Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr05

Orthogroup ID

OG0007727

Physical Location & Seq

Genomic Coordinates Forward (+)

37686510 .. 37691550

Gene Structure 5041 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD05G1244500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1914 bp

ATGGCTTTTCTGCCAAAGCTCACAGCTTTTTTTGTGGTGTTGTTATCAGTTTTTGAGAACCATGTGCATGCAAGGTTTGTGGTGGAGACGGAAAGCATTAAGGTTCTCTATCCAATGAGCTTGCGCTCGAAGCACGACGGAGCTATCGGCAATTTTGGGCGGCCGAACTATGGAGGCTCCCTGGTGGGCGCTGTTGTCTATCCTGAAAAGGGGTCTTCCGGGTGTGAAGCTTTTGAGGGTGACAAGCCTTTCAAGTCGAGACACTGTCGCTCCACCATTGTTTTACTTGATCGCGGAGGTTGCTACTTTGCTTTGAAGGTGTGGAATGCTCAACAGGCGGGAGCTAAAGCCGTTTTAGTGGCGGATAACATTGAAGAGCCTTTAATAACAATGGATTCCCCGGAAGAGAGCACAGATGCAGATGGTTACATAGAGAAGATTGGAATTCCATCAGCTCTGGTAGAGAAAGCTTTTGGTGATAGCTTGAAGGAAGCGTTAAAGAAGAAAGAAGATGTTGTGGTGAAACTTGACTGGAGAGAGTCCGTGCCAAACCCCGATCAAAGAGTTGAATACGAGTTCTGGACAAACAGCAACGACGAGTGTGGGGCTCGTTGTGATGAGCAAATGACATTTGTAAAAAATTTCAAGGGTCACGCTCAGGTTCTTGAGAGAGGGGGTTACACCCTCTTCACTCCACATTATATAACTTGGTATTGTCCTCGGGCTTTCATCCTTACAAAACAGTGCAAGTCTCAATGCATAAATCATGGGAGGTATTGTGCACCGGATCCTGAAAAAGATTTGGGAGAAGGGTACCAAGGGAAGGACGTGGTATACGAGAACTTGAGGCAGCTTTGTGTGCATAGAGTTGCCAATGAGAGCGGCCGGTCATGGGTTTGGTGGGACTATGTGACAGACTTCCATATCAGATGTTCAATGAAAGAAAAGAAGTATAGCAAAGAATGCGCTGAGGATGTCATGAAATCACTTGATTTGCCTGTTGACAAGATAAAAAAATGTATGGGTAATCCTGAAGATGATGCCGAGAATGCAGTGCTAAAAGCCGAACAAGAAATCCAGGTTGGACGAGGATCTCGTGGTGATGTTACCATCTTGCCGACTTTGGTTATTAACAATGTTCAGTTTCGAGGAAAATTAGAGAGAACTACTGTGCTAAAGGCCTTATGTGCTGGATTTAAGGAGATGGCTGATCCTCCTATTTGTTTGACTGGAGACCTCGAGACAAATGAGTGCGTTGAAAGGAATGGCGGTTGTTGGCAGGACATCCACTCTAATATAACTGCTTGCAAGGACACATTTAGGGGAAGAGTTTGTGAGTGCCCTATTGTGAACGGTGTTCAGTATAGAGGCGATGGTTATACATCTTGCGATGCTTTTGGACCTGCAAGATGTGCTATAAGCAATGGAGGTTGCTGGTCAGAATCTAAAAATGGATTGCATTTCTCAGCTTGCTCAAATTCTGAGTTATCTGGCTGCAAATGTCCACAAGGCTTTCAAGGGGACGGTCATAAATGTGAAGATATTGATGAATGCAAGGCGCATACTGCTTGTCAGTGTGATGGTTGCAGCTGTAAGAACACTTGGGGCGGGTACAACTGCAAATGTAAGGCAGGCCAGTTGTATATGAAGGAGCAAGATACTTGTATTGAGAGACATGGATCAAGATTTGCCTGGTTTTTAACTTTCATGGTCCTTGCGGCTGTGTCGGGAGTTGGCCTAGCCGGTTACATCTTCTACAAATACAGGCTTCGATCTTACATGGACTCTGAGATCACGGCTATCATGTCGCAATACATGCCTCTGGACAGTCAGCAGGCCAGTCAAGTGCACACCGCCGACTCAGAACCTCAACGACAAGGCTCAAAACACAAGCTAAACCACCTATTCCAGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PA cEGF

