Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr5B

Orthogroup ID

OG0013140

Physical Location & Seq

Genomic Coordinates Forward (+)

1817025 .. 1822405

Gene Structure 5381 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh5BG025900.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1530 bp

ATGGCAAAGCCGTCGTCGTCGTCCTCCTCGACGGCGTCGTTTCCGCCTCTCAAGCCCGACGACTACTCCCACAGCCGCGTCCACTACGCCGTCGTTTTGGCAGACCACACCACTCTCTCCCGAATCGTCTCCACGCTCCCCCGCCTCGCCGATCCGACTCAGATCCACACCGAGTCCGACTCGCTCTCCCAGGAGCGTGTCGCTGATAAGATCTCCGCCGTGCTCGACCGCCGCGACGTCCCTCACAGAGAAACCCCTCTCCATCTTGCTGTCCGGTTAAACGACGCCGTTTCGGCCAAAATCCTCGCCTCCGCCGGCGCCGACGTGTCGCTGCAGAACGCCGCCGGGTGGAACCCCTTGCAGGAGGCTCTCTGCCGCCGGAGCTCCGAGATCGCCTTGATCTTGCTCAAGCTCCACCACCGCTCCGCCTGGGCCAAGTGGCGCCGCCGCCTGCCACGTGTCATCGCTGTGCTGCGGAGAATGCGGGACTTCTACATGGAGATCTCGTTCCACTTCGAGAGCTCCGTCATTCCGTTCGTCGGGAAAATCGCTCCGTCCGACACGTACAAGATCTGGAAGCGAGACGGAAACCTACGCGCCGACACTTCGTTGGCCGGGTTCGACGGCCTGAAGATCCAGCGCGCCGATCAGAGCTTCCTCTTCCTCGGCGACGGCGACCAGACTCACGACGTTCCGCCGGGTTCACTGCTCGTGCTCAACCGCGACGACCGGAAAATCTTCGACGCCTTTGAGAACGCCGGGTCGCCGATGACCGAGTCCGACATCGCCGGATTCTGCTCCCAGACGAGCGTCTACCGCCCCGGCATGGACGTCACCAGAGCCGAGCTCGTCGGCCGGACCAATTGGCGTCGGCAGGAGAAGACCGAATCCGTCGGAGAGTGGAAGGCCAGAGTCTTCGAAATGCACAACGTCGTCTTCAGCTTCCGGTCCCGGAAAGTCGCCAATCCGGATAATGCTGACGTGGCGGCGGGAAGCGAGCAGGTCCTCCCTCTAGAGCTAGACGAAGACGACGACGGTTTTTTAGTGGCGGAGAATCCGAGCATGTTCGGAATCCCCGACGGCCGGAGGCACAGTAGCTTCATCAGAGAGGACCGGGAGTTCGTGGCATTGGGGAGAAAAAGCGTCGACGTGACGTTTCAGCCGCCGCGGAGGTCCATGGCGGCGACCGTTACGGCGGCGCCGGTCCAGACCAAAGAGAAAGAGTACGTCAAGAGCCTGAGACCCTCCGTTTGGCTGACGGACCATTTTCCGTTAAAGACGGAGGAGCTGCTGCCGTTACTGGATATTCTGGCGAGTAAAGTGAAGGCGGTTAGGAGAATGAGGGAGCTGCTCACTACCAAGTTCCCGCCAGGGACGTTTCCGGTGAAGGTAAGGGCATTTATGACATTTCATTATTCAAAACCGCCTCTCGGGCCCCACTTAAGATTTGGGTCATGGGTCTCGGGTCGTCGTCGTCGTCTTTATATGTCTCTCTGGTTTGCTTTTTGTTGCTTTCCATGCTTAAGCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005102 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0006621 GO:0008104 GO:0008150 GO:0009889 GO:0009987 GO:0010556 GO:0010869 GO:0010941 GO:0012505 GO:0019222 GO:0031323 GO:0032507 GO:0033036 GO:0033365 GO:0034613 GO:0035437 GO:0042981 GO:0043067 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0045185 GO:0048471 GO:0050789 GO:0050794 GO:0051179 GO:0051235 GO:0051641 GO:0051651 GO:0060255 GO:0065007 GO:0065008 GO:0070727 GO:0070972 GO:0072595 GO:2000209

