Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr05

Orthogroup ID

OG0013140

Physical Location & Seq

Genomic Coordinates Reverse (-)

46650689 .. 46654777

Gene Structure 4089 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD05G1347900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1779 bp

ATGGCCAAATCGTCGGCGAGCTCGACGACATCGTTCCCGCCGCTCAAGCCCGACGACTACTCCCACAGCCCCGTGCACTACGCCGTCGTTTTGTCAGATCACACCACACTCTCCCGAATCGTCGCCACACTCCCCCGACTTGCCGATCCGACTCAGATCCACACGGAGTCCGACTCCCTCTCCCAGGAACGAACCGCGGACCGGATCTCGTCCGTGCTCGACCGCCGCGACGTTCCCTACAGAGAAACCCCTCTCCACCTCGCCGTCCGACTAAACAACGCTGTCTCCGCCAAAATTTTAGCCTCCGCCGGCGCCGACGTGTCACTCCAGAACTCCGCAGGCTGGAATCCCTTACAGGAAGCATTGTGTCGCCGGAGCTCCGACGTCGCTTTAATCCTTCTCCGGCTCCACCACCGTTCCGCTTGGGCTAAGTGGCGCCGCCGCCTGCCACGTGCAATCGCCGTGCTCCGGAGAATGCGGGATTTTTACATGGAGATCTCGTTCCACTTCGAGAGCTCCGTCATTCCCTTCGTCGGAAAGATCGCTCCCTCCGACACGTACAAGATCTGGAAGCGCGACGGCAATTTGCGCGCAGACACGTCGCTGGCCGGATTCGACGGATTGAAGATCCAGCGCGCCGATCAGAGCTTCCTATTCCTCGGGGACGGCGATCAGAGCCACGACGTTCCCCCGGGATCGCTGCTCGTCTTGAACCGCGACGAACGGAAAATCTTCGACGCGTTCGAGAACGCCGGGTCGCCGATGAGCGAGTCGGACATCGCCGGGTTCTGCGCCCAGACGAGCGTGTACCGCCCGGGCATGGACGTCACCAGGGCGGAGCTGGTCGGGAGGACCAATTGGAGGAGGCAGGAGAAGACGGAGAGCGTCGGAGAGTGGAAAGCTCGAGTGTACGAAATTCACAACGTTGTGTTCAGCTTCCGCTCGAGAAAAGTCACCAATGGCGATGCTGACGTGGCGGGGAGCGAGCAGGTGCTTCCTCTTGAGCTCGACGAGGACGACGACGGGTTTTTGGTGGCGGAGAATCCGAGTTTCGGAATTCCCGACGGCCGGAGGCACAGTAGCTTCGTGAGGGAGGAGAGGGAGTTCGTGTCATTGGGGAGAAAAAGCGTCGACGTTTCGTCAATTTCCGGGCCGCCGTCGAGGAGGTGTAAAACGGCCACTATTGTGGCGGCACCAGTGCAGACGAAAGAGAAAGAGTTCGTGAGGAGCTTGAGGCCGTGCGTTTGGCTGACGGAGCAGTTTCCGTTGAAGACGGAGGAGCTGCTGCCGTTACTGGACATCCTGGCGAACAAGGTGAAGGCCGTTAGGAGGATGAGGGAGTTGCTCACCACCAAGTTCCCGCCAGGGACTTTTCCGGTTAAGGTGGCAATACCGGTGGTCCCCACGGTGAGAGTGGTGATAACATTCACAAAGTTCGTTGAGCTTCCGCCAACTGAGCAATTCTTCACTCCAATGTCAAGCCCCAGACAGTTTCTTCATGAAGGGCGAGGCCAACAAGAGGAGGAACACAAATCCAAGCCACAAAAACCATCCTCCTTTTCGTCGTCGTCGTCGTGGCTGAGGCGGAGCAGCAGCCAGTCGGGGTCTTCCGTGAGCAAGCAGAACCAACACCAACACTGTGCTCCCCCGGCAGTGTCTGCAGCGCAAGACTCTTCTGACCCTTTCGCCATTCCTAGCGGTTATACGTGGACGAGCATCGATGACAAGTCTCGCAAAATGAAGAAATCCAAGTCCAGCAGGAGATCAATATCCAACTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005102 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0006621 GO:0008104 GO:0008150 GO:0009889 GO:0009987 GO:0010556 GO:0010869 GO:0010941 GO:0012505 GO:0019222 GO:0031323 GO:0032507 GO:0033036 GO:0033365 GO:0034613 GO:0035437 GO:0042981 GO:0043067 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0045185 GO:0048471 GO:0050789 GO:0050794 GO:0051179 GO:0051235 GO:0051641 GO:0051651 GO:0060255 GO:0065007 GO:0065008 GO:0070727 GO:0070972 GO:0072595 GO:2000209

