RchiOBHm_Chr5g0021251

cationic amino acid transporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0013891
Physical Location & Seq
Forward (+)
15515525 .. 15517364
1840 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ30131

Sequence Viewer

Length: 1395 bp
ATGAGTTCCGAGGGAGAAATTGGTGAAGAAGTCCAGCCAAGAACCTACTGGAGATGGAGCAAACAAGATTTCCTGCCAGAAGAGTCCTTTCAAAATTGGAGCGCATATCGAACTGCCTTGTCACAGACGCGCCACAGGTTCAAGGACCGACTCATAAGCAGATCAAATGATGAAAATGAGACTAGGGAGCTCCCGAAACAAAGTGAGAATAACATGAAACGTTGCCTCACATGGTGGGATCTCATCTGGTTTGGTTTCGGTTCAGTAATTGGTGCAGGCATCTTTGTGCTTACTGGGCAAGAAACGCATAGACATGCTGGACCAGCTATTGTCTTGTCCTATGTTGCTTCAGGTATCTCGGCAATGCTCTCTGTCTTCTGCTACACTGAATTTTCAGTCGAAATACCAGCGGCTGGTGGATCTTTTGCTTACCTCCGGATTGAGTTAGGAGACTTTGTAGCATTCATAACAGCAGGAAATATACTTCTTGAGGGTGTTGTAGGAAGTGCAGCAGTTGCCAGAGCCTGGACTTCTTACTTCACATCGCTCTTGAATCGTCCCTCCAACTCATTACGCTTCCACACAAATCTCACTGGGGGCTACAATCTCCTGGACCCAATAGCTGTTGTTGTTCTGGCTGTAGCTGCTACAATTACAATGATCAGCACAAGGAAAACCTCTTACTTGAATTGGATAGCATCCGCAGTCAATAATGTTGTAATATTGTTTGTATTGATTGCAGGGTTTTCCCAAGCTAAACTCTCAAATATGAAACCTTTTATGCCTTTTGGGCCTAAAGGGGTCTTTCAAGCAGCTGCAATTGTTTACTTTGCTTACGGAGGATTTGACAACATTGCCACCATGGCCGAAGAAACCAAAAATCCATCCAGAGACATACCGATTGGATTGCTTGGATCAATGTCAATCATAACTGTTGTATATTGTTTAATGGCACTTTCACTGCCTATGATGCAGAAATACACAGACATAGACCCAAATGCAGCTTACTCTACCGCATTTCAAAGTGTGGGCATGACATGGGCCAAGTACCTGGTAGCTCTTGGTGCTCTCAAGGGAATGACCACTGTTCTTCTGGTGGGAACACTTGGACAGGCTCGGTATATCACTCACATAGCACGAGCCCACATGATTCCACCATGGTTTGCTCTTGTCCATCCAAAGACAGGAACCCCCATAAATGCTACACTCTTGATAGTTGTTTCAAGTTCCTGCATTGCTTTCTTTTCAAGCTTGGACGTCTTGACAAGCCTGTTATCGGTGAGCACACTCTTTATCTTCACGTTGATGGCTGTTGCACTTCTAGTGAGGAGATACTACGTGACAGATGTCACCCCAAAACCACATCTCTTGAAGCTAGTTCTTACTGGGGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AA_permease_2 AA_permease_C

Protein Analysis

464

Amino Acids

51.02

Weight (kDa)

8.88

Isoelectric Point (pI)

32.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AA_permease_2 PF13520 76 - 445 5.7e-47 Amino acid permease
AA_permease PF00324 81 - 441 2e-29 Amino acid permease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013891)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G34960
fragaria_vesca FvH4_3g13060
malus_domestica MD05G1245200.v1.1 MD05G1245300.v1.1
prunus_persica Prupe.4G118600_v2.0.a1 Prupe.4G118700_v2.0.a1
pyrus_communis pycom10g19060
rosa_chinensis RchiOBHm_Chr5g0021251
rosa_laevigata RLG00000032599
rosa_multiflora Rmu_sc0004847.1_g000002
rosa_roxburghii Rroxscaffold_1G00057250
rosa_rugosa Rorug05G0066000
rosa_samantha Rh5AG154700 Rh5BG154200 Rh5CG167800 Rh5DG154800
rosa_wichuraiana Rw5G013790

