI CCWGG 3 cut(s) 178, 251, 816
Bst4CI ACNGT 4 cut(s) 785, 803, 808, 1000
Bst6I CTCTTC 1 cut(s) 55
BstAPI GCANNNNNTGC 2 cut(s) 313, 583
BstAUI TGTACA 1 cut(s) 615
BstBAI YACGTR 1 cut(s) 304
BstC8I GCNNGC 2 cut(s) 165, 314
BstDEI CTNAG 4 cut(s) 41, 104, 332, 789
BstDSI CCRYGG 1 cut(s) 449
BstF5I GGATG 2 cut(s) 280, 618
BstFNI CGCG 1 cut(s) 293
BstHHI GCGC 2 cut(s) 312, 382
BstKTI GATC 1 cut(s) 724
BstMAI GTCTC 1 cut(s) 989
BstMBI GATC 1 cut(s) 721
BstMWI GCNNNNNNNGC 4 cut(s) 313, 461, 583, 882
BstNI CCWGG 3 cut(s) 178, 251, 816
BstNSI RCATGY 3 cut(s) 316, 496, 844
BstSCI CCNGG 3 cut(s) 176, 249, 814
BstUI CGCG 1 cut(s) 293
BstV1I GCAGC 4 cut(s) 319, 394, 397, 458
BstXI CCANNNNNNTGG 2 cut(s) 662, 843
BsuRI GGCC 5 cut(s) 163, 249, 374, 455, 464
BtgI CCRYGG 1 cut(s) 449
BtsCI GGATG 2 cut(s) 280, 618
BtsIMutI CAGTG 7 cut(s) 157, 198, 463, 660, 799, 986, 1005
Cac8I GCNNGC 2 cut(s) 165, 314
CaiI CAGNNNCTG 1 cut(s) 1019
CfoI GCGC 2 cut(s) 312, 382
Cfr13I GGNCC 6 cut(s) 162, 180, 453, 463, 535, 785
CseI GACGC 2 cut(s) 282, 891
Csp6I GTAC 3 cut(s) 412, 616, 804
CviAII CATG 7 cut(s) 313, 450, 493, 656, 841, 1004, 1026
CviQI GTAC 3 cut(s) 412, 616, 804
DdeI CTNAG 4 cut(s) 41, 104, 332, 789
DpnI GATC 1 cut(s) 723
DpnII GATC 1 cut(s) 721
EaeI YGGCCR 1 cut(s) 247
Eam1104I CTCTTC 1 cut(s) 55
EarI CTCTTC 1 cut(s) 55
Eco130I CCWWGG 4 cut(s) 270, 449, 466, 707
Eco47I GGWCC 3 cut(s) 180, 535, 785
Eco57I CTGAAG 1 cut(s) 222
Eco72I CACGTG 1 cut(s) 304
Eco88I CYCGRG 1 cut(s) 737
EcoO109I RGGNCCY 1 cut(s) 463
EcoRII CCWGG 3 cut(s) 176, 249, 814
EcoT14I CCWWGG 4 cut(s) 270, 449, 466, 707
EcoT22I ATGCAT 1 cut(s) 1027
ErhI CCWWGG 4 cut(s) 270, 449, 466, 707
FaeI CATG 7 cut(s) 316, 453, 496, 659, 844, 1007, 1029
FaqI GGGAC 2 cut(s) 251, 340
FatI CATG 7 cut(s) 312, 449, 492, 655, 840, 1003, 1025
FauI CCCGC 1 cut(s) 172
FblI GTMKAC 1 cut(s) 515
Fnu4HI GCNGC 4 cut(s) 308, 383, 386, 472
FokI GGATG 2 cut(s) 287, 605
Fsp4HI GCNGC 4 cut(s) 308, 383, 386, 472
FspBI CTAG 2 cut(s) 47, 762
FspI TGCGCA 1 cut(s) 381
GlaI GCGC 2 cut(s) 311, 381