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Ank_2 Ank_4 GPCR_chapero_1

Protein Analysis

593

Amino Acids

66.23

Weight (kDa)

9.48

Isoelectric Point (pI)

60.9

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Ank_2	PF12796	80 - 134	6.3e-06	Ankyrin repeats (3 copies)
ANKRD13_C	PF11904	197 - 569	9.7e-103	ANKRD13 C-terminal

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0013140)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G62050
fragaria_vesca	FvH4_3g01950
malus_domestica	MD05G1347900.v1.1 MD10G1322300.v1.1
prunus_persica	Prupe.4G019600_v2.0.a1
pyrus_communis	pycom05g31540 pycom10g27280
rosa_chinensis	RchiOBHm_Chr5g0002861
rosa_laevigata	RLG00000031066
rosa_multiflora	Rmu_sc0013603.1_g000009 Rmu_sc0020722.1_g000002
rosa_roxburghii	Rroxscaffold_1G00072990
rosa_rugosa	Rorug04G0400300
rosa_samantha	Rh5AG023200 Rh5BG025900 Rh5CG025800 Rh5DG024300
rosa_wichuraiana	Rw5G002160

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	979
AatII	GACGTC	2 cut(s)	387, 828
Acc36I	ACCTGC	1 cut(s)	979
AccB1I	GGYRCC	3 cut(s)	311, 435, 1192
AccBSI	CCGCTC	2 cut(s)	43, 942
AccI	GTMKAC	1 cut(s)	1131
AccII	CGCG	7 cut(s)	197, 228, 576, 591, 636, 717, 740
AccIII	TCCGGA	1 cut(s)	468
AclI	AACGTT	1 cut(s)	924
AclWI	GGATC	5 cut(s)	140, 151, 212, 622, 703
AcoI	YGGCCR	4 cut(s)	3, 606, 1066, 1176
AcsI	RAATTY	3 cut(s)	294, 915, 1056
AcvI	CACGTG	1 cut(s)	452
AcyI	GRCGYC	4 cut(s)	312, 384, 436, 825
AfaI	GTAC	3 cut(s)	560, 809, 911
AflIII	ACRYGT	4 cut(s)	318, 555, 597, 738
AgeI	ACCGGT	1 cut(s)	1393
AgsI	TTSAA	3 cut(s)	625, 712, 1270
AhdI	GACNNNNNGTC	1 cut(s)	1138
AjiI	CACGTC	3 cut(s)	319, 600, 973
AjnI	CCWGG	4 cut(s)	183, 830, 1302, 1363
AleI	CACNNNNGTG	1 cut(s)	1184
AloI	GAACNNNNNNTCC	4 cut(s)	485, 517, 1088, 1120
Alw21I	GWGCWC	8 cut(s)	23, 78, 219, 380, 468, 518, 1008, 1645
Alw26I	GTCTC	2 cut(s)	289, 1734
Alw44I	GTGCAC	1 cut(s)	74
AlwI	GGATC	5 cut(s)	140, 151, 212, 622, 703
AlwNI	CAGNNNCTG	1 cut(s)	1658
Ama87I	CYCGRG	5 cut(s)	659, 691, 814, 903, 943
Aor13HI	TCCGGA	1 cut(s)	468
AoxI	GGCC	8 cut(s)	3, 606, 1066, 1151, 1176, 1235, 1320, 1510
