pycom05g22100

Vacuolar-sorting receptor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0007727
Physical Location & Seq
Forward (+)
24608622 .. 24610933
2312 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g22100.4

Sequence Viewer

Length: 987 bp
ATGAAAGAAAAGAAGTATAGCAAAGAATGCGCTGAGGATGTCATGAAATCACTTGATTTGCCCGTTGACAAGATAAAAAAATGTATGGGTAATCCTGAAGATGATATCGAGAATGCAGTGCTAAAAGCCGAACAAGAAATCCAGTTAGGTTGGACGAGGATCGATCGTGGTGATATTGCCATCTTGCCGACTTTGGTTATTAACAATGTTCAGTTTCGAGGAAAATTAGAGAGAACTACTGTGCTAAAGGCCATATGTGCTGGATTTAAGGAGACGGCTGATCCTCCTATTTGTTTGACTGGAGACCTCGAGACAAATGAGTGCCTTGGAAGGAATGGCGGTTGTTGGCAGGACATCCAATCTAATATAACTGCTTGCAAGGACACATTTAGGGGAAGAGTTTGTGAGTGCCCTCTTGTGAACGGTGTTCAGTATAAAGGCGATGGTTATATATCTTGCCATGCTTTTGGACCTGCAAGATGTGCTATAAACAATGGAGGTTGCTGGTCAGAATCTAAAAATGGATTGCATTTCTCAGCTTGCTCAAATTCTGAGTTATCTGGCTGCAAATGTCCACAAGGCTTTCAAGGGGACGGTCATAAATGCGAAGATATTGATGAATGCAAGGCGAAAACCGCTTGTCAGTGTGATGGTTGCAGCTGTAAGAACACTTGGGGCGGGTACAACTGCAAATGTAAGGGAGGCCAGTTGTATATGAAGGAGCAAGATACTTGTATTGAGAGACATGGATCAAGATTTGCCTGGTTTTTAACTTTCATGGTCCTTGCGGCTGTGTCGGGAGTTGGCCTAGCCGGTTACATCTTCTACAAATACAGGCTTCGATCTTACATGGACTCTGAGATCACGGCTATCATGTCGCAATACATGCCTCTGGACAGTCAGCAGGCCAGTCAAGTGCACACCGCCGACTCAGAACCTCAACGACAAGGCTCGGTAAAACACAAGCTAAACCACCTATTCCAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PA cEGF

Protein Analysis

329

Amino Acids

36.35

Weight (kDa)

6.64

Isoelectric Point (pI)

