MD05G1245300.v1.1

cationic amino acid transporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0013891
Physical Location & Seq
Reverse (-)
37734343 .. 37736197
1855 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1245300.v1.1.491

Sequence Viewer

Length: 1359 bp
ATGGGTTCCATGGAAGTGCGGGGTGAAGACGTCCAGCAAAGAAGTTACTGGAGATGCAGCAAACAAGATTTCCTACCAGAAGAATCCTTTCAGAATTGGAGTACATACCGAACGGCCCTGTCACAAACAGGCCACAGGTTCAAGAACCGTCTCCTCAGCAGATCAAATGATAACGATGAGATTGGGGAACTTCGGAAACAAAGTGAGAATGACATGAAGCGCTGCCTTACTTGGTGGGATCTCATGTGGTTTGGGTTTGGTTCGGTCATTGGTGCAGGCATCTTTGTGCTTACTGGCCTAGAAACTCAGAGACATGCTGGACCAGCTATTGTCTTGTCCTATGTTGCTTCCGGTCTCTCCGCAATGCTCTCTGTCTTCTGCTACACAGAATTTTCAATAGAAATCCCTGTGGCTGGTGGAGCTTTTGCTTACCTGCGGATAGAATTGGGGGACTTTGTTGCATTCATAACAGCAGGAAACATACTTCTTGAAAGCATTGTTGGAAGTGCAGGACTTGCTAGAGCATGGACTTCTTACTTCACAGCGCTCTTGAATCATCCTTCCAACTCGCTACGCATCCACACAAATCTGGCGGAGGGGTTCAATCTGCTGGACCCGATAGCTGTCACTGTTCTGGTAGCTGTTGGTGCCCTCAAGGGAATGACCACTGTTCTTCTGGTCGGGACACTTGGACAGGCACGATATATCACTCATATTGCACGAGCCCACATGATTCCACCATGGTTTGCTCTTGTTCATCCGAAGACAGGAACCCCCATAAACGCTACAGTTTTGATTGCCATTTCAAGTAGCTGCATCGCTTTCTTTTCGAGCTTGGAAGTTTTGACAGGCCTGTTATCAGTGAGCACGCTCTTTATCTTCATGATGATGGCAGTCGCACTTCTGGTGAGGAGGTACTATGTGAAAGATATCACCCCAAAACACCACCTCTTAAATCTTGTCATCTTCTTGCTGATCATTATTGCTTCCTCAATGGGGACTGCAGCTTACTGGGGGCTGAATCCCAATGGTTGGGTTGGCTATGTCGTGACAGTTCCTCTTTGGTTCTTTGGGACTTTGGTGATGGCAGTGTTTTTACCTCAACAACGGTCACCAAAACTTTGGGGGGTTCCGCTGGTACCTTGGTTGCCATCCCTGTCAATTGCAACAAACATCTTTCTTATGGGATCTTTGGGTCCTGAAGCTTTCGAAAGATTTGGAATCTGCACTGTAGTCATGCTTATTTACTACGTCTTCTTTGGTCTGCATGCAACTTACGATGTGGCTCATAAGCAAGACATGACAGAAGAATCCCTGAAAGTCAATGGCAGCAATATCAGAGAAAAGGCAGAGCCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AA_permease_2 AA_permease_C

Protein Analysis

453

Amino Acids

50.1

Weight (kDa)

7.71

Isoelectric Point (pI)

36.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AA_permease_2 PF13520 76 - 185 4.9e-11 Amino acid permease
AA_permease PF00324 81 - 168 6.6e-06 Amino acid permease
AA_permease_2 PF13520 208 - 332 6.8e-09 Amino acid permease
AA_permease_C PF13906 375 - 424 1.2e-17 C-terminus of AA_permease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013891)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G34960
fragaria_vesca FvH4_3g13060
malus_domestica MD05G1245200.v1.1 MD05G1245300.v1.1
prunus_persica Prupe.4G118600_v2.0.a1 Prupe.4G118700_v2.0.a1
pyrus_communis pycom10g19060
rosa_chinensis RchiOBHm_Chr5g0021251
rosa_laevigata RLG00000032599
rosa_multiflora Rmu_sc0004847.1_g000002
rosa_roxburghii Rroxscaffold_1G00057250
rosa_rugosa Rorug05G0066000
rosa_samantha Rh5AG154700 Rh5BG154200 Rh5CG167800 Rh5DG154800
rosa_wichuraiana Rw5G013790