ApaLI	GTGCAC	1 cut(s)	74
ApeKI	GCWGC	6 cut(s)	700, 1282, 1285, 1590, 1593, 1661
ApoI	RAATTY	3 cut(s)	294, 915, 1056
AsiGI	ACCGGT	1 cut(s)	1393
Asp700I	GAANNNNTTC	2 cut(s)	731, 1217
AspLEI	GCGC	9 cut(s)	314, 438, 576, 591, 593, 636, 638, 794, 1666
AspS9I	GGNCC	4 cut(s)	199, 852, 1151, 1399
AsuC2I	CCSGG	8 cut(s)	692, 693, 754, 784, 815, 816, 1150, 1649
AsuHPI	GGTGA	6 cut(s)	820, 946, 1327, 1339, 1420, 1429
AvaI	CYCGRG	5 cut(s)	659, 691, 814, 903, 943
AvaII	GGWCC	3 cut(s)	199, 852, 1399
BaeGI	GKGCMC	1 cut(s)	78
BalI	TGGCCA	1 cut(s)	5
BanI	GGYRCC	3 cut(s)	311, 435, 1192
BanII	GRGCYC	4 cut(s)	23, 380, 518, 1008
BbrPI	CACGTG	1 cut(s)	452
BbsI	GAAGAC	3 cut(s)	881, 1277, 1599
Bbv12I	GWGCWC	8 cut(s)	23, 78, 219, 380, 468, 518, 1008, 1645
BbvCI	CCTCAGC	1 cut(s)	1580
BbvI	GCAGC	6 cut(s)	687, 1269, 1272, 1602, 1605, 1673
BccI	CCATC	1 cut(s)	1558
BcgI	CGANNNNNNTGC	2 cut(s)	582, 616
BciT130I	CCWGG	4 cut(s)	185, 832, 1304, 1365
BcnI	CCSGG	8 cut(s)	692, 693, 754, 784, 815, 816, 1150, 1649
BcoDI	GTCTC	2 cut(s)	289, 1734
BfaI	CTAG	1 cut(s)	1695
BfmI	CTRYAG	2 cut(s)	238, 1659
BfoI	RGCGCY	2 cut(s)	315, 439
BfuAI	ACCTGC	1 cut(s)	979
BglII	AGATCT	2 cut(s)	495, 564
Bme18I	GGWCC	3 cut(s)	199, 852, 1399
BmeRI	GACNNNNNGTC	1 cut(s)	1138
BmeT110I	CYCGRG	5 cut(s)	659, 691, 814, 903, 943
BmgBI	CACGTC	3 cut(s)	319, 600, 973
BmgT120I	GGNCC	4 cut(s)	199, 852, 1151, 1399
BmiI	GGNNCC	5 cut(s)	313, 407, 437, 1194, 1401
BmsI	GCATC	2 cut(s)	955, 1725
BpiI	GAAGAC	3 cut(s)	881, 1277, 1599
BplI	GAGNNNNNCTC	2 cut(s)	158, 190
BpmI	CTGGAG	1 cut(s)	311
Bpu10I	CCTNAGC	1 cut(s)	1580
BpuEI	CTTGAG	3 cut(s)	29, 1022, 1252
BpuMI	CCSGG	8 cut(s)	692, 693, 754, 784, 815, 816, 1150, 1649
Bsa29I	ATCGAT	1 cut(s)	1719
BsaAI	YACGTR	3 cut(s)	452, 558, 1707
BsaBI	GATNNNNATC	1 cut(s)	1768
BsaHI	GRCGYC	4 cut(s)	312, 384, 436, 825
BsaWI	WCCGGW	4 cut(s)	201, 468, 1375, 1393
Bse118I	RCCGGY	2 cut(s)	308, 1393
Bse1I	ACTGG	3 cut(s)	1196, 1299, 1597
Bse8I	GATNNNNATC	1 cut(s)	1768
BseAI	TCCGGA	1 cut(s)	468
BseBI	CCWGG	4 cut(s)	185, 832, 1304, 1365
BseCI	ATCGAT	1 cut(s)	1719
BseGI	GGATG	3 cut(s)	1299, 1338, 1550