GluI GCNGC 4 cut(s) 308, 383, 386, 472
GsaI CCCAGC 1 cut(s) 429
GsuI CTGGAG 4 cut(s) 684, 704, 837, 852
HaeIII GGCC 5 cut(s) 163, 249, 374, 455, 464
HgaI GACGC 2 cut(s) 282, 891
HhaI GCGC 2 cut(s) 312, 382
Hin1II CATG 7 cut(s) 316, 453, 496, 659, 844, 1007, 1029
Hin6I GCGC 2 cut(s) 310, 380
HinP1I GCGC 2 cut(s) 310, 380
HincII GTYRAC 3 cut(s) 118, 490, 811
HindII GTYRAC 3 cut(s) 118, 490, 811
HinfI GANTC 6 cut(s) 53, 188, 353, 547, 823, 1010
HpaI GTTAAC 1 cut(s) 118
HphI GGTGA 2 cut(s) 224, 334
Hpy166II GTNNAC 6 cut(s) 118, 490, 516, 672, 811, 898
Hpy188I TCNGA 2 cut(s) 409, 1021
Hpy188III TCNNGA 5 cut(s) 57, 322, 562, 737, 869
Hpy8I GTNNAC 6 cut(s) 118, 490, 516, 672, 811, 898
Hpy99I CGWCG 1 cut(s) 188
HpyAV CCTTC 4 cut(s) 239, 328, 599, 741
HpyCH4III ACNGT 4 cut(s) 785, 803, 808, 1000
HpyCH4IV ACGT 3 cut(s) 303, 674, 855
HpyCH4V TGCA 8 cut(s) 135, 307, 485, 607, 916, 952, 1003, 1025
HpyF10VI GCNNNNNNNGC 4 cut(s) 313, 461, 583, 882
HpyF3I CTNAG 4 cut(s) 41, 104, 332, 789
HpySE526I ACGT 3 cut(s) 303, 674, 855
Hsp92II CATG 7 cut(s) 316, 453, 496, 659, 844, 1007, 1029
HspAI GCGC 2 cut(s) 310, 380
KspAI GTTAAC 1 cut(s) 118
Kzo9I GATC 1 cut(s) 721
LmnI GCTCC 3 cut(s) 37, 67, 591
Lsp1109I GCAGC 4 cut(s) 319, 394, 397, 458
LweI GCATC 4 cut(s) 247, 472, 616, 1012
MaeI CTAG 2 cut(s) 47, 762
MaeII ACGT 3 cut(s) 303, 674, 855
MaeIII GTNAC 3 cut(s) 434, 562, 623
MalI GATC 1 cut(s) 723
MbiI CCGCTC 1 cut(s) 70
MboI GATC 1 cut(s) 721
MboII GAAGA 1 cut(s) 72
MluCI AATT 2 cut(s) 98, 957
MlyI GAGTC 2 cut(s) 62, 362
MmeI TCCRAC 3 cut(s) 54, 657, 1007
Mph1103I ATGCAT 1 cut(s) 1027
MroXI GAANNNNTTC 1 cut(s) 578
MseI TTAA 2 cut(s) 117, 975
MslI CAYNNNNRTG 1 cut(s) 1030
MspR9I CCNGG 3 cut(s) 178, 251, 816
MvaI CCWGG 3 cut(s) 178, 251, 816
MvnI CGCG 1 cut(s) 293
MwoI GCNNNNNNNGC 4 cut(s) 313, 461, 583, 882
NcoI CCATGG 1 cut(s) 449
NdeII GATC 1 cut(s) 721
NlaIII CATG 7 cut(s) 316, 453, 496, 659, 844, 1007, 1029
NmeAIII GCCGAG 1 cut(s) 395
NmuCI GTSAC 3 cut(s) 434, 562, 623
NsbI TGCGCA 1 cut(s) 381
NsiI ATGCAT 1 cut(s) 1027
NspI RCATGY 3 cut(s) 316, 496, 844
