Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr10

Orthogroup ID

OG0013140

Physical Location & Seq

Genomic Coordinates Reverse (-)

28289225 .. 28291879

Gene Structure 2655 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom10g27280.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1692 bp

ATGGCCAAATCGTCGGCGACCTCGACGCCGTCGTTTCCGCCGCTCAAGGCCGACGACTACTCCCACAGCCCGGTACACTATGCCGTCGTTCTGGCTGACCACACCACGCTCTCCCGAATCGTCGCCACCCTTCCGCAACTCGCCGATCCGACTCAGATCCACACCGAGTCCGACTCCCTCTCTCAGGAACGGACCGCTGACCGGATCTCATCCGTGCTCGACCGCCGCGACGTTCCCTTCCGAGAAACCCCTCTCCACCTCGCCGTCCGGCTAAACGACGTAGCGTCCGCCAAAATTCTAGCCTCCGCAGGCGCCGACGTGTCCCTCCAGAACTCCGCCGGCTGGAATCCCCTGCAGGAAGCCTTATGTCGCCGGAGCTCCGACATTGCTTTAATCCTTCTCCGGCTCCACCACCGCTCCGCTTGGGCGAACTCCGTCATTCCCTTCGTCGGGAAGATTGCTCCCTCCGACACGTACAAGATCTGGAAGCGCGACGGCAACTTGCGCGCCGACACGTCGCTGGCCGGATTTGACGGATTGAAGATCCAGCGCGCCGATCAGAGCTTCCTCTTCCTCGGCGACGGCGATCAAAGCCACGACGTTCCCCCGGGATCGCTGCTCGTTTTGAACCGCGACGATCGGAAAATCTTCGACGCGTTCGAGAACGCTGGGTCGCCCATGAGTGAGTCGGACATCGCCGGATTCTGCTCCCAGACGAGTGTGTACCGCCCGGGCATGGACGTCACAAAAGCGGAGCTAGTCGGGCGGACCAATTGGAGGAGGCAGGATAAGACGGAGAGCGTCGGAGAGTGGAAAGCTCGAGTGTACGAAATTCACAATGTCGTGTTCAGCTTCCGCTCGAGAAAAGTCGCCAATGGCGACGCTGATGTGGCAGGGAGCGAGCAGGTGCTTCCTCTCGAGCTCGACGAGGACGACGACGGGTTTTTGGTGGCGGAGAATCCGAGCTTTGGAATTCCCGACGGCAGGAGGCACAGTAGCTTCGTGAGGGAGGAGAGGGAGTTCGTAGCATTGGGGAGAAAAAGCGTCGACGTTTCGTCAATTTCCGGGCCGCCGTCAAGGAGGTCCAAAACAGCTGCGATTGCGGCGGCGCCAGTGCAGACGAAAGAGAAAGAGTTCGTGAGGAGCTTGAGGCCGTGCGTTTGGCTGACGGAGCAGTTCCCGTTAAAGACGGAGGAGCTGCTGCCGTTACTGGACATCTTGGCGAACAAGGTGAAGGCCGTTAGGAGGATGAGGGAGTTGCTCACCACCAAGTTCCCGCCAGGGACTTTTCCGGTTAAGGTGGCAATACCGGTGGTCCCCACAGTGAGAGTGGTGATAACGTTCACAAAGTTCGTTGAGCTCCCGCCAACTGAGCAATTCTTCACTCCAATGTCAAGCCCCAGACAGTTTATTCATGAAGGACGAGGCCAGCAAGAAGAGGAACACAAGTACAAGGCGCAAAAACCACCATCCTTTTCGTCATCGTCGTGGCTGAGGCGGAACAGCAGCCAGTCGGGGTCTTCCGCGAGCAAGCAAAACCACCAACACCAACACCGTGCTCCTCCGGCAGTGACGGCGGCTCAAGACTCTTCTGACCCTTTCGCCATTCCTAGCGGTTATACCTGGACGAGCATCGAGGACAAGTCTCACAAAATGAAGAAATCCAAGTCCACGAGGAGATCATCCAAGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005102 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0006621 GO:0008104 GO:0008150 GO:0009889 GO:0009987 GO:0010556 GO:0010869 GO:0010941 GO:0012505 GO:0019222 GO:0031323 GO:0032507 GO:0033036 GO:0033365 GO:0034613 GO:0035437 GO:0042981 GO:0043067 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0045185 GO:0048471 GO:0050789 GO:0050794 GO:0051179 GO:0051235 GO:0051641 GO:0051651 GO:0060255 GO:0065007 GO:0065008 GO:0070727 GO:0070972 GO:0072595 GO:2000209

