Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr10

Orthogroup ID

OG0006350

Physical Location & Seq

Genomic Coordinates Forward (+)

37705581 .. 37710841

Gene Structure 5261 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD10G1287900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2139 bp

ATGGCTGGTCTAATCGATCTGAACAGTGCGACGGAGGACGAGGAAACGCCGTCGTCTGTCTCGTCGTCTTCGGCCTCCTCTGTCTCCGACGCTTTGGGTTCATCGGCGTCGGTGTGCTTGGAGCTCTGGCACGCCTGCGCGGGCCCGTTGATTTCGCTGCCGAAGAAAGGGACTGTGGTGGTGTATCTGCCGCAGGGCCACCTGGAGCAAGTATCGGATTTTCCGACCTCGGCTTATGATCTCCCGCCCCACCTCTTCTGTCGGGTTGTTGATGTCAAGCTTCATGCTGAGTCTGGCACTGACGATGTCTTCGCTCAGGTTTCACTTGTTCCTGAAAGTGAGGAAATTGAGCACAGATTGCTGGAAGGGGAAACCGATGCAGATGGCGAGGAGGATGTTGAGGCAATGGGGAAGTCAACCACACCCCACATGTTCTGCAAAACCCTTACTGCTTCCGATACTAGCACTCACGGAGGGTTCTCTGTGCCTCGTCGTGCTGCCGAGGATTGCTTTCCTCCCTTGGATTACACTCAACAAAGGCCTTCACAAGAGCTTGTAGCAAAGGATCTGCATGGCCTGGAGTGGAGGTTCCGCCATATCTATAGGGGGCAGCCACGGAGGCATTTGCTTACCACTGGGTGGAGTGCTTTTGTGAACAAGAAGAAGCTCGTCTCTGGGGATGCAGTGCTGTTTCTTAGGGGTGACGATGGAGAACTGAGGCTAGGAATTAGAAGGGCAGCCCAGGTTAAAAGTTCTGCTACTTGTCCAACTCTTTGTAGCCAGCAATTGAACTATAGCACTGTCACCGATGTGGTGAATGCTGTATCCACAAAGAATGCTTTTAATGTGTACTACAATCCAAGGGCCAGCTCTTCTGAATTCATAATACCTTCCCGTAAGTTTTTGAGGAGCCTTGATCATTGCTTTTCTGCTGGAATGAGGTTCAAAATGCGTTTTGAAACTGAAGATGCAGCAGAGCGAAGATACACTGGGTTGATAACAGGGATTGGTGCATTGGATCCTATAAGATGGCCTGGTTCAAAATGGAAATGCCTAGTTGTCAGGTGGGATGATATAGACACAAGTAAGCATGGCAGGGTTTCCCCATGGGAAATTGAGCCATCTGGTTCTGTTTCTAGTTCCCATAGCCTAATGGGAACTGGTTTGAAGCGGTCCAGGATTGGTTTGTCTGCAACAAAACCAGAATTTCCAGTTCCTAATGGGAATGGAGCATCAGACTTTGGGGAATCTTTAAGGTTCCAGAAGGTCTTGCAAGGTCAAGAAATTTCGGGGTTTGATACTCCTTTCAGCGGTCTAGGTGTTCAGAATCCGCATCCATCTGAAGCAAGGAGAGTCTTCCAGGGTTCCGGTGGTTCTGGGATTGCTGCTGGAAGTAACGGTCTCCGACAGTCACTTGTGGATTCTGAGATTGCCTCAAAAGGCATAGGCTTTGGTGAATCACTCCGATTCCATAAGGTCTTGCAAGGTCAAGAAATATTTCCAAGCTCACCATATGGAAGAGCTCCCGCTTCTAACAAAGCTCATGAATATGGTGGACCTGGAGTCTATGATGGTTTTCAGGTGCCCGGCTTTAGAAATGGATGGTCCACCATGATGCAGAGCAATAATACACAAGTGCACCCATCTGCCACATCTGTGCAAGTTTCATCACCATCGTCTGTGTTAATGTTCCAGCAAGCAATCAACCCAGTTACGGAATTCAATTCGGTATACAATGGTCATAACCAAGAGGAACATAGAGTTATACATCCAACCCCATATGTCTCTGAATATGACGGTGGAAGGAAAACATCATCCTCATTTGGTGAACGTAACTTCAGCAGGGAAGATCATGGAGGCACGCATTCTTACAATCAGCATGGTATTTCAAATGATCCAGTTATAAGTCGATCAACATTTAGTGGCAGTCAGGATTCAATTTCACCAACCAAAGGTAGCTGTAGACTCTTTGGTTTCTCATTGTCCGAGGACAAATGTGTCCCGGATCAAGCCCCTACTGCTGGAGTGCGGTTTCATTCAAAGCCTCCTTTGATGACTTCAGCAGTTGGAATTACCTGTACTAAAGATTGGGCTTTTGACTGGCGCGGAGAAAGGATGGAAAGCTGTTTACAAGGATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0001708 GO:0003006 GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0007275 GO:0008150 GO:0008152 GO:0009719 GO:0009725 GO:0009733 GO:0009791 GO:0009850 GO:0009888 GO:0009889 GO:0009908 GO:0009987 GO:0010022 GO:0010033 GO:0010050 GO:0010073 GO:0010158 GO:0010468 GO:0010556 GO:0010582 GO:0010817 GO:0019219 GO:0019222 GO:0022414 GO:0030154 GO:0031323 GO:0031326 GO:0032501 GO:0032502 GO:0042221 GO:0042445 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044424 GO:0044464 GO:0045165 GO:0048367 GO:0048507 GO:0048608 GO:0048731 GO:0048856 GO:0048869 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051252 GO:0060255 GO:0061458 GO:0065007 GO:0065008 GO:0080090 GO:0090567 GO:0097159 GO:0140110 GO:1901363 GO:1903506 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

