Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr05

Orthogroup ID

OG0010657

Physical Location & Seq

Genomic Coordinates Forward (+)

40461027 .. 40463625

Gene Structure 2599 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD05G1269400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1335 bp

ATGAGAGAAATCCTCCACATCCAGGGAGGCCAGTGCGGCAACCAGATCGGCGCCAAGTTCTGGGAAGTGATCTGCGATGAGCACGGCATCGACAGCACCGGAAGGTACAACGGCGACTCCGAGCTCCAGCTCGAGCGAATCAATGTCTATTATAACGAGGCCACCGGAGGAAGGTACGTCCCACGTGCGGTGCTTATGGATCTGGAGCCTGGGACGATGGACGCGCTCAGATCCGGCCCGTTTGGGCAGATTTTCAGACCCGATAACTTCGTGTTCGGGCAGTCTGGGGCCGGGAACAATTGGGCTAAGGGGCATTATACGGAGGGCGCCGAGCTTATCGATTCCGTGCTCGATGTGGTGCGAAAAGAGGCTGAGAATTGCGATTGCTTGCAGGGATTCCAAGTATGTCATTCTTTGGGTGGTGGCACCGGCTCTGGCATGGGAACTCTTCTTATTTCCAAGATCAGAGAGGAGTACCCGGATCGAATGATGTTGACATTTTCTGTCTTCCCATCCCCAAAGGTGTCTGACACGGTTGTGGAGCCATACAATGCCACCCTTTCCGTCCACCAGCTTGTTGAAAATGCTGATGAATGTATGGTTTTGGACAATGAGGCTCTGTATGATATCTGCTTCCGCACCCTCAAGCTATCCAACCCTACTTTTGGTGATCTTAATCACCTCATCTCTGCTACCATGAGCGGTGTCACATGTTGTCTTCGGTTCCCTGGACAGTTGAACTCTGACCTACGCAAGCTTGCAGTTAACCTTATCCCTTTCCCACGGCTGCACTTCTTTATGGTTGGGTTTGCACCCCTAACTTCAAGAGGATCACAGCAGTACCGTTCTCTGTCTGTGCCCGAATTGACCCAGCAGATGTGGGATGCCAAAAACATGATGTGTGCTGCTGATCCACGCCATGGACGTTACCTTACTGCATCTGCTGTGTTCCGTGGTAAGATGAGCACCAAAGAAGTTGATGAACAGATGATTAATGTCCAGAACAAGAACTCCTCATACTTTGTCGAGTGGATTCCCAATAATGTTAAGTCCAGCGTGTGTGACATCCCACCCAAGGGTCTGAAAATGGCATCCACTTTCATTGGGAATTCGACTTCAATTCAGGAAATGTTCAGGAGAGTTAGCGAGCAGTTCACAGCTATGTTCAGGCGAAAGGCTTTCTTGCATTGGTACACGGGAGAGGGAATGGATGAGATGGAGTTCACCGAGGCTGAGAGTAACATGAATGATCTGGTTGCTGAGTACCAGCAATACCAGGATGCAACTATCTACGAAGAGGAGTACGAAGGGGAGGAAGAGGAGGTTGGTGCTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003729 GO:0005198 GO:0005200 GO:0005488 GO:0005575 GO:0005576 GO:0005618 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005774 GO:0005794 GO:0005829 GO:0005856 GO:0005886 GO:0005911 GO:0006996 GO:0007010 GO:0008150 GO:0009506 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009987 GO:0010035 GO:0010038 GO:0012505 GO:0016020 GO:0016043 GO:0030054 GO:0030312 GO:0031090 GO:0032991 GO:0042221 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0044422 GO:0044424 GO:0044430 GO:0044434 GO:0044435 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0045298 GO:0046686 GO:0048046 GO:0050896 GO:0055044 GO:0071840 GO:0071944 GO:0097159 GO:0098588 GO:0098805 GO:1901363

