Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr05

Orthogroup ID

OG0006350

Physical Location & Seq

Genomic Coordinates Forward (+)

44006027 .. 44010980

Gene Structure 4954 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD05G1309400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2142 bp

ATGGCGGGTCTAATTGATCTGAACAGTGCGACGGAGGACGAGGAAACGCCATCGTCCGGCTCGCCGTCTTCGGCTTCCTCTGTTTCCGACGCTCTGGGTTCGTCGGCGTCGGTGTGCATGGAGCTCTGGCACGCCTGCGCGGGCCCACTGATTTCGCTGCCGAAGAAAGGGAGTGTGGTGGTGTATCTGCCGCAGGGCCACCTGGAGCAAGTCTCGGATTTTCCGACCTCGGCTTATGATCTCCCGCCCCACCTCTTCTGTCGGGTTGTCGATGTCAAGCTCCATGCTGAGACTGGCACTGACGATGTCTTCGCTCAGGTTTCCCTTGTTCCTGAAAGTGAGGAAATTGAGCACAGATTGCGGGAAGGGGTAACCGATGCAGATGCCGAGGAGGACGTTGAGGCAATGGGGACGTCAACCACACCCCACATGTTCTGCAAAACCCTTACTGCTTCTGATACTAGCACTCACGGAGGCTTCTCTGTGCCTCGTCGTGCTGCCGAGGATTGCTTTCCTCCCCTGGATTACACTCAACAAAGGCCTTCACAAGAGCTTGTAGCAAAGGATCTGCATGGCCTGGAGTGGAGGTTCCGGCATATCTATAGGGGGCAGCCGCGGAGGCATTTGCTCACCACTGGGTGGAGTGCGTTTGTGAACAAGAAGAAGCTCGTCTCTGGAGATGCAGTGCTGTTTCTTAGGGGTGACGATGGAGAACTGAGGCTAGGAATTAGAAGGGCAGCCCAGTTTAAAAGTTCTGCTACTTGTCCAACTCTTTGTAGCCAGCAATTGAACTATAGCACTATCACTGATGTGGTGAATGCTATATTCGCGAATAATGCTTTTAATGTGTACTACAATCCAAGGTCCAGCTCTTCTGAATTCATAATACCTTCCCATAAGTTTTTGAGGAGCCTTGATCATTGTTTTTGTGCTGGAATGAGGATCAAAATGCGTTTTGAAACTGAAGATGCAGCAGAGCGAAGATACACTGGGTTGATAACGGGGATTAGTGAATTGGATCCTGTAAGATGGCCTGGTTCAAAATGGAGATGCCTAGTTGTCAGGTGGGATGATATAGACACAAGCAAGCATGGCAGGGTTTCCCCATGGGAAGTTGAGCGATCTGGTTCTGTTTCTAGTTCCCATACCCTAATGACAACTGGCTTGAAGCGGTCCAGGATTGGCTTGTCTGCAACAAAACCAGAATGTCCAGTTCCTAATGGGATTGGAACATCAGACTTTGGGGAATCTTTAAGGTTCCAGAAGGTCTTGCAAGGTCAAGAAATTTCGGGGTTTGATACTCCTTTCAGTGGTTTAGGTGGTCTGAATTCGCATCCATCTGAAGCAAGGAGAGTCTTCCACGGTTCCGGTGGTTCTGGGATTGCTGCTGGGGGTAATGGTCTCAGACAGTCACTTGTGGATTCTGAGATTGCCTCAAAAGGCATAGGCTTTGGTGAATCATTCCGATTCCATAAGGTCTTGCAAGGTCAAGAAATATTTCCAAGCTCACCATATGGAAGAGCTCCCGCTTCTAATGAAGCTCATGAATATGGTGGACCTGGACTCTATGATGGTTTTCAGGTGCCTGGCTTTAGGAATGGATGCTCCACCATGATGCAGAGCAATAATACAAATGTGCACTCATCTGCCCCATCTGTGCAAGTTTCATCACCTTCGTCTGTGTTAATGTTCCAGCAAGCAATGAATCCAGTTGCGGAATTCAACTCGGTATACAATGGCCATAACCAAGAGGACCATAGAGTAAATCGGACTCCACATGTCTTGGAACATGATGGTGGAAGGCAAACATCATCCTCATTCGGTGAACGTAACTTCAGCAGGGAAGATCGTGGAGGCACACATTCTTACAATCAGCATGGTATTTCACCTCATCCAGTTATAAGTCAATCAACAATTAGTGGCAGCCAGGATTCTGTTTCACCAATCAAAGGTAGCTGTAGACTCTTTGGTTTCTCATTGTCCGAGGACAAATGTGTCCCGGATCAAGAGGGCAACCCCAATGTTGGAGTGCAGTTTCATTCAAAGCCTCCTTTGATGACCTCAACAGTTGGAATAACCTGTACTAAAGATTGGGCTTTTGACTGGCGCGGAGAAAGGATGGAAAGCTGTTTACAAGGATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0001708 GO:0003006 GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0007275 GO:0008150 GO:0008152 GO:0009719 GO:0009725 GO:0009733 GO:0009791 GO:0009850 GO:0009888 GO:0009889 GO:0009908 GO:0009987 GO:0010022 GO:0010033 GO:0010050 GO:0010073 GO:0010158 GO:0010468 GO:0010556 GO:0010582 GO:0010817 GO:0019219 GO:0019222 GO:0022414 GO:0030154 GO:0031323 GO:0031326 GO:0032501 GO:0032502 GO:0042221 GO:0042445 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044424 GO:0044464 GO:0045165 GO:0048367 GO:0048507 GO:0048608 GO:0048731 GO:0048856 GO:0048869 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051252 GO:0060255 GO:0061458 GO:0065007 GO:0065008 GO:0080090 GO:0090567 GO:0097159 GO:0140110 GO:1901363 GO:1903506 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

