MD10G1254600.v1.1

Belongs to the NPH3 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0014576
Physical Location & Seq
Reverse (-)
34694812 .. 34698866
4055 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1254600.v1.1.491

Sequence Viewer

Length: 1902 bp
ATGGCTTGCGTGAAATTGGGATCTAAAACTGATGCATTCCATAGACAAGGACAGGCCTGGTTCTGCACCACTGGGCTTCCTAGTGACATTGTTGTTGAGGTTGGAGAGATGTCATTCCATCTTCATAAGTTTCCTTTGCTCTCAAGAAGTGGGGTTATGGAAAGATTGATTTCTGAAGCATCTGAAGGAGAAGAAGGATGTGTCATAACCGTCCCTGATATTCCTGGTGGGGCAAAAACATTTGAATTGGTAGCCAAGTTCTGCTACGGGGTGAAATTTGAGCTTTCTGCCTCAAATGTTGTCTACCTTCGATGTGCTGCTGAGCATCTTGAAATGACTGAAGACTACGGGGAGGACAACTTAATCACACAAACTGAAGCCTTTCTCAGTCAAGTAGTCCTTCGAAATTGGAAAGACTCTCTAAAGGCGCTTCAGACTTGTGATGATATTCTCCCTTATGCTGAAGAACTCAACATTACCAAAAGGTGCATCGAGTCACTAGCCACAAAAGCATCTACTGATCCTAATTTGTTTGGGTGGCCTGTCAGGGAGCATGGTGGCCCCATGCAAAGTCCTGGTGGGAGTGTCCTGTGGAATGGGATAAGTACAGGGGCTAGACCAAAGAAGTCAAGTTCAGATTGGTGGTATGAGGATGCATCAACCTTAAGCTTGCCTCTCTACCAGAGGCTGATTTCGGTCATGGAATCTCGTGGCATCAAACAGGAGATCATTGCAGCCTCAATCACTTATTATGCAAAAAAGTACCTTCCTGGGCTGAATCGGCGTCAAGGCAATGATTCTAGTACTCATCTTAAACCAGTGTCCTTGGGGTCCCCACCATCAGAAGAAGACCAGAAGCTCTTACTTGAAGAGGTTGATCGGTTACTTCCATTTCAGAAGCGTGTAGTCCCAACCAAATTTTTGTTTGGTCTACTTCGAACAGCCATGATTCTTCGAGCCAACCCTTCCTGCATATCAAATTTTGAGAAAAGAATTGGCATGCAGCTTGATCAAGCTACTTTGGAAGATCTTTTGATGCCCAACTTCTCTTATTCTATGGAGACTCTGTACAATGTTGACTGTGTGCAACGAATTCTTGACCACTTCATTGCCATGGAACAAACAGGAGGAGTGTCTCCATGTTCGGTCGATGATGAACAATTGATCGGGTCTTCAGGCCCTTCACTGACCCCAATCACAATGGTAGCCAAGCTTATCGATGGATACCTTGCAGAGGTTGCCCCTGATGTCAACTTGAAGCTCCCCAAGTTTCAGGCTCTTGCTGCTGCTGTTCCAGAATATGCCAGACCCTTGGATGATGGTCTTTACCGTGCCATGGATATTTATTTGAAGTCGCACCCATGGTTGGTAGAGTCCGAAAGGGAACAGCTTTGCCGGTTGATGGACTGCCAGAAGCTTTCCTTGGAAGCTTGCACTCATGCTGCACAGAATGAGAGGCTACCCTTGAGAATAGTTGTTCAAGTTCTCTTCTTCGAGCAGCTTCAGCTTAGGTCGTCAATTGCCGACTGCTTCCTGGTATCAGAAAATCTTGATGGATCAAGACAGTTAAGAAGCGGGTTTGCTATGTCTAACGAGGGAGGCTGGGCCACTACCGTGAGGGAGAATCAGGTTTTAAAGGTTGGGATGGACAACATGAGGATGCGGGTATCCGAGCTTGAGAAGGAGTGTTCGAACATGAGACAAGAGATCGAGAAGTTGGGTCGCGTAAAAGGGTCTAGCACGTGGGGAAACGTATCAAAGAAATTCGGGTTCAAGATTAAGTCCCAGATGTGCAGTGCTCAAGAGGGATCCGTCACCAATCAACACAACGCGATTGAGAAAGTCGAGAAGCTAAAGGAGAGGCATGGAAAGCATAAGAAAGACTCTGTTAAAGATGAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