53.33

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 481
AciI CCGC 5 cut(s) 339, 636, 678, 788, 924
AclWI GGATC 3 cut(s) 167, 275, 757
AcsI RAATTY 1 cut(s) 547
AcuI CTGAAG 1 cut(s) 117
AfaI GTAC 1 cut(s) 683
AgsI TTSAA 1 cut(s) 587
AjnI CCWGG 1 cut(s) 761
AloI GAACNNNNNNTCC 2 cut(s) 123, 155
AluBI AGCT 3 cut(s) 539, 660, 967
AluI AGCT 3 cut(s) 539, 660, 967
Alw21I GWGCWC 1 cut(s) 921
Alw26I GTCTC 4 cut(s) 266, 297, 305, 736
Alw44I GTGCAC 1 cut(s) 917
AlwI GGATC 3 cut(s) 167, 275, 757
Ama87I CYCGRG 1 cut(s) 308
AoxI GGCC 4 cut(s) 249, 703, 805, 906
ApaLI GTGCAC 1 cut(s) 917
ApeKI GCWGC 2 cut(s) 564, 657
ApoI RAATTY 1 cut(s) 547
AspLEI GCGC 1 cut(s) 32
AspS9I GGNCC 2 cut(s) 470, 781
AsuHPI GGTGA 1 cut(s) 182
AvaI CYCGRG 1 cut(s) 308
AvaII GGWCC 2 cut(s) 470, 781
BaeGI GKGCMC 2 cut(s) 413, 921
Bbv12I GWGCWC 1 cut(s) 921
BbvCI CCTCAGC 1 cut(s) 33
BbvI GCAGC 2 cut(s) 551, 669
BccI CCATC 3 cut(s) 188, 437, 644
BceAI ACGGC 2 cut(s) 291, 882
BciT130I CCWGG 1 cut(s) 763
BcoDI GTCTC 4 cut(s) 266, 297, 305, 736
BfaI CTAG 1 cut(s) 809
BfuAI ACCTGC 1 cut(s) 481
BisI GCNGC 3 cut(s) 565, 658, 789
BlsI GCNGC 3 cut(s) 566, 659, 790
Bme1390I CCNGG 1 cut(s) 763
Bme18I GGWCC 2 cut(s) 470, 781
BmeT110I CYCGRG 1 cut(s) 308
BmgT120I GGNCC 2 cut(s) 470, 781
BmrFI CCNGG 1 cut(s) 763
BpmI CTGGAG 1 cut(s) 321
Bpu10I CCTNAGC 1 cut(s) 33
Bsa29I ATCGAT 1 cut(s) 162
BsaI GGTCTC 1 cut(s) 297
BsaJI CCNNGG 1 cut(s) 325
Bse118I RCCGGY 1 cut(s) 812
Bse1I ACTGG 5 cut(s) 142, 304, 706, 909, 982
BseBI CCWGG 1 cut(s) 763
BseCI ATCGAT 1 cut(s) 162
BseDI CCNNGG 1 cut(s) 325
BseGI GGATG 2 cut(s) 43, 354
BseMII CTCAG 5 cut(s) 24, 543, 549, 849, 945
BseNI ACTGG 5 cut(s) 142, 304, 706, 909, 982
BseSI GKGCMC 2 cut(s) 413, 921
BseXI GCAGC 2 cut(s) 551, 669
Bsh1285I CGRYCG 1 cut(s) 166
BshFI GGCC 4 cut(s) 251, 705, 807, 908
BshVI ATCGAT 1 cut(s) 162
BsiEI CGRYCG 1 cut(s) 166
BsiHKAI GWGCWC 1 cut(s) 921
BsiHKCI CYCGRG 1 cut(s) 308
BsiSI CCGG 1 cut(s) 813
BslFI GGGAC 1 cut(s) 605
BsmAI GTCTC 4 cut(s) 266, 297, 305, 736
BsmBI CGTCTC 1 cut(s) 266
BsmFI GGGAC 1 cut(s) 605
BsmI GAATGC 3 cut(s) 32, 118, 626
BsnI GGCC 4 cut(s) 251, 705, 807, 908
Bso31I GGTCTC 1 cut(s) 297
BsoBI CYCGRG 1 cut(s) 308
Bsp1286I GDGCHC 2 cut(s) 413, 921
Bsp143I GATC 6 cut(s) 159, 163, 280, 749, 842, 861
BspACI CCGC 5 cut(s) 339, 636, 678, 788, 924
BspANI GGCC 4 cut(s) 251, 705, 807, 908
BspCNI CTCAG 5 cut(s) 25, 544, 548, 850, 944
BspDI ATCGAT 1 cut(s) 162
BspHI TCATGA 1 cut(s) 42
BspMI ACCTGC 1 cut(s) 481
BspPI GGATC 3 cut(s) 167, 275, 757
BspTNI GGTCTC 1 cut(s) 297
BsrFI RCCGGY 1 cut(s) 812
BsrI ACTGG 5 cut(s) 142, 304, 706, 909, 982
BssAI RCCGGY 1 cut(s) 812
