pycom10g21440

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0015570
Physical Location & Seq
Reverse (-)
24065219 .. 24066899
1681 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom10g21440.3

Sequence Viewer

Length: 759 bp
ATGGCGTGCTTTGTGCCTTTCAACAGTAAAAATTTGGACATAAGCATGTTTGTGTTCAGGCCAACAATTGTGTTCGTCGATGACCTTCTTAATGCTCTCAAGCAATTCTCTGTGTGCACTGAGTCTCTTGGTTGTGTTCAAAGTTCCATCTTGCAGAGCATCCATGGAAATATGTTCATATGGTTTGGAGCGTGGCTGAAGAGATCATGTGAAAACAAAGACTCATTGACTGCAAGCCTTCTCTCAATGTTAACGAATTTATCGAACATGGCTATCTTACTCGATCACTCCTTCTTCGACGCGTATGCTGGAGAATCAAGTGACGGTTCTTGTGCAGCAAAATTTCGCAGAGGTGACACAGTATCACTGAGCACAGCCCTCATCAACGGCGGTGACATAAACAACGTCTCTTACGCATGCTTAGCGTTGTTCAGGTCGGGTTTTCAAAGGATGCAAGGCGTGACAGCCGGTGTTTGTTTGAAATGCCAAACCGGGCCAAGAATTGCTTCTCTCTTCGTGTGGAAGTCTCTGCACTATTGTTACTCATGGATTCTAAATTCCGACCAAAGAAAAGCAATGCTGCCCTATCTCGAAAGCGTCTCGATTGAGATTAAGTACGACATTTTTCGAGTAGTCTATGTCAGCGGCGACAGTGATATCAACCTGCAGGTCTTATATCCTCCTCATCAGATGTTAGAACCAGCTGCATGCGAAAACAAAGAAGAAGGGCAGCTCATGCAAAGTACTTCCATAGTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

253

Amino Acids

28.04

Weight (kDa)

5.79

Isoelectric Point (pI)