ko04075 map04075

Pfam Domains

Protein Families

Auxin_resp B3

Protein Analysis

714

Amino Acids

77.77

Weight (kDa)

6.03

Isoelectric Point (pI)

52.16

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
B3	PF02362	145 - 246	2.8e-23	B3 DNA binding domain
BRWD_AD	PF25313	252 - 374	7.3e-07	BRWD/PHIP ancillary domain-like domain
ARF_AD	PF06507	294 - 374	3.7e-32	Auxin response factor ancillary domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006350)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G33860 AT2G33860
fragaria_vesca	FvH4_3g08450 FvH4_3g08450 FvH4_3g08450 FvH4_3g08450 FvH4_3g08450 FvH4_3g08450
malus_domestica	MD05G1309400.v1.1 MD10G1287900.v1.1
prunus_persica	Prupe.4G053800_v2.0.a1 Prupe.4G053800_v2.0.a1 Prupe.4G053800_v2.0.a1
pyrus_communis	pycom05g28570 pycom10g24090
rosa_chinensis	RchiOBHm_Chr5g0009381
rosa_laevigata	RLG00000031657
rosa_multiflora	Rmu_sc0002711.1_g000019
rosa_roxburghii	Rroxscaffold_1G00066710 Rroxscaffold_1G00067410
rosa_rugosa	Rorug04G0442500
rosa_samantha	Rh5AG073100 Rh5AG073700 Rh5BG069500 Rh5CG081900 Rh5DG069800
rosa_wichuraiana	Rw5G006830