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Ank_2 Ank_4 GPCR_chapero_1

Protein Analysis

564

Amino Acids

62.46

Weight (kDa)

9.18

Isoelectric Point (pI)

60.09

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Ank_2	PF12796	78 - 134	3.1e-06	Ankyrin repeats (3 copies)
ANKRD13_C	PF11904	169 - 541	1.4e-103	ANKRD13 C-terminal

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0013140)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G62050
fragaria_vesca	FvH4_3g01950
malus_domestica	MD05G1347900.v1.1 MD10G1322300.v1.1
prunus_persica	Prupe.4G019600_v2.0.a1
pyrus_communis	pycom05g31540 pycom10g27280
rosa_chinensis	RchiOBHm_Chr5g0002861
rosa_laevigata	RLG00000031066
rosa_multiflora	Rmu_sc0013603.1_g000009 Rmu_sc0020722.1_g000002
rosa_roxburghii	Rroxscaffold_1G00072990
rosa_rugosa	Rorug04G0400300
rosa_samantha	Rh5AG023200 Rh5BG025900 Rh5CG025800 Rh5DG024300
rosa_wichuraiana	Rw5G002160

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	895
AatII	GACGTC	1 cut(s)	744
Acc36I	ACCTGC	1 cut(s)	895
AccB1I	GGYRCC	2 cut(s)	311, 1108
AccBSI	CCGCTC	3 cut(s)	43, 417, 858
AccI	GTMKAC	1 cut(s)	1047
AccII	CGCG	7 cut(s)	228, 492, 507, 552, 633, 656, 1526
AclI	AACGTT	1 cut(s)	1340
AclWI	GGATC	5 cut(s)	140, 151, 212, 538, 619
AcoI	YGGCCR	2 cut(s)	3, 522
AcsI	RAATTY	3 cut(s)	294, 831, 972
AcyI	GRCGYC	4 cut(s)	26, 312, 741, 1109
AfaI	GTAC	5 cut(s)	75, 476, 725, 827, 1451
AflIII	ACRYGT	4 cut(s)	318, 471, 513, 654
AgeI	ACCGGT	1 cut(s)	1309
AgsI	TTSAA	2 cut(s)	541, 628
AhdI	GACNNNNNGTC	2 cut(s)	283, 1054
AjiI	CACGTC	2 cut(s)	319, 516
AjnI	CCWGG	2 cut(s)	1279, 1622
AloI	GAACNNNNNNTCC	2 cut(s)	1004, 1036
Alw21I	GWGCWC	5 cut(s)	219, 380, 924, 1362, 1561
Alw26I	GTCTC	1 cut(s)	1650
AlwI	GGATC	5 cut(s)	140, 151, 212, 538, 619
Ama87I	CYCGRG	5 cut(s)	607, 730, 819, 859, 917
AoxI	GGCC	7 cut(s)	3, 48, 522, 1067, 1151, 1236, 1426
ApeKI	GCWGC	5 cut(s)	616, 1094, 1198, 1201, 1506
ApoI	RAATTY	3 cut(s)	294, 831, 972
AsiGI	ACCGGT	1 cut(s)	1309
Asp700I	GAANNNNTTC	2 cut(s)	647, 1133
