Rmu_sc0010664.1_g000003

Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0010664.1
OG0010657
Physical Location & Seq
Reverse (-)
10062 .. 11131
1070 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0010664.1_g000003.1.cds

Sequence Viewer

Length: 819 bp
atggccgaagctcaagaatccgaccaaaagagtgaagaagaagaagaagaggaacactatggaagccgaagctcaagaatcgagcccccaaaacgacgccctcatcacctccttctgcgagatcacctccacctccaaacaagaagccctcttcttcctcgaattccacaattacgacctcgacgacgccgccgccgtcaccttcaccaacccggtccagccctcccactcgctctcgccgtctccgtcacactcgcccgagtactcgccctcccagtcgcccacgcgctctcgcagaccaagctaaactcaacaacgacaacatcgccaatgctattaagagtctcaccggtcacagatacgttgactctcaggtacttcaacttccctaacctcctaatctcatgcttcaacacgaagagtttgattttcttcaacagggatttcaacttgttcatgttcaatttctggagacgggggaatggaatgagattgacagccaactttttcaacactgagcttcctggattgtggtttcaggtctgccattcgcttggtggaggaaccggacctggtatggggactctgttgatttcgaaaatcagggaggaataccctgacaggatgatgctcactttctctgtgtttccatcaccaaaagtctccgatacggtggttgagccctataatgctaccttgtctgtgcaccagcttgttgagaatgctgatgagtgtatggttcttgataacgaagctctctatgacattttcttccgcacactcaagctcagtaccccaagctgtaagatcctattctga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04145 ko04540 ko05130 map04145 map04540 map05130
Pfam Domains
Protein Families
Tubulin Tubulin_C

Protein Analysis

272

Amino Acids

31.2

Weight (kDa)

9.32

Isoelectric Point (pI)