ko04075 map04075

Pfam Domains

Protein Families

Auxin_resp B3

Protein Analysis

713

Amino Acids

77.57

Weight (kDa)

6.19

Isoelectric Point (pI)

50.9

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
B3	PF02362	145 - 246	2.8e-23	B3 DNA binding domain
BRWD_AD	PF25313	249 - 375	2.3e-08	BRWD/PHIP ancillary domain-like domain
ARF_AD	PF06507	294 - 375	4.9e-32	Auxin response factor ancillary domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006350)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G33860 AT2G33860
fragaria_vesca	FvH4_3g08450 FvH4_3g08450 FvH4_3g08450 FvH4_3g08450 FvH4_3g08450 FvH4_3g08450
malus_domestica	MD05G1309400.v1.1 MD10G1287900.v1.1
prunus_persica	Prupe.4G053800_v2.0.a1 Prupe.4G053800_v2.0.a1 Prupe.4G053800_v2.0.a1
pyrus_communis	pycom05g28570 pycom10g24090
rosa_chinensis	RchiOBHm_Chr5g0009381
rosa_laevigata	RLG00000031657
rosa_multiflora	Rmu_sc0002711.1_g000019
rosa_roxburghii	Rroxscaffold_1G00066710 Rroxscaffold_1G00067410
rosa_rugosa	Rorug04G0442500
rosa_samantha	Rh5AG073100 Rh5AG073700 Rh5BG069500 Rh5CG081900 Rh5DG069800
rosa_wichuraiana	Rw5G006830