BTB NPH3

Protein Analysis

634

Amino Acids

70.82

Weight (kDa)

5.88

Isoelectric Point (pI)

45.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BTB PF00651 26 - 114 5.4e-08 BTB/POZ domain
NPH3 PF03000 213 - 478 6.3e-97 NPH3 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014576)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G30440
fragaria_vesca FvH4_3g09890 FvH4_3g09890
malus_domestica MD05G1275200.v1.1 MD10G1254600.v1.1
prunus_persica Prupe.4G088800_v2.0.a1
pyrus_communis pycom05g25650 pycom10g21020
rosa_chinensis RchiOBHm_Chr5g0015601
rosa_laevigata RLG00000023153 RLG00000032212
rosa_multiflora Rmu_sc0003230.1_g000001
rosa_roxburghii Rroxscaffold_1G00060870
rosa_rugosa Rorug05G0028800 Rorug05G0028800
rosa_samantha Rh5AG122100
rosa_wichuraiana Rw5G010530

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 303, 931
AccII CGCG 2 cut(s) 1725, 1832
AciI CCGC 2 cut(s) 1575, 1663
AclWI GGATC 5 cut(s) 28, 515, 1564, 1803, 1816
AcsI RAATTY 5 cut(s) 275, 917, 979, 1092, 1763
AcuI CTGAAG 8 cut(s) 195, 204, 360, 396, 416, 483, 1158, 1487
AcvI CACGTG 1 cut(s) 1743
AcyI GRCGYC 1 cut(s) 784
AfaI GTAC 4 cut(s) 607, 764, 805, 1070
AfiI CCNNNNNNNGG 4 cut(s) 1234, 1336, 1366, 1402
AflII CTTAAG 1 cut(s) 664
AgsI TTSAA 7 cut(s) 245, 332, 869, 1258, 1351, 1481, 1774
AjnI CCWGG 5 cut(s) 56, 223, 574, 769, 1533
AjuI GAANNNNNNNTTGG 2 cut(s) 1406, 1438
AleI CACNNNNGTG 1 cut(s) 1613
Alw21I GWGCWC 1 cut(s) 1801
Alw26I GTCTC 3 cut(s) 1055, 1140, 1693
AlwI GGATC 5 cut(s) 28, 515, 1564, 1803, 1816
AlwNI CAGNNNCTG 1 cut(s) 688
AoxI GGCC 5 cut(s) 54, 539, 559, 1177, 1605
ApeKI GCWGC 7 cut(s) 317, 734, 1003, 1283, 1286, 1442, 1498
ApoI RAATTY 5 cut(s) 275, 917, 979, 1092, 1763
Asp700I GAANNNNTTC 1 cut(s) 381
AspLEI GCGC 1 cut(s) 430
AspS9I GGNCC 4 cut(s) 560, 831, 1178, 1605
AsuHPI GGTGA 2 cut(s) 283, 1807
AsuII TTCGAA 3 cut(s) 403, 937, 1691
AvaII GGWCC 1 cut(s) 831
BamHI GGATCC 1 cut(s) 1808
BauI CACGAG 1 cut(s) 708
BbrPI CACGTG 1 cut(s) 1743
