RLG00000031997

Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0010657
Physical Location & Seq
Forward (+)
10109747 .. 10110903
1157 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000031997

Sequence Viewer

Length: 348 bp
ATGACTTTGTTTGAGGATGGCGTGGTTTTTGTTGAGGCGGAGTGCAGCTCCGTCGTGGATGACTTTGTTGAGGCGCAGAGCAGCTCTGAAATCAACTTTGAGTTCCAGGTGTGCCATTCTTTGGGTGGTGGTACTGGATCTGGAATGGGAATGCTTCTGATTTCCAAGATTAGGGAGGAGTACCCAGATCAGATGATGATGACTTTCTCTGTGTTCCCATCTCCCAAGGTATCAGATACTGTTGTGGAGTCTTACAATGCTACTTTGTCTGTTCTTCAACTTGTGGAGAACCCATACTTGAGCTTAGCCCCAACTGTAAGTTCATTCTCAATCTTTACTTGGATGTAA

Protein Analysis

116

Amino Acids

12.63

Weight (kDa)

4.05

Isoelectric Point (pI)

43.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Tubulin PF00091 33 - 97 4.7e-17 Tubulin/FtsZ family, GTPase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 121
AciI CCGC 1 cut(s) 38
AclWI GGATC 1 cut(s) 145
AfaI GTAC 2 cut(s) 133, 182
AfiI CCNNNNNNNGG 2 cut(s) 121, 171
AgsI TTSAA 1 cut(s) 278
AjnI CCWGG 1 cut(s) 105
AjuI GAANNNNNNNTTGG 2 cut(s) 304, 336
AluBI AGCT 3 cut(s) 48, 84, 303
AluI AGCT 3 cut(s) 48, 84, 303
AlwI GGATC 1 cut(s) 145
AlwNI CAGNNNCTG 1 cut(s) 239
ApeKI GCWGC 2 cut(s) 45, 81
AspLEI GCGC 1 cut(s) 76
BaeI ACNNNNGTAYC 2 cut(s) 228, 261
BbvI GCAGC 2 cut(s) 57, 93
BccI CCATC 2 cut(s) 11, 226
BcgI CGANNNNNNTGC 2 cut(s) 34, 68
BciT130I CCWGG 1 cut(s) 107
BisI GCNGC 2 cut(s) 46, 82
BlpI GCTNAGC 1 cut(s) 304
BlsI GCNGC 2 cut(s) 47, 83
Bme1390I CCNGG 1 cut(s) 107
BmrFI CCNGG 1 cut(s) 107
BplI GAGNNNNNCTC 2 cut(s) 32, 64
Bpu1102I GCTNAGC 1 cut(s) 304
BpuEI CTTGAG 1 cut(s) 319
BsaJI CCNNGG 1 cut(s) 225
Bsc4I CCNNNNNNNGG 2 cut(s) 121, 171
Bse1I ACTGG 1 cut(s) 139
BseBI CCWGG 1 cut(s) 107
BseDI CCNNGG 1 cut(s) 225
BseGI GGATG 3 cut(s) 22, 64, 348
BseLI CCNNNNNNNGG 2 cut(s) 121, 171
BseNI ACTGG 1 cut(s) 139
BseRI GAGGAG 1 cut(s) 191
BseXI GCAGC 2 cut(s) 57, 93
BsgI GTGCAG 1 cut(s) 64
BslI CCNNNNNNNGG 2 cut(s) 121, 171
BsmI GAATGC 1 cut(s) 156
Bsp143I GATC 2 cut(s) 137, 187
Bsp1720I GCTNAGC 1 cut(s) 304
BspACI CCGC 1 cut(s) 38
BspPI GGATC 1 cut(s) 145
BsrI ACTGG 1 cut(s) 139
BssECI CCNNGG 1 cut(s) 225
BssMI GATC 2 cut(s) 137, 187
BssT1I CCWWGG 1 cut(s) 225
Bst2UI CCWGG 1 cut(s) 107
Bst4CI ACNGT 2 cut(s) 241, 316
BstDEI CTNAG 1 cut(s) 304
BstF5I GGATG 3 cut(s) 22, 64, 348