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Ank_2 Ank_4 GPCR_chapero_1

Protein Analysis

509

Amino Acids

57.46

Weight (kDa)

9.46

Isoelectric Point (pI)

55.33

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Ank_2	PF12796	82 - 137	7.5e-06	Ankyrin repeats (3 copies)
ANKRD13_C	PF11904	199 - 465	3.4e-82	ANKRD13 C-terminal

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0013140)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G62050
fragaria_vesca	FvH4_3g01950
malus_domestica	MD05G1347900.v1.1 MD10G1322300.v1.1
prunus_persica	Prupe.4G019600_v2.0.a1
pyrus_communis	pycom05g31540 pycom10g27280
rosa_chinensis	RchiOBHm_Chr5g0002861
rosa_laevigata	RLG00000031066
rosa_multiflora	Rmu_sc0013603.1_g000009 Rmu_sc0020722.1_g000002
rosa_roxburghii	Rroxscaffold_1G00072990
rosa_rugosa	Rorug04G0400300
rosa_samantha	Rh5AG023200 Rh5BG025900 Rh5CG025800 Rh5DG024300
rosa_wichuraiana	Rw5G002160

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	2 cut(s)	240, 834
Acc36I	ACCTGC	1 cut(s)	991
AccB1I	GGYRCC	3 cut(s)	317, 441, 1198
AccBSI	CCGCTC	1 cut(s)	423
AccI	GTMKAC	2 cut(s)	813, 1146
AccII	CGCG	6 cut(s)	78, 234, 597, 642, 723, 1168
AccIII	TCCGGA	1 cut(s)	967
AclWI	GGATC	3 cut(s)	146, 157, 628
AcoI	YGGCCR	4 cut(s)	294, 612, 853, 1081
AcuI	CTGAAG	2 cut(s)	650, 922
AcvI	CACGTG	1 cut(s)	458
AcyI	GRCGYC	9 cut(s)	35, 237, 285, 318, 442, 744, 831, 868, 1199
AfaI	GTAC	2 cut(s)	566, 1226
AfiI	CCNNNNNNNGG	7 cut(s)	348, 361, 539, 826, 1083, 1251, 1430
AflII	CTTAAG	2 cut(s)	1441, 1522
AflIII	ACRYGT	3 cut(s)	324, 457, 561
AgsI	TTSAA	1 cut(s)	1419
AjiI	CACGTC	3 cut(s)	325, 982, 1150
AjnI	CCWGG	3 cut(s)	189, 428, 1369
AloI	GAACNNNNNNTCC	4 cut(s)	491, 523, 1103, 1135
Alw21I	GWGCWC	5 cut(s)	225, 386, 524, 717, 849
Alw26I	GTCTC	5 cut(s)	133, 576, 1234, 1465, 1494
AlwI	GGATC	3 cut(s)	146, 157, 628
Ama87I	CYCGRG	2 cut(s)	1430, 1462
Aor13HI	TCCGGA	1 cut(s)	967
AoxI	GGCC	8 cut(s)	294, 432, 612, 625, 853, 906, 1081, 1433
ApaI	GGGCCC	1 cut(s)	1437
ApeKI	GCWGC	5 cut(s)	331, 472, 1288, 1291, 1348
ArsI	GACNNNNNNTTYG	5 cut(s)	31, 1103, 1135, 1233, 1265
Asp700I	GAANNNNTTC	1 cut(s)	737
AspLEI	GCGC	6 cut(s)	320, 444, 599, 642, 644, 1201
