Rw5G013790

cationic amino acid transporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0013891
Physical Location & Seq
Forward (+)
16297495 .. 16299285
1791 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G013790.1

Sequence Viewer

Length: 1791 bp
ATGGGTTCCGAGGGAGAAATTGGTGAAGAAGTCCAGCCAAGAACCTACTGGAGATGGAGCAAACAAGATTTCCTGCCAGAAGAGTCCTTTCAAAGTTGGAGCGCATATCTAACTGCCTTGTCACAGACGCGCCACAGGTTCAAGGACCGACTCATAAGCAGATCAAATGATGAAACTGAGACTGGGGAGCTTCGGAAACAAAGTGAGAATGACATGAAACGTTGCCTCACCTGGTGGGATCTCATCTGGTTTGGTTTCGGTTCAGTCATTGGTGCAGGCATCTTTGTGCTTACTGGGCAAGAAACGCATGACCATGCTGGACCAGCTATTGTCTTGTCCTATGTTGCTTCAGGTATCTCAGCAATGCTCTCTGTCTTCTGCTACACTGAATTTTCAGTCGAAATACCAGTAGCTGGTGGATCTTTCGCTTACCTGCGGATTGAGTTGGGAGACTTTGTAGCATTCATAACAGCAGGAAACATACTTCTTGAGAGTGTTGTAGGAAGTGCAGCAGTTGCCAGATCATGGACTTCTTACTTCACATCGCTCTTGAATCGTCCCCCCAACTCATTACGCTTCCACACAAATCTCACTGAGGGCTACAATCTCCTGGACCCAATAGCTGTTGTTGTTCTGGCTGTAGCTGCTACAATTACAATGATCAGCACAAGGAAAACCTCTTACTTGAATTGGATAGCATCCGCAGTCAATAATGTTGTAATATTGTTTGTATTGATTGCAGGGTTTTCCCATGCTAATCTCTCAAATATGAAACCTTTTATGCCGTTTGGGCCTAAAGGGGTCTTTCAAGCAGCTGCAATTGTTTACTTTGCCTATGGAGGATTTGACAACATTGCCACCATGGCTGAAGAAACCAAAAATCCATCCAGAGACATACCGATTGGATTGCTTGGATCAATGTCAATCATAACTGTCGTATATTGTTTAATGGCACTTTCACTGCCTATGATGCAGAAATACACAGATATAGACACAAATGCAGCTTACTCTATTGCATTTCAAAGTGTGGGCATGACATGGGCCAAGTACCTGGTAGCTCTTGGTGCTCTCAAGGGAATGACCACTGTTCTTCTGGTGGGAACACTTGGACAGGCTCGGTACATCACTCACATAGCACGAGCCCACATGATTCCACCATGGTTTGCTCTTGTCCATCCAAAAACAGGAACCCCCATAAACGCTACACTCTTGATAGCTGTTTTGAGTTCCTGCATTGCTTTCTTTTCAAGCTTGAACGTCTTGGCAAGCCTGTTATCGGTGAGCACACTCTTTATCTTCATGATGATGGCTGTTGCACTTCTAGTGAGGAGATACTATGTGAGAGATGTCACCCCAAAACCACATCTCTTGAAGCTAGTTGTTTACTTGGTGATTATTATTTCTTCCTCAATAGGGACTTCAGCTTACTGGGGATTGAATCCCGATGGTTGGATTGGTTATGTTGTGACAGTTCCTGTTTGGTTCTTTGCAACTTTGGCAATGTCAGTGTTTCTACCTCAACAGAGGTCACCAAAAGTCTGGGGGGTTCCACTGGTTCCTTGGTTGCCATCCTTGTCAATAGCCACAAATATTTTTCTTATGGGATCTTTGGGTTCTTCTGCTTTTGAAAGATTTGGAATATGCACTGTTGTGATGCTTATATACTATGCTTTCTTTGGCCTTCATGCAACCTATGATATGGCCCATAAGAAAGGTATGCCAGAACTGCCAGAATCACCAGAACCCCTGAAAGTTATGGATAGCAACACCAGAGAGAAGGCAGAACCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AA_permease_2 AA_permease_C