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1904
AasI	GACNNNNNNGTC	1 cut(s)	1997
AccB1I	GGYRCC	1 cut(s)	1582
AccB7I	CCANNNNNTGG	1 cut(s)	639
AccI	GTMKAC	2 cut(s)	1731, 1963
AccII	CGCG	2 cut(s)	140, 2106
AclWI	GGATC	5 cut(s)	573, 1013, 1026, 1889, 2013
AcsI	RAATTY	4 cut(s)	878, 1205, 1284, 1718
AcuI	CTGAAG	4 cut(s)	984, 1362, 1822, 2043
AcyI	GRCGYC	1 cut(s)	107
AdeI	CACNNNGTG	1 cut(s)	639
AfaI	GTAC	2 cut(s)	851, 2080
AfiI	CCNNNNNNNGG	6 cut(s)	167, 639, 1310, 1714, 1952, 2021
AflIII	ACRYGT	1 cut(s)	429
AgsI	TTSAA	9 cut(s)	790, 946, 959, 1041, 1168, 1723, 1890, 1938, 2040
AhdI	GACNNNNNGTC	1 cut(s)	1562
AjnI	CCWGG	7 cut(s)	201, 576, 741, 1033, 1175, 1359, 1558
AleI	CACNNNNGTG	2 cut(s)	809, 1655
AloI	GAACNNNNNNTCC	2 cut(s)	572, 604
Alw21I	GWGCWC	4 cut(s)	126, 354, 1525, 1641
Alw26I	GTCTC	5 cut(s)	64, 88, 676, 1406, 1789
Alw44I	GTGCAC	1 cut(s)	1637
AlwI	GGATC	5 cut(s)	573, 1013, 1026, 1889, 2013
AoxI	GGCC	7 cut(s)	72, 142, 196, 539, 574, 864, 1031
ApaI	GGGCCC	1 cut(s)	146
ApaLI	GTGCAC	1 cut(s)	1637
ApeKI	GCWGC	6 cut(s)	157, 497, 610, 737, 971, 1385
ApoI	RAATTY	4 cut(s)	878, 1205, 1284, 1718
ArsI	GACNNNNNNTTYG	2 cut(s)	293, 325
Asp700I	GAANNNNTTC	1 cut(s)	1497
AspLEI	GCGC	2 cut(s)	140, 2106
AspS9I	GGNCC	7 cut(s)	142, 143, 196, 864, 1173, 1556, 1605
AsuC2I	CCSGG	2 cut(s)	1587, 2003
AsuHPI	GGTGA	8 cut(s)	713, 796, 826, 1466, 1500, 1662, 1838, 1935
AvaII	GGWCC	3 cut(s)	1173, 1556, 1605
BaeGI	GKGCMC	3 cut(s)	146, 1587, 1641
BamHI	GGATCC	1 cut(s)	1018
BanI	GGYRCC	1 cut(s)	1582
BanII	GRGCYC	3 cut(s)	126, 146, 1525
BbsI	GAAGAC	3 cut(s)	60, 301, 1348
Bbv12I	GWGCWC	4 cut(s)	126, 354, 1525, 1641
BbvI	GCAGC	6 cut(s)	144, 484, 622, 749, 983, 1372
BceAI	ACGGC	1 cut(s)	34
BciT130I	CCWGG	7 cut(s)	203, 578, 743, 1035, 1177, 1361, 1560
BciVI	GTATCC	1 cut(s)	835
BclI	TGATCA	1 cut(s)	916
BcnI	CCSGG	2 cut(s)	1587, 2003
BcoDI	GTCTC	5 cut(s)	64, 88, 676, 1406, 1789
BfaI	CTAG	5 cut(s)	462, 722, 1055, 1137, 1316
BfmI	CTRYAG	3 cut(s)	601, 793, 1960
BfuI	GTATCC	1 cut(s)	835
BglI	GCCNNNNNGGC	1 cut(s)	619
BisI	GCNGC	7 cut(s)	158, 191, 498, 611, 738, 972, 1386
BlsI	GCNGC	7 cut(s)	159, 192, 499, 612, 739, 973, 1387
Bme1390I	CCNGG	9 cut(s)	203, 578, 743, 1035, 1177, 1361, 1560, 1587, 2003
Bme18I	GGWCC	3 cut(s)	1173, 1556, 1605
BmeRI	GACNNNNNGTC	1 cut(s)	1562
BmgT120I	GGNCC	7 cut(s)	142, 143, 196, 864, 1173, 1556, 1605
