MD05G1275200.v1.1

Belongs to the NPH3 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0014576
Physical Location & Seq
Reverse (-)
40957961 .. 40962209
4249 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1275200.v1.1.491

Sequence Viewer

Length: 1905 bp
ATGGCTTGCGTGAAATTGGGATCTAAAACTGATGCATTCCATAAACAAGGACAGGCCTGGTTCTGCACCACTGGGCTTCCTAGTGACATTGTTGTTGAAGTTGGAGAGATGTCATTTCATCTGCATAAGTTTCCTTTGCTCTCCAAAAGTGGGGTTATGGAAAGACTGATTTCCGAAGCATCTGAAGGAGAAGAAGGACGTGTCATAACCATCCCAGATATTCCTGGTGGGGCTAAAACATTTGAATTGGTAGCCAAGTTCTGCTACGGGGTGAAATTTGAGCTAACTGCCTCAAATGTTGTATATCTACGATGTGCTGCTGAGCATCTAGAAATGACTGAAGAGTACGGGGAGGACAACTTAATCACACAAAGTGAAGCCTTTCTCAATCAAGTAGTTCTTCGAAATTGGAAGGACTCTCTAAAGGCACTTCAAACTTGTGATGACATTCTCCCTTATGCTGAAGAACTCAACATTCCAAAAAGGTGCATGGAATCACTTGCCACAAAAGCATCCACTGATCCTAATTTGTTTGGGTGGCCTGTCAAGGAGCATGGCGGCCCCATGCAAAGTCCTGGTGGGAGCGTCCTGTGGAATGGAATAAGTACGGGGGCCAGACCAAAGAAATCAAGTTCAGATTGGTGGTACGAGGATGCATCAACCTTAAGCTTGCCTCTCTACCAGAGGCTGATTTCGGTCATGGAATCTCGTGGCATCAAACAGGAGATTATTGCAGCCTCAATCACTTTTTATGCAAAAAAGTACCTTCCTGGGCTCAATCGGCGTCAAGGCAATGATTATAGTACTAATCTTAAACCAGTGTCCTTGGGGTCCCCACCATCAGAAGAAGACCAGAAGCTCTTACTTGAAGAGGTCGATCGGTTACTTCCATTTCAGAAGCGTGTAGTTCCAACTAAATTTTTGTTTGGTCTACTTCGAACAGCCATGATTCTTCGAGCGAACCCTTCTTGCACATCAAATTTTGAGAAAAGAATTGGCATGCAGCTTGATCAAGCTACGTTGGAAGATCTTTTGATGCCAAACTTCTCTTATTCTATGGAGACTCTATACAATGTTGACTGCGTGCAACGAATTCTAGACCATTTCCTTGCCATGGAACAAATTACAGGAGGAGTGTCTCCATGTTCGGTCGATGATGAACAATTGATCGGGTCTTCAGGCCCTTCACTGACCCCAATCACAATGGTAGCCAAGCTCATTGATGGATACCTTGCAGAGGTCGCCCCTGATGTCAACTTGAAGCTCCCTAAGTTTCAGGCTCTTGCTGCTGCTGTTCCAGAATATGCCAGACCCTTGGATGATGGTCTTTACCGCGCAATAGATATTTTTTTGAAGTCACACCCATGGTTGGCAGAGTCCGACAGGGAACAGCTCTGCCGGTTGATGGACTGCCAGAAGCTTTCCTTGGAAGCTTGCACTCATGCTGCACAGAACGAGCGGCTACCCCTTAGAATAGTTGTTCAAGTTCTCTTCTTTGAGCAGCTTCAGCTTAGGACATCTATTGCGGACTGCTTTCTGGTTTCAGAAAATCTTGATGGATCAAGACAGTTAAGAAGCGGGTTTGCTGGGTCTACCGAGGGAGGCTGGGCCACAACCGTGAGGGAAAATCAGGTTTTAAAGGTTGGGATGGATAATATGAGGATGCGGGTATCTGAGCTTGAGAAGGAGTGTTCGAACATGAGACAAGAGATCGAGAAGTTGGGTCGCGTAAAAGGTTCTAGCACGTGGGGAAACGTATCAAAGAAATTCGGGTTCAAGATTAAGTCCCAGATGTGCAGCGCTCAAGAGGGATCGGTCACCAGTCAGCATAATGCGAGTGAGCAAGTCGAGAAACTAAAGGAGAGGCATGGAAAGCAAAAGAAAGGCTCTGTTAAAGATGAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
