pycom05g27780

Protein of unknown function (DUF688)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0006667
Physical Location & Seq
Reverse (-)
28666115 .. 28667607
1493 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g27780.1

Sequence Viewer

Length: 1221 bp
ATGGAGGAAAGGAAACTGAACTTGAATGCACCTTTTCTGTCCGTGAGGCGGATTGCTACAACACCGGTGATCGCAGATAGAGAGAAAGAGAAGATAGTGCAGAGGCCCGATGGGCGGCACTGTCTTCCTCCTTATAAATCGGACTTTTGCTTGGAGCAGGTGACAGAACCGGTTGCAGTTCCTTTTCACTGGGAGCACATCCCCGGAAAAGCCAAAGTAGGCAAAGAGGCAGAGGCTACAGAGGAGGTTTCGGTTACTCCTAGGCTTCCACCCGGAAAGCTTATAAATGTTACAAAGCAAAGTTCGGAAAGGGATCATGGAGATCAAAATGGGGCAAGATCACCGCACTTTAATGCGTATTCTACGAAGGAAAATGGGAAGGAATTGAAGTGCTCAAAAGAGGTAATGAACCAGAGAGGAGCCTCGGAATCAGAGGACGAGGATGATATTTATTCGGATGCACTTGAGACAATTTCCCCTACAAAGTCTTTCTCCTTGAACTGTAGTGCAAGTTGTCGGAGTCAATCTCACGGTGCAGATGCAAAGTCATCTATAGCCTCCGCTGTTGATCCACAAACCCGGGACTTCTTGATGAATCGATTCTTACCTGCAGCCAAGGCAATGGCTTTGGAGACACCTCATGCTGCAAAGAAGCAACATACAGCTGCACCTGAACAGCCAAGACAAATCACGAGGGTAATCCGTGAGGATACAAGGCCCTTACCAAAGGAACAAAGGTCTCAAATGGAACTGCAACACAGACAATATGAAGAGGAAGAAGAAAGTGAAGGGGAAGATGACGAGTACGATCCCTCTGGCTCTGTTTCAGCAAAGGGTTGTGGGTTGTTTCCCCGCTTATGCTTGCGAAATTCTTTATGCCTCTTAGGTCCAATTCCAGGGATGAAAGTTAAGACCCGGGCTCTCACGTCTTCATCCTCTGGGGTTGCAAGACCTGAAAAATCTGCTTATGGTTGGTCTCTTAGCCAACCACTCAACAAGCATGCTTGGGATGCTGCTTATAAGCGCAAATCAAACTGTGGAGTCCAATCAGGTGAATTGCTGAAGTTGGACAATAAGAAGGCTGGTGAGTCCAATCGCTTTACACATTCTGCTAACGAAAGGCAGGTTATCTCCCTTGAGGCATTCACGAAGTGCTTGCATATCCCCCTACCGAAATGGAGCACCTTGTGGAGTCAAATCAGCAGAATTGCAGAAGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF688

Protein Analysis

407

Amino Acids

45.23

Weight (kDa)

7.98

Isoelectric Point (pI)