AspLEI	GCGC	8 cut(s)	314, 492, 507, 509, 552, 554, 1111, 1459
AspS9I	GGNCC	5 cut(s)	192, 768, 1067, 1083, 1315
AsuC2I	CCSGG	6 cut(s)	71, 608, 609, 731, 732, 1066
AsuHPI	GGTGA	3 cut(s)	1243, 1255, 1345
AvaI	CYCGRG	5 cut(s)	607, 730, 819, 859, 917
AvaII	GGWCC	4 cut(s)	192, 768, 1083, 1315
BalI	TGGCCA	1 cut(s)	5
BanI	GGYRCC	2 cut(s)	311, 1108
BanII	GRGCYC	3 cut(s)	380, 924, 1362
BauI	CACGAG	1 cut(s)	1672
BbsI	GAAGAC	1 cut(s)	1512
Bbv12I	GWGCWC	5 cut(s)	219, 380, 924, 1362, 1561
BbvCI	CCTCAGC	1 cut(s)	1493
BbvI	GCAGC	5 cut(s)	603, 1081, 1185, 1188, 1518
BccI	CCATC	1 cut(s)	1477
BciT130I	CCWGG	2 cut(s)	1281, 1624
BcnI	CCSGG	6 cut(s)	71, 608, 609, 731, 732, 1066
BcoDI	GTCTC	1 cut(s)	1650
BfaI	CTAG	3 cut(s)	299, 758, 1611
BfmI	CTRYAG	1 cut(s)	353
BfoI	RGCGCY	2 cut(s)	315, 1112
BfuAI	ACCTGC	1 cut(s)	895
BglII	AGATCT	1 cut(s)	480
Bme1390I	CCNGG	8 cut(s)	71, 608, 609, 731, 732, 1066, 1281, 1624
Bme18I	GGWCC	4 cut(s)	192, 768, 1083, 1315
BmeRI	GACNNNNNGTC	2 cut(s)	283, 1054
BmeT110I	CYCGRG	5 cut(s)	607, 730, 819, 859, 917
BmgBI	CACGTC	2 cut(s)	319, 516
BmgT120I	GGNCC	5 cut(s)	192, 768, 1067, 1083, 1315
BmiI	GGNNCC	4 cut(s)	313, 407, 1110, 1317
BmrFI	CCNGG	8 cut(s)	71, 608, 609, 731, 732, 1066, 1281, 1624
BmsI	GCATC	1 cut(s)	1641
BpiI	GAAGAC	1 cut(s)	1512
BplI	GAGNNNNNCTC	2 cut(s)	158, 190
BpmI	CTGGAG	1 cut(s)	311
Bpu10I	CCTNAGC	1 cut(s)	1493
BpuEI	CTTGAG	3 cut(s)	29, 1168, 1566
BpuMI	CCSGG	6 cut(s)	71, 608, 609, 731, 732, 1066
BsaAI	YACGTR	1 cut(s)	474
BsaHI	GRCGYC	4 cut(s)	26, 312, 741, 1109
BsaJI	CCNNGG	5 cut(s)	574, 606, 607, 730, 1280
BsaWI	WCCGGW	3 cut(s)	201, 1291, 1309
BsaXI	ACNNNNNCTCC	8 cut(s)	44, 74, 401, 431, 979, 1004, 1009, 1034
Bse118I	RCCGGY	2 cut(s)	338, 1309
Bse1I	ACTGG	3 cut(s)	1112, 1215, 1510
Bse3DI	GCAATG	1 cut(s)	384
BseBI	CCWGG	2 cut(s)	1281, 1624
BseDI	CCNNGG	5 cut(s)	574, 606, 607, 730, 1280
BseGI	GGATG	4 cut(s)	209, 1254, 1469, 1682
BseMI	GCAATG	1 cut(s)	384
BseMII	CTCAG	4 cut(s)	167, 197, 1362, 1484
BseNI	ACTGG	3 cut(s)	1112, 1215, 1510
BsePI	GCGCGC	2 cut(s)	505, 550