BTB NPH3

Protein Analysis

635

Amino Acids

70.73

Weight (kDa)

5.86

Isoelectric Point (pI)

43.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BTB PF00651 26 - 114 5.3e-08 BTB/POZ domain
NPH3 PF03000 213 - 479 1.3e-96 NPH3 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014576)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G30440
fragaria_vesca FvH4_3g09890 FvH4_3g09890
malus_domestica MD05G1275200.v1.1 MD10G1254600.v1.1
prunus_persica Prupe.4G088800_v2.0.a1
pyrus_communis pycom05g25650 pycom10g21020
rosa_chinensis RchiOBHm_Chr5g0015601
rosa_laevigata RLG00000023153 RLG00000032212
rosa_multiflora Rmu_sc0003230.1_g000001
rosa_roxburghii Rroxscaffold_1G00060870
rosa_rugosa Rorug05G0028800 Rorug05G0028800
rosa_samantha Rh5AG122100
rosa_wichuraiana Rw5G010530

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 1459
AccI GTMKAC 2 cut(s) 931, 1592
AccII CGCG 2 cut(s) 1335, 1728
AciI CCGC 6 cut(s) 558, 1333, 1459, 1526, 1578, 1666
AclWI GGATC 4 cut(s) 28, 515, 1567, 1819
AcsI RAATTY 5 cut(s) 275, 917, 979, 1092, 1766
AcuI CTGAAG 5 cut(s) 204, 360, 483, 1161, 1490
AcvI CACGTG 1 cut(s) 1746
AcyI GRCGYC 1 cut(s) 784
AdeI CACNNNGTG 1 cut(s) 374
AfaI GTAC 5 cut(s) 347, 607, 647, 764, 805
AfeI AGCGCT 1 cut(s) 1801
AfiI CCNNNNNNNGG 5 cut(s) 150, 1114, 1237, 1369, 1405
AflII CTTAAG 1 cut(s) 664
AflIII ACRYGT 1 cut(s) 199
AgsI TTSAA 8 cut(s) 98, 245, 434, 869, 1261, 1354, 1484, 1777
AjiI CACGTC 1 cut(s) 200
AjnI CCWGG 4 cut(s) 56, 223, 574, 769
AjuI GAANNNNNNNTTGG 2 cut(s) 1409, 1441
AleI CACNNNNGTG 1 cut(s) 1616
Alw26I GTCTC 3 cut(s) 1055, 1143, 1696
AlwI GGATC 4 cut(s) 28, 515, 1567, 1819
AlwNI CAGNNNCTG 1 cut(s) 688
Aor51HI AGCGCT 1 cut(s) 1801
AoxI GGCC 6 cut(s) 54, 539, 559, 612, 1180, 1608
ApeKI GCWGC 8 cut(s) 317, 734, 1003, 1286, 1289, 1445, 1501, 1797
ApoI RAATTY 5 cut(s) 275, 917, 979, 1092, 1766
Asp700I GAANNNNTTC 1 cut(s) 381
AspLEI GCGC 2 cut(s) 1337, 1802
AspS9I GGNCC 5 cut(s) 560, 612, 831, 1181, 1608
AsuHPI GGTGA 2 cut(s) 283, 1810
AsuII TTCGAA 3 cut(s) 403, 937, 1694
AvaII GGWCC 1 cut(s) 831
BanII GRGCYC 1 cut(s) 777
BauI CACGAG 1 cut(s) 708
BbrPI CACGTG 1 cut(s) 1746
BbsI GAAGAC 2 cut(s) 855, 1167
BbvI GCAGC 8 cut(s) 304, 746, 1015, 1273, 1276, 1432, 1513, 1809
