Rroxscaffold_1G00057250

cationic amino acid transporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0013891
Physical Location & Seq
Reverse (-)
79225942 .. 79228241
2300 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00057250.1

Sequence Viewer

Length: 1653 bp
ATGGGTTCCGGGGGAGAAATTGGTGAAGAAGTCGAGCCCAGAACCTACTGGAGATGGAGCAAACAAGATTTCCTGCCAGAAGAGTCCTTTCAAAATTGGAGCGCATATCGAACTGCCTTGTCACAGACGCGCCACAGGTTCAAGGACCGACTCATAAGCAGATCAAATGATGAAAATGAGACTAGGGAGCTCCCGAAACAAAGTGAGAATAACATGAAACGTTGCCTCACATGGTGGGATCTCATCTGGTTTGGTTTCGGTTCAGTAATTGGTGCAGGCATCTTTGTGCTTACTGGGCAAGAAACGCATAACCATGCTGGACCAGCTATTGTCTTGTCCTATGTTGCTTCAGGTATCTCGGCAATGCTCTCTGTCTTCTGCTACACTGAATTTTCAGTCGAAATACCAGCGGCTGGTGGATCGTTTGCTTACCTCCGGATTGAGTTAGGAGACTTTGTAGCATTCATAACAGCAGGAAATATACTTCTTGAGGGTGTTGTAGGAAGTGCAGCAGTTGCCAGAGCATGGACTTCTTACTTCACATCACTCTTGAATCGTCCCTCCAACTCATTACGCTTCCACACAAATCTCACTGAGGGCTACAATCTCCTGGACCCAATAGCTGTTGTTGTTCTGACTGTAGCTGCTACAATTACAATGATCAGCACAAGGAAAACCTCTTACTTGAATTGGATAGCATCCGCAGTCAATAATGTTGTAATATTGTTTGTATTGATTGCAGGGTTTTCCCAAGCTAATCTCTCAAATATGAAACCTTTTATGCCTTTTGGGCCTAAAGGGGTCTTTCAAGCAGCTGCAATTGTTTACTTTGCTTACGGAGGATTTGACAACATTGCCACCATGGCTGAAGAAACCAAAAATCCATCCAGAGACATACCGATTGGATTGCTTGGATCAATGTCAATCATAACTGTTGTATATTGTTTAATGGCACTTTCACTGCCTATGATGCAGAAATACACAGACATAGACCCAAATGCAGCTTACTCTACTGCATTTCAAAGTGTGGGCATGACATGGGCCAAGTACCTGGTAGCTCTTGGTGCTCTCAAGGGAATGACCACTGTTCTTCTGGTGGGAACACTTGGACAGGCTCGGTATATCACTCACATAGCACGAGCCCACATGATTCCACCATGGTTTGCTCTTGTCCATCCAAAGACAGGAACCCCCATAAATGCTACACTCATGATAGTTGTTTCAAGTTCCTGGATTGCTTTCTTTTCAAGCTTGGACGTCTTGACAAGCCTGTTATCGGTGAGCACACTCTTTATCTTCACGTTGATGGCTGTTGCACTTCTAGTGAGGAGATACTACGTGACCGATGTCACCCCAAAACCACATCTCTTGAAGCTAGTTGTTTACTTGGTGATCATTATTTCTTCCTCAATAGGGACTTCAGCTTACTGGGGATTGAATCCCAATGGTTGGATTGGTTATGTTGTGACAGTTCCTCTTTGGTTCTTTGCAACTTTGGCAATGTCAGTGTTTCTACCTCAACAGAGGTCACCAAAAGTCTGGGGGTTCCACTGGTTCCTTGGTTGCCATCCTTGTCAATTGCCACAAATATTTTTCTCATGGGATCTTTGGGTTCTTCTGCTTTTGAAAGATTTGGAATATGCACTGTTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AA_permease_2 AA_permease_C

Protein Analysis

550

Amino Acids

61.09

Weight (kDa)

8.44

Isoelectric Point (pI)