Protein Analysis

638

Amino Acids

71.2

Weight (kDa)

6.14

Isoelectric Point (pI)

41.03

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PA	PF02225	63 - 162	3.3e-17	PA domain
VSR_TRX	PF25011	189 - 396	1.2e-102	Vacuolar sorting receptor thioredoxin-like domain
cEGF	PF12662	499 - 517	1.4e-06	Complement Clr-like EGF-like

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0007727)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G30900 AT2G34940 AT4G20110 AT4G20110
fragaria_vesca	FvH4_3g13190 FvH4_3g13190
malus_domestica	MD05G1244500.v1.1 MD10G1226100.v1.1
prunus_persica	Prupe.4G119200_v2.0.a1 Prupe.4G119200_v2.0.a1 Prupe.4G119200_v2.0.a1
pyrus_communis	pycom05g22100 pycom10g19000
rosa_chinensis	RchiOBHm_Chr5g0021401
rosa_laevigata	RLG00000032610
rosa_multiflora	Rmu_co8489967.1_g000001 Rmu_sc0004847.1_g000021
rosa_roxburghii	Rroxscaffold_1G00057140
rosa_rugosa	Rorug05G0067000
rosa_samantha	Rh5AG156100 Rh5BG155600 Rh5CG169300 Rh5DG156100
rosa_wichuraiana	Rw5G013900