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 33
Acc36I ACCTGC 1 cut(s) 441
Acc65I GGTACC 1 cut(s) 1138
AccB1I GGYRCC 2 cut(s) 647, 1138
AccB7I CCANNNNNTGG 1 cut(s) 1032
AciI CCGC 5 cut(s) 19, 360, 436, 593, 1133
AclWI GGATC 2 cut(s) 246, 1195
AcsI RAATTY 1 cut(s) 389
AcuI CTGAAG 1 cut(s) 1221
AcyI GRCGYC 1 cut(s) 30
AfaI GTAC 3 cut(s) 103, 917, 1140
AfeI AGCGCT 2 cut(s) 221, 546
AfiI CCNNNNNNNGG 4 cut(s) 413, 767, 996, 1032
AgsI TTSAA 6 cut(s) 142, 396, 491, 553, 604, 807
AjuI GAANNNNNNNTTGG 2 cut(s) 483, 515
AluBI AGCT 8 cut(s) 326, 422, 623, 641, 813, 834, 1007, 1205
AluI AGCT 8 cut(s) 326, 422, 623, 641, 813, 834, 1007, 1205
Alw21I GWGCWC 1 cut(s) 869
Alw26I GTCTC 3 cut(s) 155, 304, 359
AlwI GGATC 2 cut(s) 246, 1195
Aor51HI AGCGCT 2 cut(s) 221, 546
AoxI GGCC 4 cut(s) 114, 130, 295, 850
ApeKI GCWGC 5 cut(s) 57, 222, 813, 1004, 1329
ApoI RAATTY 1 cut(s) 389
Asp700I GAANNNNTTC 1 cut(s) 87
Asp718I GGTACC 1 cut(s) 1138
AspLEI GCGC 2 cut(s) 222, 547
AspS9I GGNCC 4 cut(s) 115, 320, 613, 1196
AsuHPI GGTGA 5 cut(s) 35, 919, 925, 1093, 1104
AsuII TTCGAA 1 cut(s) 1209
AvaII GGWCC 3 cut(s) 320, 613, 1196
BaeGI GKGCMC 1 cut(s) 652
BaeI ACNNNNGTAYC 2 cut(s) 1130, 1163
BanI GGYRCC 2 cut(s) 647, 1138
BanII GRGCYC 1 cut(s) 727
BauI CACGAG 1 cut(s) 720
BbsI GAAGAC 4 cut(s) 33, 367, 770, 1246
Bbv12I GWGCWC 1 cut(s) 869
BbvCI CCTCAGC 1 cut(s) 155
BbvI GCAGC 5 cut(s) 69, 209, 800, 1016, 1341
BccI CCATC 3 cut(s) 883, 1078, 1159
BceAI ACGGC 1 cut(s) 129
BclI TGATCA 1 cut(s) 975
BcoDI GTCTC 3 cut(s) 155, 304, 359
BfaI CTAG 2 cut(s) 299, 519
BfmI CTRYAG 3 cut(s) 786, 1002, 1230
BfoI RGCGCY 2 cut(s) 223, 548
BfuAI ACCTGC 1 cut(s) 441
BisI GCNGC 5 cut(s) 58, 223, 814, 1005, 1330
BlsI GCNGC 5 cut(s) 59, 224, 815, 1006, 1331
Bme18I GGWCC 3 cut(s) 320, 613, 1196
BmgT120I GGNCC 4 cut(s) 115, 320, 613, 1196
BmiI GGNNCC 7 cut(s) 7, 615, 649, 772, 1131, 1140, 1197
BmrI ACTGGG 1 cut(s) 1021
BmsI GCATC 4 cut(s) 44, 288, 585, 825
BmuI ACTGGG 1 cut(s) 1021
BpiI GAAGAC 4 cut(s) 33, 367, 770, 1246
BpmI CTGGAG 1 cut(s) 70
