Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr05

Orthogroup ID

OG0010057

Physical Location & Seq

Genomic Coordinates Reverse (-)

41553530 .. 41557060

Gene Structure 3531 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD05G1281600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 969 bp

ATGGTGGTGGTGTTGGGTTCTCTTAGCCAAATCAAGCCCTCTCCAATACCAACCAACTCTTCTTCTTCTTCTTCTTCCTCAAACCCCTCACGCAATTCACAGAAGAACAAAATTCTCACCAGGAGAAGAGCCATGGTGCTTGCCCCAAGCCTTGTGGCCGCTTCCTTGCTCCATTTCTGTAACCCCATTTCCCCACAACCTTCATCTCGCCTTGCACTCGCTCTGCAGGATGAGCTTCAGCAAGACGAAGACCGCGCCGTCAATCTCTTCCAGGAAACTTCTCCATCAGTTGTTTTTATTAAAGACCTTGAAATAGACAAAAGCCTCAAGGCCTCTTTTGATGTTGTCTCGCAAAGCGGAGATGAAAATTCCAAAGTTGAAGGAACAGGTTCAGGCTTCATTTGGGATAAGTTTGGTCACATTGTCACAAATTACCATGTTATAGCTAAATTGGCTACAGATCAAAGCGGATTACAGCGTTGTAAGGTTTACCTTGTGGACGCAAGAGGCAATGGTTTTTACAGTGAAGGAAAGATTGTTGGTGTCGATCCAACCTATGATTTAGCTGTTCTTAAGGTTGATGTTCAAGGACATGAGTTAAAACCTGCTGTTCTTGGTACATCTAACGATTTACATGTCGGTCAGAGCTGCTTTGCCATTGGAAATCCTTATGGATACGAGAACACACTAACAATAGGGGTGGTCAGTGGCTTAGGGAGGGAGATACCCTCACCAGATGGAAAGGCCATCAGAGGAGCTATTCAAACGGACGCTGCCATTAACTCTGGGAATTCAGGAGGGCCGTTAATTGATTCGCATGGCCATATTATCGGAGTTAACACAGCAACTTTCACTCGCAAAGGGACAGGAGCATCGTCCGGAGTTAACTTTGCAATACCGATTGACACTGTCGTGCGAACTGTGCCGTATCTTATTGTATATGGAACACCGTACCGCGACAGATTTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006091 GO:0006807 GO:0008150 GO:0008152 GO:0009507 GO:0009534 GO:0009536 GO:0009543 GO:0009579 GO:0009765 GO:0009987 GO:0010206 GO:0015979 GO:0019538 GO:0019684 GO:0030091 GO:0031976 GO:0031977 GO:0031978 GO:0031984 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044436 GO:0044444 GO:0044446 GO:0044464 GO:0071704 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Trypsin_2

Protein Analysis

323

Amino Acids

34.3

Weight (kDa)

7.05

Isoelectric Point (pI)

37.33

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Trypsin_2	PF13365	130 - 279	2.1e-31	Trypsin-like peptidase domain
Trypsin	PF00089	130 - 304	1.3e-15	Trypsin

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0010057)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G18370 AT4G18370 AT4G18370
fragaria_vesca	FvH4_3g05290 FvH4_3g05290 FvH4_3g05290 FvH4_3g05290
malus_domestica	MD05G1281600.v1.1
prunus_persica	Prupe.4G083000_v2.0.a1
pyrus_communis	pycom05g26160
rosa_chinensis	RchiOBHm_Chr5g0014431
rosa_laevigata	RLG00000032109
rosa_multiflora	Rmu_sc0031709.1_g000001 Rmu_ssc0000422.1_g000034
rosa_roxburghii	Rroxscaffold_1G00062130
rosa_rugosa	Rorug05G0018300
rosa_samantha	Rh5AG113100 Rh5BG109900 Rh5CG121600 Rh5DG108900
rosa_wichuraiana	Rw5G009810