Protein Analysis

596

Amino Acids

65.76

Weight (kDa)

7.67

Isoelectric Point (pI)

38.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AA_permease_2 PF13520 76 - 478 1.4e-48 Amino acid permease
AA_permease PF00324 81 - 471 2.4e-29 Amino acid permease
AA_permease_C PF13906 514 - 563 1.1e-17 C-terminus of AA_permease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013891)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G34960
fragaria_vesca FvH4_3g13060
malus_domestica MD05G1245200.v1.1 MD05G1245300.v1.1
prunus_persica Prupe.4G118600_v2.0.a1 Prupe.4G118700_v2.0.a1
pyrus_communis pycom10g19060
rosa_chinensis RchiOBHm_Chr5g0021251
rosa_laevigata RLG00000032599
rosa_multiflora Rmu_sc0004847.1_g000002
rosa_roxburghii Rroxscaffold_1G00057250
rosa_rugosa Rorug05G0066000
rosa_samantha Rh5AG154700 Rh5BG154200 Rh5CG167800 Rh5DG154800
rosa_wichuraiana Rw5G013790

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 441
AccB7I CCANNNNNTGG 2 cut(s) 413, 525
AccII CGCG 1 cut(s) 130
AciI CCGC 2 cut(s) 436, 702
AclI AACGTT 1 cut(s) 220
AclWI GGATC 4 cut(s) 246, 427, 922, 1612
AcsI RAATTY 1 cut(s) 389
AcuI CTGAAG 3 cut(s) 333, 888, 1404
AdeI CACNNNGTG 1 cut(s) 234
AfaI GTAC 2 cut(s) 1049, 1121
AfiI CCNNNNNNNGG 6 cut(s) 413, 525, 1184, 1276, 1413, 1449
AjnI CCWGG 3 cut(s) 230, 609, 1050
AleI CACNNNNGTG 1 cut(s) 1649
Alw21I GWGCWC 2 cut(s) 1069, 1286
Alw26I GTCTC 3 cut(s) 173, 444, 885
AlwI GGATC 4 cut(s) 246, 427, 922, 1612
AlwNI CAGNNNCTG 2 cut(s) 413, 1475
AoxI GGCC 4 cut(s) 791, 1041, 1678, 1701
ApeKI GCWGC 5 cut(s) 509, 644, 812, 815, 1001
ApoI RAATTY 1 cut(s) 389
AspLEI GCGC 2 cut(s) 104, 132
AspS9I GGNCC 6 cut(s) 145, 320, 613, 791, 1041, 1702
AsuHPI GGTGA 7 cut(s) 35, 220, 1291, 1342, 1402, 1521, 1728
AvaII GGWCC 3 cut(s) 145, 320, 613
BanII GRGCYC 1 cut(s) 1144
BauI CACGAG 1 cut(s) 1137
BbsI GAAGAC 1 cut(s) 367
Bbv12I GWGCWC 2 cut(s) 1069, 1286
BbvI GCAGC 5 cut(s) 521, 631, 802, 824, 1013
BccI CCATC 6 cut(s) 48, 892, 1182, 1300, 1439, 1576
BceAI ACGGC 1 cut(s) 769
BcgI CGANNNNNNTGC 2 cut(s) 889, 923
BciT130I CCWGG 3 cut(s) 232, 611, 1052
BclI TGATCA 1 cut(s) 660
BcoDI GTCTC 3 cut(s) 173, 444, 885
BfaI CTAG 2 cut(s) 1322, 1376
