Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr10

Orthogroup ID

OG0007727

Physical Location & Seq

Genomic Coordinates Forward (+)

22208216 .. 22212706

Gene Structure 4491 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom10g19000.9

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2316 bp

ATGTCTTCGCTTTCCCGGTCTTTTCTCTACCATGCCAAGATTCCTAGCAGTAGAAGTGGTTCCGTAGAAGTGGTTCCAATGACGCAGTCAACCACCCTCCTTCCCTCTCACCAGTCGTTTCCTTTACGTTACAGATTGAAAGAGAAAGTTGAACCCTCCTCTTACACGACAAACAATCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTATGTGTGAACCAAACCAACCAAAACCTTTCTTATTATCTAGTCTTCCACTCTCCCTCTACCCGTAGACCGTTGCGAGGTTCATTCAAAGATACGCCCCCATTTCTTCCACCTCCACCTTCTCTAGCTCTCAAACCCCCAAAACACAGAAAACCCGCTTCTCATTTTCCCAAAAAAAACAGAGGAAAGAGAAAAATCTCTGCTTGCATGGCTTTTCTCCCAAAGCTCGCAGCTTTTTTCGTGGTGTTATTATCATTTTTTGAGAACCATGTGCATGCAAGGTTTGTGGTGGAGACGGGAAGCGTTAGGGTTCTGTATCCAATGAGCCTGCGCTCGAAGCACGACGGAGCTATCGGCAACTTTGGGCTGCCGGACTATGGCGGCTCCTTGGTGGGCACTGTTGTCTATCCTGAAAAAGGGTCTTCCGGCTGTGAAGCTTTTGAGGGTGACAAGCCTTTCAAGTCGAAAAATTCTCGCTCCACCATTGTTCTTCTTGATCGCGGAGGTTGCTACTTTGCTTTGAAGGTGTGGAATGCTCAACAGGCTGGAGCCTCAGCCGTTTTAGTGGCAGATAACATTGAAGAGCCTCTAATAACAATGGATTCCCCGGAAGAGAGCACTGATGCAGATGGTTACATAGAGAAGATTGGAATTCCGTCAGCTTTGATAGAGAAAGCTTTTGGTGACAGCTTGAAGGAAGCACTAAAGAACAAGGAAGATGTTGTGATGAAACTAGACTGGAGAGAGTCAGTGCCACACCCCGATCGAAGAGTTGAATACGAGTTCTGGACAAACAGCAACGATGAGTGTGGGGCTCGTTGTGATGAGCAAATGGCTTTTGTGAAAAATTTCAAGGGTCATGCTCAAATTCTGGAGAGAGGGGGTTACACGCTCTTCACTCCACATTATATAACTTGGTATTGTCCTCGGGCATTTGTCCTCACAACTCAGTGCAAGTCCCAATGCATAAACCATGGGAGATATTGTGCACCAGATCCTGAGAGAGATTTAGGAGAGGGGTACCAAGGGAAGGATGTGGTATATGAGAACTTGAGGCAGCTTTGTGTGCATAGAGTTGCCAATGAGAGTGGCCGGTCATGGGTTTGGTGGGATTATGTGACAGACTTCCATATCAGATGTTCCATGAAAGAAAAGAAGTATAGCAAAGATTGCGCCGAGGATGTCATGAAATCACTTGATTTGCCTGTTGACAAGATAAAAAAATGTATGGGTAATCCTGAAGATGATGTCGAGAATGCAGTACTCAAAGCCGAACAAGAAATCCAGGTTGGAAGAGGATCTCGTGGTGACATTACCATCTTGCCGACATTGGTTATTAACAATGCTCAGTTTCGAGGAAAATTAGATAGAACTGCTGTGCTAAAGGCCATATGTGCTGGATTTAAGGAGACGACTGATCCTCCTATTTGTTTGAGTGGAGAACTTGAGACAAATGAGTGCCTTGAGAGGAATGGTGGCTGTTGGCAGGACAGCCAATATAATATAACTGCTTGCAAGGACACATTCAGGGGAAGAGTTTGCGAGTGCCCTCTAGTGAACGGTGTTCAGTATAGAGGCGATGGTTATACATCTTGTGATGCTTTCGGACCTGCAAGATGTGCAATAAACAATGGAGGTTGCTGGTCAGAATCTAGAAATGGATTGCATTTATCGGCATGCTCAAACTCTGAGTTATCCGGCTGCAAATGTCCACAAGGCTTTCATGGGGACGGTCATAAATGCGAAGATATTGATGAATGCAAGGCGCGTAGTGCTTGTCAGTGTGATGGCTGCAGCTGTAAGAACACTTGGGGCGGGTACAACTGCAAATGTAAGGGAGGCCAGCTGTATATGAAGGAGCAGGATACTTGTATTGAGACATGGATCGAGATTTGCCTGGTTTTTAACTTTCATGGTCCTTGCGGCTGTGTCAACTGTTGGCCTAGCTGGTTACATCTTCTACAAATACAGGCTTCGATCTTACATGGACTCCGAGATCATGGCTATCATGTCGCAATACATGCCTCTGGACAGCCAGCAGGCCAGTCAAGTACATACCGCCGACTCAGAACCTCTACGACGAGGCTCAGTATAAGGAACACAAGCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PA cEGF

