MD05G1299600.v1.1

Protein of unknown function (DUF688)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0006667
Physical Location & Seq
Reverse (-)
43307736 .. 43309823
2088 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1299600.v1.1.491

Sequence Viewer

Length: 993 bp
ATGGAGGAAAGGAAACTGAACTTGAATGCACCTTTTCTGTCCGTCAGGCGGATTGCTACAACACCGGTGATCGCAGATAGAGAGAAAGAGAAGATAGTGCAGAGGCCCGATGGGCGGCACAGTCTTCCTCCTTATAAATCAGACTTTTGCTTGGAGCAGGTGACAGAACCGGTTGCAGTTCCTTTTCACTGGGAGCACATCCCCGGAAAAGCCAAGGAAGGCAAAGAGGCAGAGGCTACAGAGGAGGTTTCGGTTACTCCTAGGCTTCCCCCCGGAAAGCTTAGAAATGTTACAAAGCAAAGTTCGGAAAGGGATCATGGAGATCAAAATGGGGCAAGATCACCGCACTTTAATGCGTATTCTACGAAGGAAAATGTGAAGGAATTGAAGTGCTCAAAAGAGGTAATGAACCAGAGAGGAGCCTCGGAATTAGAGGACGAGGATGATATTTATTCGGATGCACTTGAGACAATTTCCCCCACGAAGTCTTTCTCCTTGAACTGTAGTGCAAGTTGTCGGAGTCAATCTGACGGTGCAGATGCAAAGTCATCTATAGCCTCCGCTGTTGATCCACAAACCCGGGACTTCTTGATGAATCGATTCTTACCTGCAGCCAAGGCAATGGCTTTGGAGACACCTCATGCTGCAAAGAAGCAACATACAGCTGCACCTGAACAGCCAAGACAAATCACGAGGGTAATCCGTGAGGATACAAGGCCCTTACCAAAGGAACAAGGGTCTCAAATGGAACTGCAACACGGACAATATGAAGAGGAAGAAGAAAGTGAAGGGGAAGATGACGAGTACGATCCCTCTGGCTCTGTTTCGGCAAAGGGTTGTGGGTTCTTTCCCCGCTTATGCTTGCGAAATTCTTTATGCCTCTTAGGTCCAATTCCAGGGATGAAAGTTAAGACTCGGGCTCTCACGTCTTCGTCCTCTGGGGTTGCAAGACCAGAAAAATCTGCTTATGCATGCTTGGGATGCTGCTTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF688

Protein Analysis

331

Amino Acids

36.44

Weight (kDa)

5.86

Isoelectric Point (pI)