BseJI	GATNNNNATC	1 cut(s)	1768
BseMII	CTCAG	3 cut(s)	167, 1446, 1571
BseNI	ACTGG	3 cut(s)	1196, 1299, 1597
BsePI	GCGCGC	2 cut(s)	589, 634
BseRI	GAGGAG	6 cut(s)	877, 1109, 1177, 1240, 1292, 1535
BseSI	GKGCMC	1 cut(s)	78
BseX3I	CGGCCG	1 cut(s)	1066
BseXI	GCAGC	3 cut(s)	687, 1269, 1272
BsgI	GTGCAG	1 cut(s)	1220
Bsh1236I	CGCG	7 cut(s)	197, 228, 576, 591, 636, 717, 740
Bsh1285I	CGRYCG	2 cut(s)	223, 1069
BshFI	GGCC	8 cut(s)	5, 608, 1068, 1153, 1178, 1237, 1322, 1512
BshNI	GGYRCC	3 cut(s)	311, 435, 1192
BshTI	ACCGGT	1 cut(s)	1393
BshVI	ATCGAT	1 cut(s)	1719
BsiEI	CGRYCG	2 cut(s)	223, 1069
BsiHKAI	GWGCWC	8 cut(s)	23, 78, 219, 380, 468, 518, 1008, 1645
BsiHKCI	CYCGRG	5 cut(s)	659, 691, 814, 903, 943
BslFI	GGGAC	3 cut(s)	677, 1381, 1385
BsmAI	GTCTC	2 cut(s)	289, 1734
BsmBI	CGTCTC	3 cut(s)	92, 202, 714
BsmFI	GGGAC	3 cut(s)	677, 1381, 1385
BsmI	GAATGC	1 cut(s)	480
BsnI	GGCC	8 cut(s)	5, 608, 1068, 1153, 1178, 1237, 1322, 1512
BsoBI	CYCGRG	5 cut(s)	659, 691, 814, 903, 943
Bsp1286I	GDGCHC	8 cut(s)	23, 78, 219, 380, 468, 518, 1008, 1645
Bsp13I	TCCGGA	1 cut(s)	468
BspANI	GGCC	8 cut(s)	5, 608, 1068, 1153, 1178, 1237, 1322, 1512
BspCNI	CTCAG	3 cut(s)	166, 1447, 1572
BspDI	ATCGAT	1 cut(s)	1719
BspEI	TCCGGA	1 cut(s)	468
BspFNI	CGCG	7 cut(s)	197, 228, 576, 591, 636, 717, 740
BspHI	TCATGA	1 cut(s)	1498
BspLI	GGNNCC	5 cut(s)	313, 407, 437, 1194, 1401
BspMAI	CTGCAG	1 cut(s)	1663
BspMI	ACCTGC	1 cut(s)	979
BspPI	GGATC	5 cut(s)	140, 151, 212, 622, 703
BspT107I	GGYRCC	3 cut(s)	311, 435, 1192
BsrBI	CCGCTC	2 cut(s)	43, 942
BsrFI	RCCGGY	2 cut(s)	308, 1393
BsrI	ACTGG	3 cut(s)	1196, 1299, 1597
BssAI	RCCGGY	2 cut(s)	308, 1393
BssHII	GCGCGC	2 cut(s)	589, 634
BssNI	GRCGYC	4 cut(s)	312, 384, 436, 825
Bst2UI	CCWGG	4 cut(s)	185, 832, 1304, 1365
Bst4CI	ACNGT	5 cut(s)	416, 1079, 1408, 1491, 1640
Bst6I	CTCTTC	1 cut(s)	1678
BstACI	GRCGYC	4 cut(s)	312, 384, 436, 825
BstAPI	GCANNNNNTGC	1 cut(s)	1658
BstBAI	YACGTR	3 cut(s)	452, 558, 1707
BstC8I	GCNNGC	9 cut(s)	19, 310, 340, 446, 591, 606, 636, 986, 1619
BstDEI	CTNAG	4 cut(s)	153, 429, 1455, 1580
BstDSI	CCRYGG	2 cut(s)	195, 1404