Protein Analysis

772

Amino Acids

85.97

Weight (kDa)

8.24

Isoelectric Point (pI)

44.33

Instability Index

Protein Domains (Pfam)

No Pfam domains detected for this protein.

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0007727)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G30900 AT2G34940 AT4G20110 AT4G20110
fragaria_vesca	FvH4_3g13190 FvH4_3g13190
malus_domestica	MD05G1244500.v1.1 MD10G1226100.v1.1
prunus_persica	Prupe.4G119200_v2.0.a1 Prupe.4G119200_v2.0.a1 Prupe.4G119200_v2.0.a1
pyrus_communis	pycom05g22100 pycom10g19000
rosa_chinensis	RchiOBHm_Chr5g0021401
rosa_laevigata	RLG00000032610
rosa_multiflora	Rmu_co8489967.1_g000001 Rmu_sc0004847.1_g000021
rosa_roxburghii	Rroxscaffold_1G00057140
rosa_rugosa	Rorug05G0067000
rosa_samantha	Rh5AG156100 Rh5BG155600 Rh5CG169300 Rh5DG156100
rosa_wichuraiana	Rw5G013900

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	1825
Acc65I	GGTACC	1 cut(s)	1227
AccB1I	GGYRCC	1 cut(s)	1227
AccI	GTMKAC	1 cut(s)	274
AccII	CGCG	2 cut(s)	708, 1975
AciI	CCGC	6 cut(s)	363, 588, 708, 2022, 2130, 2266
AclWI	GGATC	4 cut(s)	1196, 1513, 1619, 2099
AcoI	YGGCCR	1 cut(s)	1297
AcsI	RAATTY	4 cut(s)	676, 858, 1054, 1074
AcuI	CTGAAG	1 cut(s)	1467
AfaI	GTAC	4 cut(s)	1229, 1470, 2027, 2260
AfiI	CCNNNNNNNGG	5 cut(s)	284, 584, 623, 1237, 1306
AgsI	TTSAA	9 cut(s)	139, 152, 295, 667, 730, 788, 901, 983, 1060
AjnI	CCWGG	2 cut(s)	1491, 2103
AjuI	GAANNNNNNNTTGG	2 cut(s)	1479, 1511
AloI	GAACNNNNNNTCC	2 cut(s)	1473, 1505
Alw21I	GWGCWC	2 cut(s)	827, 1198
Alw26I	GTCTC	4 cut(s)	494, 1610, 1649, 2078
Alw44I	GTGCAC	1 cut(s)	1194
AlwI	GGATC	4 cut(s)	1196, 1513, 1619, 2099
AlwNI	CAGNNNCTG	1 cut(s)	1205
Ama87I	CYCGRG	1 cut(s)	1134
AoxI	GGCC	5 cut(s)	1297, 1593, 2047, 2147, 2248
ApaLI	GTGCAC	1 cut(s)	1194
ApeKI	GCWGC	6 cut(s)	437, 574, 1264, 1908, 1998, 2001
ApoI	RAATTY	4 cut(s)	676, 858, 1054, 1074
Asp700I	GAANNNNTTC	3 cut(s)	58, 72, 1055
Asp718I	GGTACC	1 cut(s)	1227