32.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AA_permease_2 PF13520 76 - 481 4.8e-45 Amino acid permease
AA_permease PF00324 81 - 468 1.6e-27 Amino acid permease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013891)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G34960
fragaria_vesca FvH4_3g13060
malus_domestica MD05G1245200.v1.1 MD05G1245300.v1.1
prunus_persica Prupe.4G118600_v2.0.a1 Prupe.4G118700_v2.0.a1
pyrus_communis pycom10g19060
rosa_chinensis RchiOBHm_Chr5g0021251
rosa_laevigata RLG00000032599
rosa_multiflora Rmu_sc0004847.1_g000002
rosa_roxburghii Rroxscaffold_1G00057250
rosa_rugosa Rorug05G0066000
rosa_samantha Rh5AG154700 Rh5BG154200 Rh5CG167800 Rh5DG154800
rosa_wichuraiana Rw5G013790

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1260
AccB7I CCANNNNNTGG 3 cut(s) 413, 525, 1449
AccII CGCG 1 cut(s) 130
AccIII TCCGGA 1 cut(s) 435
AciI CCGC 2 cut(s) 410, 702
AclI AACGTT 1 cut(s) 220
AclWI GGATC 4 cut(s) 246, 427, 922, 1611
AcsI RAATTY 1 cut(s) 389
AcuI CTGAAG 3 cut(s) 333, 888, 1404
AcyI GRCGYC 1 cut(s) 1257
AdeI CACNNNGTG 1 cut(s) 234
AfaI GTAC 1 cut(s) 1049
AfiI CCNNNNNNNGG 6 cut(s) 413, 525, 1184, 1276, 1413, 1449
AjnI CCWGG 3 cut(s) 609, 1050, 1229
AleI CACNNNNGTG 1 cut(s) 1648
Alw21I GWGCWC 3 cut(s) 192, 1069, 1286
Alw26I GTCTC 3 cut(s) 173, 444, 885
AlwI GGATC 4 cut(s) 246, 427, 922, 1611
AlwNI CAGNNNCTG 1 cut(s) 413
Aor13HI TCCGGA 1 cut(s) 435
AoxI GGCC 2 cut(s) 791, 1041
ApeKI GCWGC 5 cut(s) 509, 644, 812, 815, 1001
ApoI RAATTY 1 cut(s) 389
AspLEI GCGC 2 cut(s) 104, 132
AspS9I GGNCC 5 cut(s) 145, 320, 613, 791, 1041
AsuC2I CCSGG 1 cut(s) 10
AsuHPI GGTGA 5 cut(s) 35, 1291, 1342, 1402, 1521
AvaII GGWCC 3 cut(s) 145, 320, 613
BanII GRGCYC 3 cut(s) 39, 192, 1144
BauI CACGAG 1 cut(s) 1137
BbsI GAAGAC 1 cut(s) 367
Bbv12I GWGCWC 3 cut(s) 192, 1069, 1286
BbvI GCAGC 5 cut(s) 521, 631, 802, 824, 1013
BccI CCATC 5 cut(s) 48, 892, 1182, 1300, 1575
BcgI CGANNNNNNTGC 2 cut(s) 889, 923
BciT130I CCWGG 3 cut(s) 611, 1052, 1231
BclI TGATCA 2 cut(s) 660, 1392
BcnI CCSGG 1 cut(s) 10
BcoDI GTCTC 3 cut(s) 173, 444, 885
BfaI CTAG 3 cut(s) 183, 1322, 1376
BfmI CTRYAG 1 cut(s) 639
BglI GCCNNNNNGGC 2 cut(s) 790, 863
BisI GCNGC 6 cut(s) 411, 510, 645, 813, 816, 1002
BlsI GCNGC 6 cut(s) 412, 511, 646, 814, 817, 1003
Bme1390I CCNGG 4 cut(s) 10, 611, 1052, 1231
Bme18I GGWCC 3 cut(s) 145, 320, 613
