Prupe.4G118700_v2.0.a1

cationic amino acid transporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp04
OG0013891
Physical Location & Seq
Forward (+)
6425888 .. 6427656
1769 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.4G118700.1

Sequence Viewer

Length: 1677 bp
ATGGGTTCCATGGGAGTAGTGGGTGAAGAGGTTCAGCCAAGAAGTTACTGTTGGAGTACATATCGGACCGCCCTGTCACAAACAGGCTACAGGTTCAAGAACCGTCTCATAAGCAGATCAGATGATAACAATGAGATTGGGGAACTTCCAAAGCGAAGTGAAAATGACATGAAGCGTTGCCTCACATGGTGGGATCTCATCTGGTTTGGATTTGGTTCAGTAATTGGTGCAGGCATCTTTGTGCTTACTGGGCAAGAAATTCATGACCATGCTGGACCAGCTATTGTCTTGTCCTATGTTGCTTCCGGTGTCTCGGCAATGCTCTCTGTCTTCTCCAACACAGAATTTTCAGTGGAAGTCCCTGTGGCAGAATTAGGAGACTTTATAGCATTCACAACAGCAGGAAACATACTTCTTGAGGGCATTGTCGGAAGTGCAGCAATTGCTAGATCATGGACTTCTTACTTCACATCACTCTTGAATCGTCCCTCCAACTCATTACGCATCCATACAAATCTGACGGAGGGGTTCAATCTACTTGACCCAATAGCTGTTGCGGTTCTGACAATAGCTGCAACAATTGCAATGATCAGCACCAGGAAGACCTCTTACTTAAACTGGATAGCATCTGTAGTCAATAATGTTGTAATTCTGTTTGTGATAATTGCAGCATTTATCCATGCCAACCCATCAAATATGAAACCCTTTTTTCCGTTTGGGGCTAGAGGAGTCTTTCAAGCAGCTGCAATTGTTTACCTTGCTTACGGAGGATTTGACAATATCGCCACCATGGCTGAAGAAACCAAATATCCATCCAGGGACATACCGATAGGATTAATTGGATCAATGTCAATCATCACTGTCGTATATTGTTTAACGGCAGTTTCACTGCCTATGATGCAGAAATACACAGATATAGACCCAAATGTAGCCTACTCTGTAGCATTTCAAAGTGTGGGCATGACATGGGCCAAGTACCTAGTTGCCCTAGGTGCCCTGAAGGGAATGACCACTGTTCTTCTGGTCGGGACACTTGGACAGGCACGGTATATCACTCATATTGCACGAGCCCACATGATACCACCATGGTTTGCTCTTGTCCATCCAAAGACAGGAACCCCCATAAATGCTAGTCTTGATTGTCATTCCAAGTGGTTGTATTGCTTTGTTTTCGAGCTTGGATGTCTTGGCAGGTCTGTTATCATGAGCACACTCTTTATCTTCATGATGATGGCTGTCGCACTTCTAGTGAGGAGGTACTACGTGAGAGAAATCACCCCACAACATCATCTTTTGAAGCGTCATTTCTTGCTGATCATCATTGCTTCTTCCATGTGGACTTCGGCTTACTGGGGACTAAATCCCAATGGTTGGGTTGGTTATGTCGTGACAGTTCCTCTTTGGTTCTTTGGGACTTTGGCAATGGCAGTATTTTTACCCCAGCAGAGGTCACCAAAAGTTTGGGGGGTTTCCACTGGTTCCTTGGTTGCCATCCTTGTCAATTGCAACAAACATTTTTCTTATGGGCTCTCTGGATTTGGAATCTGCACCGTAGTAATGCTTATTTACTATGTCTTCTTTGGTCTGCATGCAACTTATGATATGGCCCATAAGCAAGACCAGCCAGAATCCCTGAAAGTTGATGACAGCAACATCAGAGAGAAGGCAGAGCCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AA_permease_2 AA_permease_C

Protein Analysis

559

Amino Acids

61.71

Weight (kDa)

