pycom05g25970

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0015570
Physical Location & Seq
Reverse (-)
27269914 .. 27270405
492 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g25970.1

Sequence Viewer

Length: 492 bp
ATGGCTATCTTAGTCGATCACTCATTCTTCGACGCGTATGCTGGAGAATCGAGAGACGGTTCTTCCGCAGCGAAATTTCGAAGAGGTGACACGGTATCACTGAGCACTGCCCTCATCAACGGTGGTGACATAAACAACATCTCTTACGCATGCTTAGCGTTGTTCAAGTCCGGTTTTCAAAGGATGCAAGGCGCGACAGCCGGTGTTTGTTTGAAATGCCAAACGGGGCAAAGAACTGCTTCTCTCTTCGTGTGGAAGTCTCTGCAATATTGCTACTCATGGATTCTAAATTCCGACCAAAGAAAAGCATTGTCGCCCTATCTCGAAAGCGTCTCGATTGAGATTAAGTACGACATTTTTCGAGTAGTCTATGTCAGCGGCGACAGTGATATCGACTTGCAGGTCTTATATCCTCGTCATCAGATGTTAGAACAAGCTGGAGGCGAAAGCAAAGAAGAAGGGCAGCTCATGCAAAGTACTTCCATAATTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

164

Amino Acids

18.05

Weight (kDa)

5.64

Isoelectric Point (pI)