BccI CCATC 7 cut(s) 218, 847, 1217, 1316, 1399, 1550, 1642
BciT130I CCWGG 4 cut(s) 58, 225, 576, 771
BciVI GTATCC 1 cut(s) 1220
BclI TGATCA 1 cut(s) 1009
BcoDI GTCTC 3 cut(s) 1055, 1143, 1696
BfaI CTAG 4 cut(s) 81, 329, 1097, 1740
BfoI RGCGCY 1 cut(s) 1803
BfrI CTTAAG 1 cut(s) 664
BfuI GTATCC 1 cut(s) 1220
BglII AGATCT 1 cut(s) 1027
BlpI GCTNAGC 1 cut(s) 321
BmcAI AGTACT 1 cut(s) 805
Bme1390I CCNGG 4 cut(s) 58, 225, 576, 771
Bme18I GGWCC 1 cut(s) 831
BmgBI CACGTC 1 cut(s) 200
BmgT120I GGNCC 5 cut(s) 560, 612, 831, 1181, 1608
BmiI GGNNCC 4 cut(s) 562, 613, 832, 833
BmrFI CCNGG 4 cut(s) 58, 225, 576, 771
BmrI ACTGGG 1 cut(s) 81
BmsI GCATC 9 cut(s) 22, 188, 334, 521, 643, 665, 723, 1026, 1653
BmuI ACTGGG 1 cut(s) 81
BpiI GAAGAC 2 cut(s) 855, 1167
Bpu10I CCTNAGC 1 cut(s) 1511
Bpu1102I GCTNAGC 1 cut(s) 321
Bpu14I TTCGAA 3 cut(s) 403, 937, 1694
BpuEI CTTGAG 2 cut(s) 1700, 1788
BsaAI YACGTR 1 cut(s) 1746
BsaHI GRCGYC 1 cut(s) 784
BsaJI CCNNGG 7 cut(s) 770, 825, 1113, 1314, 1364, 1425, 1596
Bsc4I CCNNNNNNNGG 5 cut(s) 150, 1114, 1237, 1369, 1405
Bse118I RCCGGY 1 cut(s) 1398
Bse1I ACTGG 3 cut(s) 76, 818, 1821
Bse3DI GCAATG 1 cut(s) 799
BseBI CCWGG 4 cut(s) 58, 225, 576, 771
BseDI CCNNGG 7 cut(s) 770, 825, 1113, 1314, 1364, 1425, 1596
BseGI GGATG 6 cut(s) 210, 512, 658, 1324, 1653, 1668
BseLI CCNNNNNNNGG 5 cut(s) 150, 1114, 1237, 1369, 1405
BseMI GCAATG 1 cut(s) 799
BseMII CTCAG 2 cut(s) 312, 1665
BseNI ACTGG 3 cut(s) 76, 818, 1821
BseRI GAGGAG 1 cut(s) 1146
BseXI GCAGC 8 cut(s) 304, 746, 1015, 1273, 1276, 1432, 1513, 1809
BseYI CCCAGC 2 cut(s) 1586, 1605
BsgI GTGCAG 3 cut(s) 49, 1431, 1816
Bsh1236I CGCG 2 cut(s) 1335, 1728
Bsh1285I CGRYCG 2 cut(s) 880, 1152
BshFI GGCC 6 cut(s) 56, 541, 561, 614, 1182, 1610
BsiEI CGRYCG 2 cut(s) 880, 1152
BsiSI CCGG 1 cut(s) 1399
BslFI GGGAC 2 cut(s) 817, 1771
BslI CCNNNNNNNGG 5 cut(s) 150, 1114, 1237, 1369, 1405
BsmAI GTCTC 3 cut(s) 1055, 1143, 1696
BsmFI GGGAC 2 cut(s) 817, 1771
BsmI GAATGC 1 cut(s) 35
BsnI GGCC 6 cut(s) 56, 541, 561, 614, 1182, 1610
Bsp119I TTCGAA 3 cut(s) 403, 937, 1694
Bsp1286I GDGCHC 1 cut(s) 777
Bsp143I GATC 9 cut(s) 20, 520, 877, 1009, 1027, 1167, 1559, 1710, 1811
Bsp1720I GCTNAGC 1 cut(s) 321
Bsp19I CCATGG 2 cut(s) 1113, 1364