8.63

Isoelectric Point (pI)

34.6

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013891)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G34960
fragaria_vesca FvH4_3g13060
malus_domestica MD05G1245200.v1.1 MD05G1245300.v1.1
prunus_persica Prupe.4G118600_v2.0.a1 Prupe.4G118700_v2.0.a1
pyrus_communis pycom10g19060
rosa_chinensis RchiOBHm_Chr5g0021251
rosa_laevigata RLG00000032599
rosa_multiflora Rmu_sc0004847.1_g000002
rosa_roxburghii Rroxscaffold_1G00057250
rosa_rugosa Rorug05G0066000
rosa_samantha Rh5AG154700 Rh5BG154200 Rh5CG167800 Rh5DG154800
rosa_wichuraiana Rw5G013790

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 73
Acc36I ACCTGC 1 cut(s) 1182
AccB1I GGYRCC 1 cut(s) 992
AccB7I CCANNNNNTGG 1 cut(s) 1371
AciI CCGC 2 cut(s) 69, 557
AclWI GGATC 2 cut(s) 201, 850
AcsI RAATTY 2 cut(s) 258, 344
AcuI CTGAAG 2 cut(s) 816, 1019
AdeI CACNNNGTG 1 cut(s) 189
AfaI GTAC 3 cut(s) 58, 977, 1259
AfiI CCNNNNNNNGG 3 cut(s) 1112, 1371, 1446
AgsI TTSAA 6 cut(s) 97, 481, 532, 737, 950, 1297
AjnI CCWGG 2 cut(s) 596, 815
AjuI GAANNNNNNNTTGG 2 cut(s) 34, 66
AluBI AGCT 5 cut(s) 281, 551, 572, 743, 1177
AluI AGCT 5 cut(s) 281, 551, 572, 743, 1177
Alw21I GWGCWC 1 cut(s) 1211
Alw26I GTCTC 3 cut(s) 110, 316, 372
AlwI GGATC 2 cut(s) 201, 850
AoxI GGCC 2 cut(s) 969, 1605
ApeKI GCWGC 5 cut(s) 437, 572, 668, 740, 743
ApoI RAATTY 2 cut(s) 258, 344
AseI ATTAAT 1 cut(s) 836
Asp700I GAANNNNTTC 1 cut(s) 30
AspA2I CCTAGG 1 cut(s) 988
AspS9I GGNCC 4 cut(s) 66, 275, 969, 1606
AsuHPI GGTGA 3 cut(s) 35, 1267, 1443
AvaII GGWCC 2 cut(s) 66, 275
AvrII CCTAGG 1 cut(s) 988
BaeGI GKGCMC 1 cut(s) 997
BanI GGYRCC 1 cut(s) 992
BanII GRGCYC 2 cut(s) 1072, 1530
BarI GAAGNNNNNNTAC 2 cut(s) 593, 625
BauI CACGAG 1 cut(s) 1065
BbsI GAAGAC 3 cut(s) 322, 608, 1567
Bbv12I GWGCWC 1 cut(s) 1211
BbvI GCAGC 5 cut(s) 449, 559, 680, 730, 752
BccI CCATC 5 cut(s) 697, 820, 1110, 1225, 1499
BceAI ACGGC 1 cut(s) 894
BciT130I CCWGG 2 cut(s) 598, 817
BclI TGATCA 2 cut(s) 588, 1314
BcoDI GTCTC 3 cut(s) 110, 316, 372
BfaI CTAG 6 cut(s) 447, 723, 980, 989, 1131, 1247
BfmI CTRYAG 3 cut(s) 88, 630, 939
BfuAI ACCTGC 1 cut(s) 1182