52.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF688 PF05097 1 - 371 5.4e-84 Protein of unknown function (DUF688)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 135, 284, 1020
AarI CACCTGC 1 cut(s) 148
Acc36I ACCTGC 3 cut(s) 148, 616, 1114
AciI CCGC 5 cut(s) 49, 115, 344, 561, 853
AclWI GGATC 3 cut(s) 321, 563, 803
AcsI RAATTY 1 cut(s) 868
AcuI CTGAAG 1 cut(s) 1082
AdeI CACNNNGTG 2 cut(s) 1152, 1188
AfaI GTAC 1 cut(s) 806
AfiI CCNNNNNNNGG 3 cut(s) 48, 114, 896
AgeI ACCGGT 2 cut(s) 64, 169
AgsI TTSAA 3 cut(s) 25, 388, 499
AjiI CACGTC 1 cut(s) 927
AjnI CCWGG 1 cut(s) 895
AluBI AGCT 2 cut(s) 280, 665
AluI AGCT 2 cut(s) 280, 665
Alw21I GWGCWC 3 cut(s) 198, 395, 1184
Alw26I GTCTC 4 cut(s) 461, 626, 744, 981
AlwI GGATC 3 cut(s) 321, 563, 803
Ama87I CYCGRG 2 cut(s) 579, 915
AoxI GGCC 2 cut(s) 104, 716
ApeKI GCWGC 4 cut(s) 611, 644, 665, 1013
ApoI RAATTY 1 cut(s) 868
AsiGI ACCGGT 2 cut(s) 64, 169
Asp700I GAANNNNTTC 1 cut(s) 599
AspA2I CCTAGG 1 cut(s) 260
AspLEI GCGC 1 cut(s) 1026
AspS9I GGNCC 3 cut(s) 105, 717, 887
AsuC2I CCSGG 6 cut(s) 204, 273, 580, 581, 916, 917
AsuHPI GGTGA 5 cut(s) 79, 172, 333, 1064, 1097
AvaI CYCGRG 2 cut(s) 579, 915
AvaII GGWCC 1 cut(s) 887
AvrII CCTAGG 1 cut(s) 260
BanII GRGCYC 1 cut(s) 922
BauI CACGAG 1 cut(s) 691
BbsI GAAGAC 2 cut(s) 116, 921
Bbv12I GWGCWC 3 cut(s) 198, 395, 1184
BbvI GCAGC 4 cut(s) 623, 631, 652, 1000
BccI CCATC 1 cut(s) 104
BcgI CGANNNNNNTGC 2 cut(s) 1138, 1172
BciT130I CCWGG 1 cut(s) 897
BciVI GTATCC 1 cut(s) 703
BcnI CCSGG 6 cut(s) 204, 273, 580, 581, 916, 917
BcoDI GTCTC 4 cut(s) 461, 626, 744, 981
BfaI CTAG 1 cut(s) 261
BfmI CTRYAG 4 cut(s) 237, 502, 552, 609
BfuAI ACCTGC 3 cut(s) 148, 616, 1114
BfuI GTATCC 1 cut(s) 703
BglI GCCNNNNNGGC 1 cut(s) 112
BisI GCNGC 5 cut(s) 116, 612, 645, 666, 1014
BlnI CCTAGG 1 cut(s) 260
BlsI GCNGC 5 cut(s) 117, 613, 646, 667, 1015
Bme1390I CCNGG 7 cut(s) 204, 273, 580, 581, 897, 916, 917
Bme18I GGWCC 1 cut(s) 887
BmeT110I CYCGRG 2 cut(s) 579, 915
BmgBI CACGTC 1 cut(s) 927
BmgT120I GGNCC 3 cut(s) 105, 717, 887
BmiI GGNNCC 1 cut(s) 421
BmrFI CCNGG 7 cut(s) 204, 273, 580, 581, 897, 916, 917
BmrI ACTGGG 1 cut(s) 199
BmsI GCATC 3 cut(s) 448, 529, 1000
BmuI ACTGGG 1 cut(s) 199
BpiI GAAGAC 2 cut(s) 116, 921
BplI GAGNNNNNCTC 2 cut(s) 511, 543
BpuEI CTTGAG 2 cut(s) 485, 1157
BpuMI CCSGG 6 cut(s) 204, 273, 580, 581, 916, 917
Bsa29I ATCGAT 1 cut(s) 598
BsaI GGTCTC 2 cut(s) 744, 981
BsaJI CCNNGG 7 cut(s) 202, 260, 423, 579, 615, 896, 915
BsaWI WCCGGW 2 cut(s) 64, 169
Bsc4I CCNNNNNNNGG 3 cut(s) 48, 114, 896
Bse118I RCCGGY 2 cut(s) 64, 169