AsuC2I	CCSGG	7 cut(s)	346, 616, 699, 760, 822, 952, 1115
AsuHPI	GGTGA	2 cut(s)	826, 1396
AsuII	TTCGAA	1 cut(s)	918
AvaI	CYCGRG	2 cut(s)	1430, 1462
AvaII	GGWCC	7 cut(s)	858, 948, 1003, 1111, 1173, 1204, 1261
BaeGI	GKGCMC	1 cut(s)	1437
BanI	GGYRCC	3 cut(s)	317, 441, 1198
BanII	GRGCYC	4 cut(s)	386, 524, 849, 1437
BauI	CACGAG	1 cut(s)	710
BbrPI	CACGTG	1 cut(s)	458
BbsI	GAAGAC	4 cut(s)	887, 907, 928, 1032
Bbv12I	GWGCWC	5 cut(s)	225, 386, 524, 717, 849
BbvI	GCAGC	5 cut(s)	318, 459, 1275, 1278, 1335
BccI	CCATC	1 cut(s)	270
BceAI	ACGGC	9 cut(s)	48, 74, 203, 272, 640, 688, 1096, 1209, 1279
BcgI	CGANNNNNNTGC	1 cut(s)	28
BciT130I	CCWGG	3 cut(s)	191, 430, 1371
BcnI	CCSGG	7 cut(s)	346, 616, 699, 760, 822, 952, 1115
BcoDI	GTCTC	5 cut(s)	133, 576, 1234, 1465, 1494
BfaI	CTAG	2 cut(s)	1013, 1019
BfmI	CTRYAG	1 cut(s)	332
BfoI	RGCGCY	3 cut(s)	321, 445, 1202
BfrI	CTTAAG	2 cut(s)	1441, 1522
BfuAI	ACCTGC	1 cut(s)	991
BglI	GCCNNNNNGGC	2 cut(s)	293, 1432
BglII	AGATCT	3 cut(s)	210, 501, 570
Bme18I	GGWCC	7 cut(s)	858, 948, 1003, 1111, 1173, 1204, 1261
BmeT110I	CYCGRG	2 cut(s)	1430, 1462
BmgBI	CACGTC	3 cut(s)	325, 982, 1150
BmiI	GGNNCC	7 cut(s)	319, 353, 443, 950, 1200, 1435, 1436
BoxI	GACNNNNGTC	1 cut(s)	809
BpiI	GAAGAC	4 cut(s)	887, 907, 928, 1032
BplI	GAGNNNNNCTC	4 cut(s)	164, 196, 831, 863
Bpu14I	TTCGAA	1 cut(s)	918
BpuEI	CTTGAG	2 cut(s)	35, 392
BpuMI	CCSGG	7 cut(s)	346, 616, 699, 760, 822, 952, 1115
BsaAI	YACGTR	2 cut(s)	458, 564
BsaHI	GRCGYC	9 cut(s)	35, 237, 285, 318, 442, 744, 831, 868, 1199
BsaI	GGTCTC	2 cut(s)	1234, 1465
BsaJI	CCNNGG	7 cut(s)	189, 429, 664, 820, 1166, 1176, 1370
BsaWI	WCCGGW	5 cut(s)	273, 729, 945, 967, 1381
Bsc4I	CCNNNNNNNGG	7 cut(s)	348, 361, 539, 826, 1083, 1251, 1430
Bse118I	RCCGGY	2 cut(s)	314, 1201
Bse1I	ACTGG	1 cut(s)	1305
BseAI	TCCGGA	1 cut(s)	967
BseBI	CCWGG	3 cut(s)	191, 430, 1371
BseDI	CCNNGG	7 cut(s)	189, 429, 664, 820, 1166, 1176, 1370
BseLI	CCNNNNNNNGG	7 cut(s)	348, 361, 539, 826, 1083, 1251, 1430
BseMII	CTCAG	2 cut(s)	173, 1229
BseNI	ACTGG	1 cut(s)	1305
BsePI	GCGCGC	1 cut(s)	640
BseRI	GAGGAG	2 cut(s)	16, 1298
BseSI	GKGCMC	1 cut(s)	1437
BseX3I	CGGCCG	2 cut(s)	853, 1081