AspLEI	GCGC	3 cut(s)	540, 1382, 1975
AspS9I	GGNCC	2 cut(s)	1814, 2123
AsuC2I	CCSGG	2 cut(s)	16, 815
AsuHPI	GGTGA	4 cut(s)	101, 665, 902, 1526
AvaI	CYCGRG	1 cut(s)	1134
AvaII	GGWCC	2 cut(s)	1814, 2123
BaeGI	GKGCMC	3 cut(s)	605, 1198, 1757
BanI	GGYRCC	1 cut(s)	1227
BanII	GRGCYC	1 cut(s)	1024
BauI	CACGAG	1 cut(s)	1509
BbsI	GAAGAC	2 cut(s)	244, 621
Bbv12I	GWGCWC	2 cut(s)	827, 1198
BbvCI	CCTCAGC	1 cut(s)	760
BbvI	GCAGC	6 cut(s)	449, 561, 1276, 1895, 1985, 2013
BccI	CCATC	4 cut(s)	830, 1532, 1781, 1988
BceAI	ACGGC	1 cut(s)	749
BciT130I	CCWGG	2 cut(s)	1493, 2105
BciVI	GTATCC	2 cut(s)	534, 2065
BcnI	CCSGG	2 cut(s)	16, 815
BcoDI	GTCTC	4 cut(s)	494, 1610, 1649, 2078
BfaI	CTAG	7 cut(s)	45, 248, 332, 941, 1760, 1860, 2151
BfmI	CTRYAG	1 cut(s)	1999
BfuAI	ACCTGC	1 cut(s)	1825
BfuI	GTATCC	2 cut(s)	534, 2065
BisI	GCNGC	8 cut(s)	438, 575, 589, 1265, 1909, 1999, 2002, 2131
BlsI	GCNGC	8 cut(s)	439, 576, 590, 1266, 1910, 2000, 2003, 2132
BmcAI	AGTACT	1 cut(s)	1470
Bme1390I	CCNGG	4 cut(s)	16, 815, 1493, 2105
Bme18I	GGWCC	2 cut(s)	1814, 2123
BmeT110I	CYCGRG	1 cut(s)	1134
BmgT120I	GGNCC	2 cut(s)	1814, 2123
BmiI	GGNNCC	5 cut(s)	61, 75, 592, 757, 1229
BmrFI	CCNGG	4 cut(s)	16, 815, 1493, 2105
BmsI	GCATC	2 cut(s)	820, 1795
BpiI	GAAGAC	2 cut(s)	244, 621
BpmI	CTGGAG	3 cut(s)	774, 967, 1100
Bpu10I	CCTNAGC	1 cut(s)	760
BpuEI	CTTGAG	3 cut(s)	1279, 1673, 1691
BpuMI	CCSGG	2 cut(s)	16, 815
BsaJI	CCNNGG	6 cut(s)	594, 813, 1133, 1180, 1231, 1383
BsaXI	ACNNNNNCTCC	6 cut(s)	1177, 1207, 1612, 1642, 2181, 2211
Bsc4I	CCNNNNNNNGG	5 cut(s)	284, 584, 623, 1237, 1306
Bse118I	RCCGGY	1 cut(s)	1299
Bse1I	ACTGG	3 cut(s)	112, 950, 2251
BseBI	CCWGG	2 cut(s)	1493, 2105
BseDI	CCNNGG	6 cut(s)	594, 813, 1133, 1180, 1231, 1383
BseGI	GGATG	2 cut(s)	1246, 1393
BseLI	CCNNNNNNNGG	5 cut(s)	284, 584, 623, 1237, 1306
BseMII	CTCAG	7 cut(s)	774, 1169, 1197, 1568, 1887, 2287, 2308
BseNI	ACTGG	3 cut(s)	112, 950, 2251
BseRI	GAGGAG	1 cut(s)	148
BseSI	GKGCMC	3 cut(s)	605, 1198, 1757
