Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr5A

Orthogroup ID

OG0007727

Physical Location & Seq

Genomic Coordinates Reverse (-)

16417352 .. 16422307

Gene Structure 4956 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh5AG156100.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1485 bp

ATGGATTTGTCAGCGAAGTTTACATTTTTACTCGTGGTATTGGCTGTTCTTGAACAACATGTTGTTTATGGACGGTTTGTGGTGGAGACGGGTAGTATTAAAGTTCTGTATCCAATGACTCTGAGGGGTAATCATGATGGTGCTATTGGAAACTTTGGTCTGCCGGACTATGGAGGCTCCTTAGTGGGTACTGTGGTGTATCCTGAGAAAGGGTCTACTGGGTGTGAAGCTTTTGAGGGTGATAAGCCTTTCAAGTCTCAGAAGTCTCGCCCCAGTATTGTTGTTCTTGATCGTGGTGGTTGCTACTTTGCTTTGAAGGTGTGGAATGCTCAAGAGGCAGGAGCTACAGCCGTTTTAGTGGCTGACAATGTTGAAGAGCCTCTAATTACAATGGACTCCCCAGAGGAGAGCAATGATGCAGATGGTTACATAGAGAAGATTGGAATTCCATCAGCTTTGATAGAGAAATCATTTGGTGACAGCTTGAAGGATGCTGTGAAAAACAGTGAAGATGTTGTGGTGAAACTCGACTGGAGAGAGTCAGTGCCCCATCCTGATAAAAGAGTTGAGTATGAGTTCTGGACAAACAGCAATGACGAATGCGGGGCTCGCTGTGATGAGCAAATGAGTTTTGTGAAAAATTTCAAGGGTCATGCTCAGATTCTTGAGAAAGGGGGTTACACCCTCTTCACTCCACACTATATAACTTGGTATTGCCCTAAGGCTTTTGTCCTTACCGCTCAATGCAAGTCTCAATGCATAAACCGGGGGAGATACTGTGCACCAGATCCGGAGAAAGATTTTGGAGAAGGATACCAAGGGAAGGATGTGGTATTTGAGAACTTGAGGCAGCTTTGTGTGCATAGAGTTGCCGAGGAGAGAAACCGGTCATGGGTCTGGTGGGATTATGTGACAGACTTCCATATCAGATGTTCCATGAAAGAAAAGCGTTACAGTAAAGAATGCGCTGAGGATGTCATGAAATCACTTGATTTGCCTATTGACAAGATTAAAAAATGCATGGGCAATCCTGAAGATGATGTGGAGAATGAAGTGCTTAAAGCTGAACAAGAAATCCAGGTTGGTCGAGGATCTCGTGGTGATGTTACCATCTTGCCAACATTGGTGATTAACAATGTTCAATATCGAGGAAAACTGGAACGAACTGGTGTCCTAAAGGCACTGTGTGCTGGATTCAAGGAGACAACTGAACCTCAAATTTGTTTGAACGGAGATCTTGAAACAAATGAGTGTCTAGAGAGGAATGGTGGCTGTTGGCTGGACAACCGGAGTAATTTAACTGCTTGCAAGGACACTTTTAGGGGAAGAGTTTGTGAGTGCCCTGTAGTGGATGGTGTTCAATATAAAGGCGATGGTTATATGTCTTGTGCTGCTTTTGGACCAGCAAGGTGCACGATTGACAATGGAGGTTGCTGGTCAGAATATAGAAATGGATTAACTATATCTGCTTGCTCAGTTCAGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PA cEGF

Protein Analysis

494

Amino Acids

55.16

Weight (kDa)

5.16

Isoelectric Point (pI)