Bpu10I CCTNAGC 1 cut(s) 155
Bpu14I TTCGAA 1 cut(s) 1209
BpuEI CTTGAG 1 cut(s) 638
BsaHI GRCGYC 1 cut(s) 30
BsaI GGTCTC 1 cut(s) 359
BsaJI CCNNGG 3 cut(s) 9, 740, 1142
BsaWI WCCGGW 1 cut(s) 350
Bsc4I CCNNNNNNNGG 4 cut(s) 413, 767, 996, 1032
Bse1I ACTGG 3 cut(s) 53, 298, 1016
Bse3DI GCAATG 1 cut(s) 369
BseDI CCNNGG 3 cut(s) 9, 740, 1142
BseGI GGATG 4 cut(s) 556, 576, 757, 1151
BseLI CCNNNNNNNGG 4 cut(s) 413, 767, 996, 1032
BseMI GCAATG 1 cut(s) 369
BseMII CTCAG 2 cut(s) 169, 320
BseNI ACTGG 3 cut(s) 53, 298, 1016
BseRI GAGGAG 2 cut(s) 143, 925
BseSI GKGCMC 1 cut(s) 652
BseXI GCAGC 5 cut(s) 69, 209, 800, 1016, 1341
BsgI GTGCAG 3 cut(s) 294, 528, 1210
BshFI GGCC 4 cut(s) 116, 132, 297, 852
BshNI GGYRCC 2 cut(s) 647, 1138
BsiHKAI GWGCWC 1 cut(s) 869
BsiSI CCGG 1 cut(s) 351
BslFI GGGAC 4 cut(s) 464, 697, 1012, 1087
BslI CCNNNNNNNGG 4 cut(s) 413, 767, 996, 1032
BsmAI GTCTC 3 cut(s) 155, 304, 359
BsmBI CGTCTC 1 cut(s) 155
BsmFI GGGAC 4 cut(s) 464, 697, 1012, 1087
BsmI GAATGC 1 cut(s) 461
BsnI GGCC 4 cut(s) 116, 132, 297, 852
Bso31I GGTCTC 1 cut(s) 359
Bsp119I TTCGAA 1 cut(s) 1209
Bsp1286I GDGCHC 3 cut(s) 652, 727, 869
Bsp143I GATC 4 cut(s) 161, 238, 975, 1187
Bsp19I CCATGG 2 cut(s) 9, 740
BspACI CCGC 5 cut(s) 19, 360, 436, 593, 1133
BspANI GGCC 4 cut(s) 116, 132, 297, 852
BspCNI CTCAG 2 cut(s) 168, 319
BspHI TCATGA 1 cut(s) 882
BspLI GGNNCC 7 cut(s) 7, 615, 649, 772, 1131, 1140, 1197
BspMAI CTGCAG 1 cut(s) 1006
BspMI ACCTGC 1 cut(s) 441
BspPI GGATC 2 cut(s) 246, 1195
BspT104I TTCGAA 1 cut(s) 1209
BspT107I GGYRCC 2 cut(s) 647, 1138
BspTNI GGTCTC 1 cut(s) 359
BsrDI GCAATG 1 cut(s) 369
BsrI ACTGG 3 cut(s) 53, 298, 1016
BssECI CCNNGG 3 cut(s) 9, 740, 1142
BssMI GATC 4 cut(s) 161, 238, 975, 1187
BssNI GRCGYC 1 cut(s) 30
BssSI CACGAG 1 cut(s) 720
BssT1I CCWWGG 3 cut(s) 9, 740, 1142
Bst2BI CACGAG 1 cut(s) 720
Bst4CI ACNGT 7 cut(s) 149, 631, 670, 790, 1054, 1110, 1231
BstACI GRCGYC 1 cut(s) 30
BstAPI GCANNNNNTGC 1 cut(s) 515
BstBI TTCGAA 1 cut(s) 1209
BstC8I GCNNGC 3 cut(s) 277, 869, 1269
BstDEI CTNAG 2 cut(s) 155, 306