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1901
AasI	GACNNNNNNGTC	1 cut(s)	1994
AatII	GACGTC	1 cut(s)	416
AccB1I	GGYRCC	1 cut(s)	1582
AccB7I	CCANNNNNTGG	1 cut(s)	639
AccI	GTMKAC	2 cut(s)	1731, 1960
AccII	CGCG	4 cut(s)	140, 616, 830, 2109
AclWI	GGATC	5 cut(s)	573, 950, 1013, 1026, 2010
AcoI	YGGCCR	1 cut(s)	1738
AcsI	RAATTY	4 cut(s)	878, 1284, 1327, 1718
AcuI	CTGAAG	3 cut(s)	984, 1362, 1819
AcyI	GRCGYC	2 cut(s)	107, 413
AdeI	CACNNNGTG	1 cut(s)	639
AfaI	GTAC	2 cut(s)	851, 2083
AfiI	CCNNNNNNNGG	6 cut(s)	56, 167, 639, 1310, 1949, 2024
AflIII	ACRYGT	2 cut(s)	429, 1777
AgsI	TTSAA	6 cut(s)	790, 959, 1041, 1168, 1723, 2043
AjnI	CCWGG	8 cut(s)	201, 519, 576, 1033, 1175, 1558, 1585, 1926
AleI	CACNNNNGTG	1 cut(s)	809
AloI	GAACNNNNNNTCC	2 cut(s)	572, 604
Alw21I	GWGCWC	4 cut(s)	126, 354, 1525, 1641
Alw26I	GTCTC	4 cut(s)	217, 284, 676, 1406
Alw44I	GTGCAC	1 cut(s)	1637
AlwI	GGATC	5 cut(s)	573, 950, 1013, 1026, 2010
AoxI	GGCC	6 cut(s)	142, 196, 539, 574, 1031, 1738
ApaI	GGGCCC	1 cut(s)	146
ApaLI	GTGCAC	1 cut(s)	1637
ApeKI	GCWGC	7 cut(s)	157, 497, 610, 737, 971, 1385, 1923
ApoI	RAATTY	4 cut(s)	878, 1284, 1327, 1718
ArsI	GACNNNNNNTTYG	2 cut(s)	293, 325
Asp700I	GAANNNNTTC	1 cut(s)	1497
AspLEI	GCGC	2 cut(s)	140, 2109
AspS9I	GGNCC	7 cut(s)	142, 143, 196, 864, 1173, 1556, 1753
AsuC2I	CCSGG	1 cut(s)	2000
AsuHPI	GGTGA	9 cut(s)	622, 713, 826, 1466, 1500, 1662, 1835, 1878, 1932
AvaII	GGWCC	4 cut(s)	864, 1173, 1556, 1753
BaeGI	GKGCMC	2 cut(s)	146, 1641
BalI	TGGCCA	1 cut(s)	1740
BamHI	GGATCC	1 cut(s)	1018
BanI	GGYRCC	1 cut(s)	1582
BanII	GRGCYC	3 cut(s)	126, 146, 1525
BbsI	GAAGAC	3 cut(s)	60, 301, 1348
Bbv12I	GWGCWC	4 cut(s)	126, 354, 1525, 1641
BbvI	GCAGC	7 cut(s)	144, 484, 622, 749, 983, 1372, 1935
BccI	CCATC	8 cut(s)	58, 701, 1023, 1345, 1565, 1660, 1787, 2113
BceAI	ACGGC	1 cut(s)	49
BcgI	CGANNNNNNTGC	2 cut(s)	365, 399
BciT130I	CCWGG	8 cut(s)	203, 521, 578, 1035, 1177, 1560, 1587, 1928
BclI	TGATCA	1 cut(s)	916
BcnI	CCSGG	1 cut(s)	2000
BcoDI	GTCTC	4 cut(s)	217, 284, 676, 1406
BfaI	CTAG	4 cut(s)	462, 722, 1055, 1137
BfmI	CTRYAG	3 cut(s)	601, 793, 1957
BglI	GCCNNNNNGGC	1 cut(s)	619
BisI	GCNGC	9 cut(s)	158, 191, 498, 611, 614, 738, 972, 1386, 1924
BlsI	GCNGC	9 cut(s)	159, 192, 499, 612, 615, 739, 973, 1387, 1925
Bme1390I	CCNGG	9 cut(s)	203, 521, 578, 1035, 1177, 1560, 1587, 1928, 2000
Bme18I	GGWCC	4 cut(s)	864, 1173, 1556, 1753
BmgT120I	GGNCC	7 cut(s)	142, 143, 196, 864, 1173, 1556, 1753