BseXI	GCAGC	6 cut(s)	449, 561, 1276, 1895, 1985, 2013
Bsh1236I	CGCG	2 cut(s)	708, 1975
Bsh1285I	CGRYCG	1 cut(s)	973
BshFI	GGCC	5 cut(s)	1299, 1595, 2049, 2149, 2250
BshNI	GGYRCC	1 cut(s)	1227
BsiEI	CGRYCG	1 cut(s)	973
BsiHKAI	GWGCWC	2 cut(s)	827, 1198
BsiHKCI	CYCGRG	1 cut(s)	1134
BsiSI	CCGG	6 cut(s)	16, 578, 633, 815, 1300, 1905
BslFI	GGGAC	2 cut(s)	1150, 1949
BslI	CCNNNNNNNGG	5 cut(s)	284, 584, 623, 1237, 1306
BsmAI	GTCTC	4 cut(s)	494, 1610, 1649, 2078
BsmBI	CGTCTC	2 cut(s)	494, 1610
BsmFI	GGGAC	2 cut(s)	1150, 1949
BsmI	GAATGC	3 cut(s)	745, 1468, 1970
BsnI	GGCC	5 cut(s)	1299, 1595, 2049, 2149, 2250
BsoBI	CYCGRG	1 cut(s)	1134
Bsp1286I	GDGCHC	5 cut(s)	605, 827, 1024, 1198, 1757
Bsp143I	GATC	8 cut(s)	703, 970, 1201, 1505, 1624, 2091, 2184, 2203
Bsp19I	CCATGG	1 cut(s)	1180
BspACI	CCGC	6 cut(s)	363, 588, 708, 2022, 2130, 2266
BspANI	GGCC	5 cut(s)	1299, 1595, 2049, 2149, 2250
BspCNI	CTCAG	7 cut(s)	773, 1168, 1198, 1567, 1888, 2286, 2307
BspFNI	CGCG	2 cut(s)	708, 1975
BspHI	TCATGA	1 cut(s)	1392
BspLI	GGNNCC	5 cut(s)	61, 75, 592, 757, 1229
BspMAI	CTGCAG	1 cut(s)	2003
BspMI	ACCTGC	1 cut(s)	1825
BspPI	GGATC	4 cut(s)	1196, 1513, 1619, 2099
BspQI	GCTCTTC	2 cut(s)	783, 1106
BspT107I	GGYRCC	1 cut(s)	1227
BsrFI	RCCGGY	1 cut(s)	1299
BsrI	ACTGG	3 cut(s)	112, 950, 2251
BssAI	RCCGGY	1 cut(s)	1299
BssECI	CCNNGG	6 cut(s)	594, 813, 1133, 1180, 1231, 1383
BssMI	GATC	8 cut(s)	703, 970, 1201, 1505, 1624, 2091, 2184, 2203
BssSI	CACGAG	1 cut(s)	1509
BssT1I	CCWWGG	3 cut(s)	594, 1180, 1231
Bst2BI	CACGAG	1 cut(s)	1509
Bst2UI	CCWGG	2 cut(s)	1493, 2105
Bst4CI	ACNGT	5 cut(s)	279, 607, 1769, 1940, 2144
Bst6I	CTCTTC	6 cut(s)	783, 813, 970, 1106, 1495, 1735
BstAPI	GCANNNNNTGC	3 cut(s)	1377, 1826, 2228
BstC8I	GCNNGC	9 cut(s)	412, 435, 483, 536, 1720, 1885, 2051, 2244, 2248
BstDEI	CTNAG	7 cut(s)	760, 1155, 1206, 1554, 1896, 2273, 2294
BstDSI	CCRYGG	1 cut(s)	1180
BstENI	CCTNNNNNAGG	1 cut(s)	621
BstF5I	GGATG	2 cut(s)	1246, 1393