BstENI	CCTNNNNNAGG	1 cut(s)	182
BstF5I	GGATG	3 cut(s)	1299, 1338, 1550
BstFNI	CGCG	7 cut(s)	197, 228, 576, 591, 636, 717, 740
BstH2I	RGCGCY	2 cut(s)	315, 439
BstHHI	GCGC	9 cut(s)	314, 438, 576, 591, 593, 636, 638, 794, 1666
BstMAI	GTCTC	2 cut(s)	289, 1734
BstMCI	CGRYCG	2 cut(s)	223, 1069
BstNI	CCWGG	4 cut(s)	185, 832, 1304, 1365
BstSFI	CTRYAG	2 cut(s)	238, 1659
BstSLI	GKGCMC	1 cut(s)	78
BstUI	CGCG	7 cut(s)	197, 228, 576, 591, 636, 717, 740
BstV1I	GCAGC	6 cut(s)	687, 1269, 1272, 1602, 1605, 1673
BstV2I	GAAGAC	3 cut(s)	881, 1277, 1599
BstX2I	RGATCY	5 cut(s)	156, 204, 495, 564, 627
BstXI	CCANNNNNNTGG	1 cut(s)	1186
BstYI	RGATCY	5 cut(s)	156, 204, 495, 564, 627
BstZI	CGGCCG	1 cut(s)	1066
Bsu15I	ATCGAT	1 cut(s)	1719
BsuRI	GGCC	8 cut(s)	5, 608, 1068, 1153, 1178, 1237, 1322, 1512
BsuTUI	ATCGAT	1 cut(s)	1719
BtgI	CCRYGG	2 cut(s)	195, 1404
BtgZI	GCGATG	2 cut(s)	763, 978
BtrI	CACGTC	3 cut(s)	319, 600, 973
BtsCI	GGATG	3 cut(s)	1299, 1338, 1550
BtsI	GCAGTG	1 cut(s)	1659
BtsIMutI	CAGTG	3 cut(s)	1203, 1636, 1659
BveI	ACCTGC	1 cut(s)	979
Cac8I	GCNNGC	9 cut(s)	19, 310, 340, 446, 591, 606, 636, 986, 1619
CaiI	CAGNNNCTG	1 cut(s)	1658
CciI	TCATGA	1 cut(s)	1498
CfoI	GCGC	9 cut(s)	314, 438, 576, 591, 593, 636, 638, 794, 1666
Cfr10I	RCCGGY	2 cut(s)	308, 1393
Cfr13I	GGNCC	4 cut(s)	199, 852, 1151, 1399
Cfr42I	CCGCGG	1 cut(s)	198
Cfr9I	CCCGGG	2 cut(s)	691, 814
ClaI	ATCGAT	1 cut(s)	1719
CpoI	CGGWCCG	1 cut(s)	199
CseI	GACGC	3 cut(s)	746, 874, 1117
Csp6I	GTAC	3 cut(s)	559, 808, 910
CspAI	ACCGGT	1 cut(s)	1393
CspCI	CAANNNNNGTGG	2 cut(s)	1377, 1412
CspI	CGGWCCG	1 cut(s)	199
CviAII	CATG	3 cut(s)	490, 820, 1499
CviQI	GTAC	3 cut(s)	559, 808, 910
DdeI	CTNAG	4 cut(s)	153, 429, 1455, 1580
DinI	GGCGCC	2 cut(s)	313, 437
DriI	GACNNNNNGTC	1 cut(s)	1138
EaeI	YGGCCR	4 cut(s)	3, 606, 1066, 1176
EagI	CGGCCG	1 cut(s)	1066
Eam1104I	CTCTTC	1 cut(s)	1678
Eam1105I	GACNNNNNGTC	1 cut(s)	1138
EarI	CTCTTC	1 cut(s)	1678
EciI	GGCGGA	6 cut(s)	277, 295, 851, 1052, 1437, 1600
Ecl136II	GAGCTC	4 cut(s)	21, 378, 516, 1006
EclXI	CGGCCG	1 cut(s)	1066
Eco24I	GRGCYC	4 cut(s)	23, 380, 518, 1008