BstDSI CCRYGG 2 cut(s) 9, 740
BstEII GGTNACC 1 cut(s) 1110
BstF5I GGATG 4 cut(s) 556, 576, 757, 1151
BstH2I RGCGCY 2 cut(s) 223, 548
BstHHI GCGC 2 cut(s) 222, 547
BstKTI GATC 4 cut(s) 164, 241, 978, 1190
BstMAI GTCTC 3 cut(s) 155, 304, 359
BstMBI GATC 4 cut(s) 161, 238, 975, 1187
BstMWI GCNNNNNNNGC 4 cut(s) 323, 419, 515, 647
BstNSI RCATGY 2 cut(s) 317, 1271
BstPI GGTNACC 1 cut(s) 1110
BstSFI CTRYAG 3 cut(s) 786, 1002, 1230
BstSLI GKGCMC 1 cut(s) 652
BstV1I GCAGC 5 cut(s) 69, 209, 800, 1016, 1341
BstV2I GAAGAC 4 cut(s) 33, 367, 770, 1246
BstX2I RGATCY 2 cut(s) 238, 1187
BstXI CCANNNNNNTGG 1 cut(s) 1122
BstYI RGATCY 2 cut(s) 238, 1187
BsuRI GGCC 4 cut(s) 116, 132, 297, 852
BtgI CCRYGG 2 cut(s) 9, 740
BtgZI GCGATG 1 cut(s) 802
BtsCI GGATG 4 cut(s) 556, 576, 757, 1151
BtsI GCAGTG 1 cut(s) 1095
BtsIMutI CAGTG 5 cut(s) 627, 666, 867, 1095, 1227
BveI ACCTGC 1 cut(s) 441
Cac8I GCNNGC 3 cut(s) 277, 869, 1269
CciI TCATGA 1 cut(s) 882
CfoI GCGC 2 cut(s) 222, 547
Cfr13I GGNCC 4 cut(s) 115, 320, 613, 1196
Csp6I GTAC 3 cut(s) 102, 916, 1139
CviQI GTAC 3 cut(s) 102, 916, 1139
DdeI CTNAG 2 cut(s) 155, 306
DpnI GATC 4 cut(s) 163, 240, 977, 1189
DpnII GATC 4 cut(s) 161, 238, 975, 1187
EciI GGCGGA 1 cut(s) 608
Eco130I CCWWGG 3 cut(s) 9, 740, 1142
Eco147I AGGCCT 1 cut(s) 852
Eco24I GRGCYC 1 cut(s) 727
Eco31I GGTCTC 1 cut(s) 359
Eco32I GATATC 1 cut(s) 931
Eco47I GGWCC 3 cut(s) 320, 613, 1196
Eco47III AGCGCT 2 cut(s) 221, 546
Eco57I CTGAAG 1 cut(s) 1221
Eco91I GGTNACC 1 cut(s) 1110
EcoO109I RGGNCCY 1 cut(s) 1196
EcoO65I GGTNACC 1 cut(s) 1110
EcoRV GATATC 1 cut(s) 931
EcoT14I CCWWGG 3 cut(s) 9, 740, 1142
EcoT38I GRGCYC 1 cut(s) 727
ErhI CCWWGG 3 cut(s) 9, 740, 1142
Esp3I CGTCTC 1 cut(s) 155
FaqI GGGAC 4 cut(s) 464, 697, 1012, 1087
FauI CCCGC 1 cut(s) 12
FbaI TGATCA 1 cut(s) 975
Fnu4HI GCNGC 5 cut(s) 58, 223, 814, 1005, 1330
FokI GGATG 4 cut(s) 543, 563, 744, 1138
FriOI GRGCYC 1 cut(s) 727
Fsp4HI GCNGC 5 cut(s) 58, 223, 814, 1005, 1330
FspBI CTAG 2 cut(s) 299, 519
GlaI GCGC 2 cut(s) 221, 546
GluI GCNGC 5 cut(s) 58, 223, 814, 1005, 1330
