Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr05

Orthogroup ID

OG0010001

Physical Location & Seq

Genomic Coordinates Forward (+)

43745048 .. 43747245

Gene Structure 2198 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD05G1304900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1266 bp

ATGGCGTCCGTACTCGGAAGTTGCTCGACAGCAGCAACTTTGGCCCCTCGACGTCTCTCATCTCGCACTTCCAGGACTTCAATTCCCTCTCTCTCTACATGCCCAGGGAAGAGTTTTGGGAGCAAGTTTTATGGAGGGATTGGGATTCATGGAAAGAAAAGGTCTCAATTCCATGTGACCAATGTTGCTACTGAAATCAGCCCTTCTGAGCAGGCGCAGCGGGTTGCTGCTAAGGAAAACCAGAGGCCAGTATATCCATTTGCTGCTATTGTAGGACAAGACGAGATGAAACTGTGTCTTCTGCTGAATGTGATTGATCCCAAGATTGGTGGTGTCATGATCATGGGAGATAGGGGAACTGGAAAATCCACAACCGTTAGGTCTTTGGTCGATTTGCTTCCTGAAATTAAGGTAGTTTCTGGTGACCCTTATAACTCAGACCCAGAAGATCCGGAGTCCATGGGTGCGGAAGTTAGAGAGAGCATTGTGAAGGGGGAGCAGCTTCCTGTTATCAAGACTAAGATCAACATGGTTGATTTACCCTTGGGTGCTACAGAAGATAGAGTCTGCGGGACAATTGACATTGAGAAAGCGCTCACTGAGGGAGTCAAGGCATTTGAGCCTGGCCTTCTTGCAAAAGCTAATAGAGGAATTCTGTATGTGGACGAAGTTAATCTTTTGGATGATCATTTAGTGGATGTTTTACTGGATTCTGCTGCATCGGGATGGAACACGGTGGAGAGAGAGGGTATTTCAATTTCACATCCTGCCCGGTTTATTTTGATTGGCTCGGGCAATCCAGAAGAAGGAGAGCTCAGGCCACAGTTGCTTGACCGTTTTGGAATGCACGCACAAGTTGGGACTGTGAGGGATGCGGAGCTCAGAGTGAAGATTGTTGAGGAGAGAGCTCGGTTTGACAAAAACCCTAAAGAATTTCGGGTTTCTTACGAAGCTGAGCAAGAAAAGCTTCAGGAGCAAATCGGCTCAGCTAGAAGTTATCTTCCATCTGTTCAGATTGATCAGGACCTCAAGGTGAAAATCTCCAAGGTTTGCGCAGAGTTGAATGTCGATGGATTGAGAGGAGACATAGTGACTAACAGAGCTGCAAAAGCCTTGGCTGCTCTAAAGGGAAGCGATAAGGTGTCTCCAGAGGATATTGCCACTGTCATCCCTAACTGCTTAAGACATCGTCTTCGGAAGGATCCTTTAGAGTCGATTGACTCCGGTTTACTTGTCATGGAGAAGTTCTATGAGGTGTTTAGCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005618 GO:0005622 GO:0005623 GO:0005737 GO:0006725 GO:0006778 GO:0006779 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009719 GO:0009725 GO:0009735 GO:0009987 GO:0010007 GO:0010033 GO:0015994 GO:0015995 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016851 GO:0016874 GO:0016887 GO:0017111 GO:0018130 GO:0019438 GO:0030312 GO:0032991 GO:0033013 GO:0033014 GO:0034641 GO:0042221 GO:0042440 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044249 GO:0044271 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046148 GO:0046483 GO:0050896 GO:0051002 GO:0051003 GO:0051186 GO:0051188 GO:0071704 GO:0071944 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901576 GO:1902494

KEGG Pathways

Metabolic & Signaling

ko00860 ko01100 ko01110 map00860 map01100 map01110

Pfam Domains

Protein Families

Mg_chelatase

Protein Analysis

422

Amino Acids

45.88

Weight (kDa)