BmiI	GGNNCC	7 cut(s)	144, 590, 911, 1020, 1259, 1366, 1584
BmrFI	CCNGG	9 cut(s)	203, 578, 743, 1035, 1177, 1361, 1560, 1587, 2003
BmrI	ACTGGG	3 cut(s)	645, 999, 1703
BmsI	GCATC	6 cut(s)	367, 670, 958, 1241, 1342, 1605
BmuI	ACTGGG	3 cut(s)	645, 999, 1703
BpiI	GAAGAC	3 cut(s)	60, 301, 1348
BplI	GAGNNNNNCTC	2 cut(s)	1418, 1450
BpmI	CTGGAG	4 cut(s)	224, 599, 1581, 2043
Bpu10I	CCTNAGC	1 cut(s)	315
BpuMI	CCSGG	2 cut(s)	1587, 2003
Bsa29I	ATCGAT	1 cut(s)	15
BsaHI	GRCGYC	1 cut(s)	107
BsaI	GGTCTC	1 cut(s)	1406
BsaJI	CCNNGG	9 cut(s)	228, 501, 519, 614, 741, 860, 1106, 1360, 1986
BsaWI	WCCGGW	1 cut(s)	1367
BsaXI	ACNNNNNCTCC	2 cut(s)	572, 602
Bsc4I	CCNNNNNNNGG	6 cut(s)	167, 639, 1310, 1714, 1952, 2021
Bse1I	ACTGG	7 cut(s)	640, 994, 1165, 1211, 1709, 1898, 2105
Bse3DI	GCAATG	2 cut(s)	411, 919
BseBI	CCWGG	7 cut(s)	203, 578, 743, 1035, 1177, 1361, 1560
BseCI	ATCGAT	1 cut(s)	15
BseDI	CCNNGG	9 cut(s)	228, 501, 519, 614, 741, 860, 1106, 1360, 1986
BseGI	GGATG	8 cut(s)	400, 685, 1075, 1333, 1607, 1768, 1814, 2121
BseLI	CCNNNNNNNGG	6 cut(s)	167, 639, 1310, 1714, 1952, 2021
BseMI	GCAATG	2 cut(s)	411, 919
BseMII	CTCAG	4 cut(s)	279, 329, 707, 1416
BseNI	ACTGG	7 cut(s)	640, 994, 1165, 1211, 1709, 1898, 2105
BseRI	GAGGAG	3 cut(s)	67, 404, 922
BseSI	GKGCMC	3 cut(s)	146, 1587, 1641
BseXI	GCAGC	6 cut(s)	144, 484, 622, 749, 983, 1372
Bsh1236I	CGCG	2 cut(s)	140, 2106
BshFI	GGCC	7 cut(s)	74, 144, 198, 541, 576, 866, 1033
BshNI	GGYRCC	1 cut(s)	1582
BshVI	ATCGAT	1 cut(s)	15
BsiHKAI	GWGCWC	4 cut(s)	126, 354, 1525, 1641
BsiSI	CCGG	3 cut(s)	1368, 1587, 2003
BslFI	GGGAC	2 cut(s)	184, 1985
BslI	CCNNNNNNNGG	6 cut(s)	167, 639, 1310, 1714, 1952, 2021
BsmAI	GTCTC	5 cut(s)	64, 88, 676, 1406, 1789
BsmBI	CGTCTC	1 cut(s)	676
BsmFI	GGGAC	2 cut(s)	184, 1985
BsmI	GAATGC	3 cut(s)	823, 841, 1864
BsnI	GGCC	7 cut(s)	74, 144, 198, 541, 576, 866, 1033
Bso31I	GGTCTC	1 cut(s)	1406
Bsp120I	GGGCCC	1 cut(s)	142
Bsp1286I	GDGCHC	6 cut(s)	126, 146, 354, 1525, 1587, 1641
Bsp143I	GATC	9 cut(s)	16, 238, 565, 916, 1018, 1849, 1894, 1910, 2005
Bsp19I	CCATGG	1 cut(s)	1106
BspANI	GGCC	7 cut(s)	74, 144, 198, 541, 576, 866, 1033
BspCNI	CTCAG	4 cut(s)	280, 328, 708, 1417
BspDI	ATCGAT	1 cut(s)	15
BspFNI	CGCG	2 cut(s)	140, 2106
BspHI	TCATGA	1 cut(s)	1543
BspLI	GGNNCC	7 cut(s)	144, 590, 911, 1020, 1259, 1366, 1584
BspPI	GGATC	5 cut(s)	573, 1013, 1026, 1889, 2013
BspQI	GCTCTTC	2 cut(s)	877, 1513
