pycom10g23290

Protein of unknown function (DUF688)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0006667
Physical Location & Seq
Reverse (-)
25487202 .. 25488403
1202 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom10g23290.1

Sequence Viewer

Length: 1107 bp
ATGGAGGAAAGGAAACTGAATTTGAATGCGCCTTTTCTGTCCGTGAGACGAATTGCTACAACACCAGTGATCGCAGATGGAGAGAAAGAGAAGATAATGCAGAGGCCCGATGGGCGGCATACTCTTCCTTCTTACAAATCGGACTTTCGTTTGGAGCAGGTGACAGAACCGGTTGCAGTTCCTTTTCACTGGGAGCACATCCCCGGAAAAGCCAAGGAAGACAATGAGGCAGAGCTTCAGCCTACAGAGGAGGCTTCAGTTACTCCTAGGCTTCCCCGGGGAAAGCTTATAGATGTTACAAAACATAATTCAGAAAGGGACTATGGAGATCATAAAGCGTATTCTTTGAAAGAAAATGTGAAGGAGTTGAAGTGCTCGAAAGATGTAACAAACGAGAGAATGGCCTTGGAATTACAGAATGAGGATGATGTTTATTCGGATGCACTTGAGACGATTTCCCCTACTAAGTCTTTCTCATTGAACTGTAGTGCAAGTTGTCTCAGTCAATCTGAAAGTGCAGACGTGAAGCCATCTAGACCCTCCGCTATTGATCCACAAGCCCGGGATTTCATGATGAATCGATTCTTACCTGCAGCCAAGGCAATGTCTTTGGAGACACCTCACGCTGCAAAGAAGCAGCATACAGCTGCACCTGAACCGCCAAGAAGAATCACGAGGGTAATCCGCCGTGAGGATACGAGGCCCTTACCTAAGGAACAGGTATCTCAAATGGAACTGCAACACGGACAATACGAAGAGGAAAAAGAAAGTGAAGGAGAAGATGACGATTACGATCCCTCTGGCTCTGTTTCAGCGAAGGGTTGTGGGTTGTTTCCCCGGTTGTGCTTGCGAAACTCTTTATGCCTCTTAGGTCCAATTCCAGGGATGAAAGTTAAGACCCGGGGTCTCATGTCTTCGTCCTCTAAGGTTGCAAGACCAGAAAAATCTACTTATGGTCGGTCTCACAGCCAACAACTCAACAAGCATGCTTGGGATGCTGCTTATAAGCGCAAATCAAACTGTGGAGTCCAATCAGGTGAATTGCTGAAGTTGGAGAATAAGAAGGCTGGTGAGTCCAATCGCTTTACTCATTCTGGATTGATCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF688

Protein Analysis

369

Amino Acids

41.29

Weight (kDa)

8.07

Isoelectric Point (pI)