BseXI	GCAGC	5 cut(s)	318, 459, 1275, 1278, 1335
Bsh1236I	CGCG	6 cut(s)	78, 234, 597, 642, 723, 1168
Bsh1285I	CGRYCG	5 cut(s)	229, 730, 856, 1084, 1188
BshFI	GGCC	8 cut(s)	296, 434, 614, 627, 855, 908, 1083, 1435
BshNI	GGYRCC	3 cut(s)	317, 441, 1198
BsiEI	CGRYCG	5 cut(s)	229, 730, 856, 1084, 1188
BsiHKAI	GWGCWC	5 cut(s)	225, 386, 524, 717, 849
BsiHKCI	CYCGRG	2 cut(s)	1430, 1462
BslFI	GGGAC	4 cut(s)	224, 500, 934, 1387
BslI	CCNNNNNNNGG	7 cut(s)	348, 361, 539, 826, 1083, 1251, 1430
BsmAI	GTCTC	5 cut(s)	133, 576, 1234, 1465, 1494
BsmBI	CGTCTC	2 cut(s)	133, 576
BsmFI	GGGAC	4 cut(s)	224, 500, 934, 1387
BsmI	GAATGC	1 cut(s)	486
BsnI	GGCC	8 cut(s)	296, 434, 614, 627, 855, 908, 1083, 1435
Bso31I	GGTCTC	2 cut(s)	1234, 1465
BsoBI	CYCGRG	2 cut(s)	1430, 1462
Bsp119I	TTCGAA	1 cut(s)	918
Bsp120I	GGGCCC	1 cut(s)	1433
Bsp1286I	GDGCHC	6 cut(s)	225, 386, 524, 717, 849, 1437
Bsp13I	TCCGGA	1 cut(s)	967
Bsp143I	GATC	9 cut(s)	151, 162, 210, 390, 399, 501, 570, 633, 646
Bsp19I	CCATGG	1 cut(s)	1176
BspANI	GGCC	8 cut(s)	296, 434, 614, 627, 855, 908, 1083, 1435
BspCNI	CTCAG	2 cut(s)	172, 1230
BspEI	TCCGGA	1 cut(s)	967
BspFNI	CGCG	6 cut(s)	78, 234, 597, 642, 723, 1168
BspLI	GGNNCC	7 cut(s)	319, 353, 443, 950, 1200, 1435, 1436
BspMAI	CTGCAG	1 cut(s)	336
BspMI	ACCTGC	1 cut(s)	991
BspPI	GGATC	3 cut(s)	146, 157, 628
BspT104I	TTCGAA	1 cut(s)	918
BspT107I	GGYRCC	3 cut(s)	317, 441, 1198
BspTI	CTTAAG	2 cut(s)	1441, 1522
BspTNI	GGTCTC	2 cut(s)	1234, 1465
BsrBI	CCGCTC	1 cut(s)	423
BsrFI	RCCGGY	2 cut(s)	314, 1201
BsrI	ACTGG	1 cut(s)	1305
BssAI	RCCGGY	2 cut(s)	314, 1201
BssECI	CCNNGG	7 cut(s)	189, 429, 664, 820, 1166, 1176, 1370
BssHII	GCGCGC	1 cut(s)	640
BssMI	GATC	9 cut(s)	151, 162, 210, 390, 399, 501, 570, 633, 646
BssNI	GRCGYC	9 cut(s)	35, 237, 285, 318, 442, 744, 831, 868, 1199
BssSI	CACGAG	1 cut(s)	710
BssT1I	CCWWGG	1 cut(s)	1176
Bst2BI	CACGAG	1 cut(s)	710
Bst2UI	CCWGG	3 cut(s)	191, 430, 1371
Bst4CI	ACNGT	3 cut(s)	1037, 1094, 1189
Bst6I	CTCTTC	1 cut(s)	665
BstACI	GRCGYC	9 cut(s)	35, 237, 285, 318, 442, 744, 831, 868, 1199
BstAFI	CTTAAG	2 cut(s)	1441, 1522
BstBAI	YACGTR	2 cut(s)	458, 564
BstBI	TTCGAA	1 cut(s)	918
BstC8I	GCNNGC	4 cut(s)	316, 452, 642, 998