54.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF688 PF05097 1 - 317 5.7e-79 Protein of unknown function (DUF688)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 135
AarI CACCTGC 1 cut(s) 148
Acc36I ACCTGC 2 cut(s) 148, 616
AciI CCGC 5 cut(s) 49, 115, 344, 561, 853
AclWI GGATC 3 cut(s) 321, 563, 803
AcsI RAATTY 1 cut(s) 868
AfaI GTAC 1 cut(s) 806
AfiI CCNNNNNNNGG 3 cut(s) 48, 114, 896
AgeI ACCGGT 2 cut(s) 64, 169
AgsI TTSAA 3 cut(s) 25, 388, 499
AjiI CACGTC 1 cut(s) 927
AjnI CCWGG 1 cut(s) 895
AluBI AGCT 2 cut(s) 280, 665
AluI AGCT 2 cut(s) 280, 665
Alw21I GWGCWC 2 cut(s) 198, 395
Alw26I GTCTC 3 cut(s) 461, 626, 744
AlwI GGATC 3 cut(s) 321, 563, 803
Ama87I CYCGRG 2 cut(s) 579, 915
AoxI GGCC 2 cut(s) 104, 716
ApeKI GCWGC 4 cut(s) 611, 644, 665, 984
ApoI RAATTY 1 cut(s) 868
AsiGI ACCGGT 2 cut(s) 64, 169
Asp700I GAANNNNTTC 2 cut(s) 488, 599
AspA2I CCTAGG 1 cut(s) 260
AspS9I GGNCC 3 cut(s) 105, 717, 887
AsuC2I CCSGG 4 cut(s) 204, 273, 580, 581
AsuHPI GGTGA 3 cut(s) 79, 172, 333
AvaI CYCGRG 2 cut(s) 579, 915
AvaII GGWCC 1 cut(s) 887
AvrII CCTAGG 1 cut(s) 260
BanII GRGCYC 1 cut(s) 922
BauI CACGAG 1 cut(s) 691
BbsI GAAGAC 2 cut(s) 116, 921
Bbv12I GWGCWC 2 cut(s) 198, 395
BbvI GCAGC 4 cut(s) 623, 631, 652, 971
BccI CCATC 1 cut(s) 104
BciT130I CCWGG 1 cut(s) 897
BciVI GTATCC 1 cut(s) 703
BcnI CCSGG 4 cut(s) 204, 273, 580, 581
BcoDI GTCTC 3 cut(s) 461, 626, 744
BfaI CTAG 1 cut(s) 261
BfmI CTRYAG 4 cut(s) 237, 502, 552, 609
BfuAI ACCTGC 2 cut(s) 148, 616
BfuI GTATCC 1 cut(s) 703
BglI GCCNNNNNGGC 1 cut(s) 112
BisI GCNGC 5 cut(s) 116, 612, 645, 666, 985
BlnI CCTAGG 1 cut(s) 260
BlsI GCNGC 5 cut(s) 117, 613, 646, 667, 986
Bme1390I CCNGG 5 cut(s) 204, 273, 580, 581, 897
Bme18I GGWCC 1 cut(s) 887
BmeT110I CYCGRG 2 cut(s) 579, 915
BmgBI CACGTC 1 cut(s) 927
BmgT120I GGNCC 3 cut(s) 105, 717, 887
BmiI GGNNCC 1 cut(s) 421
BmrFI CCNGG 5 cut(s) 204, 273, 580, 581, 897
BmrI ACTGGG 1 cut(s) 199
BmsI GCATC 3 cut(s) 448, 529, 971
BmuI ACTGGG 1 cut(s) 199
BpiI GAAGAC 2 cut(s) 116, 921
BpuEI CTTGAG 1 cut(s) 485
BpuMI CCSGG 4 cut(s) 204, 273, 580, 581
Bsa29I ATCGAT 1 cut(s) 598
BsaI GGTCTC 1 cut(s) 744
BsaJI CCNNGG 8 cut(s) 202, 213, 260, 271, 423, 579, 615, 896
BsaWI WCCGGW 2 cut(s) 64, 169
Bsc4I CCNNNNNNNGG 3 cut(s) 48, 114, 896
Bse118I RCCGGY 2 cut(s) 64, 169
Bse1I ACTGG 1 cut(s) 194
Bse3DI GCAATG 1 cut(s) 627
BseBI CCWGG 1 cut(s) 897