BglI GCCNNNNNGGC 1 cut(s) 791
BisI GCNGC 5 cut(s) 438, 573, 669, 741, 744
BlnI CCTAGG 1 cut(s) 988
BlsI GCNGC 5 cut(s) 439, 574, 670, 742, 745
Bme1390I CCNGG 2 cut(s) 598, 817
Bme18I GGWCC 2 cut(s) 66, 275
BmgT120I GGNCC 4 cut(s) 66, 275, 969, 1606
BmiI GGNNCC 4 cut(s) 7, 994, 1117, 1480
BmrFI CCNGG 2 cut(s) 598, 817
BmrI ACTGGG 2 cut(s) 258, 1360
BmsI GCATC 4 cut(s) 243, 513, 635, 888
BmuI ACTGGG 2 cut(s) 258, 1360
BpiI GAAGAC 3 cut(s) 322, 608, 1567
BpuEI CTTGAG 1 cut(s) 437
BsaAI YACGTR 1 cut(s) 1264
BsaJI CCNNGG 6 cut(s) 9, 789, 816, 988, 1085, 1482
BsaWI WCCGGW 1 cut(s) 305
Bsc4I CCNNNNNNNGG 3 cut(s) 1112, 1371, 1446
Bse1I ACTGG 4 cut(s) 253, 623, 1355, 1480
Bse3DI GCAATG 4 cut(s) 324, 591, 1320, 1428
BseBI CCWGG 2 cut(s) 598, 817
BseDI CCNNGG 6 cut(s) 9, 789, 816, 988, 1085, 1482
BseGI GGATG 5 cut(s) 504, 812, 1102, 1187, 1491
BseLI CCNNNNNNNGG 3 cut(s) 1112, 1371, 1446
BseMI GCAATG 4 cut(s) 324, 591, 1320, 1428
BseNI ACTGG 4 cut(s) 253, 623, 1355, 1480
BseRI GAGGAG 2 cut(s) 741, 1267
BseSI GKGCMC 1 cut(s) 997
BseXI GCAGC 5 cut(s) 449, 559, 680, 730, 752
BseYI CCCAGC 1 cut(s) 1440
BsgI GTGCAG 3 cut(s) 249, 456, 1531
BshFI GGCC 2 cut(s) 971, 1607
BshNI GGYRCC 1 cut(s) 992
BsiHKAI GWGCWC 1 cut(s) 1211
BsiSI CCGG 1 cut(s) 306
BslFI GGGAC 6 cut(s) 344, 471, 833, 1042, 1368, 1426
BslI CCNNNNNNNGG 3 cut(s) 1112, 1371, 1446
BsmAI GTCTC 3 cut(s) 110, 316, 372
BsmBI CGTCTC 1 cut(s) 110
BsmFI GGGAC 6 cut(s) 344, 471, 833, 1042, 1368, 1426
BsmI GAATGC 1 cut(s) 389
BsnI GGCC 2 cut(s) 971, 1607
Bsp1286I GDGCHC 4 cut(s) 997, 1072, 1211, 1530
Bsp143I GATC 6 cut(s) 116, 193, 449, 588, 842, 1314
Bsp19I CCATGG 3 cut(s) 9, 789, 1085
BspACI CCGC 2 cut(s) 69, 557
BspANI GGCC 2 cut(s) 971, 1607
BspHI TCATGA 3 cut(s) 262, 1203, 1224
BspLI GGNNCC 4 cut(s) 7, 994, 1117, 1480
BspMI ACCTGC 1 cut(s) 1182
BspPI GGATC 2 cut(s) 201, 850
BspT107I GGYRCC 1 cut(s) 992
BsrDI GCAATG 4 cut(s) 324, 591, 1320, 1428
BsrI ACTGG 4 cut(s) 253, 623, 1355, 1480
BssECI CCNNGG 6 cut(s) 9, 789, 816, 988, 1085, 1482
BssMI GATC 6 cut(s) 116, 193, 449, 588, 842, 1314
BssSI CACGAG 1 cut(s) 1065
BssT1I CCWWGG 5 cut(s) 9, 789, 988, 1085, 1482