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	257
Acc36I	ACCTGC	1 cut(s)	613
AccII	CGCG	2 cut(s)	255, 957
AccIII	TCCGGA	1 cut(s)	878
AciI	CCGC	5 cut(s)	159, 253, 357, 468, 955
AclWI	GGATC	1 cut(s)	542
AcoI	YGGCCR	2 cut(s)	156, 820
AcsI	RAATTY	3 cut(s)	111, 367, 790
AcuI	CTGAAG	1 cut(s)	221
AfaI	GTAC	2 cut(s)	619, 953
AflII	CTTAAG	1 cut(s)	572
AflIII	ACRYGT	1 cut(s)	634
AgsI	TTSAA	4 cut(s)	311, 380, 587, 764
AjnI	CCWGG	2 cut(s)	119, 270
AjuI	GAANNNNNNNTTGG	2 cut(s)	43, 75
AleI	CACNNNNGTG	1 cut(s)	911
AluBI	AGCT	5 cut(s)	235, 446, 566, 648, 758
AluI	AGCT	5 cut(s)	235, 446, 566, 648, 758
Alw26I	GTCTC	1 cut(s)	352
AlwI	GGATC	1 cut(s)	542
Aor13HI	TCCGGA	1 cut(s)	878
AoxI	GGCC	5 cut(s)	156, 330, 744, 800, 820
ApeKI	GCWGC	2 cut(s)	648, 773
ApoI	RAATTY	3 cut(s)	111, 367, 790
Asp700I	GAANNNNTTC	1 cut(s)	388
AspLEI	GCGC	1 cut(s)	257
AspS9I	GGNCC	1 cut(s)	800
AsuHPI	GGTGA	2 cut(s)	109, 723
BalI	TGGCCA	1 cut(s)	822
BbsI	GAAGAC	1 cut(s)	255
BbvI	GCAGC	2 cut(s)	635, 760
BccI	CCATC	3 cut(s)	292, 731, 755
BceAI	ACGGC	3 cut(s)	242, 787, 910
BciT130I	CCWGG	2 cut(s)	121, 272
BciVI	GTATCC	1 cut(s)	668
BcoDI	GTCTC	1 cut(s)	352
BfmI	CTRYAG	2 cut(s)	224, 456
BfrI	CTTAAG	1 cut(s)	572
BfuAI	ACCTGC	1 cut(s)	613
BfuI	GTATCC	1 cut(s)	668
BisI	GCNGC	3 cut(s)	159, 649, 774
BlsI	GCNGC	3 cut(s)	160, 650, 775
Bme1390I	CCNGG	2 cut(s)	121, 272
BmgT120I	GGNCC	1 cut(s)	800
BmrFI	CCNGG	2 cut(s)	121, 272
BmsI	GCATC	1 cut(s)	881
BpiI	GAAGAC	1 cut(s)	255
BplI	GAGNNNNNCTC	2 cut(s)	713, 745
Bpu10I	CCTNAGC	1 cut(s)	712
BpuEI	CTTGAG	1 cut(s)	311
BsaJI	CCNNGG	1 cut(s)	132
BsaWI	WCCGGW	1 cut(s)	878
Bse3DI	GCAATG	1 cut(s)	517
BseAI	TCCGGA	1 cut(s)	878
BseBI	CCWGG	2 cut(s)	121, 272
BseDI	CCNNGG	1 cut(s)	132
BseGI	GGATG	1 cut(s)	235
BseMI	GCAATG	1 cut(s)	517
BseRI	GAGGAG	1 cut(s)	768
BseXI	GCAGC	2 cut(s)	635, 760
Bsh1236I	CGCG	2 cut(s)	255, 957
BshFI	GGCC	5 cut(s)	158, 332, 746, 802, 822
BsiSI	CCGG	1 cut(s)	879
BslFI	GGGAC	1 cut(s)	877
BsmAI	GTCTC	1 cut(s)	352