BmiI	GGNNCC	7 cut(s)	144, 590, 911, 1020, 1259, 1366, 1584
BmrFI	CCNGG	9 cut(s)	203, 521, 578, 1035, 1177, 1560, 1587, 1928, 2000
BmrI	ACTGGG	3 cut(s)	645, 736, 999
BmsI	GCATC	8 cut(s)	367, 373, 670, 958, 1040, 1342, 1592, 1605
BmuI	ACTGGG	3 cut(s)	645, 736, 999
BpiI	GAAGAC	3 cut(s)	60, 301, 1348
BplI	GAGNNNNNCTC	4 cut(s)	197, 229, 1418, 1450
BpmI	CTGGAG	3 cut(s)	224, 599, 696
Bpu10I	CCTNAGC	1 cut(s)	315
BpuMI	CCSGG	1 cut(s)	2000
BsaHI	GRCGYC	2 cut(s)	107, 413
BsaI	GGTCTC	1 cut(s)	1406
BsaJI	CCNNGG	9 cut(s)	228, 387, 501, 519, 614, 860, 1106, 1360, 1983
BsaWI	WCCGGW	1 cut(s)	1367
BsaXI	ACNNNNNCTCC	4 cut(s)	572, 602, 669, 699
Bsc4I	CCNNNNNNNGG	6 cut(s)	56, 167, 639, 1310, 1949, 2024
Bse1I	ACTGG	9 cut(s)	298, 640, 742, 994, 1165, 1211, 1709, 1895, 2108
Bse3DI	GCAATG	2 cut(s)	411, 1707
BseBI	CCWGG	8 cut(s)	203, 521, 578, 1035, 1177, 1560, 1587, 1928
BseDI	CCNNGG	9 cut(s)	228, 387, 501, 519, 614, 860, 1106, 1360, 1983
BseGI	GGATG	6 cut(s)	1075, 1333, 1607, 1811, 1891, 2124
BseLI	CCNNNNNNNGG	6 cut(s)	56, 167, 639, 1310, 1949, 2024
BseMI	GCAATG	2 cut(s)	411, 1707
BseMII	CTCAG	5 cut(s)	279, 329, 707, 1416, 1417
BseNI	ACTGG	9 cut(s)	298, 640, 742, 994, 1165, 1211, 1709, 1895, 2108
BseRI	GAGGAG	2 cut(s)	404, 922
BseSI	GKGCMC	2 cut(s)	146, 1641
BseXI	GCAGC	7 cut(s)	144, 484, 622, 749, 983, 1372, 1935
BseYI	CCCAGC	1 cut(s)	1388
BsgI	GTGCAG	1 cut(s)	2051
Bsh1236I	CGCG	4 cut(s)	140, 616, 830, 2109
BshFI	GGCC	6 cut(s)	144, 198, 541, 576, 1033, 1740
BshNI	GGYRCC	1 cut(s)	1582
BsiHKAI	GWGCWC	4 cut(s)	126, 354, 1525, 1641
BsiSI	CCGG	4 cut(s)	57, 592, 1368, 2000
BslFI	GGGAC	2 cut(s)	424, 1982
BslI	CCNNNNNNNGG	6 cut(s)	56, 167, 639, 1310, 1949, 2024
BsmAI	GTCTC	4 cut(s)	217, 284, 676, 1406
BsmBI	CGTCTC	1 cut(s)	676
BsmFI	GGGAC	2 cut(s)	424, 1982
BsmI	GAATGC	1 cut(s)	823
BsnI	GGCC	6 cut(s)	144, 198, 541, 576, 1033, 1740
Bso31I	GGTCTC	1 cut(s)	1406
Bsp120I	GGGCCC	1 cut(s)	142
Bsp1286I	GDGCHC	5 cut(s)	126, 146, 354, 1525, 1641
Bsp143I	GATC	9 cut(s)	16, 238, 565, 916, 942, 1018, 1121, 1846, 2002
Bsp19I	CCATGG	1 cut(s)	1106
Bsp68I	TCGCGA	1 cut(s)	830
BspANI	GGCC	6 cut(s)	144, 198, 541, 576, 1033, 1740
BspCNI	CTCAG	5 cut(s)	280, 328, 708, 1416, 1417
BspFNI	CGCG	4 cut(s)	140, 616, 830, 2109
BspHI	TCATGA	1 cut(s)	1543
BspLI	GGNNCC	7 cut(s)	144, 590, 911, 1020, 1259, 1366, 1584
BspPI	GGATC	5 cut(s)	573, 950, 1013, 1026, 2010
BspQI	GCTCTTC	2 cut(s)	877, 1513
BspT107I	GGYRCC	1 cut(s)	1582
BspTNI	GGTCTC	1 cut(s)	1406