Bst2BI CACGAG 1 cut(s) 1065
Bst2UI CCWGG 2 cut(s) 598, 817
Bst4CI ACNGT 7 cut(s) 50, 104, 862, 1015, 1047, 1393, 1552
Bst6I CTCTTC 1 cut(s) 21
BstAPI GCANNNNNTGC 2 cut(s) 443, 581
BstBAI YACGTR 1 cut(s) 1264
BstC8I GCNNGC 2 cut(s) 232, 1590
BstDSI CCRYGG 3 cut(s) 9, 789, 1085
BstEII GGTNACC 1 cut(s) 1449
BstF5I GGATG 5 cut(s) 504, 812, 1102, 1187, 1491
BstKTI GATC 6 cut(s) 119, 196, 452, 591, 845, 1317
BstMAI GTCTC 3 cut(s) 110, 316, 372
BstMBI GATC 6 cut(s) 116, 193, 449, 588, 842, 1314
BstMWI GCNNNNNNNGC 8 cut(s) 250, 278, 443, 581, 791, 898, 992, 1621
BstNI CCWGG 2 cut(s) 598, 817
BstNSI RCATGY 1 cut(s) 1592
BstPI GGTNACC 1 cut(s) 1449
BstSCI CCNGG 2 cut(s) 596, 815
BstSFI CTRYAG 3 cut(s) 88, 630, 939
BstSLI GKGCMC 1 cut(s) 997
BstV1I GCAGC 5 cut(s) 449, 559, 680, 730, 752
BstV2I GAAGAC 3 cut(s) 322, 608, 1567
BstX2I RGATCY 1 cut(s) 193
BstXI CCANNNNNNTGG 1 cut(s) 1461
BstYI RGATCY 1 cut(s) 193
BsuRI GGCC 2 cut(s) 971, 1607
BtgI CCRYGG 3 cut(s) 9, 789, 1085
BtsCI GGATG 5 cut(s) 504, 812, 1102, 1187, 1491
BtsI GCAGTG 1 cut(s) 887
BtsIMutI CAGTG 5 cut(s) 357, 858, 887, 1011, 1473
BveI ACCTGC 1 cut(s) 1182
Cac8I GCNNGC 2 cut(s) 232, 1590
CciI TCATGA 3 cut(s) 262, 1203, 1224
Cfr13I GGNCC 4 cut(s) 66, 275, 969, 1606
CpoI CGGWCCG 1 cut(s) 66
CseI GACGC 1 cut(s) 1289
Csp6I GTAC 3 cut(s) 57, 976, 1258
CspI CGGWCCG 1 cut(s) 66
CviQI GTAC 3 cut(s) 57, 976, 1258
DpnI GATC 6 cut(s) 118, 195, 451, 590, 844, 1316
DpnII GATC 6 cut(s) 116, 193, 449, 588, 842, 1314
DraIII CACNNNGTG 1 cut(s) 189
DrdI GACNNNNNNGTC 1 cut(s) 73
DseDI GACNNNNNNGTC 1 cut(s) 73
Eam1104I CTCTTC 1 cut(s) 21
EarI CTCTTC 1 cut(s) 21
Eco130I CCWWGG 5 cut(s) 9, 789, 988, 1085, 1482
Eco24I GRGCYC 2 cut(s) 1072, 1530
Eco47I GGWCC 2 cut(s) 66, 275
Eco57I CTGAAG 2 cut(s) 816, 1019
Eco91I GGTNACC 1 cut(s) 1449
EcoO65I GGTNACC 1 cut(s) 1449
EcoRII CCWGG 2 cut(s) 596, 815
EcoT14I CCWWGG 5 cut(s) 9, 789, 988, 1085, 1482
EcoT38I GRGCYC 2 cut(s) 1072, 1530
ErhI CCWWGG 5 cut(s) 9, 789, 988, 1085, 1482
Esp3I CGTCTC 1 cut(s) 110
FaqI GGGAC 6 cut(s) 344, 471, 833, 1042, 1368, 1426
FbaI TGATCA 2 cut(s) 588, 1314