BsmFI	GGGAC	1 cut(s)	877
BsnI	GGCC	5 cut(s)	158, 332, 746, 802, 822
Bsp13I	TCCGGA	1 cut(s)	878
Bsp143I	GATC	2 cut(s)	460, 547
Bsp19I	CCATGG	1 cut(s)	132
BspACI	CCGC	5 cut(s)	159, 253, 357, 468, 955
BspANI	GGCC	5 cut(s)	158, 332, 746, 802, 822
BspEI	TCCGGA	1 cut(s)	878
BspFNI	CGCG	2 cut(s)	255, 957
BspMAI	CTGCAG	1 cut(s)	228
BspMI	ACCTGC	1 cut(s)	613
BspPI	GGATC	1 cut(s)	542
BspQI	GCTCTTC	1 cut(s)	121
BspTI	CTTAAG	1 cut(s)	572
BsrDI	GCAATG	1 cut(s)	517
BssECI	CCNNGG	1 cut(s)	132
BssMI	GATC	2 cut(s)	460, 547
BssT1I	CCWWGG	1 cut(s)	132
Bst2UI	CCWGG	2 cut(s)	121, 272
Bst4CI	ACNGT	4 cut(s)	524, 910, 922, 951
Bst6I	CTCTTC	3 cut(s)	64, 121, 272
BstAFI	CTTAAG	1 cut(s)	572
BstC8I	GCNNGC	1 cut(s)	141
BstDEI	CTNAG	2 cut(s)	23, 712
BstDSI	CCRYGG	1 cut(s)	132
BstF5I	GGATG	1 cut(s)	235
BstFNI	CGCG	2 cut(s)	255, 957
BstHHI	GCGC	1 cut(s)	257
BstKTI	GATC	2 cut(s)	463, 550
BstMAI	GTCTC	1 cut(s)	352
BstMBI	GATC	2 cut(s)	460, 547
BstMWI	GCNNNNNNNGC	3 cut(s)	232, 452, 922
BstNI	CCWGG	2 cut(s)	121, 272
BstNSI	RCATGY	1 cut(s)	638
BstSCI	CCNGG	2 cut(s)	119, 270
BstSFI	CTRYAG	2 cut(s)	224, 456
BstUI	CGCG	2 cut(s)	255, 957
BstV1I	GCAGC	2 cut(s)	635, 760
BstV2I	GAAGAC	1 cut(s)	255
BsuI	GTATCC	1 cut(s)	668
BsuRI	GGCC	5 cut(s)	158, 332, 746, 802, 822
BtgI	CCRYGG	1 cut(s)	132
BtsCI	GGATG	1 cut(s)	235
BtsIMutI	CAGTG	3 cut(s)	529, 712, 906
BveI	ACCTGC	1 cut(s)	613
Cac8I	GCNNGC	1 cut(s)	141
CfoI	GCGC	1 cut(s)	257
Cfr13I	GGNCC	1 cut(s)	800
CseI	GACGC	2 cut(s)	509, 779
Csp6I	GTAC	2 cut(s)	618, 952
CspCI	CAANNNNNGTGG	4 cut(s)	135, 170, 681, 716
CviAII	CATG	5 cut(s)	133, 437, 593, 635, 818
CviQI	GTAC	2 cut(s)	618, 952
DdeI	CTNAG	2 cut(s)	23, 712
DpnI	GATC	2 cut(s)	462, 549
DpnII	GATC	2 cut(s)	460, 547
DrdI	GACNNNNNNGTC	1 cut(s)	257
DseDI	GACNNNNNNGTC	1 cut(s)	257
EaeI	YGGCCR	2 cut(s)	156, 820
Eam1104I	CTCTTC	3 cut(s)	64, 121, 272
EarI	CTCTTC	3 cut(s)	64, 121, 272
Eco130I	CCWWGG	1 cut(s)	132
Eco147I	AGGCCT	1 cut(s)	332
Eco57I	CTGAAG	1 cut(s)	221