53.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF688 PF05097 1 - 362 6.1e-81 Protein of unknown function (DUF688)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1005
AarI CACCTGC 1 cut(s) 148
Acc36I ACCTGC 2 cut(s) 148, 598
AciI CCGC 4 cut(s) 115, 543, 659, 685
AclWI GGATC 2 cut(s) 545, 788
AcsI RAATTY 1 cut(s) 19
AcuI CTGAAG 3 cut(s) 221, 240, 1067
AfiI CCNNNNNNNGG 3 cut(s) 114, 691, 881
AgeI ACCGGT 1 cut(s) 169
AgsI TTSAA 4 cut(s) 25, 349, 370, 481
AhdI GACNNNNNGTC 1 cut(s) 903
AjiI CACGTC 1 cut(s) 523
AjnI CCWGG 1 cut(s) 880
AluBI AGCT 3 cut(s) 235, 286, 647
AluI AGCT 3 cut(s) 235, 286, 647
Alw21I GWGCWC 2 cut(s) 198, 377
Alw26I GTCTC 6 cut(s) 40, 443, 503, 608, 911, 966
AlwI GGATC 2 cut(s) 545, 788
Ama87I CYCGRG 3 cut(s) 276, 561, 900
AoxI GGCC 3 cut(s) 104, 402, 701
ApeKI GCWGC 5 cut(s) 593, 626, 637, 647, 998
ApoI RAATTY 1 cut(s) 19
AsiGI ACCGGT 1 cut(s) 169
Asp700I GAANNNNTTC 1 cut(s) 581
AspA2I CCTAGG 1 cut(s) 266
AspLEI GCGC 2 cut(s) 31, 1011
AspS9I GGNCC 3 cut(s) 105, 702, 872
AsuC2I CCSGG 8 cut(s) 204, 277, 278, 562, 563, 838, 901, 902
AsuHPI GGTGA 3 cut(s) 172, 1049, 1082
AvaI CYCGRG 3 cut(s) 276, 561, 900
AvaII GGWCC 1 cut(s) 872
AvrII CCTAGG 1 cut(s) 266
AxyI CCTNAGG 1 cut(s) 711
BauI CACGAG 1 cut(s) 673
BbsI GAAGAC 2 cut(s) 225, 906
Bbv12I GWGCWC 2 cut(s) 198, 377
BbvI GCAGC 5 cut(s) 605, 613, 634, 649, 985
BccI CCATC 3 cut(s) 71, 104, 538
BceAI ACGGC 1 cut(s) 672
BciT130I CCWGG 1 cut(s) 882
BciVI GTATCC 1 cut(s) 688
BcnI CCSGG 8 cut(s) 204, 277, 278, 562, 563, 838, 901, 902
BcoDI GTCTC 6 cut(s) 40, 443, 503, 608, 911, 966
BfaI CTAG 2 cut(s) 267, 534
BfmI CTRYAG 3 cut(s) 243, 484, 591
BfuAI ACCTGC 2 cut(s) 148, 598
BfuI GTATCC 1 cut(s) 688
BglI GCCNNNNNGGC 1 cut(s) 112
BisI GCNGC 6 cut(s) 116, 594, 627, 638, 648, 999
BlnI CCTAGG 1 cut(s) 266
BlsI GCNGC 6 cut(s) 117, 595, 628, 639, 649, 1000
Bme1390I CCNGG 9 cut(s) 204, 277, 278, 562, 563, 838, 882, 901, 902
Bme18I GGWCC 1 cut(s) 872
BmeRI GACNNNNNGTC 1 cut(s) 903
BmeT110I CYCGRG 3 cut(s) 276, 561, 900
BmgBI CACGTC 1 cut(s) 523
BmgT120I GGNCC 3 cut(s) 105, 702, 872
BmrFI CCNGG 9 cut(s) 204, 277, 278, 562, 563, 838, 882, 901, 902
BmrI ACTGGG 1 cut(s) 199
BmsI GCATC 2 cut(s) 430, 985
BmuI ACTGGG 1 cut(s) 199
BpiI GAAGAC 2 cut(s) 225, 906
BpuEI CTTGAG 1 cut(s) 467
BpuMI CCSGG 8 cut(s) 204, 277, 278, 562, 563, 838, 901, 902
Bsa29I ATCGAT 1 cut(s) 580
BsaBI GATNNNNATC 1 cut(s) 792
BsaI GGTCTC 2 cut(s) 911, 966
BsaWI WCCGGW 1 cut(s) 169
BsaXI ACNNNNNCTCC 2 cut(s) 356, 386
Bsc4I CCNNNNNNNGG 3 cut(s) 114, 691, 881