5.86

Isoelectric Point (pI)

38.38

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Mg_chelatase	PF01078	197 - 267	3e-07	Magnesium chelatase, subunit ChlI
AAA_lid_2	PF17863	347 - 405	6.3e-19	AAA lid domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0010001)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G18480 AT5G45930
fragaria_vesca	FvH4_3g08130 FvH4_3g08130 FvH4_3g08130
malus_domestica	MD05G1304900.v1.1 MD10G1284000.v1.1
prunus_persica	Prupe.4G057500_v2.0.a1
pyrus_communis	pycom05g28220 pycom10g23690
rosa_chinensis	RchiOBHm_Chr5g0010061
rosa_laevigata	RLG00000031723
rosa_multiflora	Rmu_sc0002275.1_g000009 Rmu_sc0016197.1_g000002
rosa_roxburghii	Rroxscaffold_1G00066110
rosa_rugosa	Rorug04G0448900
rosa_samantha	Rh5AG079800 Rh5BG074600 Rh5CG087200 Rh5DG075300
rosa_wichuraiana	Rw5G007390

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	432
AatII	GACGTC	1 cut(s)	55
Acc16I	TGCGCA	1 cut(s)	1054
AccIII	TCCGGA	1 cut(s)	451
AciI	CCGC	4 cut(s)	220, 467, 570, 875
AclWI	GGATC	4 cut(s)	311, 443, 1196, 1209
AcsI	RAATTY	2 cut(s)	651, 932
AcuI	CTGAAG	1 cut(s)	953
AcyI	GRCGYC	2 cut(s)	5, 52
AfaI	GTAC	1 cut(s)	12
AfeI	AGCGCT	1 cut(s)	594
AfiI	CCNNNNNNNGG	2 cut(s)	326, 806
AflII	CTTAAG	1 cut(s)	1180
AgsI	TTSAA	3 cut(s)	81, 756, 1063
AjnI	CCWGG	3 cut(s)	71, 103, 622
Alw21I	GWGCWC	3 cut(s)	816, 882, 910
Alw26I	GTCTC	4 cut(s)	59, 168, 1077, 1149
AlwI	GGATC	4 cut(s)	311, 443, 1196, 1209
Ama87I	CYCGRG	1 cut(s)	790
Aor13HI	TCCGGA	1 cut(s)	451
Aor51HI	AGCGCT	1 cut(s)	594
AoxI	GGCC	4 cut(s)	42, 245, 625, 818
ApeKI	GCWGC	8 cut(s)	32, 217, 227, 263, 499, 716, 1103, 1118
ApoI	RAATTY	2 cut(s)	651, 932
ArsI	GACNNNNNNTTYG	2 cut(s)	1176, 1208
Asp700I	GAANNNNTTC	1 cut(s)	966
AspLEI	GCGC	3 cut(s)	217, 595, 1055
AspS9I	GGNCC	2 cut(s)	43, 1024
AsuC2I	CCSGG	1 cut(s)	772
AsuHPI	GGTGA	2 cut(s)	434, 1045
AvaI	CYCGRG	1 cut(s)	790
AvaII	GGWCC	1 cut(s)	1024
BamHI	GGATCC	1 cut(s)	1201
BanII	GRGCYC	3 cut(s)	816, 882, 910
BbsI	GAAGAC	2 cut(s)	290, 1184
Bbv12I	GWGCWC	3 cut(s)	816, 882, 910
BbvI	GCAGC	8 cut(s)	44, 214, 229, 250, 511, 703, 1090, 1105