69.57

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 287
AciI CCGC 3 cut(s) 190, 193, 775
AclWI GGATC 1 cut(s) 802
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 1 cut(s) 162
AcyI GRCGYC 2 cut(s) 97, 187
AfaI GTAC 3 cut(s) 264, 377, 793
AfiI CCNNNNNNNGG 1 cut(s) 578
AgeI ACCGGT 1 cut(s) 349
AgsI TTSAA 6 cut(s) 382, 412, 436, 448, 463, 511
AjnI CCWGG 2 cut(s) 523, 571
AluBI AGCT 8 cut(s) 11, 72, 304, 520, 712, 755, 787, 801
AluI AGCT 8 cut(s) 11, 72, 304, 520, 712, 755, 787, 801
Alw21I GWGCWC 1 cut(s) 708
Alw26I GTCTC 4 cut(s) 247, 349, 466, 667
Alw44I GTGCAC 1 cut(s) 704
AlwI GGATC 1 cut(s) 802
Ama87I CYCGRG 1 cut(s) 258
AoxI GGCC 1 cut(s) 3
ApaLI GTGCAC 1 cut(s) 704
ApoI RAATTY 1 cut(s) 162
AsiGI ACCGGT 1 cut(s) 349
AspLEI GCGC 1 cut(s) 289
AspS9I GGNCC 2 cut(s) 215, 569
AsuC2I CCSGG 1 cut(s) 213
AsuHPI GGTGA 6 cut(s) 98, 116, 191, 197, 339, 645
AsuII TTCGAA 1 cut(s) 596
AvaI CYCGRG 1 cut(s) 258
AvaII GGWCC 2 cut(s) 215, 569
BaeGI GKGCMC 1 cut(s) 708
BaeI ACNNNNGTAYC 2 cut(s) 660, 693
BanII GRGCYC 2 cut(s) 87, 684
BarI GAAGNNNNNNTAC 2 cut(s) 368, 400
Bbv12I GWGCWC 1 cut(s) 708
BccI CCATC 1 cut(s) 658
BceAI ACGGC 2 cut(s) 180, 224
BciT130I CCWGG 2 cut(s) 525, 573
BcnI CCSGG 1 cut(s) 213
BcoDI GTCTC 4 cut(s) 247, 349, 466, 667
BisI GCNGC 2 cut(s) 190, 193
BlsI GCNGC 2 cut(s) 191, 194
BmcAI AGTACT 1 cut(s) 264
Bme1390I CCNGG 3 cut(s) 213, 525, 573
Bme18I GGWCC 2 cut(s) 215, 569
BmeT110I CYCGRG 1 cut(s) 258
BmgT120I GGNCC 2 cut(s) 215, 569
BmiI GGNNCC 1 cut(s) 565
BmrFI CCNGG 3 cut(s) 213, 525, 573
BmrI ACTGGG 1 cut(s) 269
BmsI GCATC 1 cut(s) 618
BmuI ACTGGG 1 cut(s) 269
BplI GAGNNNNNCTC 2 cut(s) 111, 143
BpmI CTGGAG 1 cut(s) 490
Bpu14I TTCGAA 1 cut(s) 596
BpuEI CTTGAG 2 cut(s) 58, 767
BpuMI CCSGG 1 cut(s) 213
BsaHI GRCGYC 2 cut(s) 97, 187
BsaWI WCCGGW 2 cut(s) 349, 566
BsaXI ACNNNNNCTCC 2 cut(s) 255, 285
Bsc4I CCNNNNNNNGG 1 cut(s) 578
Bse118I RCCGGY 1 cut(s) 349
Bse1I ACTGG 1 cut(s) 275
BseBI CCWGG 2 cut(s) 525, 573
BseGI GGATG 1 cut(s) 630
BseLI CCNNNNNNNGG 1 cut(s) 578
BseMII CTCAG 3 cut(s) 385, 507, 802
BseNI ACTGG 1 cut(s) 275
BseSI GKGCMC 1 cut(s) 708
Bsh1236I CGCG 1 cut(s) 287
BshFI GGCC 1 cut(s) 5
BshTI ACCGGT 1 cut(s) 349
BsiHKAI GWGCWC 1 cut(s) 708
BsiHKCI CYCGRG 1 cut(s) 258
BsiSI CCGG 3 cut(s) 213, 350, 567
BslFI GGGAC 1 cut(s) 595
BslI CCNNNNNNNGG 1 cut(s) 578
BsmAI GTCTC 4 cut(s) 247, 349, 466, 667
BsmBI CGTCTC 2 cut(s) 247, 466
BsmFI GGGAC 1 cut(s) 595
BsmI GAATGC 1 cut(s) 727
BsnI GGCC 1 cut(s) 5
BsoBI CYCGRG 1 cut(s) 258
Bsp119I TTCGAA 1 cut(s) 596
Bsp1286I GDGCHC 3 cut(s) 87, 684, 708
Bsp143I GATC 2 cut(s) 121, 807