GsuI CTGGAG 1 cut(s) 70
HaeII RGCGCY 2 cut(s) 223, 548
HaeIII GGCC 4 cut(s) 116, 132, 297, 852
HapII CCGG 1 cut(s) 351
HhaI GCGC 2 cut(s) 222, 547
Hin1I GRCGYC 1 cut(s) 30
Hin6I GCGC 2 cut(s) 220, 545
HinP1I GCGC 2 cut(s) 220, 545
HindIII AAGCTT 1 cut(s) 1203
HinfI GANTC 6 cut(s) 83, 553, 733, 1021, 1221, 1310
HpaII CCGG 1 cut(s) 351
HphI GGTGA 5 cut(s) 35, 919, 925, 1093, 1104
Hpy188I TCNGA 5 cut(s) 93, 195, 309, 762, 1340
Hpy188III TCNNGA 7 cut(s) 142, 488, 550, 682, 883, 1048, 1199
HpyAV CCTTC 1 cut(s) 570
HpyCH4III ACNGT 7 cut(s) 149, 631, 670, 790, 1054, 1110, 1231
HpyCH4IV ACGT 2 cut(s) 30, 1251
HpyF10VI GCNNNNNNNGC 4 cut(s) 323, 419, 515, 647
HpyF3I CTNAG 2 cut(s) 155, 306
HpySE526I ACGT 2 cut(s) 30, 1251
Hsp92I GRCGYC 1 cut(s) 30
HspAI GCGC 2 cut(s) 220, 545
KpnI GGTACC 1 cut(s) 1142
Ksp22I TGATCA 1 cut(s) 975
Kzo9I GATC 4 cut(s) 161, 238, 975, 1187
LmnI GCTCC 1 cut(s) 419
Lsp1109I GCAGC 5 cut(s) 69, 209, 800, 1016, 1341
LweI GCATC 4 cut(s) 44, 288, 585, 825
MaeI CTAG 2 cut(s) 299, 519
MaeII ACGT 2 cut(s) 30, 1251
MaeIII GTNAC 5 cut(s) 44, 120, 625, 1048, 1110
MalI GATC 4 cut(s) 163, 240, 977, 1189
MboI GATC 4 cut(s) 161, 238, 975, 1187
MboII GAAGA 9 cut(s) 38, 92, 367, 665, 775, 871, 958, 1246, 1319
MfeI CAATTG 1 cut(s) 1161
MflI RGATCY 2 cut(s) 238, 1187
MhlI GDGCHC 3 cut(s) 652, 727, 869
MluCI AATT 4 cut(s) 94, 389, 443, 1161
MmeI TCCRAC 2 cut(s) 481, 588
MnlI CCTC 9 cut(s) 164, 589, 662, 903, 906, 959, 1000, 1068, 1110
MroXI GAANNNNTTC 1 cut(s) 87
MseI TTAA 1 cut(s) 953
MslI CAYNNNNRTG 3 cut(s) 14, 284, 887
MspA1I CMGCKG 1 cut(s) 1135
MspI CCGG 1 cut(s) 351
MunI CAATTG 1 cut(s) 1161
Mva1269I GAATGC 1 cut(s) 461
MwoI GCNNNNNNNGC 4 cut(s) 323, 419, 515, 647
NcoI CCATGG 2 cut(s) 9, 740
NdeII GATC 4 cut(s) 161, 238, 975, 1187
NlaIV GGNNCC 7 cut(s) 7, 615, 649, 772, 1131, 1140, 1197
NmuCI GTSAC 4 cut(s) 120, 625, 1048, 1110
NspI RCATGY 2 cut(s) 317, 1271
NspV TTCGAA 1 cut(s) 1209
PaeI GCATGC 1 cut(s) 1271
PagI TCATGA 1 cut(s) 882
PceI AGGCCT 1 cut(s) 852
PctI GAATGC 1 cut(s) 461