34.52

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PA	PF02225	63 - 162	3.5e-17	PA domain
VSR_TRX	PF25011	189 - 396	1.6e-102	Vacuolar sorting receptor thioredoxin-like domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0007727)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G30900 AT2G34940 AT4G20110 AT4G20110
fragaria_vesca	FvH4_3g13190 FvH4_3g13190
malus_domestica	MD05G1244500.v1.1 MD10G1226100.v1.1
prunus_persica	Prupe.4G119200_v2.0.a1 Prupe.4G119200_v2.0.a1 Prupe.4G119200_v2.0.a1
pyrus_communis	pycom05g22100 pycom10g19000
rosa_chinensis	RchiOBHm_Chr5g0021401
rosa_laevigata	RLG00000032610
rosa_multiflora	Rmu_co8489967.1_g000001 Rmu_sc0004847.1_g000021
rosa_roxburghii	Rroxscaffold_1G00057140
rosa_rugosa	Rorug05G0067000
rosa_samantha	Rh5AG156100 Rh5BG155600 Rh5CG169300 Rh5DG156100
rosa_wichuraiana	Rw5G013900

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccBSI	CCGCTC	1 cut(s)	740
AccI	GTMKAC	1 cut(s)	215
AccIII	TCCGGA	1 cut(s)	790
AciI	CCGC	2 cut(s)	603, 738
AclWI	GGATC	2 cut(s)	782, 1099
AcsI	RAATTY	3 cut(s)	444, 640, 1218
AcuI	CTGAAG	1 cut(s)	1053
AdeI	CACNNNGTG	1 cut(s)	1187
AfaI	GTAC	1 cut(s)	190
AfiI	CCNNNNNNNGG	5 cut(s)	170, 209, 823, 892, 1348
AflIII	ACRYGT	1 cut(s)	58
AgeI	ACCGGT	1 cut(s)	885
AjnI	CCWGG	1 cut(s)	1077
AjuI	GAANNNNNNNTTGG	2 cut(s)	1065, 1097
AloI	GAACNNNNNNTCC	2 cut(s)	1059, 1091
AluBI	AGCT	6 cut(s)	230, 344, 455, 483, 853, 1064
AluI	AGCT	6 cut(s)	230, 344, 455, 483, 853, 1064
Alw21I	GWGCWC	2 cut(s)	784, 1415
Alw26I	GTCTC	5 cut(s)	80, 261, 270, 756, 1196
Alw44I	GTGCAC	2 cut(s)	780, 1411
AlwI	GGATC	2 cut(s)	782, 1099
Aor13HI	TCCGGA	1 cut(s)	790
ApaLI	GTGCAC	2 cut(s)	780, 1411
ApeKI	GCWGC	2 cut(s)	850, 1391
ApoI	RAATTY	3 cut(s)	444, 640, 1218
AsiGI	ACCGGT	1 cut(s)	885
Asp700I	GAANNNNTTC	1 cut(s)	641
AspLEI	GCGC	1 cut(s)	968
AspS9I	GGNCC	1 cut(s)	1400
AsuC2I	CCSGG	1 cut(s)	767
AsuHPI	GGTGA	5 cut(s)	251, 488, 532, 1112, 1138
AvaII	GGWCC	1 cut(s)	1400
AxyI	CCTNAGG	1 cut(s)	720
BaeGI	GKGCMC	4 cut(s)	549, 784, 1343, 1415
BaeI	ACNNNNGTAYC	2 cut(s)	180, 213
BanII	GRGCYC	1 cut(s)	610
BauI	CACGAG	2 cut(s)	32, 1095
Bbv12I	GWGCWC	2 cut(s)	784, 1415
BbvCI	CCTCAGC	1 cut(s)	969
BbvI	GCAGC	2 cut(s)	862, 1378