BspACI CCGC 6 cut(s) 558, 1333, 1459, 1526, 1578, 1666
BspANI GGCC 6 cut(s) 56, 541, 561, 614, 1182, 1610
BspCNI CTCAG 2 cut(s) 313, 1666
BspFNI CGCG 2 cut(s) 1335, 1728
BspLI GGNNCC 4 cut(s) 562, 613, 832, 833
BspPI GGATC 4 cut(s) 28, 515, 1567, 1819
BspT104I TTCGAA 3 cut(s) 403, 937, 1694
BspTI CTTAAG 1 cut(s) 664
BsrBI CCGCTC 1 cut(s) 1459
BsrDI GCAATG 1 cut(s) 799
BsrFI RCCGGY 1 cut(s) 1398
BsrI ACTGG 3 cut(s) 76, 818, 1821
BssAI RCCGGY 1 cut(s) 1398
BssECI CCNNGG 7 cut(s) 770, 825, 1113, 1314, 1364, 1425, 1596
BssMI GATC 9 cut(s) 20, 520, 877, 1009, 1027, 1167, 1559, 1710, 1811
BssNI GRCGYC 1 cut(s) 784
BssSI CACGAG 1 cut(s) 708
BssT1I CCWWGG 5 cut(s) 825, 1113, 1314, 1364, 1425
Bst2BI CACGAG 1 cut(s) 708
Bst2UI CCWGG 4 cut(s) 58, 225, 576, 771
Bst4CI ACNGT 2 cut(s) 1569, 1618
Bst6I CTCTTC 3 cut(s) 336, 864, 1496
BstACI GRCGYC 1 cut(s) 784
BstAFI CTTAAG 1 cut(s) 664
BstBAI YACGTR 1 cut(s) 1746
BstBI TTCGAA 3 cut(s) 403, 937, 1694
BstC8I GCNNGC 5 cut(s) 7, 671, 1001, 1085, 1435
BstDEI CTNAG 5 cut(s) 321, 1269, 1469, 1511, 1674
BstDSI CCRYGG 2 cut(s) 1113, 1364
BstEII GGTNACC 1 cut(s) 1816
BstENI CCTNNNNNAGG 1 cut(s) 1235
BstF5I GGATG 6 cut(s) 210, 512, 658, 1324, 1653, 1668
BstFNI CGCG 2 cut(s) 1335, 1728
BstH2I RGCGCY 1 cut(s) 1803
BstHHI GCGC 2 cut(s) 1337, 1802
BstKTI GATC 9 cut(s) 23, 523, 880, 1012, 1030, 1170, 1562, 1713, 1814
BstMAI GTCTC 3 cut(s) 1055, 1143, 1696
BstMBI GATC 9 cut(s) 20, 520, 877, 1009, 1027, 1167, 1559, 1710, 1811
BstMCI CGRYCG 2 cut(s) 880, 1152
BstMWI GCNNNNNNNGC 6 cut(s) 509, 781, 1241, 1286, 1507, 1873
BstNI CCWGG 4 cut(s) 58, 225, 576, 771
BstNSI RCATGY 1 cut(s) 1003
BstPI GGTNACC 1 cut(s) 1816
BstSCI CCNGG 4 cut(s) 56, 223, 574, 769
BstUI CGCG 2 cut(s) 1335, 1728
BstV1I GCAGC 8 cut(s) 304, 746, 1015, 1273, 1276, 1432, 1513, 1809
BstV2I GAAGAC 2 cut(s) 855, 1167
BstX2I RGATCY 2 cut(s) 20, 1027
BstXI CCANNNNNNTGG 1 cut(s) 1315
BstYI RGATCY 2 cut(s) 20, 1027
BsuI GTATCC 1 cut(s) 1220
BsuRI GGCC 6 cut(s) 56, 541, 561, 614, 1182, 1610
BtgI CCRYGG 2 cut(s) 1113, 1364
BtrI CACGTC 1 cut(s) 200
BtsCI GGATG 6 cut(s) 210, 512, 658, 1324, 1653, 1668
BtsIMutI CAGTG 4 cut(s) 69, 516, 825, 1187
Cac8I GCNNGC 5 cut(s) 7, 671, 1001, 1085, 1435
CaiI CAGNNNCTG 1 cut(s) 688