BseCI ATCGAT 1 cut(s) 598
BseDI CCNNGG 8 cut(s) 202, 213, 260, 271, 423, 579, 615, 896
BseGI GGATG 5 cut(s) 198, 448, 463, 906, 986
BseLI CCNNNNNNNGG 3 cut(s) 48, 114, 896
BseMI GCAATG 1 cut(s) 627
BseNI ACTGG 1 cut(s) 194
BseRI GAGGAG 2 cut(s) 257, 432
BseXI GCAGC 4 cut(s) 623, 631, 652, 971
BsgI GTGCAG 3 cut(s) 119, 555, 651
BshFI GGCC 2 cut(s) 106, 718
BshTI ACCGGT 2 cut(s) 64, 169
BshVI ATCGAT 1 cut(s) 598
BsiHKAI GWGCWC 2 cut(s) 198, 395
BsiHKCI CYCGRG 2 cut(s) 579, 915
BsiSI CCGG 5 cut(s) 65, 170, 204, 273, 580
BslFI GGGAC 1 cut(s) 596
BslI CCNNNNNNNGG 3 cut(s) 48, 114, 896
BsmAI GTCTC 3 cut(s) 461, 626, 744
BsmFI GGGAC 1 cut(s) 596
BsmI GAATGC 1 cut(s) 31
BsnI GGCC 2 cut(s) 106, 718
Bso31I GGTCTC 1 cut(s) 744
BsoBI CYCGRG 2 cut(s) 579, 915
Bsp1286I GDGCHC 3 cut(s) 198, 395, 922
Bsp143I GATC 6 cut(s) 69, 313, 322, 338, 568, 808
BspACI CCGC 5 cut(s) 49, 115, 344, 561, 853
BspANI GGCC 2 cut(s) 106, 718
BspDI ATCGAT 1 cut(s) 598
BspLI GGNNCC 1 cut(s) 421
BspMAI CTGCAG 1 cut(s) 613
BspMI ACCTGC 2 cut(s) 148, 616
BspPI GGATC 3 cut(s) 321, 563, 803
BspTNI GGTCTC 1 cut(s) 744
BsrDI GCAATG 1 cut(s) 627
BsrFI RCCGGY 2 cut(s) 64, 169
BsrI ACTGG 1 cut(s) 194
BssAI RCCGGY 2 cut(s) 64, 169
BssECI CCNNGG 8 cut(s) 202, 213, 260, 271, 423, 579, 615, 896
BssMI GATC 6 cut(s) 69, 313, 322, 338, 568, 808
BssSI CACGAG 1 cut(s) 691
BssT1I CCWWGG 3 cut(s) 213, 260, 615
Bst2BI CACGAG 1 cut(s) 691
Bst2UI CCWGG 1 cut(s) 897
Bst4CI ACNGT 3 cut(s) 122, 503, 533
Bst6I CTCTTC 1 cut(s) 765
BstC8I GCNNGC 2 cut(s) 863, 973
BstDEI CTNAG 2 cut(s) 281, 883
BstF5I GGATG 5 cut(s) 198, 448, 463, 906, 986
BstKTI GATC 6 cut(s) 72, 316, 325, 341, 571, 811
BstMAI GTCTC 3 cut(s) 461, 626, 744
BstMBI GATC 6 cut(s) 69, 313, 322, 338, 568, 808
BstMWI GCNNNNNNNGC 3 cut(s) 112, 617, 981
BstNI CCWGG 1 cut(s) 897
BstNSI RCATGY 1 cut(s) 975
BstSCI CCNGG 5 cut(s) 202, 271, 578, 579, 895
BstSFI CTRYAG 4 cut(s) 237, 502, 552, 609
BstV1I GCAGC 4 cut(s) 623, 631, 652, 971
BstV2I GAAGAC 2 cut(s) 116, 921
BstXI CCANNNNNNTGG 1 cut(s) 622
Bsu15I ATCGAT 1 cut(s) 598
BsuI GTATCC 1 cut(s) 703
BsuRI GGCC 2 cut(s) 106, 718
BsuTUI ATCGAT 1 cut(s) 598
BtrI CACGTC 1 cut(s) 927
BtsCI GGATG 5 cut(s) 198, 448, 463, 906, 986
BtsIMutI CAGTG 1 cut(s) 187
BveI ACCTGC 2 cut(s) 148, 616
Cac8I GCNNGC 2 cut(s) 863, 973
Cfr10I RCCGGY 2 cut(s) 64, 169
Cfr13I GGNCC 3 cut(s) 105, 717, 887