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1260
AccB7I CCANNNNNTGG 2 cut(s) 413, 525
AccII CGCG 1 cut(s) 130
AccIII TCCGGA 1 cut(s) 435
AciI CCGC 3 cut(s) 410, 702, 1014
AclI AACGTT 1 cut(s) 220
AclWI GGATC 3 cut(s) 246, 427, 922
AcoI YGGCCR 1 cut(s) 864
AcsI RAATTY 1 cut(s) 389
AcuI CTGAAG 1 cut(s) 333
AcyI GRCGYC 1 cut(s) 1257
AdeI CACNNNGTG 1 cut(s) 234
AfaI GTAC 1 cut(s) 1049
AfiI CCNNNNNNNGG 4 cut(s) 413, 525, 1184, 1276
AgsI TTSAA 9 cut(s) 92, 142, 553, 688, 809, 1022, 1224, 1248, 1372
AjnI CCWGG 3 cut(s) 524, 609, 1050
Alw21I GWGCWC 3 cut(s) 192, 1069, 1286
Alw26I GTCTC 3 cut(s) 173, 444, 885
AlwI GGATC 3 cut(s) 246, 427, 922
AlwNI CAGNNNCTG 2 cut(s) 413, 525
Aor13HI TCCGGA 1 cut(s) 435
AoxI GGCC 3 cut(s) 791, 864, 1041
ApeKI GCWGC 5 cut(s) 509, 644, 812, 815, 1001
ApoI RAATTY 1 cut(s) 389
AspLEI GCGC 2 cut(s) 104, 132
AspS9I GGNCC 5 cut(s) 145, 320, 613, 791, 1041
AsuHPI GGTGA 3 cut(s) 35, 1291, 1342
AvaII GGWCC 3 cut(s) 145, 320, 613
BanII GRGCYC 2 cut(s) 192, 1144
BauI CACGAG 1 cut(s) 1137
BbsI GAAGAC 1 cut(s) 367
Bbv12I GWGCWC 3 cut(s) 192, 1069, 1286
BbvI GCAGC 5 cut(s) 521, 631, 802, 824, 1013
BccI CCATC 4 cut(s) 48, 892, 1182, 1300
BcgI CGANNNNNNTGC 2 cut(s) 889, 923
BciT130I CCWGG 3 cut(s) 526, 611, 1052
BclI TGATCA 1 cut(s) 660
BcoDI GTCTC 3 cut(s) 173, 444, 885
BfaI CTAG 3 cut(s) 183, 1322, 1376
BfmI CTRYAG 1 cut(s) 639
BglI GCCNNNNNGGC 2 cut(s) 790, 863
BisI GCNGC 6 cut(s) 411, 510, 645, 813, 816, 1002
BlsI GCNGC 6 cut(s) 412, 511, 646, 814, 817, 1003
Bme1390I CCNGG 3 cut(s) 526, 611, 1052
Bme18I GGWCC 3 cut(s) 145, 320, 613
BmgT120I GGNCC 5 cut(s) 145, 320, 613, 791, 1041
BmiI GGNNCC 2 cut(s) 615, 1189
BmrFI CCNGG 3 cut(s) 526, 611, 1052
BmrI ACTGGG 3 cut(s) 303, 603, 1395
BmsI GCATC 3 cut(s) 288, 707, 960
BmuI ACTGGG 3 cut(s) 303, 603, 1395
BoxI GACNNNNGTC 1 cut(s) 1346
BpiI GAAGAC 1 cut(s) 367
BpmI CTGGAG 1 cut(s) 70
BpuEI CTTGAG 2 cut(s) 509, 1055
BsaAI YACGTR 1 cut(s) 1339
BsaHI GRCGYC 1 cut(s) 1257
BsaJI CCNNGG 3 cut(s) 9, 861, 1157
BsaWI WCCGGW 1 cut(s) 435
BsaXI ACNNNNNCTCC 2 cut(s) 441, 471
Bsc4I CCNNNNNNNGG 4 cut(s) 413, 525, 1184, 1276