BspACI CCGC 3 cut(s) 190, 193, 775
BspANI GGCC 1 cut(s) 5
BspCNI CTCAG 3 cut(s) 384, 508, 801
BspFNI CGCG 1 cut(s) 287
BspLI GGNNCC 1 cut(s) 565
BspPI GGATC 1 cut(s) 802
BspT104I TTCGAA 1 cut(s) 596
BsrFI RCCGGY 1 cut(s) 349
BsrI ACTGG 1 cut(s) 275
BssAI RCCGGY 1 cut(s) 349
BssMI GATC 2 cut(s) 121, 807
BssNI GRCGYC 2 cut(s) 97, 187
Bst2UI CCWGG 2 cut(s) 525, 573
Bst4CI ACNGT 1 cut(s) 673
Bst6I CTCTTC 3 cut(s) 42, 156, 413
BstACI GRCGYC 2 cut(s) 97, 187
BstBI TTCGAA 1 cut(s) 596
BstDEI CTNAG 3 cut(s) 371, 516, 788
BstF5I GGATG 1 cut(s) 630
BstFNI CGCG 1 cut(s) 287
BstHHI GCGC 1 cut(s) 289
BstKTI GATC 2 cut(s) 124, 810
BstMAI GTCTC 4 cut(s) 247, 349, 466, 667
BstMBI GATC 2 cut(s) 121, 807
BstMWI GCNNNNNNNGC 1 cut(s) 301
BstNI CCWGG 2 cut(s) 525, 573
BstSCI CCNGG 3 cut(s) 211, 523, 571
BstSLI GKGCMC 1 cut(s) 708
BstUI CGCG 1 cut(s) 287
BstX2I RGATCY 1 cut(s) 807
BstXI CCANNNNNNTGG 1 cut(s) 554
BstYI RGATCY 1 cut(s) 807
BsuRI GGCC 1 cut(s) 5
BtgZI GCGATG 1 cut(s) 309
BtsCI GGATG 1 cut(s) 630
BtsIMutI CAGTG 1 cut(s) 513
CfoI GCGC 1 cut(s) 289
Cfr10I RCCGGY 1 cut(s) 349
Cfr13I GGNCC 2 cut(s) 215, 569
CseI GACGC 2 cut(s) 105, 195
CsiI ACCWGGT 1 cut(s) 571
Csp6I GTAC 3 cut(s) 263, 376, 792
CspAI ACCGGT 1 cut(s) 349
CviAII CATG 2 cut(s) 405, 457
CviQI GTAC 3 cut(s) 263, 376, 792
DdeI CTNAG 3 cut(s) 371, 516, 788
DpnI GATC 2 cut(s) 123, 809
DpnII GATC 2 cut(s) 121, 807
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 3 cut(s) 42, 156, 413
EarI CTCTTC 3 cut(s) 42, 156, 413
Eco24I GRGCYC 2 cut(s) 87, 684
Eco47I GGWCC 2 cut(s) 215, 569
Eco88I CYCGRG 1 cut(s) 258
EcoRI GAATTC 1 cut(s) 162
EcoRII CCWGG 2 cut(s) 523, 571
EcoT38I GRGCYC 2 cut(s) 87, 684
Esp3I CGTCTC 2 cut(s) 247, 466
FaeI CATG 2 cut(s) 408, 460
FaiI YATR 7 cut(s) 60, 406, 458, 578, 687, 737, 762
FaqI GGGAC 1 cut(s) 595
FatI CATG 2 cut(s) 404, 456
Fnu4HI GCNGC 2 cut(s) 190, 193
FokI GGATG 1 cut(s) 637
FriOI GRGCYC 2 cut(s) 87, 684
Fsp4HI GCNGC 2 cut(s) 190, 193
GlaI GCGC 1 cut(s) 288
GluI GCNGC 2 cut(s) 190, 193
GsuI CTGGAG 1 cut(s) 490
HaeIII GGCC 1 cut(s) 5
HapII CCGG 3 cut(s) 213, 350, 567
HgaI GACGC 2 cut(s) 105, 195
HhaI GCGC 1 cut(s) 289
Hin1I GRCGYC 2 cut(s) 97, 187
Hin1II CATG 2 cut(s) 408, 460
Hin6I GCGC 1 cut(s) 287
HinP1I GCGC 1 cut(s) 287
HincII GTYRAC 1 cut(s) 366
HindII GTYRAC 1 cut(s) 366
HinfI GANTC 5 cut(s) 17, 78, 342, 367, 583
HpaII CCGG 3 cut(s) 213, 350, 567
HphI GGTGA 6 cut(s) 98, 116, 191, 197, 339, 645
Hpy166II GTNNAC 2 cut(s) 366, 706
Hpy188I TCNGA 3 cut(s) 22, 667, 818
Hpy188III TCNNGA 4 cut(s) 14, 75, 469, 743
Hpy8I GTNNAC 2 cut(s) 366, 706
Hpy99I CGWCG 3 cut(s) 99, 186, 189