BmgT120I GGNCC 5 cut(s) 145, 320, 613, 791, 1041
BmiI GGNNCC 5 cut(s) 7, 615, 1189, 1547, 1556
BmrFI CCNGG 4 cut(s) 10, 611, 1052, 1231
BmrI ACTGGG 2 cut(s) 303, 1438
BmsI GCATC 3 cut(s) 288, 707, 960
BmuI ACTGGG 2 cut(s) 303, 1438
BoxI GACNNNNGTC 1 cut(s) 1346
BpiI GAAGAC 1 cut(s) 367
BpmI CTGGAG 1 cut(s) 70
BpuEI CTTGAG 2 cut(s) 509, 1055
BpuMI CCSGG 1 cut(s) 10
BsaAI YACGTR 1 cut(s) 1339
BsaHI GRCGYC 1 cut(s) 1257
BsaJI CCNNGG 4 cut(s) 9, 861, 1157, 1558
BsaWI WCCGGW 1 cut(s) 435
BsaXI ACNNNNNCTCC 2 cut(s) 441, 471
Bsc4I CCNNNNNNNGG 6 cut(s) 413, 525, 1184, 1276, 1413, 1449
Bse1I ACTGG 4 cut(s) 53, 298, 1433, 1556
Bse3DI GCAATG 3 cut(s) 369, 852, 1506
BseAI TCCGGA 1 cut(s) 435
BseBI CCWGG 3 cut(s) 611, 1052, 1231
BseDI CCNNGG 4 cut(s) 9, 861, 1157, 1558
BseGI GGATG 4 cut(s) 698, 884, 1174, 1567
BseLI CCNNNNNNNGG 6 cut(s) 413, 525, 1184, 1276, 1413, 1449
BseMI GCAATG 3 cut(s) 369, 852, 1506
BseMII CTCAG 1 cut(s) 585
BseNI ACTGG 4 cut(s) 53, 298, 1433, 1556
BseRI GAGGAG 1 cut(s) 1342
BseXI GCAGC 5 cut(s) 521, 631, 802, 824, 1013
BsgI GTGCAG 2 cut(s) 294, 528
Bsh1236I CGCG 1 cut(s) 130
BshFI GGCC 2 cut(s) 793, 1043
BsiHKAI GWGCWC 3 cut(s) 192, 1069, 1286
BsiSI CCGG 2 cut(s) 9, 436
BslFI GGGAC 2 cut(s) 543, 1429
BslI CCNNNNNNNGG 6 cut(s) 413, 525, 1184, 1276, 1413, 1449
BsmAI GTCTC 3 cut(s) 173, 444, 885
BsmFI GGGAC 2 cut(s) 543, 1429
BsmI GAATGC 1 cut(s) 461
BsnI GGCC 2 cut(s) 793, 1043
Bsp1286I GDGCHC 5 cut(s) 39, 192, 1069, 1144, 1286
Bsp13I TCCGGA 1 cut(s) 435
Bsp143I GATC 7 cut(s) 161, 238, 419, 660, 914, 1392, 1603
Bsp19I CCATGG 2 cut(s) 861, 1157
BspACI CCGC 2 cut(s) 410, 702
BspANI GGCC 2 cut(s) 793, 1043
BspCNI CTCAG 1 cut(s) 586
BspEI TCCGGA 1 cut(s) 435
BspFNI CGCG 1 cut(s) 130
BspHI TCATGA 1 cut(s) 1209
BspLI GGNNCC 5 cut(s) 7, 615, 1189, 1547, 1556
BspPI GGATC 4 cut(s) 246, 427, 922, 1611
BsrDI GCAATG 3 cut(s) 369, 852, 1506
BsrI ACTGG 4 cut(s) 53, 298, 1433, 1556
BssECI CCNNGG 4 cut(s) 9, 861, 1157, 1558
BssMI GATC 7 cut(s) 161, 238, 419, 660, 914, 1392, 1603
BssNI GRCGYC 1 cut(s) 1257
BssSI CACGAG 1 cut(s) 1137
BssT1I CCWWGG 3 cut(s) 861, 1157, 1558
Bst2BI CACGAG 1 cut(s) 1137
Bst2UI CCWGG 3 cut(s) 611, 1052, 1231
Bst4CI ACNGT 5 cut(s) 640, 934, 1087, 1471, 1647
Bst6I CTCTTC 1 cut(s) 75
BstACI GRCGYC 1 cut(s) 1257
BstAPI GCANNNNNTGC 1 cut(s) 515
BstBAI YACGTR 1 cut(s) 1339
BstC8I GCNNGC 1 cut(s) 277