Cfr9I CCCGGG 1 cut(s) 579
ClaI ATCGAT 1 cut(s) 598
Csp6I GTAC 1 cut(s) 805
CspAI ACCGGT 2 cut(s) 64, 169
CviAII CATG 3 cut(s) 317, 641, 972
CviQI GTAC 1 cut(s) 805
DdeI CTNAG 2 cut(s) 281, 883
DpnI GATC 6 cut(s) 71, 315, 324, 340, 570, 810
DpnII GATC 6 cut(s) 69, 313, 322, 338, 568, 808
Eam1104I CTCTTC 1 cut(s) 765
EarI CTCTTC 1 cut(s) 765
EciI GGCGGA 1 cut(s) 64
Eco130I CCWWGG 3 cut(s) 213, 260, 615
Eco24I GRGCYC 1 cut(s) 922
Eco31I GGTCTC 1 cut(s) 744
Eco47I GGWCC 1 cut(s) 887
Eco88I CYCGRG 2 cut(s) 579, 915
EcoO109I RGGNCCY 1 cut(s) 717
EcoRII CCWGG 1 cut(s) 895
EcoT14I CCWWGG 3 cut(s) 213, 260, 615
EcoT22I ATGCAT 1 cut(s) 973
EcoT38I GRGCYC 1 cut(s) 922
ErhI CCWWGG 3 cut(s) 213, 260, 615
FaeI CATG 3 cut(s) 320, 644, 975
FaqI GGGAC 1 cut(s) 596
FatI CATG 3 cut(s) 316, 640, 971
FauI CCCGC 1 cut(s) 860
Fnu4HI GCNGC 5 cut(s) 116, 612, 645, 666, 985
FokI GGATG 4 cut(s) 185, 455, 470, 913
FriOI GRGCYC 1 cut(s) 922
Fsp4HI GCNGC 5 cut(s) 116, 612, 645, 666, 985
FspBI CTAG 1 cut(s) 261
GluI GCNGC 5 cut(s) 116, 612, 645, 666, 985
HaeIII GGCC 2 cut(s) 106, 718
HapII CCGG 5 cut(s) 65, 170, 204, 273, 580
Hin1II CATG 3 cut(s) 320, 644, 975
HindIII AAGCTT 1 cut(s) 278
HinfI GANTC 4 cut(s) 520, 595, 600, 913
HpaII CCGG 5 cut(s) 65, 170, 204, 273, 580
HphI GGTGA 3 cut(s) 79, 172, 333
Hpy188I TCNGA 6 cut(s) 142, 307, 427, 457, 519, 529
Hpy188III TCNNGA 2 cut(s) 589, 691
HpyAV CCTTC 4 cut(s) 212, 361, 373, 782
HpyCH4III ACNGT 3 cut(s) 122, 503, 533
HpyCH4IV ACGT 1 cut(s) 926
HpyF10VI GCNNNNNNNGC 3 cut(s) 112, 617, 981
HpyF3I CTNAG 2 cut(s) 281, 883
HpySE526I ACGT 1 cut(s) 926
Hsp92II CATG 3 cut(s) 320, 644, 975
Kzo9I GATC 6 cut(s) 69, 313, 322, 338, 568, 808
LmnI GCTCC 3 cut(s) 154, 193, 419
Lsp1109I GCAGC 4 cut(s) 623, 631, 652, 971
LweI GCATC 3 cut(s) 448, 529, 971
MaeI CTAG 1 cut(s) 261
MaeII ACGT 1 cut(s) 926
MaeIII GTNAC 3 cut(s) 160, 253, 289
MalI GATC 6 cut(s) 71, 315, 324, 340, 570, 810
MboI GATC 6 cut(s) 69, 313, 322, 338, 568, 808
MboII GAAGA 7 cut(s) 103, 116, 782, 788, 791, 806, 921
MhlI GDGCHC 3 cut(s) 198, 395, 922
MluCI AATT 5 cut(s) 383, 428, 471, 868, 891
MlyI GAGTC 2 cut(s) 529, 907
MmeI TCCRAC 1 cut(s) 497
Mph1103I ATGCAT 1 cut(s) 973
MroXI GAANNNNTTC 2 cut(s) 488, 599
MseI TTAA 2 cut(s) 351, 909
MslI CAYNNNNRTG 1 cut(s) 351
MspA1I CMGCKG 2 cut(s) 563, 665
MspI CCGG 5 cut(s) 65, 170, 204, 273, 580