EcoRI	GAATTC	1 cut(s)	790
EcoRII	CCWGG	2 cut(s)	119, 270
EcoT14I	CCWWGG	1 cut(s)	132
ErhI	CCWWGG	1 cut(s)	132
FaeI	CATG	5 cut(s)	136, 440, 596, 638, 821
FaqI	GGGAC	1 cut(s)	877
FatI	CATG	5 cut(s)	132, 436, 592, 634, 817
Fnu4HI	GCNGC	3 cut(s)	159, 649, 774
FokI	GGATG	1 cut(s)	242
Fsp4HI	GCNGC	3 cut(s)	159, 649, 774
GlaI	GCGC	1 cut(s)	256
GluI	GCNGC	3 cut(s)	159, 649, 774
HaeIII	GGCC	5 cut(s)	158, 332, 746, 802, 822
HapII	CCGG	1 cut(s)	879
HgaI	GACGC	2 cut(s)	509, 779
HhaI	GCGC	1 cut(s)	257
Hin1II	CATG	5 cut(s)	136, 440, 596, 638, 821
Hin6I	GCGC	1 cut(s)	255
HinP1I	GCGC	1 cut(s)	255
HincII	GTYRAC	2 cut(s)	838, 886
HindII	GTYRAC	2 cut(s)	838, 886
HinfI	GANTC	1 cut(s)	812
HpaI	GTTAAC	2 cut(s)	838, 886
HpaII	CCGG	1 cut(s)	879
HphI	GGTGA	2 cut(s)	109, 723
Hpy166II	GTNNAC	4 cut(s)	490, 499, 838, 886
Hpy188I	TCNGA	3 cut(s)	645, 752, 833
Hpy188III	TCNNGA	2 cut(s)	795, 879
Hpy8I	GTNNAC	4 cut(s)	490, 499, 838, 886
HpyAV	CCTTC	3 cut(s)	210, 374, 521
HpyCH4III	ACNGT	4 cut(s)	524, 910, 922, 951
HpyCH4V	TGCA	3 cut(s)	215, 226, 893
HpyF10VI	GCNNNNNNNGC	3 cut(s)	232, 452, 922
HpyF3I	CTNAG	2 cut(s)	23, 712
Hsp92II	CATG	5 cut(s)	136, 440, 596, 638, 821
HspAI	GCGC	1 cut(s)	255
Kpn2I	TCCGGA	1 cut(s)	878
KspAI	GTTAAC	2 cut(s)	838, 886
Kzo9I	GATC	2 cut(s)	460, 547
LguI	GCTCTTC	1 cut(s)	121
LmnI	GCTCC	3 cut(s)	174, 755, 869
Lsp1109I	GCAGC	2 cut(s)	635, 760
LweI	GCATC	1 cut(s)	881
MaeIII	GTNAC	3 cut(s)	179, 416, 424
MalI	GATC	2 cut(s)	462, 549
MboI	GATC	2 cut(s)	460, 547
MlsI	TGGCCA	1 cut(s)	822
MluCI	AATT	7 cut(s)	94, 111, 367, 430, 449, 790, 807
MluNI	TGGCCA	1 cut(s)	822
MmeI	TCCRAC	1 cut(s)	575
Mox20I	TGGCCA	1 cut(s)	822
MroI	TCCGGA	1 cut(s)	878
MroXI	GAANNNNTTC	1 cut(s)	388
MscI	TGGCCA	1 cut(s)	822
MseI	TTAA	7 cut(s)	300, 573, 599, 780, 806, 837, 885
MslI	CAYNNNNRTG	1 cut(s)	911
Msp20I	TGGCCA	1 cut(s)	822
MspCI	CTTAAG	1 cut(s)	572
MspI	CCGG	1 cut(s)	879
MspR9I	CCNGG	2 cut(s)	121, 272
MvaI	CCWGG	2 cut(s)	121, 272
MvnI	CGCG	2 cut(s)	255, 957