CfoI GCGC 2 cut(s) 1337, 1802
Cfr10I RCCGGY 1 cut(s) 1398
Cfr13I GGNCC 5 cut(s) 560, 612, 831, 1181, 1608
CseI GACGC 2 cut(s) 574, 773
Csp6I GTAC 5 cut(s) 346, 606, 646, 763, 804
CviQI GTAC 5 cut(s) 346, 606, 646, 763, 804
DdeI CTNAG 5 cut(s) 321, 1269, 1469, 1511, 1674
DpnI GATC 9 cut(s) 22, 522, 879, 1011, 1029, 1169, 1561, 1712, 1813
DpnII GATC 9 cut(s) 20, 520, 877, 1009, 1027, 1167, 1559, 1710, 1811
DraI TTTAAA 1 cut(s) 1638
DraIII CACNNNGTG 1 cut(s) 374
Eam1104I CTCTTC 3 cut(s) 336, 864, 1496
EarI CTCTTC 3 cut(s) 336, 864, 1496
Eco130I CCWWGG 5 cut(s) 825, 1113, 1314, 1364, 1425
Eco147I AGGCCT 1 cut(s) 56
Eco24I GRGCYC 1 cut(s) 777
Eco47I GGWCC 1 cut(s) 831
Eco47III AGCGCT 1 cut(s) 1801
Eco57I CTGAAG 5 cut(s) 204, 360, 483, 1161, 1490
Eco72I CACGTG 1 cut(s) 1746
Eco91I GGTNACC 1 cut(s) 1816
EcoNI CCTNNNNNAGG 1 cut(s) 1235
EcoO109I RGGNCCY 2 cut(s) 831, 1181
EcoO65I GGTNACC 1 cut(s) 1816
EcoRI GAATTC 1 cut(s) 1092
EcoRII CCWGG 4 cut(s) 56, 223, 574, 769
EcoT14I CCWWGG 5 cut(s) 825, 1113, 1314, 1364, 1425
EcoT22I ATGCAT 2 cut(s) 37, 658
EcoT38I GRGCYC 1 cut(s) 777
ErhI CCWWGG 5 cut(s) 825, 1113, 1314, 1364, 1425
FalI AAGNNNNNCTT 4 cut(s) 384, 416, 1409, 1441
FaqI GGGAC 2 cut(s) 817, 1771
FauI CCCGC 2 cut(s) 1571, 1659
FbaI TGATCA 1 cut(s) 1009
FblI GTMKAC 2 cut(s) 931, 1592
FokI GGATG 6 cut(s) 197, 499, 665, 1331, 1660, 1675
FriOI GRGCYC 1 cut(s) 777
FspBI CTAG 4 cut(s) 81, 329, 1097, 1740
GlaI GCGC 2 cut(s) 1336, 1801
GsaI CCCAGC 2 cut(s) 1590, 1609
HaeII RGCGCY 1 cut(s) 1803
HaeIII GGCC 6 cut(s) 56, 541, 561, 614, 1182, 1610
HapII CCGG 1 cut(s) 1399
HgaI GACGC 2 cut(s) 574, 773
HhaI GCGC 2 cut(s) 1337, 1802
Hin1I GRCGYC 1 cut(s) 784
Hin6I GCGC 2 cut(s) 1335, 1800
HinP1I GCGC 2 cut(s) 1335, 1800
HincII GTYRAC 2 cut(s) 1078, 1255
HindII GTYRAC 2 cut(s) 1078, 1255
HindIII AAGCTT 3 cut(s) 667, 1418, 1431
HinfI GANTC 6 cut(s) 416, 494, 704, 949, 1063, 1376
HpaII CCGG 1 cut(s) 1399
HphI GGTGA 2 cut(s) 283, 1810
Hpy166II GTNNAC 4 cut(s) 932, 1078, 1255, 1593
Hpy188I TCNGA 8 cut(s) 175, 184, 637, 844, 897, 1381, 1546, 1675
Hpy188III TCNNGA 9 cut(s) 329, 1097, 1298, 1553, 1563, 1714, 1777, 1805, 1849
Hpy8I GTNNAC 4 cut(s) 932, 1078, 1255, 1593
HpyAV CCTTC 7 cut(s) 179, 188, 406, 776, 975, 1194, 1678
