RLG00000033381

Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0010657
Physical Location & Seq
Reverse (-)
26473545 .. 26479295
5751 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000033381

Sequence Viewer

Length: 960 bp
ATGGCTGATCGTTGTAGACAATTGCATGGTGACAACCGAAAGCCTCACAATGTTGAGAAACCTTCCAGAGTTTCAAGGCTGGTGGTTCACCAGGAGTATAGGGTGAAGGATAAGGTTCCCTCCACAATCCAGACTAAGGAGATTCAACCATTAGCTAAGTCAACTGAAATTAATATTATGTCTCCATCATGTACACTGATTGGTACAATGCAGGTGCAAGGTGACCAAGATCCTATACAAGTTTCCAGGAGAAATAGAGAGCATAGTGACAATGGGAGAGCTAGCAGTCCAGGAGGTGATGTAGCCACTGAGAGACCGGGAACTCCTAATAATGTTTCCACGGAGAATCAGTTCACCATTTTGGCTCAAGAAGTCATTGCGGGGGCGGCCAACGATTTAATTGAGCAATTGGCAGACCAAGTATTTCTTGAGCCAAATAATCAGCCTTCAGAGCATTCTAGGCTGGTTGTTGTTGAGGCTTCAGATGATGAAATTGATTCTTTCCCCACCTCCCTAATGGTGACGGAGGGAGATGAAGTTGTTGTTCAGGGGCAGGAAGATGATATGACTATTGTTCTTTCTAAATCGCCAAAAAAGAAGAAAAAGAAGAAAATGCTTGAGGAAAAATTACGGGAGCAAGCTTCCGACCGTTATCCTACCTGGTTTCAGGTGTGCCATTCGCTTGGTGGAGGAACCGGATCTGGTATGGGGACTCTGTTGATTTCGAAAATCAGGGAGGAATACCCTGACAGGATGATGCTCACTTTCTCTGTGTTTCCATCACCAAAAGTCTCCGATATGGTGGTTGAGCCCTATAATGCTACCTTGTCTGTGCACCAGCTTGTTGAGAATGCTGATGAGTGTATGGTTCTCGATAACGAAGCTCTCTATGACATTTGCTTCCGCACACTCAAGCTCAGTACCCCAAGCTGTAAGATCCTATTGATTGTGTTGGTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04145 ko04540 ko05130 map04145 map04540 map05130
Pfam Domains
Protein Families
Tubulin Tubulin_C

Protein Analysis

320

Amino Acids

35.55

Weight (kDa)

5.3

Isoelectric Point (pI)