Fnu4HI GCNGC 5 cut(s) 438, 573, 669, 741, 744
FokI GGATG 5 cut(s) 491, 799, 1089, 1194, 1478
FriOI GRGCYC 2 cut(s) 1072, 1530
Fsp4HI GCNGC 5 cut(s) 438, 573, 669, 741, 744
FspBI CTAG 6 cut(s) 447, 723, 980, 989, 1131, 1247
GluI GCNGC 5 cut(s) 438, 573, 669, 741, 744
GsaI CCCAGC 1 cut(s) 1444
HaeIII GGCC 2 cut(s) 971, 1607
HapII CCGG 1 cut(s) 306
HgaI GACGC 1 cut(s) 1289
HinfI GANTC 4 cut(s) 481, 729, 1542, 1628
HpaII CCGG 1 cut(s) 306
HphI GGTGA 3 cut(s) 35, 1267, 1443
Hpy166II GTNNAC 2 cut(s) 754, 1338
Hpy188I TCNGA 6 cut(s) 66, 121, 431, 519, 564, 1658
Hpy8I GTNNAC 2 cut(s) 754, 1338
HpyAV CCTTC 2 cut(s) 994, 1657
HpyCH4III ACNGT 7 cut(s) 50, 104, 862, 1015, 1047, 1393, 1552
HpyCH4IV ACGT 1 cut(s) 1263
HpyF10VI GCNNNNNNNGC 8 cut(s) 250, 278, 443, 581, 791, 898, 992, 1621
HpySE526I ACGT 1 cut(s) 1263
Ksp22I TGATCA 2 cut(s) 588, 1314
Kzo9I GATC 6 cut(s) 116, 193, 449, 588, 842, 1314
Lsp1109I GCAGC 5 cut(s) 449, 559, 680, 730, 752
LweI GCATC 4 cut(s) 243, 513, 635, 888
MaeI CTAG 6 cut(s) 447, 723, 980, 989, 1131, 1247
MaeII ACGT 1 cut(s) 1263
MaeIII GTNAC 4 cut(s) 44, 75, 1387, 1449
MalI GATC 6 cut(s) 118, 195, 451, 590, 844, 1316
MboI GATC 6 cut(s) 116, 193, 449, 588, 842, 1314
MboII GAAGA 8 cut(s) 38, 322, 613, 809, 1010, 1213, 1320, 1567
MfeI CAATTG 4 cut(s) 441, 579, 747, 1501
MflI RGATCY 1 cut(s) 193
MhlI GDGCHC 4 cut(s) 997, 1072, 1211, 1530
MlyI GAGTC 1 cut(s) 738
MmeI TCCRAC 4 cut(s) 32, 360, 409, 516
MroXI GAANNNNTTC 1 cut(s) 30
MseI TTAA 3 cut(s) 614, 836, 875
MslI CAYNNNNRTG 3 cut(s) 239, 267, 1229
MspA1I CMGCKG 1 cut(s) 743
MspI CCGG 1 cut(s) 306
MspR9I CCNGG 2 cut(s) 598, 817
MunI CAATTG 4 cut(s) 441, 579, 747, 1501
Mva1269I GAATGC 1 cut(s) 389
MvaI CCWGG 2 cut(s) 598, 817
MwoI GCNNNNNNNGC 8 cut(s) 250, 278, 443, 581, 791, 898, 992, 1621
NcoI CCATGG 3 cut(s) 9, 789, 1085
NdeII GATC 6 cut(s) 116, 193, 449, 588, 842, 1314
NlaIV GGNNCC 4 cut(s) 7, 994, 1117, 1480
NmeAIII GCCGAG 1 cut(s) 293
NmuCI GTSAC 3 cut(s) 75, 1387, 1449
NspI RCATGY 1 cut(s) 1592
PaeI GCATGC 1 cut(s) 1592
PagI TCATGA 3 cut(s) 262, 1203, 1224
PctI GAATGC 1 cut(s) 389
PdmI GAANNNNTTC 1 cut(s) 30