Bse1I ACTGG 1 cut(s) 194
Bse3DI GCAATG 1 cut(s) 627
BseBI CCWGG 1 cut(s) 897
BseCI ATCGAT 1 cut(s) 598
BseDI CCNNGG 7 cut(s) 202, 260, 423, 579, 615, 896, 915
BseGI GGATG 6 cut(s) 198, 448, 463, 906, 932, 1015
BseLI CCNNNNNNNGG 3 cut(s) 48, 114, 896
BseMI GCAATG 1 cut(s) 627
BseNI ACTGG 1 cut(s) 194
BseRI GAGGAG 2 cut(s) 257, 432
BseXI GCAGC 4 cut(s) 623, 631, 652, 1000
BsgI GTGCAG 3 cut(s) 119, 555, 651
BshFI GGCC 2 cut(s) 106, 718
BshTI ACCGGT 2 cut(s) 64, 169
BshVI ATCGAT 1 cut(s) 598
BsiHKAI GWGCWC 3 cut(s) 198, 395, 1184
BsiHKCI CYCGRG 2 cut(s) 579, 915
BsiSI CCGG 6 cut(s) 65, 170, 204, 273, 580, 916
BslFI GGGAC 1 cut(s) 596
BslI CCNNNNNNNGG 3 cut(s) 48, 114, 896
BsmAI GTCTC 4 cut(s) 461, 626, 744, 981
BsmFI GGGAC 1 cut(s) 596
BsmI GAATGC 2 cut(s) 31, 1142
BsnI GGCC 2 cut(s) 106, 718
Bso31I GGTCTC 2 cut(s) 744, 981
BsoBI CYCGRG 2 cut(s) 579, 915
Bsp1286I GDGCHC 4 cut(s) 198, 395, 922, 1184
Bsp143I GATC 6 cut(s) 69, 313, 322, 338, 568, 808
BspACI CCGC 5 cut(s) 49, 115, 344, 561, 853
BspANI GGCC 2 cut(s) 106, 718
BspDI ATCGAT 1 cut(s) 598
BspLI GGNNCC 1 cut(s) 421
BspMAI CTGCAG 1 cut(s) 613
BspMI ACCTGC 3 cut(s) 148, 616, 1114
BspPI GGATC 3 cut(s) 321, 563, 803
BspTNI GGTCTC 2 cut(s) 744, 981
BsrDI GCAATG 1 cut(s) 627
BsrFI RCCGGY 2 cut(s) 64, 169
BsrI ACTGG 1 cut(s) 194
BssAI RCCGGY 2 cut(s) 64, 169
BssECI CCNNGG 7 cut(s) 202, 260, 423, 579, 615, 896, 915
BssMI GATC 6 cut(s) 69, 313, 322, 338, 568, 808
BssSI CACGAG 1 cut(s) 691
BssT1I CCWWGG 2 cut(s) 260, 615
Bst2BI CACGAG 1 cut(s) 691
Bst2UI CCWGG 1 cut(s) 897
Bst4CI ACNGT 4 cut(s) 122, 503, 533, 1037
Bst6I CTCTTC 1 cut(s) 765
BstC8I GCNNGC 3 cut(s) 863, 1002, 1157
BstDEI CTNAG 2 cut(s) 883, 980
BstF5I GGATG 6 cut(s) 198, 448, 463, 906, 932, 1015
BstHHI GCGC 1 cut(s) 1026
BstKTI GATC 6 cut(s) 72, 316, 325, 341, 571, 811
BstMAI GTCTC 4 cut(s) 461, 626, 744, 981
BstMBI GATC 6 cut(s) 69, 313, 322, 338, 568, 808
BstMWI GCNNNNNNNGC 3 cut(s) 112, 617, 1010
BstNI CCWGG 1 cut(s) 897
BstNSI RCATGY 1 cut(s) 1004
BstSCI CCNGG 7 cut(s) 202, 271, 578, 579, 895, 914, 915
BstSFI CTRYAG 4 cut(s) 237, 502, 552, 609
BstV1I GCAGC 4 cut(s) 623, 631, 652, 1000
BstV2I GAAGAC 2 cut(s) 116, 921
BstXI CCANNNNNNTGG 1 cut(s) 622
Bsu15I ATCGAT 1 cut(s) 598
BsuI GTATCC 1 cut(s) 703
BsuRI GGCC 2 cut(s) 106, 718
BsuTUI ATCGAT 1 cut(s) 598
BtrI CACGTC 1 cut(s) 927
BtsCI GGATG 6 cut(s) 198, 448, 463, 906, 932, 1015
BtsIMutI CAGTG 2 cut(s) 118, 187
BveI ACCTGC 3 cut(s) 148, 616, 1114
Cac8I GCNNGC 3 cut(s) 863, 1002, 1157
CfoI GCGC 1 cut(s) 1026
Cfr10I RCCGGY 2 cut(s) 64, 169