MwoI	GCNNNNNNNGC	3 cut(s)	232, 452, 922
NcoI	CCATGG	1 cut(s)	132
NdeII	GATC	2 cut(s)	460, 547
NlaIII	CATG	5 cut(s)	136, 440, 596, 638, 821
NmuCI	GTSAC	2 cut(s)	416, 424
NspI	RCATGY	1 cut(s)	638
OliI	CACNNNNGTG	1 cut(s)	911
PceI	AGGCCT	1 cut(s)	332
PciI	ACATGT	1 cut(s)	634
PciSI	GCTCTTC	1 cut(s)	121
PdmI	GAANNNNTTC	1 cut(s)	388
PfeI	GAWTC	1 cut(s)	812
PflFI	GACNNNGTC	1 cut(s)	908
PfoI	TCCNGGA	1 cut(s)	270
PkrI	GCNGC	3 cut(s)	160, 650, 775
PscI	ACATGT	1 cut(s)	634
Psp6I	CCWGG	2 cut(s)	119, 270
PspGI	CCWGG	2 cut(s)	119, 270
PspPI	GGNCC	1 cut(s)	800
PstI	CTGCAG	1 cut(s)	228
PsyI	GACNNNGTC	1 cut(s)	908
RsaI	GTAC	2 cut(s)	619, 953
RsaNI	GTAC	2 cut(s)	618, 952
RseI	CAYNNNNRTG	1 cut(s)	911
SapI	GCTCTTC	1 cut(s)	121
SaqAI	TTAA	7 cut(s)	300, 573, 599, 780, 806, 837, 885
SatI	GCNGC	3 cut(s)	159, 649, 774
Sau3AI	GATC	2 cut(s)	460, 547
Sau96I	GGNCC	1 cut(s)	800
ScrFI	CCNGG	2 cut(s)	121, 272
SfaNI	GCATC	1 cut(s)	881
SfcI	CTRYAG	2 cut(s)	224, 456
SmiMI	CAYNNNNRTG	1 cut(s)	911
SmlI	CTYRAG	2 cut(s)	326, 572
SmoI	CTYRAG	2 cut(s)	326, 572
Sse9I	AATT	7 cut(s)	94, 111, 367, 430, 449, 790, 807
SseBI	AGGCCT	1 cut(s)	332
SsiI	CCGC	5 cut(s)	159, 253, 357, 468, 955
StuI	AGGCCT	1 cut(s)	332
StyD4I	CCNGG	2 cut(s)	119, 270
StyI	CCWWGG	1 cut(s)	132
TaaI	ACNGT	4 cut(s)	524, 910, 922, 951
TaqI	TCGA	1 cut(s)	546
TaqII	GACCGA	1 cut(s)	629
TasI	AATT	7 cut(s)	94, 111, 367, 430, 449, 790, 807
TauI	GCSGC	1 cut(s)	161
TfiI	GAWTC	1 cut(s)	812
Tru1I	TTAA	7 cut(s)	300, 573, 599, 780, 806, 837, 885
Tru9I	TTAA	7 cut(s)	300, 573, 599, 780, 806, 837, 885
TscAI	CASTG	3 cut(s)	529, 712, 913
TseFI	GTSAC	2 cut(s)	416, 424
TseI	GCWGC	2 cut(s)	648, 773
Tsp45I	GTSAC	2 cut(s)	416, 424
TspDTI	ATGAA	3 cut(s)	192, 378, 388
TspGWI	ACGGA	1 cut(s)	782
TspRI	CASTG	3 cut(s)	529, 712, 913
Tth111I	GACNNNGTC	1 cut(s)	908
Vha464I	CTTAAG	1 cut(s)	572
XapI	RAATTY	3 cut(s)	111, 367, 790
XceI	RCATGY	1 cut(s)	638
XmnI	GAANNNNTTC	1 cut(s)	388

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.