KEGG Pathways

Metabolic & Signaling

ko04145 ko04540 ko05130 map04145 map04540 map05130

Pfam Domains

Protein Families

Tubulin Tubulin_C

Protein Analysis

445

Amino Acids

49.91

Weight (kDa)

4.85

Isoelectric Point (pI)

39.02

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Tubulin	PF00091	3 - 211	4.3e-64	Tubulin/FtsZ family, GTPase domain
Tubulin_C	PF03953	261 - 382	3.1e-40	Tubulin C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0010657)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G20890 AT5G44340
fragaria_vesca	FvH4_3g10680
malus_domestica	MD05G1269400.v1.1
prunus_persica	Prupe.4G095300_v2.0.a1
rosa_chinensis	RchiOBHm_Chr5g0017391
rosa_laevigata	RLG00000031997 RLG00000032281 RLG00000033381
rosa_multiflora	Rmu_sc0005319.1_g000001 Rmu_sc0010664.1_g000003
rosa_roxburghii	Rroxscaffold_1G00059990
rosa_rugosa	Rorug05G0034800
rosa_samantha	Rh4AG140500 Rh4CG147400 Rh5AG102500 Rh5BG127700 Rh5CG110900
rosa_wichuraiana	Rw5G008950 Rw5G011210

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	153
AccB1I	GGYRCC	3 cut(s)	50, 326, 425
AccB7I	CCANNNNNTGG	2 cut(s)	60, 920
AccBSI	CCGCTC	1 cut(s)	702
AccII	CGCG	1 cut(s)	224
AciI	CCGC	4 cut(s)	36, 188, 637, 702
AclWI	GGATC	5 cut(s)	207, 225, 489, 838, 905
AcsI	RAATTY	1 cut(s)	1108
AcvI	CACGTG	1 cut(s)	185
AcyI	GRCGYC	2 cut(s)	51, 327
AfaI	GTAC	7 cut(s)	107, 176, 476, 842, 1193, 1265, 1304
AfiI	CCNNNNNNNGG	8 cut(s)	22, 60, 171, 187, 665, 920, 1075, 1076
AflIII	ACRYGT	1 cut(s)	710
AgsI	TTSAA	4 cut(s)	581, 739, 825, 1119
AjnI	CCWGG	4 cut(s)	21, 208, 727, 1275
AjuI	GAANNNNNNNTTGG	1 cut(s)	1308
AleI	CACNNNNGTG	1 cut(s)	536
AloI	GAACNNNNNNTCC	2 cut(s)	995, 1027
AluBI	AGCT	7 cut(s)	124, 130, 334, 574, 649, 757, 1160
AluI	AGCT	7 cut(s)	124, 130, 334, 574, 649, 757, 1160
Alw21I	GWGCWC	4 cut(s)	84, 126, 351, 968
AlwI	GGATC	5 cut(s)	207, 225, 489, 838, 905
Ama87I	CYCGRG	1 cut(s)	131
AoxI	GGCC	4 cut(s)	28, 159, 235, 288
ApeKI	GCWGC	2 cut(s)	787, 905
ApoI	RAATTY	1 cut(s)	1108
AseI	ATTAAT	1 cut(s)	993
AspLEI	GCGC	3 cut(s)	53, 226, 329
AspS9I	GGNCC	2 cut(s)	236, 288