BstDEI	CTNAG	2 cut(s)	159, 1238
BstDSI	CCRYGG	2 cut(s)	1166, 1176
BstFNI	CGCG	6 cut(s)	78, 234, 597, 642, 723, 1168
BstH2I	RGCGCY	3 cut(s)	321, 445, 1202
BstHHI	GCGC	6 cut(s)	320, 444, 599, 642, 644, 1201
BstKTI	GATC	9 cut(s)	154, 165, 213, 393, 402, 504, 573, 636, 649
BstMAI	GTCTC	5 cut(s)	133, 576, 1234, 1465, 1494
BstMBI	GATC	9 cut(s)	151, 162, 210, 390, 399, 501, 570, 633, 646
BstMCI	CGRYCG	5 cut(s)	229, 730, 856, 1084, 1188
BstMWI	GCNNNNNNNGC	8 cut(s)	52, 293, 381, 481, 816, 983, 1432, 1518
BstNI	CCWGG	3 cut(s)	191, 430, 1371
BstNSI	RCATGY	1 cut(s)	1066
BstPAI	GACNNNNGTC	1 cut(s)	809
BstSFI	CTRYAG	1 cut(s)	332
BstSLI	GKGCMC	1 cut(s)	1437
BstUI	CGCG	6 cut(s)	78, 234, 597, 642, 723, 1168
BstV1I	GCAGC	5 cut(s)	318, 459, 1275, 1278, 1335
BstV2I	GAAGAC	4 cut(s)	887, 907, 928, 1032
BstX2I	RGATCY	5 cut(s)	162, 210, 501, 570, 633
BstYI	RGATCY	5 cut(s)	162, 210, 501, 570, 633
BstZI	CGGCCG	2 cut(s)	853, 1081
BsuRI	GGCC	8 cut(s)	296, 434, 614, 627, 855, 908, 1083, 1435
BtgI	CCRYGG	2 cut(s)	1166, 1176
BtgZI	GCGATG	2 cut(s)	448, 769
BtrI	CACGTC	3 cut(s)	325, 982, 1150
BtsI	GCAGTG	1 cut(s)	704
BtsIMutI	CAGTG	1 cut(s)	704
BveI	ACCTGC	1 cut(s)	991
Cac8I	GCNNGC	4 cut(s)	316, 452, 642, 998
CfoI	GCGC	6 cut(s)	320, 444, 599, 642, 644, 1201
Cfr10I	RCCGGY	2 cut(s)	314, 1201
Cfr42I	CCGCGG	1 cut(s)	1169
CseI	GACGC	7 cut(s)	24, 67, 293, 752, 799, 857, 1132
Csp6I	GTAC	2 cut(s)	565, 1225
CviAII	CATG	6 cut(s)	496, 826, 1063, 1177, 1455, 1518
CviQI	GTAC	2 cut(s)	565, 1225
DdeI	CTNAG	2 cut(s)	159, 1238
DinI	GGCGCC	3 cut(s)	319, 443, 1200
DpnI	GATC	9 cut(s)	153, 164, 212, 392, 401, 503, 572, 635, 648
DpnII	GATC	9 cut(s)	151, 162, 210, 390, 399, 501, 570, 633, 646
EaeI	YGGCCR	4 cut(s)	294, 612, 853, 1081
EagI	CGGCCG	2 cut(s)	853, 1081
Eam1104I	CTCTTC	1 cut(s)	665
EarI	CTCTTC	1 cut(s)	665
EciI	GGCGGA	6 cut(s)	33, 205, 301, 415, 684, 1064
Ecl136II	GAGCTC	3 cut(s)	384, 522, 847
EclXI	CGGCCG	2 cut(s)	853, 1081
Eco130I	CCWWGG	1 cut(s)	1176
Eco24I	GRGCYC	4 cut(s)	386, 524, 849, 1437
Eco31I	GGTCTC	2 cut(s)	1234, 1465
Eco47I	GGWCC	7 cut(s)	858, 948, 1003, 1111, 1173, 1204, 1261
Eco52I	CGGCCG	2 cut(s)	853, 1081
Eco53kI	GAGCTC	3 cut(s)	384, 522, 847