47.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Tubulin PF00091 138 - 300 7.7e-28 Tubulin/FtsZ family, GTPase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 202
Acc36I ACCTGC 1 cut(s) 202
AccI GTMKAC 1 cut(s) 16
AciI CCGC 3 cut(s) 380, 386, 904
AclWI GGATC 3 cut(s) 224, 706, 931
AcoI YGGCCR 1 cut(s) 387
AcuI CTGAAG 2 cut(s) 432, 465
AfaI GTAC 3 cut(s) 193, 205, 922
AfiI CCNNNNNNNGG 1 cut(s) 136
AgsI TTSAA 2 cut(s) 75, 146
AjnI CCWGG 4 cut(s) 90, 245, 289, 659
AloI GAACNNNNNNTCC 2 cut(s) 335, 367
AluBI AGCT 7 cut(s) 155, 281, 641, 841, 884, 916, 930
AluI AGCT 7 cut(s) 155, 281, 641, 841, 884, 916, 930
Alw21I GWGCWC 1 cut(s) 837
Alw26I GTCTC 3 cut(s) 186, 307, 796
Alw44I GTGCAC 1 cut(s) 833
AlwI GGATC 3 cut(s) 224, 706, 931
AoxI GGCC 1 cut(s) 387
ApaLI GTGCAC 1 cut(s) 833
AseI ATTAAT 1 cut(s) 171
Asp700I GAANNNNTTC 1 cut(s) 350
AsuC2I CCSGG 1 cut(s) 318
AsuHPI GGTGA 8 cut(s) 41, 80, 115, 233, 308, 346, 532, 774
AsuII TTCGAA 1 cut(s) 725
AsuNHI GCTAGC 1 cut(s) 281
BaeGI GKGCMC 1 cut(s) 837
BanII GRGCYC 1 cut(s) 813
Bbv12I GWGCWC 1 cut(s) 837
BccI CCATC 2 cut(s) 193, 787
BciT130I CCWGG 4 cut(s) 92, 247, 291, 661
BcnI CCSGG 1 cut(s) 318
BcoDI GTCTC 3 cut(s) 186, 307, 796
BfaI CTAG 2 cut(s) 282, 459
BfuAI ACCTGC 1 cut(s) 202
BisI GCNGC 1 cut(s) 387
BlsI GCNGC 1 cut(s) 388
Bme1390I CCNGG 5 cut(s) 92, 247, 291, 318, 661
BmiI GGNNCC 2 cut(s) 117, 694
BmrFI CCNGG 5 cut(s) 92, 247, 291, 318, 661
BmsI GCATC 1 cut(s) 747
BmtI GCTAGC 1 cut(s) 285
Bpu14I TTCGAA 1 cut(s) 725
BpuEI CTTGAG 4 cut(s) 351, 449, 638, 896
BpuMI CCSGG 1 cut(s) 318
BsaI GGTCTC 1 cut(s) 307
BsaJI CCNNGG 1 cut(s) 339
BsaWI WCCGGW 1 cut(s) 695
BsaXI ACNNNNNCTCC 6 cut(s) 285, 315, 335, 365, 522, 552
Bsc4I CCNNNNNNNGG 1 cut(s) 136
Bse3DI GCAATG 1 cut(s) 375
BseBI CCWGG 4 cut(s) 92, 247, 291, 661
BseDI CCNNGG 1 cut(s) 339
BseGI GGATG 1 cut(s) 759
BseLI CCNNNNNNNGG 1 cut(s) 136
BseMI GCAATG 1 cut(s) 375
BseMII CTCAG 2 cut(s) 300, 931
BseSI GKGCMC 1 cut(s) 837
Bsh1285I CGRYCG 1 cut(s) 649
BshFI GGCC 1 cut(s) 389
BsiEI CGRYCG 1 cut(s) 649
BsiHKAI GWGCWC 1 cut(s) 837
BsiSI CCGG 2 cut(s) 317, 696
BslFI GGGAC 1 cut(s) 724
BslI CCNNNNNNNGG 1 cut(s) 136
BsmAI GTCTC 3 cut(s) 186, 307, 796
BsmFI GGGAC 1 cut(s) 724
BsmI GAATGC 2 cut(s) 454, 856
BsnI GGCC 1 cut(s) 389
Bso31I GGTCTC 1 cut(s) 307
Bsp119I TTCGAA 1 cut(s) 725
Bsp1286I GDGCHC 2 cut(s) 813, 837
Bsp1407I TGTACA 1 cut(s) 191
Bsp143I GATC 4 cut(s) 7, 229, 698, 936
BspACI CCGC 3 cut(s) 380, 386, 904
BspANI GGCC 1 cut(s) 389
BspCNI CTCAG 2 cut(s) 301, 930
BspLI GGNNCC 2 cut(s) 117, 694
BspMI ACCTGC 1 cut(s) 202
BspOI GCTAGC 1 cut(s) 285
BspPI GGATC 3 cut(s) 224, 706, 931