AsuC2I	CCSGG	2 cut(s)	292, 479
AsuHPI	GGTGA	3 cut(s)	671, 680, 1216
AvaI	CYCGRG	1 cut(s)	131
BaeGI	GKGCMC	1 cut(s)	861
BaeI	ACNNNNGTAYC	2 cut(s)	824, 857
BanI	GGYRCC	3 cut(s)	50, 326, 425
BanII	GRGCYC	1 cut(s)	126
BbrPI	CACGTG	1 cut(s)	185
BbsI	GAAGAC	2 cut(s)	499, 710
Bbv12I	GWGCWC	4 cut(s)	84, 126, 351, 968
BbvI	GCAGC	2 cut(s)	774, 892
BccI	CCATC	3 cut(s)	211, 520, 1210
BceAI	ACGGC	3 cut(s)	100, 127, 800
BcgI	CGANNNNNNTGC	2 cut(s)	28, 62
BciT130I	CCWGG	4 cut(s)	23, 210, 729, 1277
BcnI	CCSGG	2 cut(s)	292, 479
BfoI	RGCGCY	2 cut(s)	54, 330
BglI	GCCNNNNNGGC	1 cut(s)	36
BisI	GCNGC	3 cut(s)	37, 788, 906
BlsI	GCNGC	3 cut(s)	38, 789, 907
Bme1390I	CCNGG	6 cut(s)	23, 210, 292, 479, 729, 1277
BmeT110I	CYCGRG	1 cut(s)	131
BmgT120I	GGNCC	2 cut(s)	236, 288
BmiI	GGNNCC	7 cut(s)	52, 207, 289, 328, 427, 543, 725
BmrFI	CCNGG	6 cut(s)	23, 210, 292, 479, 729, 1277
BmsI	GCATC	5 cut(s)	96, 874, 947, 1100, 1270
BpiI	GAAGAC	2 cut(s)	499, 710
BplI	GAGNNNNNCTC	1 cut(s)	29
BpmI	CTGGAG	2 cut(s)	110, 224
Bpu10I	CCTNAGC	1 cut(s)	306
BpuEI	CTTGAG	1 cut(s)	629
BpuMI	CCSGG	2 cut(s)	292, 479
Bsa29I	ATCGAT	1 cut(s)	339
BsaAI	YACGTR	1 cut(s)	185
BsaBI	GATNNNNATC	1 cut(s)	675
BsaHI	GRCGYC	2 cut(s)	51, 327
BsaJI	CCNNGG	8 cut(s)	22, 209, 727, 782, 919, 952, 1074, 1227
BsaWI	WCCGGW	2 cut(s)	98, 164
BsaXI	ACNNNNNCTCC	4 cut(s)	101, 131, 995, 1025
Bsc4I	CCNNNNNNNGG	8 cut(s)	22, 60, 171, 187, 665, 920, 1075, 1076
Bse118I	RCCGGY	1 cut(s)	428
Bse1I	ACTGG	1 cut(s)	31
Bse8I	GATNNNNATC	1 cut(s)	675
BseBI	CCWGG	4 cut(s)	23, 210, 729, 1277
BseCI	ATCGAT	1 cut(s)	339
BseDI	CCNNGG	8 cut(s)	22, 209, 727, 782, 919, 952, 1074, 1227
BseGI	GGATG	7 cut(s)	18, 512, 889, 1065, 1091, 1216, 1285
BseJI	GATNNNNATC	1 cut(s)	675
BseLI	CCNNNNNNNGG	8 cut(s)	22, 60, 171, 187, 665, 920, 1075, 1076
BseMII	CTCAG	4 cut(s)	241, 363, 1224, 1251
BseNI	ACTGG	1 cut(s)	31
BseRI	GAGGAG	4 cut(s)	485, 1003, 1313, 1334
BseSI	GKGCMC	1 cut(s)	861
BseXI	GCAGC	2 cut(s)	774, 892
BseYI	CCCAGC	1 cut(s)	870
BsgI	GTGCAG	1 cut(s)	773
Bsh1236I	CGCG	1 cut(s)	224