Bse1I ACTGG 4 cut(s) 53, 298, 598, 1390
Bse3DI GCAATG 3 cut(s) 369, 852, 1233
BseAI TCCGGA 1 cut(s) 435
BseBI CCWGG 3 cut(s) 526, 611, 1052
BseDI CCNNGG 3 cut(s) 9, 861, 1157
BseGI GGATG 3 cut(s) 698, 884, 1174
BseLI CCNNNNNNNGG 4 cut(s) 413, 525, 1184, 1276
BseMI GCAATG 3 cut(s) 369, 852, 1233
BseNI ACTGG 4 cut(s) 53, 298, 598, 1390
BseRI GAGGAG 1 cut(s) 1342
BseXI GCAGC 5 cut(s) 521, 631, 802, 824, 1013
BsgI GTGCAG 2 cut(s) 294, 528
Bsh1236I CGCG 1 cut(s) 130
BshFI GGCC 3 cut(s) 793, 866, 1043
BsiHKAI GWGCWC 3 cut(s) 192, 1069, 1286
BsiSI CCGG 1 cut(s) 436
BslFI GGGAC 1 cut(s) 543
BslI CCNNNNNNNGG 4 cut(s) 413, 525, 1184, 1276
BsmAI GTCTC 3 cut(s) 173, 444, 885
BsmFI GGGAC 1 cut(s) 543
BsmI GAATGC 1 cut(s) 461
BsnI GGCC 3 cut(s) 793, 866, 1043
Bsp1286I GDGCHC 4 cut(s) 192, 1069, 1144, 1286
Bsp13I TCCGGA 1 cut(s) 435
Bsp143I GATC 5 cut(s) 161, 238, 419, 660, 914
Bsp19I CCATGG 2 cut(s) 861, 1157
BspACI CCGC 3 cut(s) 410, 702, 1014
BspANI GGCC 3 cut(s) 793, 866, 1043
BspEI TCCGGA 1 cut(s) 435
BspFNI CGCG 1 cut(s) 130
BspLI GGNNCC 2 cut(s) 615, 1189
BspPI GGATC 3 cut(s) 246, 427, 922
BsrDI GCAATG 3 cut(s) 369, 852, 1233
BsrI ACTGG 4 cut(s) 53, 298, 598, 1390
BssECI CCNNGG 3 cut(s) 9, 861, 1157
BssMI GATC 5 cut(s) 161, 238, 419, 660, 914
BssNI GRCGYC 1 cut(s) 1257
BssSI CACGAG 1 cut(s) 1137
BssT1I CCWWGG 2 cut(s) 861, 1157
Bst2BI CACGAG 1 cut(s) 1137
Bst2UI CCWGG 3 cut(s) 526, 611, 1052
Bst4CI ACNGT 2 cut(s) 934, 1087
Bst6I CTCTTC 1 cut(s) 75
BstACI GRCGYC 1 cut(s) 1257
BstAPI GCANNNNNTGC 1 cut(s) 515
BstBAI YACGTR 1 cut(s) 1339
BstC8I GCNNGC 1 cut(s) 277
BstDSI CCRYGG 2 cut(s) 861, 1157
BstF5I GGATG 3 cut(s) 698, 884, 1174
BstFNI CGCG 1 cut(s) 130
BstHHI GCGC 2 cut(s) 104, 132
BstKTI GATC 5 cut(s) 164, 241, 422, 663, 917
BstMAI GTCTC 3 cut(s) 173, 444, 885
BstMBI GATC 5 cut(s) 161, 238, 419, 660, 914
BstMWI GCNNNNNNNGC 9 cut(s) 295, 304, 323, 515, 644, 790, 863, 970, 1064
BstNI CCWGG 3 cut(s) 526, 611, 1052
BstNSI RCATGY 1 cut(s) 317
BstPAI GACNNNNGTC 1 cut(s) 1346
BstSCI CCNGG 3 cut(s) 524, 609, 1050
BstSFI CTRYAG 1 cut(s) 639
BstUI CGCG 1 cut(s) 130