BccI	CCATC	3 cut(s)	720, 1012, 1064
BciT130I	CCWGG	3 cut(s)	73, 105, 624
BclI	TGATCA	3 cut(s)	339, 685, 1018
BcnI	CCSGG	1 cut(s)	772
BcoDI	GTCTC	4 cut(s)	59, 168, 1077, 1149
BfaI	CTAG	1 cut(s)	990
BfmI	CTRYAG	1 cut(s)	552
BfoI	RGCGCY	1 cut(s)	596
BfrI	CTTAAG	1 cut(s)	1180
BisI	GCNGC	8 cut(s)	33, 218, 228, 264, 500, 717, 1104, 1119
BlpI	GCTNAGC	2 cut(s)	954, 985
BlsI	GCNGC	8 cut(s)	34, 219, 229, 265, 501, 718, 1105, 1120
Bme1390I	CCNGG	4 cut(s)	73, 105, 624, 772
Bme18I	GGWCC	1 cut(s)	1024
BmeT110I	CYCGRG	1 cut(s)	790
BmgT120I	GGNCC	2 cut(s)	43, 1024
BmiI	GGNNCC	2 cut(s)	45, 1203
BmrFI	CCNGG	4 cut(s)	73, 105, 624, 772
BmsI	GCATC	2 cut(s)	728, 862
BpiI	GAAGAC	2 cut(s)	290, 1184
BpmI	CTGGAG	1 cut(s)	1131
Bpu10I	CCTNAGC	2 cut(s)	231, 815
Bpu1102I	GCTNAGC	2 cut(s)	954, 985
BpuEI	CTTGAG	1 cut(s)	1013
BpuMI	CCSGG	1 cut(s)	772
BsaHI	GRCGYC	2 cut(s)	5, 52
BsaI	GGTCTC	1 cut(s)	168
BsaJI	CCNNGG	6 cut(s)	103, 104, 459, 543, 1044, 1113
BsaWI	WCCGGW	2 cut(s)	451, 1223
BsaXI	ACNNNNNCTCC	2 cut(s)	1074, 1104
Bsc4I	CCNNNNNNNGG	2 cut(s)	326, 806
Bse1I	ACTGG	3 cut(s)	248, 364, 711
BseAI	TCCGGA	1 cut(s)	451
BseBI	CCWGG	3 cut(s)	73, 105, 624
BseDI	CCNNGG	6 cut(s)	103, 104, 459, 543, 1044, 1113
BseGI	GGATG	6 cut(s)	688, 703, 731, 763, 877, 1167
BseLI	CCNNNNNNNGG	2 cut(s)	326, 806
BseMII	CTCAG	7 cut(s)	198, 450, 591, 829, 895, 945, 999
BseNI	ACTGG	3 cut(s)	248, 364, 711
BseRI	GAGGAG	2 cut(s)	914, 1095
BseXI	GCAGC	8 cut(s)	44, 214, 229, 250, 511, 703, 1090, 1105
BshFI	GGCC	4 cut(s)	44, 247, 627, 820
BsiHKAI	GWGCWC	3 cut(s)	816, 882, 910
BsiHKCI	CYCGRG	1 cut(s)	790
BsiSI	CCGG	3 cut(s)	452, 772, 1224
BslFI	GGGAC	2 cut(s)	586, 874
BslI	CCNNNNNNNGG	2 cut(s)	326, 806
BsmAI	GTCTC	4 cut(s)	59, 168, 1077, 1149
BsmBI	CGTCTC	1 cut(s)	59
BsmFI	GGGAC	2 cut(s)	586, 874
BsmI	GAATGC	1 cut(s)	849
BsnI	GGCC	4 cut(s)	44, 247, 627, 820
Bso31I	GGTCTC	1 cut(s)	168
BsoBI	CYCGRG	1 cut(s)	790
Bsp1286I	GDGCHC	3 cut(s)	816, 882, 910
Bsp13I	TCCGGA	1 cut(s)	451
Bsp143I	GATC	7 cut(s)	316, 339, 448, 522, 685, 1018, 1201
Bsp1720I	GCTNAGC	2 cut(s)	954, 985
Bsp19I	CCATGG	1 cut(s)	459