BspT104I TTCGAA 1 cut(s) 725
BspTNI GGTCTC 1 cut(s) 307
BsrDI GCAATG 1 cut(s) 375
BsrGI TGTACA 1 cut(s) 191
BssECI CCNNGG 1 cut(s) 339
BssMI GATC 4 cut(s) 7, 229, 698, 936
Bst2UI CCWGG 4 cut(s) 92, 247, 291, 661
Bst4CI ACNGT 1 cut(s) 650
BstAUI TGTACA 1 cut(s) 191
BstBI TTCGAA 1 cut(s) 725
BstC8I GCNNGC 2 cut(s) 283, 639
BstDEI CTNAG 4 cut(s) 135, 156, 309, 917
BstDSI CCRYGG 1 cut(s) 339
BstEII GGTNACC 1 cut(s) 221
BstF5I GGATG 1 cut(s) 759
BstKTI GATC 4 cut(s) 10, 232, 701, 939
BstMAI GTCTC 3 cut(s) 186, 307, 796
BstMBI GATC 4 cut(s) 7, 229, 698, 936
BstMCI CGRYCG 1 cut(s) 649
BstMWI GCNNNNNNNGC 3 cut(s) 386, 451, 460
BstNI CCWGG 4 cut(s) 92, 247, 291, 661
BstPI GGTNACC 1 cut(s) 221
BstSCI CCNGG 5 cut(s) 90, 245, 289, 316, 659
BstSLI GKGCMC 1 cut(s) 837
BstX2I RGATCY 3 cut(s) 229, 698, 936
BstXI CCANNNNNNTGG 1 cut(s) 683
BstYI RGATCY 3 cut(s) 229, 698, 936
BsuRI GGCC 1 cut(s) 389
BtgI CCRYGG 1 cut(s) 339
BtsCI GGATG 1 cut(s) 759
BtsIMutI CAGTG 2 cut(s) 194, 306
BveI ACCTGC 1 cut(s) 202
Cac8I GCNNGC 2 cut(s) 283, 639
CsiI ACCWGGT 1 cut(s) 659
Csp6I GTAC 3 cut(s) 192, 204, 921
CspCI CAANNNNNGTGG 2 cut(s) 63, 98
CviAII CATG 2 cut(s) 26, 189
CviQI GTAC 3 cut(s) 192, 204, 921
DdeI CTNAG 4 cut(s) 135, 156, 309, 917
DpnI GATC 4 cut(s) 9, 231, 700, 938
DpnII GATC 4 cut(s) 7, 229, 698, 936
EaeI YGGCCR 1 cut(s) 387
Eco24I GRGCYC 1 cut(s) 813
Eco31I GGTCTC 1 cut(s) 307
Eco57I CTGAAG 2 cut(s) 432, 465
Eco91I GGTNACC 1 cut(s) 221
EcoO65I GGTNACC 1 cut(s) 221
EcoRII CCWGG 4 cut(s) 90, 245, 289, 659
EcoT38I GRGCYC 1 cut(s) 813
FaeI CATG 2 cut(s) 29, 192
FalI AAGNNNNNCTT 2 cut(s) 411, 443
FaqI GGGAC 1 cut(s) 724
FatI CATG 2 cut(s) 25, 188
FauI CCCGC 1 cut(s) 373
FblI GTMKAC 1 cut(s) 16
Fnu4HI GCNGC 1 cut(s) 387
FokI GGATG 1 cut(s) 766
FriOI GRGCYC 1 cut(s) 813
Fsp4HI GCNGC 1 cut(s) 387
FspBI CTAG 2 cut(s) 282, 459
GluI GCNGC 1 cut(s) 387
HaeIII GGCC 1 cut(s) 389
HapII CCGG 2 cut(s) 317, 696
Hin1II CATG 2 cut(s) 29, 192
HincII GTYRAC 1 cut(s) 162
HindII GTYRAC 1 cut(s) 162
HindIII AAGCTT 1 cut(s) 639
HinfI GANTC 4 cut(s) 142, 346, 497, 712
HpaII CCGG 2 cut(s) 317, 696
HphI GGTGA 8 cut(s) 41, 80, 115, 233, 308, 346, 532, 774
Hpy166II GTNNAC 6 cut(s) 17, 88, 162, 194, 354, 835
Hpy188I TCNGA 4 cut(s) 451, 484, 646, 796
Hpy188III TCNNGA 5 cut(s) 66, 130, 368, 428, 872
Hpy8I GTNNAC 6 cut(s) 17, 88, 162, 194, 354, 835
HpyAV CCTTC 3 cut(s) 72, 100, 456
HpyCH4III ACNGT 1 cut(s) 650
HpyCH4V TGCA 4 cut(s) 25, 211, 217, 835
HpyF10VI GCNNNNNNNGC 3 cut(s) 386, 451, 460
HpyF3I CTNAG 4 cut(s) 135, 156, 309, 917
Hsp92II CATG 2 cut(s) 29, 192
Kzo9I GATC 4 cut(s) 7, 229, 698, 936
LmnI GCTCC 1 cut(s) 634