Bse118I RCCGGY 1 cut(s) 169
Bse1I ACTGG 2 cut(s) 65, 194
Bse21I CCTNAGG 1 cut(s) 711
Bse3DI GCAATG 1 cut(s) 609
Bse8I GATNNNNATC 1 cut(s) 792
BseBI CCWGG 1 cut(s) 882
BseCI ATCGAT 1 cut(s) 580
BseGI GGATG 5 cut(s) 198, 430, 445, 891, 1000
BseJI GATNNNNATC 1 cut(s) 792
BseLI CCNNNNNNNGG 3 cut(s) 114, 691, 881
BseMI GCAATG 1 cut(s) 609
BseMII CTCAG 1 cut(s) 514
BseNI ACTGG 2 cut(s) 65, 194
BseRI GAGGAG 1 cut(s) 263
BseXI GCAGC 5 cut(s) 605, 613, 634, 649, 985
BsgI GTGCAG 2 cut(s) 537, 633
BshFI GGCC 3 cut(s) 106, 404, 703
BshTI ACCGGT 1 cut(s) 169
BshVI ATCGAT 1 cut(s) 580
BsiHKAI GWGCWC 2 cut(s) 198, 377
BsiHKCI CYCGRG 3 cut(s) 276, 561, 900
BsiSI CCGG 6 cut(s) 170, 204, 277, 562, 838, 901
BslFI GGGAC 1 cut(s) 332
BslI CCNNNNNNNGG 3 cut(s) 114, 691, 881
BsmAI GTCTC 6 cut(s) 40, 443, 503, 608, 911, 966
BsmBI CGTCTC 2 cut(s) 40, 443
BsmFI GGGAC 1 cut(s) 332
BsmI GAATGC 1 cut(s) 31
BsnI GGCC 3 cut(s) 106, 404, 703
Bso31I GGTCTC 2 cut(s) 911, 966
BsoBI CYCGRG 3 cut(s) 276, 561, 900
Bsp1286I GDGCHC 2 cut(s) 198, 377
Bsp143I GATC 5 cut(s) 69, 328, 550, 793, 1101
BspACI CCGC 4 cut(s) 115, 543, 659, 685
BspANI GGCC 3 cut(s) 106, 404, 703
BspCNI CTCAG 1 cut(s) 513
BspDI ATCGAT 1 cut(s) 580
BspHI TCATGA 1 cut(s) 570
BspMAI CTGCAG 1 cut(s) 595
BspMI ACCTGC 2 cut(s) 148, 598
BspPI GGATC 2 cut(s) 545, 788
BspTNI GGTCTC 2 cut(s) 911, 966
BsrDI GCAATG 1 cut(s) 609
BsrFI RCCGGY 1 cut(s) 169
BsrI ACTGG 2 cut(s) 65, 194
BssAI RCCGGY 1 cut(s) 169
BssMI GATC 5 cut(s) 69, 328, 550, 793, 1101
BssSI CACGAG 1 cut(s) 673
BssT1I CCWWGG 4 cut(s) 213, 266, 405, 597
Bst2BI CACGAG 1 cut(s) 673
Bst2UI CCWGG 1 cut(s) 882
Bst4CI ACNGT 2 cut(s) 485, 1022
Bst6I CTCTTC 2 cut(s) 129, 750
BstC8I GCNNGC 2 cut(s) 848, 987
BstDEI CTNAG 5 cut(s) 465, 500, 711, 868, 924
BstF5I GGATG 5 cut(s) 198, 430, 445, 891, 1000
BstHHI GCGC 2 cut(s) 31, 1011
BstKTI GATC 5 cut(s) 72, 331, 553, 796, 1104
BstMAI GTCTC 6 cut(s) 40, 443, 503, 608, 911, 966
BstMBI GATC 5 cut(s) 69, 328, 550, 793, 1101
BstMWI GCNNNNNNNGC 3 cut(s) 112, 599, 995
BstNI CCWGG 1 cut(s) 882
BstNSI RCATGY 1 cut(s) 989
BstSCI CCNGG 9 cut(s) 202, 275, 276, 560, 561, 836, 880, 899, 900
BstSFI CTRYAG 3 cut(s) 243, 484, 591
BstV1I GCAGC 5 cut(s) 605, 613, 634, 649, 985
BstV2I GAAGAC 2 cut(s) 225, 906
Bsu15I ATCGAT 1 cut(s) 580
Bsu36I CCTNAGG 1 cut(s) 711
BsuI GTATCC 1 cut(s) 688
BsuRI GGCC 3 cut(s) 106, 404, 703
BsuTUI ATCGAT 1 cut(s) 580
BtrI CACGTC 1 cut(s) 523
BtsCI GGATG 5 cut(s) 198, 430, 445, 891, 1000
BtsIMutI CAGTG 2 cut(s) 72, 187
