MD05G1279400.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0015570
Physical Location & Seq
Reverse (-)
41365088 .. 41366796
1709 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1279400.v1.1.491

Sequence Viewer

Length: 759 bp
ATGGCATGCTTTGTGCCTTTCAACAGTAAAAATTTAGACATAAGCATCTTCGTGTTCAGGCCAACAATTGTGTTGGTCGATGACCTTCTTAATGCTCTCAAGCAATTCTCTTTGTGCACTGAGTCTCTTGGTTGTGTTCAAAGTTCAATCTTGCAGAGTATCCATGGAAATATGTTCATATGGTTTGGAGCGTGGCTGAAGAGAACATGTGAAAACAAAGATTCATTGACTGCACGCCTTCTCTCAATGTTAGCGAATATATCAAACATGGCTATCTTAGTCGATCACTCCTTCTTCGACGCGTATGCTGGAGAATCGAGAGACGGTTCTTCTGCAGCGAAATTTCGCAGAGGTGACACGGTGTCACTGAGCACTGCCCTCATCTACGGTGGTGACATAAACAACGTCTCTTACGCATGCTTAGCGTTGTTCAAGTCCGGTTTTCAAAGGATGCAAGGCGCGACAGCCGGTGTTTGTTTGAAATGCCAAACCGGGCAAAGAACTGCTTCTCTCTTCGTGTGGAAGTCTCTGCAATATTGCTACTCATGGATTCTAAATTCCGACCAAAGAAAAGCATTGTCGCCCTATCTCGAAAGCGTCTCGATCGAGATTAAGTACGACATTTTTCGAGTGGTCTATGTCAGCGGCGACAGTGATATCGACTTGCAGGTCTTATATCCTCGTCATCAGATGTTAGAACAAGCTGGAGGCGAAAGCAAAGAAGAAGGGCAGCTCATGCAAAGTACTTCCATAGTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

253

Amino Acids

28.05

Weight (kDa)

6.3

Isoelectric Point (pI)