BspACI	CCGC	4 cut(s)	220, 467, 570, 875
BspANI	GGCC	4 cut(s)	44, 247, 627, 820
BspCNI	CTCAG	7 cut(s)	199, 449, 592, 828, 894, 946, 998
BspEI	TCCGGA	1 cut(s)	451
BspHI	TCATGA	1 cut(s)	336
BspLI	GGNNCC	2 cut(s)	45, 1203
BspPI	GGATC	4 cut(s)	311, 443, 1196, 1209
BspTI	CTTAAG	1 cut(s)	1180
BspTNI	GGTCTC	1 cut(s)	168
BsrI	ACTGG	3 cut(s)	248, 364, 711
BssECI	CCNNGG	6 cut(s)	103, 104, 459, 543, 1044, 1113
BssMI	GATC	7 cut(s)	316, 339, 448, 522, 685, 1018, 1201
BssNI	GRCGYC	2 cut(s)	5, 52
BssT1I	CCWWGG	4 cut(s)	459, 543, 1044, 1113
Bst2UI	CCWGG	3 cut(s)	73, 105, 624
Bst4CI	ACNGT	7 cut(s)	294, 376, 736, 825, 836, 865, 1165
Bst6I	CTCTTC	1 cut(s)	104
BstACI	GRCGYC	2 cut(s)	5, 52
BstAFI	CTTAAG	1 cut(s)	1180
BstC8I	GCNNGC	2 cut(s)	213, 849
BstDEI	CTNAG	9 cut(s)	207, 231, 436, 519, 600, 815, 881, 954, 985
BstDSI	CCRYGG	1 cut(s)	459
BstEII	GGTNACC	1 cut(s)	422
BstF5I	GGATG	6 cut(s)	688, 703, 731, 763, 877, 1167
BstH2I	RGCGCY	1 cut(s)	596
BstHHI	GCGC	3 cut(s)	217, 595, 1055
BstKTI	GATC	7 cut(s)	319, 342, 451, 525, 688, 1021, 1204
BstMAI	GTCTC	4 cut(s)	59, 168, 1077, 1149
BstMBI	GATC	7 cut(s)	316, 339, 448, 522, 685, 1018, 1201
BstMWI	GCNNNNNNNGC	7 cut(s)	41, 217, 826, 964, 973, 1109, 1118
BstNI	CCWGG	3 cut(s)	73, 105, 624
BstNSI	RCATGY	1 cut(s)	102
BstPI	GGTNACC	1 cut(s)	422
BstSCI	CCNGG	4 cut(s)	71, 103, 622, 770
BstSFI	CTRYAG	1 cut(s)	552
BstV1I	GCAGC	8 cut(s)	44, 214, 229, 250, 511, 703, 1090, 1105
BstV2I	GAAGAC	2 cut(s)	290, 1184
BstX2I	RGATCY	2 cut(s)	448, 1201
BstYI	RGATCY	2 cut(s)	448, 1201
BsuRI	GGCC	4 cut(s)	44, 247, 627, 820
BtgI	CCRYGG	1 cut(s)	459
BtsCI	GGATG	6 cut(s)	688, 703, 731, 763, 877, 1167
BtsIMutI	CAGTG	2 cut(s)	597, 1161
Cac8I	GCNNGC	2 cut(s)	213, 849
CciI	TCATGA	1 cut(s)	336
CfoI	GCGC	3 cut(s)	217, 595, 1055
Cfr13I	GGNCC	2 cut(s)	43, 1024
Csp6I	GTAC	1 cut(s)	11
CspCI	CAANNNNNGTGG	2 cut(s)	310, 345
CviAII	CATG	8 cut(s)	99, 149, 173, 337, 343, 460, 529, 1237
CviQI	GTAC	1 cut(s)	11
DdeI	CTNAG	9 cut(s)	207, 231, 436, 519, 600, 815, 881, 954, 985
DpnI	GATC	7 cut(s)	318, 341, 450, 524, 687, 1020, 1203