BfmI CTRYAG 1 cut(s) 639
BfuAI ACCTGC 1 cut(s) 441
BglI GCCNNNNNGGC 2 cut(s) 790, 863
BisI GCNGC 5 cut(s) 510, 645, 813, 816, 1002
BlsI GCNGC 5 cut(s) 511, 646, 814, 817, 1003
Bme1390I CCNGG 3 cut(s) 232, 611, 1052
Bme18I GGWCC 3 cut(s) 145, 320, 613
BmgT120I GGNCC 6 cut(s) 145, 320, 613, 791, 1041, 1702
BmiI GGNNCC 5 cut(s) 7, 615, 1189, 1548, 1557
BmrFI CCNGG 3 cut(s) 232, 611, 1052
BmrI ACTGGG 3 cut(s) 192, 303, 1438
BmsI GCATC 4 cut(s) 288, 707, 960, 1644
BmuI ACTGGG 3 cut(s) 192, 303, 1438
BpiI GAAGAC 1 cut(s) 367
BpmI CTGGAG 1 cut(s) 70
BpuEI CTTGAG 2 cut(s) 509, 1055
BsaJI CCNNGG 4 cut(s) 9, 861, 1157, 1559
BsaXI ACNNNNNCTCC 2 cut(s) 441, 471
Bsc4I CCNNNNNNNGG 6 cut(s) 413, 525, 1184, 1276, 1413, 1449
Bse1I ACTGG 6 cut(s) 53, 187, 298, 407, 1433, 1557
Bse3DI GCAATG 4 cut(s) 369, 852, 1233, 1506
BseBI CCWGG 3 cut(s) 232, 611, 1052
BseDI CCNNGG 4 cut(s) 9, 861, 1157, 1559
BseGI GGATG 4 cut(s) 698, 884, 1174, 1568
BseLI CCNNNNNNNGG 6 cut(s) 413, 525, 1184, 1276, 1413, 1449
BseMI GCAATG 4 cut(s) 369, 852, 1233, 1506
BseMII CTCAG 3 cut(s) 168, 372, 585
BseNI ACTGG 6 cut(s) 53, 187, 298, 407, 1433, 1557
BseRI GAGGAG 1 cut(s) 1342
BseXI GCAGC 5 cut(s) 521, 631, 802, 824, 1013
BsgI GTGCAG 2 cut(s) 294, 528
Bsh1236I CGCG 1 cut(s) 130
BshFI GGCC 4 cut(s) 793, 1043, 1680, 1703
BsiHKAI GWGCWC 2 cut(s) 1069, 1286
BslFI GGGAC 2 cut(s) 543, 1429
BslI CCNNNNNNNGG 6 cut(s) 413, 525, 1184, 1276, 1413, 1449
BsmAI GTCTC 3 cut(s) 173, 444, 885
BsmFI GGGAC 2 cut(s) 543, 1429
BsmI GAATGC 1 cut(s) 461
BsnI GGCC 4 cut(s) 793, 1043, 1680, 1703
Bsp1286I GDGCHC 3 cut(s) 1069, 1144, 1286
Bsp143I GATC 7 cut(s) 161, 238, 419, 521, 660, 914, 1604
Bsp19I CCATGG 2 cut(s) 861, 1157
BspACI CCGC 2 cut(s) 436, 702
BspANI GGCC 4 cut(s) 793, 1043, 1680, 1703
BspCNI CTCAG 3 cut(s) 169, 371, 586
BspFNI CGCG 1 cut(s) 130
BspHI TCATGA 1 cut(s) 1299
BspLI GGNNCC 5 cut(s) 7, 615, 1189, 1548, 1557
BspMI ACCTGC 1 cut(s) 441
BspPI GGATC 4 cut(s) 246, 427, 922, 1612
BsrDI GCAATG 4 cut(s) 369, 852, 1233, 1506
BsrI ACTGG 6 cut(s) 53, 187, 298, 407, 1433, 1557
BssECI CCNNGG 4 cut(s) 9, 861, 1157, 1559
BssMI GATC 7 cut(s) 161, 238, 419, 521, 660, 914, 1604
BssSI CACGAG 1 cut(s) 1137