Eco57I	CTGAAG	2 cut(s)	650, 922
Eco72I	CACGTG	1 cut(s)	458
Eco88I	CYCGRG	2 cut(s)	1430, 1462
EcoICRI	GAGCTC	3 cut(s)	384, 522, 847
EcoO109I	RGGNCCY	2 cut(s)	1003, 1434
EcoRII	CCWGG	3 cut(s)	189, 428, 1369
EcoT14I	CCWWGG	1 cut(s)	1176
EcoT38I	GRGCYC	4 cut(s)	386, 524, 849, 1437
EgeI	GGCGCC	3 cut(s)	319, 443, 1200
EheI	GGCGCC	3 cut(s)	319, 443, 1200
ErhI	CCWWGG	1 cut(s)	1176
Esp3I	CGTCTC	2 cut(s)	133, 576
FaeI	CATG	6 cut(s)	499, 829, 1066, 1180, 1458, 1521
FaiI	YATR	9 cut(s)	497, 827, 1064, 1178, 1403, 1456, 1485, 1487, 1519
FaqI	GGGAC	4 cut(s)	224, 500, 934, 1387
FatI	CATG	6 cut(s)	495, 825, 1062, 1176, 1454, 1517
FauI	CCCGC	4 cut(s)	149, 477, 982, 1374
FblI	GTMKAC	2 cut(s)	813, 1146
FriOI	GRGCYC	4 cut(s)	386, 524, 849, 1437
FspBI	CTAG	2 cut(s)	1013, 1019
GlaI	GCGC	6 cut(s)	319, 443, 598, 641, 643, 1200
HaeII	RGCGCY	3 cut(s)	321, 445, 1202
HaeIII	GGCC	8 cut(s)	296, 434, 614, 627, 855, 908, 1083, 1435
HgaI	GACGC	7 cut(s)	24, 67, 293, 752, 799, 857, 1132
HhaI	GCGC	6 cut(s)	320, 444, 599, 642, 644, 1201
Hin1I	GRCGYC	9 cut(s)	35, 237, 285, 318, 442, 744, 831, 868, 1199
Hin1II	CATG	6 cut(s)	499, 829, 1066, 1180, 1458, 1521
Hin6I	GCGC	6 cut(s)	318, 442, 597, 640, 642, 1199
HinP1I	GCGC	6 cut(s)	318, 442, 597, 640, 642, 1199
HincII	GTYRAC	1 cut(s)	1147
HindII	GTYRAC	1 cut(s)	1147
HphI	GGTGA	2 cut(s)	826, 1396
Hpy166II	GTNNAC	4 cut(s)	82, 704, 814, 1147
Hpy188III	TCNNGA	9 cut(s)	120, 517, 541, 574, 686, 968, 1013, 1207, 1231
Hpy8I	GTNNAC	4 cut(s)	82, 704, 814, 1147
HpyAV	CCTTC	3 cut(s)	898, 1318, 1381
HpyCH4III	ACNGT	3 cut(s)	1037, 1094, 1189
HpyCH4V	TGCA	3 cut(s)	334, 361, 925
HpyF10VI	GCNNNNNNNGC	8 cut(s)	52, 293, 381, 481, 816, 983, 1432, 1518
HpyF3I	CTNAG	2 cut(s)	159, 1238
Hsp92I	GRCGYC	9 cut(s)	35, 237, 285, 318, 442, 744, 831, 868, 1199
Hsp92II	CATG	6 cut(s)	499, 829, 1066, 1180, 1458, 1521
HspAI	GCGC	6 cut(s)	318, 442, 597, 640, 642, 1199
KasI	GGCGCC	3 cut(s)	317, 441, 1198
Kpn2I	TCCGGA	1 cut(s)	967
KroI	GCCGGC	1 cut(s)	314
KroNI	GCCGGC	1 cut(s)	316
KspI	CCGCGG	1 cut(s)	1169
Kzo9I	GATC	9 cut(s)	151, 162, 210, 390, 399, 501, 570, 633, 646
Lsp1109I	GCAGC	5 cut(s)	318, 459, 1275, 1278, 1335