BstFNI	CGCG	2 cut(s)	708, 1975
BstHHI	GCGC	3 cut(s)	540, 1382, 1975
BstKTI	GATC	8 cut(s)	706, 973, 1204, 1508, 1627, 2094, 2187, 2206
BstMAI	GTCTC	4 cut(s)	494, 1610, 1649, 2078
BstMBI	GATC	8 cut(s)	703, 970, 1201, 1505, 1624, 2091, 2184, 2203
BstMCI	CGRYCG	1 cut(s)	973
BstNI	CCWGG	2 cut(s)	1493, 2105
BstNSI	RCATGY	3 cut(s)	485, 1887, 2231
BstSCI	CCNGG	4 cut(s)	14, 813, 1491, 2103
BstSFI	CTRYAG	1 cut(s)	1999
BstSLI	GKGCMC	3 cut(s)	605, 1198, 1757
BstUI	CGCG	2 cut(s)	708, 1975
BstV1I	GCAGC	6 cut(s)	449, 561, 1276, 1895, 1985, 2013
BstV2I	GAAGAC	2 cut(s)	244, 621
BstX2I	RGATCY	2 cut(s)	1201, 1505
BstYI	RGATCY	2 cut(s)	1201, 1505
BsuI	GTATCC	2 cut(s)	534, 2065
BsuRI	GGCC	5 cut(s)	1299, 1595, 2049, 2149, 2250
BtgI	CCRYGG	1 cut(s)	1180
BtgZI	GCGATG	1 cut(s)	1800
BtsCI	GGATG	2 cut(s)	1246, 1393
BtsIMutI	CAGTG	5 cut(s)	603, 825, 963, 1163, 1994
BveI	ACCTGC	1 cut(s)	1825
Cac8I	GCNNGC	9 cut(s)	412, 435, 483, 536, 1720, 1885, 2051, 2244, 2248
CaiI	CAGNNNCTG	1 cut(s)	1205
CciI	TCATGA	1 cut(s)	1392
CfoI	GCGC	3 cut(s)	540, 1382, 1975
Cfr10I	RCCGGY	1 cut(s)	1299
Cfr13I	GGNCC	2 cut(s)	1814, 2123
CseI	GACGC	1 cut(s)	91
Csp6I	GTAC	4 cut(s)	1228, 1469, 2026, 2259
CspCI	CAANNNNNGTGG	4 cut(s)	474, 509, 1276, 1311
CviQI	GTAC	4 cut(s)	1228, 1469, 2026, 2259
DdeI	CTNAG	7 cut(s)	760, 1155, 1206, 1554, 1896, 2273, 2294
DpnI	GATC	8 cut(s)	705, 972, 1203, 1507, 1626, 2093, 2186, 2205
DpnII	GATC	8 cut(s)	703, 970, 1201, 1505, 1624, 2091, 2184, 2203
EaeI	YGGCCR	1 cut(s)	1297
Eam1104I	CTCTTC	6 cut(s)	783, 813, 970, 1106, 1495, 1735
EarI	CTCTTC	6 cut(s)	783, 813, 970, 1106, 1495, 1735
Eco130I	CCWWGG	3 cut(s)	594, 1180, 1231
Eco24I	GRGCYC	1 cut(s)	1024
Eco47I	GGWCC	2 cut(s)	1814, 2123
Eco57I	CTGAAG	1 cut(s)	1467
Eco88I	CYCGRG	1 cut(s)	1134
EcoNI	CCTNNNNNAGG	1 cut(s)	621
EcoRI	GAATTC	1 cut(s)	858
EcoRII	CCWGG	2 cut(s)	1491, 2103
EcoT14I	CCWWGG	3 cut(s)	594, 1180, 1231
EcoT22I	ATGCAT	1 cut(s)	1175