BveI ACCTGC 2 cut(s) 148, 598
Cac8I GCNNGC 2 cut(s) 848, 987
CciI TCATGA 1 cut(s) 570
CfoI GCGC 2 cut(s) 31, 1011
Cfr10I RCCGGY 1 cut(s) 169
Cfr13I GGNCC 3 cut(s) 105, 702, 872
Cfr9I CCCGGG 3 cut(s) 276, 561, 900
ClaI ATCGAT 1 cut(s) 580
CspAI ACCGGT 1 cut(s) 169
CviAII CATG 3 cut(s) 571, 910, 986
DdeI CTNAG 5 cut(s) 465, 500, 711, 868, 924
DpnI GATC 5 cut(s) 71, 330, 552, 795, 1103
DpnII GATC 5 cut(s) 69, 328, 550, 793, 1101
DriI GACNNNNNGTC 1 cut(s) 903
Eam1104I CTCTTC 2 cut(s) 129, 750
Eam1105I GACNNNNNGTC 1 cut(s) 903
EarI CTCTTC 2 cut(s) 129, 750
EciI GGCGGA 1 cut(s) 674
Eco130I CCWWGG 4 cut(s) 213, 266, 405, 597
Eco31I GGTCTC 2 cut(s) 911, 966
Eco47I GGWCC 1 cut(s) 872
Eco57I CTGAAG 3 cut(s) 221, 240, 1067
Eco81I CCTNAGG 1 cut(s) 711
Eco88I CYCGRG 3 cut(s) 276, 561, 900
EcoO109I RGGNCCY 1 cut(s) 702
EcoRII CCWGG 1 cut(s) 880
EcoT14I CCWWGG 4 cut(s) 213, 266, 405, 597
ErhI CCWWGG 4 cut(s) 213, 266, 405, 597
Esp3I CGTCTC 2 cut(s) 40, 443
FaeI CATG 3 cut(s) 574, 913, 989
FaqI GGGAC 1 cut(s) 332
FatI CATG 3 cut(s) 570, 909, 985
Fnu4HI GCNGC 6 cut(s) 116, 594, 627, 638, 648, 999
FokI GGATG 5 cut(s) 185, 437, 452, 898, 1007
Fsp4HI GCNGC 6 cut(s) 116, 594, 627, 638, 648, 999
FspBI CTAG 2 cut(s) 267, 534
GlaI GCGC 2 cut(s) 30, 1010
GluI GCNGC 6 cut(s) 116, 594, 627, 638, 648, 999
HaeIII GGCC 3 cut(s) 106, 404, 703
HapII CCGG 6 cut(s) 170, 204, 277, 562, 838, 901
HhaI GCGC 2 cut(s) 31, 1011
Hin1II CATG 3 cut(s) 574, 913, 989
Hin6I GCGC 2 cut(s) 29, 1009
HinP1I GCGC 2 cut(s) 29, 1009
HindIII AAGCTT 1 cut(s) 284
HinfI GANTC 5 cut(s) 577, 582, 669, 1026, 1073
HpaII CCGG 6 cut(s) 170, 204, 277, 562, 838, 901
HphI GGTGA 3 cut(s) 172, 1049, 1082
Hpy188I TCNGA 4 cut(s) 142, 313, 439, 511
Hpy188III TCNNGA 4 cut(s) 534, 571, 673, 1095
HpyAV CCTTC 5 cut(s) 138, 355, 767, 811, 1057
HpyCH4III ACNGT 2 cut(s) 485, 1022
HpyCH4IV ACGT 1 cut(s) 522
HpyF10VI GCNNNNNNNGC 3 cut(s) 112, 599, 995
HpyF3I CTNAG 5 cut(s) 465, 500, 711, 868, 924
HpySE526I ACGT 1 cut(s) 522
Hsp92II CATG 3 cut(s) 574, 913, 989
HspAI GCGC 2 cut(s) 29, 1009
Kzo9I GATC 5 cut(s) 69, 328, 550, 793, 1101
LmnI GCTCC 2 cut(s) 154, 193
Lsp1109I GCAGC 5 cut(s) 605, 613, 634, 649, 985
LweI GCATC 2 cut(s) 430, 985
MaeI CTAG 2 cut(s) 267, 534
MaeII ACGT 1 cut(s) 522
MaeIII GTNAC 4 cut(s) 160, 259, 295, 385
MalI GATC 5 cut(s) 71, 330, 552, 795, 1103
MboI GATC 5 cut(s) 69, 328, 550, 793, 1101
MboII GAAGA 7 cut(s) 103, 116, 230, 678, 767, 791, 906
MhlI GDGCHC 2 cut(s) 198, 377
MluCI AATT 6 cut(s) 19, 51, 307, 410, 876, 1040