45.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7392 PF24118 8 - 120 1.9e-51 Domain of unknown function (DUF7392)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0015570)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g05030
malus_domestica MD05G1279400.v1.1 MD10G1258100.v1.1
prunus_persica Prupe.4G085700_v2.0.a1
pyrus_communis pycom05g25970 pycom10g21440
rosa_chinensis RchiOBHm_Chr5g0014941
rosa_laevigata RLG00000032152
rosa_multiflora Rmu_ssc0000388.1_g000067
rosa_roxburghii Rroxscaffold_1G00061640
rosa_rugosa Rorug05G0021700
rosa_samantha Rh5AG117300 Rh5BG114300 Rh5CG125800 Rh5DG113700
rosa_wichuraiana Rw5G010100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 401
Acc36I ACCTGC 1 cut(s) 391
AccII CGCG 2 cut(s) 35, 194
AciI CCGC 2 cut(s) 66, 378
AcsI RAATTY 2 cut(s) 74, 289
AfaI GTAC 2 cut(s) 350, 478
AflIII ACRYGT 1 cut(s) 33
AgsI TTSAA 3 cut(s) 166, 179, 214
AluBI AGCT 2 cut(s) 437, 466
AluI AGCT 2 cut(s) 437, 466
Alw21I GWGCWC 1 cut(s) 107
Alw26I GTCTC 3 cut(s) 48, 264, 337
ApeKI GCWGC 2 cut(s) 68, 463
ApoI RAATTY 2 cut(s) 74, 289
Asp700I GAANNNNTTC 1 cut(s) 238
AspLEI GCGC 1 cut(s) 194
AsuHPI GGTGA 2 cut(s) 98, 137
AsuII TTCGAA 1 cut(s) 79
Bbv12I GWGCWC 1 cut(s) 107
BbvI GCAGC 2 cut(s) 80, 475
BcgI CGANNNNNNTGC 2 cut(s) 20, 54
BcoDI GTCTC 3 cut(s) 48, 264, 337
BfuAI ACCTGC 1 cut(s) 391
BisI GCNGC 3 cut(s) 69, 379, 464
BlpI GCTNAGC 1 cut(s) 154
BlsI GCNGC 3 cut(s) 70, 380, 465
BmcAI AGTACT 1 cut(s) 478
BmsI GCATC 1 cut(s) 174
BpmI CTGGAG 2 cut(s) 63, 459
Bpu1102I GCTNAGC 1 cut(s) 154
Bpu14I TTCGAA 1 cut(s) 79
BsaWI WCCGGW 1 cut(s) 170
Bse118I RCCGGY 1 cut(s) 200
BseGI GGATG 1 cut(s) 189
BseMII CTCAG 1 cut(s) 92
BseXI GCAGC 2 cut(s) 80, 475
Bsh1236I CGCG 2 cut(s) 35, 194
BsiHKAI GWGCWC 1 cut(s) 107
BsiSI CCGG 2 cut(s) 171, 201
BsmAI GTCTC 3 cut(s) 48, 264, 337
BsmBI CGTCTC 2 cut(s) 48, 337
Bsp119I TTCGAA 1 cut(s) 79
Bsp1286I GDGCHC 1 cut(s) 107
Bsp143I GATC 1 cut(s) 16
Bsp1720I GCTNAGC 1 cut(s) 154
BspACI CCGC 2 cut(s) 66, 378
BspCNI CTCAG 1 cut(s) 93
BspFNI CGCG 2 cut(s) 35, 194
BspMI ACCTGC 1 cut(s) 391
BspT104I TTCGAA 1 cut(s) 79
BsrFI RCCGGY 1 cut(s) 200
BssAI RCCGGY 1 cut(s) 200
BssMI GATC 1 cut(s) 16
Bst4CI ACNGT 4 cut(s) 59, 94, 122, 386
Bst6I CTCTTC 2 cut(s) 76, 251
BstAPI GCANNNNNTGC 1 cut(s) 469
BstBI TTCGAA 1 cut(s) 79
BstC8I GCNNGC 1 cut(s) 151
BstDEI CTNAG 3 cut(s) 10, 101, 154
BstF5I GGATG 1 cut(s) 189
BstFNI CGCG 2 cut(s) 35, 194
BstHHI GCGC 1 cut(s) 194
BstKTI GATC 1 cut(s) 19
BstMAI GTCTC 3 cut(s) 48, 264, 337
BstMBI GATC 1 cut(s) 16
BstMWI GCNNNNNNNGC 2 cut(s) 155, 469
BstNSI RCATGY 1 cut(s) 153
BstUI CGCG 2 cut(s) 35, 194
BstV1I GCAGC 2 cut(s) 80, 475
BtsCI GGATG 1 cut(s) 189
BtsI GCAGTG 1 cut(s) 105
BtsIMutI CAGTG 3 cut(s) 98, 105, 391
BveI ACCTGC 1 cut(s) 391
Cac8I GCNNGC 1 cut(s) 151
CfoI GCGC 1 cut(s) 194
Cfr10I RCCGGY 1 cut(s) 200
CseI GACGC 2 cut(s) 41, 319
Csp6I GTAC 2 cut(s) 349, 477
CviAII CATG 3 cut(s) 150, 279, 469
CviJI RGCY 4 cut(s) 5, 200, 437, 466
CviKI_1 RGCY 4 cut(s) 5, 200, 437, 466