EcoT38I	GRGCYC	1 cut(s)	1024
ErhI	CCWWGG	3 cut(s)	594, 1180, 1231
Esp3I	CGTCTC	2 cut(s)	494, 1610
FaqI	GGGAC	2 cut(s)	1150, 1949
FauI	CCCGC	2 cut(s)	370, 2015
FauNDI	CATATG	1 cut(s)	1598
FblI	GTMKAC	1 cut(s)	274
Fnu4HI	GCNGC	8 cut(s)	438, 575, 589, 1265, 1909, 1999, 2002, 2131
FokI	GGATG	2 cut(s)	1253, 1400
FriOI	GRGCYC	1 cut(s)	1024
Fsp4HI	GCNGC	8 cut(s)	438, 575, 589, 1265, 1909, 1999, 2002, 2131
FspBI	CTAG	7 cut(s)	45, 248, 332, 941, 1760, 1860, 2151
GlaI	GCGC	3 cut(s)	539, 1381, 1974
GluI	GCNGC	8 cut(s)	438, 575, 589, 1265, 1909, 1999, 2002, 2131
GsuI	CTGGAG	3 cut(s)	774, 967, 1100
HaeIII	GGCC	5 cut(s)	1299, 1595, 2049, 2149, 2250
HapII	CCGG	6 cut(s)	16, 578, 633, 815, 1300, 1905
HgaI	GACGC	1 cut(s)	91
HhaI	GCGC	3 cut(s)	540, 1382, 1975
Hin6I	GCGC	3 cut(s)	538, 1380, 1973
HinP1I	GCGC	3 cut(s)	538, 1380, 1973
HincII	GTYRAC	3 cut(s)	90, 1417, 2140
HindII	GTYRAC	3 cut(s)	90, 1417, 2140
HindIII	AAGCTT	2 cut(s)	642, 882
HinfI	GANTC	6 cut(s)	40, 809, 953, 1856, 2196, 2271
HpaII	CCGG	6 cut(s)	16, 578, 633, 815, 1300, 1905
HphI	GGTGA	4 cut(s)	101, 665, 902, 1526
Hpy166II	GTNNAC	8 cut(s)	90, 217, 275, 1196, 1417, 1765, 1919, 2140
Hpy188I	TCNGA	6 cut(s)	1343, 1814, 1855, 1897, 2201, 2276
Hpy8I	GTNNAC	8 cut(s)	90, 217, 275, 1196, 1417, 1765, 1919, 2140
Hpy99I	CGWCG	2 cut(s)	554, 2290
HpyAV	CCTTC	6 cut(s)	110, 336, 724, 895, 1231, 2056
HpyCH4III	ACNGT	5 cut(s)	279, 607, 1769, 1940, 2144
HpyCH4IV	ACGT	1 cut(s)	127
HpyF3I	CTNAG	7 cut(s)	760, 1155, 1206, 1554, 1896, 2273, 2294
HpySE526I	ACGT	1 cut(s)	127
HspAI	GCGC	3 cut(s)	538, 1380, 1973
KpnI	GGTACC	1 cut(s)	1231
Kzo9I	GATC	8 cut(s)	703, 970, 1201, 1505, 1624, 2091, 2184, 2203
LguI	GCTCTTC	2 cut(s)	783, 1106
LmnI	GCTCC	5 cut(s)	554, 596, 689, 755, 2065
Lsp1109I	GCAGC	6 cut(s)	449, 561, 1276, 1895, 1985, 2013
LweI	GCATC	2 cut(s)	820, 1795
MaeI	CTAG	7 cut(s)	45, 248, 332, 941, 1760, 1860, 2151
MaeII	ACGT	1 cut(s)	127
MaeIII	GTNAC	8 cut(s)	128, 653, 839, 890, 1091, 1324, 1514, 2157