MspR9I CCNGG 5 cut(s) 204, 273, 580, 581, 897
Mva1269I GAATGC 1 cut(s) 31
MvaI CCWGG 1 cut(s) 897
MwoI GCNNNNNNNGC 3 cut(s) 112, 617, 981
NciI CCSGG 4 cut(s) 204, 273, 580, 581
NdeII GATC 6 cut(s) 69, 313, 322, 338, 568, 808
NlaIII CATG 3 cut(s) 320, 644, 975
NlaIV GGNNCC 1 cut(s) 421
NmuCI GTSAC 1 cut(s) 160
NsiI ATGCAT 1 cut(s) 973
NspI RCATGY 1 cut(s) 975
PaeI GCATGC 1 cut(s) 975
PaqCI CACCTGC 1 cut(s) 148
PctI GAATGC 1 cut(s) 31
PdmI GAANNNNTTC 2 cut(s) 488, 599
PfeI GAWTC 2 cut(s) 595, 600
PinAI ACCGGT 2 cut(s) 64, 169
PkrI GCNGC 5 cut(s) 117, 613, 646, 667, 986
PleI GAGTC 2 cut(s) 528, 907
PpsI GAGTC 2 cut(s) 528, 907
PsiI TTATAA 1 cut(s) 135
Psp6I CCWGG 1 cut(s) 895
PspGI CCWGG 1 cut(s) 895
PspN4I GGNNCC 1 cut(s) 421
PspPI GGNCC 3 cut(s) 105, 717, 887
PstI CTGCAG 1 cut(s) 613
PvuII CAGCTG 1 cut(s) 665
RsaI GTAC 1 cut(s) 806
RsaNI GTAC 1 cut(s) 805
RseI CAYNNNNRTG 1 cut(s) 351
SaqAI TTAA 2 cut(s) 351, 909
SatI GCNGC 5 cut(s) 116, 612, 645, 666, 985
Sau3AI GATC 6 cut(s) 69, 313, 322, 338, 568, 808
Sau96I GGNCC 3 cut(s) 105, 717, 887
SchI GAGTC 2 cut(s) 529, 907
ScrFI CCNGG 5 cut(s) 204, 273, 580, 581, 897
SduI GDGCHC 3 cut(s) 198, 395, 922
SfaNI GCATC 3 cut(s) 448, 529, 971
SfcI CTRYAG 4 cut(s) 237, 502, 552, 609
SgrAI CRCCGGYG 1 cut(s) 64
SinI GGWCC 1 cut(s) 887
SmaI CCCGGG 1 cut(s) 581
SmiMI CAYNNNNRTG 1 cut(s) 351
SmlI CTYRAG 1 cut(s) 464
SmoI CTYRAG 1 cut(s) 464
SphI GCATGC 1 cut(s) 975
Sse9I AATT 5 cut(s) 383, 428, 471, 868, 891
SsiI CCGC 5 cut(s) 49, 115, 344, 561, 853
SspMI CTAG 1 cut(s) 261
StyD4I CCNGG 5 cut(s) 202, 271, 578, 579, 895
StyI CCWWGG 3 cut(s) 213, 260, 615
TaaI ACNGT 3 cut(s) 122, 503, 533
TaiI ACGT 1 cut(s) 929
TaqI TCGA 1 cut(s) 598
TasI AATT 5 cut(s) 383, 428, 471, 868, 891
TauI GCSGC 1 cut(s) 118
TfiI GAWTC 2 cut(s) 595, 600
Tru1I TTAA 2 cut(s) 351, 909
Tru9I TTAA 2 cut(s) 351, 909
TscAI CASTG 1 cut(s) 194
TseFI GTSAC 1 cut(s) 160
TseI GCWGC 4 cut(s) 611, 644, 665, 984
Tsp45I GTSAC 1 cut(s) 160
TspDTI ATGAA 4 cut(s) 422, 608, 783, 917
TspGWI ACGGA 3 cut(s) 31, 692, 774
TspMI CCCGGG 1 cut(s) 579
TspRI CASTG 1 cut(s) 194
VpaK11BI GGWCC 1 cut(s) 887
XapI RAATTY 1 cut(s) 868
XceI RCATGY 1 cut(s) 975
XmaI CCCGGG 1 cut(s) 579
XmaJI CCTAGG 1 cut(s) 260
XmnI GAANNNNTTC 2 cut(s) 488, 599
XspI CTAG 1 cut(s) 261
Zsp2I ATGCAT 1 cut(s) 973
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.