BseRI	GAGGAG	6 cut(s)	793, 1025, 1156, 1208, 1551, 1690
BseXI	GCAGC	5 cut(s)	603, 1081, 1185, 1188, 1518
BseYI	CCCAGC	1 cut(s)	668
BsgI	GTGCAG	1 cut(s)	1136
Bsh1236I	CGCG	7 cut(s)	228, 492, 507, 552, 633, 656, 1526
Bsh1285I	CGRYCG	2 cut(s)	223, 640
BshFI	GGCC	7 cut(s)	5, 50, 524, 1069, 1153, 1238, 1428
BshNI	GGYRCC	2 cut(s)	311, 1108
BshTI	ACCGGT	1 cut(s)	1309
BsiEI	CGRYCG	2 cut(s)	223, 640
BsiHKAI	GWGCWC	5 cut(s)	219, 380, 924, 1362, 1561
BsiHKCI	CYCGRG	5 cut(s)	607, 730, 819, 859, 917
BslFI	GGGAC	3 cut(s)	307, 1297, 1301
BsmAI	GTCTC	1 cut(s)	1650
BsmFI	GGGAC	3 cut(s)	307, 1297, 1301
BsnI	GGCC	7 cut(s)	5, 50, 524, 1069, 1153, 1238, 1428
BsoBI	CYCGRG	5 cut(s)	607, 730, 819, 859, 917
Bsp1286I	GDGCHC	5 cut(s)	219, 380, 924, 1362, 1561
BspANI	GGCC	7 cut(s)	5, 50, 524, 1069, 1153, 1238, 1428
BspCNI	CTCAG	4 cut(s)	166, 196, 1363, 1485
BspFNI	CGCG	7 cut(s)	228, 492, 507, 552, 633, 656, 1526
BspHI	TCATGA	1 cut(s)	1414
BspLI	GGNNCC	4 cut(s)	313, 407, 1110, 1317
BspMAI	CTGCAG	1 cut(s)	357
BspMI	ACCTGC	1 cut(s)	895
BspPI	GGATC	5 cut(s)	140, 151, 212, 538, 619
BspT107I	GGYRCC	2 cut(s)	311, 1108
BsrBI	CCGCTC	3 cut(s)	43, 417, 858
BsrDI	GCAATG	1 cut(s)	384
BsrFI	RCCGGY	2 cut(s)	338, 1309
BsrI	ACTGG	3 cut(s)	1112, 1215, 1510
BssAI	RCCGGY	2 cut(s)	338, 1309
BssECI	CCNNGG	5 cut(s)	574, 606, 607, 730, 1280
BssHII	GCGCGC	2 cut(s)	505, 550
BssNI	GRCGYC	4 cut(s)	26, 312, 741, 1109
BssSI	CACGAG	1 cut(s)	1672
Bst2BI	CACGAG	1 cut(s)	1672
Bst2UI	CCWGG	2 cut(s)	1281, 1624
Bst4CI	ACNGT	4 cut(s)	995, 1324, 1407, 1556
Bst6I	CTCTTC	3 cut(s)	575, 1431, 1594
BstACI	GRCGYC	4 cut(s)	26, 312, 741, 1109
BstBAI	YACGTR	1 cut(s)	474
BstC8I	GCNNGC	9 cut(s)	310, 340, 507, 522, 552, 902, 1430, 1528, 1532
BstDEI	CTNAG	4 cut(s)	153, 183, 1371, 1493
BstENI	CCTNNNNNAGG	1 cut(s)	182
BstF5I	GGATG	4 cut(s)	209, 1254, 1469, 1682
BstFNI	CGCG	7 cut(s)	228, 492, 507, 552, 633, 656, 1526
BstH2I	RGCGCY	2 cut(s)	315, 1112
BstHHI	GCGC	8 cut(s)	314, 492, 507, 509, 552, 554, 1111, 1459
BstMAI	GTCTC	1 cut(s)	1650
BstMCI	CGRYCG	2 cut(s)	223, 640
BstNI	CCWGG	2 cut(s)	1281, 1624