PaeI GCATGC 1 cut(s) 316
PciI ACATGT 1 cut(s) 492
PdmI GAANNNNTTC 1 cut(s) 578
PfeI GAWTC 4 cut(s) 188, 547, 823, 1010
PfoI TCCNGGA 1 cut(s) 176
PkrI GCNGC 4 cut(s) 309, 384, 387, 473
PleI GAGTC 2 cut(s) 61, 361
PmaCI CACGTG 1 cut(s) 304
PmlI CACGTG 1 cut(s) 304
PpsI GAGTC 2 cut(s) 61, 361
Ppu21I YACGTR 1 cut(s) 304
PscI ACATGT 1 cut(s) 492
Psp6I CCWGG 3 cut(s) 176, 249, 814
PspCI CACGTG 1 cut(s) 304
PspFI CCCAGC 1 cut(s) 425
PspGI CCWGG 3 cut(s) 176, 249, 814
PspPI GGNCC 6 cut(s) 162, 180, 453, 463, 535, 785
PstNI CAGNNNCTG 1 cut(s) 1019
RsaI GTAC 3 cut(s) 413, 617, 805
RsaNI GTAC 3 cut(s) 412, 616, 804
RseI CAYNNNNRTG 1 cut(s) 1030
SaqAI TTAA 2 cut(s) 117, 975
SatI GCNGC 4 cut(s) 308, 383, 386, 472
Sau3AI GATC 1 cut(s) 721
Sau96I GGNCC 6 cut(s) 162, 180, 453, 463, 535, 785
ScaI AGTACT 1 cut(s) 413
SchI GAGTC 2 cut(s) 62, 362
ScrFI CCNGG 3 cut(s) 178, 251, 816
SfaNI GCATC 4 cut(s) 247, 472, 616, 1012
SfiI GGCCNNNNNGGCC 1 cut(s) 461
SinI GGWCC 3 cut(s) 180, 535, 785
SmiMI CAYNNNNRTG 1 cut(s) 1030
SphI GCATGC 1 cut(s) 316
Sse9I AATT 2 cut(s) 98, 957
SsiI CCGC 3 cut(s) 70, 165, 242
SspMI CTAG 2 cut(s) 47, 762
StyD4I CCNGG 3 cut(s) 176, 249, 814
StyI CCWWGG 4 cut(s) 270, 449, 466, 707
TaaI ACNGT 4 cut(s) 785, 803, 808, 1000
TaiI ACGT 3 cut(s) 306, 677, 858
TaqI TCGA 3 cut(s) 362, 610, 720
TaqII GACCGA 1 cut(s) 197
TasI AATT 2 cut(s) 98, 957
TatI WGTACW 3 cut(s) 411, 615, 803
TfiI GAWTC 4 cut(s) 188, 547, 823, 1010
Tru1I TTAA 2 cut(s) 117, 975
Tru9I TTAA 2 cut(s) 117, 975
TscAI CASTG 7 cut(s) 164, 205, 463, 667, 806, 993, 1005
TseFI GTSAC 3 cut(s) 434, 562, 623
TseI GCWGC 4 cut(s) 307, 382, 385, 471
Tsp45I GTSAC 3 cut(s) 434, 562, 623
TspDTI ATGAA 3 cut(s) 518, 839, 950
TspGWI ACGGA 1 cut(s) 611
TspRI CASTG 7 cut(s) 164, 205, 463, 667, 806, 993, 1005
VpaK11BI GGWCC 3 cut(s) 180, 535, 785
XceI RCATGY 3 cut(s) 316, 496, 844
XmiI GTMKAC 1 cut(s) 515
XmnI GAANNNNTTC 1 cut(s) 578
XspI CTAG 2 cut(s) 47, 762
ZrmI AGTACT 1 cut(s) 413
Zsp2I ATGCAT 1 cut(s) 1027
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.