54.64

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015570)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g05030
malus_domestica MD05G1279400.v1.1 MD10G1258100.v1.1
prunus_persica Prupe.4G085700_v2.0.a1
pyrus_communis pycom05g25970 pycom10g21440
rosa_chinensis RchiOBHm_Chr5g0014941
rosa_laevigata RLG00000032152
rosa_multiflora Rmu_ssc0000388.1_g000067
rosa_roxburghii Rroxscaffold_1G00061640
rosa_rugosa Rorug05G0021700
rosa_samantha Rh5AG117300 Rh5BG114300 Rh5CG125800 Rh5DG113700
rosa_wichuraiana Rw5G010100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 658, 672
AccII CGCG 1 cut(s) 302
AciI CCGC 2 cut(s) 390, 645
AcsI RAATTY 4 cut(s) 31, 256, 341, 556
AcuI CTGAAG 1 cut(s) 218
AfaI GTAC 2 cut(s) 617, 745
AflIII ACRYGT 1 cut(s) 300
AgsI TTSAA 4 cut(s) 22, 140, 446, 481
AjuI GAANNNNNNNTTGG 2 cut(s) 490, 522
AluBI AGCT 2 cut(s) 704, 733
AluI AGCT 2 cut(s) 704, 733
Alw21I GWGCWC 2 cut(s) 119, 374
Alw26I GTCTC 4 cut(s) 129, 412, 531, 604
Alw44I GTGCAC 1 cut(s) 115
AoxI GGCC 2 cut(s) 59, 494
ApaLI GTGCAC 1 cut(s) 115
ApeKI GCWGC 4 cut(s) 335, 580, 704, 730
ApoI RAATTY 4 cut(s) 31, 256, 341, 556
Asp700I GAANNNNTTC 1 cut(s) 505
AspS9I GGNCC 1 cut(s) 494
AsuC2I CCSGG 1 cut(s) 493
AsuHPI GGTGA 2 cut(s) 365, 404
BaeGI GKGCMC 1 cut(s) 119
Bbv12I GWGCWC 2 cut(s) 119, 374
BbvI GCAGC 4 cut(s) 347, 567, 691, 742
BccI CCATC 1 cut(s) 155
BceAI ACGGC 1 cut(s) 403
BcgI CGANNNNNNTGC 2 cut(s) 287, 321
BcnI CCSGG 1 cut(s) 493
BcoDI GTCTC 4 cut(s) 129, 412, 531, 604
BfmI CTRYAG 1 cut(s) 665
BfuAI ACCTGC 2 cut(s) 658, 672
BisI GCNGC 5 cut(s) 336, 581, 646, 705, 731
BlpI GCTNAGC 1 cut(s) 421
BlsI GCNGC 5 cut(s) 337, 582, 647, 706, 732
BmcAI AGTACT 1 cut(s) 745
Bme1390I CCNGG 1 cut(s) 493
BmgT120I GGNCC 1 cut(s) 494
BmrFI CCNGG 1 cut(s) 493
BmsI GCATC 2 cut(s) 168, 441
BpmI CTGGAG 1 cut(s) 330
Bpu1102I GCTNAGC 1 cut(s) 421
BpuEI CTTGAG 1 cut(s) 83
BpuMI CCSGG 1 cut(s) 493
BsaJI CCNNGG 1 cut(s) 163
Bse118I RCCGGY 1 cut(s) 467
Bse3DI GCAATG 1 cut(s) 582
BseDI CCNNGG 1 cut(s) 163
BseGI GGATG 2 cut(s) 159, 456
BseMI GCAATG 1 cut(s) 582
BseMII CTCAG 2 cut(s) 111, 359
BseRI GAGGAG 1 cut(s) 672
BseSI GKGCMC 1 cut(s) 119
BseXI GCAGC 4 cut(s) 347, 567, 691, 742
BsgI GTGCAG 2 cut(s) 354, 515
Bsh1236I CGCG 1 cut(s) 302
BshFI GGCC 2 cut(s) 61, 496
BsiHKAI GWGCWC 2 cut(s) 119, 374
BsiSI CCGG 2 cut(s) 468, 492
BsmAI GTCTC 4 cut(s) 129, 412, 531, 604
BsmBI CGTCTC 2 cut(s) 412, 604
BsnI GGCC 2 cut(s) 61, 496
Bsp1286I GDGCHC 2 cut(s) 119, 374
Bsp143I GATC 2 cut(s) 203, 283
Bsp1720I GCTNAGC 1 cut(s) 421
Bsp19I CCATGG 1 cut(s) 163
BspACI CCGC 2 cut(s) 390, 645
BspANI GGCC 2 cut(s) 61, 496
BspCNI CTCAG 2 cut(s) 112, 360
BspFNI CGCG 1 cut(s) 302
BspMAI CTGCAG 1 cut(s) 669
BspMI ACCTGC 2 cut(s) 658, 672
BsrDI GCAATG 1 cut(s) 582
BsrFI RCCGGY 1 cut(s) 467
BssAI RCCGGY 1 cut(s) 467