BsrDI	GCAATG	2 cut(s)	411, 1707
BsrI	ACTGG	9 cut(s)	298, 640, 742, 994, 1165, 1211, 1709, 1895, 2108
BssECI	CCNNGG	9 cut(s)	228, 387, 501, 519, 614, 860, 1106, 1360, 1983
BssMI	GATC	9 cut(s)	16, 238, 565, 916, 942, 1018, 1121, 1846, 2002
BssNAI	GTATAC	1 cut(s)	1732
BssNI	GRCGYC	2 cut(s)	107, 413
BssT1I	CCWWGG	2 cut(s)	860, 1106
Bst1107I	GTATAC	1 cut(s)	1732
Bst2UI	CCWGG	8 cut(s)	203, 521, 578, 1035, 1177, 1560, 1587, 1928
Bst4CI	ACNGT	4 cut(s)	26, 1364, 1410, 2068
Bst6I	CTCTTC	3 cut(s)	260, 877, 1513
BstACI	GRCGYC	2 cut(s)	107, 413
BstAPI	GCANNNNNTGC	1 cut(s)	358
BstC8I	GCNNGC	7 cut(s)	62, 132, 136, 142, 782, 1088, 1698
BstDEI	CTNAG	6 cut(s)	288, 315, 695, 716, 1403, 1425
BstDSI	CCRYGG	3 cut(s)	614, 1106, 1360
BstEII	GGTNACC	1 cut(s)	370
BstF5I	GGATG	6 cut(s)	1075, 1333, 1607, 1811, 1891, 2124
BstFNI	CGCG	4 cut(s)	140, 616, 830, 2109
BstHHI	GCGC	2 cut(s)	140, 2109
BstKTI	GATC	9 cut(s)	19, 241, 568, 919, 945, 1021, 1124, 1849, 2005
BstMAI	GTCTC	4 cut(s)	217, 284, 676, 1406
BstMBI	GATC	9 cut(s)	16, 238, 565, 916, 942, 1018, 1121, 1846, 2002
BstMWI	GCNNNNNNNGC	5 cut(s)	358, 619, 827, 836, 1092
BstNI	CCWGG	8 cut(s)	203, 521, 578, 1035, 1177, 1560, 1587, 1928
BstNSI	RCATGY	2 cut(s)	433, 1781
BstPI	GGTNACC	1 cut(s)	370
BstSCI	CCNGG	9 cut(s)	201, 519, 576, 1033, 1175, 1558, 1585, 1926, 1998
BstSFI	CTRYAG	3 cut(s)	601, 793, 1957
BstSLI	GKGCMC	2 cut(s)	146, 1641
BstUI	CGCG	4 cut(s)	140, 616, 830, 2109
BstV1I	GCAGC	7 cut(s)	144, 484, 622, 749, 983, 1372, 1935
BstV2I	GAAGAC	3 cut(s)	60, 301, 1348
BstX2I	RGATCY	2 cut(s)	565, 1018
BstYI	RGATCY	2 cut(s)	565, 1018
BstZ17I	GTATAC	1 cut(s)	1732
BsuRI	GGCC	6 cut(s)	144, 198, 541, 576, 1033, 1740
BtgI	CCRYGG	3 cut(s)	614, 1106, 1360
BtsCI	GGATG	6 cut(s)	1075, 1333, 1607, 1811, 1891, 2124
BtsI	GCAGTG	1 cut(s)	690
BtsIMutI	CAGTG	8 cut(s)	31, 146, 297, 633, 690, 804, 987, 1315
BtuMI	TCGCGA	1 cut(s)	830
Cac8I	GCNNGC	7 cut(s)	62, 132, 136, 142, 782, 1088, 1698
CciI	TCATGA	1 cut(s)	1543
CfoI	GCGC	2 cut(s)	140, 2109
Cfr13I	GGNCC	7 cut(s)	142, 143, 196, 864, 1173, 1556, 1753
Cfr42I	CCGCGG	1 cut(s)	617
CseI	GACGC	2 cut(s)	96, 98
Csp6I	GTAC	2 cut(s)	850, 2082
CspCI	CAANNNNNGTGG	2 cut(s)	1764, 1799
CviQI	GTAC	2 cut(s)	850, 2082
DdeI	CTNAG	6 cut(s)	288, 315, 695, 716, 1403, 1425
DpnI	GATC	9 cut(s)	18, 240, 567, 918, 944, 1020, 1123, 1848, 2004
DpnII	GATC	9 cut(s)	16, 238, 565, 916, 942, 1018, 1121, 1846, 2002
DraI	TTTAAA	1 cut(s)	748
DraIII	CACNNNGTG	1 cut(s)	639