BshFI	GGCC	4 cut(s)	30, 161, 237, 290
BshNI	GGYRCC	3 cut(s)	50, 326, 425
BshVI	ATCGAT	1 cut(s)	339
BsiHKAI	GWGCWC	4 cut(s)	84, 126, 351, 968
BsiHKCI	CYCGRG	1 cut(s)	131
BsiSI	CCGG	6 cut(s)	99, 165, 234, 291, 429, 479
BslFI	GGGAC	2 cut(s)	164, 226
BslI	CCNNNNNNNGG	8 cut(s)	22, 60, 171, 187, 665, 920, 1075, 1076
BsmFI	GGGAC	2 cut(s)	164, 226
BsnI	GGCC	4 cut(s)	30, 161, 237, 290
BsoBI	CYCGRG	1 cut(s)	131
Bsp1286I	GDGCHC	5 cut(s)	84, 126, 351, 861, 968
Bsp19I	CCATGG	1 cut(s)	919
BspACI	CCGC	4 cut(s)	36, 188, 637, 702
BspANI	GGCC	4 cut(s)	30, 161, 237, 290
BspCNI	CTCAG	4 cut(s)	240, 364, 1225, 1252
BspDI	ATCGAT	1 cut(s)	339
BspFNI	CGCG	1 cut(s)	224
BspLI	GGNNCC	7 cut(s)	52, 207, 289, 328, 427, 543, 725
BspPI	GGATC	5 cut(s)	207, 225, 489, 838, 905
BspT107I	GGYRCC	3 cut(s)	50, 326, 425
BsrBI	CCGCTC	1 cut(s)	702
BsrFI	RCCGGY	1 cut(s)	428
BsrI	ACTGG	1 cut(s)	31
BssAI	RCCGGY	1 cut(s)	428
BssECI	CCNNGG	8 cut(s)	22, 209, 727, 782, 919, 952, 1074, 1227
BssNI	GRCGYC	2 cut(s)	51, 327
BssT1I	CCWWGG	2 cut(s)	919, 1074
Bst2UI	CCWGG	4 cut(s)	23, 210, 729, 1277
Bst4CI	ACNGT	3 cut(s)	535, 735, 845
Bst6I	CTCTTC	3 cut(s)	453, 1290, 1311
BstACI	GRCGYC	2 cut(s)	51, 327
BstBAI	YACGTR	1 cut(s)	185
BstC8I	GCNNGC	4 cut(s)	389, 755, 759, 1148
BstDEI	CTNAG	5 cut(s)	227, 306, 372, 1233, 1260
BstDSI	CCRYGG	3 cut(s)	782, 919, 952
BstF5I	GGATG	7 cut(s)	18, 512, 889, 1065, 1091, 1216, 1285
BstFNI	CGCG	1 cut(s)	224
BstH2I	RGCGCY	2 cut(s)	54, 330
BstHHI	GCGC	3 cut(s)	53, 226, 329
BstMWI	GCNNNNNNNGC	2 cut(s)	36, 93
BstNI	CCWGG	4 cut(s)	23, 210, 729, 1277
BstNSI	RCATGY	1 cut(s)	714
BstSCI	CCNGG	6 cut(s)	21, 208, 290, 477, 727, 1275
BstSLI	GKGCMC	1 cut(s)	861
BstUI	CGCG	1 cut(s)	224
BstV1I	GCAGC	2 cut(s)	774, 892
BstV2I	GAAGAC	2 cut(s)	499, 710
BstX2I	RGATCY	2 cut(s)	199, 230
BstYI	RGATCY	2 cut(s)	199, 230
Bsu15I	ATCGAT	1 cut(s)	339
BsuRI	GGCC	4 cut(s)	30, 161, 237, 290
BsuTUI	ATCGAT	1 cut(s)	339
BtgI	CCRYGG	3 cut(s)	782, 919, 952
BtgZI	GCGATG	1 cut(s)	90
BtsCI	GGATG	7 cut(s)	18, 512, 889, 1065, 1091, 1216, 1285
BtsIMutI	CAGTG	1 cut(s)	38