Eco47I	GGWCC	3 cut(s)	199, 852, 1399
Eco52I	CGGCCG	1 cut(s)	1066
Eco53kI	GAGCTC	4 cut(s)	21, 378, 516, 1006
Eco72I	CACGTG	1 cut(s)	452
Eco88I	CYCGRG	5 cut(s)	659, 691, 814, 903, 943
EcoICRI	GAGCTC	4 cut(s)	21, 378, 516, 1006
EcoNI	CCTNNNNNAGG	1 cut(s)	182
EcoRI	GAATTC	1 cut(s)	1056
EcoRII	CCWGG	4 cut(s)	183, 830, 1302, 1363
EcoT38I	GRGCYC	4 cut(s)	23, 380, 518, 1008
EgeI	GGCGCC	2 cut(s)	313, 437
EheI	GGCGCC	2 cut(s)	313, 437
FaeI	CATG	3 cut(s)	493, 823, 1502
FaiI	YATR	4 cut(s)	491, 821, 1500, 1704
FaqI	GGGAC	3 cut(s)	677, 1381, 1385
FatI	CATG	3 cut(s)	489, 819, 1498
FauI	CCCGC	4 cut(s)	45, 471, 970, 1368
FblI	GTMKAC	1 cut(s)	1131
FokI	GGATG	3 cut(s)	1286, 1345, 1537
FriOI	GRGCYC	4 cut(s)	23, 380, 518, 1008
FspBI	CTAG	1 cut(s)	1695
GlaI	GCGC	9 cut(s)	313, 437, 575, 590, 592, 635, 637, 793, 1665
GsuI	CTGGAG	1 cut(s)	311
HaeII	RGCGCY	2 cut(s)	315, 439
HaeIII	GGCC	8 cut(s)	5, 608, 1068, 1153, 1178, 1237, 1322, 1512
HgaI	GACGC	3 cut(s)	746, 874, 1117
HhaI	GCGC	9 cut(s)	314, 438, 576, 591, 593, 636, 638, 794, 1666
Hin1I	GRCGYC	4 cut(s)	312, 384, 436, 825
Hin1II	CATG	3 cut(s)	493, 823, 1502
Hin6I	GCGC	9 cut(s)	312, 436, 574, 589, 591, 634, 636, 792, 1664
HinP1I	GCGC	9 cut(s)	312, 436, 574, 589, 591, 634, 636, 792, 1664
HincII	GTYRAC	1 cut(s)	1132
HindII	GTYRAC	1 cut(s)	1132
HinfI	GANTC	9 cut(s)	117, 151, 167, 173, 346, 612, 770, 1042, 1670
HphI	GGTGA	6 cut(s)	820, 946, 1327, 1339, 1420, 1429
Hpy166II	GTNNAC	5 cut(s)	76, 808, 910, 1132, 1710
Hpy8I	GTNNAC	5 cut(s)	76, 808, 910, 1132, 1710
HpyAV	CCTTC	4 cut(s)	407, 538, 1312, 1496
HpyCH4III	ACNGT	5 cut(s)	416, 1079, 1408, 1491, 1640
HpyCH4V	TGCA	4 cut(s)	76, 455, 1201, 1661
HpyF3I	CTNAG	4 cut(s)	153, 429, 1455, 1580
Hsp92I	GRCGYC	4 cut(s)	312, 384, 436, 825
Hsp92II	CATG	3 cut(s)	493, 823, 1502
HspAI	GCGC	9 cut(s)	312, 436, 574, 589, 591, 634, 636, 792, 1664
KasI	GGCGCC	2 cut(s)	311, 435
Kpn2I	TCCGGA	1 cut(s)	468
KroI	GCCGGC	1 cut(s)	308
KroNI	GCCGGC	1 cut(s)	310
KspI	CCGCGG	1 cut(s)	198
Lsp1109I	GCAGC	6 cut(s)	687, 1269, 1272, 1602, 1605, 1673
LweI	GCATC	2 cut(s)	955, 1725
MaeI	CTAG	1 cut(s)	1695