BstSCI	CCNGG	8 cut(s)	69, 606, 607, 729, 730, 1064, 1279, 1622
BstSFI	CTRYAG	1 cut(s)	353
BstUI	CGCG	7 cut(s)	228, 492, 507, 552, 633, 656, 1526
BstV1I	GCAGC	5 cut(s)	603, 1081, 1185, 1188, 1518
BstV2I	GAAGAC	1 cut(s)	1512
BstX2I	RGATCY	4 cut(s)	156, 204, 480, 543
BstYI	RGATCY	4 cut(s)	156, 204, 480, 543
BsuRI	GGCC	7 cut(s)	5, 50, 524, 1069, 1153, 1238, 1428
BtgZI	GCGATG	1 cut(s)	679
BtrI	CACGTC	2 cut(s)	319, 516
BtsCI	GGATG	4 cut(s)	209, 1254, 1469, 1682
BtsI	GCAGTG	1 cut(s)	1575
BtsIMutI	CAGTG	3 cut(s)	1119, 1329, 1575
BveI	ACCTGC	1 cut(s)	895
Cac8I	GCNNGC	9 cut(s)	310, 340, 507, 522, 552, 902, 1430, 1528, 1532
CciI	TCATGA	1 cut(s)	1414
CfoI	GCGC	8 cut(s)	314, 492, 507, 509, 552, 554, 1111, 1459
Cfr10I	RCCGGY	2 cut(s)	338, 1309
Cfr13I	GGNCC	5 cut(s)	192, 768, 1067, 1083, 1315
Cfr9I	CCCGGG	2 cut(s)	607, 730
CpoI	CGGWCCG	1 cut(s)	192
CseI	GACGC	6 cut(s)	34, 273, 662, 790, 890, 1033
Csp6I	GTAC	5 cut(s)	74, 475, 724, 826, 1450
CspAI	ACCGGT	1 cut(s)	1309
CspCI	CAANNNNNGTGG	2 cut(s)	1293, 1328
CspI	CGGWCCG	1 cut(s)	192
CviAII	CATG	3 cut(s)	679, 736, 1415
CviQI	GTAC	5 cut(s)	74, 475, 724, 826, 1450
DdeI	CTNAG	4 cut(s)	153, 183, 1371, 1493
DinI	GGCGCC	2 cut(s)	313, 1110
DriI	GACNNNNNGTC	2 cut(s)	283, 1054
EaeI	YGGCCR	2 cut(s)	3, 522
Eam1104I	CTCTTC	3 cut(s)	575, 1431, 1594
Eam1105I	GACNNNNNGTC	2 cut(s)	283, 1054
EarI	CTCTTC	3 cut(s)	575, 1431, 1594
EciI	GGCGGA	6 cut(s)	27, 277, 325, 781, 968, 1513
Ecl136II	GAGCTC	3 cut(s)	378, 922, 1360
Eco24I	GRGCYC	3 cut(s)	380, 924, 1362
Eco47I	GGWCC	4 cut(s)	192, 768, 1083, 1315
Eco53kI	GAGCTC	3 cut(s)	378, 922, 1360
Eco88I	CYCGRG	5 cut(s)	607, 730, 819, 859, 917
EcoICRI	GAGCTC	3 cut(s)	378, 922, 1360
EcoNI	CCTNNNNNAGG	1 cut(s)	182
EcoRI	GAATTC	1 cut(s)	972
EcoRII	CCWGG	2 cut(s)	1279, 1622
EcoT38I	GRGCYC	3 cut(s)	380, 924, 1362
EgeI	GGCGCC	2 cut(s)	313, 1110
EheI	GGCGCC	2 cut(s)	313, 1110
FaeI	CATG	3 cut(s)	682, 739, 1418
FaiI	YATR	6 cut(s)	81, 367, 680, 737, 1416, 1620
FaqI	GGGAC	3 cut(s)	307, 1297, 1301
FatI	CATG	3 cut(s)	678, 735, 1414