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	1411
Acc65I	GGTACC	1 cut(s)	813
AccB1I	GGYRCC	1 cut(s)	813
AccBSI	CCGCTC	1 cut(s)	882
AccI	GTMKAC	1 cut(s)	834
AccII	CGCG	1 cut(s)	294
AciI	CCGC	9 cut(s)	160, 294, 338, 362, 882, 1269, 1608, 1718, 1854
AclWI	GGATC	5 cut(s)	782, 795, 1099, 1205, 1687
AcoI	YGGCCR	2 cut(s)	161, 883
AcsI	RAATTY	3 cut(s)	444, 640, 1477
AcuI	CTGAAG	1 cut(s)	1053
AfaI	GTAC	2 cut(s)	815, 1613
AfiI	CCNNNNNNNGG	4 cut(s)	170, 209, 823, 892
AgsI	TTSAA	9 cut(s)	253, 316, 374, 487, 569, 646, 936, 1259, 1517
AjiI	CACGTC	1 cut(s)	829
AjnI	CCWGG	3 cut(s)	180, 1077, 1691
AjuI	GAANNNNNNNTTGG	2 cut(s)	1065, 1097
AloI	GAACNNNNNNTCC	2 cut(s)	1059, 1091
Alw21I	GWGCWC	3 cut(s)	413, 784, 1851
Alw26I	GTCTC	6 cut(s)	80, 253, 756, 1227, 1235, 1666
Alw44I	GTGCAC	2 cut(s)	780, 1847
AlwI	GGATC	5 cut(s)	782, 795, 1099, 1205, 1687
Ama87I	CYCGRG	2 cut(s)	720, 1238
AoxI	GGCC	6 cut(s)	161, 883, 1179, 1633, 1735, 1836
ApaLI	GTGCAC	2 cut(s)	780, 1847
ApeKI	GCWGC	3 cut(s)	850, 1494, 1587
ApoI	RAATTY	3 cut(s)	444, 640, 1477
Asp718I	GGTACC	1 cut(s)	813
AspLEI	GCGC	4 cut(s)	126, 191, 968, 1561
AspS9I	GGNCC	2 cut(s)	1400, 1711
AsuC2I	CCSGG	2 cut(s)	220, 401
AsuHPI	GGTGA	4 cut(s)	251, 488, 532, 1112
AvaI	CYCGRG	2 cut(s)	720, 1238
AvaII	GGWCC	2 cut(s)	1400, 1711
BaeGI	GKGCMC	3 cut(s)	784, 1343, 1851
BamHI	GGATCC	1 cut(s)	787
BanI	GGYRCC	1 cut(s)	813
BanII	GRGCYC	1 cut(s)	610
BauI	CACGAG	1 cut(s)	1095
BbsI	GAAGAC	1 cut(s)	207
Bbv12I	GWGCWC	3 cut(s)	413, 784, 1851
BbvCI	CCTCAGC	1 cut(s)	969
BbvI	GCAGC	3 cut(s)	862, 1481, 1599
BccI	CCATC	6 cut(s)	416, 457, 1118, 1198, 1367, 1574
BceAI	ACGGC	2 cut(s)	335, 1812
BciT130I	CCWGG	3 cut(s)	182, 1079, 1693
BcnI	CCSGG	2 cut(s)	220, 401
BcoDI	GTCTC	6 cut(s)	80, 253, 756, 1227, 1235, 1666
BfaI	CTAG	1 cut(s)	1739
BfoI	RGCGCY	1 cut(s)	192
BfuAI	ACCTGC	1 cut(s)	1411
BisI	GCNGC	6 cut(s)	161, 851, 883, 1495, 1588, 1719
BlsI	GCNGC	6 cut(s)	162, 852, 884, 1496, 1589, 1720
Bme1390I	CCNGG	5 cut(s)	182, 220, 401, 1079, 1693
Bme18I	GGWCC	2 cut(s)	1400, 1711
BmeT110I	CYCGRG	2 cut(s)	720, 1238
BmgBI	CACGTC	1 cut(s)	829
BmgT120I	GGNCC	2 cut(s)	1400, 1711
BmiI	GGNNCC	3 cut(s)	178, 789, 815
BmrFI	CCNGG	5 cut(s)	182, 220, 401, 1079, 1693
BmsI	GCATC	3 cut(s)	406, 1030, 1381
BpiI	GAAGAC	1 cut(s)	207
BpmI	CTGGAG	2 cut(s)	553, 1251
Bpu10I	CCTNAGC	2 cut(s)	657, 969
BpuEI	CTTGAG	2 cut(s)	686, 865
BpuMI	CCSGG	2 cut(s)	220, 401
BsaI	GGTCTC	1 cut(s)	1227