Cfr13I GGNCC 3 cut(s) 105, 717, 887
Cfr9I CCCGGG 2 cut(s) 579, 915
ClaI ATCGAT 1 cut(s) 598
Csp6I GTAC 1 cut(s) 805
CspAI ACCGGT 2 cut(s) 64, 169
CviAII CATG 3 cut(s) 317, 641, 1001
CviQI GTAC 1 cut(s) 805
DdeI CTNAG 2 cut(s) 883, 980
DpnI GATC 6 cut(s) 71, 315, 324, 340, 570, 810
DpnII GATC 6 cut(s) 69, 313, 322, 338, 568, 808
DraIII CACNNNGTG 2 cut(s) 1152, 1188
Eam1104I CTCTTC 1 cut(s) 765
EarI CTCTTC 1 cut(s) 765
EciI GGCGGA 1 cut(s) 64
Eco130I CCWWGG 2 cut(s) 260, 615
Eco24I GRGCYC 1 cut(s) 922
Eco31I GGTCTC 2 cut(s) 744, 981
Eco47I GGWCC 1 cut(s) 887
Eco57I CTGAAG 1 cut(s) 1082
Eco88I CYCGRG 2 cut(s) 579, 915
EcoO109I RGGNCCY 1 cut(s) 717
EcoRII CCWGG 1 cut(s) 895
EcoT14I CCWWGG 2 cut(s) 260, 615
EcoT38I GRGCYC 1 cut(s) 922
ErhI CCWWGG 2 cut(s) 260, 615
FaeI CATG 3 cut(s) 320, 644, 1004
FaqI GGGAC 1 cut(s) 596
FatI CATG 3 cut(s) 316, 640, 1000
FauI CCCGC 1 cut(s) 860
Fnu4HI GCNGC 5 cut(s) 116, 612, 645, 666, 1014
FokI GGATG 6 cut(s) 185, 455, 470, 913, 919, 1022
FriOI GRGCYC 1 cut(s) 922
Fsp4HI GCNGC 5 cut(s) 116, 612, 645, 666, 1014
FspBI CTAG 1 cut(s) 261
GlaI GCGC 1 cut(s) 1025
GluI GCNGC 5 cut(s) 116, 612, 645, 666, 1014
HaeIII GGCC 2 cut(s) 106, 718
HapII CCGG 6 cut(s) 65, 170, 204, 273, 580, 916
HhaI GCGC 1 cut(s) 1026
Hin1II CATG 3 cut(s) 320, 644, 1004
Hin6I GCGC 1 cut(s) 1024
HinP1I GCGC 1 cut(s) 1024
HindIII AAGCTT 1 cut(s) 278
HinfI GANTC 7 cut(s) 428, 520, 595, 600, 1041, 1088, 1192
HpaII CCGG 6 cut(s) 65, 170, 204, 273, 580, 916
HphI GGTGA 5 cut(s) 79, 172, 333, 1064, 1097
Hpy188I TCNGA 6 cut(s) 142, 307, 427, 433, 457, 519
Hpy188III TCNNGA 3 cut(s) 589, 691, 1147
HpyAV CCTTC 4 cut(s) 361, 373, 782, 1072
HpyCH4III ACNGT 4 cut(s) 122, 503, 533, 1037
HpyCH4IV ACGT 1 cut(s) 926
HpyF10VI GCNNNNNNNGC 3 cut(s) 112, 617, 1010
HpyF3I CTNAG 2 cut(s) 883, 980
HpySE526I ACGT 1 cut(s) 926
Hsp92II CATG 3 cut(s) 320, 644, 1004
HspAI GCGC 1 cut(s) 1024
Kzo9I GATC 6 cut(s) 69, 313, 322, 338, 568, 808
LmnI GCTCC 4 cut(s) 154, 193, 419, 1179
Lsp1109I GCAGC 4 cut(s) 623, 631, 652, 1000
LweI GCATC 3 cut(s) 448, 529, 1000
MaeI CTAG 1 cut(s) 261
MaeII ACGT 1 cut(s) 926
MaeIII GTNAC 3 cut(s) 160, 253, 289
MalI GATC 6 cut(s) 71, 315, 324, 340, 570, 810
MboI GATC 6 cut(s) 69, 313, 322, 338, 568, 808
MboII GAAGA 7 cut(s) 103, 116, 782, 788, 791, 806, 921
MhlI GDGCHC 4 cut(s) 198, 395, 922, 1184
MluCI AATT 6 cut(s) 383, 471, 868, 891, 1055, 1206
MlyI GAGTC 4 cut(s) 529, 1050, 1097, 1201
MmeI TCCRAC 2 cut(s) 497, 1047
MroXI GAANNNNTTC 1 cut(s) 599
MseI TTAA 2 cut(s) 351, 909
MslI CAYNNNNRTG 1 cut(s) 351