DpnII	GATC	7 cut(s)	316, 339, 448, 522, 685, 1018, 1201
Eam1104I	CTCTTC	1 cut(s)	104
EarI	CTCTTC	1 cut(s)	104
Ecl136II	GAGCTC	3 cut(s)	814, 880, 908
Eco130I	CCWWGG	4 cut(s)	459, 543, 1044, 1113
Eco24I	GRGCYC	3 cut(s)	816, 882, 910
Eco31I	GGTCTC	1 cut(s)	168
Eco47I	GGWCC	1 cut(s)	1024
Eco47III	AGCGCT	1 cut(s)	594
Eco53kI	GAGCTC	3 cut(s)	814, 880, 908
Eco57I	CTGAAG	1 cut(s)	953
Eco88I	CYCGRG	1 cut(s)	790
Eco91I	GGTNACC	1 cut(s)	422
EcoICRI	GAGCTC	3 cut(s)	814, 880, 908
EcoO109I	RGGNCCY	1 cut(s)	1024
EcoO65I	GGTNACC	1 cut(s)	422
EcoRI	GAATTC	1 cut(s)	651
EcoRII	CCWGG	3 cut(s)	71, 103, 622
EcoT14I	CCWWGG	4 cut(s)	459, 543, 1044, 1113
EcoT38I	GRGCYC	3 cut(s)	816, 882, 910
ErhI	CCWWGG	4 cut(s)	459, 543, 1044, 1113
Esp3I	CGTCTC	1 cut(s)	59
FaeI	CATG	8 cut(s)	102, 152, 176, 340, 346, 463, 532, 1240
FalI	AAGNNNNNCTT	4 cut(s)	660, 692, 951, 983
FaqI	GGGAC	2 cut(s)	586, 874
FatI	CATG	8 cut(s)	98, 148, 172, 336, 342, 459, 528, 1236
FauI	CCCGC	2 cut(s)	213, 563
FbaI	TGATCA	3 cut(s)	339, 685, 1018
Fnu4HI	GCNGC	8 cut(s)	33, 218, 228, 264, 500, 717, 1104, 1119
FokI	GGATG	6 cut(s)	695, 710, 738, 750, 884, 1154
FriOI	GRGCYC	3 cut(s)	816, 882, 910
Fsp4HI	GCNGC	8 cut(s)	33, 218, 228, 264, 500, 717, 1104, 1119
FspBI	CTAG	1 cut(s)	990
FspI	TGCGCA	1 cut(s)	1054
GlaI	GCGC	3 cut(s)	216, 594, 1054
GluI	GCNGC	8 cut(s)	33, 218, 228, 264, 500, 717, 1104, 1119
GsuI	CTGGAG	1 cut(s)	1131
HaeII	RGCGCY	1 cut(s)	596
HaeIII	GGCC	4 cut(s)	44, 247, 627, 820
HapII	CCGG	3 cut(s)	452, 772, 1224
HhaI	GCGC	3 cut(s)	217, 595, 1055
Hin1I	GRCGYC	2 cut(s)	5, 52
Hin1II	CATG	8 cut(s)	102, 152, 176, 340, 346, 463, 532, 1240
Hin6I	GCGC	3 cut(s)	215, 593, 1053
HinP1I	GCGC	3 cut(s)	215, 593, 1053
HindIII	AAGCTT	1 cut(s)	965
HinfI	GANTC	7 cut(s)	145, 455, 564, 606, 710, 1211, 1220
HpaII	CCGG	3 cut(s)	452, 772, 1224
HphI	GGTGA	2 cut(s)	434, 1045
Hpy166II	GTNNAC	2 cut(s)	664, 1229
Hpy188I	TCNGA	6 cut(s)	17, 208, 439, 884, 1014, 1197
Hpy188III	TCNNGA	9 cut(s)	337, 401, 452, 514, 723, 800, 971, 1022, 1148
Hpy8I	GTNNAC	2 cut(s)	664, 1229
Hpy99I	CGWCG	1 cut(s)	54
HpyAV	CCTTC	5 cut(s)	213, 484, 638, 800, 1192