Cac8I	GCNNGC	4 cut(s)	389, 755, 759, 1148
CfoI	GCGC	3 cut(s)	53, 226, 329
Cfr10I	RCCGGY	1 cut(s)	428
Cfr13I	GGNCC	2 cut(s)	236, 288
ClaI	ATCGAT	1 cut(s)	339
CseI	GACGC	1 cut(s)	230
Csp6I	GTAC	7 cut(s)	106, 175, 475, 841, 1192, 1264, 1303
CspCI	CAANNNNNGTGG	2 cut(s)	1084, 1119
CviAII	CATG	6 cut(s)	439, 697, 711, 895, 920, 1243
CviQI	GTAC	7 cut(s)	106, 175, 475, 841, 1192, 1264, 1303
DdeI	CTNAG	5 cut(s)	227, 306, 372, 1233, 1260
DinI	GGCGCC	2 cut(s)	52, 328
Eam1104I	CTCTTC	3 cut(s)	453, 1290, 1311
EarI	CTCTTC	3 cut(s)	453, 1290, 1311
Ecl136II	GAGCTC	1 cut(s)	124
Eco130I	CCWWGG	2 cut(s)	919, 1074
Eco24I	GRGCYC	1 cut(s)	126
Eco32I	GATATC	1 cut(s)	628
Eco53kI	GAGCTC	1 cut(s)	124
Eco72I	CACGTG	1 cut(s)	185
Eco88I	CYCGRG	1 cut(s)	131
EcoICRI	GAGCTC	1 cut(s)	124
EcoRI	GAATTC	1 cut(s)	1108
EcoRII	CCWGG	4 cut(s)	21, 208, 727, 1275
EcoRV	GATATC	1 cut(s)	628
EcoT14I	CCWWGG	2 cut(s)	919, 1074
EcoT38I	GRGCYC	1 cut(s)	126
EgeI	GGCGCC	2 cut(s)	52, 328
EheI	GGCGCC	2 cut(s)	52, 328
ErhI	CCWWGG	2 cut(s)	919, 1074
FaeI	CATG	6 cut(s)	442, 700, 714, 898, 923, 1246
FalI	AAGNNNNNCTT	2 cut(s)	1166, 1198
FaqI	GGGAC	2 cut(s)	164, 226
FatI	CATG	6 cut(s)	438, 696, 710, 894, 919, 1242
Fnu4HI	GCNGC	3 cut(s)	37, 788, 906
FokI	GGATG	7 cut(s)	5, 499, 896, 1052, 1078, 1223, 1292
FriOI	GRGCYC	1 cut(s)	126
Fsp4HI	GCNGC	3 cut(s)	37, 788, 906
GlaI	GCGC	3 cut(s)	52, 225, 328
GluI	GCNGC	3 cut(s)	37, 788, 906
GsaI	CCCAGC	1 cut(s)	874
GsuI	CTGGAG	2 cut(s)	110, 224
HaeII	RGCGCY	2 cut(s)	54, 330
HaeIII	GGCC	4 cut(s)	30, 161, 237, 290
HapII	CCGG	6 cut(s)	99, 165, 234, 291, 429, 479
HgaI	GACGC	1 cut(s)	230
HhaI	GCGC	3 cut(s)	53, 226, 329
Hin1I	GRCGYC	2 cut(s)	51, 327
Hin1II	CATG	6 cut(s)	442, 700, 714, 898, 923, 1246
Hin6I	GCGC	3 cut(s)	51, 224, 327
HinP1I	GCGC	3 cut(s)	51, 224, 327
HincII	GTYRAC	2 cut(s)	495, 766
HindII	GTYRAC	2 cut(s)	495, 766
HindIII	AAGCTT	1 cut(s)	755
HinfI	GANTC	5 cut(s)	116, 138, 341, 396, 1033
HpaI	GTTAAC	1 cut(s)	766
HpaII	CCGG	6 cut(s)	99, 165, 234, 291, 429, 479
HphI	GGTGA	3 cut(s)	671, 680, 1216
Hpy166II	GTNNAC	6 cut(s)	495, 568, 766, 1155, 1194, 1224