BccI	CCATC	7 cut(s)	131, 416, 457, 558, 1118, 1346, 1367
BceAI	ACGGC	1 cut(s)	335
BciT130I	CCWGG	1 cut(s)	1079
BciVI	GTATCC	3 cut(s)	120, 210, 806
BcnI	CCSGG	1 cut(s)	767
BcoDI	GTCTC	5 cut(s)	80, 261, 270, 756, 1196
BfaI	CTAG	1 cut(s)	1256
BfmI	CTRYAG	2 cut(s)	345, 1344
BfuI	GTATCC	3 cut(s)	120, 210, 806
BglII	AGATCT	1 cut(s)	1234
BisI	GCNGC	2 cut(s)	851, 1392
BlsI	GCNGC	2 cut(s)	852, 1393
Bme1390I	CCNGG	2 cut(s)	767, 1079
Bme18I	GGWCC	1 cut(s)	1400
BmgT120I	GGNCC	1 cut(s)	1400
BmiI	GGNNCC	1 cut(s)	178
BmrFI	CCNGG	2 cut(s)	767, 1079
BmrI	ACTGGG	2 cut(s)	228, 267
BmsI	GCATC	2 cut(s)	406, 481
BmuI	ACTGGG	2 cut(s)	228, 267
BpmI	CTGGAG	1 cut(s)	553
Bpu10I	CCTNAGC	1 cut(s)	969
BpuEI	CTTGAG	3 cut(s)	315, 686, 865
BpuMI	CCSGG	1 cut(s)	767
BsaJI	CCNNGG	3 cut(s)	766, 817, 873
BsaWI	WCCGGW	3 cut(s)	790, 885, 1287
BsaXI	ACNNNNNCTCC	2 cut(s)	763, 793
Bsc4I	CCNNNNNNNGG	5 cut(s)	170, 209, 823, 892, 1348
Bse118I	RCCGGY	1 cut(s)	885
Bse1I	ACTGG	5 cut(s)	223, 273, 536, 1161, 1171
Bse21I	CCTNAGG	1 cut(s)	720
Bse3DI	GCAATG	2 cut(s)	418, 598
BseAI	TCCGGA	1 cut(s)	790
BseBI	CCWGG	1 cut(s)	1079
BseDI	CCNNGG	3 cut(s)	766, 817, 873
BseGI	GGATG	5 cut(s)	496, 550, 832, 979, 1357
BseLI	CCNNNNNNNGG	5 cut(s)	170, 209, 823, 892, 1348
BseMI	GCAATG	2 cut(s)	418, 598
BseMII	CTCAG	5 cut(s)	113, 195, 272, 671, 960
BseNI	ACTGG	5 cut(s)	223, 273, 536, 1161, 1171
BseRI	GAGGAG	2 cut(s)	419, 890
BseSI	GKGCMC	4 cut(s)	549, 784, 1343, 1415
BseXI	GCAGC	2 cut(s)	862, 1378
BshTI	ACCGGT	1 cut(s)	885
BsiHKAI	GWGCWC	2 cut(s)	784, 1415
BsiSI	CCGG	5 cut(s)	164, 766, 791, 886, 1288
BslI	CCNNNNNNNGG	5 cut(s)	170, 209, 823, 892, 1348
BsmAI	GTCTC	5 cut(s)	80, 261, 270, 756, 1196
BsmBI	CGTCTC	1 cut(s)	80
BsmI	GAATGC	3 cut(s)	331, 605, 968
Bsp1286I	GDGCHC	5 cut(s)	549, 610, 784, 1343, 1415
Bsp13I	TCCGGA	1 cut(s)	790
Bsp143I	GATC	4 cut(s)	289, 787, 1091, 1234
BspACI	CCGC	2 cut(s)	603, 738
BspCNI	CTCAG	5 cut(s)	114, 196, 271, 670, 961
BspEI	TCCGGA	1 cut(s)	790
BspHI	TCATGA	2 cut(s)	133, 978
BspLI	GGNNCC	1 cut(s)	178
BspPI	GGATC	2 cut(s)	782, 1099
BspQI	GCTCTTC	1 cut(s)	369
BsrBI	CCGCTC	1 cut(s)	740
BsrDI	GCAATG	2 cut(s)	418, 598
BsrFI	RCCGGY	1 cut(s)	885
BsrI	ACTGG	5 cut(s)	223, 273, 536, 1161, 1171