BbsI GAAGAC 3 cut(s) 348, 855, 1164
Bbv12I GWGCWC 1 cut(s) 1801
BbvI GCAGC 7 cut(s) 304, 746, 1015, 1270, 1273, 1429, 1510
BccI CCATC 7 cut(s) 126, 847, 1214, 1313, 1396, 1547, 1639
BciT130I CCWGG 5 cut(s) 58, 225, 576, 771, 1535
BciVI GTATCC 2 cut(s) 1217, 1678
BclI TGATCA 1 cut(s) 1009
BcoDI GTCTC 3 cut(s) 1055, 1140, 1693
BfaI CTAG 5 cut(s) 81, 500, 615, 801, 1737
BfoI RGCGCY 1 cut(s) 431
BfrI CTTAAG 1 cut(s) 664
BfuI GTATCC 2 cut(s) 1217, 1678
BglII AGATCT 1 cut(s) 1027
BisI GCNGC 7 cut(s) 318, 735, 1004, 1284, 1287, 1443, 1499
BlpI GCTNAGC 1 cut(s) 321
BlsI GCNGC 7 cut(s) 319, 736, 1005, 1285, 1288, 1444, 1500
BmcAI AGTACT 1 cut(s) 805
Bme1390I CCNGG 5 cut(s) 58, 225, 576, 771, 1535
Bme18I GGWCC 1 cut(s) 831
BmgT120I GGNCC 4 cut(s) 560, 831, 1178, 1605
BmiI GGNNCC 4 cut(s) 562, 832, 833, 1810
BmrFI CCNGG 5 cut(s) 58, 225, 576, 771, 1535
BmrI ACTGGG 1 cut(s) 81
BmuI ACTGGG 1 cut(s) 81
BpiI GAAGAC 3 cut(s) 348, 855, 1164
Bpu10I CCTNAGC 1 cut(s) 1508
Bpu1102I GCTNAGC 1 cut(s) 321
Bpu14I TTCGAA 3 cut(s) 403, 937, 1691
BpuEI CTTGAG 4 cut(s) 127, 1486, 1697, 1785
Bsa29I ATCGAT 1 cut(s) 1218
BsaAI YACGTR 1 cut(s) 1743
BsaHI GRCGYC 1 cut(s) 784
BsaJI CCNNGG 7 cut(s) 770, 825, 1113, 1311, 1335, 1361, 1422
BsaXI ACNNNNNCTCC 2 cut(s) 1052, 1082
Bsc4I CCNNNNNNNGG 4 cut(s) 1234, 1336, 1366, 1402
Bse118I RCCGGY 1 cut(s) 1395
Bse1I ACTGG 2 cut(s) 76, 818
Bse3DI GCAATG 3 cut(s) 729, 799, 1107
BseBI CCWGG 5 cut(s) 58, 225, 576, 771, 1535
BseCI ATCGAT 1 cut(s) 1218
BseDI CCNNGG 7 cut(s) 770, 825, 1113, 1311, 1335, 1361, 1422
BseGI GGATG 5 cut(s) 203, 658, 1321, 1650, 1665
BseLI CCNNNNNNNGG 4 cut(s) 1234, 1336, 1366, 1402
BseMI GCAATG 3 cut(s) 729, 799, 1107
BseMII CTCAG 2 cut(s) 312, 400
BseNI ACTGG 2 cut(s) 76, 818
BseRI GAGGAG 1 cut(s) 1143
BseXI GCAGC 7 cut(s) 304, 746, 1015, 1270, 1273, 1429, 1510
BseYI CCCAGC 1 cut(s) 1602
BsgI GTGCAG 3 cut(s) 49, 1428, 1813
Bsh1236I CGCG 2 cut(s) 1725, 1832
Bsh1285I CGRYCG 1 cut(s) 1149
BshFI GGCC 5 cut(s) 56, 541, 561, 1179, 1607
BshVI ATCGAT 1 cut(s) 1218
BsiEI CGRYCG 1 cut(s) 1149
BsiHKAI GWGCWC 1 cut(s) 1801
BsiSI CCGG 1 cut(s) 1396