BstDEI CTNAG 1 cut(s) 594
BstDSI CCRYGG 2 cut(s) 861, 1157
BstEII GGTNACC 1 cut(s) 1527
BstF5I GGATG 4 cut(s) 698, 884, 1174, 1567
BstFNI CGCG 1 cut(s) 130
BstHHI GCGC 2 cut(s) 104, 132
BstKTI GATC 7 cut(s) 164, 241, 422, 663, 917, 1395, 1606
BstMAI GTCTC 3 cut(s) 173, 444, 885
BstMBI GATC 7 cut(s) 161, 238, 419, 660, 914, 1392, 1603
BstMWI GCNNNNNNNGC 9 cut(s) 295, 304, 323, 515, 790, 863, 970, 1064, 1496
BstNI CCWGG 3 cut(s) 611, 1052, 1231
BstPAI GACNNNNGTC 1 cut(s) 1346
BstPI GGTNACC 1 cut(s) 1527
BstSCI CCNGG 4 cut(s) 8, 609, 1050, 1229
BstSFI CTRYAG 1 cut(s) 639
BstUI CGCG 1 cut(s) 130
BstV1I GCAGC 5 cut(s) 521, 631, 802, 824, 1013
BstV2I GAAGAC 1 cut(s) 367
BstX2I RGATCY 2 cut(s) 238, 1603
BstXI CCANNNNNNTGG 2 cut(s) 1051, 1539
BstYI RGATCY 2 cut(s) 238, 1603
BsuRI GGCC 2 cut(s) 793, 1043
BtgI CCRYGG 2 cut(s) 861, 1157
BtsCI GGATG 4 cut(s) 698, 884, 1174, 1567
BtsI GCAGTG 1 cut(s) 959
BtsIMutI CAGTG 7 cut(s) 384, 591, 959, 1083, 1512, 1549, 1643
Cac8I GCNNGC 1 cut(s) 277
CaiI CAGNNNCTG 1 cut(s) 413
CciI TCATGA 1 cut(s) 1209
CfoI GCGC 2 cut(s) 104, 132
Cfr13I GGNCC 5 cut(s) 145, 320, 613, 791, 1041
CseI GACGC 1 cut(s) 136
CsiI ACCWGGT 1 cut(s) 1050
Csp6I GTAC 1 cut(s) 1048
CspCI CAANNNNNGTGG 2 cut(s) 1350, 1385
CviQI GTAC 1 cut(s) 1048
DdeI CTNAG 1 cut(s) 594
DpnI GATC 7 cut(s) 163, 240, 421, 662, 916, 1394, 1605
DpnII GATC 7 cut(s) 161, 238, 419, 660, 914, 1392, 1603
DraIII CACNNNGTG 1 cut(s) 234
Eam1104I CTCTTC 1 cut(s) 75
EarI CTCTTC 1 cut(s) 75
Ecl136II GAGCTC 1 cut(s) 190
Eco130I CCWWGG 3 cut(s) 861, 1157, 1558
Eco24I GRGCYC 3 cut(s) 39, 192, 1144
Eco47I GGWCC 3 cut(s) 145, 320, 613
Eco53kI GAGCTC 1 cut(s) 190
Eco57I CTGAAG 3 cut(s) 333, 888, 1404
Eco91I GGTNACC 1 cut(s) 1527
EcoICRI GAGCTC 1 cut(s) 190
EcoO65I GGTNACC 1 cut(s) 1527
EcoRII CCWGG 3 cut(s) 609, 1050, 1229
EcoT14I CCWWGG 3 cut(s) 861, 1157, 1558
EcoT38I GRGCYC 3 cut(s) 39, 192, 1144
ErhI CCWWGG 3 cut(s) 861, 1157, 1558
FaqI GGGAC 2 cut(s) 543, 1429
FbaI TGATCA 2 cut(s) 660, 1392
Fnu4HI GCNGC 6 cut(s) 411, 510, 645, 813, 816, 1002
FokI GGATG 4 cut(s) 685, 871, 1161, 1554
FriOI GRGCYC 3 cut(s) 39, 192, 1144
Fsp4HI GCNGC 6 cut(s) 411, 510, 645, 813, 816, 1002
FspBI CTAG 3 cut(s) 183, 1322, 1376
GlaI GCGC 2 cut(s) 103, 131
GluI GCNGC 6 cut(s) 411, 510, 645, 813, 816, 1002
GsuI CTGGAG 1 cut(s) 70
HaeIII GGCC 2 cut(s) 793, 1043