BstV1I GCAGC 5 cut(s) 521, 631, 802, 824, 1013
BstV2I GAAGAC 1 cut(s) 367
BstX2I RGATCY 2 cut(s) 238, 419
BstXI CCANNNNNNTGG 1 cut(s) 1051
BstYI RGATCY 2 cut(s) 238, 419
BsuRI GGCC 3 cut(s) 793, 866, 1043
BtgI CCRYGG 2 cut(s) 861, 1157
BtgZI GCGATG 1 cut(s) 528
BtsCI GGATG 3 cut(s) 698, 884, 1174
BtsI GCAGTG 1 cut(s) 959
BtsIMutI CAGTG 4 cut(s) 384, 591, 959, 1083
Cac8I GCNNGC 1 cut(s) 277
CaiI CAGNNNCTG 2 cut(s) 413, 525
CfoI GCGC 2 cut(s) 104, 132
Cfr13I GGNCC 5 cut(s) 145, 320, 613, 791, 1041
CseI GACGC 1 cut(s) 136
CsiI ACCWGGT 1 cut(s) 1050
Csp6I GTAC 1 cut(s) 1048
CspCI CAANNNNNGTGG 2 cut(s) 1350, 1385
CviAII CATG 8 cut(s) 214, 231, 314, 862, 1033, 1038, 1147, 1158
CviQI GTAC 1 cut(s) 1048
DpnI GATC 5 cut(s) 163, 240, 421, 662, 916
DpnII GATC 5 cut(s) 161, 238, 419, 660, 914
DraIII CACNNNGTG 1 cut(s) 234
EaeI YGGCCR 1 cut(s) 864
Eam1104I CTCTTC 1 cut(s) 75
EarI CTCTTC 1 cut(s) 75
Ecl136II GAGCTC 1 cut(s) 190
Eco130I CCWWGG 2 cut(s) 861, 1157
Eco24I GRGCYC 2 cut(s) 192, 1144
Eco47I GGWCC 3 cut(s) 145, 320, 613
Eco53kI GAGCTC 1 cut(s) 190
Eco57I CTGAAG 1 cut(s) 333
EcoICRI GAGCTC 1 cut(s) 190
EcoRII CCWGG 3 cut(s) 524, 609, 1050
EcoT14I CCWWGG 2 cut(s) 861, 1157
EcoT38I GRGCYC 2 cut(s) 192, 1144
ErhI CCWWGG 2 cut(s) 861, 1157
FaeI CATG 8 cut(s) 217, 234, 317, 865, 1036, 1041, 1150, 1161
FaqI GGGAC 1 cut(s) 543
FatI CATG 8 cut(s) 213, 230, 313, 861, 1032, 1037, 1146, 1157
FbaI TGATCA 1 cut(s) 660
Fnu4HI GCNGC 6 cut(s) 411, 510, 645, 813, 816, 1002
FokI GGATG 3 cut(s) 685, 871, 1161
FriOI GRGCYC 2 cut(s) 192, 1144
Fsp4HI GCNGC 6 cut(s) 411, 510, 645, 813, 816, 1002
FspBI CTAG 3 cut(s) 183, 1322, 1376
GlaI GCGC 2 cut(s) 103, 131
GluI GCNGC 6 cut(s) 411, 510, 645, 813, 816, 1002
GsuI CTGGAG 1 cut(s) 70
HaeIII GGCC 3 cut(s) 793, 866, 1043
HapII CCGG 1 cut(s) 436
HgaI GACGC 1 cut(s) 136
HhaI GCGC 2 cut(s) 104, 132
Hin1I GRCGYC 1 cut(s) 1257
Hin1II CATG 8 cut(s) 217, 234, 317, 865, 1036, 1041, 1150, 1161
Hin6I GCGC 2 cut(s) 102, 130
HinP1I GCGC 2 cut(s) 102, 130
HindIII AAGCTT 1 cut(s) 1249
HinfI GANTC 4 cut(s) 83, 150, 553, 1150
HpaII CCGG 1 cut(s) 436