HpyCH4III	ACNGT	7 cut(s)	294, 376, 736, 825, 836, 865, 1165
HpyCH4IV	ACGT	1 cut(s)	52
HpyCH4V	TGCA	4 cut(s)	635, 719, 847, 1106
HpyF10VI	GCNNNNNNNGC	7 cut(s)	41, 217, 826, 964, 973, 1109, 1118
HpyF3I	CTNAG	9 cut(s)	207, 231, 436, 519, 600, 815, 881, 954, 985
HpySE526I	ACGT	1 cut(s)	52
Hsp92I	GRCGYC	2 cut(s)	5, 52
Hsp92II	CATG	8 cut(s)	102, 152, 176, 340, 346, 463, 532, 1240
HspAI	GCGC	3 cut(s)	215, 593, 1053
Kpn2I	TCCGGA	1 cut(s)	451
Ksp22I	TGATCA	3 cut(s)	339, 685, 1018
Kzo9I	GATC	7 cut(s)	316, 339, 448, 522, 685, 1018, 1201
LmnI	GCTCC	4 cut(s)	120, 496, 877, 973
Lsp1109I	GCAGC	8 cut(s)	44, 214, 229, 250, 511, 703, 1090, 1105
LweI	GCATC	2 cut(s)	728, 862
MaeI	CTAG	1 cut(s)	990
MaeII	ACGT	1 cut(s)	52
MaeIII	GTNAC	3 cut(s)	175, 422, 1090
MalI	GATC	7 cut(s)	318, 341, 450, 524, 687, 1020, 1203
MboI	GATC	7 cut(s)	316, 339, 448, 522, 685, 1018, 1201
MboII	GAAGA	8 cut(s)	121, 290, 458, 569, 815, 901, 992, 1184
MfeI	CAATTG	1 cut(s)	576
MflI	RGATCY	2 cut(s)	448, 1201
MhlI	GDGCHC	3 cut(s)	816, 882, 910
MluCI	AATT	7 cut(s)	81, 167, 405, 576, 651, 756, 932
MlyI	GAGTC	5 cut(s)	464, 573, 615, 1214, 1220
MroI	TCCGGA	1 cut(s)	451
MroXI	GAANNNNTTC	1 cut(s)	966
MseI	TTAA	3 cut(s)	408, 672, 1181
MslI	CAYNNNNRTG	2 cut(s)	341, 724
MspA1I	CMGCKG	1 cut(s)	220
MspCI	CTTAAG	1 cut(s)	1180
MspI	CCGG	3 cut(s)	452, 772, 1224
MspR9I	CCNGG	4 cut(s)	73, 105, 624, 772
MunI	CAATTG	1 cut(s)	576
Mva1269I	GAATGC	1 cut(s)	849
MvaI	CCWGG	3 cut(s)	73, 105, 624
MwoI	GCNNNNNNNGC	7 cut(s)	41, 217, 826, 964, 973, 1109, 1118
NciI	CCSGG	1 cut(s)	772
NcoI	CCATGG	1 cut(s)	459
NdeII	GATC	7 cut(s)	316, 339, 448, 522, 685, 1018, 1201
NlaIII	CATG	8 cut(s)	102, 152, 176, 340, 346, 463, 532, 1240
NlaIV	GGNNCC	2 cut(s)	45, 1203
NmuCI	GTSAC	3 cut(s)	175, 422, 1090
NsbI	TGCGCA	1 cut(s)	1054
NspI	RCATGY	1 cut(s)	102
PagI	TCATGA	1 cut(s)	336
PasI	CCCWGGG	1 cut(s)	104
PctI	GAATGC	1 cut(s)	849
PdmI	GAANNNNTTC	1 cut(s)	966
PfeI	GAWTC	2 cut(s)	145, 710
PflFI	GACNNNGTC	1 cut(s)	1188
PfoI	TCCNGGA	1 cut(s)	71
PkrI	GCNGC	8 cut(s)	34, 219, 229, 265, 501, 718, 1105, 1120