BstHHI GCGC 1 cut(s) 76
BstKTI GATC 2 cut(s) 140, 190
BstMBI GATC 2 cut(s) 137, 187
BstNI CCWGG 1 cut(s) 107
BstSCI CCNGG 1 cut(s) 105
BstV1I GCAGC 2 cut(s) 57, 93
BstX2I RGATCY 1 cut(s) 137
BstYI RGATCY 1 cut(s) 137
BtsCI GGATG 3 cut(s) 22, 64, 348
CaiI CAGNNNCTG 1 cut(s) 239
CfoI GCGC 1 cut(s) 76
Csp6I GTAC 2 cut(s) 132, 181
CviJI RGCY 4 cut(s) 48, 84, 303, 308
CviKI_1 RGCY 4 cut(s) 48, 84, 303, 308
CviQI GTAC 2 cut(s) 132, 181
DdeI CTNAG 1 cut(s) 304
DpnI GATC 2 cut(s) 139, 189
DpnII GATC 2 cut(s) 137, 187
EciI GGCGGA 1 cut(s) 53
Eco130I CCWWGG 1 cut(s) 225
EcoRII CCWGG 1 cut(s) 105
EcoT14I CCWWGG 1 cut(s) 225
ErhI CCWWGG 1 cut(s) 225
FaiI YATR 1 cut(s) 295
Fnu4HI GCNGC 2 cut(s) 46, 82
FokI GGATG 2 cut(s) 29, 71
Fsp4HI GCNGC 2 cut(s) 46, 82
GlaI GCGC 1 cut(s) 75
GluI GCNGC 2 cut(s) 46, 82
HhaI GCGC 1 cut(s) 76
Hin6I GCGC 1 cut(s) 74
HinP1I GCGC 1 cut(s) 74
HinfI GANTC 1 cut(s) 248
Hpy188I TCNGA 4 cut(s) 88, 159, 192, 235
Hpy188III TCNNGA 1 cut(s) 141
Hpy99I CGWCG 1 cut(s) 56
HpyCH4III ACNGT 2 cut(s) 241, 316
HpyCH4V TGCA 1 cut(s) 45
HpyF3I CTNAG 1 cut(s) 304
HspAI GCGC 1 cut(s) 74
Kzo9I GATC 2 cut(s) 137, 187
LmnI GCTCC 1 cut(s) 53
LpnPI CCDG 5 cut(s) 92, 119, 120, 126, 198
Lsp1109I GCAGC 2 cut(s) 57, 93
MalI GATC 2 cut(s) 139, 189
MboI GATC 2 cut(s) 137, 187
MboII GAAGA 1 cut(s) 266
MflI RGATCY 1 cut(s) 137
MlyI GAGTC 1 cut(s) 257
MnlI CCTC 4 cut(s) 7, 28, 64, 169
MspR9I CCNGG 1 cut(s) 107
Mva1269I GAATGC 1 cut(s) 156
MvaI CCWGG 1 cut(s) 107
NdeII GATC 2 cut(s) 137, 187
PctI GAATGC 1 cut(s) 156
PflMI CCANNNNNTGG 1 cut(s) 121
PkrI GCNGC 2 cut(s) 47, 83
PleI GAGTC 1 cut(s) 256
PpsI GAGTC 1 cut(s) 256
Psp6I CCWGG 1 cut(s) 105
PspGI CCWGG 1 cut(s) 105
PstNI CAGNNNCTG 1 cut(s) 239
PsuI RGATCY 1 cut(s) 137
RsaI GTAC 2 cut(s) 133, 182
RsaNI GTAC 2 cut(s) 132, 181
SatI GCNGC 2 cut(s) 46, 82
Sau3AI GATC 2 cut(s) 137, 187
SchI GAGTC 1 cut(s) 257
ScrFI CCNGG 1 cut(s) 107
SetI ASST 5 cut(s) 50, 86, 111, 231, 305
SmlI CTYRAG 1 cut(s) 298
SmoI CTYRAG 1 cut(s) 298
SsiI CCGC 1 cut(s) 38
StyD4I CCNGG 1 cut(s) 105
StyI CCWWGG 1 cut(s) 225
TaaI ACNGT 2 cut(s) 241, 316
TseI GCWGC 2 cut(s) 45, 81
TspDTI ATGAA 1 cut(s) 312
TspGWI ACGGA 1 cut(s) 40
Van91I CCANNNNNTGG 1 cut(s) 121
XcmI CCANNNNNNNNNTGG 1 cut(s) 122
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.