LweI GCATC 1 cut(s) 747
MabI ACCWGGT 1 cut(s) 659
MaeI CTAG 2 cut(s) 282, 459
MaeIII GTNAC 4 cut(s) 29, 221, 266, 520
MalI GATC 4 cut(s) 9, 231, 700, 938
MboI GATC 4 cut(s) 7, 229, 698, 936
MboII GAAGA 3 cut(s) 569, 610, 619
MfeI CAATTG 2 cut(s) 20, 407
MflI RGATCY 3 cut(s) 229, 698, 936
MhlI GDGCHC 2 cut(s) 813, 837
MluCI AATT 6 cut(s) 20, 168, 399, 407, 492, 626
MlyI GAGTC 1 cut(s) 706
MmeI TCCRAC 1 cut(s) 669
MnlI CCTC 9 cut(s) 54, 130, 287, 469, 520, 520, 613, 683, 730
MroXI GAANNNNTTC 1 cut(s) 350
MseI TTAA 2 cut(s) 171, 398
MspI CCGG 2 cut(s) 317, 696
MspR9I CCNGG 5 cut(s) 92, 247, 291, 318, 661
MunI CAATTG 2 cut(s) 20, 407
Mva1269I GAATGC 2 cut(s) 454, 856
MvaI CCWGG 4 cut(s) 92, 247, 291, 661
MwoI GCNNNNNNNGC 3 cut(s) 386, 451, 460
NciI CCSGG 1 cut(s) 318
NdeII GATC 4 cut(s) 7, 229, 698, 936
NheI GCTAGC 1 cut(s) 281
NlaIII CATG 2 cut(s) 29, 192
NlaIV GGNNCC 2 cut(s) 117, 694
NmuCI GTSAC 4 cut(s) 29, 221, 266, 520
NspV TTCGAA 1 cut(s) 725
PaqCI CACCTGC 1 cut(s) 202
PctI GAATGC 2 cut(s) 454, 856
PdmI GAANNNNTTC 1 cut(s) 350
PfeI GAWTC 3 cut(s) 142, 346, 497
PfoI TCCNGGA 2 cut(s) 245, 289
PkrI GCNGC 1 cut(s) 388
PleI GAGTC 1 cut(s) 706
PpsI GAGTC 1 cut(s) 706
PshBI ATTAAT 1 cut(s) 171
Psp6I CCWGG 4 cut(s) 90, 245, 289, 659
PspEI GGTNACC 1 cut(s) 221
PspGI CCWGG 4 cut(s) 90, 245, 289, 659
PspN4I GGNNCC 2 cut(s) 117, 694
PsuI RGATCY 3 cut(s) 229, 698, 936
RsaI GTAC 3 cut(s) 193, 205, 922
RsaNI GTAC 3 cut(s) 192, 204, 921
SaqAI TTAA 2 cut(s) 171, 398
SatI GCNGC 1 cut(s) 387
Sau3AI GATC 4 cut(s) 7, 229, 698, 936
SchI GAGTC 1 cut(s) 706
ScrFI CCNGG 5 cut(s) 92, 247, 291, 318, 661
SduI GDGCHC 2 cut(s) 813, 837
SexAI ACCWGGT 1 cut(s) 659
SfaNI GCATC 1 cut(s) 747
SfuI TTCGAA 1 cut(s) 725
SmlI CTYRAG 4 cut(s) 366, 428, 617, 911
SmoI CTYRAG 4 cut(s) 366, 428, 617, 911
Sse9I AATT 6 cut(s) 20, 168, 399, 407, 492, 626
SsiI CCGC 3 cut(s) 380, 386, 904
SspI AATATT 1 cut(s) 175
SspMI CTAG 2 cut(s) 282, 459
StyD4I CCNGG 5 cut(s) 90, 245, 289, 316, 659
TaaI ACNGT 1 cut(s) 650
TaqI TCGA 2 cut(s) 725, 873
TasI AATT 6 cut(s) 20, 168, 399, 407, 492, 626
TatI WGTACW 1 cut(s) 191
TauI GCSGC 1 cut(s) 389
TfiI GAWTC 3 cut(s) 142, 346, 497
Tru1I TTAA 2 cut(s) 171, 398
Tru9I TTAA 2 cut(s) 171, 398
TscAI CASTG 2 cut(s) 201, 313
TseFI GTSAC 4 cut(s) 29, 221, 266, 520
Tsp45I GTSAC 4 cut(s) 29, 221, 266, 520
TspDTI ATGAA 2 cut(s) 504, 549
TspGWI ACGGA 2 cut(s) 356, 539
TspRI CASTG 2 cut(s) 201, 313
VneI GTGCAC 1 cut(s) 833
VspI ATTAAT 1 cut(s) 171
XcmI CCANNNNNNNNNTGG 2 cut(s) 514, 683
XmiI GTMKAC 1 cut(s) 16
XmnI GAANNNNTTC 1 cut(s) 350
XspI CTAG 2 cut(s) 282, 459
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.