DrdI	GACNNNNNNGTC	1 cut(s)	1994
DseDI	GACNNNNNNGTC	1 cut(s)	1994
EaeI	YGGCCR	1 cut(s)	1738
Eam1104I	CTCTTC	3 cut(s)	260, 877, 1513
EarI	CTCTTC	3 cut(s)	260, 877, 1513
Ecl136II	GAGCTC	2 cut(s)	124, 1523
Eco130I	CCWWGG	2 cut(s)	860, 1106
Eco147I	AGGCCT	1 cut(s)	541
Eco24I	GRGCYC	3 cut(s)	126, 146, 1525
Eco31I	GGTCTC	1 cut(s)	1406
Eco47I	GGWCC	4 cut(s)	864, 1173, 1556, 1753
Eco53kI	GAGCTC	2 cut(s)	124, 1523
Eco57I	CTGAAG	3 cut(s)	984, 1362, 1819
Eco91I	GGTNACC	1 cut(s)	370
EcoICRI	GAGCTC	2 cut(s)	124, 1523
EcoO65I	GGTNACC	1 cut(s)	370
EcoRI	GAATTC	3 cut(s)	878, 1327, 1718
EcoRII	CCWGG	8 cut(s)	201, 519, 576, 1033, 1175, 1558, 1585, 1926
EcoT14I	CCWWGG	2 cut(s)	860, 1106
EcoT38I	GRGCYC	3 cut(s)	126, 146, 1525
ErhI	CCWWGG	2 cut(s)	860, 1106
Esp3I	CGTCTC	1 cut(s)	676
FaqI	GGGAC	2 cut(s)	424, 1982
FauI	CCCGC	4 cut(s)	133, 252, 354, 1534
FauNDI	CATATG	1 cut(s)	1513
FbaI	TGATCA	1 cut(s)	916
FblI	GTMKAC	2 cut(s)	1731, 1960
Fnu4HI	GCNGC	9 cut(s)	158, 191, 498, 611, 614, 738, 972, 1386, 1924
FokI	GGATG	6 cut(s)	1082, 1320, 1614, 1798, 1878, 2131
FriOI	GRGCYC	3 cut(s)	126, 146, 1525
Fsp4HI	GCNGC	9 cut(s)	158, 191, 498, 611, 614, 738, 972, 1386, 1924
FspBI	CTAG	4 cut(s)	462, 722, 1055, 1137
GlaI	GCGC	2 cut(s)	139, 2108
GluI	GCNGC	9 cut(s)	158, 191, 498, 611, 614, 738, 972, 1386, 1924
GsaI	CCCAGC	1 cut(s)	1392
GsuI	CTGGAG	3 cut(s)	224, 599, 696
HaeIII	GGCC	6 cut(s)	144, 198, 541, 576, 1033, 1740
HapII	CCGG	4 cut(s)	57, 592, 1368, 2000
HgaI	GACGC	2 cut(s)	96, 98
HhaI	GCGC	2 cut(s)	140, 2109
Hin1I	GRCGYC	2 cut(s)	107, 413
Hin6I	GCGC	2 cut(s)	138, 2107
HinP1I	GCGC	2 cut(s)	138, 2107
HincII	GTYRAC	1 cut(s)	417
HindII	GTYRAC	1 cut(s)	417
HpaII	CCGG	4 cut(s)	57, 592, 1368, 2000
HphI	GGTGA	9 cut(s)	622, 713, 826, 1466, 1500, 1662, 1835, 1878, 1932
Hpy166II	GTNNAC	9 cut(s)	417, 655, 850, 1556, 1639, 1732, 1826, 1961, 2132
Hpy188III	TCNNGA	8 cut(s)	332, 675, 829, 1261, 1280, 1490, 1544, 2006
Hpy8I	GTNNAC	9 cut(s)	417, 655, 850, 1556, 1639, 1732, 1826, 1961, 2132
Hpy99I	CGWCG	5 cut(s)	34, 92, 106, 112, 495
HpyAV	CCTTC	7 cut(s)	359, 552, 726, 900, 1258, 1683, 1794
HpyCH4III	ACNGT	4 cut(s)	26, 1364, 1410, 2068
HpyCH4IV	ACGT	3 cut(s)	396, 413, 1828
HpyF10VI	GCNNNNNNNGC	5 cut(s)	358, 619, 827, 836, 1092
HpyF3I	CTNAG	6 cut(s)	288, 315, 695, 716, 1403, 1425
HpySE526I	ACGT	3 cut(s)	396, 413, 1828
Hsp92I	GRCGYC	2 cut(s)	107, 413
HspAI	GCGC	2 cut(s)	138, 2107
Ksp22I	TGATCA	1 cut(s)	916