BssECI CCNNGG 1 cut(s) 163
BssMI GATC 2 cut(s) 203, 283
BssT1I CCWWGG 1 cut(s) 163
Bst4CI ACNGT 4 cut(s) 26, 326, 361, 653
Bst6I CTCTTC 2 cut(s) 194, 518
BstAPI GCANNNNNTGC 1 cut(s) 736
BstC8I GCNNGC 4 cut(s) 7, 235, 418, 709
BstDEI CTNAG 3 cut(s) 120, 368, 421
BstDSI CCRYGG 1 cut(s) 163
BstF5I GGATG 2 cut(s) 159, 456
BstFNI CGCG 1 cut(s) 302
BstKTI GATC 2 cut(s) 206, 286
BstMAI GTCTC 4 cut(s) 129, 412, 531, 604
BstMBI GATC 2 cut(s) 203, 283
BstMWI GCNNNNNNNGC 2 cut(s) 422, 736
BstNSI RCATGY 3 cut(s) 49, 420, 711
BstSCI CCNGG 1 cut(s) 491
BstSFI CTRYAG 1 cut(s) 665
BstSLI GKGCMC 1 cut(s) 119
BstUI CGCG 1 cut(s) 302
BstV1I GCAGC 4 cut(s) 347, 567, 691, 742
BsuRI GGCC 2 cut(s) 61, 496
BtgI CCRYGG 1 cut(s) 163
BtsCI GGATG 2 cut(s) 159, 456
BtsIMutI CAGTG 3 cut(s) 117, 365, 658
BveI ACCTGC 2 cut(s) 658, 672
Cac8I GCNNGC 4 cut(s) 7, 235, 418, 709
Cfr10I RCCGGY 1 cut(s) 467
Cfr13I GGNCC 1 cut(s) 494
CseI GACGC 2 cut(s) 308, 586
Csp6I GTAC 2 cut(s) 616, 744
CviAII CATG 8 cut(s) 46, 164, 207, 268, 417, 546, 708, 736
CviJI RGCY 9 cut(s) 61, 196, 237, 272, 377, 467, 496, 704, 733
CviKI_1 RGCY 9 cut(s) 61, 196, 237, 272, 377, 467, 496, 704, 733
CviQI GTAC 2 cut(s) 616, 744
DdeI CTNAG 3 cut(s) 120, 368, 421
DpnI GATC 2 cut(s) 205, 285
DpnII GATC 2 cut(s) 203, 283
Eam1104I CTCTTC 2 cut(s) 194, 518
EarI CTCTTC 2 cut(s) 194, 518
Eco130I CCWWGG 1 cut(s) 163
Eco32I GATATC 1 cut(s) 658
Eco57I CTGAAG 1 cut(s) 218
EcoRV GATATC 1 cut(s) 658
EcoT14I CCWWGG 1 cut(s) 163
ErhI CCWWGG 1 cut(s) 163
Esp3I CGTCTC 2 cut(s) 412, 604
FaeI CATG 8 cut(s) 49, 167, 210, 271, 420, 549, 711, 739
FalI AAGNNNNNCTT 2 cut(s) 490, 522
FatI CATG 8 cut(s) 45, 163, 206, 267, 416, 545, 707, 735
FauNDI CATATG 1 cut(s) 179
Fnu4HI GCNGC 5 cut(s) 336, 581, 646, 705, 731
FokI GGATG 2 cut(s) 146, 463
Fsp4HI GCNGC 5 cut(s) 336, 581, 646, 705, 731
GluI GCNGC 5 cut(s) 336, 581, 646, 705, 731
GsuI CTGGAG 1 cut(s) 330
HaeIII GGCC 2 cut(s) 61, 496
HapII CCGG 2 cut(s) 468, 492
HgaI GACGC 2 cut(s) 308, 586
Hin1II CATG 8 cut(s) 49, 167, 210, 271, 420, 549, 711, 739
HincII GTYRAC 1 cut(s) 252
HindII GTYRAC 1 cut(s) 252
HinfI GANTC 4 cut(s) 122, 221, 314, 550
HpaI GTTAAC 1 cut(s) 252
HpaII CCGG 2 cut(s) 468, 492
HphI GGTGA 2 cut(s) 365, 404
Hpy166II GTNNAC 2 cut(s) 117, 252
Hpy188I TCNGA 2 cut(s) 562, 690
Hpy188III TCNNGA 2 cut(s) 590, 601
Hpy8I GTNNAC 2 cut(s) 117, 252
Hpy99I CGWCG 2 cut(s) 80, 302
HpyAV CCTTC 4 cut(s) 95, 248, 301, 719
HpyCH4III ACNGT 4 cut(s) 26, 326, 361, 653
HpyCH4IV ACGT 1 cut(s) 405
HpyCH4V TGCA 9 cut(s) 117, 154, 233, 335, 454, 532, 667, 707, 739
HpyF10VI GCNNNNNNNGC 2 cut(s) 422, 736
HpyF3I CTNAG 3 cut(s) 120, 368, 421
HpySE526I ACGT 1 cut(s) 405
Hsp92II CATG 8 cut(s) 49, 167, 210, 271, 420, 549, 711, 739
KspAI GTTAAC 1 cut(s) 252
Kzo9I GATC 2 cut(s) 203, 283
LmnI GCTCC 1 cut(s) 188