HapII CCGG 2 cut(s) 9, 436
HgaI GACGC 1 cut(s) 136
HhaI GCGC 2 cut(s) 104, 132
Hin1I GRCGYC 1 cut(s) 1257
Hin6I GCGC 2 cut(s) 102, 130
HinP1I GCGC 2 cut(s) 102, 130
HindIII AAGCTT 1 cut(s) 1249
HinfI GANTC 5 cut(s) 83, 150, 553, 1150, 1438
HpaII CCGG 2 cut(s) 9, 436
HphI GGTGA 5 cut(s) 35, 1291, 1342, 1402, 1521
Hpy166II GTNNAC 2 cut(s) 826, 1384
Hpy188I TCNGA 1 cut(s) 636
Hpy188III TCNNGA 8 cut(s) 193, 436, 488, 550, 888, 1210, 1261, 1369
Hpy8I GTNNAC 2 cut(s) 826, 1384
HpyCH4III ACNGT 5 cut(s) 640, 934, 1087, 1471, 1647
HpyCH4IV ACGT 4 cut(s) 220, 1257, 1301, 1338
HpyF10VI GCNNNNNNNGC 9 cut(s) 295, 304, 323, 515, 790, 863, 970, 1064, 1496
HpyF3I CTNAG 1 cut(s) 594
HpySE526I ACGT 4 cut(s) 220, 1257, 1301, 1338
Hsp92I GRCGYC 1 cut(s) 1257
HspAI GCGC 2 cut(s) 102, 130
Kpn2I TCCGGA 1 cut(s) 435
Ksp22I TGATCA 2 cut(s) 660, 1392
Kzo9I GATC 7 cut(s) 161, 238, 419, 660, 914, 1392, 1603
LmnI GCTCC 4 cut(s) 57, 99, 187, 195
Lsp1109I GCAGC 5 cut(s) 521, 631, 802, 824, 1013
LweI GCATC 3 cut(s) 288, 707, 960
MabI ACCWGGT 1 cut(s) 1050
MaeI CTAG 3 cut(s) 183, 1322, 1376
MaeII ACGT 4 cut(s) 220, 1257, 1301, 1338
MaeIII GTNAC 5 cut(s) 120, 1339, 1348, 1465, 1527
MalI GATC 7 cut(s) 163, 240, 421, 662, 916, 1394, 1605
MboI GATC 7 cut(s) 161, 238, 419, 660, 914, 1392, 1603
MboII GAAGA 8 cut(s) 38, 92, 367, 881, 1082, 1288, 1395, 1607
MfeI CAATTG 2 cut(s) 819, 1577
MflI RGATCY 2 cut(s) 238, 1603
MhlI GDGCHC 5 cut(s) 39, 192, 1069, 1144, 1286
MluCI AATT 8 cut(s) 18, 94, 267, 389, 651, 688, 819, 1577
MlyI GAGTC 2 cut(s) 92, 144
MmeI TCCRAC 2 cut(s) 588, 1430
MroI TCCGGA 1 cut(s) 435
MseI TTAA 1 cut(s) 947
MslI CAYNNNNRTG 4 cut(s) 284, 312, 1304, 1648
MspA1I CMGCKG 2 cut(s) 410, 815
MspI CCGG 2 cut(s) 9, 436
MspR9I CCNGG 4 cut(s) 10, 611, 1052, 1231
MunI CAATTG 2 cut(s) 819, 1577
Mva1269I GAATGC 1 cut(s) 461
MvaI CCWGG 3 cut(s) 611, 1052, 1231
MvnI CGCG 1 cut(s) 130
MwoI GCNNNNNNNGC 9 cut(s) 295, 304, 323, 515, 790, 863, 970, 1064, 1496
NciI CCSGG 1 cut(s) 10
NcoI CCATGG 2 cut(s) 861, 1157
NdeII GATC 7 cut(s) 161, 238, 419, 660, 914, 1392, 1603
NlaIV GGNNCC 5 cut(s) 7, 615, 1189, 1547, 1556
NmeAIII GCCGAG 1 cut(s) 338
NmuCI GTSAC 5 cut(s) 120, 1339, 1348, 1465, 1527
OliI CACNNNNGTG 1 cut(s) 1648
PagI TCATGA 1 cut(s) 1209
PctI GAATGC 1 cut(s) 461
PfeI GAWTC 3 cut(s) 553, 1150, 1438
PflMI CCANNNNNTGG 3 cut(s) 413, 525, 1449