BssECI CCNNGG 1 cut(s) 325
BssMI GATC 6 cut(s) 159, 163, 280, 749, 842, 861
BssT1I CCWWGG 1 cut(s) 325
Bst2UI CCWGG 1 cut(s) 763
Bst4CI ACNGT 4 cut(s) 241, 425, 596, 899
Bst6I CTCTTC 1 cut(s) 391
BstAPI GCANNNNNTGC 3 cut(s) 27, 482, 886
BstC8I GCNNGC 3 cut(s) 376, 541, 906
BstDEI CTNAG 5 cut(s) 33, 535, 552, 858, 931
BstF5I GGATG 2 cut(s) 43, 354
BstHHI GCGC 1 cut(s) 32
BstKTI GATC 6 cut(s) 162, 166, 283, 752, 845, 864
BstMAI GTCTC 4 cut(s) 266, 297, 305, 736
BstMBI GATC 6 cut(s) 159, 163, 280, 749, 842, 861
BstMCI CGRYCG 1 cut(s) 166
BstMWI GCNNNNNNNGC 5 cut(s) 27, 257, 482, 635, 886
BstNI CCWGG 1 cut(s) 763
BstNSI RCATGY 1 cut(s) 889
BstSCI CCNGG 1 cut(s) 761
BstSLI GKGCMC 2 cut(s) 413, 921
BstV1I GCAGC 2 cut(s) 551, 669
BstXI CCANNNNNNTGG 1 cut(s) 467
Bsu15I ATCGAT 1 cut(s) 162
BsuRI GGCC 4 cut(s) 251, 705, 807, 908
BsuTUI ATCGAT 1 cut(s) 162
BtgZI GCGATG 1 cut(s) 456
BtsCI GGATG 2 cut(s) 43, 354
BtsI GCAGTG 1 cut(s) 123
BtsIMutI CAGTG 2 cut(s) 123, 650
BveI ACCTGC 1 cut(s) 481
Cac8I GCNNGC 3 cut(s) 376, 541, 906
CciI TCATGA 1 cut(s) 42
CfoI GCGC 1 cut(s) 32
Cfr10I RCCGGY 1 cut(s) 812
Cfr13I GGNCC 2 cut(s) 470, 781
ClaI ATCGAT 1 cut(s) 162
Csp6I GTAC 1 cut(s) 682
CviAII CATG 7 cut(s) 43, 461, 746, 778, 850, 874, 886
CviQI GTAC 1 cut(s) 682
DdeI CTNAG 5 cut(s) 33, 535, 552, 858, 931
DpnI GATC 6 cut(s) 161, 165, 282, 751, 844, 863
DpnII GATC 6 cut(s) 159, 163, 280, 749, 842, 861
Eam1104I CTCTTC 1 cut(s) 391
EarI CTCTTC 1 cut(s) 391
Eco130I CCWWGG 1 cut(s) 325
Eco31I GGTCTC 1 cut(s) 297
Eco32I GATATC 1 cut(s) 106
Eco47I GGWCC 2 cut(s) 470, 781
Eco57I CTGAAG 1 cut(s) 117
Eco88I CYCGRG 1 cut(s) 308
EcoRII CCWGG 1 cut(s) 761
EcoRV GATATC 1 cut(s) 106
EcoT14I CCWWGG 1 cut(s) 325
ErhI CCWWGG 1 cut(s) 325
Esp3I CGTCTC 1 cut(s) 266
FaeI CATG 7 cut(s) 46, 464, 749, 781, 853, 877, 889
FaqI GGGAC 1 cut(s) 605
FatI CATG 7 cut(s) 42, 460, 745, 777, 849, 873, 885
FauI CCCGC 1 cut(s) 671
FauNDI CATATG 1 cut(s) 254
Fnu4HI GCNGC 3 cut(s) 565, 658, 789
FokI GGATG 2 cut(s) 50, 341
Fsp4HI GCNGC 3 cut(s) 565, 658, 789
FspBI CTAG 1 cut(s) 809
GlaI GCGC 1 cut(s) 31
GluI GCNGC 3 cut(s) 565, 658, 789
GsuI CTGGAG 1 cut(s) 321
HaeIII GGCC 4 cut(s) 251, 705, 807, 908
HapII CCGG 1 cut(s) 813
HhaI GCGC 1 cut(s) 32
Hin1II CATG 7 cut(s) 46, 464, 749, 781, 853, 877, 889
Hin6I GCGC 1 cut(s) 30
HinP1I GCGC 1 cut(s) 30
HincII GTYRAC 1 cut(s) 67
HindII GTYRAC 1 cut(s) 67
HinfI GANTC 3 cut(s) 512, 854, 929
HpaII CCGG 1 cut(s) 813
HphI GGTGA 1 cut(s) 182
Hpy166II GTNNAC 4 cut(s) 67, 421, 575, 919
Hpy188I TCNGA 4 cut(s) 511, 553, 859, 934
Hpy188III TCNNGA 7 cut(s) 43, 95, 109, 310, 753, 798, 893
Hpy8I GTNNAC 4 cut(s) 67, 421, 575, 919