Hpy188I	TCNGA	7 cut(s)	121, 230, 257, 467, 529, 745, 1083
Hpy188III	TCNNGA	5 cut(s)	203, 825, 1000, 1124, 1135
Hpy8I	GTNNAC	6 cut(s)	495, 568, 766, 1155, 1194, 1224
HpyAV	CCTTC	3 cut(s)	96, 165, 1301
HpyCH4III	ACNGT	3 cut(s)	535, 735, 845
HpyCH4IV	ACGT	3 cut(s)	177, 184, 925
HpyCH4V	TGCA	7 cut(s)	391, 761, 790, 812, 938, 1186, 1283
HpyF10VI	GCNNNNNNNGC	2 cut(s)	36, 93
HpyF3I	CTNAG	5 cut(s)	227, 306, 372, 1233, 1260
HpySE526I	ACGT	3 cut(s)	177, 184, 925
Hsp92I	GRCGYC	2 cut(s)	51, 327
Hsp92II	CATG	6 cut(s)	442, 700, 714, 898, 923, 1246
HspAI	GCGC	3 cut(s)	51, 224, 327
KasI	GGCGCC	2 cut(s)	50, 326
KspAI	GTTAAC	1 cut(s)	766
LmnI	GCTCC	3 cut(s)	129, 205, 541
Lsp1109I	GCAGC	2 cut(s)	774, 892
LweI	GCATC	5 cut(s)	96, 874, 947, 1100, 1270
MaeII	ACGT	3 cut(s)	177, 184, 925
MaeIII	GTNAC	4 cut(s)	706, 926, 1061, 1238
MbiI	CCGCTC	1 cut(s)	702
MboII	GAAGA	5 cut(s)	440, 499, 710, 1307, 1328
MfeI	CAATTG	1 cut(s)	298
MflI	RGATCY	2 cut(s)	199, 230
MhlI	GDGCHC	5 cut(s)	84, 126, 351, 861, 968
MluCI	AATT	5 cut(s)	298, 376, 863, 1108, 1119
Mly113I	GGCGCC	2 cut(s)	51, 327
MlyI	GAGTC	1 cut(s)	110
MmeI	TCCRAC	1 cut(s)	678
MseI	TTAA	4 cut(s)	675, 765, 993, 1047
MslI	CAYNNNNRTG	2 cut(s)	536, 1160
MspI	CCGG	6 cut(s)	99, 165, 234, 291, 429, 479
MspR9I	CCNGG	6 cut(s)	23, 210, 292, 479, 729, 1277
MunI	CAATTG	1 cut(s)	298
MvaI	CCWGG	4 cut(s)	23, 210, 729, 1277
MvnI	CGCG	1 cut(s)	224
MwoI	GCNNNNNNNGC	2 cut(s)	36, 93
NarI	GGCGCC	2 cut(s)	51, 327
NciI	CCSGG	2 cut(s)	292, 479
NcoI	CCATGG	1 cut(s)	919
NlaIII	CATG	6 cut(s)	442, 700, 714, 898, 923, 1246
NlaIV	GGNNCC	7 cut(s)	52, 207, 289, 328, 427, 543, 725
NmeAIII	GCCGAG	1 cut(s)	355
NmuCI	GTSAC	2 cut(s)	706, 1061
NspI	RCATGY	1 cut(s)	714
OliI	CACNNNNGTG	1 cut(s)	536
PaeR7I	CTCGAG	1 cut(s)	131
PciI	ACATGT	1 cut(s)	710
PcsI	WCGNNNNNNNCGW	2 cut(s)	117, 922
PfeI	GAWTC	4 cut(s)	138, 341, 396, 1033
PflMI	CCANNNNNTGG	2 cut(s)	60, 920
PkrI	GCNGC	3 cut(s)	38, 789, 907
PleI	GAGTC	1 cut(s)	110
PluTI	GGCGCC	2 cut(s)	54, 330
PmaCI	CACGTG	1 cut(s)	185
PmlI	CACGTG	1 cut(s)	185