MlyI GAGTC 2 cut(s) 1035, 1082
MmeI TCCRAC 1 cut(s) 1032
MroXI GAANNNNTTC 1 cut(s) 581
MseI TTAA 1 cut(s) 894
MspA1I CMGCKG 1 cut(s) 647
MspI CCGG 6 cut(s) 170, 204, 277, 562, 838, 901
MspR9I CCNGG 9 cut(s) 204, 277, 278, 562, 563, 838, 882, 901, 902
Mva1269I GAATGC 1 cut(s) 31
MvaI CCWGG 1 cut(s) 882
MwoI GCNNNNNNNGC 3 cut(s) 112, 599, 995
NciI CCSGG 8 cut(s) 204, 277, 278, 562, 563, 838, 901, 902
NdeII GATC 5 cut(s) 69, 328, 550, 793, 1101
NlaIII CATG 3 cut(s) 574, 913, 989
NmuCI GTSAC 1 cut(s) 160
NspI RCATGY 1 cut(s) 989
PaeI GCATGC 1 cut(s) 989
PagI TCATGA 1 cut(s) 570
PaqCI CACCTGC 1 cut(s) 148
PcsI WCGNNNNNNNCGW 1 cut(s) 750
PctI GAATGC 1 cut(s) 31
PdmI GAANNNNTTC 1 cut(s) 581
PfeI GAWTC 3 cut(s) 577, 582, 669
PinAI ACCGGT 1 cut(s) 169
PkrI GCNGC 6 cut(s) 117, 595, 628, 639, 649, 1000
PleI GAGTC 2 cut(s) 1034, 1081
PpsI GAGTC 2 cut(s) 1034, 1081
PsiI TTATAA 1 cut(s) 1005
Psp6I CCWGG 1 cut(s) 880
PspGI CCWGG 1 cut(s) 880
PspPI GGNCC 3 cut(s) 105, 702, 872
PstI CTGCAG 1 cut(s) 595
PvuII CAGCTG 1 cut(s) 647
SaqAI TTAA 1 cut(s) 894
SatI GCNGC 6 cut(s) 116, 594, 627, 638, 648, 999
Sau3AI GATC 5 cut(s) 69, 328, 550, 793, 1101
Sau96I GGNCC 3 cut(s) 105, 702, 872
SchI GAGTC 2 cut(s) 1035, 1082
ScrFI CCNGG 9 cut(s) 204, 277, 278, 562, 563, 838, 882, 901, 902
SduI GDGCHC 2 cut(s) 198, 377
SfaNI GCATC 2 cut(s) 430, 985
SfcI CTRYAG 3 cut(s) 243, 484, 591
SinI GGWCC 1 cut(s) 872
SmaI CCCGGG 3 cut(s) 278, 563, 902
SmlI CTYRAG 1 cut(s) 446
SmoI CTYRAG 1 cut(s) 446
SphI GCATGC 1 cut(s) 989
Sse9I AATT 6 cut(s) 19, 51, 307, 410, 876, 1040
SsiI CCGC 4 cut(s) 115, 543, 659, 685
SspMI CTAG 2 cut(s) 267, 534
StyD4I CCNGG 9 cut(s) 202, 275, 276, 560, 561, 836, 880, 899, 900
StyI CCWWGG 4 cut(s) 213, 266, 405, 597
TaaI ACNGT 2 cut(s) 485, 1022
TaiI ACGT 1 cut(s) 525
TaqI TCGA 2 cut(s) 377, 580
TaqII GACCGA 1 cut(s) 948
TasI AATT 6 cut(s) 19, 51, 307, 410, 876, 1040
TauI GCSGC 1 cut(s) 118
TfiI GAWTC 3 cut(s) 577, 582, 669
Tru1I TTAA 1 cut(s) 894
Tru9I TTAA 1 cut(s) 894
TscAI CASTG 2 cut(s) 72, 194
TseFI GTSAC 1 cut(s) 160
TseI GCWGC 5 cut(s) 593, 626, 637, 647, 998
Tsp45I GTSAC 1 cut(s) 160
TspDTI ATGAA 3 cut(s) 559, 590, 902
TspGWI ACGGA 2 cut(s) 31, 759
TspMI CCCGGG 3 cut(s) 276, 561, 900
TspRI CASTG 2 cut(s) 72, 194
VpaK11BI GGWCC 1 cut(s) 872
XapI RAATTY 1 cut(s) 19
XbaI TCTAGA 1 cut(s) 533
XceI RCATGY 1 cut(s) 989
XmaI CCCGGG 3 cut(s) 276, 561, 900
XmaJI CCTAGG 1 cut(s) 266
XmnI GAANNNNTTC 1 cut(s) 581
XspI CTAG 2 cut(s) 267, 534
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.