KspI	CCGCGG	1 cut(s)	617
Kzo9I	GATC	9 cut(s)	16, 238, 565, 916, 942, 1018, 1121, 1846, 2002
LguI	GCTCTTC	2 cut(s)	877, 1513
LmnI	GCTCC	6 cut(s)	121, 205, 285, 909, 1528, 1610
Lsp1109I	GCAGC	7 cut(s)	144, 484, 622, 749, 983, 1372, 1935
LweI	GCATC	8 cut(s)	367, 373, 670, 958, 1040, 1342, 1592, 1605
MaeI	CTAG	4 cut(s)	462, 722, 1055, 1137
MaeII	ACGT	3 cut(s)	396, 413, 1828
MaeIII	GTNAC	4 cut(s)	370, 701, 1410, 1829
MalI	GATC	9 cut(s)	18, 240, 567, 918, 944, 1020, 1123, 1848, 2004
MboI	GATC	9 cut(s)	16, 238, 565, 916, 942, 1018, 1121, 1846, 2002
MfeI	CAATTG	1 cut(s)	785
MflI	RGATCY	2 cut(s)	565, 1018
MhlI	GDGCHC	5 cut(s)	126, 146, 354, 1525, 1641
MlsI	TGGCCA	1 cut(s)	1740
MluNI	TGGCCA	1 cut(s)	1740
MlyI	GAGTC	4 cut(s)	1362, 1557, 1765, 1956
MmeI	TCCRAC	5 cut(s)	111, 248, 791, 2005, 2050
Mox20I	TGGCCA	1 cut(s)	1740
MroXI	GAANNNNTTC	1 cut(s)	1497
MscI	TGGCCA	1 cut(s)	1740
MseI	TTAA	4 cut(s)	747, 843, 1253, 1685
MslI	CAYNNNNRTG	4 cut(s)	809, 1548, 1613, 1794
Msp20I	TGGCCA	1 cut(s)	1740
MspA1I	CMGCKG	1 cut(s)	616
MspI	CCGG	4 cut(s)	57, 592, 1368, 2000
MspR9I	CCNGG	9 cut(s)	203, 521, 578, 1035, 1177, 1560, 1587, 1928, 2000
MunI	CAATTG	1 cut(s)	785
Mva1269I	GAATGC	1 cut(s)	823
MvaI	CCWGG	8 cut(s)	203, 521, 578, 1035, 1177, 1560, 1587, 1928
MvnI	CGCG	4 cut(s)	140, 616, 830, 2109
MwoI	GCNNNNNNNGC	5 cut(s)	358, 619, 827, 836, 1092
NciI	CCSGG	1 cut(s)	2000
NcoI	CCATGG	1 cut(s)	1106
NdeI	CATATG	1 cut(s)	1513
NdeII	GATC	9 cut(s)	16, 238, 565, 916, 942, 1018, 1121, 1846, 2002
NlaIV	GGNNCC	7 cut(s)	144, 590, 911, 1020, 1259, 1366, 1584
NmeAIII	GCCGAG	3 cut(s)	209, 412, 526
NmuCI	GTSAC	2 cut(s)	701, 1410
NruI	TCGCGA	1 cut(s)	830
NspI	RCATGY	2 cut(s)	433, 1781
OliI	CACNNNNGTG	1 cut(s)	809
PagI	TCATGA	1 cut(s)	1543
PceI	AGGCCT	1 cut(s)	541
PciI	ACATGT	2 cut(s)	429, 1777
PciSI	GCTCTTC	2 cut(s)	877, 1513
PcsI	WCGNNNNNNNCGW	1 cut(s)	221
PctI	GAATGC	1 cut(s)	823
PdmI	GAANNNNTTC	1 cut(s)	1497
PfeI	GAWTC	6 cut(s)	1247, 1421, 1457, 1467, 1705, 1931
PflFI	GACNNNGTC	1 cut(s)	305
PflMI	CCANNNNNTGG	1 cut(s)	639
PfoI	TCCNGGA	2 cut(s)	1175, 1998
PkrI	GCNGC	9 cut(s)	159, 192, 499, 612, 615, 739, 973, 1387, 1925
PleI	GAGTC	4 cut(s)	1361, 1557, 1765, 1956
PpsI	GAGTC	4 cut(s)	1361, 1557, 1765, 1956
PscI	ACATGT	2 cut(s)	429, 1777
PsiI	TTATAA	1 cut(s)	1901
Psp124BI	GAGCTC	2 cut(s)	126, 1525
Psp6I	CCWGG	8 cut(s)	201, 519, 576, 1033, 1175, 1558, 1585, 1926
PspEI	GGTNACC	1 cut(s)	370
PspFI	CCCAGC	1 cut(s)	1388