MaeIII	GTNAC	4 cut(s)	321, 826, 952, 1290
MbiI	CCGCTC	2 cut(s)	43, 942
MboII	GAAGA	9 cut(s)	637, 724, 886, 1282, 1456, 1487, 1599, 1665, 1753
MfeI	CAATTG	1 cut(s)	856
MflI	RGATCY	5 cut(s)	156, 204, 495, 564, 627
MhlI	GDGCHC	8 cut(s)	23, 78, 219, 380, 468, 518, 1008, 1645
MlsI	TGGCCA	1 cut(s)	5
MluCI	AATT	7 cut(s)	294, 583, 856, 915, 1056, 1143, 1460
MluI	ACGCGT	1 cut(s)	738
MluNI	TGGCCA	1 cut(s)	5
Mly113I	GGCGCC	2 cut(s)	312, 436
MlyI	GAGTC	5 cut(s)	145, 167, 176, 779, 1664
MmeI	TCCRAC	8 cut(s)	173, 195, 292, 405, 514, 576, 753, 868
Mox20I	TGGCCA	1 cut(s)	5
MreI	CGCCGGCG	1 cut(s)	308
MroI	TCCGGA	1 cut(s)	468
MroNI	GCCGGC	1 cut(s)	308
MroXI	GAANNNNTTC	2 cut(s)	731, 1217
MscI	TGGCCA	1 cut(s)	5
MseI	TTAA	2 cut(s)	392, 1380
MslI	CAYNNNNRTG	2 cut(s)	1184, 1472
Msp20I	TGGCCA	1 cut(s)	5
MspA1I	CMGCKG	1 cut(s)	197
MunI	CAATTG	1 cut(s)	856
Mva1269I	GAATGC	1 cut(s)	480
MvaI	CCWGG	4 cut(s)	185, 832, 1304, 1365
MvnI	CGCG	7 cut(s)	197, 228, 576, 591, 636, 717, 740
NaeI	GCCGGC	1 cut(s)	310
NarI	GGCGCC	2 cut(s)	312, 436
NciI	CCSGG	8 cut(s)	692, 693, 754, 784, 815, 816, 1150, 1649
NgoMIV	GCCGGC	1 cut(s)	308
NlaIII	CATG	3 cut(s)	493, 823, 1502
NlaIV	GGNNCC	5 cut(s)	313, 407, 437, 1194, 1401
NmuCI	GTSAC	3 cut(s)	321, 826, 952
OliI	CACNNNNGTG	1 cut(s)	1184
PaeR7I	CTCGAG	2 cut(s)	903, 943
PagI	TCATGA	1 cut(s)	1498
PaqCI	CACCTGC	1 cut(s)	979
PauI	GCGCGC	2 cut(s)	589, 634
PctI	GAATGC	1 cut(s)	480
PdiI	GCCGGC	1 cut(s)	310
PdmI	GAANNNNTTC	2 cut(s)	731, 1217
PfeI	GAWTC	4 cut(s)	117, 346, 612, 1042
PinAI	ACCGGT	1 cut(s)	1393
PleI	GAGTC	4 cut(s)	145, 167, 175, 778
PluTI	GGCGCC	2 cut(s)	315, 439
PmaCI	CACGTG	1 cut(s)	452
PmlI	CACGTG	1 cut(s)	452
PpsI	GAGTC	5 cut(s)	145, 167, 175, 778, 1664
Ppu21I	YACGTR	3 cut(s)	452, 558, 1707
Psp124BI	GAGCTC	4 cut(s)	23, 380, 518, 1008
Psp1406I	AACGTT	1 cut(s)	924
Psp6I	CCWGG	4 cut(s)	183, 830, 1302, 1363
PspCI	CACGTG	1 cut(s)	452
PspGI	CCWGG	4 cut(s)	183, 830, 1302, 1363
PspN4I	GGNNCC	5 cut(s)	313, 407, 437, 1194, 1401
PspPI	GGNCC	4 cut(s)	199, 852, 1151, 1399
PspXI	VCTCGAGB	1 cut(s)	903
PstI	CTGCAG	1 cut(s)	1663
PstNI	CAGNNNCTG	1 cut(s)	1658