BsaJI	CCNNGG	4 cut(s)	180, 399, 719, 817
BsaWI	WCCGGW	1 cut(s)	784
BsaXI	ACNNNNNCTCC	4 cut(s)	254, 284, 763, 793
Bsc4I	CCNNNNNNNGG	4 cut(s)	170, 209, 823, 892
Bse118I	RCCGGY	2 cut(s)	885, 1742
Bse1I	ACTGG	5 cut(s)	536, 1234, 1636, 1839, 1909
Bse3DI	GCAATG	1 cut(s)	1429
BseBI	CCWGG	3 cut(s)	182, 1079, 1693
BseDI	CCNNGG	4 cut(s)	180, 399, 719, 817
BseGI	GGATG	3 cut(s)	729, 979, 1284
BseLI	CCNNNNNNNGG	4 cut(s)	170, 209, 823, 892
BseMI	GCAATG	1 cut(s)	1429
BseMII	CTCAG	6 cut(s)	671, 960, 1473, 1479, 1779, 1875
BseNI	ACTGG	5 cut(s)	536, 1234, 1636, 1839, 1909
BseSI	GKGCMC	3 cut(s)	784, 1343, 1851
BseX3I	CGGCCG	2 cut(s)	161, 883
BseXI	GCAGC	3 cut(s)	862, 1481, 1599
Bsh1236I	CGCG	1 cut(s)	294
Bsh1285I	CGRYCG	2 cut(s)	164, 886
BshFI	GGCC	6 cut(s)	163, 885, 1181, 1635, 1737, 1838
BshNI	GGYRCC	1 cut(s)	813
BsiEI	CGRYCG	2 cut(s)	164, 886
BsiHKAI	GWGCWC	3 cut(s)	413, 784, 1851
BsiHKCI	CYCGRG	2 cut(s)	720, 1238
BsiSI	CCGG	5 cut(s)	219, 401, 785, 886, 1743
BslFI	GGGAC	2 cut(s)	917, 1535
BslI	CCNNNNNNNGG	4 cut(s)	170, 209, 823, 892
BsmAI	GTCTC	6 cut(s)	80, 253, 756, 1227, 1235, 1666
BsmBI	CGTCTC	1 cut(s)	80
BsmFI	GGGAC	2 cut(s)	917, 1535
BsmI	GAATGC	4 cut(s)	331, 968, 1054, 1556
BsnI	GGCC	6 cut(s)	163, 885, 1181, 1635, 1737, 1838
Bso31I	GGTCTC	1 cut(s)	1227
BsoBI	CYCGRG	2 cut(s)	720, 1238
Bsp1286I	GDGCHC	5 cut(s)	413, 610, 784, 1343, 1851
Bsp143I	GATC	8 cut(s)	289, 556, 787, 1091, 1210, 1679, 1772, 1791
BspACI	CCGC	9 cut(s)	160, 294, 338, 362, 882, 1269, 1608, 1718, 1854
BspANI	GGCC	6 cut(s)	163, 885, 1181, 1635, 1737, 1838
BspCNI	CTCAG	6 cut(s)	670, 961, 1474, 1478, 1780, 1874
BspFNI	CGCG	1 cut(s)	294
BspHI	TCATGA	1 cut(s)	978
BspLI	GGNNCC	3 cut(s)	178, 789, 815
BspMI	ACCTGC	1 cut(s)	1411
BspPI	GGATC	5 cut(s)	782, 795, 1099, 1205, 1687
BspQI	GCTCTTC	1 cut(s)	369
BspT107I	GGYRCC	1 cut(s)	813
BspTNI	GGTCTC	1 cut(s)	1227
BsrBI	CCGCTC	1 cut(s)	882
BsrDI	GCAATG	1 cut(s)	1429
BsrFI	RCCGGY	2 cut(s)	885, 1742
BsrI	ACTGG	5 cut(s)	536, 1234, 1636, 1839, 1909
BssAI	RCCGGY	2 cut(s)	885, 1742
BssECI	CCNNGG	4 cut(s)	180, 399, 719, 817
BssMI	GATC	8 cut(s)	289, 556, 787, 1091, 1210, 1679, 1772, 1791
BssNAI	GTATAC	1 cut(s)	835
BssSI	CACGAG	1 cut(s)	1095
BssT1I	CCWWGG	1 cut(s)	817
Bst1107I	GTATAC	1 cut(s)	835
Bst2BI	CACGAG	1 cut(s)	1095
Bst2UI	CCWGG	3 cut(s)	182, 1079, 1693
Bst4CI	ACNGT	6 cut(s)	266, 744, 1171, 1355, 1526, 1829
Bst6I	CTCTTC	4 cut(s)	369, 399, 692, 1321
BstAPI	GCANNNNNTGC	3 cut(s)	963, 1412, 1816