MaeI	CTAG	2 cut(s)	1013, 1019
MaeIII	GTNAC	4 cut(s)	832, 1150, 1189, 1296
MalI	GATC	9 cut(s)	153, 164, 212, 392, 401, 503, 572, 635, 648
MbiI	CCGCTC	1 cut(s)	423
MboI	GATC	9 cut(s)	151, 162, 210, 390, 399, 501, 570, 633, 646
MboII	GAAGA	7 cut(s)	643, 652, 730, 892, 907, 928, 1037
MfeI	CAATTG	1 cut(s)	862
MflI	RGATCY	5 cut(s)	162, 210, 501, 570, 633
MhlI	GDGCHC	6 cut(s)	225, 386, 524, 717, 849, 1437
MluCI	AATT	1 cut(s)	862
Mly113I	GGCGCC	3 cut(s)	318, 442, 1199
MlyI	GAGTC	6 cut(s)	151, 173, 182, 676, 785, 921
MmeI	TCCRAC	5 cut(s)	179, 201, 582, 804, 874
MreI	CGCCGGCG	1 cut(s)	314
MroI	TCCGGA	1 cut(s)	967
MroNI	GCCGGC	1 cut(s)	314
MroXI	GAANNNNTTC	1 cut(s)	737
MseI	TTAA	4 cut(s)	278, 1274, 1442, 1523
MslI	CAYNNNNRTG	1 cut(s)	467
MspA1I	CMGCKG	1 cut(s)	1168
MspCI	CTTAAG	2 cut(s)	1441, 1522
MunI	CAATTG	1 cut(s)	862
Mva1269I	GAATGC	1 cut(s)	486
MvaI	CCWGG	3 cut(s)	191, 430, 1371
MvnI	CGCG	6 cut(s)	78, 234, 597, 642, 723, 1168
MwoI	GCNNNNNNNGC	8 cut(s)	52, 293, 381, 481, 816, 983, 1432, 1518
NaeI	GCCGGC	1 cut(s)	316
NarI	GGCGCC	3 cut(s)	318, 442, 1199
NciI	CCSGG	7 cut(s)	346, 616, 699, 760, 822, 952, 1115
NcoI	CCATGG	1 cut(s)	1176
NdeII	GATC	9 cut(s)	151, 162, 210, 390, 399, 501, 570, 633, 646
NgoMIV	GCCGGC	1 cut(s)	314
NlaIII	CATG	6 cut(s)	499, 829, 1066, 1180, 1458, 1521
NlaIV	GGNNCC	7 cut(s)	319, 353, 443, 950, 1200, 1435, 1436
NmeAIII	GCCGAG	2 cut(s)	645, 868
NmuCI	GTSAC	2 cut(s)	832, 1150
NspI	RCATGY	1 cut(s)	1066
NspV	TTCGAA	1 cut(s)	918
PauI	GCGCGC	1 cut(s)	640
PcsI	WCGNNNNNNNCGW	9 cut(s)	26, 35, 231, 522, 555, 672, 1029, 1074, 1474
PctI	GAATGC	1 cut(s)	486
PdiI	GCCGGC	1 cut(s)	316
PdmI	GAANNNNTTC	1 cut(s)	737
PfeI	GAWTC	5 cut(s)	123, 792, 887, 1054, 1071
PflFI	GACNNNGTC	2 cut(s)	34, 775
PfoI	TCCNGGA	1 cut(s)	950
PleI	GAGTC	6 cut(s)	151, 173, 181, 676, 784, 920
PluTI	GGCGCC	3 cut(s)	321, 445, 1202
PmaCI	CACGTG	1 cut(s)	458
PmlI	CACGTG	1 cut(s)	458
PpsI	GAGTC	6 cut(s)	151, 173, 181, 676, 784, 920
Ppu21I	YACGTR	2 cut(s)	458, 564
PpuMI	RGGWCCY	1 cut(s)	1003
PshAI	GACNNNNGTC	1 cut(s)	809
Psp124BI	GAGCTC	3 cut(s)	386, 524, 849
Psp5II	RGGWCCY	1 cut(s)	1003
Psp6I	CCWGG	3 cut(s)	189, 428, 1369
PspCI	CACGTG	1 cut(s)	458