BssAI	RCCGGY	1 cut(s)	885
BssECI	CCNNGG	3 cut(s)	766, 817, 873
BssMI	GATC	4 cut(s)	289, 787, 1091, 1234
BssSI	CACGAG	2 cut(s)	32, 1095
BssT1I	CCWWGG	1 cut(s)	817
Bst2BI	CACGAG	2 cut(s)	32, 1095
Bst2UI	CCWGG	1 cut(s)	1079
Bst4CI	ACNGT	6 cut(s)	75, 193, 506, 779, 956, 1185
Bst6I	CTCTTC	3 cut(s)	369, 692, 1321
BstAPI	GCANNNNNTGC	1 cut(s)	1187
BstC8I	GCNNGC	3 cut(s)	610, 1306, 1471
BstDEI	CTNAG	8 cut(s)	122, 181, 204, 258, 657, 720, 969, 1474
BstENI	CCTNNNNNAGG	1 cut(s)	207
BstF5I	GGATG	5 cut(s)	496, 550, 832, 979, 1357
BstHHI	GCGC	1 cut(s)	968
BstKTI	GATC	4 cut(s)	292, 790, 1094, 1237
BstMAI	GTCTC	5 cut(s)	80, 261, 270, 756, 1196
BstMBI	GATC	4 cut(s)	289, 787, 1091, 1234
BstMWI	GCNNNNNNNGC	4 cut(s)	335, 609, 859, 1187
BstNI	CCWGG	1 cut(s)	1079
BstNSI	RCATGY	1 cut(s)	62
BstSCI	CCNGG	2 cut(s)	765, 1077
BstSFI	CTRYAG	2 cut(s)	345, 1344
BstSLI	GKGCMC	4 cut(s)	549, 784, 1343, 1415
BstV1I	GCAGC	2 cut(s)	862, 1378
BstX2I	RGATCY	3 cut(s)	787, 1091, 1234
BstYI	RGATCY	3 cut(s)	787, 1091, 1234
Bsu36I	CCTNAGG	1 cut(s)	720
BsuI	GTATCC	3 cut(s)	120, 210, 806
BtgZI	GCGATG	1 cut(s)	1386
BtsCI	GGATG	5 cut(s)	496, 550, 832, 979, 1357
BtsIMutI	CAGTG	3 cut(s)	511, 549, 1181
Cac8I	GCNNGC	3 cut(s)	610, 1306, 1471
CciI	TCATGA	2 cut(s)	133, 978
CfoI	GCGC	1 cut(s)	968
Cfr10I	RCCGGY	1 cut(s)	885
Cfr13I	GGNCC	1 cut(s)	1400
Csp6I	GTAC	1 cut(s)	189
CspAI	ACCGGT	1 cut(s)	885
CviAII	CATG	7 cut(s)	59, 134, 653, 891, 937, 979, 1021
CviQI	GTAC	1 cut(s)	189
DdeI	CTNAG	8 cut(s)	122, 181, 204, 258, 657, 720, 969, 1474
DpnI	GATC	4 cut(s)	291, 789, 1093, 1236
DpnII	GATC	4 cut(s)	289, 787, 1091, 1234
DraIII	CACNNNGTG	1 cut(s)	1187
Eam1104I	CTCTTC	3 cut(s)	369, 692, 1321
EarI	CTCTTC	3 cut(s)	369, 692, 1321
Eco130I	CCWWGG	1 cut(s)	817
Eco24I	GRGCYC	1 cut(s)	610
Eco47I	GGWCC	1 cut(s)	1400
Eco57I	CTGAAG	1 cut(s)	1053
Eco81I	CCTNAGG	1 cut(s)	720
EcoNI	CCTNNNNNAGG	1 cut(s)	207
EcoRI	GAATTC	1 cut(s)	444
EcoRII	CCWGG	1 cut(s)	1077
EcoT14I	CCWWGG	1 cut(s)	817
EcoT22I	ATGCAT	2 cut(s)	761, 1022
EcoT38I	GRGCYC	1 cut(s)	610
ErhI	CCWWGG	1 cut(s)	817
Esp3I	CGTCTC	1 cut(s)	80
FaeI	CATG	7 cut(s)	62, 137, 656, 894, 940, 982, 1024
FatI	CATG	7 cut(s)	58, 133, 652, 890, 936, 978, 1020
FauI	CCCGC	1 cut(s)	596