HpyAV CCTTC 2 cut(s) 324, 712
HpyCH4III ACNGT 4 cut(s) 241, 425, 596, 899
HpyCH4V TGCA 9 cut(s) 116, 378, 476, 529, 567, 624, 657, 690, 919
HpyF10VI GCNNNNNNNGC 5 cut(s) 27, 257, 482, 635, 886
HpyF3I CTNAG 5 cut(s) 33, 535, 552, 858, 931
Hsp92II CATG 7 cut(s) 46, 464, 749, 781, 853, 877, 889
HspAI GCGC 1 cut(s) 30
Kzo9I GATC 6 cut(s) 159, 163, 280, 749, 842, 861
LmnI GCTCC 1 cut(s) 721
Lsp1109I GCAGC 2 cut(s) 551, 669
MaeI CTAG 1 cut(s) 809
MaeIII GTNAC 1 cut(s) 815
MalI GATC 6 cut(s) 161, 165, 282, 751, 844, 863
MboI GATC 6 cut(s) 159, 163, 280, 749, 842, 861
MboII GAAGA 4 cut(s) 110, 408, 620, 814
MhlI GDGCHC 2 cut(s) 413, 921
MluCI AATT 2 cut(s) 224, 547
MlyI GAGTC 2 cut(s) 848, 923
MmeI TCCRAC 1 cut(s) 131
MseI TTAA 3 cut(s) 201, 267, 770
MspA1I CMGCKG 1 cut(s) 660
MspI CCGG 1 cut(s) 813
MspR9I CCNGG 1 cut(s) 763
Mva1269I GAATGC 3 cut(s) 32, 118, 626
MvaI CCWGG 1 cut(s) 763
MwoI GCNNNNNNNGC 5 cut(s) 27, 257, 482, 635, 886
NdeI CATATG 1 cut(s) 254
NdeII GATC 6 cut(s) 159, 163, 280, 749, 842, 861
NlaIII CATG 7 cut(s) 46, 464, 749, 781, 853, 877, 889
NspI RCATGY 1 cut(s) 889
PaeR7I CTCGAG 1 cut(s) 308
PagI TCATGA 1 cut(s) 42
PctI GAATGC 3 cut(s) 32, 118, 626
PfeI GAWTC 1 cut(s) 512
PkrI GCNGC 3 cut(s) 566, 659, 790
Ple19I CGATCG 1 cut(s) 166
PleI GAGTC 2 cut(s) 848, 923
PpsI GAGTC 2 cut(s) 848, 923
Psp6I CCWGG 1 cut(s) 761
PspGI CCWGG 1 cut(s) 761
PspPI GGNCC 2 cut(s) 470, 781
PvuI CGATCG 1 cut(s) 166
PvuII CAGCTG 1 cut(s) 660
RsaI GTAC 1 cut(s) 683
RsaNI GTAC 1 cut(s) 682
SaqAI TTAA 3 cut(s) 201, 267, 770
SatI GCNGC 3 cut(s) 565, 658, 789
Sau3AI GATC 6 cut(s) 159, 163, 280, 749, 842, 861
Sau96I GGNCC 2 cut(s) 470, 781
SchI GAGTC 2 cut(s) 848, 923
ScrFI CCNGG 1 cut(s) 763
SduI GDGCHC 2 cut(s) 413, 921
SetI ASST 9 cut(s) 151, 309, 475, 502, 541, 662, 940, 969, 978
Sfr274I CTCGAG 1 cut(s) 308
SinI GGWCC 2 cut(s) 470, 781
SlaI CTCGAG 1 cut(s) 308
SmlI CTYRAG 1 cut(s) 308
SmoI CTYRAG 1 cut(s) 308
Sse9I AATT 2 cut(s) 224, 547
SsiI CCGC 5 cut(s) 339, 636, 678, 788, 924
SspMI CTAG 1 cut(s) 809
StyD4I CCNGG 1 cut(s) 761
StyI CCWWGG 1 cut(s) 325
TaaI ACNGT 4 cut(s) 241, 425, 596, 899
TaqI TCGA 5 cut(s) 108, 162, 217, 309, 841
TasI AATT 2 cut(s) 224, 547
TauI GCSGC 1 cut(s) 791
TfiI GAWTC 1 cut(s) 512
Tru1I TTAA 3 cut(s) 201, 267, 770
Tru9I TTAA 3 cut(s) 201, 267, 770
TscAI CASTG 2 cut(s) 123, 650
TseI GCWGC 2 cut(s) 564, 657
TspDTI ATGAA 5 cut(s) 17, 59, 633, 731, 766
TspRI CASTG 2 cut(s) 123, 650
VneI GTGCAC 1 cut(s) 917
VpaK11BI GGWCC 2 cut(s) 470, 781
XapI RAATTY 1 cut(s) 547
XceI RCATGY 1 cut(s) 889
XhoI CTCGAG 1 cut(s) 308
XspI CTAG 1 cut(s) 809
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.