BslFI GGGAC 4 cut(s) 197, 817, 893, 1768
BslI CCNNNNNNNGG 4 cut(s) 1234, 1336, 1366, 1402
BsmAI GTCTC 3 cut(s) 1055, 1140, 1693
BsmFI GGGAC 4 cut(s) 197, 817, 893, 1768
BsmI GAATGC 1 cut(s) 35
BsnI GGCC 5 cut(s) 56, 541, 561, 1179, 1607
Bsp119I TTCGAA 3 cut(s) 403, 937, 1691
Bsp1286I GDGCHC 1 cut(s) 1801
Bsp1407I TGTACA 1 cut(s) 1068
Bsp1720I GCTNAGC 1 cut(s) 321
Bsp19I CCATGG 3 cut(s) 1113, 1335, 1361
BspACI CCGC 2 cut(s) 1575, 1663
BspANI GGCC 5 cut(s) 56, 541, 561, 1179, 1607
BspCNI CTCAG 2 cut(s) 313, 399
BspDI ATCGAT 1 cut(s) 1218
BspFNI CGCG 2 cut(s) 1725, 1832
BspLI GGNNCC 4 cut(s) 562, 832, 833, 1810
BspPI GGATC 5 cut(s) 28, 515, 1564, 1803, 1816
BspT104I TTCGAA 3 cut(s) 403, 937, 1691
BspTI CTTAAG 1 cut(s) 664
BsrDI GCAATG 3 cut(s) 729, 799, 1107
BsrFI RCCGGY 1 cut(s) 1395
BsrGI TGTACA 1 cut(s) 1068
BsrI ACTGG 2 cut(s) 76, 818
BssAI RCCGGY 1 cut(s) 1395
BssECI CCNNGG 7 cut(s) 770, 825, 1113, 1311, 1335, 1361, 1422
BssNI GRCGYC 1 cut(s) 784
BssSI CACGAG 1 cut(s) 708
BssT1I CCWWGG 6 cut(s) 825, 1113, 1311, 1335, 1361, 1422
Bst2BI CACGAG 1 cut(s) 708
Bst2UI CCWGG 5 cut(s) 58, 225, 576, 771, 1535
Bst4CI ACNGT 5 cut(s) 211, 1082, 1331, 1566, 1615
Bst6I CTCTTC 2 cut(s) 864, 1493
BstACI GRCGYC 1 cut(s) 784
BstAFI CTTAAG 1 cut(s) 664
BstAPI GCANNNNNTGC 1 cut(s) 1238
BstAUI TGTACA 1 cut(s) 1068
BstBAI YACGTR 1 cut(s) 1743
BstBI TTCGAA 3 cut(s) 403, 937, 1691
BstC8I GCNNGC 4 cut(s) 7, 671, 1001, 1432
BstDEI CTNAG 3 cut(s) 321, 386, 1508
BstDSI CCRYGG 3 cut(s) 1113, 1335, 1361
BstENI CCTNNNNNAGG 1 cut(s) 1232
BstF5I GGATG 5 cut(s) 203, 658, 1321, 1650, 1665
BstFNI CGCG 2 cut(s) 1725, 1832
BstH2I RGCGCY 1 cut(s) 431
BstHHI GCGC 1 cut(s) 430
BstMAI GTCTC 3 cut(s) 1055, 1140, 1693
BstMCI CGRYCG 1 cut(s) 1149
BstMWI GCNNNNNNNGC 6 cut(s) 509, 781, 1238, 1283, 1504, 1870
BstNI CCWGG 5 cut(s) 58, 225, 576, 771, 1535
BstNSI RCATGY 1 cut(s) 1003
BstSCI CCNGG 5 cut(s) 56, 223, 574, 769, 1533
BstUI CGCG 2 cut(s) 1725, 1832
BstV1I GCAGC 7 cut(s) 304, 746, 1015, 1270, 1273, 1429, 1510
BstV2I GAAGAC 3 cut(s) 348, 855, 1164
BstX2I RGATCY 3 cut(s) 20, 1027, 1808
BstXI CCANNNNNNTGG 1 cut(s) 1312