LpnPI CCDG 8 cut(s) 43, 294, 418, 481, 505, 653, 677, 714
Lsp1109I GCAGC 4 cut(s) 347, 567, 691, 742
LweI GCATC 2 cut(s) 168, 441
MaeII ACGT 1 cut(s) 405
MaeIII GTNAC 5 cut(s) 320, 353, 392, 460, 539
MalI GATC 2 cut(s) 205, 285
MboI GATC 2 cut(s) 203, 283
MboII GAAGA 4 cut(s) 211, 286, 505, 734
MfeI CAATTG 1 cut(s) 66
MhlI GDGCHC 2 cut(s) 119, 374
MluCI AATT 7 cut(s) 31, 66, 104, 256, 341, 501, 556
MluI ACGCGT 1 cut(s) 300
MlyI GAGTC 2 cut(s) 131, 215
MmeI TCCRAC 1 cut(s) 585
MnlI CCTC 4 cut(s) 344, 389, 690, 693
MroXI GAANNNNTTC 1 cut(s) 505
MseI TTAA 3 cut(s) 90, 251, 612
MslI CAYNNNNRTG 2 cut(s) 44, 50
MspA1I CMGCKG 2 cut(s) 645, 704
MspI CCGG 2 cut(s) 468, 492
MspR9I CCNGG 1 cut(s) 493
MunI CAATTG 1 cut(s) 66
MvnI CGCG 1 cut(s) 302
MwoI GCNNNNNNNGC 2 cut(s) 422, 736
NciI CCSGG 1 cut(s) 493
NcoI CCATGG 1 cut(s) 163
NdeI CATATG 1 cut(s) 179
NdeII GATC 2 cut(s) 203, 283
NlaIII CATG 8 cut(s) 49, 167, 210, 271, 420, 549, 711, 739
NmuCI GTSAC 4 cut(s) 320, 353, 392, 460
NspI RCATGY 3 cut(s) 49, 420, 711
PaeI GCATGC 2 cut(s) 420, 711
PcsI WCGNNNNNNNCGW 1 cut(s) 260
PdmI GAANNNNTTC 1 cut(s) 505
PfeI GAWTC 2 cut(s) 314, 550
PkrI GCNGC 5 cut(s) 337, 582, 647, 706, 732
PleI GAGTC 2 cut(s) 130, 215
PpsI GAGTC 2 cut(s) 130, 215
PspPI GGNCC 1 cut(s) 494
PstI CTGCAG 1 cut(s) 669
PvuII CAGCTG 1 cut(s) 704
RsaI GTAC 2 cut(s) 617, 745
RsaNI GTAC 2 cut(s) 616, 744
RseI CAYNNNNRTG 2 cut(s) 44, 50
SaqAI TTAA 3 cut(s) 90, 251, 612
SatI GCNGC 5 cut(s) 336, 581, 646, 705, 731
Sau3AI GATC 2 cut(s) 203, 283
Sau96I GGNCC 1 cut(s) 494
SbfI CCTGCAGG 1 cut(s) 669
ScaI AGTACT 1 cut(s) 745
SchI GAGTC 2 cut(s) 131, 215
ScrFI CCNGG 1 cut(s) 493
SdaI CCTGCAGG 1 cut(s) 669
SduI GDGCHC 2 cut(s) 119, 374
SetI ASST 8 cut(s) 87, 355, 408, 437, 666, 672, 706, 735
SfaNI GCATC 2 cut(s) 168, 441
SfcI CTRYAG 1 cut(s) 665
SmiMI CAYNNNNRTG 2 cut(s) 44, 50
SmlI CTYRAG 1 cut(s) 98
SmoI CTYRAG 1 cut(s) 98
SphI GCATGC 2 cut(s) 420, 711
Sse8387I CCTGCAGG 1 cut(s) 669
Sse9I AATT 7 cut(s) 31, 66, 104, 256, 341, 501, 556
SsiI CCGC 2 cut(s) 390, 645
StyD4I CCNGG 1 cut(s) 491
StyI CCWWGG 1 cut(s) 163
TaaI ACNGT 4 cut(s) 26, 326, 361, 653
TaiI ACGT 1 cut(s) 408
TaqI TCGA 7 cut(s) 78, 263, 282, 297, 591, 602, 628
TasI AATT 7 cut(s) 31, 66, 104, 256, 341, 501, 556
TatI WGTACW 1 cut(s) 743
TauI GCSGC 1 cut(s) 648
TfiI GAWTC 2 cut(s) 314, 550
Tru1I TTAA 3 cut(s) 90, 251, 612
Tru9I TTAA 3 cut(s) 90, 251, 612
TscAI CASTG 3 cut(s) 124, 372, 658
TseFI GTSAC 4 cut(s) 320, 353, 392, 460
TseI GCWGC 4 cut(s) 335, 580, 704, 730
Tsp45I GTSAC 4 cut(s) 320, 353, 392, 460
TspDTI ATGAA 1 cut(s) 166
TspRI CASTG 3 cut(s) 124, 372, 658
VneI GTGCAC 1 cut(s) 115
XapI RAATTY 4 cut(s) 31, 256, 341, 556
XceI RCATGY 3 cut(s) 49, 420, 711
XmnI GAANNNNTTC 1 cut(s) 505
ZrmI AGTACT 1 cut(s) 745
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.