BssT1I CCWWGG 3 cut(s) 861, 1157, 1559
Bst2BI CACGAG 1 cut(s) 1137
Bst2UI CCWGG 3 cut(s) 232, 611, 1052
Bst4CI ACNGT 4 cut(s) 934, 1087, 1471, 1648
Bst6I CTCTTC 1 cut(s) 75
BstAPI GCANNNNNTGC 1 cut(s) 515
BstC8I GCNNGC 2 cut(s) 277, 1267
BstDEI CTNAG 3 cut(s) 177, 358, 594
BstDSI CCRYGG 2 cut(s) 861, 1157
BstEII GGTNACC 1 cut(s) 1527
BstF5I GGATG 4 cut(s) 698, 884, 1174, 1568
BstFNI CGCG 1 cut(s) 130
BstHHI GCGC 2 cut(s) 104, 132
BstKTI GATC 7 cut(s) 164, 241, 422, 524, 663, 917, 1607
BstMAI GTCTC 3 cut(s) 173, 444, 885
BstMBI GATC 7 cut(s) 161, 238, 419, 521, 660, 914, 1604
BstNI CCWGG 3 cut(s) 232, 611, 1052
BstPI GGTNACC 1 cut(s) 1527
BstSCI CCNGG 3 cut(s) 230, 609, 1050
BstSFI CTRYAG 1 cut(s) 639
BstUI CGCG 1 cut(s) 130
BstV1I GCAGC 5 cut(s) 521, 631, 802, 824, 1013
BstV2I GAAGAC 1 cut(s) 367
BstX2I RGATCY 3 cut(s) 238, 419, 1604
BstXI CCANNNNNNTGG 2 cut(s) 1051, 1539
BstYI RGATCY 3 cut(s) 238, 419, 1604
BsuRI GGCC 4 cut(s) 793, 1043, 1680, 1703
BtgI CCRYGG 2 cut(s) 861, 1157
BtgZI GCGATG 1 cut(s) 528
BtsCI GGATG 4 cut(s) 698, 884, 1174, 1568
BtsI GCAGTG 1 cut(s) 959
BtsIMutI CAGTG 7 cut(s) 384, 591, 959, 1083, 1512, 1550, 1644
BveI ACCTGC 1 cut(s) 441
Cac8I GCNNGC 2 cut(s) 277, 1267
CaiI CAGNNNCTG 2 cut(s) 413, 1475
CciI TCATGA 1 cut(s) 1299
CfoI GCGC 2 cut(s) 104, 132
Cfr13I GGNCC 6 cut(s) 145, 320, 613, 791, 1041, 1702
CseI GACGC 1 cut(s) 136
CsiI ACCWGGT 2 cut(s) 230, 1050
Csp6I GTAC 2 cut(s) 1048, 1120
CspCI CAANNNNNGTGG 2 cut(s) 1350, 1385
CviQI GTAC 2 cut(s) 1048, 1120
DdeI CTNAG 3 cut(s) 177, 358, 594
DpnI GATC 7 cut(s) 163, 240, 421, 523, 662, 916, 1606
DpnII GATC 7 cut(s) 161, 238, 419, 521, 660, 914, 1604
DraIII CACNNNGTG 1 cut(s) 234
Eam1104I CTCTTC 1 cut(s) 75
EarI CTCTTC 1 cut(s) 75
Eco130I CCWWGG 3 cut(s) 861, 1157, 1559
Eco24I GRGCYC 1 cut(s) 1144
Eco47I GGWCC 3 cut(s) 145, 320, 613
Eco57I CTGAAG 3 cut(s) 333, 888, 1404
Eco91I GGTNACC 1 cut(s) 1527
EcoO65I GGTNACC 1 cut(s) 1527
EcoRII CCWGG 3 cut(s) 230, 609, 1050
EcoT14I CCWWGG 3 cut(s) 861, 1157, 1559
EcoT38I GRGCYC 1 cut(s) 1144
ErhI CCWWGG 3 cut(s) 861, 1157, 1559
FaqI GGGAC 2 cut(s) 543, 1429
FbaI TGATCA 1 cut(s) 660
Fnu4HI GCNGC 5 cut(s) 510, 645, 813, 816, 1002