BspT107I	GGYRCC	1 cut(s)	1582
BspTNI	GGTCTC	1 cut(s)	1406
BsrDI	GCAATG	2 cut(s)	411, 919
BsrI	ACTGG	7 cut(s)	640, 994, 1165, 1211, 1709, 1898, 2105
BssECI	CCNNGG	9 cut(s)	228, 501, 519, 614, 741, 860, 1106, 1360, 1986
BssMI	GATC	9 cut(s)	16, 238, 565, 916, 1018, 1849, 1894, 1910, 2005
BssNAI	GTATAC	1 cut(s)	1732
BssNI	GRCGYC	1 cut(s)	107
BssT1I	CCWWGG	3 cut(s)	519, 860, 1106
Bst1107I	GTATAC	1 cut(s)	1732
Bst2UI	CCWGG	7 cut(s)	203, 578, 743, 1035, 1177, 1361, 1560
Bst4CI	ACNGT	6 cut(s)	26, 175, 802, 1400, 1410, 1799
Bst6I	CTCTTC	3 cut(s)	260, 877, 1513
BstACI	GRCGYC	1 cut(s)	107
BstAPI	GCANNNNNTGC	1 cut(s)	358
BstC8I	GCNNGC	7 cut(s)	132, 136, 142, 782, 868, 1698, 1862
BstDEI	CTNAG	5 cut(s)	288, 315, 695, 716, 1425
BstDSI	CCRYGG	2 cut(s)	614, 1106
BstF5I	GGATG	8 cut(s)	400, 685, 1075, 1333, 1607, 1768, 1814, 2121
BstFNI	CGCG	2 cut(s)	140, 2106
BstHHI	GCGC	2 cut(s)	140, 2106
BstKTI	GATC	9 cut(s)	19, 241, 568, 919, 1021, 1852, 1897, 1913, 2008
BstMAI	GTCTC	5 cut(s)	64, 88, 676, 1406, 1789
BstMBI	GATC	9 cut(s)	16, 238, 565, 916, 1018, 1849, 1894, 1910, 2005
BstMWI	GCNNNNNNNGC	3 cut(s)	358, 619, 2018
BstNI	CCWGG	7 cut(s)	203, 578, 743, 1035, 1177, 1361, 1560
BstNSI	RCATGY	1 cut(s)	433
BstSCI	CCNGG	9 cut(s)	201, 576, 741, 1033, 1175, 1359, 1558, 1585, 2001
BstSFI	CTRYAG	3 cut(s)	601, 793, 1960
BstSLI	GKGCMC	3 cut(s)	146, 1587, 1641
BstUI	CGCG	2 cut(s)	140, 2106
BstV1I	GCAGC	6 cut(s)	144, 484, 622, 749, 983, 1372
BstV2I	GAAGAC	3 cut(s)	60, 301, 1348
BstX2I	RGATCY	2 cut(s)	565, 1018
BstYI	RGATCY	2 cut(s)	565, 1018
BstZ17I	GTATAC	1 cut(s)	1732
Bsu15I	ATCGAT	1 cut(s)	15
BsuI	GTATCC	1 cut(s)	835
BsuRI	GGCC	7 cut(s)	74, 144, 198, 541, 576, 866, 1033
BsuTUI	ATCGAT	1 cut(s)	15
BtgI	CCRYGG	2 cut(s)	614, 1106
BtsCI	GGATG	8 cut(s)	400, 685, 1075, 1333, 1607, 1768, 1814, 2121
BtsI	GCAGTG	1 cut(s)	690
BtsIMutI	CAGTG	6 cut(s)	31, 297, 633, 690, 798, 987
Cac8I	GCNNGC	7 cut(s)	132, 136, 142, 782, 868, 1698, 1862
CciI	TCATGA	1 cut(s)	1543
CfoI	GCGC	2 cut(s)	140, 2106
Cfr13I	GGNCC	7 cut(s)	142, 143, 196, 864, 1173, 1556, 1605
ClaI	ATCGAT	1 cut(s)	15
CseI	GACGC	2 cut(s)	96, 98
Csp6I	GTAC	2 cut(s)	850, 2079
CviAII	CATG	9 cut(s)	284, 430, 572, 1091, 1107, 1544, 1612, 1853, 1880
CviQI	GTAC	2 cut(s)	850, 2079
DdeI	CTNAG	5 cut(s)	288, 315, 695, 716, 1425
DpnI	GATC	9 cut(s)	18, 240, 567, 918, 1020, 1851, 1896, 1912, 2007