CviQI GTAC 2 cut(s) 349, 477
DdeI CTNAG 3 cut(s) 10, 101, 154
DpnI GATC 1 cut(s) 18
DpnII GATC 1 cut(s) 16
DrdI GACNNNNNNGTC 1 cut(s) 401
DseDI GACNNNNNNGTC 1 cut(s) 401
Eam1104I CTCTTC 2 cut(s) 76, 251
EarI CTCTTC 2 cut(s) 76, 251
Eco32I GATATC 1 cut(s) 391
EcoRV GATATC 1 cut(s) 391
Esp3I CGTCTC 2 cut(s) 48, 337
FaeI CATG 3 cut(s) 153, 282, 472
FaiI YATR 8 cut(s) 39, 131, 151, 280, 372, 409, 470, 485
FalI AAGNNNNNCTT 2 cut(s) 223, 255
FatI CATG 3 cut(s) 149, 278, 468
Fnu4HI GCNGC 3 cut(s) 69, 379, 464
FokI GGATG 1 cut(s) 196
Fsp4HI GCNGC 3 cut(s) 69, 379, 464
GlaI GCGC 1 cut(s) 193
GluI GCNGC 3 cut(s) 69, 379, 464
GsuI CTGGAG 2 cut(s) 63, 459
HapII CCGG 2 cut(s) 171, 201
HgaI GACGC 2 cut(s) 41, 319
HhaI GCGC 1 cut(s) 194
Hin1II CATG 3 cut(s) 153, 282, 472
Hin6I GCGC 1 cut(s) 192
HinP1I GCGC 1 cut(s) 192
HinfI GANTC 2 cut(s) 47, 283
HpaII CCGG 2 cut(s) 171, 201
HphI GGTGA 2 cut(s) 98, 137
Hpy188I TCNGA 2 cut(s) 295, 423
Hpy188III TCNNGA 3 cut(s) 51, 323, 334
Hpy99I CGWCG 1 cut(s) 35
HpyAV CCTTC 1 cut(s) 452
HpyCH4III ACNGT 4 cut(s) 59, 94, 122, 386
HpyCH4V TGCA 4 cut(s) 187, 265, 400, 472
HpyF10VI GCNNNNNNNGC 2 cut(s) 155, 469
HpyF3I CTNAG 3 cut(s) 10, 101, 154
Hsp92II CATG 3 cut(s) 153, 282, 472
HspAI GCGC 1 cut(s) 192
Kzo9I GATC 1 cut(s) 16
LpnPI CCDG 5 cut(s) 27, 184, 214, 386, 423
Lsp1109I GCAGC 2 cut(s) 80, 475
LweI GCATC 1 cut(s) 174
MaeIII GTNAC 2 cut(s) 86, 125
MalI GATC 1 cut(s) 18
MboI GATC 1 cut(s) 16
MboII GAAGA 5 cut(s) 19, 54, 93, 238, 467
MhlI GDGCHC 1 cut(s) 107
MluCI AATT 3 cut(s) 74, 289, 486
MluI ACGCGT 1 cut(s) 33
MmeI TCCRAC 1 cut(s) 318
MnlI CCTC 4 cut(s) 77, 122, 423, 434
MroXI GAANNNNTTC 1 cut(s) 238
MseI TTAA 2 cut(s) 345, 490
MspA1I CMGCKG 1 cut(s) 378
MspI CCGG 2 cut(s) 171, 201
MvnI CGCG 2 cut(s) 35, 194
MwoI GCNNNNNNNGC 2 cut(s) 155, 469
NdeII GATC 1 cut(s) 16
NlaIII CATG 3 cut(s) 153, 282, 472
NmuCI GTSAC 2 cut(s) 86, 125
NspI RCATGY 1 cut(s) 153
NspV TTCGAA 1 cut(s) 79
PaeI GCATGC 1 cut(s) 153
PdmI GAANNNNTTC 1 cut(s) 238
PfeI GAWTC 2 cut(s) 47, 283
PkrI GCNGC 3 cut(s) 70, 380, 465
RsaI GTAC 2 cut(s) 350, 478
RsaNI GTAC 2 cut(s) 349, 477
SaqAI TTAA 2 cut(s) 345, 490
SatI GCNGC 3 cut(s) 69, 379, 464
Sau3AI GATC 1 cut(s) 16
ScaI AGTACT 1 cut(s) 478
SduI GDGCHC 1 cut(s) 107
SetI ASST 4 cut(s) 88, 405, 439, 468
SfaNI GCATC 1 cut(s) 174
SfuI TTCGAA 1 cut(s) 79
SphI GCATGC 1 cut(s) 153
Sse9I AATT 3 cut(s) 74, 289, 486
SsiI CCGC 2 cut(s) 66, 378
SspI AATATT 1 cut(s) 269
TaaI ACNGT 4 cut(s) 59, 94, 122, 386
TaqI TCGA 8 cut(s) 15, 30, 50, 79, 324, 335, 361, 393
TasI AATT 3 cut(s) 74, 289, 486
TatI WGTACW 1 cut(s) 476
TauI GCSGC 1 cut(s) 381
TfiI GAWTC 2 cut(s) 47, 283
Tru1I TTAA 2 cut(s) 345, 490
Tru9I TTAA 2 cut(s) 345, 490
TscAI CASTG 3 cut(s) 105, 112, 391
TseFI GTSAC 2 cut(s) 86, 125
TseI GCWGC 2 cut(s) 68, 463
Tsp45I GTSAC 2 cut(s) 86, 125
TspRI CASTG 3 cut(s) 105, 112, 391
XapI RAATTY 2 cut(s) 74, 289
XceI RCATGY 1 cut(s) 153
XmnI GAANNNNTTC 1 cut(s) 238
ZrmI AGTACT 1 cut(s) 478
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.