PspGI	CCWGG	3 cut(s)	189, 428, 1369
PspN4I	GGNNCC	7 cut(s)	319, 353, 443, 950, 1200, 1435, 1436
PspOMI	GGGCCC	1 cut(s)	1433
PspPPI	RGGWCCY	1 cut(s)	1003
PstI	CTGCAG	1 cut(s)	336
PsuI	RGATCY	5 cut(s)	162, 210, 501, 570, 633
PsyI	GACNNNGTC	2 cut(s)	34, 775
PteI	GCGCGC	1 cut(s)	640
RsaI	GTAC	2 cut(s)	566, 1226
RsaNI	GTAC	2 cut(s)	565, 1225
RseI	CAYNNNNRTG	1 cut(s)	467
SacI	GAGCTC	3 cut(s)	386, 524, 849
SacII	CCGCGG	1 cut(s)	1169
SalI	GTCGAC	1 cut(s)	1145
SaqAI	TTAA	4 cut(s)	278, 1274, 1442, 1523
Sau3AI	GATC	9 cut(s)	151, 162, 210, 390, 399, 501, 570, 633, 646
SchI	GAGTC	6 cut(s)	151, 173, 182, 676, 785, 921
SduI	GDGCHC	6 cut(s)	225, 386, 524, 717, 849, 1437
SfcI	CTRYAG	1 cut(s)	332
SfoI	GGCGCC	3 cut(s)	319, 443, 1200
Sfr303I	CCGCGG	1 cut(s)	1169
SfuI	TTCGAA	1 cut(s)	918
SgrAI	CRCCGGYG	1 cut(s)	314
SgrBI	CCGCGG	1 cut(s)	1169
SgrDI	CGTCGACG	1 cut(s)	1145
SinI	GGWCC	7 cut(s)	858, 948, 1003, 1111, 1173, 1204, 1261
SmiMI	CAYNNNNRTG	1 cut(s)	467
SmlI	CTYRAG	4 cut(s)	50, 407, 1441, 1522
SmoI	CTYRAG	4 cut(s)	50, 407, 1441, 1522
Sse9I	AATT	1 cut(s)	862
SspDI	GGCGCC	3 cut(s)	317, 441, 1198
SspMI	CTAG	2 cut(s)	1013, 1019
SstI	GAGCTC	3 cut(s)	386, 524, 849
StyI	CCWWGG	1 cut(s)	1176
TaaI	ACNGT	3 cut(s)	1037, 1094, 1189
TaqI	TCGA	7 cut(s)	29, 225, 516, 621, 741, 918, 1146
TaqII	GACCGA	2 cut(s)	788, 899
TasI	AATT	1 cut(s)	862
TfiI	GAWTC	5 cut(s)	123, 792, 887, 1054, 1071
Tru1I	TTAA	4 cut(s)	278, 1274, 1442, 1523
Tru9I	TTAA	4 cut(s)	278, 1274, 1442, 1523
TscAI	CASTG	1 cut(s)	711
TseFI	GTSAC	2 cut(s)	832, 1150
TseI	GCWGC	5 cut(s)	331, 472, 1288, 1291, 1348
Tsp45I	GTSAC	2 cut(s)	832, 1150
TspDTI	ATGAA	2 cut(s)	1090, 1400
TspGWI	ACGGA	9 cut(s)	514, 522, 543, 600, 880, 1237, 1260, 1274, 1295
TspRI	CASTG	1 cut(s)	711
Tth111I	GACNNNGTC	2 cut(s)	34, 775
Vha464I	CTTAAG	2 cut(s)	1441, 1522
VpaK11BI	GGWCC	7 cut(s)	858, 948, 1003, 1111, 1173, 1204, 1261
XbaI	TCTAGA	1 cut(s)	1012
XceI	RCATGY	1 cut(s)	1066
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1445
XmiI	GTMKAC	2 cut(s)	813, 1146
XmnI	GAANNNNTTC	1 cut(s)	737
XspI	CTAG	2 cut(s)	1013, 1019
ZraI	GACGTC	2 cut(s)	238, 832

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.