FokI GGATG 4 cut(s) 685, 871, 1161, 1555
FriOI GRGCYC 1 cut(s) 1144
Fsp4HI GCNGC 5 cut(s) 510, 645, 813, 816, 1002
FspBI CTAG 2 cut(s) 1322, 1376
GlaI GCGC 2 cut(s) 103, 131
GluI GCNGC 5 cut(s) 510, 645, 813, 816, 1002
GsuI CTGGAG 1 cut(s) 70
HaeIII GGCC 4 cut(s) 793, 1043, 1680, 1703
HgaI GACGC 1 cut(s) 136
HhaI GCGC 2 cut(s) 104, 132
Hin6I GCGC 2 cut(s) 102, 130
HinP1I GCGC 2 cut(s) 102, 130
HindIII AAGCTT 1 cut(s) 1249
HinfI GANTC 6 cut(s) 83, 150, 553, 1150, 1438, 1733
HphI GGTGA 7 cut(s) 35, 220, 1291, 1342, 1402, 1521, 1728
Hpy166II GTNNAC 2 cut(s) 826, 1384
Hpy188I TCNGA 2 cut(s) 10, 195
Hpy188III TCNNGA 7 cut(s) 488, 550, 888, 1210, 1300, 1369, 1442
Hpy8I GTNNAC 2 cut(s) 826, 1384
HpyAV CCTTC 2 cut(s) 1691, 1771
HpyCH4III ACNGT 4 cut(s) 934, 1087, 1471, 1648
HpyCH4IV ACGT 2 cut(s) 220, 1257
HpyF3I CTNAG 3 cut(s) 177, 358, 594
HpySE526I ACGT 2 cut(s) 220, 1257
HspAI GCGC 2 cut(s) 102, 130
Ksp22I TGATCA 1 cut(s) 660
Kzo9I GATC 7 cut(s) 161, 238, 419, 521, 660, 914, 1604
LmnI GCTCC 3 cut(s) 57, 99, 187
Lsp1109I GCAGC 5 cut(s) 521, 631, 802, 824, 1013
LweI GCATC 4 cut(s) 288, 707, 960, 1644
MabI ACCWGGT 2 cut(s) 230, 1050
MaeI CTAG 2 cut(s) 1322, 1376
MaeII ACGT 2 cut(s) 220, 1257
MaeIII GTNAC 4 cut(s) 120, 1348, 1465, 1527
MalI GATC 7 cut(s) 163, 240, 421, 523, 662, 916, 1606
MboI GATC 7 cut(s) 161, 238, 419, 521, 660, 914, 1604
MboII GAAGA 8 cut(s) 38, 92, 367, 881, 1082, 1288, 1395, 1608
MfeI CAATTG 1 cut(s) 819
MflI RGATCY 3 cut(s) 238, 419, 1604
MhlI GDGCHC 3 cut(s) 1069, 1144, 1286
MluCI AATT 5 cut(s) 18, 389, 651, 688, 819
MlyI GAGTC 2 cut(s) 92, 144
MmeI TCCRAC 2 cut(s) 77, 1430
MnlI CCTC 9 cut(s) 4, 236, 589, 688, 833, 1320, 1417, 1518, 1527
MseI TTAA 1 cut(s) 947
MslI CAYNNNNRTG 4 cut(s) 284, 312, 1304, 1649
MspA1I CMGCKG 1 cut(s) 815
MspR9I CCNGG 3 cut(s) 232, 611, 1052
MunI CAATTG 1 cut(s) 819
Mva1269I GAATGC 1 cut(s) 461
MvaI CCWGG 3 cut(s) 232, 611, 1052
MvnI CGCG 1 cut(s) 130
NcoI CCATGG 2 cut(s) 861, 1157
NdeII GATC 7 cut(s) 161, 238, 419, 521, 660, 914, 1604
NlaIV GGNNCC 5 cut(s) 7, 615, 1189, 1548, 1557
NmuCI GTSAC 4 cut(s) 120, 1348, 1465, 1527
OliI CACNNNNGTG 1 cut(s) 1649
PagI TCATGA 1 cut(s) 1299