48.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7392 PF24118 97 - 209 1.6e-50 Domain of unknown function (DUF7392)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015570)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g05030
malus_domestica MD05G1279400.v1.1 MD10G1258100.v1.1
prunus_persica Prupe.4G085700_v2.0.a1
pyrus_communis pycom05g25970 pycom10g21440
rosa_chinensis RchiOBHm_Chr5g0014941
rosa_laevigata RLG00000032152
rosa_multiflora Rmu_ssc0000388.1_g000067
rosa_roxburghii Rroxscaffold_1G00061640
rosa_rugosa Rorug05G0021700
rosa_samantha Rh5AG117300 Rh5BG114300 Rh5CG125800 Rh5DG113700
rosa_wichuraiana Rw5G010100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 668
Acc36I ACCTGC 1 cut(s) 658
AccII CGCG 2 cut(s) 302, 461
AciI CCGC 1 cut(s) 645
AcsI RAATTY 3 cut(s) 31, 341, 556
AcuI CTGAAG 1 cut(s) 218
AfaI GTAC 2 cut(s) 617, 745
AflIII ACRYGT 2 cut(s) 206, 300
AgsI TTSAA 6 cut(s) 22, 140, 147, 433, 446, 481
AhdI GACNNNNNGTC 1 cut(s) 361
AluBI AGCT 2 cut(s) 704, 733
AluI AGCT 2 cut(s) 704, 733
Alw21I GWGCWC 2 cut(s) 119, 374
Alw26I GTCTC 5 cut(s) 129, 315, 412, 531, 604
Alw44I GTGCAC 1 cut(s) 115
AoxI GGCC 1 cut(s) 59
ApaLI GTGCAC 1 cut(s) 115
ApeKI GCWGC 2 cut(s) 335, 730
ApoI RAATTY 3 cut(s) 31, 341, 556
Asp700I GAANNNNTTC 1 cut(s) 505
AspLEI GCGC 1 cut(s) 461
AsuC2I CCSGG 1 cut(s) 493
AsuHPI GGTGA 2 cut(s) 365, 404
BaeGI GKGCMC 1 cut(s) 119
Bbv12I GWGCWC 2 cut(s) 119, 374
BbvI GCAGC 2 cut(s) 347, 742
BcgI CGANNNNNNTGC 2 cut(s) 287, 321
BciVI GTATCC 1 cut(s) 170
BcnI CCSGG 1 cut(s) 493
BcoDI GTCTC 5 cut(s) 129, 315, 412, 531, 604
BfmI CTRYAG 1 cut(s) 333
BfuAI ACCTGC 1 cut(s) 658
BfuI GTATCC 1 cut(s) 170
BisI GCNGC 3 cut(s) 336, 646, 731
BlpI GCTNAGC 1 cut(s) 421
BlsI GCNGC 3 cut(s) 337, 647, 732
BmcAI AGTACT 1 cut(s) 745
Bme1390I CCNGG 1 cut(s) 493
BmeRI GACNNNNNGTC 1 cut(s) 361
BmrFI CCNGG 1 cut(s) 493
BmsI GCATC 2 cut(s) 54, 441
BpmI CTGGAG 2 cut(s) 330, 726
Bpu1102I GCTNAGC 1 cut(s) 421
BpuEI CTTGAG 1 cut(s) 83
BpuMI CCSGG 1 cut(s) 493
BsaJI CCNNGG 1 cut(s) 163
BsaWI WCCGGW 1 cut(s) 437
Bse118I RCCGGY 1 cut(s) 467
BseDI CCNNGG 1 cut(s) 163
BseGI GGATG 1 cut(s) 456
BseMII CTCAG 2 cut(s) 111, 359
BseSI GKGCMC 1 cut(s) 119
BseXI GCAGC 2 cut(s) 347, 742
BsgI GTGCAG 1 cut(s) 216
Bsh1236I CGCG 2 cut(s) 302, 461
Bsh1285I CGRYCG 1 cut(s) 606
BshFI GGCC 1 cut(s) 61
BsiEI CGRYCG 1 cut(s) 606
BsiHKAI GWGCWC 2 cut(s) 119, 374
BsiSI CCGG 3 cut(s) 438, 468, 492
BsmAI GTCTC 5 cut(s) 129, 315, 412, 531, 604
BsmBI CGTCTC 3 cut(s) 315, 412, 604
BsnI GGCC 1 cut(s) 61
Bsp1286I GDGCHC 2 cut(s) 119, 374
Bsp143I GATC 2 cut(s) 283, 603
Bsp1720I GCTNAGC 1 cut(s) 421
Bsp19I CCATGG 1 cut(s) 163
BspACI CCGC 1 cut(s) 645
BspANI GGCC 1 cut(s) 61
BspCNI CTCAG 2 cut(s) 112, 360
BspFNI CGCG 2 cut(s) 302, 461
BspMAI CTGCAG 1 cut(s) 337
BspMI ACCTGC 1 cut(s) 658