MalI	GATC	8 cut(s)	705, 972, 1203, 1507, 1626, 2093, 2186, 2205
MboI	GATC	8 cut(s)	703, 970, 1201, 1505, 1624, 2091, 2184, 2203
MflI	RGATCY	2 cut(s)	1201, 1505
MhlI	GDGCHC	5 cut(s)	605, 827, 1024, 1198, 1757
MluCI	AATT	5 cut(s)	676, 858, 1054, 1074, 1568
MlyI	GAGTC	3 cut(s)	962, 2190, 2265
MmeI	TCCRAC	1 cut(s)	1477
Mph1103I	ATGCAT	1 cut(s)	1175
MroXI	GAANNNNTTC	3 cut(s)	58, 72, 1055
MseI	TTAA	3 cut(s)	1545, 1611, 2112
MslI	CAYNNNNRTG	1 cut(s)	480
MspA1I	CMGCKG	2 cut(s)	2004, 2053
MspI	CCGG	6 cut(s)	16, 578, 633, 815, 1300, 1905
MspR9I	CCNGG	4 cut(s)	16, 815, 1493, 2105
Mva1269I	GAATGC	3 cut(s)	745, 1468, 1970
MvaI	CCWGG	2 cut(s)	1493, 2105
MvnI	CGCG	2 cut(s)	708, 1975
NciI	CCSGG	2 cut(s)	16, 815
NcoI	CCATGG	1 cut(s)	1180
NdeI	CATATG	1 cut(s)	1598
NdeII	GATC	8 cut(s)	703, 970, 1201, 1505, 1624, 2091, 2184, 2203
NlaIV	GGNNCC	5 cut(s)	61, 75, 592, 757, 1229
NmeAIII	GCCGAG	1 cut(s)	1408
NmuCI	GTSAC	4 cut(s)	653, 890, 1324, 1514
NsiI	ATGCAT	1 cut(s)	1175
NspI	RCATGY	3 cut(s)	485, 1887, 2231
PaeI	GCATGC	2 cut(s)	485, 1887
PagI	TCATGA	1 cut(s)	1392
PciSI	GCTCTTC	2 cut(s)	783, 1106
PctI	GAATGC	3 cut(s)	745, 1468, 1970
PdmI	GAANNNNTTC	3 cut(s)	58, 72, 1055
PfeI	GAWTC	3 cut(s)	40, 809, 1856
PflFI	GACNNNGTC	1 cut(s)	85
PkrI	GCNGC	8 cut(s)	439, 576, 590, 1266, 1910, 2000, 2003, 2132
Ple19I	CGATCG	1 cut(s)	973
PleI	GAGTC	3 cut(s)	961, 2190, 2265
PpsI	GAGTC	3 cut(s)	961, 2190, 2265
Psp6I	CCWGG	2 cut(s)	1491, 2103
PspGI	CCWGG	2 cut(s)	1491, 2103
PspN4I	GGNNCC	5 cut(s)	61, 75, 592, 757, 1229
PspPI	GGNCC	2 cut(s)	1814, 2123
PsrI	GAACNNNNNNTAC	4 cut(s)	43, 57, 75, 89
PstI	CTGCAG	1 cut(s)	2003
PstNI	CAGNNNCTG	1 cut(s)	1205
PsuI	RGATCY	2 cut(s)	1201, 1505
PsyI	GACNNNGTC	1 cut(s)	85
PvuI	CGATCG	1 cut(s)	973
PvuII	CAGCTG	2 cut(s)	2004, 2053
RsaI	GTAC	4 cut(s)	1229, 1470, 2027, 2260
RsaNI	GTAC	4 cut(s)	1228, 1469, 2026, 2259
RseI	CAYNNNNRTG	1 cut(s)	480
SapI	GCTCTTC	2 cut(s)	783, 1106