DpnII	GATC	9 cut(s)	16, 238, 565, 916, 1018, 1849, 1894, 1910, 2005
DraIII	CACNNNGTG	1 cut(s)	639
DrdI	GACNNNNNNGTC	1 cut(s)	1997
DriI	GACNNNNNGTC	1 cut(s)	1562
DseDI	GACNNNNNNGTC	1 cut(s)	1997
Eam1104I	CTCTTC	3 cut(s)	260, 877, 1513
Eam1105I	GACNNNNNGTC	1 cut(s)	1562
EarI	CTCTTC	3 cut(s)	260, 877, 1513
EciI	GGCGGA	1 cut(s)	581
Ecl136II	GAGCTC	2 cut(s)	124, 1523
Eco130I	CCWWGG	3 cut(s)	519, 860, 1106
Eco147I	AGGCCT	1 cut(s)	541
Eco24I	GRGCYC	3 cut(s)	126, 146, 1525
Eco31I	GGTCTC	1 cut(s)	1406
Eco47I	GGWCC	3 cut(s)	1173, 1556, 1605
Eco53kI	GAGCTC	2 cut(s)	124, 1523
Eco57I	CTGAAG	4 cut(s)	984, 1362, 1822, 2043
EcoICRI	GAGCTC	2 cut(s)	124, 1523
EcoRI	GAATTC	2 cut(s)	878, 1718
EcoRII	CCWGG	7 cut(s)	201, 576, 741, 1033, 1175, 1359, 1558
EcoT14I	CCWWGG	3 cut(s)	519, 860, 1106
EcoT38I	GRGCYC	3 cut(s)	126, 146, 1525
ErhI	CCWWGG	3 cut(s)	519, 860, 1106
Esp3I	CGTCTC	1 cut(s)	676
FaeI	CATG	9 cut(s)	287, 433, 575, 1094, 1110, 1547, 1615, 1856, 1883
FaqI	GGGAC	2 cut(s)	184, 1985
FatI	CATG	9 cut(s)	283, 429, 571, 1090, 1106, 1543, 1611, 1852, 1879
FauI	CCCGC	3 cut(s)	133, 252, 1534
FauNDI	CATATG	2 cut(s)	1513, 1780
FbaI	TGATCA	1 cut(s)	916
FblI	GTMKAC	2 cut(s)	1731, 1963
Fnu4HI	GCNGC	7 cut(s)	158, 191, 498, 611, 738, 972, 1386
FokI	GGATG	8 cut(s)	407, 692, 1082, 1320, 1614, 1755, 1801, 2128
FriOI	GRGCYC	3 cut(s)	126, 146, 1525
Fsp4HI	GCNGC	7 cut(s)	158, 191, 498, 611, 738, 972, 1386
FspBI	CTAG	5 cut(s)	462, 722, 1055, 1137, 1316
GlaI	GCGC	2 cut(s)	139, 2105
GluI	GCNGC	7 cut(s)	158, 191, 498, 611, 738, 972, 1386
GsuI	CTGGAG	4 cut(s)	224, 599, 1581, 2043
HaeIII	GGCC	7 cut(s)	74, 144, 198, 541, 576, 866, 1033
HapII	CCGG	3 cut(s)	1368, 1587, 2003
HgaI	GACGC	2 cut(s)	96, 98
HhaI	GCGC	2 cut(s)	140, 2106
Hin1I	GRCGYC	1 cut(s)	107
Hin1II	CATG	9 cut(s)	287, 433, 575, 1094, 1110, 1547, 1615, 1856, 1883
Hin6I	GCGC	2 cut(s)	138, 2104
HinP1I	GCGC	2 cut(s)	138, 2104
HincII	GTYRAC	1 cut(s)	417
HindII	GTYRAC	1 cut(s)	417
HindIII	AAGCTT	1 cut(s)	278
HpaII	CCGG	3 cut(s)	1368, 1587, 2003
HphI	GGTGA	8 cut(s)	713, 796, 826, 1466, 1500, 1662, 1838, 1935
Hpy188III	TCNNGA	6 cut(s)	332, 1261, 1280, 1490, 1544, 1931
Hpy99I	CGWCG	6 cut(s)	34, 55, 67, 92, 112, 495
HpyAV	CCTTC	6 cut(s)	359, 552, 726, 900, 1258, 1797
HpyCH4III	ACNGT	6 cut(s)	26, 175, 802, 1400, 1410, 1799
HpyCH4IV	ACGT	1 cut(s)	1831
HpyF10VI	GCNNNNNNNGC	3 cut(s)	358, 619, 2018
HpyF3I	CTNAG	5 cut(s)	288, 315, 695, 716, 1425