BstYI RGATCY 3 cut(s) 20, 1027, 1808
Bsu15I ATCGAT 1 cut(s) 1218
BsuI GTATCC 2 cut(s) 1217, 1678
BsuRI GGCC 5 cut(s) 56, 541, 561, 1179, 1607
BsuTUI ATCGAT 1 cut(s) 1218
BtgI CCRYGG 3 cut(s) 1113, 1335, 1361
BtsCI GGATG 5 cut(s) 203, 658, 1321, 1650, 1665
BtsI GCAGTG 1 cut(s) 1801
BtsIMutI CAGTG 4 cut(s) 69, 825, 1184, 1801
Cac8I GCNNGC 4 cut(s) 7, 671, 1001, 1432
CaiI CAGNNNCTG 1 cut(s) 688
CfoI GCGC 1 cut(s) 430
Cfr10I RCCGGY 1 cut(s) 1395
Cfr13I GGNCC 4 cut(s) 560, 831, 1178, 1605
ClaI ATCGAT 1 cut(s) 1218
CseI GACGC 1 cut(s) 773
Csp6I GTAC 4 cut(s) 606, 763, 804, 1069
CviQI GTAC 4 cut(s) 606, 763, 804, 1069
DdeI CTNAG 3 cut(s) 321, 386, 1508
DraI TTTAAA 1 cut(s) 1635
Eam1104I CTCTTC 2 cut(s) 864, 1493
EarI CTCTTC 2 cut(s) 864, 1493
Eco130I CCWWGG 6 cut(s) 825, 1113, 1311, 1335, 1361, 1422
Eco147I AGGCCT 1 cut(s) 56
Eco47I GGWCC 1 cut(s) 831
Eco57I CTGAAG 8 cut(s) 195, 204, 360, 396, 416, 483, 1158, 1487
Eco72I CACGTG 1 cut(s) 1743
EcoNI CCTNNNNNAGG 1 cut(s) 1232
EcoO109I RGGNCCY 2 cut(s) 831, 1178
EcoRI GAATTC 1 cut(s) 1092
EcoRII CCWGG 5 cut(s) 56, 223, 574, 769, 1533
EcoT14I CCWWGG 6 cut(s) 825, 1113, 1311, 1335, 1361, 1422
EcoT22I ATGCAT 2 cut(s) 37, 658
ErhI CCWWGG 6 cut(s) 825, 1113, 1311, 1335, 1361, 1422
FalI AAGNNNNNCTT 4 cut(s) 384, 416, 1406, 1438
FaqI GGGAC 4 cut(s) 197, 817, 893, 1768
FauI CCCGC 2 cut(s) 1568, 1656
FbaI TGATCA 1 cut(s) 1009
FblI GTMKAC 2 cut(s) 303, 931
Fnu4HI GCNGC 7 cut(s) 318, 735, 1004, 1284, 1287, 1443, 1499
FokI GGATG 5 cut(s) 210, 665, 1328, 1657, 1672
Fsp4HI GCNGC 7 cut(s) 318, 735, 1004, 1284, 1287, 1443, 1499
FspBI CTAG 5 cut(s) 81, 500, 615, 801, 1737
GlaI GCGC 1 cut(s) 429
GluI GCNGC 7 cut(s) 318, 735, 1004, 1284, 1287, 1443, 1499
GsaI CCCAGC 1 cut(s) 1606
HaeII RGCGCY 1 cut(s) 431
HaeIII GGCC 5 cut(s) 56, 541, 561, 1179, 1607
HapII CCGG 1 cut(s) 1396
HgaI GACGC 1 cut(s) 773
HhaI GCGC 1 cut(s) 430
Hin1I GRCGYC 1 cut(s) 784
Hin6I GCGC 1 cut(s) 428
HinP1I GCGC 1 cut(s) 428
HincII GTYRAC 2 cut(s) 1078, 1252
HindII GTYRAC 2 cut(s) 1078, 1252
HindIII AAGCTT 4 cut(s) 667, 1211, 1415, 1428
HpaII CCGG 1 cut(s) 1396
HphI GGTGA 2 cut(s) 283, 1807