BstC8I	GCNNGC	6 cut(s)	69, 122, 1306, 1471, 1633, 1836
BstDEI	CTNAG	6 cut(s)	657, 969, 1465, 1482, 1788, 1861
BstF5I	GGATG	3 cut(s)	729, 979, 1284
BstFNI	CGCG	1 cut(s)	294
BstH2I	RGCGCY	1 cut(s)	192
BstHHI	GCGC	4 cut(s)	126, 191, 968, 1561
BstKTI	GATC	8 cut(s)	292, 559, 790, 1094, 1213, 1682, 1775, 1794
BstMAI	GTCTC	6 cut(s)	80, 253, 756, 1227, 1235, 1666
BstMBI	GATC	8 cut(s)	289, 556, 787, 1091, 1210, 1679, 1772, 1791
BstMCI	CGRYCG	2 cut(s)	164, 886
BstNI	CCWGG	3 cut(s)	182, 1079, 1693
BstNSI	RCATGY	2 cut(s)	71, 1819
BstSCI	CCNGG	5 cut(s)	180, 218, 399, 1077, 1691
BstSLI	GKGCMC	3 cut(s)	784, 1343, 1851
BstUI	CGCG	1 cut(s)	294
BstV1I	GCAGC	3 cut(s)	862, 1481, 1599
BstV2I	GAAGAC	1 cut(s)	207
BstX2I	RGATCY	2 cut(s)	787, 1091
BstYI	RGATCY	2 cut(s)	787, 1091
BstZ17I	GTATAC	1 cut(s)	835
BstZI	CGGCCG	2 cut(s)	161, 883
BsuRI	GGCC	6 cut(s)	163, 885, 1181, 1635, 1737, 1838
BtgZI	GCGATG	2 cut(s)	1386, 1404
BtrI	CACGTC	1 cut(s)	829
BtsCI	GGATG	3 cut(s)	729, 979, 1284
BtsI	GCAGTG	1 cut(s)	1059
BtsIMutI	CAGTG	4 cut(s)	262, 749, 1059, 1580
BveI	ACCTGC	1 cut(s)	1411
Cac8I	GCNNGC	6 cut(s)	69, 122, 1306, 1471, 1633, 1836
CciI	TCATGA	1 cut(s)	978
CfoI	GCGC	4 cut(s)	126, 191, 968, 1561
Cfr10I	RCCGGY	2 cut(s)	885, 1742
Cfr13I	GGNCC	2 cut(s)	1400, 1711
Csp6I	GTAC	2 cut(s)	814, 1612
CspCI	CAANNNNNGTGG	2 cut(s)	60, 95
CviQI	GTAC	2 cut(s)	814, 1612
DdeI	CTNAG	6 cut(s)	657, 969, 1465, 1482, 1788, 1861
DpnI	GATC	8 cut(s)	291, 558, 789, 1093, 1212, 1681, 1774, 1793
DpnII	GATC	8 cut(s)	289, 556, 787, 1091, 1210, 1679, 1772, 1791
EaeI	YGGCCR	2 cut(s)	161, 883
EagI	CGGCCG	2 cut(s)	161, 883
Eam1104I	CTCTTC	4 cut(s)	369, 399, 692, 1321
EarI	CTCTTC	4 cut(s)	369, 399, 692, 1321
EciI	GGCGGA	1 cut(s)	377
EclXI	CGGCCG	2 cut(s)	161, 883
Eco130I	CCWWGG	1 cut(s)	817
Eco147I	AGGCCT	1 cut(s)	1181
Eco24I	GRGCYC	1 cut(s)	610
Eco31I	GGTCTC	1 cut(s)	1227
Eco47I	GGWCC	2 cut(s)	1400, 1711
Eco52I	CGGCCG	2 cut(s)	161, 883
Eco57I	CTGAAG	1 cut(s)	1053
Eco88I	CYCGRG	2 cut(s)	720, 1238
EcoRI	GAATTC	1 cut(s)	444
EcoRII	CCWGG	3 cut(s)	180, 1077, 1691
EcoT14I	CCWWGG	1 cut(s)	817
EcoT22I	ATGCAT	1 cut(s)	761
EcoT38I	GRGCYC	1 cut(s)	610
ErhI	CCWWGG	1 cut(s)	817
Esp3I	CGTCTC	1 cut(s)	80
FaqI	GGGAC	2 cut(s)	917, 1535
FauI	CCCGC	2 cut(s)	331, 1601
FblI	GTMKAC	1 cut(s)	834
Fnu4HI	GCNGC	6 cut(s)	161, 851, 883, 1495, 1588, 1719
FokI	GGATG	3 cut(s)	716, 986, 1271
FriOI	GRGCYC	1 cut(s)	610
Fsp4HI	GCNGC	6 cut(s)	161, 851, 883, 1495, 1588, 1719
FspBI	CTAG	1 cut(s)	1739