HpySE526I	ACGT	1 cut(s)	1831
Hsp92I	GRCGYC	1 cut(s)	107
Hsp92II	CATG	9 cut(s)	287, 433, 575, 1094, 1110, 1547, 1615, 1856, 1883
HspAI	GCGC	2 cut(s)	138, 2104
Ksp22I	TGATCA	1 cut(s)	916
Kzo9I	GATC	9 cut(s)	16, 238, 565, 916, 1018, 1849, 1894, 1910, 2005
LguI	GCTCTTC	2 cut(s)	877, 1513
LmnI	GCTCC	5 cut(s)	121, 205, 909, 1229, 1528
Lsp1109I	GCAGC	6 cut(s)	144, 484, 622, 749, 983, 1372
LweI	GCATC	6 cut(s)	367, 670, 958, 1241, 1342, 1605
MaeI	CTAG	5 cut(s)	462, 722, 1055, 1137, 1316
MaeII	ACGT	1 cut(s)	1831
MaeIII	GTNAC	6 cut(s)	701, 802, 1394, 1410, 1711, 1832
MalI	GATC	9 cut(s)	18, 240, 567, 918, 1020, 1851, 1896, 1912, 2007
MboI	GATC	9 cut(s)	16, 238, 565, 916, 1018, 1849, 1894, 1910, 2005
MfeI	CAATTG	1 cut(s)	785
MflI	RGATCY	2 cut(s)	565, 1018
MhlI	GDGCHC	6 cut(s)	126, 146, 354, 1525, 1587, 1641
MlyI	GAGTC	4 cut(s)	299, 1362, 1572, 1959
MmeI	TCCRAC	6 cut(s)	111, 248, 791, 1429, 1796, 2047
MroXI	GAANNNNTTC	1 cut(s)	1497
MseI	TTAA	4 cut(s)	747, 843, 1253, 1685
MslI	CAYNNNNRTG	4 cut(s)	809, 1548, 1613, 1655
MspA1I	CMGCKG	1 cut(s)	1311
MspI	CCGG	3 cut(s)	1368, 1587, 2003
MspR9I	CCNGG	9 cut(s)	203, 578, 743, 1035, 1177, 1361, 1560, 1587, 2003
MunI	CAATTG	1 cut(s)	785
Mva1269I	GAATGC	3 cut(s)	823, 841, 1864
MvaI	CCWGG	7 cut(s)	203, 578, 743, 1035, 1177, 1361, 1560
MvnI	CGCG	2 cut(s)	140, 2106
MwoI	GCNNNNNNNGC	3 cut(s)	358, 619, 2018
NciI	CCSGG	2 cut(s)	1587, 2003
NcoI	CCATGG	1 cut(s)	1106
NdeI	CATATG	2 cut(s)	1513, 1780
NdeII	GATC	9 cut(s)	16, 238, 565, 916, 1018, 1849, 1894, 1910, 2005
NlaIII	CATG	9 cut(s)	287, 433, 575, 1094, 1110, 1547, 1615, 1856, 1883
NlaIV	GGNNCC	7 cut(s)	144, 590, 911, 1020, 1259, 1366, 1584
NmeAIII	GCCGAG	2 cut(s)	209, 526
NmuCI	GTSAC	3 cut(s)	701, 802, 1410
NspI	RCATGY	1 cut(s)	433
OliI	CACNNNNGTG	2 cut(s)	809, 1655
PagI	TCATGA	1 cut(s)	1543
PceI	AGGCCT	1 cut(s)	541
PciI	ACATGT	1 cut(s)	429
PciSI	GCTCTTC	2 cut(s)	877, 1513
PcsI	WCGNNNNNNNCGW	2 cut(s)	59, 221
PctI	GAATGC	3 cut(s)	823, 841, 1864
PdmI	GAANNNNTTC	1 cut(s)	1497
PfeI	GAWTC	6 cut(s)	1247, 1327, 1421, 1457, 1467, 1934
PflFI	GACNNNGTC	1 cut(s)	305
PflMI	CCANNNNNTGG	1 cut(s)	639
PfoI	TCCNGGA	2 cut(s)	1175, 2001
PkrI	GCNGC	7 cut(s)	159, 192, 499, 612, 739, 973, 1387
PleI	GAGTC	4 cut(s)	298, 1361, 1571, 1959
PpsI	GAGTC	4 cut(s)	298, 1361, 1571, 1959
PscI	ACATGT	1 cut(s)	429
PsiI	TTATAA	1 cut(s)	1904
Psp124BI	GAGCTC	2 cut(s)	126, 1525