FblI	GTMKAC	1 cut(s)	215
Fnu4HI	GCNGC	2 cut(s)	851, 1392
FokI	GGATG	5 cut(s)	503, 537, 839, 986, 1364
FriOI	GRGCYC	1 cut(s)	610
Fsp4HI	GCNGC	2 cut(s)	851, 1392
FspBI	CTAG	1 cut(s)	1256
GlaI	GCGC	1 cut(s)	967
GluI	GCNGC	2 cut(s)	851, 1392
GsuI	CTGGAG	1 cut(s)	553
HapII	CCGG	5 cut(s)	164, 766, 791, 886, 1288
HhaI	GCGC	1 cut(s)	968
Hin1II	CATG	7 cut(s)	62, 137, 656, 894, 940, 982, 1024
Hin6I	GCGC	1 cut(s)	966
HinP1I	GCGC	1 cut(s)	966
HindIII	AAGCTT	1 cut(s)	228
HinfI	GANTC	5 cut(s)	118, 395, 539, 661, 1194
HpaII	CCGG	5 cut(s)	164, 766, 791, 886, 1288
HphI	GGTGA	5 cut(s)	251, 488, 532, 1112, 1138
Hpy166II	GTNNAC	4 cut(s)	21, 216, 782, 1413
Hpy188I	TCNGA	5 cut(s)	123, 261, 660, 929, 1441
Hpy8I	GTNNAC	4 cut(s)	21, 216, 782, 1413
HpyAV	CCTTC	4 cut(s)	310, 481, 803, 817
HpyCH4III	ACNGT	6 cut(s)	75, 193, 506, 779, 956, 1185
HpyCH4V	TGCA	8 cut(s)	419, 747, 759, 782, 862, 1020, 1308, 1413
HpyF10VI	GCNNNNNNNGC	4 cut(s)	335, 609, 859, 1187
HpyF3I	CTNAG	8 cut(s)	122, 181, 204, 258, 657, 720, 969, 1474
Hsp92II	CATG	7 cut(s)	62, 137, 656, 894, 940, 982, 1024
HspAI	GCGC	1 cut(s)	966
Kpn2I	TCCGGA	1 cut(s)	790
Kzo9I	GATC	4 cut(s)	289, 787, 1091, 1234
LguI	GCTCTTC	1 cut(s)	369
LmnI	GCTCC	2 cut(s)	182, 341
Lsp1109I	GCAGC	2 cut(s)	862, 1378
LweI	GCATC	2 cut(s)	406, 481
MaeI	CTAG	1 cut(s)	1256
MaeIII	GTNAC	6 cut(s)	425, 476, 677, 910, 950, 1105
MalI	GATC	4 cut(s)	291, 789, 1093, 1236
MbiI	CCGCTC	1 cut(s)	740
MboI	GATC	4 cut(s)	289, 787, 1091, 1234
MboII	GAAGA	6 cut(s)	386, 448, 521, 679, 1046, 1338
MflI	RGATCY	3 cut(s)	787, 1091, 1234
MhlI	GDGCHC	5 cut(s)	549, 610, 784, 1343, 1415
MluCI	AATT	5 cut(s)	384, 444, 640, 1218, 1294
MlyI	GAGTC	3 cut(s)	112, 389, 548
Mph1103I	ATGCAT	2 cut(s)	761, 1022
MroI	TCCGGA	1 cut(s)	790
MroXI	GAANNNNTTC	1 cut(s)	641
MseI	TTAA	6 cut(s)	99, 1011, 1059, 1131, 1298, 1457
MslI	CAYNNNNRTG	1 cut(s)	138
MspI	CCGG	5 cut(s)	164, 766, 791, 886, 1288
MspR9I	CCNGG	2 cut(s)	767, 1079
Mva1269I	GAATGC	3 cut(s)	331, 605, 968
MvaI	CCWGG	1 cut(s)	1079
MwoI	GCNNNNNNNGC	4 cut(s)	335, 609, 859, 1187
NciI	CCSGG	1 cut(s)	767
NdeII	GATC	4 cut(s)	289, 787, 1091, 1234
NlaIII	CATG	7 cut(s)	62, 137, 656, 894, 940, 982, 1024
NlaIV	GGNNCC	1 cut(s)	178