HphI GGTGA 3 cut(s) 35, 1291, 1342
Hpy166II GTNNAC 1 cut(s) 826
Hpy188I TCNGA 1 cut(s) 10
Hpy188III TCNNGA 8 cut(s) 193, 436, 488, 550, 888, 1210, 1261, 1369
Hpy8I GTNNAC 1 cut(s) 826
HpyCH4III ACNGT 2 cut(s) 934, 1087
HpyCH4IV ACGT 4 cut(s) 220, 1257, 1301, 1338
HpyCH4V TGCA 8 cut(s) 275, 509, 740, 818, 973, 1001, 1233, 1316
HpyF10VI GCNNNNNNNGC 9 cut(s) 295, 304, 323, 515, 644, 790, 863, 970, 1064
HpySE526I ACGT 4 cut(s) 220, 1257, 1301, 1338
Hsp92I GRCGYC 1 cut(s) 1257
Hsp92II CATG 8 cut(s) 217, 234, 317, 865, 1036, 1041, 1150, 1161
HspAI GCGC 2 cut(s) 102, 130
Kpn2I TCCGGA 1 cut(s) 435
Ksp22I TGATCA 1 cut(s) 660
Kzo9I GATC 5 cut(s) 161, 238, 419, 660, 914
LmnI GCTCC 4 cut(s) 57, 99, 187, 195
Lsp1109I GCAGC 5 cut(s) 521, 631, 802, 824, 1013
LweI GCATC 3 cut(s) 288, 707, 960
MabI ACCWGGT 1 cut(s) 1050
MaeI CTAG 3 cut(s) 183, 1322, 1376
MaeII ACGT 4 cut(s) 220, 1257, 1301, 1338
MaeIII GTNAC 3 cut(s) 120, 1339, 1348
MalI GATC 5 cut(s) 163, 240, 421, 662, 916
MboI GATC 5 cut(s) 161, 238, 419, 660, 914
MboII GAAGA 6 cut(s) 38, 92, 367, 881, 1082, 1288
MfeI CAATTG 1 cut(s) 819
MflI RGATCY 2 cut(s) 238, 419
MhlI GDGCHC 4 cut(s) 192, 1069, 1144, 1286
MluCI AATT 7 cut(s) 18, 94, 267, 389, 651, 688, 819
MlyI GAGTC 2 cut(s) 92, 144
MmeI TCCRAC 1 cut(s) 588
MnlI CCTC 8 cut(s) 4, 236, 443, 484, 571, 688, 833, 1320
MroI TCCGGA 1 cut(s) 435
MseI TTAA 1 cut(s) 947
MslI CAYNNNNRTG 3 cut(s) 284, 312, 1304
MspA1I CMGCKG 2 cut(s) 410, 815
MspI CCGG 1 cut(s) 436
MspR9I CCNGG 3 cut(s) 526, 611, 1052
MunI CAATTG 1 cut(s) 819
Mva1269I GAATGC 1 cut(s) 461
MvaI CCWGG 3 cut(s) 526, 611, 1052
MvnI CGCG 1 cut(s) 130
MwoI GCNNNNNNNGC 9 cut(s) 295, 304, 323, 515, 644, 790, 863, 970, 1064
NcoI CCATGG 2 cut(s) 861, 1157
NdeII GATC 5 cut(s) 161, 238, 419, 660, 914
NlaIII CATG 8 cut(s) 217, 234, 317, 865, 1036, 1041, 1150, 1161
NlaIV GGNNCC 2 cut(s) 615, 1189
NmeAIII GCCGAG 1 cut(s) 338
NmuCI GTSAC 3 cut(s) 120, 1339, 1348
NspI RCATGY 1 cut(s) 317
PctI GAATGC 1 cut(s) 461
PfeI GAWTC 2 cut(s) 553, 1150
PflMI CCANNNNNTGG 2 cut(s) 413, 525
PfoI TCCNGGA 1 cut(s) 609
PkrI GCNGC 6 cut(s) 412, 511, 646, 814, 817, 1003