MspA1I CMGCKG 2 cut(s) 563, 665
MspI CCGG 6 cut(s) 65, 170, 204, 273, 580, 916
MspR9I CCNGG 7 cut(s) 204, 273, 580, 581, 897, 916, 917
Mva1269I GAATGC 2 cut(s) 31, 1142
MvaI CCWGG 1 cut(s) 897
MwoI GCNNNNNNNGC 3 cut(s) 112, 617, 1010
NciI CCSGG 6 cut(s) 204, 273, 580, 581, 916, 917
NdeII GATC 6 cut(s) 69, 313, 322, 338, 568, 808
NlaIII CATG 3 cut(s) 320, 644, 1004
NlaIV GGNNCC 1 cut(s) 421
NmuCI GTSAC 1 cut(s) 160
NspI RCATGY 1 cut(s) 1004
PaeI GCATGC 1 cut(s) 1004
PaqCI CACCTGC 1 cut(s) 148
PctI GAATGC 2 cut(s) 31, 1142
PdmI GAANNNNTTC 1 cut(s) 599
PfeI GAWTC 3 cut(s) 428, 595, 600
PinAI ACCGGT 2 cut(s) 64, 169
PkrI GCNGC 5 cut(s) 117, 613, 646, 667, 1015
PleI GAGTC 4 cut(s) 528, 1049, 1096, 1200
PpsI GAGTC 4 cut(s) 528, 1049, 1096, 1200
PsiI TTATAA 3 cut(s) 135, 284, 1020
Psp6I CCWGG 1 cut(s) 895
PspGI CCWGG 1 cut(s) 895
PspN4I GGNNCC 1 cut(s) 421
PspPI GGNCC 3 cut(s) 105, 717, 887
PstI CTGCAG 1 cut(s) 613
PvuII CAGCTG 1 cut(s) 665
RsaI GTAC 1 cut(s) 806
RsaNI GTAC 1 cut(s) 805
RseI CAYNNNNRTG 1 cut(s) 351
SaqAI TTAA 2 cut(s) 351, 909
SatI GCNGC 5 cut(s) 116, 612, 645, 666, 1014
Sau3AI GATC 6 cut(s) 69, 313, 322, 338, 568, 808
Sau96I GGNCC 3 cut(s) 105, 717, 887
SchI GAGTC 4 cut(s) 529, 1050, 1097, 1201
ScrFI CCNGG 7 cut(s) 204, 273, 580, 581, 897, 916, 917
SduI GDGCHC 4 cut(s) 198, 395, 922, 1184
SfaNI GCATC 3 cut(s) 448, 529, 1000
SfcI CTRYAG 4 cut(s) 237, 502, 552, 609
SgrAI CRCCGGYG 1 cut(s) 64
SinI GGWCC 1 cut(s) 887
SmaI CCCGGG 2 cut(s) 581, 917
SmiMI CAYNNNNRTG 1 cut(s) 351
SmlI CTYRAG 2 cut(s) 464, 1136
SmoI CTYRAG 2 cut(s) 464, 1136
SphI GCATGC 1 cut(s) 1004
Sse9I AATT 6 cut(s) 383, 471, 868, 891, 1055, 1206
SsiI CCGC 5 cut(s) 49, 115, 344, 561, 853
SspMI CTAG 1 cut(s) 261
StyD4I CCNGG 7 cut(s) 202, 271, 578, 579, 895, 914, 915
StyI CCWWGG 2 cut(s) 260, 615
TaaI ACNGT 4 cut(s) 122, 503, 533, 1037
TaiI ACGT 1 cut(s) 929
TaqI TCGA 1 cut(s) 598
TasI AATT 6 cut(s) 383, 471, 868, 891, 1055, 1206
TauI GCSGC 1 cut(s) 118
TfiI GAWTC 3 cut(s) 428, 595, 600
Tru1I TTAA 2 cut(s) 351, 909
Tru9I TTAA 2 cut(s) 351, 909
TscAI CASTG 2 cut(s) 125, 194
TseFI GTSAC 1 cut(s) 160
TseI GCWGC 4 cut(s) 611, 644, 665, 1013
Tsp45I GTSAC 1 cut(s) 160
TspDTI ATGAA 5 cut(s) 422, 608, 783, 917, 921
TspGWI ACGGA 2 cut(s) 31, 692
TspMI CCCGGG 2 cut(s) 579, 915
TspRI CASTG 2 cut(s) 125, 194
VpaK11BI GGWCC 1 cut(s) 887
XapI RAATTY 1 cut(s) 868
XceI RCATGY 1 cut(s) 1004
XmaI CCCGGG 2 cut(s) 579, 915
XmaJI CCTAGG 1 cut(s) 260
XmnI GAANNNNTTC 1 cut(s) 599
XspI CTAG 1 cut(s) 261
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.