PsuI	RGATCY	5 cut(s)	156, 204, 495, 564, 627
PteI	GCGCGC	2 cut(s)	589, 634
RsaI	GTAC	3 cut(s)	560, 809, 911
RsaNI	GTAC	3 cut(s)	559, 808, 910
RseI	CAYNNNNRTG	2 cut(s)	1184, 1472
Rsr2I	CGGWCCG	1 cut(s)	199
RsrII	CGGWCCG	1 cut(s)	199
SacI	GAGCTC	4 cut(s)	23, 380, 518, 1008
SacII	CCGCGG	1 cut(s)	198
SalI	GTCGAC	1 cut(s)	1130
SaqAI	TTAA	2 cut(s)	392, 1380
Sau96I	GGNCC	4 cut(s)	199, 852, 1151, 1399
SchI	GAGTC	5 cut(s)	145, 167, 176, 779, 1664
SduI	GDGCHC	8 cut(s)	23, 78, 219, 380, 468, 518, 1008, 1645
SfaNI	GCATC	2 cut(s)	955, 1725
SfcI	CTRYAG	2 cut(s)	238, 1659
SfoI	GGCGCC	2 cut(s)	313, 437
Sfr274I	CTCGAG	2 cut(s)	903, 943
Sfr303I	CCGCGG	1 cut(s)	198
SgrAI	CRCCGGYG	1 cut(s)	308
SgrBI	CCGCGG	1 cut(s)	198
SgrDI	CGTCGACG	1 cut(s)	1130
SinI	GGWCC	3 cut(s)	199, 852, 1399
SlaI	CTCGAG	2 cut(s)	903, 943
SmaI	CCCGGG	2 cut(s)	693, 816
SmiMI	CAYNNNNRTG	2 cut(s)	1184, 1472
SmlI	CTYRAG	5 cut(s)	44, 903, 943, 1001, 1231
SmoI	CTYRAG	5 cut(s)	44, 903, 943, 1001, 1231
SrfI	GCCCGGGC	1 cut(s)	816
Sse9I	AATT	7 cut(s)	294, 583, 856, 915, 1056, 1143, 1460
SspDI	GGCGCC	2 cut(s)	311, 435
SspMI	CTAG	1 cut(s)	1695
SstI	GAGCTC	4 cut(s)	23, 380, 518, 1008
TaaI	ACNGT	5 cut(s)	416, 1079, 1408, 1491, 1640
TasI	AATT	7 cut(s)	294, 583, 856, 915, 1056, 1143, 1460
TauI	GCSGC	6 cut(s)	43, 228, 441, 444, 1156, 1193
TfiI	GAWTC	4 cut(s)	117, 346, 612, 1042
Tru1I	TTAA	2 cut(s)	392, 1380
Tru9I	TTAA	2 cut(s)	392, 1380
TscAI	CASTG	3 cut(s)	1203, 1643, 1659
TseFI	GTSAC	3 cut(s)	321, 826, 952
TseI	GCWGC	6 cut(s)	700, 1282, 1285, 1590, 1593, 1661
Tsp45I	GTSAC	3 cut(s)	321, 826, 952
TspDTI	ATGAA	3 cut(s)	1487, 1515, 1754
TspMI	CCCGGG	2 cut(s)	691, 814
TspRI	CASTG	3 cut(s)	1203, 1643, 1659
VneI	GTGCAC	1 cut(s)	74
VpaK11BI	GGWCC	3 cut(s)	199, 852, 1399
XagI	CCTNNNNNAGG	1 cut(s)	182
XapI	RAATTY	3 cut(s)	294, 915, 1056
XcmI	CCANNNNNNNNNTGG	2 cut(s)	838, 1411
XhoI	CTCGAG	2 cut(s)	903, 943
XmaI	CCCGGG	2 cut(s)	691, 814
XmiI	GTMKAC	1 cut(s)	1131
XmnI	GAANNNNTTC	2 cut(s)	731, 1217
XspI	CTAG	1 cut(s)	1695
ZraI	GACGTC	2 cut(s)	385, 826

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.