GlaI	GCGC	4 cut(s)	125, 190, 967, 1560
GluI	GCNGC	6 cut(s)	161, 851, 883, 1495, 1588, 1719
GsuI	CTGGAG	2 cut(s)	553, 1251
HaeII	RGCGCY	1 cut(s)	192
HaeIII	GGCC	6 cut(s)	163, 885, 1181, 1635, 1737, 1838
HapII	CCGG	5 cut(s)	219, 401, 785, 886, 1743
HhaI	GCGC	4 cut(s)	126, 191, 968, 1561
Hin6I	GCGC	4 cut(s)	124, 189, 966, 1559
HinP1I	GCGC	4 cut(s)	124, 189, 966, 1559
HincII	GTYRAC	1 cut(s)	1003
HindII	GTYRAC	1 cut(s)	1003
HindIII	AAGCTT	2 cut(s)	228, 468
HinfI	GANTC	5 cut(s)	395, 539, 1442, 1784, 1859
HpaII	CCGG	5 cut(s)	219, 401, 785, 886, 1743
HphI	GGTGA	4 cut(s)	251, 488, 532, 1112
Hpy166II	GTNNAC	6 cut(s)	782, 835, 1003, 1351, 1505, 1849
Hpy188I	TCNGA	5 cut(s)	929, 1441, 1483, 1789, 1864
Hpy8I	GTNNAC	6 cut(s)	782, 835, 1003, 1351, 1505, 1849
Hpy99I	CGWCG	2 cut(s)	140, 599
HpyAV	CCTTC	5 cut(s)	310, 481, 803, 817, 1642
HpyCH4III	ACNGT	6 cut(s)	266, 744, 1171, 1355, 1526, 1829
HpyCH4IV	ACGT	1 cut(s)	828
HpyF3I	CTNAG	6 cut(s)	657, 969, 1465, 1482, 1788, 1861
HpySE526I	ACGT	1 cut(s)	828
HspAI	GCGC	4 cut(s)	124, 189, 966, 1559
KpnI	GGTACC	1 cut(s)	817
Kzo9I	GATC	8 cut(s)	289, 556, 787, 1091, 1210, 1679, 1772, 1791
LguI	GCTCTTC	1 cut(s)	369
LmnI	GCTCC	5 cut(s)	140, 182, 275, 341, 1651
Lsp1109I	GCAGC	3 cut(s)	862, 1481, 1599
LweI	GCATC	3 cut(s)	406, 1030, 1381
MaeI	CTAG	1 cut(s)	1739
MaeII	ACGT	1 cut(s)	828
MaeIII	GTNAC	7 cut(s)	239, 425, 650, 677, 910, 1105, 1745
MalI	GATC	8 cut(s)	291, 558, 789, 1093, 1212, 1681, 1774, 1793
MbiI	CCGCTC	1 cut(s)	882
MboI	GATC	8 cut(s)	289, 556, 787, 1091, 1210, 1679, 1772, 1791
MflI	RGATCY	2 cut(s)	787, 1091
MhlI	GDGCHC	5 cut(s)	413, 610, 784, 1343, 1851
MluCI	AATT	5 cut(s)	151, 444, 640, 1154, 1477
MlyI	GAGTC	3 cut(s)	548, 1778, 1853
MmeI	TCCRAC	1 cut(s)	1063
Mph1103I	ATGCAT	1 cut(s)	761
MseI	TTAA	6 cut(s)	99, 383, 497, 1131, 1197, 1700
MslI	CAYNNNNRTG	2 cut(s)	66, 1533
MspA1I	CMGCKG	1 cut(s)	1590
MspI	CCGG	5 cut(s)	219, 401, 785, 886, 1743
MspR9I	CCNGG	5 cut(s)	182, 220, 401, 1079, 1693
Mva1269I	GAATGC	4 cut(s)	331, 968, 1054, 1556
MvaI	CCWGG	3 cut(s)	182, 1079, 1693
MvnI	CGCG	1 cut(s)	294
NciI	CCSGG	2 cut(s)	220, 401
NdeII	GATC	8 cut(s)	289, 556, 787, 1091, 1210, 1679, 1772, 1791
NlaIV	GGNNCC	3 cut(s)	178, 789, 815
NmeAIII	GCCGAG	1 cut(s)	1069
NmuCI	GTSAC	3 cut(s)	239, 650, 910
NsiI	ATGCAT	1 cut(s)	761
NspI	RCATGY	2 cut(s)	71, 1819
PaeI	GCATGC	1 cut(s)	71
PaeR7I	CTCGAG	1 cut(s)	1238
PagI	TCATGA	1 cut(s)	978
PceI	AGGCCT	1 cut(s)	1181