PfoI TCCNGGA 2 cut(s) 609, 1229
PkrI GCNGC 6 cut(s) 412, 511, 646, 814, 817, 1003
PleI GAGTC 2 cut(s) 91, 144
PpsI GAGTC 2 cut(s) 91, 144
Ppu21I YACGTR 1 cut(s) 1339
PshAI GACNNNNGTC 1 cut(s) 1346
Psp124BI GAGCTC 1 cut(s) 192
Psp1406I AACGTT 1 cut(s) 220
Psp6I CCWGG 3 cut(s) 609, 1050, 1229
PspEI GGTNACC 1 cut(s) 1527
PspGI CCWGG 3 cut(s) 609, 1050, 1229
PspN4I GGNNCC 5 cut(s) 7, 615, 1189, 1547, 1556
PspPI GGNCC 5 cut(s) 145, 320, 613, 791, 1041
PstNI CAGNNNCTG 1 cut(s) 413
PsuI RGATCY 2 cut(s) 238, 1603
PvuII CAGCTG 1 cut(s) 815
RsaI GTAC 1 cut(s) 1049
RsaNI GTAC 1 cut(s) 1048
RseI CAYNNNNRTG 4 cut(s) 284, 312, 1304, 1648
SacI GAGCTC 1 cut(s) 192
SaqAI TTAA 1 cut(s) 947
SatI GCNGC 6 cut(s) 411, 510, 645, 813, 816, 1002
Sau3AI GATC 7 cut(s) 161, 238, 419, 660, 914, 1392, 1603
Sau96I GGNCC 5 cut(s) 145, 320, 613, 791, 1041
SchI GAGTC 2 cut(s) 92, 144
ScrFI CCNGG 4 cut(s) 10, 611, 1052, 1231
SduI GDGCHC 5 cut(s) 39, 192, 1069, 1144, 1286
SexAI ACCWGGT 1 cut(s) 1050
SfaNI GCATC 3 cut(s) 288, 707, 960
SfcI CTRYAG 1 cut(s) 639
SinI GGWCC 3 cut(s) 145, 320, 613
SmiMI CAYNNNNRTG 4 cut(s) 284, 312, 1304, 1648
SmlI CTYRAG 2 cut(s) 488, 1070
SmoI CTYRAG 2 cut(s) 488, 1070
Sse9I AATT 8 cut(s) 18, 94, 267, 389, 651, 688, 819, 1577
SsiI CCGC 2 cut(s) 410, 702
SspI AATATT 2 cut(s) 723, 1590
SspMI CTAG 3 cut(s) 183, 1322, 1376
SstI GAGCTC 1 cut(s) 192
StyD4I CCNGG 4 cut(s) 8, 609, 1050, 1229
StyI CCWWGG 3 cut(s) 861, 1157, 1558
TaaI ACNGT 5 cut(s) 640, 934, 1087, 1471, 1647
TaiI ACGT 4 cut(s) 223, 1260, 1304, 1341
TaqI TCGA 3 cut(s) 33, 109, 399
TaqII GACCGA 2 cut(s) 162, 1358
TasI AATT 8 cut(s) 18, 94, 267, 389, 651, 688, 819, 1577
TauI GCSGC 1 cut(s) 413
TfiI GAWTC 3 cut(s) 553, 1150, 1438
Tru1I TTAA 1 cut(s) 947
Tru9I TTAA 1 cut(s) 947
TscAI CASTG 7 cut(s) 391, 598, 966, 1090, 1512, 1556, 1650
TseFI GTSAC 5 cut(s) 120, 1339, 1348, 1465, 1527
TseI GCWGC 5 cut(s) 509, 644, 812, 815, 1001
Tsp45I GTSAC 5 cut(s) 120, 1339, 1348, 1465, 1527
TspDTI ATGAA 4 cut(s) 186, 230, 454, 785
TspGWI ACGGA 1 cut(s) 852
TspRI CASTG 7 cut(s) 391, 598, 966, 1090, 1512, 1556, 1650
Van91I CCANNNNNTGG 3 cut(s) 413, 525, 1449
VpaK11BI GGWCC 3 cut(s) 145, 320, 613
XapI RAATTY 1 cut(s) 389
XcmI CCANNNNNNNNNTGG 2 cut(s) 1090, 1556
XspI CTAG 3 cut(s) 183, 1322, 1376
ZraI GACGTC 1 cut(s) 1258
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.