HpyCH4III ACNGT 2 cut(s) 1569, 1618
HpyCH4IV ACGT 4 cut(s) 199, 1019, 1745, 1755
HpyF10VI GCNNNNNNNGC 6 cut(s) 509, 781, 1241, 1286, 1507, 1873
HpyF3I CTNAG 5 cut(s) 321, 1269, 1469, 1511, 1674
HpySE526I ACGT 4 cut(s) 199, 1019, 1745, 1755
Hsp92I GRCGYC 1 cut(s) 784
HspAI GCGC 2 cut(s) 1335, 1800
KflI GGGWCCC 1 cut(s) 831
Ksp22I TGATCA 1 cut(s) 1009
Kzo9I GATC 9 cut(s) 20, 520, 877, 1009, 1027, 1167, 1559, 1710, 1811
LmnI GCTCC 3 cut(s) 550, 582, 1269
Lsp1109I GCAGC 8 cut(s) 304, 746, 1015, 1273, 1276, 1432, 1513, 1809
LweI GCATC 9 cut(s) 22, 188, 334, 521, 643, 665, 723, 1026, 1653
MaeI CTAG 4 cut(s) 81, 329, 1097, 1740
MaeII ACGT 4 cut(s) 199, 1019, 1745, 1755
MaeIII GTNAC 4 cut(s) 83, 882, 1356, 1816
MalI GATC 9 cut(s) 22, 522, 879, 1011, 1029, 1169, 1561, 1712, 1813
MbiI CCGCTC 1 cut(s) 1459
MboI GATC 9 cut(s) 20, 520, 877, 1009, 1027, 1167, 1559, 1710, 1811
MfeI CAATTG 1 cut(s) 1163
MflI RGATCY 2 cut(s) 20, 1027
MhlI GDGCHC 1 cut(s) 777
MlyI GAGTC 3 cut(s) 410, 1057, 1385
MmeI TCCRAC 4 cut(s) 82, 935, 1002, 1404
Mph1103I ATGCAT 2 cut(s) 37, 658
MroXI GAANNNNTTC 1 cut(s) 381
MseI TTAA 7 cut(s) 362, 665, 813, 1571, 1637, 1782, 1893
MslI CAYNNNNRTG 2 cut(s) 1363, 1616
MspCI CTTAAG 1 cut(s) 664
MspI CCGG 1 cut(s) 1399
MspR9I CCNGG 4 cut(s) 58, 225, 576, 771
MunI CAATTG 1 cut(s) 1163
Mva1269I GAATGC 1 cut(s) 35
MvaI CCWGG 4 cut(s) 58, 225, 576, 771
MvnI CGCG 2 cut(s) 1335, 1728
MwoI GCNNNNNNNGC 6 cut(s) 509, 781, 1241, 1286, 1507, 1873
NcoI CCATGG 2 cut(s) 1113, 1364
NdeII GATC 9 cut(s) 20, 520, 877, 1009, 1027, 1167, 1559, 1710, 1811
NlaIV GGNNCC 4 cut(s) 562, 613, 832, 833
NmuCI GTSAC 3 cut(s) 83, 1356, 1816
NsiI ATGCAT 2 cut(s) 37, 658
NspI RCATGY 1 cut(s) 1003
NspV TTCGAA 3 cut(s) 403, 937, 1694
OliI CACNNNNGTG 1 cut(s) 1616
PaeI GCATGC 1 cut(s) 1003
PceI AGGCCT 1 cut(s) 56
PctI GAATGC 1 cut(s) 35
PdmI GAANNNNTTC 1 cut(s) 381
PfeI GAWTC 3 cut(s) 494, 704, 949
Ple19I CGATCG 1 cut(s) 880
PleI GAGTC 3 cut(s) 410, 1057, 1384
PmaCI CACGTG 1 cut(s) 1746
PmlI CACGTG 1 cut(s) 1746
PpsI GAGTC 3 cut(s) 410, 1057, 1384
Ppu21I YACGTR 1 cut(s) 1746
PpuMI RGGWCCY 1 cut(s) 831
Psp5II RGGWCCY 1 cut(s) 831
Psp6I CCWGG 4 cut(s) 56, 223, 574, 769
PspCI CACGTG 1 cut(s) 1746
PspEI GGTNACC 1 cut(s) 1816