FauI	CCCGC	2 cut(s)	1284, 1371
FblI	GTMKAC	1 cut(s)	1047
FokI	GGATG	4 cut(s)	196, 1261, 1456, 1669
FriOI	GRGCYC	3 cut(s)	380, 924, 1362
FspBI	CTAG	3 cut(s)	299, 758, 1611
GlaI	GCGC	8 cut(s)	313, 491, 506, 508, 551, 553, 1110, 1458
GsaI	CCCAGC	1 cut(s)	672
GsuI	CTGGAG	1 cut(s)	311
HaeII	RGCGCY	2 cut(s)	315, 1112
HaeIII	GGCC	7 cut(s)	5, 50, 524, 1069, 1153, 1238, 1428
HgaI	GACGC	6 cut(s)	34, 273, 662, 790, 890, 1033
HhaI	GCGC	8 cut(s)	314, 492, 507, 509, 552, 554, 1111, 1459
Hin1I	GRCGYC	4 cut(s)	26, 312, 741, 1109
Hin1II	CATG	3 cut(s)	682, 739, 1418
Hin6I	GCGC	8 cut(s)	312, 490, 505, 507, 550, 552, 1109, 1457
HinP1I	GCGC	8 cut(s)	312, 490, 505, 507, 550, 552, 1109, 1457
HincII	GTYRAC	1 cut(s)	1048
HindII	GTYRAC	1 cut(s)	1048
HinfI	GANTC	9 cut(s)	117, 151, 167, 173, 346, 686, 702, 958, 1586
HphI	GGTGA	3 cut(s)	1243, 1255, 1345
Hpy166II	GTNNAC	6 cut(s)	76, 724, 826, 1048, 1344, 1671
Hpy8I	GTNNAC	6 cut(s)	76, 724, 826, 1048, 1344, 1671
HpyAV	CCTTC	6 cut(s)	140, 247, 407, 454, 1228, 1412
HpyCH4III	ACNGT	4 cut(s)	995, 1324, 1407, 1556
HpyCH4IV	ACGT	9 cut(s)	231, 279, 318, 473, 515, 600, 741, 1050, 1340
HpyCH4V	TGCA	2 cut(s)	355, 1117
HpyF3I	CTNAG	4 cut(s)	153, 183, 1371, 1493
HpySE526I	ACGT	9 cut(s)	231, 279, 318, 473, 515, 600, 741, 1050, 1340
Hsp92I	GRCGYC	4 cut(s)	26, 312, 741, 1109
Hsp92II	CATG	3 cut(s)	682, 739, 1418
HspAI	GCGC	8 cut(s)	312, 490, 505, 507, 550, 552, 1109, 1457
KasI	GGCGCC	2 cut(s)	311, 1108
KroI	GCCGGC	1 cut(s)	338
KroNI	GCCGGC	1 cut(s)	340
Lsp1109I	GCAGC	5 cut(s)	603, 1081, 1185, 1188, 1518
LweI	GCATC	1 cut(s)	1641
MaeI	CTAG	3 cut(s)	299, 758, 1611
MaeII	ACGT	9 cut(s)	231, 279, 318, 473, 515, 600, 741, 1050, 1340
MaeIII	GTNAC	3 cut(s)	742, 1206, 1570
MbiI	CCGCTC	3 cut(s)	43, 417, 858
MboII	GAAGA	9 cut(s)	466, 553, 562, 640, 1372, 1448, 1512, 1581, 1669
MfeI	CAATTG	1 cut(s)	772
MflI	RGATCY	4 cut(s)	156, 204, 480, 543
MhlI	GDGCHC	5 cut(s)	219, 380, 924, 1362, 1561
MlsI	TGGCCA	1 cut(s)	5
MluCI	AATT	6 cut(s)	294, 772, 831, 972, 1059, 1376
MluI	ACGCGT	1 cut(s)	654