PdmI GAANNNNTTC 1 cut(s) 87
PfeI GAWTC 6 cut(s) 83, 553, 733, 1021, 1221, 1310
PflMI CCANNNNNTGG 1 cut(s) 1032
PkrI GCNGC 5 cut(s) 59, 224, 815, 1006, 1331
PpuMI RGGWCCY 1 cut(s) 1196
Psp5II RGGWCCY 1 cut(s) 1196
PspEI GGTNACC 1 cut(s) 1110
PspN4I GGNNCC 7 cut(s) 7, 615, 649, 772, 1131, 1140, 1197
PspPI GGNCC 4 cut(s) 115, 320, 613, 1196
PspPPI RGGWCCY 1 cut(s) 1196
PstI CTGCAG 1 cut(s) 1006
PsuI RGATCY 2 cut(s) 238, 1187
RsaI GTAC 3 cut(s) 103, 917, 1140
RsaNI GTAC 3 cut(s) 102, 916, 1139
RseI CAYNNNNRTG 3 cut(s) 14, 284, 887
SaqAI TTAA 1 cut(s) 953
SatI GCNGC 5 cut(s) 58, 223, 814, 1005, 1330
Sau3AI GATC 4 cut(s) 161, 238, 975, 1187
Sau96I GGNCC 4 cut(s) 115, 320, 613, 1196
SduI GDGCHC 3 cut(s) 652, 727, 869
SfaNI GCATC 4 cut(s) 44, 288, 585, 825
SfcI CTRYAG 3 cut(s) 786, 1002, 1230
SfuI TTCGAA 1 cut(s) 1209
SinI GGWCC 3 cut(s) 320, 613, 1196
SmiMI CAYNNNNRTG 3 cut(s) 14, 284, 887
SmlI CTYRAG 1 cut(s) 653
SmoI CTYRAG 1 cut(s) 653
SphI GCATGC 1 cut(s) 1271
Sse9I AATT 4 cut(s) 94, 389, 443, 1161
SseBI AGGCCT 1 cut(s) 852
SsiI CCGC 5 cut(s) 19, 360, 436, 593, 1133
SspMI CTAG 2 cut(s) 299, 519
StuI AGGCCT 1 cut(s) 852
StyI CCWWGG 3 cut(s) 9, 740, 1142
TaaI ACNGT 7 cut(s) 149, 631, 670, 790, 1054, 1110, 1231
TaiI ACGT 2 cut(s) 33, 1254
TaqI TCGA 2 cut(s) 830, 1209
TaqII GACCGA 1 cut(s) 253
TasI AATT 4 cut(s) 94, 389, 443, 1161
TatI WGTACW 1 cut(s) 101
TfiI GAWTC 6 cut(s) 83, 553, 733, 1021, 1221, 1310
Tru1I TTAA 1 cut(s) 953
Tru9I TTAA 1 cut(s) 953
TscAI CASTG 5 cut(s) 634, 673, 867, 1095, 1234
TseFI GTSAC 4 cut(s) 120, 625, 1048, 1110
TseI GCWGC 5 cut(s) 57, 222, 813, 1004, 1329
Tsp45I GTSAC 4 cut(s) 120, 625, 1048, 1110
TspDTI ATGAA 4 cut(s) 230, 454, 746, 871
TspRI CASTG 5 cut(s) 634, 673, 867, 1095, 1234
Van91I CCANNNNNTGG 1 cut(s) 1032
VpaK11BI GGWCC 3 cut(s) 320, 613, 1196
XapI RAATTY 1 cut(s) 389
XceI RCATGY 2 cut(s) 317, 1271
XcmI CCANNNNNNNNNTGG 1 cut(s) 673
XmnI GAANNNNTTC 1 cut(s) 87
XspI CTAG 2 cut(s) 299, 519
ZraI GACGTC 1 cut(s) 31
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.