PleI	GAGTC	5 cut(s)	463, 572, 614, 1214, 1219
PpsI	GAGTC	5 cut(s)	463, 572, 614, 1214, 1219
PpuMI	RGGWCCY	1 cut(s)	1024
PsiI	TTATAA	1 cut(s)	432
Psp124BI	GAGCTC	3 cut(s)	816, 882, 910
Psp5II	RGGWCCY	1 cut(s)	1024
Psp6I	CCWGG	3 cut(s)	71, 103, 622
PspEI	GGTNACC	1 cut(s)	422
PspGI	CCWGG	3 cut(s)	71, 103, 622
PspN4I	GGNNCC	2 cut(s)	45, 1203
PspPI	GGNCC	2 cut(s)	43, 1024
PspPPI	RGGWCCY	1 cut(s)	1024
PsuI	RGATCY	2 cut(s)	448, 1201
PsyI	GACNNNGTC	1 cut(s)	1188
RsaI	GTAC	1 cut(s)	12
RsaNI	GTAC	1 cut(s)	11
RseI	CAYNNNNRTG	2 cut(s)	341, 724
SacI	GAGCTC	3 cut(s)	816, 882, 910
SaqAI	TTAA	3 cut(s)	408, 672, 1181
SatI	GCNGC	8 cut(s)	33, 218, 228, 264, 500, 717, 1104, 1119
Sau3AI	GATC	7 cut(s)	316, 339, 448, 522, 685, 1018, 1201
Sau96I	GGNCC	2 cut(s)	43, 1024
SchI	GAGTC	5 cut(s)	464, 573, 615, 1214, 1220
ScrFI	CCNGG	4 cut(s)	73, 105, 624, 772
SduI	GDGCHC	3 cut(s)	816, 882, 910
SfaNI	GCATC	2 cut(s)	728, 862
SfcI	CTRYAG	1 cut(s)	552
SinI	GGWCC	1 cut(s)	1024
SmiMI	CAYNNNNRTG	2 cut(s)	341, 724
SmlI	CTYRAG	2 cut(s)	1028, 1180
SmoI	CTYRAG	2 cut(s)	1028, 1180
Sse9I	AATT	7 cut(s)	81, 167, 405, 576, 651, 756, 932
SsiI	CCGC	4 cut(s)	220, 467, 570, 875
SspMI	CTAG	1 cut(s)	990
SstI	GAGCTC	3 cut(s)	816, 882, 910
StyD4I	CCNGG	4 cut(s)	71, 103, 622, 770
StyI	CCWWGG	4 cut(s)	459, 543, 1044, 1113
TaaI	ACNGT	7 cut(s)	294, 376, 736, 825, 836, 865, 1165
TaiI	ACGT	1 cut(s)	55
TaqI	TCGA	5 cut(s)	26, 49, 390, 1068, 1214
TasI	AATT	7 cut(s)	81, 167, 405, 576, 651, 756, 932
TfiI	GAWTC	2 cut(s)	145, 710
Tru1I	TTAA	3 cut(s)	408, 672, 1181
Tru9I	TTAA	3 cut(s)	408, 672, 1181
TscAI	CASTG	2 cut(s)	604, 1168
TseFI	GTSAC	3 cut(s)	175, 422, 1090
TseI	GCWGC	8 cut(s)	32, 217, 227, 263, 499, 716, 1103, 1118
Tsp45I	GTSAC	3 cut(s)	175, 422, 1090
TspDTI	ATGAA	2 cut(s)	137, 302
TspRI	CASTG	2 cut(s)	604, 1168
Tth111I	GACNNNGTC	1 cut(s)	1188
Vha464I	CTTAAG	1 cut(s)	1180
VpaK11BI	GGWCC	1 cut(s)	1024
XapI	RAATTY	2 cut(s)	651, 932
XceI	RCATGY	1 cut(s)	102
XmnI	GAANNNNTTC	1 cut(s)	966
XspI	CTAG	1 cut(s)	990
ZraI	GACGTC	1 cut(s)	53

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.