Hpy166II GTNNAC 4 cut(s) 304, 932, 1078, 1252
Hpy188I TCNGA 9 cut(s) 175, 184, 435, 637, 844, 897, 1378, 1543, 1672
Hpy8I GTNNAC 4 cut(s) 304, 932, 1078, 1252
HpyAV CCTTC 8 cut(s) 179, 188, 317, 410, 776, 975, 1191, 1675
HpyCH4III ACNGT 5 cut(s) 211, 1082, 1331, 1566, 1615
HpyCH4IV ACGT 2 cut(s) 1742, 1752
HpyF10VI GCNNNNNNNGC 6 cut(s) 509, 781, 1238, 1283, 1504, 1870
HpyF3I CTNAG 3 cut(s) 321, 386, 1508
HpySE526I ACGT 2 cut(s) 1742, 1752
Hsp92I GRCGYC 1 cut(s) 784
HspAI GCGC 1 cut(s) 428
KflI GGGWCCC 1 cut(s) 831
Ksp22I TGATCA 1 cut(s) 1009
LmnI GCTCC 2 cut(s) 550, 1266
Lsp1109I GCAGC 7 cut(s) 304, 746, 1015, 1270, 1273, 1429, 1510
MaeI CTAG 5 cut(s) 81, 500, 615, 801, 1737
MaeII ACGT 2 cut(s) 1742, 1752
MaeIII GTNAC 4 cut(s) 83, 495, 882, 1813
MfeI CAATTG 2 cut(s) 1160, 1518
MflI RGATCY 3 cut(s) 20, 1027, 1808
MhlI GDGCHC 1 cut(s) 1801
MlyI GAGTC 5 cut(s) 410, 503, 1057, 1382, 1877
MmeI TCCRAC 1 cut(s) 82
Mph1103I ATGCAT 2 cut(s) 37, 658
MroXI GAANNNNTTC 1 cut(s) 381
MseI TTAA 7 cut(s) 362, 665, 813, 1568, 1634, 1779, 1890
MslI CAYNNNNRTG 3 cut(s) 1112, 1613, 1658
MspCI CTTAAG 1 cut(s) 664
MspI CCGG 1 cut(s) 1396
MspR9I CCNGG 5 cut(s) 58, 225, 576, 771, 1535
MunI CAATTG 2 cut(s) 1160, 1518
Mva1269I GAATGC 1 cut(s) 35
MvaI CCWGG 5 cut(s) 58, 225, 576, 771, 1535
MvnI CGCG 2 cut(s) 1725, 1832
MwoI GCNNNNNNNGC 6 cut(s) 509, 781, 1238, 1283, 1504, 1870
NcoI CCATGG 3 cut(s) 1113, 1335, 1361
NlaIV GGNNCC 4 cut(s) 562, 832, 833, 1810
NmuCI GTSAC 3 cut(s) 83, 495, 1813
NsiI ATGCAT 2 cut(s) 37, 658
NspI RCATGY 1 cut(s) 1003
NspV TTCGAA 3 cut(s) 403, 937, 1691
OliI CACNNNNGTG 1 cut(s) 1613
PaeI GCATGC 1 cut(s) 1003
PceI AGGCCT 1 cut(s) 56
PctI GAATGC 1 cut(s) 35
PdmI GAANNNNTTC 1 cut(s) 381
PfeI GAWTC 5 cut(s) 704, 778, 797, 949, 1624
PkrI GCNGC 7 cut(s) 319, 736, 1005, 1285, 1288, 1444, 1500
PleI GAGTC 5 cut(s) 410, 502, 1057, 1381, 1877
PmaCI CACGTG 1 cut(s) 1743
PmlI CACGTG 1 cut(s) 1743
PpsI GAGTC 5 cut(s) 410, 502, 1057, 1381, 1877
Ppu21I YACGTR 1 cut(s) 1743
PpuMI RGGWCCY 1 cut(s) 831
Psp5II RGGWCCY 1 cut(s) 831
Psp6I CCWGG 5 cut(s) 56, 223, 574, 769, 1533