PciSI	GCTCTTC	1 cut(s)	369
PcsI	WCGNNNNNNNCGW	2 cut(s)	834, 1093
PctI	GAATGC	4 cut(s)	331, 968, 1054, 1556
PfeI	GAWTC	2 cut(s)	395, 1442
PflFI	GACNNNGTC	1 cut(s)	264
PkrI	GCNGC	6 cut(s)	162, 852, 884, 1496, 1589, 1720
PleI	GAGTC	3 cut(s)	547, 1778, 1853
PpsI	GAGTC	3 cut(s)	547, 1778, 1853
Psp6I	CCWGG	3 cut(s)	180, 1077, 1691
PspGI	CCWGG	3 cut(s)	180, 1077, 1691
PspN4I	GGNNCC	3 cut(s)	178, 789, 815
PspPI	GGNCC	2 cut(s)	1400, 1711
PsuI	RGATCY	2 cut(s)	787, 1091
PsyI	GACNNNGTC	1 cut(s)	264
PvuII	CAGCTG	1 cut(s)	1590
RsaI	GTAC	2 cut(s)	815, 1613
RsaNI	GTAC	2 cut(s)	814, 1612
RseI	CAYNNNNRTG	2 cut(s)	66, 1533
SapI	GCTCTTC	1 cut(s)	369
SaqAI	TTAA	6 cut(s)	99, 383, 497, 1131, 1197, 1700
SatI	GCNGC	6 cut(s)	161, 851, 883, 1495, 1588, 1719
Sau3AI	GATC	8 cut(s)	289, 556, 787, 1091, 1210, 1679, 1772, 1791
Sau96I	GGNCC	2 cut(s)	1400, 1711
SchI	GAGTC	3 cut(s)	548, 1778, 1853
ScrFI	CCNGG	5 cut(s)	182, 220, 401, 1079, 1693
SduI	GDGCHC	5 cut(s)	413, 610, 784, 1343, 1851
SfaNI	GCATC	3 cut(s)	406, 1030, 1381
Sfr274I	CTCGAG	1 cut(s)	1238
SinI	GGWCC	2 cut(s)	1400, 1711
SlaI	CTCGAG	1 cut(s)	1238
SmiMI	CAYNNNNRTG	2 cut(s)	66, 1533
SmlI	CTYRAG	3 cut(s)	665, 844, 1238
SmoI	CTYRAG	3 cut(s)	665, 844, 1238
SphI	GCATGC	1 cut(s)	71
Sse9I	AATT	5 cut(s)	151, 444, 640, 1154, 1477
SseBI	AGGCCT	1 cut(s)	1181
SsiI	CCGC	9 cut(s)	160, 294, 338, 362, 882, 1269, 1608, 1718, 1854
SspMI	CTAG	1 cut(s)	1739
StuI	AGGCCT	1 cut(s)	1181
StyD4I	CCNGG	5 cut(s)	180, 218, 399, 1077, 1691
StyI	CCWWGG	1 cut(s)	817
TaaI	ACNGT	6 cut(s)	266, 744, 1171, 1355, 1526, 1829
TaiI	ACGT	1 cut(s)	831
TaqI	TCGA	5 cut(s)	128, 257, 1147, 1239, 1771
TasI	AATT	5 cut(s)	151, 444, 640, 1154, 1477
TauI	GCSGC	3 cut(s)	163, 885, 1721
TfiI	GAWTC	2 cut(s)	395, 1442
Tru1I	TTAA	6 cut(s)	99, 383, 497, 1131, 1197, 1700
Tru9I	TTAA	6 cut(s)	99, 383, 497, 1131, 1197, 1700
TscAI	CASTG	4 cut(s)	269, 749, 1059, 1580
TseFI	GTSAC	3 cut(s)	239, 650, 910
TseI	GCWGC	3 cut(s)	850, 1494, 1587
Tsp45I	GTSAC	3 cut(s)	239, 650, 910
TspDTI	ATGAA	6 cut(s)	718, 953, 995, 1563, 1661, 1696
TspGWI	ACGGA	3 cut(s)	104, 153, 532
TspRI	CASTG	4 cut(s)	269, 749, 1059, 1580
Tth111I	GACNNNGTC	1 cut(s)	264
VneI	GTGCAC	2 cut(s)	780, 1847
VpaK11BI	GGWCC	2 cut(s)	1400, 1711
XapI	RAATTY	3 cut(s)	444, 640, 1477
XceI	RCATGY	2 cut(s)	71, 1819
XhoI	CTCGAG	1 cut(s)	1238
XmiI	GTMKAC	1 cut(s)	834
XspI	CTAG	1 cut(s)	1739
Zsp2I	ATGCAT	1 cut(s)	761

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.