BsrFI RCCGGY 1 cut(s) 467
BssAI RCCGGY 1 cut(s) 467
BssECI CCNNGG 1 cut(s) 163
BssMI GATC 2 cut(s) 283, 603
BssT1I CCWWGG 1 cut(s) 163
Bst4CI ACNGT 5 cut(s) 26, 326, 361, 389, 653
Bst6I CTCTTC 2 cut(s) 194, 518
BstAPI GCANNNNNTGC 1 cut(s) 736
BstC8I GCNNGC 3 cut(s) 7, 235, 418
BstDEI CTNAG 4 cut(s) 120, 277, 368, 421
BstDSI CCRYGG 1 cut(s) 163
BstF5I GGATG 1 cut(s) 456
BstFNI CGCG 2 cut(s) 302, 461
BstHHI GCGC 1 cut(s) 461
BstKTI GATC 2 cut(s) 286, 606
BstMAI GTCTC 5 cut(s) 129, 315, 412, 531, 604
BstMBI GATC 2 cut(s) 283, 603
BstMCI CGRYCG 1 cut(s) 606
BstMWI GCNNNNNNNGC 2 cut(s) 422, 736
BstNSI RCATGY 3 cut(s) 9, 210, 420
BstSCI CCNGG 1 cut(s) 491
BstSFI CTRYAG 1 cut(s) 333
BstSLI GKGCMC 1 cut(s) 119
BstUI CGCG 2 cut(s) 302, 461
BstV1I GCAGC 2 cut(s) 347, 742
BsuI GTATCC 1 cut(s) 170
BsuRI GGCC 1 cut(s) 61
BtgI CCRYGG 1 cut(s) 163
BtsCI GGATG 1 cut(s) 456
BtsI GCAGTG 1 cut(s) 372
BtsIMutI CAGTG 4 cut(s) 117, 365, 372, 658
BveI ACCTGC 1 cut(s) 658
Cac8I GCNNGC 3 cut(s) 7, 235, 418
CfoI GCGC 1 cut(s) 461
Cfr10I RCCGGY 1 cut(s) 467
CseI GACGC 2 cut(s) 308, 586
Csp6I GTAC 2 cut(s) 616, 744
CviAII CATG 7 cut(s) 6, 164, 207, 268, 417, 546, 736
CviJI RGCY 6 cut(s) 61, 196, 272, 467, 704, 733
CviKI_1 RGCY 6 cut(s) 61, 196, 272, 467, 704, 733
CviQI GTAC 2 cut(s) 616, 744
DdeI CTNAG 4 cut(s) 120, 277, 368, 421
DpnI GATC 2 cut(s) 285, 605
DpnII GATC 2 cut(s) 283, 603
DrdI GACNNNNNNGTC 1 cut(s) 668
DriI GACNNNNNGTC 1 cut(s) 361
DseDI GACNNNNNNGTC 1 cut(s) 668
Eam1104I CTCTTC 2 cut(s) 194, 518
Eam1105I GACNNNNNGTC 1 cut(s) 361
EarI CTCTTC 2 cut(s) 194, 518
Eco130I CCWWGG 1 cut(s) 163
Eco32I GATATC 1 cut(s) 658
Eco57I CTGAAG 1 cut(s) 218
EcoRV GATATC 1 cut(s) 658
EcoT14I CCWWGG 1 cut(s) 163
ErhI CCWWGG 1 cut(s) 163
Esp3I CGTCTC 3 cut(s) 315, 412, 604
FaeI CATG 7 cut(s) 9, 167, 210, 271, 420, 549, 739
FalI AAGNNNNNCTT 2 cut(s) 490, 522
FatI CATG 7 cut(s) 5, 163, 206, 267, 416, 545, 735
FauNDI CATATG 1 cut(s) 179
Fnu4HI GCNGC 3 cut(s) 336, 646, 731
FokI GGATG 1 cut(s) 463
Fsp4HI GCNGC 3 cut(s) 336, 646, 731
GlaI GCGC 1 cut(s) 460
GluI GCNGC 3 cut(s) 336, 646, 731
GsuI CTGGAG 2 cut(s) 330, 726
HaeIII GGCC 1 cut(s) 61
HapII CCGG 3 cut(s) 438, 468, 492
HgaI GACGC 2 cut(s) 308, 586
HhaI GCGC 1 cut(s) 461
Hin1II CATG 7 cut(s) 9, 167, 210, 271, 420, 549, 739
Hin6I GCGC 1 cut(s) 459
HinP1I GCGC 1 cut(s) 459
HinfI GANTC 4 cut(s) 122, 221, 314, 550
HpaII CCGG 3 cut(s) 438, 468, 492
HphI GGTGA 2 cut(s) 365, 404
Hpy166II GTNNAC 1 cut(s) 117
Hpy188I TCNGA 2 cut(s) 562, 690
Hpy188III TCNNGA 4 cut(s) 318, 590, 601, 607
Hpy8I GTNNAC 1 cut(s) 117
Hpy99I CGWCG 1 cut(s) 302
HpyAV CCTTC 4 cut(s) 95, 248, 301, 719
HpyCH4III ACNGT 5 cut(s) 26, 326, 361, 389, 653
HpyCH4IV ACGT 1 cut(s) 405
HpyCH4V TGCA 8 cut(s) 117, 154, 233, 335, 454, 532, 667, 739
HpyF10VI GCNNNNNNNGC 2 cut(s) 422, 736