Psp6I	CCWGG	7 cut(s)	201, 576, 741, 1033, 1175, 1359, 1558
PspGI	CCWGG	7 cut(s)	201, 576, 741, 1033, 1175, 1359, 1558
PspN4I	GGNNCC	7 cut(s)	144, 590, 911, 1020, 1259, 1366, 1584
PspOMI	GGGCCC	1 cut(s)	142
PspPI	GGNCC	7 cut(s)	142, 143, 196, 864, 1173, 1556, 1605
PsuI	RGATCY	2 cut(s)	565, 1018
PsyI	GACNNNGTC	1 cut(s)	305
RsaI	GTAC	2 cut(s)	851, 2080
RsaNI	GTAC	2 cut(s)	850, 2079
RseI	CAYNNNNRTG	4 cut(s)	809, 1548, 1613, 1655
SacI	GAGCTC	2 cut(s)	126, 1525
SapI	GCTCTTC	2 cut(s)	877, 1513
SaqAI	TTAA	4 cut(s)	747, 843, 1253, 1685
SatI	GCNGC	7 cut(s)	158, 191, 498, 611, 738, 972, 1386
Sau3AI	GATC	9 cut(s)	16, 238, 565, 916, 1018, 1849, 1894, 1910, 2005
Sau96I	GGNCC	7 cut(s)	142, 143, 196, 864, 1173, 1556, 1605
SchI	GAGTC	4 cut(s)	299, 1362, 1572, 1959
ScrFI	CCNGG	9 cut(s)	203, 578, 743, 1035, 1177, 1361, 1560, 1587, 2003
SduI	GDGCHC	6 cut(s)	126, 146, 354, 1525, 1587, 1641
SfaNI	GCATC	6 cut(s)	367, 670, 958, 1241, 1342, 1605
SfcI	CTRYAG	3 cut(s)	601, 793, 1960
SinI	GGWCC	3 cut(s)	1173, 1556, 1605
SmiMI	CAYNNNNRTG	4 cut(s)	809, 1548, 1613, 1655
SseBI	AGGCCT	1 cut(s)	541
SspI	AATATT	1 cut(s)	1497
SspMI	CTAG	5 cut(s)	462, 722, 1055, 1137, 1316
SstI	GAGCTC	2 cut(s)	126, 1525
StuI	AGGCCT	1 cut(s)	541
StyD4I	CCNGG	9 cut(s)	201, 576, 741, 1033, 1175, 1359, 1558, 1585, 2001
StyI	CCWWGG	3 cut(s)	519, 860, 1106
TaaI	ACNGT	6 cut(s)	26, 175, 802, 1400, 1410, 1799
TaiI	ACGT	1 cut(s)	1834
TaqI	TCGA	2 cut(s)	15, 1909
TatI	WGTACW	2 cut(s)	849, 2078
TauI	GCSGC	1 cut(s)	193
TfiI	GAWTC	6 cut(s)	1247, 1327, 1421, 1457, 1467, 1934
Tru1I	TTAA	4 cut(s)	747, 843, 1253, 1685
Tru9I	TTAA	4 cut(s)	747, 843, 1253, 1685
TscAI	CASTG	6 cut(s)	31, 304, 640, 690, 805, 994
TseFI	GTSAC	3 cut(s)	701, 802, 1410
TseI	GCWGC	6 cut(s)	157, 497, 610, 737, 971, 1385
Tsp45I	GTSAC	3 cut(s)	701, 802, 1410
TspDTI	ATGAA	6 cut(s)	90, 272, 871, 1560, 1656, 2024
TspGWI	ACGGA	4 cut(s)	47, 486, 631, 1730
TspRI	CASTG	6 cut(s)	31, 304, 640, 690, 805, 994
Tth111I	GACNNNGTC	1 cut(s)	305
Van91I	CCANNNNNTGG	1 cut(s)	639
VneI	GTGCAC	1 cut(s)	1637
VpaK11BI	GGWCC	3 cut(s)	1173, 1556, 1605
XapI	RAATTY	4 cut(s)	878, 1205, 1284, 1718
XceI	RCATGY	1 cut(s)	433
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1367
XmiI	GTMKAC	2 cut(s)	1731, 1963
XmnI	GAANNNNTTC	1 cut(s)	1497
XspI	CTAG	5 cut(s)	462, 722, 1055, 1137, 1316

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.