SaqAI	TTAA	3 cut(s)	1545, 1611, 2112
SatI	GCNGC	8 cut(s)	438, 575, 589, 1265, 1909, 1999, 2002, 2131
Sau3AI	GATC	8 cut(s)	703, 970, 1201, 1505, 1624, 2091, 2184, 2203
Sau96I	GGNCC	2 cut(s)	1814, 2123
ScaI	AGTACT	1 cut(s)	1470
SchI	GAGTC	3 cut(s)	962, 2190, 2265
ScrFI	CCNGG	4 cut(s)	16, 815, 1493, 2105
SduI	GDGCHC	5 cut(s)	605, 827, 1024, 1198, 1757
SfaNI	GCATC	2 cut(s)	820, 1795
SfcI	CTRYAG	1 cut(s)	1999
SinI	GGWCC	2 cut(s)	1814, 2123
SmiMI	CAYNNNNRTG	1 cut(s)	480
SmlI	CTYRAG	3 cut(s)	1258, 1652, 1670
SmoI	CTYRAG	3 cut(s)	1258, 1652, 1670
SphI	GCATGC	2 cut(s)	485, 1887
Sse9I	AATT	5 cut(s)	676, 858, 1054, 1074, 1568
SsiI	CCGC	6 cut(s)	363, 588, 708, 2022, 2130, 2266
SspMI	CTAG	7 cut(s)	45, 248, 332, 941, 1760, 1860, 2151
StyD4I	CCNGG	4 cut(s)	14, 813, 1491, 2103
StyI	CCWWGG	3 cut(s)	594, 1180, 1231
TaaI	ACNGT	5 cut(s)	279, 607, 1769, 1940, 2144
TaiI	ACGT	1 cut(s)	130
TaqI	TCGA	7 cut(s)	542, 671, 973, 1458, 1561, 2094, 2183
TasI	AATT	5 cut(s)	676, 858, 1054, 1074, 1568
TatI	WGTACW	2 cut(s)	1468, 2258
TauI	GCSGC	2 cut(s)	591, 2133
TfiI	GAWTC	3 cut(s)	40, 809, 1856
Tru1I	TTAA	3 cut(s)	1545, 1611, 2112
Tru9I	TTAA	3 cut(s)	1545, 1611, 2112
TscAI	CASTG	5 cut(s)	610, 832, 963, 1163, 1994
TseFI	GTSAC	4 cut(s)	653, 890, 1324, 1514
TseI	GCWGC	6 cut(s)	437, 574, 1264, 1908, 1998, 2001
Tsp45I	GTSAC	4 cut(s)	653, 890, 1324, 1514
TspDTI	ATGAA	8 cut(s)	279, 950, 1367, 1409, 1919, 1977, 2075, 2108
TspGWI	ACGGA	3 cut(s)	52, 567, 852
TspRI	CASTG	5 cut(s)	610, 832, 963, 1163, 1994
Tth111I	GACNNNGTC	1 cut(s)	85
VneI	GTGCAC	1 cut(s)	1194
VpaK11BI	GGWCC	2 cut(s)	1814, 2123
XagI	CCTNNNNNAGG	1 cut(s)	621
XapI	RAATTY	4 cut(s)	676, 858, 1054, 1074
XbaI	TCTAGA	1 cut(s)	1859
XceI	RCATGY	3 cut(s)	485, 1887, 2231
XmiI	GTMKAC	1 cut(s)	274
XmnI	GAANNNNTTC	3 cut(s)	58, 72, 1055
XspI	CTAG	7 cut(s)	45, 248, 332, 941, 1760, 1860, 2151
ZrmI	AGTACT	1 cut(s)	1470
Zsp2I	ATGCAT	1 cut(s)	1175

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.