PfeI GAWTC 3 cut(s) 481, 1542, 1628
PflMI CCANNNNNTGG 1 cut(s) 1371
PkrI GCNGC 5 cut(s) 439, 574, 670, 742, 745
PleI GAGTC 1 cut(s) 737
PpsI GAGTC 1 cut(s) 737
Ppu21I YACGTR 1 cut(s) 1264
PshBI ATTAAT 1 cut(s) 836
Psp6I CCWGG 2 cut(s) 596, 815
PspEI GGTNACC 1 cut(s) 1449
PspFI CCCAGC 1 cut(s) 1440
PspGI CCWGG 2 cut(s) 596, 815
PspN4I GGNNCC 4 cut(s) 7, 994, 1117, 1480
PspPI GGNCC 4 cut(s) 66, 275, 969, 1606
PsuI RGATCY 1 cut(s) 193
PvuII CAGCTG 1 cut(s) 743
RsaI GTAC 3 cut(s) 58, 977, 1259
RsaNI GTAC 3 cut(s) 57, 976, 1258
RseI CAYNNNNRTG 3 cut(s) 239, 267, 1229
Rsr2I CGGWCCG 1 cut(s) 66
RsrII CGGWCCG 1 cut(s) 66
SaqAI TTAA 3 cut(s) 614, 836, 875
SatI GCNGC 5 cut(s) 438, 573, 669, 741, 744
Sau3AI GATC 6 cut(s) 116, 193, 449, 588, 842, 1314
Sau96I GGNCC 4 cut(s) 66, 275, 969, 1606
SchI GAGTC 1 cut(s) 738
ScrFI CCNGG 2 cut(s) 598, 817
SduI GDGCHC 4 cut(s) 997, 1072, 1211, 1530
SfaNI GCATC 4 cut(s) 243, 513, 635, 888
SfcI CTRYAG 3 cut(s) 88, 630, 939
SinI GGWCC 2 cut(s) 66, 275
SmiMI CAYNNNNRTG 3 cut(s) 239, 267, 1229
SmlI CTYRAG 1 cut(s) 416
SmoI CTYRAG 1 cut(s) 416
SphI GCATGC 1 cut(s) 1592
SsiI CCGC 2 cut(s) 69, 557
SspMI CTAG 6 cut(s) 447, 723, 980, 989, 1131, 1247
StyD4I CCNGG 2 cut(s) 596, 815
StyI CCWWGG 5 cut(s) 9, 789, 988, 1085, 1482
TaaI ACNGT 7 cut(s) 50, 104, 862, 1015, 1047, 1393, 1552
TaiI ACGT 1 cut(s) 1266
TaqI TCGA 1 cut(s) 1173
TatI WGTACW 1 cut(s) 56
TfiI GAWTC 3 cut(s) 481, 1542, 1628
Tru1I TTAA 3 cut(s) 614, 836, 875
Tru9I TTAA 3 cut(s) 614, 836, 875
TscAI CASTG 5 cut(s) 357, 865, 894, 1018, 1480
TseFI GTSAC 3 cut(s) 75, 1387, 1449
TseI GCWGC 5 cut(s) 437, 572, 668, 740, 743
Tsp45I GTSAC 3 cut(s) 75, 1387, 1449
TspDTI ATGAA 4 cut(s) 185, 251, 713, 1213
TspGWI ACGGA 3 cut(s) 536, 702, 780
TspRI CASTG 5 cut(s) 357, 865, 894, 1018, 1480
Van91I CCANNNNNTGG 1 cut(s) 1371
VpaK11BI GGWCC 2 cut(s) 66, 275
VspI ATTAAT 1 cut(s) 836
XapI RAATTY 2 cut(s) 258, 344
XceI RCATGY 1 cut(s) 1592
XcmI CCANNNNNNNNNTGG 3 cut(s) 16, 1018, 1480
XmaJI CCTAGG 1 cut(s) 988
XmnI GAANNNNTTC 1 cut(s) 30
XspI CTAG 6 cut(s) 447, 723, 980, 989, 1131, 1247
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.