MluNI	TGGCCA	1 cut(s)	5
Mly113I	GGCGCC	2 cut(s)	312, 1109
MlyI	GAGTC	5 cut(s)	145, 167, 176, 695, 1580
MmeI	TCCRAC	6 cut(s)	173, 195, 405, 492, 669, 784
Mox20I	TGGCCA	1 cut(s)	5
MroNI	GCCGGC	1 cut(s)	338
MroXI	GAANNNNTTC	2 cut(s)	647, 1133
MscI	TGGCCA	1 cut(s)	5
MseI	TTAA	3 cut(s)	392, 1184, 1296
MslI	CAYNNNNRTG	2 cut(s)	1388, 1486
Msp20I	TGGCCA	1 cut(s)	5
MspA1I	CMGCKG	2 cut(s)	197, 1094
MspR9I	CCNGG	8 cut(s)	71, 608, 609, 731, 732, 1066, 1281, 1624
MunI	CAATTG	1 cut(s)	772
MvaI	CCWGG	2 cut(s)	1281, 1624
MvnI	CGCG	7 cut(s)	228, 492, 507, 552, 633, 656, 1526
NaeI	GCCGGC	1 cut(s)	340
NarI	GGCGCC	2 cut(s)	312, 1109
NciI	CCSGG	6 cut(s)	71, 608, 609, 731, 732, 1066
NgoMIV	GCCGGC	1 cut(s)	338
NlaIII	CATG	3 cut(s)	682, 739, 1418
NlaIV	GGNNCC	4 cut(s)	313, 407, 1110, 1317
NmeAIII	GCCGAG	1 cut(s)	555
NmuCI	GTSAC	2 cut(s)	742, 1570
PaeR7I	CTCGAG	3 cut(s)	819, 859, 917
PagI	TCATGA	1 cut(s)	1414
PaqCI	CACCTGC	1 cut(s)	895
PauI	GCGCGC	2 cut(s)	505, 550
PdiI	GCCGGC	1 cut(s)	340
PdmI	GAANNNNTTC	2 cut(s)	647, 1133
PfeI	GAWTC	4 cut(s)	117, 346, 702, 958
PflFI	GACNNNGTC	1 cut(s)	28
PinAI	ACCGGT	1 cut(s)	1309
Ple19I	CGATCG	1 cut(s)	640
PleI	GAGTC	5 cut(s)	145, 167, 175, 694, 1580
PluTI	GGCGCC	2 cut(s)	315, 1112
PpsI	GAGTC	5 cut(s)	145, 167, 175, 694, 1580
Ppu21I	YACGTR	1 cut(s)	474
Psp124BI	GAGCTC	3 cut(s)	380, 924, 1362
Psp1406I	AACGTT	1 cut(s)	1340
Psp6I	CCWGG	2 cut(s)	1279, 1622
PspFI	CCCAGC	1 cut(s)	668
PspGI	CCWGG	2 cut(s)	1279, 1622
PspN4I	GGNNCC	4 cut(s)	313, 407, 1110, 1317
PspPI	GGNCC	5 cut(s)	192, 768, 1067, 1083, 1315
PspXI	VCTCGAGB	1 cut(s)	819
PstI	CTGCAG	1 cut(s)	357
PsuI	RGATCY	4 cut(s)	156, 204, 480, 543
PsyI	GACNNNGTC	1 cut(s)	28
PteI	GCGCGC	2 cut(s)	505, 550
PvuI	CGATCG	1 cut(s)	640
PvuII	CAGCTG	1 cut(s)	1094
RsaI	GTAC	5 cut(s)	75, 476, 725, 827, 1451
RsaNI	GTAC	5 cut(s)	74, 475, 724, 826, 1450
RseI	CAYNNNNRTG	2 cut(s)	1388, 1486
Rsr2I	CGGWCCG	1 cut(s)	192
RsrII	CGGWCCG	1 cut(s)	192
SacI	GAGCTC	3 cut(s)	380, 924, 1362
SalI	GTCGAC	1 cut(s)	1046
SaqAI	TTAA	3 cut(s)	392, 1184, 1296