PspGI	CCWGG	8 cut(s)	201, 519, 576, 1033, 1175, 1558, 1585, 1926
PspN4I	GGNNCC	7 cut(s)	144, 590, 911, 1020, 1259, 1366, 1584
PspOMI	GGGCCC	1 cut(s)	142
PspPI	GGNCC	7 cut(s)	142, 143, 196, 864, 1173, 1556, 1753
PsuI	RGATCY	2 cut(s)	565, 1018
PsyI	GACNNNGTC	1 cut(s)	305
RruI	TCGCGA	1 cut(s)	830
RsaI	GTAC	2 cut(s)	851, 2083
RsaNI	GTAC	2 cut(s)	850, 2082
RseI	CAYNNNNRTG	4 cut(s)	809, 1548, 1613, 1794
SacI	GAGCTC	2 cut(s)	126, 1525
SacII	CCGCGG	1 cut(s)	617
SapI	GCTCTTC	2 cut(s)	877, 1513
SaqAI	TTAA	4 cut(s)	747, 843, 1253, 1685
SatI	GCNGC	9 cut(s)	158, 191, 498, 611, 614, 738, 972, 1386, 1924
Sau3AI	GATC	9 cut(s)	16, 238, 565, 916, 942, 1018, 1121, 1846, 2002
Sau96I	GGNCC	7 cut(s)	142, 143, 196, 864, 1173, 1556, 1753
SchI	GAGTC	4 cut(s)	1362, 1557, 1765, 1956
ScrFI	CCNGG	9 cut(s)	203, 521, 578, 1035, 1177, 1560, 1587, 1928, 2000
SduI	GDGCHC	5 cut(s)	126, 146, 354, 1525, 1641
SfaNI	GCATC	8 cut(s)	367, 373, 670, 958, 1040, 1342, 1592, 1605
SfcI	CTRYAG	3 cut(s)	601, 793, 1957
Sfr303I	CCGCGG	1 cut(s)	617
SgrBI	CCGCGG	1 cut(s)	617
SinI	GGWCC	4 cut(s)	864, 1173, 1556, 1753
SmiMI	CAYNNNNRTG	4 cut(s)	809, 1548, 1613, 1794
SseBI	AGGCCT	1 cut(s)	541
SspI	AATATT	1 cut(s)	1497
SspMI	CTAG	4 cut(s)	462, 722, 1055, 1137
SstI	GAGCTC	2 cut(s)	126, 1525
StuI	AGGCCT	1 cut(s)	541
StyD4I	CCNGG	9 cut(s)	201, 519, 576, 1033, 1175, 1558, 1585, 1926, 1998
StyI	CCWWGG	2 cut(s)	860, 1106
TaaI	ACNGT	4 cut(s)	26, 1364, 1410, 2068
TaiI	ACGT	3 cut(s)	399, 416, 1831
TaqI	TCGA	1 cut(s)	270
TatI	WGTACW	2 cut(s)	849, 2081
TauI	GCSGC	2 cut(s)	193, 616
TfiI	GAWTC	6 cut(s)	1247, 1421, 1457, 1467, 1705, 1931
Tru1I	TTAA	4 cut(s)	747, 843, 1253, 1685
Tru9I	TTAA	4 cut(s)	747, 843, 1253, 1685
TscAI	CASTG	8 cut(s)	31, 153, 304, 640, 690, 811, 994, 1315
TseFI	GTSAC	2 cut(s)	701, 1410
TseI	GCWGC	7 cut(s)	157, 497, 610, 737, 971, 1385, 1923
Tsp45I	GTSAC	2 cut(s)	701, 1410
TspDTI	ATGAA	6 cut(s)	871, 1551, 1560, 1656, 1718, 2027
TspGWI	ACGGA	2 cut(s)	47, 486
TspRI	CASTG	8 cut(s)	31, 153, 304, 640, 690, 811, 994, 1315
Tth111I	GACNNNGTC	1 cut(s)	305
Van91I	CCANNNNNTGG	1 cut(s)	639
VneI	GTGCAC	1 cut(s)	1637
VpaK11BI	GGWCC	4 cut(s)	864, 1173, 1556, 1753
XapI	RAATTY	4 cut(s)	878, 1284, 1327, 1718
XceI	RCATGY	2 cut(s)	433, 1781
XcmI	CCANNNNNNNNNTGG	2 cut(s)	290, 1367
XmiI	GTMKAC	2 cut(s)	1731, 1960
XmnI	GAANNNNTTC	1 cut(s)	1497
XspI	CTAG	4 cut(s)	462, 722, 1055, 1137
ZraI	GACGTC	1 cut(s)	414

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.