HpyF3I CTNAG 4 cut(s) 120, 277, 368, 421
HpySE526I ACGT 1 cut(s) 405
Hsp92II CATG 7 cut(s) 9, 167, 210, 271, 420, 549, 739
HspAI GCGC 1 cut(s) 459
Kzo9I GATC 2 cut(s) 283, 603
LmnI GCTCC 1 cut(s) 188
LpnPI CCDG 7 cut(s) 43, 294, 451, 481, 505, 653, 690
Lsp1109I GCAGC 2 cut(s) 347, 742
LweI GCATC 2 cut(s) 54, 441
MaeII ACGT 1 cut(s) 405
MaeIII GTNAC 3 cut(s) 353, 363, 392
MalI GATC 2 cut(s) 285, 605
MboI GATC 2 cut(s) 283, 603
MboII GAAGA 6 cut(s) 40, 211, 286, 321, 505, 734
MfeI CAATTG 1 cut(s) 66
MhlI GDGCHC 2 cut(s) 119, 374
MluCI AATT 5 cut(s) 31, 66, 104, 341, 556
MluI ACGCGT 1 cut(s) 300
MlyI GAGTC 1 cut(s) 131
MmeI TCCRAC 1 cut(s) 585
MnlI CCTC 4 cut(s) 344, 389, 690, 701
MroXI GAANNNNTTC 1 cut(s) 505
MseI TTAA 2 cut(s) 90, 612
MslI CAYNNNNRTG 1 cut(s) 50
MspA1I CMGCKG 1 cut(s) 645
MspI CCGG 3 cut(s) 438, 468, 492
MspR9I CCNGG 1 cut(s) 493
MunI CAATTG 1 cut(s) 66
MvnI CGCG 2 cut(s) 302, 461
MwoI GCNNNNNNNGC 2 cut(s) 422, 736
NciI CCSGG 1 cut(s) 493
NcoI CCATGG 1 cut(s) 163
NdeI CATATG 1 cut(s) 179
NdeII GATC 2 cut(s) 283, 603
NlaIII CATG 7 cut(s) 9, 167, 210, 271, 420, 549, 739
NmuCI GTSAC 3 cut(s) 353, 363, 392
NspI RCATGY 3 cut(s) 9, 210, 420
PaeI GCATGC 2 cut(s) 9, 420
PciI ACATGT 1 cut(s) 206
PdmI GAANNNNTTC 1 cut(s) 505
PfeI GAWTC 3 cut(s) 221, 314, 550
PkrI GCNGC 3 cut(s) 337, 647, 732
Ple19I CGATCG 1 cut(s) 606
PleI GAGTC 1 cut(s) 130
PpsI GAGTC 1 cut(s) 130
PscI ACATGT 1 cut(s) 206
PstI CTGCAG 1 cut(s) 337
PvuI CGATCG 1 cut(s) 606
RsaI GTAC 2 cut(s) 617, 745
RsaNI GTAC 2 cut(s) 616, 744
RseI CAYNNNNRTG 1 cut(s) 50
SaqAI TTAA 2 cut(s) 90, 612
SatI GCNGC 3 cut(s) 336, 646, 731
Sau3AI GATC 2 cut(s) 283, 603
ScaI AGTACT 1 cut(s) 745
SchI GAGTC 1 cut(s) 131
ScrFI CCNGG 1 cut(s) 493
SduI GDGCHC 2 cut(s) 119, 374
SetI ASST 6 cut(s) 87, 355, 408, 672, 706, 735
SfaNI GCATC 2 cut(s) 54, 441
SfcI CTRYAG 1 cut(s) 333
SmiMI CAYNNNNRTG 1 cut(s) 50
SmlI CTYRAG 1 cut(s) 98
SmoI CTYRAG 1 cut(s) 98
SphI GCATGC 2 cut(s) 9, 420
Sse9I AATT 5 cut(s) 31, 66, 104, 341, 556
SsiI CCGC 1 cut(s) 645
SspI AATATT 1 cut(s) 536
StyD4I CCNGG 1 cut(s) 491
StyI CCWWGG 1 cut(s) 163
TaaI ACNGT 5 cut(s) 26, 326, 361, 389, 653
TaiI ACGT 1 cut(s) 408
TaqI TCGA 9 cut(s) 78, 282, 297, 317, 591, 602, 606, 628, 660
TasI AATT 5 cut(s) 31, 66, 104, 341, 556
TatI WGTACW 1 cut(s) 743
TauI GCSGC 1 cut(s) 648
TfiI GAWTC 3 cut(s) 221, 314, 550
Tru1I TTAA 2 cut(s) 90, 612
Tru9I TTAA 2 cut(s) 90, 612
TscAI CASTG 4 cut(s) 124, 372, 379, 658
TseFI GTSAC 3 cut(s) 353, 363, 392
TseI GCWGC 2 cut(s) 335, 730
Tsp45I GTSAC 3 cut(s) 353, 363, 392
TspDTI ATGAA 2 cut(s) 166, 213
TspRI CASTG 4 cut(s) 124, 372, 379, 658
VneI GTGCAC 1 cut(s) 115
XapI RAATTY 3 cut(s) 31, 341, 556
XceI RCATGY 3 cut(s) 9, 210, 420
XmnI GAANNNNTTC 1 cut(s) 505
ZrmI AGTACT 1 cut(s) 745
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.