Sau96I	GGNCC	5 cut(s)	192, 768, 1067, 1083, 1315
SbfI	CCTGCAGG	1 cut(s)	357
SchI	GAGTC	5 cut(s)	145, 167, 176, 695, 1580
ScrFI	CCNGG	8 cut(s)	71, 608, 609, 731, 732, 1066, 1281, 1624
SdaI	CCTGCAGG	1 cut(s)	357
SduI	GDGCHC	5 cut(s)	219, 380, 924, 1362, 1561
SfaNI	GCATC	1 cut(s)	1641
SfcI	CTRYAG	1 cut(s)	353
SfoI	GGCGCC	2 cut(s)	313, 1110
Sfr274I	CTCGAG	3 cut(s)	819, 859, 917
SgrDI	CGTCGACG	1 cut(s)	1046
SinI	GGWCC	4 cut(s)	192, 768, 1083, 1315
SlaI	CTCGAG	3 cut(s)	819, 859, 917
SmaI	CCCGGG	2 cut(s)	609, 732
SmiMI	CAYNNNNRTG	2 cut(s)	1388, 1486
SmlI	CTYRAG	6 cut(s)	44, 819, 859, 917, 1147, 1581
SmoI	CTYRAG	6 cut(s)	44, 819, 859, 917, 1147, 1581
SrfI	GCCCGGGC	1 cut(s)	732
Sse8387I	CCTGCAGG	1 cut(s)	357
Sse9I	AATT	6 cut(s)	294, 772, 831, 972, 1059, 1376
SspDI	GGCGCC	2 cut(s)	311, 1108
SspMI	CTAG	3 cut(s)	299, 758, 1611
SstI	GAGCTC	3 cut(s)	380, 924, 1362
StyD4I	CCNGG	8 cut(s)	69, 606, 607, 729, 730, 1064, 1279, 1622
TaaI	ACNGT	4 cut(s)	995, 1324, 1407, 1556
TaiI	ACGT	9 cut(s)	234, 282, 321, 476, 518, 603, 744, 1053, 1343
TasI	AATT	6 cut(s)	294, 772, 831, 972, 1059, 1376
TatI	WGTACW	1 cut(s)	1449
TauI	GCSGC	6 cut(s)	43, 228, 1072, 1106, 1109, 1580
TfiI	GAWTC	4 cut(s)	117, 346, 702, 958
Tru1I	TTAA	3 cut(s)	392, 1184, 1296
Tru9I	TTAA	3 cut(s)	392, 1184, 1296
TscAI	CASTG	3 cut(s)	1119, 1329, 1575
TseFI	GTSAC	2 cut(s)	742, 1570
TseI	GCWGC	5 cut(s)	616, 1094, 1198, 1201, 1506
Tsp45I	GTSAC	2 cut(s)	742, 1570
TspDTI	ATGAA	3 cut(s)	1403, 1431, 1670
TspGWI	ACGGA	7 cut(s)	202, 205, 424, 549, 809, 1184, 1205
TspMI	CCCGGG	2 cut(s)	607, 730
TspRI	CASTG	3 cut(s)	1119, 1329, 1575
Tth111I	GACNNNGTC	1 cut(s)	28
VpaK11BI	GGWCC	4 cut(s)	192, 768, 1083, 1315
XagI	CCTNNNNNAGG	1 cut(s)	182
XapI	RAATTY	3 cut(s)	294, 831, 972
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1327
XhoI	CTCGAG	3 cut(s)	819, 859, 917
XmaI	CCCGGG	2 cut(s)	607, 730
XmiI	GTMKAC	1 cut(s)	1047
XmnI	GAANNNNTTC	2 cut(s)	647, 1133
XspI	CTAG	3 cut(s)	299, 758, 1611
ZraI	GACGTC	1 cut(s)	742

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.