NmeAIII	GCCGAG	1 cut(s)	898
NmuCI	GTSAC	2 cut(s)	476, 910
NsiI	ATGCAT	2 cut(s)	761, 1022
NspI	RCATGY	1 cut(s)	62
PagI	TCATGA	2 cut(s)	133, 978
PciI	ACATGT	1 cut(s)	58
PciSI	GCTCTTC	1 cut(s)	369
PcsI	WCGNNNNNNNCGW	1 cut(s)	1093
PctI	GAATGC	3 cut(s)	331, 605, 968
PdmI	GAANNNNTTC	1 cut(s)	641
PfeI	GAWTC	2 cut(s)	661, 1194
PinAI	ACCGGT	1 cut(s)	885
PkrI	GCNGC	2 cut(s)	852, 1393
PleI	GAGTC	3 cut(s)	112, 389, 547
PpsI	GAGTC	3 cut(s)	112, 389, 547
PscI	ACATGT	1 cut(s)	58
Psp6I	CCWGG	1 cut(s)	1077
PspGI	CCWGG	1 cut(s)	1077
PspN4I	GGNNCC	1 cut(s)	178
PspPI	GGNCC	1 cut(s)	1400
PsrI	GAACNNNNNNTAC	2 cut(s)	30, 62
PsuI	RGATCY	3 cut(s)	787, 1091, 1234
RsaI	GTAC	1 cut(s)	190
RsaNI	GTAC	1 cut(s)	189
RseI	CAYNNNNRTG	1 cut(s)	138
SapI	GCTCTTC	1 cut(s)	369
SaqAI	TTAA	6 cut(s)	99, 1011, 1059, 1131, 1298, 1457
SatI	GCNGC	2 cut(s)	851, 1392
Sau3AI	GATC	4 cut(s)	289, 787, 1091, 1234
Sau96I	GGNCC	1 cut(s)	1400
SchI	GAGTC	3 cut(s)	112, 389, 548
ScrFI	CCNGG	2 cut(s)	767, 1079
SduI	GDGCHC	5 cut(s)	549, 610, 784, 1343, 1415
SfaNI	GCATC	2 cut(s)	406, 481
SfcI	CTRYAG	2 cut(s)	345, 1344
SinI	GGWCC	1 cut(s)	1400
SmiMI	CAYNNNNRTG	1 cut(s)	138
SmlI	CTYRAG	3 cut(s)	330, 665, 844
SmoI	CTYRAG	3 cut(s)	330, 665, 844
Sse9I	AATT	5 cut(s)	384, 444, 640, 1218, 1294
SsiI	CCGC	2 cut(s)	603, 738
SspMI	CTAG	1 cut(s)	1256
StyD4I	CCNGG	2 cut(s)	765, 1077
StyI	CCWWGG	1 cut(s)	817
TaaI	ACNGT	6 cut(s)	75, 193, 506, 779, 956, 1185
TaqI	TCGA	3 cut(s)	528, 1087, 1147
TasI	AATT	5 cut(s)	384, 444, 640, 1218, 1294
TfiI	GAWTC	2 cut(s)	661, 1194
Tru1I	TTAA	6 cut(s)	99, 1011, 1059, 1131, 1298, 1457
Tru9I	TTAA	6 cut(s)	99, 1011, 1059, 1131, 1298, 1457
TscAI	CASTG	3 cut(s)	511, 549, 1188
TseFI	GTSAC	2 cut(s)	476, 910
TseI	GCWGC	2 cut(s)	850, 1391
Tsp45I	GTSAC	2 cut(s)	476, 910
TspDTI	ATGAA	3 cut(s)	953, 995, 1065
TspGWI	ACGGA	1 cut(s)	1245
TspRI	CASTG	3 cut(s)	511, 549, 1188
VneI	GTGCAC	2 cut(s)	780, 1411
VpaK11BI	GGWCC	1 cut(s)	1400
XagI	CCTNNNNNAGG	1 cut(s)	207
XapI	RAATTY	3 cut(s)	444, 640, 1218
XbaI	TCTAGA	1 cut(s)	1255
XceI	RCATGY	1 cut(s)	62
XmiI	GTMKAC	1 cut(s)	215
XmnI	GAANNNNTTC	1 cut(s)	641
XspI	CTAG	1 cut(s)	1256
Zsp2I	ATGCAT	2 cut(s)	761, 1022

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.