PleI GAGTC 2 cut(s) 91, 144
PpsI GAGTC 2 cut(s) 91, 144
Ppu21I YACGTR 1 cut(s) 1339
PshAI GACNNNNGTC 1 cut(s) 1346
Psp124BI GAGCTC 1 cut(s) 192
Psp1406I AACGTT 1 cut(s) 220
Psp6I CCWGG 3 cut(s) 524, 609, 1050
PspGI CCWGG 3 cut(s) 524, 609, 1050
PspN4I GGNNCC 2 cut(s) 615, 1189
PspPI GGNCC 5 cut(s) 145, 320, 613, 791, 1041
PstNI CAGNNNCTG 2 cut(s) 413, 525
PsuI RGATCY 2 cut(s) 238, 419
PvuII CAGCTG 1 cut(s) 815
RsaI GTAC 1 cut(s) 1049
RsaNI GTAC 1 cut(s) 1048
RseI CAYNNNNRTG 3 cut(s) 284, 312, 1304
SacI GAGCTC 1 cut(s) 192
SaqAI TTAA 1 cut(s) 947
SatI GCNGC 6 cut(s) 411, 510, 645, 813, 816, 1002
Sau3AI GATC 5 cut(s) 161, 238, 419, 660, 914
Sau96I GGNCC 5 cut(s) 145, 320, 613, 791, 1041
SchI GAGTC 2 cut(s) 92, 144
ScrFI CCNGG 3 cut(s) 526, 611, 1052
SduI GDGCHC 4 cut(s) 192, 1069, 1144, 1286
SexAI ACCWGGT 1 cut(s) 1050
SfaNI GCATC 3 cut(s) 288, 707, 960
SfcI CTRYAG 1 cut(s) 639
SinI GGWCC 3 cut(s) 145, 320, 613
SmiMI CAYNNNNRTG 3 cut(s) 284, 312, 1304
SmlI CTYRAG 2 cut(s) 488, 1070
SmoI CTYRAG 2 cut(s) 488, 1070
Sse9I AATT 7 cut(s) 18, 94, 267, 389, 651, 688, 819
SsiI CCGC 3 cut(s) 410, 702, 1014
SspI AATATT 1 cut(s) 723
SspMI CTAG 3 cut(s) 183, 1322, 1376
SstI GAGCTC 1 cut(s) 192
StyD4I CCNGG 3 cut(s) 524, 609, 1050
StyI CCWWGG 2 cut(s) 861, 1157
TaaI ACNGT 2 cut(s) 934, 1087
TaiI ACGT 4 cut(s) 223, 1260, 1304, 1341
TaqI TCGA 2 cut(s) 109, 399
TaqII GACCGA 1 cut(s) 162
TasI AATT 7 cut(s) 18, 94, 267, 389, 651, 688, 819
TauI GCSGC 1 cut(s) 413
TfiI GAWTC 2 cut(s) 553, 1150
Tru1I TTAA 1 cut(s) 947
Tru9I TTAA 1 cut(s) 947
TscAI CASTG 4 cut(s) 391, 598, 966, 1090
TseFI GTSAC 3 cut(s) 120, 1339, 1348
TseI GCWGC 5 cut(s) 509, 644, 812, 815, 1001
Tsp45I GTSAC 3 cut(s) 120, 1339, 1348
TspDTI ATGAA 4 cut(s) 186, 230, 454, 785
TspGWI ACGGA 1 cut(s) 852
TspRI CASTG 4 cut(s) 391, 598, 966, 1090
Van91I CCANNNNNTGG 2 cut(s) 413, 525
VpaK11BI GGWCC 3 cut(s) 145, 320, 613
XapI RAATTY 1 cut(s) 389
XceI RCATGY 1 cut(s) 317
XcmI CCANNNNNNNNNTGG 2 cut(s) 45, 1090
XspI CTAG 3 cut(s) 183, 1322, 1376
ZraI GACGTC 1 cut(s) 1258
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.