PspFI CCCAGC 2 cut(s) 1586, 1605
PspGI CCWGG 4 cut(s) 56, 223, 574, 769
PspN4I GGNNCC 4 cut(s) 562, 613, 832, 833
PspPI GGNCC 5 cut(s) 560, 612, 831, 1181, 1608
PspPPI RGGWCCY 1 cut(s) 831
PstNI CAGNNNCTG 1 cut(s) 688
PsuI RGATCY 2 cut(s) 20, 1027
PvuI CGATCG 1 cut(s) 880
RsaI GTAC 5 cut(s) 347, 607, 647, 764, 805
RsaNI GTAC 5 cut(s) 346, 606, 646, 763, 804
RseI CAYNNNNRTG 2 cut(s) 1363, 1616
SaqAI TTAA 7 cut(s) 362, 665, 813, 1571, 1637, 1782, 1893
Sau3AI GATC 9 cut(s) 20, 520, 877, 1009, 1027, 1167, 1559, 1710, 1811
Sau96I GGNCC 5 cut(s) 560, 612, 831, 1181, 1608
ScaI AGTACT 1 cut(s) 805
SchI GAGTC 3 cut(s) 410, 1057, 1385
ScrFI CCNGG 4 cut(s) 58, 225, 576, 771
SduI GDGCHC 1 cut(s) 777
SfaNI GCATC 9 cut(s) 22, 188, 334, 521, 643, 665, 723, 1026, 1653
SfuI TTCGAA 3 cut(s) 403, 937, 1694
SinI GGWCC 1 cut(s) 831
SmiMI CAYNNNNRTG 2 cut(s) 1363, 1616
SmlI CTYRAG 3 cut(s) 664, 1679, 1803
SmoI CTYRAG 3 cut(s) 664, 1679, 1803
SphI GCATGC 1 cut(s) 1003
SseBI AGGCCT 1 cut(s) 56
SsiI CCGC 6 cut(s) 558, 1333, 1459, 1526, 1578, 1666
SspMI CTAG 4 cut(s) 81, 329, 1097, 1740
StuI AGGCCT 1 cut(s) 56
StyD4I CCNGG 4 cut(s) 56, 223, 574, 769
StyI CCWWGG 5 cut(s) 825, 1113, 1314, 1364, 1425
TaaI ACNGT 2 cut(s) 1569, 1618
TaiI ACGT 4 cut(s) 202, 1022, 1748, 1758
TaqI TCGA 8 cut(s) 403, 876, 937, 955, 1152, 1694, 1713, 1848
TaqII GACCGA 3 cut(s) 685, 1138, 1804
TatI WGTACW 1 cut(s) 803
TauI GCSGC 2 cut(s) 561, 1462
TfiI GAWTC 3 cut(s) 494, 704, 949
Tru1I TTAA 7 cut(s) 362, 665, 813, 1571, 1637, 1782, 1893
Tru9I TTAA 7 cut(s) 362, 665, 813, 1571, 1637, 1782, 1893
TscAI CASTG 4 cut(s) 76, 523, 825, 1194
TseFI GTSAC 3 cut(s) 83, 1356, 1816
TseI GCWGC 8 cut(s) 317, 734, 1003, 1286, 1289, 1445, 1501, 1797
Tsp45I GTSAC 3 cut(s) 83, 1356, 1816
TspDTI ATGAA 2 cut(s) 107, 1173
TspRI CASTG 4 cut(s) 76, 523, 825, 1194
Vha464I CTTAAG 1 cut(s) 664
VpaK11BI GGWCC 1 cut(s) 831
XagI CCTNNNNNAGG 1 cut(s) 1235
XapI RAATTY 5 cut(s) 275, 917, 979, 1092, 1766
XbaI TCTAGA 2 cut(s) 328, 1096
XceI RCATGY 1 cut(s) 1003
XmiI GTMKAC 2 cut(s) 931, 1592
XmnI GAANNNNTTC 1 cut(s) 381
XspI CTAG 4 cut(s) 81, 329, 1097, 1740
ZrmI AGTACT 1 cut(s) 805
Zsp2I ATGCAT 2 cut(s) 37, 658
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.