PctI GAATGC 1 cut(s) 461
PfeI GAWTC 4 cut(s) 553, 1150, 1438, 1733
PflMI CCANNNNNTGG 2 cut(s) 413, 525
PfoI TCCNGGA 1 cut(s) 609
PkrI GCNGC 5 cut(s) 511, 646, 814, 817, 1003
PleI GAGTC 2 cut(s) 91, 144
PpsI GAGTC 2 cut(s) 91, 144
Psp1406I AACGTT 1 cut(s) 220
Psp6I CCWGG 3 cut(s) 230, 609, 1050
PspEI GGTNACC 1 cut(s) 1527
PspGI CCWGG 3 cut(s) 230, 609, 1050
PspN4I GGNNCC 5 cut(s) 7, 615, 1189, 1548, 1557
PspPI GGNCC 6 cut(s) 145, 320, 613, 791, 1041, 1702
PstNI CAGNNNCTG 2 cut(s) 413, 1475
PsuI RGATCY 3 cut(s) 238, 419, 1604
PvuII CAGCTG 1 cut(s) 815
RsaI GTAC 2 cut(s) 1049, 1121
RsaNI GTAC 2 cut(s) 1048, 1120
RseI CAYNNNNRTG 4 cut(s) 284, 312, 1304, 1649
SaqAI TTAA 1 cut(s) 947
SatI GCNGC 5 cut(s) 510, 645, 813, 816, 1002
Sau3AI GATC 7 cut(s) 161, 238, 419, 521, 660, 914, 1604
Sau96I GGNCC 6 cut(s) 145, 320, 613, 791, 1041, 1702
SchI GAGTC 2 cut(s) 92, 144
ScrFI CCNGG 3 cut(s) 232, 611, 1052
SduI GDGCHC 3 cut(s) 1069, 1144, 1286
SexAI ACCWGGT 2 cut(s) 230, 1050
SfaNI GCATC 4 cut(s) 288, 707, 960, 1644
SfcI CTRYAG 1 cut(s) 639
SinI GGWCC 3 cut(s) 145, 320, 613
SmiMI CAYNNNNRTG 4 cut(s) 284, 312, 1304, 1649
SmlI CTYRAG 2 cut(s) 488, 1070
SmoI CTYRAG 2 cut(s) 488, 1070
Sse9I AATT 5 cut(s) 18, 389, 651, 688, 819
SsiI CCGC 2 cut(s) 436, 702
SspI AATATT 2 cut(s) 723, 1591
SspMI CTAG 2 cut(s) 1322, 1376
StyD4I CCNGG 3 cut(s) 230, 609, 1050
StyI CCWWGG 3 cut(s) 861, 1157, 1559
TaaI ACNGT 4 cut(s) 934, 1087, 1471, 1648
TaiI ACGT 2 cut(s) 223, 1260
TaqI TCGA 1 cut(s) 399
TaqII GACCGA 1 cut(s) 162
TasI AATT 5 cut(s) 18, 389, 651, 688, 819
TfiI GAWTC 4 cut(s) 553, 1150, 1438, 1733
Tru1I TTAA 1 cut(s) 947
Tru9I TTAA 1 cut(s) 947
TscAI CASTG 7 cut(s) 391, 598, 966, 1090, 1512, 1557, 1651
TseFI GTSAC 4 cut(s) 120, 1348, 1465, 1527
TseI GCWGC 5 cut(s) 509, 644, 812, 815, 1001
Tsp45I GTSAC 4 cut(s) 120, 1348, 1465, 1527
TspDTI ATGAA 6 cut(s) 186, 230, 454, 785, 1288, 1673
TspRI CASTG 7 cut(s) 391, 598, 966, 1090, 1512, 1557, 1651
Van91I CCANNNNNTGG 2 cut(s) 413, 525
VpaK11BI GGWCC 3 cut(s) 145, 320, 613
XapI RAATTY 1 cut(s) 389
XcmI CCANNNNNNNNNTGG 3 cut(s) 45, 1090, 1557
XspI CTAG 2 cut(s) 1322, 1376
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.