PpsI	GAGTC	1 cut(s)	110
Ppu21I	YACGTR	1 cut(s)	185
PscI	ACATGT	1 cut(s)	710
PshBI	ATTAAT	1 cut(s)	993
PsiI	TTATAA	1 cut(s)	153
Psp124BI	GAGCTC	1 cut(s)	126
Psp6I	CCWGG	4 cut(s)	21, 208, 727, 1275
PspCI	CACGTG	1 cut(s)	185
PspFI	CCCAGC	1 cut(s)	870
PspGI	CCWGG	4 cut(s)	21, 208, 727, 1275
PspN4I	GGNNCC	7 cut(s)	52, 207, 289, 328, 427, 543, 725
PspPI	GGNCC	2 cut(s)	236, 288
PspXI	VCTCGAGB	1 cut(s)	131
PsrI	GAACNNNNNNTAC	2 cut(s)	1001, 1033
PsuI	RGATCY	2 cut(s)	199, 230
RsaI	GTAC	7 cut(s)	107, 176, 476, 842, 1193, 1265, 1304
RsaNI	GTAC	7 cut(s)	106, 175, 475, 841, 1192, 1264, 1303
RseI	CAYNNNNRTG	2 cut(s)	536, 1160
SacI	GAGCTC	1 cut(s)	126
SaqAI	TTAA	4 cut(s)	675, 765, 993, 1047
SatI	GCNGC	3 cut(s)	37, 788, 906
Sau96I	GGNCC	2 cut(s)	236, 288
SchI	GAGTC	1 cut(s)	110
ScrFI	CCNGG	6 cut(s)	23, 210, 292, 479, 729, 1277
SduI	GDGCHC	5 cut(s)	84, 126, 351, 861, 968
SfaNI	GCATC	5 cut(s)	96, 874, 947, 1100, 1270
SfoI	GGCGCC	2 cut(s)	52, 328
Sfr274I	CTCGAG	1 cut(s)	131
SlaI	CTCGAG	1 cut(s)	131
SmiMI	CAYNNNNRTG	2 cut(s)	536, 1160
SmlI	CTYRAG	2 cut(s)	131, 644
SmoI	CTYRAG	2 cut(s)	131, 644
Sse9I	AATT	5 cut(s)	298, 376, 863, 1108, 1119
SsiI	CCGC	4 cut(s)	36, 188, 637, 702
SspDI	GGCGCC	2 cut(s)	50, 326
SstI	GAGCTC	1 cut(s)	126
StyD4I	CCNGG	6 cut(s)	21, 208, 290, 477, 727, 1275
StyI	CCWWGG	2 cut(s)	919, 1074
TaaI	ACNGT	3 cut(s)	535, 735, 845
TaiI	ACGT	3 cut(s)	180, 187, 928
TaqI	TCGA	7 cut(s)	90, 132, 339, 351, 484, 1026, 1112
TasI	AATT	5 cut(s)	298, 376, 863, 1108, 1119
TauI	GCSGC	1 cut(s)	39
TfiI	GAWTC	4 cut(s)	138, 341, 396, 1033
Tru1I	TTAA	4 cut(s)	675, 765, 993, 1047
Tru9I	TTAA	4 cut(s)	675, 765, 993, 1047
TscAI	CASTG	1 cut(s)	38
TseFI	GTSAC	2 cut(s)	706, 1061
TseI	GCWGC	2 cut(s)	787, 905
Tsp45I	GTSAC	2 cut(s)	706, 1061
TspDTI	ATGAA	4 cut(s)	606, 996, 1090, 1259
TspGWI	ACGGA	4 cut(s)	334, 335, 553, 941
TspRI	CASTG	1 cut(s)	38
Van91I	CCANNNNNTGG	2 cut(s)	60, 920
VspI	ATTAAT	1 cut(s)	993
XapI	RAATTY	1 cut(s)	1108
XceI	RCATGY	1 cut(s)	714
XhoI	CTCGAG	1 cut(s)	131

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.