PspCI CACGTG 1 cut(s) 1743
PspFI CCCAGC 1 cut(s) 1602
PspGI CCWGG 5 cut(s) 56, 223, 574, 769, 1533
PspN4I GGNNCC 4 cut(s) 562, 832, 833, 1810
PspPI GGNCC 4 cut(s) 560, 831, 1178, 1605
PspPPI RGGWCCY 1 cut(s) 831
PstNI CAGNNNCTG 1 cut(s) 688
PsuI RGATCY 3 cut(s) 20, 1027, 1808
RsaI GTAC 4 cut(s) 607, 764, 805, 1070
RsaNI GTAC 4 cut(s) 606, 763, 804, 1069
RseI CAYNNNNRTG 3 cut(s) 1112, 1613, 1658
SaqAI TTAA 7 cut(s) 362, 665, 813, 1568, 1634, 1779, 1890
SatI GCNGC 7 cut(s) 318, 735, 1004, 1284, 1287, 1443, 1499
Sau96I GGNCC 4 cut(s) 560, 831, 1178, 1605
ScaI AGTACT 1 cut(s) 805
SchI GAGTC 5 cut(s) 410, 503, 1057, 1382, 1877
ScrFI CCNGG 5 cut(s) 58, 225, 576, 771, 1535
SduI GDGCHC 1 cut(s) 1801
SfuI TTCGAA 3 cut(s) 403, 937, 1691
SinI GGWCC 1 cut(s) 831
SmiMI CAYNNNNRTG 3 cut(s) 1112, 1613, 1658
SmlI CTYRAG 5 cut(s) 142, 664, 1465, 1676, 1800
SmoI CTYRAG 5 cut(s) 142, 664, 1465, 1676, 1800
SphI GCATGC 1 cut(s) 1003
SseBI AGGCCT 1 cut(s) 56
SsiI CCGC 2 cut(s) 1575, 1663
SspMI CTAG 5 cut(s) 81, 500, 615, 801, 1737
StuI AGGCCT 1 cut(s) 56
StyD4I CCNGG 5 cut(s) 56, 223, 574, 769, 1533
StyI CCWWGG 6 cut(s) 825, 1113, 1311, 1335, 1361, 1422
TaaI ACNGT 5 cut(s) 211, 1082, 1331, 1566, 1615
TaiI ACGT 2 cut(s) 1745, 1755
TaqII GACCGA 2 cut(s) 685, 1135
TatI WGTACW 3 cut(s) 605, 803, 1068
TfiI GAWTC 5 cut(s) 704, 778, 797, 949, 1624
Tru1I TTAA 7 cut(s) 362, 665, 813, 1568, 1634, 1779, 1890
Tru9I TTAA 7 cut(s) 362, 665, 813, 1568, 1634, 1779, 1890
TscAI CASTG 4 cut(s) 76, 825, 1191, 1801
TseFI GTSAC 3 cut(s) 83, 495, 1813
TseI GCWGC 7 cut(s) 317, 734, 1003, 1283, 1286, 1442, 1498
Tsp45I GTSAC 3 cut(s) 83, 495, 1813
TspDTI ATGAA 3 cut(s) 113, 1096, 1170
TspGWI ACGGA 1 cut(s) 1801
TspRI CASTG 4 cut(s) 76, 825, 1191, 1801
Vha464I CTTAAG 1 cut(s) 664
VpaK11BI GGWCC 1 cut(s) 831
XagI CCTNNNNNAGG 1 cut(s) 1232
XapI RAATTY 5 cut(s) 275, 917, 979, 1092, 1763
XceI RCATGY 1 cut(s) 1003
XmiI GTMKAC 2 cut(s) 303, 931
XmnI GAANNNNTTC 1 cut(s) 381
XspI CTAG 5 cut(s) 81, 500, 615, 801, 1737
ZrmI AGTACT 1 cut(s) 805
Zsp2I ATGCAT 2 cut(s) 37, 658
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.