HpyAV CCTTC 2 cut(s) 122, 212
HpyCH4III ACNGT 1 cut(s) 673
HpyCH4IV ACGT 1 cut(s) 362
HpyCH4V TGCA 1 cut(s) 706
HpyF10VI GCNNNNNNNGC 1 cut(s) 301
HpyF3I CTNAG 3 cut(s) 371, 516, 788
HpySE526I ACGT 1 cut(s) 362
Hsp92I GRCGYC 2 cut(s) 97, 187
Hsp92II CATG 2 cut(s) 408, 460
HspAI GCGC 1 cut(s) 287
Kzo9I GATC 2 cut(s) 121, 807
LweI GCATC 1 cut(s) 618
MabI ACCWGGT 1 cut(s) 571
MaeII ACGT 1 cut(s) 362
MaeIII GTNAC 3 cut(s) 197, 247, 352
MalI GATC 2 cut(s) 123, 809
MboI GATC 2 cut(s) 121, 807
MflI RGATCY 1 cut(s) 807
MhlI GDGCHC 3 cut(s) 87, 684, 708
MluCI AATT 3 cut(s) 162, 170, 463
MlyI GAGTC 3 cut(s) 351, 361, 577
MmeI TCCRAC 1 cut(s) 45
MseI TTAA 1 cut(s) 338
MspI CCGG 3 cut(s) 213, 350, 567
MspR9I CCNGG 3 cut(s) 213, 525, 573
Mva1269I GAATGC 1 cut(s) 727
MvaI CCWGG 2 cut(s) 525, 573
MvnI CGCG 1 cut(s) 287
MwoI GCNNNNNNNGC 1 cut(s) 301
NciI CCSGG 1 cut(s) 213
NdeII GATC 2 cut(s) 121, 807
NlaIII CATG 2 cut(s) 408, 460
NlaIV GGNNCC 1 cut(s) 565
NmuCI GTSAC 3 cut(s) 197, 247, 352
NspV TTCGAA 1 cut(s) 596
PcsI WCGNNNNNNNCGW 4 cut(s) 181, 193, 237, 243
PctI GAATGC 1 cut(s) 727
PfeI GAWTC 2 cut(s) 17, 78
PfoI TCCNGGA 1 cut(s) 523
PinAI ACCGGT 1 cut(s) 349
PkrI GCNGC 2 cut(s) 191, 194
PleI GAGTC 3 cut(s) 350, 361, 577
PpsI GAGTC 3 cut(s) 350, 361, 577
Psp6I CCWGG 2 cut(s) 523, 571
PspGI CCWGG 2 cut(s) 523, 571
PspN4I GGNNCC 1 cut(s) 565
PspPI GGNCC 2 cut(s) 215, 569
PsuI RGATCY 1 cut(s) 807
RsaI GTAC 3 cut(s) 264, 377, 793
RsaNI GTAC 3 cut(s) 263, 376, 792
SaqAI TTAA 1 cut(s) 338
SatI GCNGC 2 cut(s) 190, 193
Sau3AI GATC 2 cut(s) 121, 807
Sau96I GGNCC 2 cut(s) 215, 569
ScaI AGTACT 1 cut(s) 264
SchI GAGTC 3 cut(s) 351, 361, 577
ScrFI CCNGG 3 cut(s) 213, 525, 573
SduI GDGCHC 3 cut(s) 87, 684, 708
SexAI ACCWGGT 1 cut(s) 571
SfaNI GCATC 1 cut(s) 618
SfuI TTCGAA 1 cut(s) 596
SinI GGWCC 2 cut(s) 215, 569
SmlI CTYRAG 3 cut(s) 12, 73, 782
SmoI CTYRAG 3 cut(s) 12, 73, 782
Sse9I AATT 3 cut(s) 162, 170, 463
SsiI CCGC 3 cut(s) 190, 193, 775
StyD4I CCNGG 3 cut(s) 211, 523, 571
TaaI ACNGT 1 cut(s) 673
TaiI ACGT 1 cut(s) 365
TaqI TCGA 4 cut(s) 81, 160, 181, 596
TasI AATT 3 cut(s) 162, 170, 463
TatI WGTACW 1 cut(s) 262
TauI GCSGC 2 cut(s) 192, 195
TfiI GAWTC 2 cut(s) 17, 78
Tru1I TTAA 1 cut(s) 338
Tru9I TTAA 1 cut(s) 338
TscAI CASTG 1 cut(s) 520
TseFI GTSAC 3 cut(s) 197, 247, 352
Tsp45I GTSAC 3 cut(s) 197, 247, 352
TspDTI ATGAA 1 cut(s) 445
TspGWI ACGGA 1 cut(s) 235
TspRI CASTG 1 cut(s) 520
VneI GTGCAC 1 cut(s) 704
VpaK11BI GGWCC 2 cut(s) 215, 569
XapI RAATTY 1 cut(s) 162
XcmI CCANNNNNNNNNTGG 1 cut(s) 554
ZrmI AGTACT 1 cut(s) 264
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.